AlfalfaGEDB | Msa0757320

M. sativa cultivar zhongmu-4 / Msa0757320

KEGG: K01265
GO: GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0019538 GO:0031365 GO:0033993 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071704 GO:0097305 GO:1901564 GO:1901700
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0757320	GAU17376.1	98.246	57	1	0	1	57	296	352	1.64e-33	127

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0757320	sp\|Q9FV50\|MAP1D_ARATH	82.456	57	10	0	1	57	294	350	5.27e-30	110

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0757320	A0A2Z6MMR8	98.246	57	1	0	1	57	296	352	7.82e-34	127

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0075310	Msa0757320	0.808436	3.100530e-50	-8.615850e-47
Msa0091770	Msa0757320	0.807144	5.834675e-50	-8.615850e-47
Msa0094100	Msa0757320	0.817370	3.415679e-52	-8.615850e-47
Msa0621890	Msa0757320	0.807746	4.348639e-50	-8.615850e-47
Msa0629370	Msa0757320	0.813654	2.294434e-51	-8.615850e-47
Msa0638280	Msa0757320	0.802650	5.072663e-49	-8.615850e-47
Msa0638320	Msa0757320	0.816321	5.873857e-52	-8.615850e-47
Msa0677270	Msa0757320	0.809356	1.970229e-50	-8.615850e-47
Msa0697140	Msa0757320	0.810293	1.238815e-50	-8.615850e-47
Msa0730180	Msa0757320	0.807620	4.625358e-50	-8.615850e-47
Msa0757320	Msa0789270	0.814185	1.752299e-51	-8.615850e-47
Msa0757320	Msa0789280	0.820467	6.754696e-53	-8.615850e-47
Msa0757320	Msa0789290	0.820321	7.297811e-53	-8.615850e-47
Msa0757320	Msa0789300	0.818073	2.370398e-52	-8.615850e-47
Msa0757320	Msa0845590	-0.800381	1.479968e-48	-8.615850e-47
Msa0757320	Msa0854570	0.806963	6.372783e-50	-8.615850e-47
Msa0757320	Msa0870620	0.807231	5.590768e-50	-8.615850e-47
Msa0757320	Msa0870630	0.823842	1.114107e-53	-8.615850e-47
Msa0757320	Msa0903960	0.832468	9.292263e-56	-8.615850e-47
Msa0757320	Msa0936270	0.823038	1.717531e-53	-8.615850e-47
Msa0757320	Msa1008490	0.802010	6.871844e-49	-8.615850e-47
Msa0757320	Msa1160400	0.804183	2.440976e-49	-8.615850e-47
Msa0757320	Msa1172590	0.810103	1.361620e-50	-8.615850e-47
Msa0757320	Msa1226510	0.802994	4.308066e-49	-8.615850e-47
Msa0757320	Msa1262310	0.804560	2.037197e-49	-8.615850e-47
Msa0757320	Msa1284140	0.817152	3.825139e-52	-8.615850e-47
Msa0757320	Msa1297090	0.808365	3.210805e-50	-8.615850e-47
Msa0757320	Msa1321000	0.818190	2.230763e-52	-8.615850e-47
Msa0757320	Msa1408790	0.803931	2.753974e-49	-8.615850e-47
Msa0757320	Msa1431000	0.802817	4.685526e-49	-8.615850e-47
Msa0757320	Msa1436040	0.804333	2.271630e-49	-8.615850e-47
Msa0757320	Msa1464560	0.813312	2.728313e-51	-8.615850e-47
Msa0168100	Msa0757320	0.803430	3.499598e-49	-8.615850e-47
Msa0170580	Msa0757320	0.803153	3.993736e-49	-8.615850e-47
Msa0208520	Msa0757320	0.807342	5.296859e-50	-8.615850e-47
Msa0208630	Msa0757320	0.814695	1.351602e-51	-8.615850e-47
Msa0398500	Msa0757320	0.810586	1.070968e-50	-8.615850e-47
Msa0440110	Msa0757320	0.803130	4.037388e-49	-8.615850e-47
Msa0480320	Msa0757320	0.804993	1.654291e-49	-8.615850e-47
Msa0252180	Msa0757320	0.800781	1.227082e-48	-8.615850e-47
Msa0265820	Msa0757320	0.813395	2.615547e-51	-8.615850e-47
Msa0270270	Msa0757320	0.803795	2.939500e-49	-8.615850e-47
Msa0296760	Msa0757320	0.804149	2.481685e-49	-8.615850e-47
Msa0490350	Msa0757320	0.801883	7.296717e-49	-8.615850e-47
Msa0532300	Msa0757320	0.813174	2.924883e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0757320	MtrunA17_Chr5g0402401	98.246	57	1	1	57	295	351	3.04e-37	126
Msa0757320	MtrunA17_Chr5g0422531	64.912	57	20	1	57	300	356	5.98e-22	85.9
