AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0834260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0834260 MtrunA17_Chr5g0399351 97.159 352 9 1 9 360 1 351 0.0 673
Msa0834260 MtrunA17_Chr2g0317181 85.227 88 13 0 229 316 4 91 8.42e-43 144
Msa0834260 MtrunA17_Chr2g0317191 63.636 99 34 2 12 108 5 103 2.51e-28 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0834260 AT5G05210.1 49.459 370 132 8 31 356 24 382 2.54e-92 281
Msa0834260 AT5G05210.2 49.459 370 132 8 31 356 16 374 3.13e-92 281
Msa0834260 AT2G27750.1 51.667 180 66 4 172 347 19 181 6.07e-45 152

Find 94 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTAACCCATGTTATATTT+TGG 0.153792 5_4:+7270834 Msa0834260:three_prime_UTR
TCACTAGAATACAGGCATTT+TGG 0.215580 5_4:+7270893 Msa0834260:three_prime_UTR
TAACGAGAAACCGTGGTTTC+AGG 0.232330 5_4:+7269793 Msa0834260:CDS
GCCTGTATTCTAGTGATAAA+TGG 0.241539 5_4:-7270886 None:intergenic
TGAACTTCTCAAGAAGAGTT+TGG 0.258601 5_4:+7269919 Msa0834260:CDS
AGAAGTTCAAGTGTGGTTAC+AGG 0.290349 5_4:-7269905 None:intergenic
AACTCTCTCTTCCCATTTCT+CGG 0.303045 5_4:-7270521 None:intergenic
TATCATCATGGCAGATTTAC+CGG 0.336518 5_4:+7270956 Msa0834260:three_prime_UTR
GCAGCAATGGACAGAGCTTC+AGG 0.337717 5_4:+7270424 Msa0834260:CDS
AGTGTGGTTACAGGCTTCTC+AGG 0.342543 5_4:-7269896 None:intergenic
GTTTGGTCACGTTAAACTTC+AGG 0.346929 5_4:+7270270 Msa0834260:CDS
GAACTTCTCAAGAAGAGTTT+GGG 0.346980 5_4:+7269920 Msa0834260:CDS
AGGTGCGGGCTTACTTTCAT+TGG 0.357636 5_4:-7270188 None:intergenic
CAAGCTTGCGATGAAGCCTT+TGG 0.361638 5_4:-7270045 None:intergenic
TCCATTTATCACTAGAATAC+AGG 0.366047 5_4:+7270885 Msa0834260:three_prime_UTR
ACAGAATATCAAGAAGTCTA+AGG 0.367209 5_4:+7269859 Msa0834260:CDS
CTATTGCGGCCGGGCTTTGA+AGG 0.371671 5_4:+7270652 Msa0834260:CDS
TTCTCTTGTAGCTTCTCATC+AGG 0.381853 5_4:-7270208 None:intergenic
CAAGAGAGGATATCATTATC+AGG 0.385515 5_4:+7270138 Msa0834260:CDS
AGTTGTTAATTACATATTGT+TGG 0.390747 5_4:+7270924 Msa0834260:three_prime_UTR
GGACAGAGCTTCAGGAATTA+AGG 0.391083 5_4:+7270432 Msa0834260:CDS
TGTTGATGTTGGTCAAGATT+TGG 0.391866 5_4:+7269688 Msa0834260:CDS
GATAAAGAAAAGCATACATA+AGG 0.405087 5_4:+7270474 Msa0834260:CDS
ATAAAGAGGAGACATGAAAA+TGG 0.409760 5_4:+7267983 None:intergenic
AAGCCACACGTTGAAATTGA+TGG 0.412512 5_4:-7270773 None:intergenic
CCTCCGATAACAATGCAAAA+AGG 0.413586 5_4:-7270739 None:intergenic
TAATCTAATGGTTAATGAAC+CGG 0.420372 5_4:-7270975 None:intergenic
GCTTCAAAGGAGCTTGTGTT+TGG 0.427667 5_4:+7270253 Msa0834260:CDS
GCAATAGCTTCACCCTTCTC+AGG 0.428203 5_4:-7270382 None:intergenic
AACTCGTAACTGTGCAGATC+CGG 0.428390 5_4:+7270081 Msa0834260:CDS
GGCCGGGCTTTGAAGGTCAC+AGG 0.430067 5_4:+7270659 Msa0834260:CDS
CCTTTGGCGAAGCTCTTCAT+AGG 0.445722 5_4:-7270029 None:intergenic
AACGAGAAACCGTGGTTTCA+GGG 0.456991 5_4:+7269794 Msa0834260:CDS
ATAAAGAAAAGCATACATAA+GGG 0.460274 5_4:+7270475 Msa0834260:CDS
GGGTGAAGCTATTGCTAAGA+AGG 0.461254 5_4:+7270390 Msa0834260:CDS
ATGCTGTTATAGGGAGACTT+TGG 0.465023 5_4:+7269626 Msa0834260:intron
