AlfalfaGEDB | Msa0532300

M. sativa cultivar zhongmu-4 / Msa0532300

KEGG: -
GO: -
PFAM: PF02338.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0532300	XP_003604582.1	98.496	133	2	0	1	133	97	229	4.98e-91	273

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0532300	sp\|Q8GYW0\|OTU3_ARATH	67.391	138	39	2	1	132	96	233	1.13e-57	181

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0532300	G7JLU6	98.496	133	2	0	1	133	97	229	2.38e-91	273

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001950	Msa0532300	0.811431	7.024395e-51	-8.615850e-47
Msa0008850	Msa0532300	0.809337	1.989046e-50	-8.615850e-47
Msa0011720	Msa0532300	0.820117	8.127123e-53	-8.615850e-47
Msa0017120	Msa0532300	0.820587	6.339301e-53	-8.615850e-47
Msa0017940	Msa0532300	0.801759	7.737861e-49	-8.615850e-47
Msa0020630	Msa0532300	0.835135	2.000840e-56	-8.615850e-47
Msa0022640	Msa0532300	0.832909	7.223235e-56	-8.615850e-47
Msa0024680	Msa0532300	0.858989	5.637938e-63	-8.615850e-47
Msa0027540	Msa0532300	0.872917	2.200211e-67	-8.615850e-47
Msa0028060	Msa0532300	0.811836	5.735243e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0532300	MtrunA17_Chr4g0005721	98.496	133	2	0	1	133	97	229	4.58e-95	273
Msa0532300	MtrunA17_Chr1g0174241	34.400	125	65	4	8	132	218	325	3.93e-12	62.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0532300	AT2G38025.1	67.391	138	39	2	1	132	96	233	1.15e-58	181
Msa0532300	AT2G38025.2	67.391	138	39	2	1	132	73	210	2.41e-58	180
Msa0532300	AT3G57810.2	33.071	127	68	3	7	133	195	304	2.34e-14	68.6
Msa0532300	AT3G57810.4	33.071	127	68	3	7	133	195	304	2.34e-14	68.6
Msa0532300	AT3G57810.3	33.071	127	68	3	7	133	195	304	2.34e-14	68.6