Msa0757320	MtrunA17_Chr4g0011731	56.364	55	24	1	55	328	382	2.01e-16	70.9

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0757320	AT4G37040.1	82.456	57	10	1	57	294	350	5.36e-31	110
Msa0757320	AT1G13270.1	59.649	57	23	1	57	313	369	1.70e-20	82.4
Msa0757320	AT3G25740.1	60.000	55	22	1	55	287	341	7.62e-18	74.7
Msa0757320	AT2G45240.1	56.364	55	24	1	55	329	383	6.98e-16	69.7

Find 23 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACAGAACCTATGCTGACTA+TGG	0.239457	5_2:+12146058	Msa0757320:intron
GGCACATGCTTCTTATAACT+TGG	0.349020	5_2:-12146320	None:intergenic
CAAGAACTTTGATCTGAAAC+TGG	0.356213	5_2:-12146286	None:intergenic
TTTCAGATCAAAGTTCTTGC+TGG	0.375544	5_2:+12146290	Msa0757320:three_prime_UTR
TCAATAGGTAGAGGGAGATT+AGG	0.389670	5_2:+12146446	Msa0757320:three_prime_UTR
TGCGAGCTATGCAAAGATAT+TGG	0.394431	5_2:-12146222	None:intergenic
CCCCATCAGGTGTTATCAAA+AGG	0.394761	5_2:-12146163	None:intergenic
CAATTGTCTTTCCACATCAC+TGG	0.411722	5_2:-12146092	None:intergenic
ATACAACACTGTGACTCATT+TGG	0.415013	5_2:-12146341	None:intergenic
TTCATTATAGGTTTGTCAAT+AGG	0.420180	5_2:+12146431	Msa0757320:three_prime_UTR
TATTCTTTCGATCATTAAGA+TGG	0.439056	5_2:+12146477	Msa0757320:three_prime_UTR
GTCATAATCTCAGCCCCATC+AGG	0.462099	5_2:-12146176	None:intergenic
CAGTGATGTGGAAAGACAAT+TGG	0.469081	5_2:+12146093	Msa0757320:CDS
TGGACAGTTGTAACAGAAGA+TGG	0.474162	5_2:+12146113	Msa0757320:CDS
TTAAATCAAACCTTCACGAT+TGG	0.513964	5_2:+12144311	Msa0757320:CDS
CCCATCAGGTGTTATCAAAA+GGG	0.517184	5_2:-12146162	None:intergenic
GCACATGCTTCTTATAACTT+GGG	0.520577	5_2:-12146319	None:intergenic
ATAGGTTTGTCAATAGGTAG+AGG	0.533551	5_2:+12146437	Msa0757320:three_prime_UTR
TAGGTTTGTCAATAGGTAGA+GGG	0.545585	5_2:+12146438	Msa0757320:three_prime_UTR
ACAGAACCTATGCTGACTAT+GGG	0.591367	5_2:+12146059	Msa0757320:intron
TGTGTTCAAACTGAGCTGAA+AGG	0.604770	5_2:-12146139	None:intergenic
ATGCTGCCCATAGTCAGCAT+AGG	0.627596	5_2:-12146065	None:intergenic
GCAGCATCAATCCAGTGATG+TGG	0.633825	5_2:+12146081	Msa0757320:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTCTTGTTATTTTTATTTTC+AGG	+	chr5_2:12144263-12144282	Msa0757320:five_prime_UTR	15.0%
!!	ATAACAAAATCTATAAATCT+TGG	-	chr5_2:12145996-12146015	None:intergenic	15.0%
!!!	ATTTAATTTTTTTCTTTACC+TGG	+	chr5_2:12146028-12146047	Msa0757320:intron	15.0%
!!!	TTCTTGTTATTTTTATTTTC+AGG	+	chr5_2:12144263-12144282	Msa0757320:five_prime_UTR	15.0%
!!	ATAACAAAATCTATAAATCT+TGG	-	chr5_2:12145996-12146015	None:intergenic	15.0%
!!!	ATTTAATTTTTTTCTTTACC+TGG	+	chr5_2:12146028-12146047	Msa0757320:intron	15.0%
!!	AAATATTGAATTGTGACATT+AGG	-	chr5_2:12144559-12144578	None:intergenic	20.0%
!!!	ACACTAAAACTTGTTAAAAA+GGG	-	chr5_2:12144971-12144990	None:intergenic	20.0%
!!!	TCAAAATTTGTAAAGTACTT+TGG	+	chr5_2:12145428-12145447	Msa0757320:intron	20.0%
!!!	GTTTTTCATATATAGAGTTA+AGG	+	chr5_2:12145871-12145890	Msa0757320:intron	20.0%
!!	CTTAAGTCTATATATAATCA+TGG	+	chr5_2:12145907-12145926	Msa0757320:intron	20.0%
!!!	CTACACTTATTTTTCATTAT+AGG	+	chr5_2:12146419-12146438	Msa0757320:three_prime_UTR	20.0%
!!	AAGAATAATTGTTACAAACA+GGG	-	chr5_2:12144248-12144267	None:intergenic	20.0%
!!	AAATATTGAATTGTGACATT+AGG	-	chr5_2:12144559-12144578	None:intergenic	20.0%
!!!	ACACTAAAACTTGTTAAAAA+GGG	-	chr5_2:12144971-12144990	None:intergenic	20.0%
!!!	TCAAAATTTGTAAAGTACTT+TGG	+	chr5_2:12145428-12145447	Msa0757320:intron	20.0%
!!!	GTTTTTCATATATAGAGTTA+AGG	+	chr5_2:12145871-12145890	Msa0757320:intron	20.0%
!!	CTTAAGTCTATATATAATCA+TGG	+	chr5_2:12145907-12145926	Msa0757320:intron	20.0%