ATACAAATAACATAATCTAA+TGG 0.473511 5_4:-7270987 None:intergenic
GGATGCCGCGGAGGCTTCAA+AGG 0.475053 5_4:+7270240 Msa0834260:CDS
GTTGGCAGATAAAACTTAGC+TGG 0.478956 5_4:-7269764 None:intergenic
AGGCTTCATCGCAAGCTTGA+AGG 0.479052 5_4:+7270049 Msa0834260:CDS
AAGTGAGGAAGAAGAGGATG+TGG 0.481324 5_4:+7269961 Msa0834260:CDS
TGTAGCTTCTCATCAGGTGC+GGG 0.484265 5_4:-7270202 None:intergenic
GTTGCAGAGGTTGTTGATGT+TGG 0.484416 5_4:+7269677 Msa0834260:CDS
CTGATAATGATATCCTCTCT+TGG 0.486659 5_4:-7270137 None:intergenic
GGGAAGAGAGAGTTCAATCA+AGG 0.493714 5_4:+7270530 Msa0834260:CDS
GCTACAAGAGAAGGTGAAGA+AGG 0.500602 5_4:+7270219 Msa0834260:CDS
ACTTGAAAGGGCTAAGAAGT+TGG 0.501671 5_4:+7270339 Msa0834260:CDS
CGGAAAAGGCTGCTAAGAAG+AGG 0.504510 5_4:+7270101 Msa0834260:CDS
CCATCCAGCTCAGACACAAA+AGG 0.508756 5_4:-7269989 None:intergenic
GATGAAGATGAGGAAGCAAA+AGG 0.510760 5_4:+7269652 Msa0834260:CDS
AAAGCACAAGGAACTTGAAA+GGG 0.512350 5_4:+7270327 Msa0834260:CDS
TAACTGTGCAGATCCGGAAA+AGG 0.514146 5_4:+7270087 Msa0834260:CDS
TCAATCAAGGGATCAGTTGA+AGG 0.518168 5_4:+7270543 Msa0834260:CDS
GGCTTTGAAGGTCACAGGGA+AGG 0.530107 5_4:+7270664 Msa0834260:CDS
AGGCAGAGAAGCAAAAGAAA+AGG 0.532776 5_4:+7270563 Msa0834260:CDS
ACTTTGGAGTGATGAAGATG+AGG 0.541362 5_4:+7269642 Msa0834260:CDS
CAAAGCACAAGGAACTTGAA+AGG 0.542564 5_4:+7270326 Msa0834260:CDS
TCACGTTAAACTTCAGGATG+AGG 0.542978 5_4:+7270276 Msa0834260:CDS
GAATGAAAGTGAGGAAGAAG+AGG 0.544065 5_4:+7269955 Msa0834260:CDS
GGAAGCATGGAAAGCAGCAA+TGG 0.549880 5_4:+7270411 Msa0834260:CDS
TTATCAATTTGGGATCGTCA+TGG 0.557381 5_4:-7270456 None:intergenic
GGATTAATGATAAGAAAATG+AGG 0.557444 5_4:+7270605 Msa0834260:CDS
GGTTTCTCGTTATCATCAGT+TGG 0.560730 5_4:-7269782 None:intergenic
TGGTTTGCATCATATCATCA+TGG 0.561936 5_4:+7270944 Msa0834260:three_prime_UTR
GAAGAAGAATGATCCTGAGA+AGG 0.567162 5_4:+7270369 Msa0834260:CDS
CTTCAGGATGAGGAAATGCT+AGG 0.569079 5_4:+7270286 Msa0834260:CDS
TCCCTGTGACCTTCAAAGCC+CGG 0.571851 5_4:-7270661 None:intergenic
TGAAAGGGCTAAGAAGTTGG+AGG 0.572734 5_4:+7270342 Msa0834260:CDS
TTGTAGCTTCTCATCAGGTG+CGG 0.577217 5_4:-7270203 None:intergenic
TTGTTGAGACCCTGAAACCA+CGG 0.583522 5_4:-7269803 None:intergenic
TGATGAGAAGCTACAAGAGA+AGG 0.587083 5_4:+7270210 Msa0834260:CDS
GTGTGGTTACAGGCTTCTCA+GGG 0.588570 5_4:-7269895 None:intergenic
CATCCATCAATTTCAACGTG+TGG 0.589707 5_4:+7270770 Msa0834260:three_prime_UTR
GAAGCAAAAGGTTGTTGCAG+AGG 0.592741 5_4:+7269664 Msa0834260:CDS
ATCAGGATAATAAACGCAAG+AGG 0.601271 5_4:+7270155 Msa0834260:CDS
CTGAGCTGGATGGAGATGAT+CGG 0.601585 5_4:+7269999 Msa0834260:CDS
AAGAAGAATGATCCTGAGAA+GGG 0.603235 5_4:+7270370 Msa0834260:CDS
CTATTGCTAAGAAGGAAGCA+TGG 0.605991 5_4:+7270398 Msa0834260:CDS
GTAATTGCCAAAATATAACA+TGG 0.609450 5_4:-7270841 None:intergenic
GAAGGTGAAGAAGGATGCCG+CGG 0.613578 5_4:+7270228 Msa0834260:CDS
CTTCTTGAGAAGTTCAAGTG+TGG 0.616015 5_4:-7269912 None:intergenic
TAAAGAAAAGCATACATAAG+GGG 0.624269 5_4:+7270476 Msa0834260:CDS
TCAGGATAATAAACGCAAGA+GGG 0.627512 5_4:+7270156 Msa0834260:CDS
