AlfalfaGEDB | Msa0739010

M. sativa cultivar zhongmu-4 / Msa0739010

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0020630	Msa0739010	0.804355	2.247843e-49	-8.615850e-47
Msa0027540	Msa0739010	0.809932	1.481582e-50	-8.615850e-47
Msa0030380	Msa0739010	0.843327	1.499998e-58	-8.615850e-47
Msa0044710	Msa0739010	0.813380	2.635455e-51	-8.615850e-47
Msa0075310	Msa0739010	0.809041	2.301979e-50	-8.615850e-47
Msa0084680	Msa0739010	0.804674	1.928532e-49	-8.615850e-47
Msa0618010	Msa0739010	0.811132	8.157371e-51	-8.615850e-47
Msa0621120	Msa0739010	0.808245	3.405283e-50	-8.615850e-47
Msa0669220	Msa0739010	0.819088	1.395095e-52	-8.615850e-47
Msa0677270	Msa0739010	0.802437	5.611585e-49	-8.615850e-47
Msa0734660	Msa0739010	0.816731	4.754123e-52	-8.615850e-47
Msa0739010	Msa0776220	0.836659	8.220377e-57	-8.615850e-47
Msa0739010	Msa0813720	0.810959	8.891786e-51	-8.615850e-47
Msa0739010	Msa0854120	0.819493	1.128307e-52	-8.615850e-47
Msa0739010	Msa0854570	0.800439	1.440193e-48	-8.615850e-47
Msa0739010	Msa0950320	0.807190	5.704617e-50	-8.615850e-47
Msa0739010	Msa1111630	0.800115	1.676774e-48	-8.615850e-47
Msa0739010	Msa1159990	0.806199	9.236850e-50	-8.615850e-47
Msa0739010	Msa1177240	0.800083	1.701916e-48	-8.615850e-47
Msa0739010	Msa1210770	0.822589	2.185374e-53	-8.615850e-47
Msa0739010	Msa1317030	0.802651	5.070481e-49	-8.615850e-47
Msa0739010	Msa1427830	0.800588	1.343003e-48	-8.615850e-47
Msa0739010	Msa1449880	0.818043	2.407862e-52	-8.615850e-47
Msa0739010	Msa1453620	0.818826	1.600770e-52	-8.615850e-47
Msa0739010	Msa1454110	0.815303	9.905540e-52	-8.615850e-47
Msa0159750	Msa0739010	0.803866	2.841193e-49	-8.615850e-47
Msa0169620	Msa0739010	0.815503	8.941370e-52	-8.615850e-47
Msa0173740	Msa0739010	0.838102	3.510718e-57	-8.615850e-47
Msa0173820	Msa0739010	0.836765	7.724064e-57	-8.615850e-47
Msa0173830	Msa0739010	0.844090	9.375077e-59	-8.615850e-47
Msa0182930	Msa0739010	0.825104	5.622674e-54	-8.615850e-47
Msa0207000	Msa0739010	0.809509	1.827274e-50	-8.615850e-47
Msa0378080	Msa0739010	0.802991	4.313657e-49	-8.615850e-47
Msa0244270	Msa0739010	0.806764	7.020082e-50	-8.615850e-47
Msa0280490	Msa0739010	0.805313	1.417871e-49	-8.615850e-47
Msa0288240	Msa0739010	0.808092	3.670874e-50	-8.615850e-47
Msa0298970	Msa0739010	0.804432	2.166478e-49	-8.615850e-47
Msa0301180	Msa0739010	0.810866	9.314026e-51	-8.615850e-47
Msa0524120	Msa0739010	0.815793	7.704616e-52	-8.615850e-47
Msa0564930	Msa0739010	0.804233	2.383337e-49	-8.615850e-47
Msa0571700	Msa0739010	0.803900	2.795838e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 14 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAATGATTTAGACCTAAAT+AGG	0.216099	5_1:+57046158	Msa0739010:three_prime_UTR