!!!	CTACACTTATTTTTCATTAT+AGG	+	chr5_2:12146419-12146438	Msa0757320:three_prime_UTR	20.0%
!	AAAAATATTACCAATCGTGA+AGG	-	chr5_2:12144324-12144343	None:intergenic	25.0%
!	CATAACAGTCTATATTGTAT+TGG	-	chr5_2:12144351-12144370	None:intergenic	25.0%
!!!	GACACTAAAACTTGTTAAAA+AGG	-	chr5_2:12144972-12144991	None:intergenic	25.0%
!!	GGAAATAATGATTTTACTCT+TGG	+	chr5_2:12145117-12145136	Msa0757320:intron	25.0%
!	AAAGAGCTCAATTTCAATTT+GGG	-	chr5_2:12145257-12145276	None:intergenic	25.0%
!	TAAAGAGCTCAATTTCAATT+TGG	-	chr5_2:12145258-12145277	None:intergenic	25.0%
!	AATAAGAAAGTAGAGATTGA+GGG	-	chr5_2:12145362-12145381	None:intergenic	25.0%
!	AAATAAGAAAGTAGAGATTG+AGG	-	chr5_2:12145363-12145382	None:intergenic	25.0%
!!	TTGTAAAGTACTTTGGTATT+TGG	+	chr5_2:12145435-12145454	Msa0757320:intron	25.0%
!!	TGTAAAGTACTTTGGTATTT+GGG	+	chr5_2:12145436-12145455	Msa0757320:intron	25.0%
!!!	TAAACTTTTTGAAAACCCAT+CGG	+	chr5_2:12145483-12145502	Msa0757320:intron	25.0%
!!!	TTTTCTTTCCCTTATTTGTT+AGG	+	chr5_2:12145547-12145566	Msa0757320:intron	25.0%
!	AACATACACCTAACAAATAA+GGG	-	chr5_2:12145558-12145577	None:intergenic	25.0%
!	AAACATACACCTAACAAATA+AGG	-	chr5_2:12145559-12145578	None:intergenic	25.0%
!	ATTAAGAGTTATTGCATTAG+AGG	+	chr5_2:12145643-12145662	Msa0757320:intron	25.0%
!	AGAAGAAGACAAAAAACTAT+AGG	+	chr5_2:12145667-12145686	Msa0757320:intron	25.0%
!	TATAGACTTAAGAGTCTATT+AGG	-	chr5_2:12145899-12145918	None:intergenic	25.0%
!	ACAAAATCTATAAATCTTGG+AGG	-	chr5_2:12145993-12146012	None:intergenic	25.0%
!	TTCATTATAGGTTTGTCAAT+AGG	+	chr5_2:12146431-12146450	Msa0757320:three_prime_UTR	25.0%
!	TATTCTTTCGATCATTAAGA+TGG	+	chr5_2:12146477-12146496	Msa0757320:three_prime_UTR	25.0%
!	CAAGAATAATTGTTACAAAC+AGG	-	chr5_2:12144249-12144268	None:intergenic	25.0%
!	AAAAATATTACCAATCGTGA+AGG	-	chr5_2:12144324-12144343	None:intergenic	25.0%
!	CATAACAGTCTATATTGTAT+TGG	-	chr5_2:12144351-12144370	None:intergenic	25.0%
!!!	GACACTAAAACTTGTTAAAA+AGG	-	chr5_2:12144972-12144991	None:intergenic	25.0%
!!	GGAAATAATGATTTTACTCT+TGG	+	chr5_2:12145117-12145136	Msa0757320:intron	25.0%
!	AAAGAGCTCAATTTCAATTT+GGG	-	chr5_2:12145257-12145276	None:intergenic	25.0%
!	TAAAGAGCTCAATTTCAATT+TGG	-	chr5_2:12145258-12145277	None:intergenic	25.0%
!	AATAAGAAAGTAGAGATTGA+GGG	-	chr5_2:12145362-12145381	None:intergenic	25.0%
!	AAATAAGAAAGTAGAGATTG+AGG	-	chr5_2:12145363-12145382	None:intergenic	25.0%
!!	TTGTAAAGTACTTTGGTATT+TGG	+	chr5_2:12145435-12145454	Msa0757320:intron	25.0%
!!	TGTAAAGTACTTTGGTATTT+GGG	+	chr5_2:12145436-12145455	Msa0757320:intron	25.0%
!!!	TAAACTTTTTGAAAACCCAT+CGG	+	chr5_2:12145483-12145502	Msa0757320:intron	25.0%
!!!	TTTTCTTTCCCTTATTTGTT+AGG	+	chr5_2:12145547-12145566	Msa0757320:intron	25.0%
!	AACATACACCTAACAAATAA+GGG	-	chr5_2:12145558-12145577	None:intergenic	25.0%
!	AAACATACACCTAACAAATA+AGG	-	chr5_2:12145559-12145578	None:intergenic	25.0%
!	ATTAAGAGTTATTGCATTAG+AGG	+	chr5_2:12145643-12145662	Msa0757320:intron	25.0%
!	AGAAGAAGACAAAAAACTAT+AGG	+	chr5_2:12145667-12145686	Msa0757320:intron	25.0%
!	TATAGACTTAAGAGTCTATT+AGG	-	chr5_2:12145899-12145918	None:intergenic	25.0%
!	ACAAAATCTATAAATCTTGG+AGG	-	chr5_2:12145993-12146012	None:intergenic	25.0%
!	TTCATTATAGGTTTGTCAAT+AGG	+	chr5_2:12146431-12146450	Msa0757320:three_prime_UTR	25.0%
!	TATTCTTTCGATCATTAAGA+TGG	+	chr5_2:12146477-12146496	Msa0757320:three_prime_UTR	25.0%
	TTAAATCAAACCTTCACGAT+TGG	+	chr5_2:12144311-12144330	Msa0757320:CDS	30.0%
	ATGATTATTGTTTGCAAAGC+TGG	+	chr5_2:12144382-12144401	Msa0757320:intron	30.0%