GGAAGAGAGAGTTCAATCAA+GGG 0.629511 5_4:+7270531 Msa0834260:CDS
CAACAAGAAACAGAAAGCAA+AGG 0.638546 5_4:+7269820 Msa0834260:CDS
GGAAAAGGCTGCTAAGAAGA+GGG 0.647760 5_4:+7270102 Msa0834260:CDS
GCCGGGCTTTGAAGGTCACA+GGG 0.648501 5_4:+7270660 Msa0834260:CDS
GGTGAAGAAGGATGCCGCGG+AGG 0.652339 5_4:+7270231 Msa0834260:CDS
CCTATGAAGAGCTTCGCCAA+AGG 0.661576 5_4:+7270029 Msa0834260:CDS
TAATTGCCAAAATATAACAT+GGG 0.683860 5_4:-7270840 None:intergenic
GAAGATAGAGACACCAAGAG+AGG 0.685788 5_4:+7270124 Msa0834260:CDS
AAGCTCCTTTGAAGCCTCCG+CGG 0.690454 5_4:-7270245 None:intergenic
GGTCAGATAATATAGCTGAG+AGG 0.714827 5_4:+7270584 Msa0834260:CDS
CTGATGATAACGAGAAACCG+TGG 0.715203 5_4:+7269786 Msa0834260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATTTTAAAAAAATAAAC+CGG + chr5_4:7268685-7268704 Msa0834260:intron 10.0%
!!! TTTCTTTTTTAATTGGTTTT+AGG + chr5_4:7269028-7269047 Msa0834260:intron 15.0%
!!! TTCTTTTTTAATTGGTTTTA+GGG + chr5_4:7269029-7269048 Msa0834260:intron 15.0%
!!! ATTTATTTTTTTCTTCAGTA+TGG - chr5_4:7269441-7269460 None:intergenic 15.0%
!! ATACAAATAACATAATCTAA+TGG - chr5_4:7270990-7271009 None:intergenic 15.0%
!! TTACAAAAAAAAATCTCCTT+CGG - chr5_4:7268125-7268144 None:intergenic 20.0%
!!! TTTTGTAAATTGCTTTTTCT+CGG + chr5_4:7268137-7268156 Msa0834260:intron 20.0%
!! TTAATTTCTCTAAGTTTGAT+GGG - chr5_4:7268630-7268649 None:intergenic 20.0%
!!! TTTAATTTCTCTAAGTTTGA+TGG - chr5_4:7268631-7268650 None:intergenic 20.0%
!!! GCAAATGTTTCTTTTTTAAT+TGG + chr5_4:7269021-7269040 Msa0834260:intron 20.0%
!! TAATACTAGTTATAGAGTTA+GGG - chr5_4:7269315-7269334 None:intergenic 20.0%
!!! ATGCTTTTCTTTATCAATTT+GGG - chr5_4:7270469-7270488 None:intergenic 20.0%
!!! TATGCTTTTCTTTATCAATT+TGG - chr5_4:7270470-7270489 None:intergenic 20.0%
!!! ATAAAGAAAAGCATACATAA+GGG + chr5_4:7270475-7270494 Msa0834260:CDS 20.0%
!!! TATTAACCCATGTTATATTT+TGG + chr5_4:7270834-7270853 Msa0834260:three_prime_UTR 20.0%
!! TAATTGCCAAAATATAACAT+GGG - chr5_4:7270843-7270862 None:intergenic 20.0%
!! AATGGAAATAGTCAAAAAAA+AGG - chr5_4:7270871-7270890 None:intergenic 20.0%
!! AGTTGTTAATTACATATTGT+TGG + chr5_4:7270924-7270943 Msa0834260:three_prime_UTR 20.0%
!! AGGTTAAACAATTGATTTCA+TGG + chr5_4:7268348-7268367 Msa0834260:intron 25.0%
!! GGTTAAACAATTGATTTCAT+GGG + chr5_4:7268349-7268368 Msa0834260:intron 25.0%
! TGATTGGGATTTAGAAATTA+GGG - chr5_4:7268781-7268800 None:intergenic 25.0%
! AACCTTATACATAACATAAG+CGG - chr5_4:7269093-7269112 None:intergenic 25.0%
! GTAATACTAGTTATAGAGTT+AGG - chr5_4:7269316-7269335 None:intergenic 25.0%
! AGTATTACTATACATATTGC+TGG + chr5_4:7269328-7269347 Msa0834260:intron 25.0%
!! TCTGTGTAATTTTCAAATTC+TGG + chr5_4:7269511-7269530 Msa0834260:intron 25.0%
!! GATAAAGAAAAGCATACATA+AGG + chr5_4:7270474-7270493 Msa0834260:CDS 25.0%
!! TAAAGAAAAGCATACATAAG+GGG + chr5_4:7270476-7270495 Msa0834260:CDS 25.0%
! GGATTAATGATAAGAAAATG+AGG + chr5_4:7270605-7270624 Msa0834260:CDS 25.0%
! GTAATTGCCAAAATATAACA+TGG - chr5_4:7270844-7270863 None:intergenic 25.0%