TATCTAAATTTAGATGAATT+TGG	0.257410	5_1:+57046129	Msa0739010:exon
TTAGGTCTAAATCATTGTTT+TGG	0.287050	5_1:-57046153	None:intergenic
CAGAGCGTGTATGCAGTTCT+AGG	0.298180	5_1:-57045361	None:intergenic
GCTCATTGATACACGGATAA+TGG	0.383000	5_1:-57045989	None:intergenic
TTAAACATGTTTACAACAAA+AGG	0.398188	5_1:-57045398	None:intergenic
TTCATCAAGATACAATATAG+AGG	0.465852	5_1:+57045482	Msa0739010:five_prime_UTR
GATTTGAAGCCTTCTGTAAG+TGG	0.478640	5_1:-57045928	None:intergenic
GTCCAAGACTTCAGCAAAAG+TGG	0.482390	5_1:+57045903	Msa0739010:five_prime_UTR
ACTGGTTACATTCTTTCTCT+AGG	0.487663	5_1:-57046013	None:intergenic
CTTCAAATCACAGTACACAT+AGG	0.575742	5_1:+57045942	Msa0739010:five_prime_UTR
AAAGTGGTTCCACTTACAGA+AGG	0.615621	5_1:+57045919	Msa0739010:five_prime_UTR
TCTAGGTGCTCATTGATACA+CGG	0.643560	5_1:-57045996	None:intergenic
AGCTCATTGAAAATGCTCAG+TGG	0.703675	5_1:+57045427	Msa0739010:five_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATTTTATTCAATGATTTG+TGG	-	chr5_1:57045839-57045858	None:intergenic	15.0%
!!	TATCTAAATTTAGATGAATT+TGG	+	chr5_1:57046129-57046148	Msa0739010:exon	15.0%
!!!	TTATTTTATTCAATGATTTG+TGG	-	chr5_1:57045839-57045858	None:intergenic	15.0%
!!	TATCTAAATTTAGATGAATT+TGG	+	chr5_1:57046129-57046148	Msa0739010:exon	15.0%
!!!	TAGGTTTTTTTATCTTGAAA+TGG	-	chr5_1:57045345-57045364	None:intergenic	20.0%
!!	TTAAACATGTTTACAACAAA+AGG	-	chr5_1:57045401-57045420	None:intergenic	20.0%
!!!	TAACATTCTTTTTCATAAAC+TGG	-	chr5_1:57046034-57046053	None:intergenic	20.0%
!!!	TAGGTTTTTTTATCTTGAAA+TGG	-	chr5_1:57045345-57045364	None:intergenic	20.0%
!!	TTAAACATGTTTACAACAAA+AGG	-	chr5_1:57045401-57045420	None:intergenic	20.0%
!!!	TAACATTCTTTTTCATAAAC+TGG	-	chr5_1:57046034-57046053	None:intergenic	20.0%
!	TTCATCAAGATACAATATAG+AGG	+	chr5_1:57045482-57045501	Msa0739010:five_prime_UTR	25.0%
!	CTATTTAGATGTAAAACTCT+TGG	+	chr5_1:57045577-57045596	Msa0739010:intron	25.0%
!	TGCAAAATGCAAAATTAATG+CGG	-	chr5_1:57045797-57045816	None:intergenic	25.0%
!!!	AAAATAAGCATTTTAACTGC+AGG	+	chr5_1:57045852-57045871	Msa0739010:intron	25.0%
!!	TTAGGTCTAAATCATTGTTT+TGG	-	chr5_1:57046156-57046175	None:intergenic	25.0%
!	ACAATGATTTAGACCTAAAT+AGG	+	chr5_1:57046158-57046177	Msa0739010:three_prime_UTR	25.0%
!	TTCATCAAGATACAATATAG+AGG	+	chr5_1:57045482-57045501	Msa0739010:five_prime_UTR	25.0%
!	CTATTTAGATGTAAAACTCT+TGG	+	chr5_1:57045577-57045596	Msa0739010:intron	25.0%
!	TGCAAAATGCAAAATTAATG+CGG	-	chr5_1:57045797-57045816	None:intergenic	25.0%
!!!	AAAATAAGCATTTTAACTGC+AGG	+	chr5_1:57045852-57045871	Msa0739010:intron	25.0%