	AAAGGAAAGAGAATTTCTCA+AGG	-	chr5_2:12144624-12144643	None:intergenic	30.0%
	CAAATTTAAATTTGGCAGTG+TGG	-	chr5_2:12144743-12144762	None:intergenic	30.0%
	CACACTGCCAAATTTAAATT+TGG	+	chr5_2:12144741-12144760	Msa0757320:intron	30.0%
	GCAGCTTCCAAATTTAAATT+TGG	-	chr5_2:12144751-12144770	None:intergenic	30.0%
	AAATAAGTGGCTTACATACA+TGG	-	chr5_2:12144818-12144837	None:intergenic	30.0%
!	TGTATGTAAGCCACTTATTT+TGG	+	chr5_2:12144818-12144837	Msa0757320:intron	30.0%
	CACAATTACTCCAAAATAAG+TGG	-	chr5_2:12144831-12144850	None:intergenic	30.0%
	AACTGCCTTTGAATTTACAT+AGG	+	chr5_2:12144854-12144873	Msa0757320:intron	30.0%
	CTTAGCCTATGTAAATTCAA+AGG	-	chr5_2:12144862-12144881	None:intergenic	30.0%
!	GTAAAAATAATGGAAGTGAG+TGG	+	chr5_2:12145096-12145115	Msa0757320:intron	30.0%
	TGAAAATGATCTGACAATGA+AGG	-	chr5_2:12145204-12145223	None:intergenic	30.0%
!	TCAGATCATTTTCACCTTAA+AGG	+	chr5_2:12145210-12145229	Msa0757320:intron	30.0%
!!	TTTTGAAAACATGACACCAA+GGG	-	chr5_2:12145414-12145433	None:intergenic	30.0%
!!	ATTTTGAAAACATGACACCA+AGG	-	chr5_2:12145415-12145434	None:intergenic	30.0%
!	GTAAAGTACTTTGGTATTTG+GGG	+	chr5_2:12145437-12145456	Msa0757320:intron	30.0%
	TAGTTCATGATGTCTATATG+AGG	+	chr5_2:12145506-12145525	Msa0757320:intron	30.0%
	AGTTCATGATGTCTATATGA+GGG	+	chr5_2:12145507-12145526	Msa0757320:intron	30.0%
!	ACAATGTTGCATTTTATGAG+CGG	+	chr5_2:12145601-12145620	Msa0757320:intron	30.0%
	ACTATAGGCTAAAGATTTAG+AGG	+	chr5_2:12145682-12145701	Msa0757320:intron	30.0%
	CGATCATAAATCATGTCTAT+AGG	-	chr5_2:12145719-12145738	None:intergenic	30.0%
!!	ATCACATTTTGAAGAAACTG+TGG	+	chr5_2:12145840-12145859	Msa0757320:intron	30.0%
	CTCACTAAAGCAAGTTTAAA+GGG	-	chr5_2:12145962-12145981	None:intergenic	30.0%
	TCTCACTAAAGCAAGTTTAA+AGG	-	chr5_2:12145963-12145982	None:intergenic	30.0%
	TAGGTTCTGTAAAATTTACC+AGG	-	chr5_2:12146049-12146068	None:intergenic	30.0%
	TTAAATCAAACCTTCACGAT+TGG	+	chr5_2:12144311-12144330	Msa0757320:CDS	30.0%
	ATGATTATTGTTTGCAAAGC+TGG	+	chr5_2:12144382-12144401	Msa0757320:intron	30.0%
	AAAGGAAAGAGAATTTCTCA+AGG	-	chr5_2:12144624-12144643	None:intergenic	30.0%
	CAAATTTAAATTTGGCAGTG+TGG	-	chr5_2:12144743-12144762	None:intergenic	30.0%
	CACACTGCCAAATTTAAATT+TGG	+	chr5_2:12144741-12144760	Msa0757320:intron	30.0%
	GCAGCTTCCAAATTTAAATT+TGG	-	chr5_2:12144751-12144770	None:intergenic	30.0%
	AAATAAGTGGCTTACATACA+TGG	-	chr5_2:12144818-12144837	None:intergenic	30.0%
!	TGTATGTAAGCCACTTATTT+TGG	+	chr5_2:12144818-12144837	Msa0757320:intron	30.0%
	CACAATTACTCCAAAATAAG+TGG	-	chr5_2:12144831-12144850	None:intergenic	30.0%
	AACTGCCTTTGAATTTACAT+AGG	+	chr5_2:12144854-12144873	Msa0757320:intron	30.0%
	CTTAGCCTATGTAAATTCAA+AGG	-	chr5_2:12144862-12144881	None:intergenic	30.0%
!	GTAAAAATAATGGAAGTGAG+TGG	+	chr5_2:12145096-12145115	Msa0757320:intron	30.0%
	TGAAAATGATCTGACAATGA+AGG	-	chr5_2:12145204-12145223	None:intergenic	30.0%
!	TCAGATCATTTTCACCTTAA+AGG	+	chr5_2:12145210-12145229	Msa0757320:intron	30.0%
!!	TTTTGAAAACATGACACCAA+GGG	-	chr5_2:12145414-12145433	None:intergenic	30.0%
!!	ATTTTGAAAACATGACACCA+AGG	-	chr5_2:12145415-12145434	None:intergenic	30.0%
!	GTAAAGTACTTTGGTATTTG+GGG	+	chr5_2:12145437-12145456	Msa0757320:intron	30.0%
	TAGTTCATGATGTCTATATG+AGG	+	chr5_2:12145506-12145525	Msa0757320:intron	30.0%
	AGTTCATGATGTCTATATGA+GGG	+	chr5_2:12145507-12145526	Msa0757320:intron	30.0%
!	ACAATGTTGCATTTTATGAG+CGG	+	chr5_2:12145601-12145620	Msa0757320:intron	30.0%
	ACTATAGGCTAAAGATTTAG+AGG	+	chr5_2:12145682-12145701	Msa0757320:intron	30.0%