! TAATCTAATGGTTAATGAAC+CGG - chr5_4:7270978-7270997 None:intergenic 25.0%
TGGTGTTTGTTCAACAAAAA+TGG + chr5_4:7268003-7268022 Msa0834260:intron 30.0%
!!! ACAAAAATGGTGTTTTGAAG+AGG + chr5_4:7268016-7268035 Msa0834260:intron 30.0%
! AAAAGTATGAAACAACTCCT+CGG + chr5_4:7268089-7268108 Msa0834260:intron 30.0%
TGTTGTTGACACATTAGAAA+AGG - chr5_4:7268270-7268289 None:intergenic 30.0%
AATCTCCTCTTGAAACAAAT+AGG - chr5_4:7268379-7268398 None:intergenic 30.0%
! CGTATTTTACTTGTGTCAAT+AGG + chr5_4:7268545-7268564 Msa0834260:intron 30.0%
ATAGTGTGAGAGACAATAAT+AGG - chr5_4:7268712-7268731 None:intergenic 30.0%
GTGATTGGGATTTAGAAATT+AGG - chr5_4:7268782-7268801 None:intergenic 30.0%
GAAGAAAATAAAGAACGAAG+TGG - chr5_4:7268942-7268961 None:intergenic 30.0%
AACCGCTTATGTTATGTATA+AGG + chr5_4:7269088-7269107 Msa0834260:intron 30.0%
ATGTATGCTATAAGCTAGAT+TGG + chr5_4:7269163-7269182 Msa0834260:intron 30.0%
TTAAATGTAATCACATGTGC+TGG + chr5_4:7269464-7269483 Msa0834260:intron 30.0%
TTATTCTCTCATGCTGTTAT+AGG + chr5_4:7269616-7269635 Msa0834260:intron 30.0%
TATTCTCTCATGCTGTTATA+GGG + chr5_4:7269617-7269636 Msa0834260:intron 30.0%
AACCAATTTGTCGAAAAAAC+TGG - chr5_4:7269735-7269754 None:intergenic 30.0%
ACAGAATATCAAGAAGTCTA+AGG + chr5_4:7269859-7269878 Msa0834260:CDS 30.0%
GAGAAAAATTTCAAAGCACA+AGG + chr5_4:7270315-7270334 Msa0834260:CDS 30.0%
AAGAAAAAAATGCCGAGAAA+TGG + chr5_4:7270509-7270528 Msa0834260:CDS 30.0%
AGAAAAAAATGCCGAGAAAT+GGG + chr5_4:7270510-7270529 Msa0834260:CDS 30.0%
!!! GGTTTTATTAATAGCAGTTC+TGG + chr5_4:7270685-7270704 Msa0834260:CDS 30.0%
! GTACCTTTTTGCATTGTTAT+CGG + chr5_4:7270736-7270755 Msa0834260:three_prime_UTR 30.0%
TCCATTTATCACTAGAATAC+AGG + chr5_4:7270885-7270904 Msa0834260:three_prime_UTR 30.0%
! AAAATACGTTTTTACCCCCT+CGG - chr5_4:7268232-7268251 None:intergenic 35.0%
GAACAAGATGAAGTTGTTGA+TGG + chr5_4:7268310-7268329 Msa0834260:intron 35.0%
GTAGACCTATTTGTTTCAAG+AGG + chr5_4:7268371-7268390 Msa0834260:intron 35.0%
TTGTTTCAAGAGGAGATTAG+AGG + chr5_4:7268381-7268400 Msa0834260:intron 35.0%
TGTATTGTTGTGACCTTTGA+TGG + chr5_4:7268460-7268479 Msa0834260:intron 35.0%
AGCTGTAGTTAACAACTGTT+GGG + chr5_4:7268516-7268535 Msa0834260:intron 35.0%
AATAACGAGACCATACCTTT+GGG - chr5_4:7268856-7268875 None:intergenic 35.0%
AAATAACGAGACCATACCTT+TGG - chr5_4:7268857-7268876 None:intergenic 35.0%
!!! AATTGGTTTTAGGGTCTGTT+TGG + chr5_4:7269038-7269057 Msa0834260:intron 35.0%
! TAAGCTTTGTGAGACTGTTT+GGG - chr5_4:7269254-7269273 None:intergenic 35.0%
! ATAAGCTTTGTGAGACTGTT+TGG - chr5_4:7269255-7269274 None:intergenic 35.0%
TTATAGAGTTAGGGACTGTT+TGG - chr5_4:7269306-7269325 None:intergenic 35.0%
ACTATACATATTGCTGGCAA+AGG + chr5_4:7269334-7269353 Msa0834260:intron 35.0%
TGTTGATGTTGGTCAAGATT+TGG + chr5_4:7269688-7269707 Msa0834260:CDS 35.0%
!!! TGCCAGTTTTTTCGACAAAT+TGG + chr5_4:7269730-7269749 Msa0834260:CDS 35.0%
CAACAAGAAACAGAAAGCAA+AGG + chr5_4:7269820-7269839 Msa0834260:CDS 35.0%