!!	TTAGGTCTAAATCATTGTTT+TGG	-	chr5_1:57046156-57046175	None:intergenic	25.0%
	TTTAGATGTAAAACTCTTGG+CGG	+	chr5_1:57045580-57045599	Msa0739010:intron	30.0%
	TTGTTCAAAATAGTGCATTG+CGG	-	chr5_1:57045690-57045709	None:intergenic	30.0%
	GTTCAAATTCTGCTATACTA+TGG	-	chr5_1:57045728-57045747	None:intergenic	30.0%
	TTTAGATGTAAAACTCTTGG+CGG	+	chr5_1:57045580-57045599	Msa0739010:intron	30.0%
	TTGTTCAAAATAGTGCATTG+CGG	-	chr5_1:57045690-57045709	None:intergenic	30.0%
	GTTCAAATTCTGCTATACTA+TGG	-	chr5_1:57045728-57045747	None:intergenic	30.0%
	TTAGATGTAAAACTCTTGGC+GGG	+	chr5_1:57045581-57045600	Msa0739010:intron	35.0%
	CTTCAAATCACAGTACACAT+AGG	+	chr5_1:57045942-57045961	Msa0739010:five_prime_UTR	35.0%
	ACTGGTTACATTCTTTCTCT+AGG	-	chr5_1:57046016-57046035	None:intergenic	35.0%
	TTAGATGTAAAACTCTTGGC+GGG	+	chr5_1:57045581-57045600	Msa0739010:intron	35.0%
	CTTCAAATCACAGTACACAT+AGG	+	chr5_1:57045942-57045961	Msa0739010:five_prime_UTR	35.0%
	ACTGGTTACATTCTTTCTCT+AGG	-	chr5_1:57046016-57046035	None:intergenic	35.0%
	AGCTCATTGAAAATGCTCAG+TGG	+	chr5_1:57045427-57045446	Msa0739010:five_prime_UTR	40.0%
	ATAGACTTGCATCCCTCAAA+TGG	+	chr5_1:57045537-57045556	Msa0739010:intron	40.0%
	TAGACTTGCATCCCTCAAAT+GGG	+	chr5_1:57045538-57045557	Msa0739010:intron	40.0%
	TGCACATATGACCCATTTGA+GGG	-	chr5_1:57045552-57045571	None:intergenic	40.0%
	ATGCACATATGACCCATTTG+AGG	-	chr5_1:57045553-57045572	None:intergenic	40.0%
	TAGATGTAAAACTCTTGGCG+GGG	+	chr5_1:57045582-57045601	Msa0739010:intron	40.0%
	TAGCAGCTGTATGACAACAT+TGG	-	chr5_1:57045614-57045633	None:intergenic	40.0%
	TGTTGTCATACAGCTGCTAT+AGG	+	chr5_1:57045615-57045634	Msa0739010:intron	40.0%
	GTTGTCATACAGCTGCTATA+GGG	+	chr5_1:57045616-57045635	Msa0739010:intron	40.0%
!	GCTGTATTGTTATTGCGCAA+TGG	+	chr5_1:57045641-57045660	Msa0739010:intron	40.0%
	CTAGAGGTTAATGTCAATCG+TGG	-	chr5_1:57045769-57045788	None:intergenic	40.0%
!!	AATTAATGCGGTGCTACTAG+AGG	-	chr5_1:57045785-57045804	None:intergenic	40.0%
!!	AACCACTTTTGCTGAAGTCT+TGG	-	chr5_1:57045908-57045927	None:intergenic	40.0%
	AAAGTGGTTCCACTTACAGA+AGG	+	chr5_1:57045919-57045938	Msa0739010:five_prime_UTR	40.0%
!	GATTTGAAGCCTTCTGTAAG+TGG	-	chr5_1:57045931-57045950	None:intergenic	40.0%
	GCTCATTGATACACGGATAA+TGG	-	chr5_1:57045992-57046011	None:intergenic	40.0%
	TCTAGGTGCTCATTGATACA+CGG	-	chr5_1:57045999-57046018	None:intergenic	40.0%
	AGCTCATTGAAAATGCTCAG+TGG	+	chr5_1:57045427-57045446	Msa0739010:five_prime_UTR	40.0%
	ATAGACTTGCATCCCTCAAA+TGG	+	chr5_1:57045537-57045556	Msa0739010:intron	40.0%
	TAGACTTGCATCCCTCAAAT+GGG	+	chr5_1:57045538-57045557	Msa0739010:intron	40.0%