	CGATCATAAATCATGTCTAT+AGG	-	chr5_2:12145719-12145738	None:intergenic	30.0%
!!	ATCACATTTTGAAGAAACTG+TGG	+	chr5_2:12145840-12145859	Msa0757320:intron	30.0%
	CTCACTAAAGCAAGTTTAAA+GGG	-	chr5_2:12145962-12145981	None:intergenic	30.0%
	TCTCACTAAAGCAAGTTTAA+AGG	-	chr5_2:12145963-12145982	None:intergenic	30.0%
	TAGGTTCTGTAAAATTTACC+AGG	-	chr5_2:12146049-12146068	None:intergenic	30.0%
!!	TTTTCAGGAAACAACGAAGA+TGG	+	chr5_2:12144278-12144297	Msa0757320:five_prime_UTR	35.0%
!!	TCGTTTTCTGTTGCATTGAT+TGG	+	chr5_2:12144424-12144443	Msa0757320:intron	35.0%
!!!	CGTTTTCTGTTGCATTGATT+GGG	+	chr5_2:12144425-12144444	Msa0757320:intron	35.0%
	AAGGAAAGCGAAATGATGAA+AGG	-	chr5_2:12144605-12144624	None:intergenic	35.0%
	AATTCTCTTTCCTTTCCACT+TGG	+	chr5_2:12144629-12144648	Msa0757320:intron	35.0%
	GTTGAAATTTCCAAGTGGAA+AGG	-	chr5_2:12144642-12144661	None:intergenic	35.0%
!	TTGGACGTGTGAACTTTTAT+TGG	-	chr5_2:12144712-12144731	None:intergenic	35.0%
	AGTTCACACGTCCAAAAATA+TGG	+	chr5_2:12144717-12144736	Msa0757320:intron	35.0%
!!!	GTCGCTAACTGTTTTAATTG+TGG	+	chr5_2:12144989-12145008	Msa0757320:intron	35.0%
!!!	GCTAACTGTTTTAATTGTGG+AGG	+	chr5_2:12144992-12145011	Msa0757320:intron	35.0%
!!!	ACTGTTTTAATTGTGGAGGA+TGG	+	chr5_2:12144996-12145015	Msa0757320:intron	35.0%
	TATATAGAGGGTAGTGAAAG+TGG	+	chr5_2:12145022-12145041	Msa0757320:intron	35.0%
	CTAGCGCTTGGTAAAAATAA+TGG	+	chr5_2:12145086-12145105	Msa0757320:intron	35.0%
	TCTTGGTATGATTGAACAGT+AGG	+	chr5_2:12145134-12145153	Msa0757320:intron	35.0%
	CTTGGTATGATTGAACAGTA+GGG	+	chr5_2:12145135-12145154	Msa0757320:intron	35.0%
	AATGATCTGACAATGAAGGA+TGG	-	chr5_2:12145200-12145219	None:intergenic	35.0%
!	TATCTGCTACTTCTCCTTTA+AGG	-	chr5_2:12145227-12145246	None:intergenic	35.0%
	GATTGAGGGAATGGAAAATT+TGG	-	chr5_2:12145348-12145367	None:intergenic	35.0%
	TATAAGCTAAAGCAAGTTCC+AGG	+	chr5_2:12145382-12145401	Msa0757320:intron	35.0%
	CATGTCTATAGGCAAACTAT+TGG	-	chr5_2:12145708-12145727	None:intergenic	35.0%
	AACAAAACCTTATTCCAGCA+GGG	-	chr5_2:12145780-12145799	None:intergenic	35.0%
!	CAAGAACTTTGATCTGAAAC+TGG	-	chr5_2:12146289-12146308	None:intergenic	35.0%
	TTTCAGATCAAAGTTCTTGC+TGG	+	chr5_2:12146290-12146309	Msa0757320:three_prime_UTR	35.0%
	GCACATGCTTCTTATAACTT+GGG	-	chr5_2:12146322-12146341	None:intergenic	35.0%
	ATACAACACTGTGACTCATT+TGG	-	chr5_2:12146344-12146363	None:intergenic	35.0%
	ATAGGTTTGTCAATAGGTAG+AGG	+	chr5_2:12146437-12146456	Msa0757320:three_prime_UTR	35.0%
	TAGGTTTGTCAATAGGTAGA+GGG	+	chr5_2:12146438-12146457	Msa0757320:three_prime_UTR	35.0%
!!	TTTTCAGGAAACAACGAAGA+TGG	+	chr5_2:12144278-12144297	Msa0757320:five_prime_UTR	35.0%
!!	TCGTTTTCTGTTGCATTGAT+TGG	+	chr5_2:12144424-12144443	Msa0757320:intron	35.0%
!!!	CGTTTTCTGTTGCATTGATT+GGG	+	chr5_2:12144425-12144444	Msa0757320:intron	35.0%
	AAGGAAAGCGAAATGATGAA+AGG	-	chr5_2:12144605-12144624	None:intergenic	35.0%
	AATTCTCTTTCCTTTCCACT+TGG	+	chr5_2:12144629-12144648	Msa0757320:intron	35.0%
	GTTGAAATTTCCAAGTGGAA+AGG	-	chr5_2:12144642-12144661	None:intergenic	35.0%
!	TTGGACGTGTGAACTTTTAT+TGG	-	chr5_2:12144712-12144731	None:intergenic	35.0%
	AGTTCACACGTCCAAAAATA+TGG	+	chr5_2:12144717-12144736	Msa0757320:intron	35.0%
!!!	GTCGCTAACTGTTTTAATTG+TGG	+	chr5_2:12144989-12145008	Msa0757320:intron	35.0%
!!!	GCTAACTGTTTTAATTGTGG+AGG	+	chr5_2:12144992-12145011	Msa0757320:intron	35.0%
!!!	ACTGTTTTAATTGTGGAGGA+TGG	+	chr5_2:12144996-12145015	Msa0757320:intron	35.0%
	TATATAGAGGGTAGTGAAAG+TGG	+	chr5_2:12145022-12145041	Msa0757320:intron	35.0%