TGAACTTCTCAAGAAGAGTT+TGG + chr5_4:7269919-7269938 Msa0834260:CDS 35.0%
GAACTTCTCAAGAAGAGTTT+GGG + chr5_4:7269920-7269939 Msa0834260:CDS 35.0%
AGAAAAAGCGAATGAAAGTG+AGG + chr5_4:7269946-7269965 Msa0834260:CDS 35.0%
! CTGATAATGATATCCTCTCT+TGG - chr5_4:7270140-7270159 None:intergenic 35.0%
CAAGAGAGGATATCATTATC+AGG + chr5_4:7270138-7270157 Msa0834260:CDS 35.0%
ATCAGGATAATAAACGCAAG+AGG + chr5_4:7270155-7270174 Msa0834260:CDS 35.0%
TCAGGATAATAAACGCAAGA+GGG + chr5_4:7270156-7270175 Msa0834260:CDS 35.0%
AAAGCACAAGGAACTTGAAA+GGG + chr5_4:7270327-7270346 Msa0834260:CDS 35.0%
AAGAAGAATGATCCTGAGAA+GGG + chr5_4:7270370-7270389 Msa0834260:CDS 35.0%
! TTATCAATTTGGGATCGTCA+TGG - chr5_4:7270459-7270478 None:intergenic 35.0%
AGAGAGAGAAAAAGCTATTG+CGG + chr5_4:7270638-7270657 Msa0834260:CDS 35.0%
GCCTGTATTCTAGTGATAAA+TGG - chr5_4:7270889-7270908 None:intergenic 35.0%
! TCACTAGAATACAGGCATTT+TGG + chr5_4:7270893-7270912 Msa0834260:three_prime_UTR 35.0%
! TGGTTTGCATCATATCATCA+TGG + chr5_4:7270944-7270963 Msa0834260:three_prime_UTR 35.0%
TATCATCATGGCAGATTTAC+CGG + chr5_4:7270956-7270975 Msa0834260:three_prime_UTR 35.0%
GAAAGAAACACTACCACAGA+AGG + chr5_4:7268040-7268059 Msa0834260:intron 40.0%
TTAACTACCGAGGAATTCCT+CGG + chr5_4:7268163-7268182 Msa0834260:intron 40.0%
!! CAGCCGGATTTTTAATTCCT+CGG + chr5_4:7268196-7268215 Msa0834260:intron 40.0%
CTGCCGAGGAATTAAAAATC+CGG - chr5_4:7268202-7268221 None:intergenic 40.0%
!! GATGAAGTTGTTGATGGTGA+TGG + chr5_4:7268316-7268335 Msa0834260:intron 40.0%
GATTAGAGGAAGAGACACAA+AGG + chr5_4:7268395-7268414 Msa0834260:intron 40.0%
GAGACACAAAGGAATTGAGA+AGG + chr5_4:7268406-7268425 Msa0834260:intron 40.0%
ACTACAAGTGCTTCCATCAA+AGG - chr5_4:7268476-7268495 None:intergenic 40.0%
CAGCTGTAGTTAACAACTGT+TGG + chr5_4:7268515-7268534 Msa0834260:intron 40.0%
GTGAGAGACAATAATAGGTC+CGG - chr5_4:7268707-7268726 None:intergenic 40.0%
AGTGCTTTGTAGGAATTCAG+AGG - chr5_4:7269382-7269401 None:intergenic 40.0%
! TGTCTGTGATAGTGCTTTGT+AGG - chr5_4:7269392-7269411 None:intergenic 40.0%
ATGCTGTTATAGGGAGACTT+TGG + chr5_4:7269626-7269645 Msa0834260:intron 40.0%
!! ACTTTGGAGTGATGAAGATG+AGG + chr5_4:7269642-7269661 Msa0834260:CDS 40.0%
GATGAAGATGAGGAAGCAAA+AGG + chr5_4:7269652-7269671 Msa0834260:CDS 40.0%
GTTGGCAGATAAAACTTAGC+TGG - chr5_4:7269767-7269786 None:intergenic 40.0%
GGTTTCTCGTTATCATCAGT+TGG - chr5_4:7269785-7269804 None:intergenic 40.0%
AGAAGTTCAAGTGTGGTTAC+AGG - chr5_4:7269908-7269927 None:intergenic 40.0%
CTTCTTGAGAAGTTCAAGTG+TGG - chr5_4:7269915-7269934 None:intergenic 40.0%
GAATGAAAGTGAGGAAGAAG+AGG + chr5_4:7269955-7269974 Msa0834260:CDS 40.0%
! ATCGCAAGCTTGAAGGTTTT+CGG + chr5_4:7270056-7270075 Msa0834260:CDS 40.0%
TTCTCTTGTAGCTTCTCATC+AGG - chr5_4:7270211-7270230 None:intergenic 40.0%
TGATGAGAAGCTACAAGAGA+AGG + chr5_4:7270210-7270229 Msa0834260:CDS 40.0%
GTTTGGTCACGTTAAACTTC+AGG + chr5_4:7270270-7270289 Msa0834260:CDS 40.0%
TCACGTTAAACTTCAGGATG+AGG + chr5_4:7270276-7270295 Msa0834260:CDS 40.0%