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Find 57 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCAGCATAGAGGCAG+TGG	0.587658	4_1:-6318992	Msa0532300:intron
AAACTAACTTATAATAATTT+GGG	0.337402	4_1:+6318662	None:intergenic
AATGGCTCTTAACCAACGCG+AGG	0.698050	4_1:-6320011	Msa0532300:CDS
AATTGGGGTAATAGTCATTT+TGG	0.177304	4_1:-6318629	Msa0532300:three_prime_UTR
ACATTAATTACCTTCCCATT+AGG	0.378095	4_1:-6318723	Msa0532300:three_prime_UTR
AGAAGCCATCATTGCCGTCA+CGG	0.595204	4_1:-6319658	Msa0532300:CDS
AGAGGCAGTGGTCGGGGATC+TGG	0.401931	4_1:-6318980	Msa0532300:CDS
AGGAATTGTATCGGCGAAGG+AGG	0.586453	4_1:-6318852	Msa0532300:three_prime_UTR
AGTTTGATAATTTATAATTG+GGG	0.550558	4_1:-6318644	Msa0532300:three_prime_UTR
ATACAATTCCTCATAACAGA+AGG	0.623406	4_1:+6318864	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTGAAAGCAAAACAAACAAA+AGG	+	chr4_1:6318493-6318512	None:intergenic	30.0%
GGAAATGAAAACAAAGTGAA+TGG	+	chr4_1:6318647-6318666	None:intergenic	30.0%
GAAGCTATATGTGAAAATGT+AGG	-	chr4_1:6318724-6318743	Msa0532300:three_prime_UTR	30.0%
AGAAACTCTATTTAGTCCTT+AGG	+	chr4_1:6319004-6319023	None:intergenic	30.0%
TCTGGTATGTACACAATAAT+TGG	+	chr4_1:6319293-6319312	None:intergenic	30.0%
AAACTAAAAATGCAGCATAG+AGG	-	chr4_1:6319425-6319444	Msa0532300:intron	30.0%
ATACAATTCCTCATAACAGA+AGG	+	chr4_1:6319562-6319581	None:intergenic	30.0%
TCTGTTATGAGGAATTGTAT+CGG	-	chr4_1:6319562-6319581	Msa0532300:intron	30.0%
ACATTAATTACCTTCCCATT+AGG	-	chr4_1:6319700-6319719	Msa0532300:CDS	30.0%
GATTCATTGATGGAATATTG+CGG	+	chr4_1:6319842-6319861	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	6318393	6320052	6318393	ID=Msa0532300;Name=Msa0532300
chr4_1	mRNA	6318393	6320052	6318393	ID=Msa0532300-mRNA-1;Parent=Msa0532300;Name=Msa0532300-mRNA-1;_AED=0.21;_eAED=0.21;_QI=2\|1\|1\|1\|0.8\|0.66\|6\|453\|133
chr4_1	exon	6318393	6318450	6318393	ID=Msa0532300-mRNA-1:exon:2274;Parent=Msa0532300-mRNA-1
chr4_1	exon	6318479	6319005	6318479	ID=Msa0532300-mRNA-1:exon:2273;Parent=Msa0532300-mRNA-1
chr4_1	exon	6319126	6319179	6319126	ID=Msa0532300-mRNA-1:exon:2272;Parent=Msa0532300-mRNA-1
chr4_1	exon	6319489	6319543	6319489	ID=Msa0532300-mRNA-1:exon:2271;Parent=Msa0532300-mRNA-1
chr4_1	exon	6319639	6319744	6319639	ID=Msa0532300-mRNA-1:exon:2270;Parent=Msa0532300-mRNA-1
chr4_1	exon	6319996	6320052	6319996	ID=Msa0532300-mRNA-1:exon:2269;Parent=Msa0532300-mRNA-1
chr4_1	five_prime_UTR	6320051	6320052	6320051	ID=Msa0532300-mRNA-1:five_prime_utr;Parent=Msa0532300-mRNA-1
chr4_1	CDS	6319996	6320050	6319996	ID=Msa0532300-mRNA-1:cds;Parent=Msa0532300-mRNA-1
chr4_1	CDS	6319639	6319744	6319639	ID=Msa0532300-mRNA-1:cds;Parent=Msa0532300-mRNA-1
chr4_1	CDS	6319489	6319543	6319489	ID=Msa0532300-mRNA-1:cds;Parent=Msa0532300-mRNA-1
chr4_1	CDS	6319126	6319179	6319126	ID=Msa0532300-mRNA-1:cds;Parent=Msa0532300-mRNA-1
chr4_1	CDS	6318874	6319005	6318874	ID=Msa0532300-mRNA-1:cds;Parent=Msa0532300-mRNA-1
chr4_1	three_prime_UTR	6318479	6318873	6318479	ID=Msa0532300-mRNA-1:three_prime_utr;Parent=Msa0532300-mRNA-1
chr4_1	three_prime_UTR	6318393	6318450	6318393	ID=Msa0532300-mRNA-1:three_prime_utr;Parent=Msa0532300-mRNA-1

Gene Sequence

>Msa0532300

ATGGCTCACAACAAGGGAATGGCTCTTAACCAACGCGAGGAGAGAGAAAATGCAGATGAATTAAGAATGGCAGTCAAAGAAGCTATATGTGAAAATGTAGGAGATCGCAAGTTATATGAAGAAGCCATCATTGCCGTCACGGTTGATGAGCCTTTACAACGTTACTGCAGGCGGATTGGGCAACCTGATTTCTGGGGAGGAGAATCAGAACTATTGGTACTGTCAAAGTTATGTAAGCAGCCAATTATTGTGTACATACCAGAGCATGAGCATAGAGGCAGTGGTCGGGGATCTGGTTTCATTCCGATTGCCGAGTATGGAAGTGAATTTAAAAAGGGTTCTAGCAGAAAAGCTGTGAGGCTATTGTTCAGCGGTAAAAACCATTATGACCTTCTGTTATGA

Protein sequence

>Msa0532300

MAHNKGMALNQREERENADELRMAVKEAICENVGDRKLYEEAIIAVTVDEPLQRYCRRIGQPDFWGGESELLVLSKLCKQPIIVYIPEHEHRGSGRGSGFIPIAEYGSEFKKGSSRKAVRLLFSGKNHYDLLL*