	CTAGCGCTTGGTAAAAATAA+TGG	+	chr5_2:12145086-12145105	Msa0757320:intron	35.0%
	TCTTGGTATGATTGAACAGT+AGG	+	chr5_2:12145134-12145153	Msa0757320:intron	35.0%
	CTTGGTATGATTGAACAGTA+GGG	+	chr5_2:12145135-12145154	Msa0757320:intron	35.0%
	AATGATCTGACAATGAAGGA+TGG	-	chr5_2:12145200-12145219	None:intergenic	35.0%
!	TATCTGCTACTTCTCCTTTA+AGG	-	chr5_2:12145227-12145246	None:intergenic	35.0%
	GATTGAGGGAATGGAAAATT+TGG	-	chr5_2:12145348-12145367	None:intergenic	35.0%
	TATAAGCTAAAGCAAGTTCC+AGG	+	chr5_2:12145382-12145401	Msa0757320:intron	35.0%
	CATGTCTATAGGCAAACTAT+TGG	-	chr5_2:12145708-12145727	None:intergenic	35.0%
	AACAAAACCTTATTCCAGCA+GGG	-	chr5_2:12145780-12145799	None:intergenic	35.0%
!	CAAGAACTTTGATCTGAAAC+TGG	-	chr5_2:12146289-12146308	None:intergenic	35.0%
	TTTCAGATCAAAGTTCTTGC+TGG	+	chr5_2:12146290-12146309	Msa0757320:three_prime_UTR	35.0%
	GCACATGCTTCTTATAACTT+GGG	-	chr5_2:12146322-12146341	None:intergenic	35.0%
	ATACAACACTGTGACTCATT+TGG	-	chr5_2:12146344-12146363	None:intergenic	35.0%
	ATAGGTTTGTCAATAGGTAG+AGG	+	chr5_2:12146437-12146456	Msa0757320:three_prime_UTR	35.0%
	TAGGTTTGTCAATAGGTAGA+GGG	+	chr5_2:12146438-12146457	Msa0757320:three_prime_UTR	35.0%
	GCAAGGTTGAAATTTCCAAG+TGG	-	chr5_2:12144647-12144666	None:intergenic	40.0%
	CCTGTTTATTAAGCTACGCA+AGG	-	chr5_2:12144664-12144683	None:intergenic	40.0%
	CCTTGCGTAGCTTAATAAAC+AGG	+	chr5_2:12144661-12144680	Msa0757320:intron	40.0%
	GATACTACCCACATACCATA+TGG	+	chr5_2:12144782-12144801	Msa0757320:intron	40.0%
	TACCCACATACCATATGGAT+AGG	+	chr5_2:12144787-12144806	Msa0757320:intron	40.0%
	TGCCTATCCATATGGTATGT+GGG	-	chr5_2:12144792-12144811	None:intergenic	40.0%
	ATGCCTATCCATATGGTATG+TGG	-	chr5_2:12144793-12144812	None:intergenic	40.0%
	AATTGCAACAGCTCAGATGT+GGG	+	chr5_2:12144909-12144928	Msa0757320:intron	40.0%
	TGGAGGATGGTACTATATAG+AGG	+	chr5_2:12145009-12145028	Msa0757320:intron	40.0%
	GGAGGATGGTACTATATAGA+GGG	+	chr5_2:12145010-12145029	Msa0757320:intron	40.0%
!	TTTTACCAAGCGCTAGATCA+GGG	-	chr5_2:12145082-12145101	None:intergenic	40.0%
!	TTTTTACCAAGCGCTAGATC+AGG	-	chr5_2:12145083-12145102	None:intergenic	40.0%
	AGGAGAAGTAGCAGATATTG+TGG	+	chr5_2:12145230-12145249	Msa0757320:intron	40.0%
	GAAAGTAGAGATTGAGGGAA+TGG	-	chr5_2:12145357-12145376	None:intergenic	40.0%
	AAGTTCCAGGAATATACCCT+TGG	+	chr5_2:12145395-12145414	Msa0757320:intron	40.0%
	AGACATCATGAACTACCGAT+GGG	-	chr5_2:12145501-12145520	None:intergenic	40.0%
	TAGACATCATGAACTACCGA+TGG	-	chr5_2:12145502-12145521	None:intergenic	40.0%
	ACAAAACCTTATTCCAGCAG+GGG	-	chr5_2:12145779-12145798	None:intergenic	40.0%
	CAACAAAACCTTATTCCAGC+AGG	-	chr5_2:12145781-12145800	None:intergenic	40.0%
!	TACAGAACCTATGCTGACTA+TGG	+	chr5_2:12146058-12146077	Msa0757320:intron	40.0%
!	ACAGAACCTATGCTGACTAT+GGG	+	chr5_2:12146059-12146078	Msa0757320:intron	40.0%
	CAATTGTCTTTCCACATCAC+TGG	-	chr5_2:12146095-12146114	None:intergenic	40.0%
	CAGTGATGTGGAAAGACAAT+TGG	+	chr5_2:12146093-12146112	Msa0757320:CDS	40.0%
	TGGACAGTTGTAACAGAAGA+TGG	+	chr5_2:12146113-12146132	Msa0757320:CDS	40.0%
	TGTGTTCAAACTGAGCTGAA+AGG	-	chr5_2:12146142-12146161	None:intergenic	40.0%
!!	ACCCTTTTGATAACACCTGA+TGG	+	chr5_2:12146161-12146180	Msa0757320:CDS	40.0%
!	CCCATCAGGTGTTATCAAAA+GGG	-	chr5_2:12146165-12146184	None:intergenic	40.0%
!!	CCCTTTTGATAACACCTGAT+GGG	+	chr5_2:12146162-12146181	Msa0757320:CDS	40.0%
!!	CCTTTTGATAACACCTGATG+GGG	+	chr5_2:12146163-12146182	Msa0757320:CDS	40.0%
	TGCGAGCTATGCAAAGATAT+TGG	-	chr5_2:12146225-12146244	None:intergenic	40.0%