CAAAGCACAAGGAACTTGAA+AGG + chr5_4:7270326-7270345 Msa0834260:CDS 40.0%
ACTTGAAAGGGCTAAGAAGT+TGG + chr5_4:7270339-7270358 Msa0834260:CDS 40.0%
GAAGAAGAATGATCCTGAGA+AGG + chr5_4:7270369-7270388 Msa0834260:CDS 40.0%
CTATTGCTAAGAAGGAAGCA+TGG + chr5_4:7270398-7270417 Msa0834260:CDS 40.0%
AACTCTCTCTTCCCATTTCT+CGG - chr5_4:7270524-7270543 None:intergenic 40.0%
GGAAGAGAGAGTTCAATCAA+GGG + chr5_4:7270531-7270550 Msa0834260:CDS 40.0%
TCAATCAAGGGATCAGTTGA+AGG + chr5_4:7270543-7270562 Msa0834260:CDS 40.0%
AGGCAGAGAAGCAAAAGAAA+AGG + chr5_4:7270563-7270582 Msa0834260:CDS 40.0%
GGTCAGATAATATAGCTGAG+AGG + chr5_4:7270584-7270603 Msa0834260:CDS 40.0%
CCTCCGATAACAATGCAAAA+AGG - chr5_4:7270742-7270761 None:intergenic 40.0%
! CCTTTTTGCATTGTTATCGG+AGG + chr5_4:7270739-7270758 Msa0834260:three_prime_UTR 40.0%
CATCCATCAATTTCAACGTG+TGG + chr5_4:7270770-7270789 Msa0834260:three_prime_UTR 40.0%
AAGCCACACGTTGAAATTGA+TGG - chr5_4:7270776-7270795 None:intergenic 40.0%
AAGGTGAAGTGATGTGCTTG+TGG + chr5_4:7268059-7268078 Msa0834260:intron 45.0%
!! GATGGTGATGGTGAAGAAGA+AGG + chr5_4:7268328-7268347 Msa0834260:intron 45.0%
TCTGCGAGACACAGATAATG+AGG - chr5_4:7268751-7268770 None:intergenic 45.0%
! ACCATACCTTTGGGAAGAAG+CGG - chr5_4:7268847-7268866 None:intergenic 45.0%
GAAGCAAAAGGTTGTTGCAG+AGG + chr5_4:7269664-7269683 Msa0834260:CDS 45.0%
GTTGCAGAGGTTGTTGATGT+TGG + chr5_4:7269677-7269696 Msa0834260:CDS 45.0%
! CTGATGATAACGAGAAACCG+TGG + chr5_4:7269786-7269805 Msa0834260:CDS 45.0%
TAACGAGAAACCGTGGTTTC+AGG + chr5_4:7269793-7269812 Msa0834260:CDS 45.0%
AACGAGAAACCGTGGTTTCA+GGG + chr5_4:7269794-7269813 Msa0834260:CDS 45.0%
TTGTTGAGACCCTGAAACCA+CGG - chr5_4:7269806-7269825 None:intergenic 45.0%
AAGTGAGGAAGAAGAGGATG+TGG + chr5_4:7269961-7269980 Msa0834260:CDS 45.0%
! TAAGCCTTTTGTGTCTGAGC+TGG + chr5_4:7269985-7270004 Msa0834260:CDS 45.0%
AACTCGTAACTGTGCAGATC+CGG + chr5_4:7270081-7270100 Msa0834260:CDS 45.0%
TAACTGTGCAGATCCGGAAA+AGG + chr5_4:7270087-7270106 Msa0834260:CDS 45.0%
! CTCTTCTTAGCAGCCTTTTC+CGG - chr5_4:7270103-7270122 None:intergenic 45.0%
GGAAAAGGCTGCTAAGAAGA+GGG + chr5_4:7270102-7270121 Msa0834260:CDS 45.0%
GAAGATAGAGACACCAAGAG+AGG + chr5_4:7270124-7270143 Msa0834260:CDS 45.0%
TTGTAGCTTCTCATCAGGTG+CGG - chr5_4:7270206-7270225 None:intergenic 45.0%
GCTACAAGAGAAGGTGAAGA+AGG + chr5_4:7270219-7270238 Msa0834260:CDS 45.0%
GCTTCAAAGGAGCTTGTGTT+TGG + chr5_4:7270253-7270272 Msa0834260:CDS 45.0%
CTTCAGGATGAGGAAATGCT+AGG + chr5_4:7270286-7270305 Msa0834260:CDS 45.0%
TGAAAGGGCTAAGAAGTTGG+AGG + chr5_4:7270342-7270361 Msa0834260:CDS 45.0%
GGGTGAAGCTATTGCTAAGA+AGG + chr5_4:7270390-7270409 Msa0834260:CDS 45.0%
GGACAGAGCTTCAGGAATTA+AGG + chr5_4:7270432-7270451 Msa0834260:CDS 45.0%
GGGAAGAGAGAGTTCAATCA+AGG + chr5_4:7270530-7270549 Msa0834260:CDS 45.0%
AGAGAAAAAGCTATTGCGGC+CGG + chr5_4:7270642-7270661 Msa0834260:CDS 45.0%
GCACATCACTTCACCTTCTG+TGG - chr5_4:7268056-7268075 None:intergenic 50.0%
TTCTCGGCAGTTAACTACCG+AGG + chr5_4:7268153-7268172 Msa0834260:intron 50.0%