	TGCACATATGACCCATTTGA+GGG	-	chr5_1:57045552-57045571	None:intergenic	40.0%
	ATGCACATATGACCCATTTG+AGG	-	chr5_1:57045553-57045572	None:intergenic	40.0%
	TAGATGTAAAACTCTTGGCG+GGG	+	chr5_1:57045582-57045601	Msa0739010:intron	40.0%
	TAGCAGCTGTATGACAACAT+TGG	-	chr5_1:57045614-57045633	None:intergenic	40.0%
	TGTTGTCATACAGCTGCTAT+AGG	+	chr5_1:57045615-57045634	Msa0739010:intron	40.0%
	GTTGTCATACAGCTGCTATA+GGG	+	chr5_1:57045616-57045635	Msa0739010:intron	40.0%
!	GCTGTATTGTTATTGCGCAA+TGG	+	chr5_1:57045641-57045660	Msa0739010:intron	40.0%
	CTAGAGGTTAATGTCAATCG+TGG	-	chr5_1:57045769-57045788	None:intergenic	40.0%
!!	AATTAATGCGGTGCTACTAG+AGG	-	chr5_1:57045785-57045804	None:intergenic	40.0%
!!	AACCACTTTTGCTGAAGTCT+TGG	-	chr5_1:57045908-57045927	None:intergenic	40.0%
	AAAGTGGTTCCACTTACAGA+AGG	+	chr5_1:57045919-57045938	Msa0739010:five_prime_UTR	40.0%
!	GATTTGAAGCCTTCTGTAAG+TGG	-	chr5_1:57045931-57045950	None:intergenic	40.0%
	GCTCATTGATACACGGATAA+TGG	-	chr5_1:57045992-57046011	None:intergenic	40.0%
	TCTAGGTGCTCATTGATACA+CGG	-	chr5_1:57045999-57046018	None:intergenic	40.0%
	AGATGTAAAACTCTTGGCGG+GGG	+	chr5_1:57045583-57045602	Msa0739010:intron	45.0%
	GTCCAAGACTTCAGCAAAAG+TGG	+	chr5_1:57045903-57045922	Msa0739010:five_prime_UTR	45.0%
	AGATGTAAAACTCTTGGCGG+GGG	+	chr5_1:57045583-57045602	Msa0739010:intron	45.0%
	GTCCAAGACTTCAGCAAAAG+TGG	+	chr5_1:57045903-57045922	Msa0739010:five_prime_UTR	45.0%
	CAGAGCGTGTATGCAGTTCT+AGG	-	chr5_1:57045364-57045383	None:intergenic	50.0%
	CAGAGCGTGTATGCAGTTCT+AGG	-	chr5_1:57045364-57045383	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	57045316	57046180	57045316	ID=Msa0739010;Name=Msa0739010
chr5_1	mRNA	57045316	57046180	57045316	ID=Msa0739010-mRNA-1;Parent=Msa0739010;Name=Msa0739010-mRNA-1;_AED=0.00;_eAED=0.00;_QI=352\|1\|1\|1\|1\|1\|2\|35\|35
chr5_1	exon	57045316	57045503	57045316	ID=Msa0739010-mRNA-1:exon:16114;Parent=Msa0739010-mRNA-1
chr5_1	exon	57045874	57046180	57045874	ID=Msa0739010-mRNA-1:exon:16115;Parent=Msa0739010-mRNA-1
chr5_1	five_prime_UTR	57045316	57045503	57045316	ID=Msa0739010-mRNA-1:five_prime_utr;Parent=Msa0739010-mRNA-1
chr5_1	five_prime_UTR	57045874	57046037	57045874	ID=Msa0739010-mRNA-1:five_prime_utr;Parent=Msa0739010-mRNA-1
chr5_1	CDS	57046038	57046145	57046038	ID=Msa0739010-mRNA-1:cds;Parent=Msa0739010-mRNA-1
chr5_1	three_prime_UTR	57046146	57046180	57046146	ID=Msa0739010-mRNA-1:three_prime_utr;Parent=Msa0739010-mRNA-1

Gene Sequence	>Msa0739010 ATGAAAAAGAATGTTACTAATTTCATTGTTTCTTGTAAACTAGTGAATTGTAACTGTAATGTAATTACAATTAGTTCAGCGCTAGAGATGTTATCTAAATTTAGATGA
Protein sequence	>Msa0739010 MKKNVTNFIVSCKLVNCNCNVITISSALEMLSKFR*