	GGCACATGCTTCTTATAACT+TGG	-	chr5_2:12146323-12146342	None:intergenic	40.0%
	TCAATAGGTAGAGGGAGATT+AGG	+	chr5_2:12146446-12146465	Msa0757320:three_prime_UTR	40.0%
	GCAAGGTTGAAATTTCCAAG+TGG	-	chr5_2:12144647-12144666	None:intergenic	40.0%
	CCTGTTTATTAAGCTACGCA+AGG	-	chr5_2:12144664-12144683	None:intergenic	40.0%
	CCTTGCGTAGCTTAATAAAC+AGG	+	chr5_2:12144661-12144680	Msa0757320:intron	40.0%
	GATACTACCCACATACCATA+TGG	+	chr5_2:12144782-12144801	Msa0757320:intron	40.0%
	TACCCACATACCATATGGAT+AGG	+	chr5_2:12144787-12144806	Msa0757320:intron	40.0%
	TGCCTATCCATATGGTATGT+GGG	-	chr5_2:12144792-12144811	None:intergenic	40.0%
	ATGCCTATCCATATGGTATG+TGG	-	chr5_2:12144793-12144812	None:intergenic	40.0%
	AATTGCAACAGCTCAGATGT+GGG	+	chr5_2:12144909-12144928	Msa0757320:intron	40.0%
	TGGAGGATGGTACTATATAG+AGG	+	chr5_2:12145009-12145028	Msa0757320:intron	40.0%
	GGAGGATGGTACTATATAGA+GGG	+	chr5_2:12145010-12145029	Msa0757320:intron	40.0%
!	TTTTACCAAGCGCTAGATCA+GGG	-	chr5_2:12145082-12145101	None:intergenic	40.0%
!	TTTTTACCAAGCGCTAGATC+AGG	-	chr5_2:12145083-12145102	None:intergenic	40.0%
	AGGAGAAGTAGCAGATATTG+TGG	+	chr5_2:12145230-12145249	Msa0757320:intron	40.0%
	GAAAGTAGAGATTGAGGGAA+TGG	-	chr5_2:12145357-12145376	None:intergenic	40.0%
	AAGTTCCAGGAATATACCCT+TGG	+	chr5_2:12145395-12145414	Msa0757320:intron	40.0%
	AGACATCATGAACTACCGAT+GGG	-	chr5_2:12145501-12145520	None:intergenic	40.0%
	TAGACATCATGAACTACCGA+TGG	-	chr5_2:12145502-12145521	None:intergenic	40.0%
	ACAAAACCTTATTCCAGCAG+GGG	-	chr5_2:12145779-12145798	None:intergenic	40.0%
	CAACAAAACCTTATTCCAGC+AGG	-	chr5_2:12145781-12145800	None:intergenic	40.0%
!	TACAGAACCTATGCTGACTA+TGG	+	chr5_2:12146058-12146077	Msa0757320:intron	40.0%
!	ACAGAACCTATGCTGACTAT+GGG	+	chr5_2:12146059-12146078	Msa0757320:intron	40.0%
	CAATTGTCTTTCCACATCAC+TGG	-	chr5_2:12146095-12146114	None:intergenic	40.0%
	CAGTGATGTGGAAAGACAAT+TGG	+	chr5_2:12146093-12146112	Msa0757320:CDS	40.0%
	TGGACAGTTGTAACAGAAGA+TGG	+	chr5_2:12146113-12146132	Msa0757320:CDS	40.0%
	TGTGTTCAAACTGAGCTGAA+AGG	-	chr5_2:12146142-12146161	None:intergenic	40.0%
!!	ACCCTTTTGATAACACCTGA+TGG	+	chr5_2:12146161-12146180	Msa0757320:CDS	40.0%
!	CCCATCAGGTGTTATCAAAA+GGG	-	chr5_2:12146165-12146184	None:intergenic	40.0%
!!	CCCTTTTGATAACACCTGAT+GGG	+	chr5_2:12146162-12146181	Msa0757320:CDS	40.0%
!!	CCTTTTGATAACACCTGATG+GGG	+	chr5_2:12146163-12146182	Msa0757320:CDS	40.0%
	TGCGAGCTATGCAAAGATAT+TGG	-	chr5_2:12146225-12146244	None:intergenic	40.0%
	GGCACATGCTTCTTATAACT+TGG	-	chr5_2:12146323-12146342	None:intergenic	40.0%
	TCAATAGGTAGAGGGAGATT+AGG	+	chr5_2:12146446-12146465	Msa0757320:three_prime_UTR	40.0%
!	TGGCAGTGTGGCCATATTTT+TGG	-	chr5_2:12144731-12144750	None:intergenic	45.0%
	CATGGCAATGCCTATCCATA+TGG	-	chr5_2:12144800-12144819	None:intergenic	45.0%
	GAATTGCAACAGCTCAGATG+TGG	+	chr5_2:12144908-12144927	Msa0757320:intron	45.0%
	ATTGCAACAGCTCAGATGTG+GGG	+	chr5_2:12144910-12144929	Msa0757320:intron	45.0%
	GTAGTGAAAGTGGAGTGGAA+TGG	+	chr5_2:12145032-12145051	Msa0757320:intron	45.0%
	GAAAGTGGAGTGGAATGGAA+TGG	+	chr5_2:12145037-12145056	Msa0757320:intron	45.0%
!	TGACACCAAGGGTATATTCC+TGG	-	chr5_2:12145403-12145422	None:intergenic	45.0%
	CAAAACCTTATTCCAGCAGG+GGG	-	chr5_2:12145778-12145797	None:intergenic	45.0%
!	CCCCATCAGGTGTTATCAAA+AGG	-	chr5_2:12146166-12146185	None:intergenic	45.0%
!	TGGCAGTGTGGCCATATTTT+TGG	-	chr5_2:12144731-12144750	None:intergenic	45.0%
	CATGGCAATGCCTATCCATA+TGG	-	chr5_2:12144800-12144819	None:intergenic	45.0%
	GAATTGCAACAGCTCAGATG+TGG	+	chr5_2:12144908-12144927	Msa0757320:intron	45.0%