CTAACTGCCGAGGAATTCCT+CGG - chr5_4:7268173-7268192 None:intergenic 50.0%
! ATACCTTTGGGAAGAAGCGG+TGG - chr5_4:7268844-7268863 None:intergenic 50.0%
ACCGCTTCTTCCCAAAGGTA+TGG + chr5_4:7268843-7268862 Msa0834260:intron 50.0%
GTGTGGTTACAGGCTTCTCA+GGG - chr5_4:7269898-7269917 None:intergenic 50.0%
AGTGTGGTTACAGGCTTCTC+AGG - chr5_4:7269899-7269918 None:intergenic 50.0%
CCATCCAGCTCAGACACAAA+AGG - chr5_4:7269992-7270011 None:intergenic 50.0%
! CCTTTTGTGTCTGAGCTGGA+TGG + chr5_4:7269989-7270008 Msa0834260:CDS 50.0%
CTGAGCTGGATGGAGATGAT+CGG + chr5_4:7269999-7270018 Msa0834260:CDS 50.0%
CCTTTGGCGAAGCTCTTCAT+AGG - chr5_4:7270032-7270051 None:intergenic 50.0%
CCTATGAAGAGCTTCGCCAA+AGG + chr5_4:7270029-7270048 Msa0834260:CDS 50.0%
CAAGCTTGCGATGAAGCCTT+TGG - chr5_4:7270048-7270067 None:intergenic 50.0%
AGGCTTCATCGCAAGCTTGA+AGG + chr5_4:7270049-7270068 Msa0834260:CDS 50.0%
CGGAAAAGGCTGCTAAGAAG+AGG + chr5_4:7270101-7270120 Msa0834260:CDS 50.0%
AGGTGCGGGCTTACTTTCAT+TGG - chr5_4:7270191-7270210 None:intergenic 50.0%
TGTAGCTTCTCATCAGGTGC+GGG - chr5_4:7270205-7270224 None:intergenic 50.0%
GCAATAGCTTCACCCTTCTC+AGG - chr5_4:7270385-7270404 None:intergenic 50.0%
! GGAAGCATGGAAAGCAGCAA+TGG + chr5_4:7270411-7270430 Msa0834260:CDS 50.0%
GAGAAAAAGCTATTGCGGCC+GGG + chr5_4:7270643-7270662 Msa0834260:CDS 50.0%
TCCTCGGCAGTTAACTACCG+AGG + chr5_4:7268212-7268231 Msa0834260:intron 55.0%
CCTCGGCAGTTAACTACCGA+GGG + chr5_4:7268213-7268232 Msa0834260:intron 55.0%
CTCGGCAGTTAACTACCGAG+GGG + chr5_4:7268155-7268174 Msa0834260:intron 55.0%
TCGGCAGTTAACTACCGAGG+GGG + chr5_4:7268156-7268175 Msa0834260:intron 55.0%
TGGGAAGAAGCGGTGGATGA+CGG - chr5_4:7268837-7268856 None:intergenic 55.0%
CATCCACCGCTTCTTCCCAA+AGG + chr5_4:7268838-7268857 Msa0834260:intron 55.0%
!! TGCTGGTGTCGATGCTACAG+AGG + chr5_4:7269481-7269500 Msa0834260:intron 55.0%
GAAGGTGAAGAAGGATGCCG+CGG + chr5_4:7270228-7270247 Msa0834260:CDS 55.0%
!! AAGCTCCTTTGAAGCCTCCG+CGG - chr5_4:7270248-7270267 None:intergenic 55.0%
GCAGCAATGGACAGAGCTTC+AGG + chr5_4:7270424-7270443 Msa0834260:CDS 55.0%
TCCCTGTGACCTTCAAAGCC+CGG - chr5_4:7270664-7270683 None:intergenic 55.0%
!! GGCTTTGAAGGTCACAGGGA+AGG + chr5_4:7270664-7270683 Msa0834260:CDS 55.0%
CCTTCGGCACTTTACTGCCG+AGG - chr5_4:7268109-7268128 None:intergenic 60.0%
!! CCTCGGCAGTAAAGTGCCGA+AGG + chr5_4:7268106-7268125 Msa0834260:intron 60.0%
CCCTCGGTAGTTAACTGCCG+AGG - chr5_4:7268216-7268235 None:intergenic 60.0%
CGGCGAAAGGCGGAGTGATT+GGG - chr5_4:7268796-7268815 None:intergenic 60.0%
ACGGCGAAAGGCGGAGTGAT+TGG - chr5_4:7268797-7268816 None:intergenic 60.0%
! AAGAAGCGGTGGATGACGGC+GGG - chr5_4:7268833-7268852 None:intergenic 60.0%
! GAAGAAGCGGTGGATGACGG+CGG - chr5_4:7268834-7268853 None:intergenic 60.0%
! CTATTGCGGCCGGGCTTTGA+AGG + chr5_4:7270652-7270671 Msa0834260:CDS 60.0%
!! GCCGGGCTTTGAAGGTCACA+GGG + chr5_4:7270660-7270679 Msa0834260:CDS 60.0%
CCTCGGCAGTTAGCTGCAGC+CGG + chr5_4:7268180-7268199 Msa0834260:intron 65.0%