	ATTGCAACAGCTCAGATGTG+GGG	+	chr5_2:12144910-12144929	Msa0757320:intron	45.0%
	GTAGTGAAAGTGGAGTGGAA+TGG	+	chr5_2:12145032-12145051	Msa0757320:intron	45.0%
	GAAAGTGGAGTGGAATGGAA+TGG	+	chr5_2:12145037-12145056	Msa0757320:intron	45.0%
!	TGACACCAAGGGTATATTCC+TGG	-	chr5_2:12145403-12145422	None:intergenic	45.0%
	CAAAACCTTATTCCAGCAGG+GGG	-	chr5_2:12145778-12145797	None:intergenic	45.0%
!	CCCCATCAGGTGTTATCAAA+AGG	-	chr5_2:12146166-12146185	None:intergenic	45.0%
	AACAGCTCAGATGTGGGGAA+GGG	+	chr5_2:12144915-12144934	Msa0757320:intron	50.0%
	AGAGGGTAGTGAAAGTGGAG+TGG	+	chr5_2:12145027-12145046	Msa0757320:intron	50.0%
!	ATATAGCTGATTCCCCCTGC+TGG	+	chr5_2:12145763-12145782	Msa0757320:intron	50.0%
	TGATTCCCCCTGCTGGAATA+AGG	+	chr5_2:12145770-12145789	Msa0757320:intron	50.0%
	ATGCTGCCCATAGTCAGCAT+AGG	-	chr5_2:12146068-12146087	None:intergenic	50.0%
	GCAGCATCAATCCAGTGATG+TGG	+	chr5_2:12146081-12146100	Msa0757320:CDS	50.0%
	GTCATAATCTCAGCCCCATC+AGG	-	chr5_2:12146179-12146198	None:intergenic	50.0%
	AACAGCTCAGATGTGGGGAA+GGG	+	chr5_2:12144915-12144934	Msa0757320:intron	50.0%
	AGAGGGTAGTGAAAGTGGAG+TGG	+	chr5_2:12145027-12145046	Msa0757320:intron	50.0%
!	ATATAGCTGATTCCCCCTGC+TGG	+	chr5_2:12145763-12145782	Msa0757320:intron	50.0%
	TGATTCCCCCTGCTGGAATA+AGG	+	chr5_2:12145770-12145789	Msa0757320:intron	50.0%
	ATGCTGCCCATAGTCAGCAT+AGG	-	chr5_2:12146068-12146087	None:intergenic	50.0%
	GCAGCATCAATCCAGTGATG+TGG	+	chr5_2:12146081-12146100	Msa0757320:CDS	50.0%
	GTCATAATCTCAGCCCCATC+AGG	-	chr5_2:12146179-12146198	None:intergenic	50.0%
	CAACAGCTCAGATGTGGGGA+AGG	+	chr5_2:12144914-12144933	Msa0757320:intron	55.0%
	ACAGCTCAGATGTGGGGAAG+GGG	+	chr5_2:12144916-12144935	Msa0757320:intron	55.0%
	TGACTCCCTGATCTAGCGCT+TGG	+	chr5_2:12145074-12145093	Msa0757320:intron	55.0%
	CAACAGCTCAGATGTGGGGA+AGG	+	chr5_2:12144914-12144933	Msa0757320:intron	55.0%
	ACAGCTCAGATGTGGGGAAG+GGG	+	chr5_2:12144916-12144935	Msa0757320:intron	55.0%
	TGACTCCCTGATCTAGCGCT+TGG	+	chr5_2:12145074-12145093	Msa0757320:intron	55.0%
!	AGATGTGGGGAAGGGGTGCA+TGG	+	chr5_2:12144923-12144942	Msa0757320:intron	60.0%
!	GATGTGGGGAAGGGGTGCAT+GGG	+	chr5_2:12144924-12144943	Msa0757320:intron	60.0%
!	AGATGTGGGGAAGGGGTGCA+TGG	+	chr5_2:12144923-12144942	Msa0757320:intron	60.0%
!	GATGTGGGGAAGGGGTGCAT+GGG	+	chr5_2:12144924-12144943	Msa0757320:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	12144248	12146564	12144248	ID=Msa0757320;Name=Msa0757320
chr5_2	mRNA	12144248	12146564	12144248	ID=Msa0757320-mRNA-1;Parent=Msa0757320;Name=Msa0757320-mRNA-1;_AED=0.00;_eAED=0.00;_QI=57\|1\|1\|1\|0\|1\|2\|356\|57
chr5_2	exon	12144248	12144332	12144248	ID=Msa0757320-mRNA-1:exon:5375;Parent=Msa0757320-mRNA-1
chr5_2	exon	12146063	12146564	12146063	ID=Msa0757320-mRNA-1:exon:5376;Parent=Msa0757320-mRNA-1
chr5_2	five_prime_UTR	12144248	12144304	12144248	ID=Msa0757320-mRNA-1:five_prime_utr;Parent=Msa0757320-mRNA-1
chr5_2	CDS	12144305	12144332	12144305	ID=Msa0757320-mRNA-1:cds;Parent=Msa0757320-mRNA-1
chr5_2	CDS	12146063	12146208	12146063	ID=Msa0757320-mRNA-1:cds;Parent=Msa0757320-mRNA-1
chr5_2	three_prime_UTR	12146209	12146564	12146209	ID=Msa0757320-mRNA-1:three_prime_utr;Parent=Msa0757320-mRNA-1

Gene Sequence	>Msa0757320 ATGATGTTAAATCAAACCTTCACGATTGAACCTATGCTGACTATGGGCAGCATCAATCCAGTGATGTGGAAAGACAATTGGACAGTTGTAACAGAAGATGGAAGCCTTTCAGCTCAGTTTGAACACACCCTTTTGATAACACCTGATGGGGCTGAGATTATGACTCAATGCTGA
Protein sequence	>Msa0757320 MMLNQTFTIEPMLTMGSINPVMWKDNWTVVTEDGSLSAQFEHTLLITPDGAEIMTQC*