GGTGAAGAAGGATGCCGCGG+AGG + chr5_4:7270231-7270250 Msa0834260:CDS 65.0%
GGATGCCGCGGAGGCTTCAA+AGG + chr5_4:7270240-7270259 Msa0834260:CDS 65.0%
!! GGCCGGGCTTTGAAGGTCAC+AGG + chr5_4:7270659-7270678 Msa0834260:CDS 65.0%
CCGGCTGCAGCTAACTGCCG+AGG - chr5_4:7268183-7268202 None:intergenic 70.0%
GGTCGACGGACGGCGAAAGG+CGG - chr5_4:7268806-7268825 None:intergenic 70.0%
TCGGGTCGACGGACGGCGAA+AGG - chr5_4:7268809-7268828 None:intergenic 70.0%
! TGACGGCGGGTTCGGGTCGA+CGG - chr5_4:7268820-7268839 None:intergenic 70.0%
! CGGTGGATGACGGCGGGTTC+GGG - chr5_4:7268827-7268846 None:intergenic 70.0%
! GCGGTGGATGACGGCGGGTT+CGG - chr5_4:7268828-7268847 None:intergenic 70.0%
! GGCGGGTTCGGGTCGACGGA+CGG - chr5_4:7268816-7268835 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 7267996 7271010 7267996 ID=Msa0834260;Name=Msa0834260
chr5_4 mRNA 7267996 7271010 7267996 ID=Msa0834260-mRNA-1;Parent=Msa0834260;Name=Msa0834260-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0.5|0.5|1|1|2|299|360
chr5_4 exon 7267996 7268004 7267996 ID=Msa0834260-mRNA-1:exon:3169;Parent=Msa0834260-mRNA-1
chr5_4 exon 7269638 7271010 7269638 ID=Msa0834260-mRNA-1:exon:3170;Parent=Msa0834260-mRNA-1
chr5_4 CDS 7267996 7268004 7267996 ID=Msa0834260-mRNA-1:cds;Parent=Msa0834260-mRNA-1
chr5_4 CDS 7269638 7270711 7269638 ID=Msa0834260-mRNA-1:cds;Parent=Msa0834260-mRNA-1
chr5_4 three_prime_UTR 7270712 7271010 7270712 ID=Msa0834260-mRNA-1:three_prime_utr;Parent=Msa0834260-mRNA-1
Gene Sequence

>Msa0834260

ATGAAAATGGGAGACTTTGGAGTGATGAAGATGAGGAAGCAAAAGGTTGTTGCAGAGGTTGTTGATGTTGGTCAAGATTTGGAGTCTGTGATTCACGAGCATGCCAGTTTTTTCGACAAATTGGTTGAACTTATTCCAGCTAAGTTTTATCTGCCAACTGATGATAACGAGAAACCGTGGTTTCAGGGTCTCAACAAGAAACAGAAAGCAAAGGCAAAGAAAAAAACTATACAGAATATCAAGAAGTCTAAGGTGCAGCGTTTAGACCCTGAGAAGCCTGTAACCACACTTGAACTTCTCAAGAAGAGTTTGGGAAAAGAAAAAGCGAATGAAAGTGAGGAAGAAGAGGATGTGGTTAAGCCTTTTGTGTCTGAGCTGGATGGAGATGATCGGTCTGTGACCTATGAAGAGCTTCGCCAAAGGCTTCATCGCAAGCTTGAAGGTTTTCGGTCAACTCGTAACTGTGCAGATCCGGAAAAGGCTGCTAAGAAGAGGGAAGATAGAGACACCAAGAGAGGATATCATTATCAGGATAATAAACGCAAGAGGGATGATGAGACCAATGAAAGTAAGCCCGCACCTGATGAGAAGCTACAAGAGAAGGTGAAGAAGGATGCCGCGGAGGCTTCAAAGGAGCTTGTGTTTGGTCACGTTAAACTTCAGGATGAGGAAATGCTAGGTAAGAAGAGAAAAATTTCAAAGCACAAGGAACTTGAAAGGGCTAAGAAGTTGGAGGAACTGAAGAAGAATGATCCTGAGAAGGGTGAAGCTATTGCTAAGAAGGAAGCATGGAAAGCAGCAATGGACAGAGCTTCAGGAATTAAGGTCCATGACGATCCCAAATTGATAAAGAAAAGCATACATAAGGGGAAGAAGAAGCAAGAAAAAAATGCCGAGAAATGGGAAGAGAGAGTTCAATCAAGGGATCAGTTGAAGGCAGAGAAGCAAAAGAAAAGGTCAGATAATATAGCTGAGAGGATTAATGATAAGAAAATGAGGAAGATTGCAAAGAGAGAGAAAAAGCTATTGCGGCCGGGCTTTGAAGGTCACAGGGAAGGTTTTATTAATAGCAGTTCTGGCTAA
Protein sequence

>Msa0834260

MKMGDFGVMKMRKQKVVAEVVDVGQDLESVIHEHASFFDKLVELIPAKFYLPTDDNEKPWFQGLNKKQKAKAKKKTIQNIKKSKVQRLDPEKPVTTLELLKKSLGKEKANESEEEEDVVKPFVSELDGDDRSVTYEELRQRLHRKLEGFRSTRNCADPEKAAKKREDRDTKRGYHYQDNKRKRDDETNESKPAPDEKLQEKVKKDAAEASKELVFGHVKLQDEEMLGKKRKISKHKELERAKKLEELKKNDPEKGEAIAKKEAWKAAMDRASGIKVHDDPKLIKKSIHKGKKKQEKNAEKWEERVQSRDQLKAEKQKKRSDNIAERINDKKMRKIAKREKKLLRPGFEGHREGFINSSSG*