Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748430 | XP_039685298.1 | 79.698 | 729 | 50 | 10 | 26 | 663 | 1 | 722 | 0.0 | 1001 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748430 | sp|Q9Y222|DMTF1_HUMAN | 29.902 | 204 | 129 | 6 | 436 | 634 | 205 | 399 | 1.70e-14 | 80.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748430 | A0A396GV57 | 75.720 | 729 | 86 | 7 | 26 | 663 | 1 | 729 | 0.0 | 959 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006110 | Msa0748430 | 0.820380 | 7.072299e-53 | -8.615850e-47 |
| Msa0010770 | Msa0748430 | 0.807335 | 5.316409e-50 | -8.615850e-47 |
| Msa0020410 | Msa0748430 | 0.811022 | 8.616611e-51 | -8.615850e-47 |
| Msa0022550 | Msa0748430 | 0.827326 | 1.664041e-54 | -8.615850e-47 |
| Msa0026910 | Msa0748430 | 0.823257 | 1.527066e-53 | -8.615850e-47 |
| Msa0029630 | Msa0748430 | 0.807680 | 4.491492e-50 | -8.615850e-47 |
| Msa0030300 | Msa0748430 | 0.813627 | 2.326159e-51 | -8.615850e-47 |
| Msa0031180 | Msa0748430 | 0.817919 | 2.568596e-52 | -8.615850e-47 |
| Msa0031340 | Msa0748430 | 0.807163 | 5.779763e-50 | -8.615850e-47 |
| Msa0032470 | Msa0748430 | 0.842034 | 3.308366e-58 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748430 | MtrunA17_Chr7g0225681 | 75.720 | 729 | 86 | 7 | 26 | 663 | 1 | 729 | 0.0 | 959 |
| Msa0748430 | MtrunA17_Chr8g0373581 | 73.423 | 444 | 29 | 6 | 290 | 663 | 2 | 426 | 0.0 | 610 |
| Msa0748430 | MtrunA17_Chr8g0373591 | 81.413 | 269 | 29 | 5 | 26 | 273 | 1 | 269 | 9.80e-99 | 303 |
| Msa0748430 | MtrunA17_Chr8g0373591 | 62.963 | 135 | 40 | 5 | 181 | 309 | 45 | 175 | 5.71e-20 | 90.5 |
| Msa0748430 | MtrunA17_Chr1g0188151 | 96.739 | 92 | 3 | 0 | 572 | 663 | 1 | 92 | 4.17e-58 | 190 |
| Msa0748430 | MtrunA17_Chr1g0188141 | 84.444 | 90 | 13 | 1 | 26 | 114 | 1 | 90 | 3.15e-30 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748430 | AT5G41020.1 | 55.124 | 283 | 126 | 1 | 378 | 660 | 302 | 583 | 2.25e-112 | 350 |
Find 134 sgRNAs with CRISPR-Local
Find 327 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAACTGAAGACGACGA+GGG | 0.720222 | 5_2:-1666181 | Msa0748430:CDS |
| AAAAGCAATAAAAGTGAAGA+TGG | 0.528970 | 5_2:-1666097 | Msa0748430:CDS |
| AAAAGGACATTGGTCCCAAG+AGG | 0.666914 | 5_2:-1664604 | Msa0748430:CDS |
| AAAAGTGAAGATGGCGAGCA+AGG | 0.538269 | 5_2:-1666088 | Msa0748430:CDS |
| AAAATAAACCCATTCAATAT+AGG | 0.398922 | 5_2:+1666258 | None:intergenic |
| AAACCTAGATCTGCTCGAGA+GGG | 0.608007 | 5_2:-1664556 | Msa0748430:CDS |
| AAAGGGAGGGGAGGTGTAAG+TGG | 0.424706 | 5_2:-1664764 | Msa0748430:CDS |
| AAATGTCAAATTACACCCCG+AGG | 0.668903 | 5_2:-1664871 | Msa0748430:CDS |
| AAATGTGAAGCTGACAACCA+AGG | 0.682713 | 5_2:-1665797 | Msa0748430:intron |
| AAATTACACCCCGAGGTTAG+AGG | 0.442409 | 5_2:-1664864 | Msa0748430:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGTGATGTCCTATATTGAA+TGG | - | chr5_2:1664131-1664150 | Msa0748430:exon | 30.0% | |
| TGTGATGTCCTATATTGAAT+GGG | - | chr5_2:1664132-1664151 | Msa0748430:exon | 30.0% | |
| GAAAGAAGATGAAAAAGAAG+CGG | - | chr5_2:1664431-1664450 | Msa0748430:CDS | 30.0% | |
| GGGAAAGAAGAAAAAGAAAA+AGG | - | chr5_2:1664525-1664544 | Msa0748430:CDS | 30.0% | |
| GAAAAAGGAAAAATTGAGTG+AGG | - | chr5_2:1664540-1664559 | Msa0748430:CDS | 30.0% | |
| TCATCATCTTCACATTCATT+TGG | + | chr5_2:1664406-1664425 | None:intergenic | 30.0% | |
| AATGAATGTGAAGATGATGA+TGG | - | chr5_2:1664406-1664425 | Msa0748430:CDS | 30.0% | |
| GAAGAAGAAGAAACTAATTG+AGG | - | chr5_2:1665017-1665036 | Msa0748430:CDS | 30.0% | |
| AGTGACATTATAAGCAATGA+AGG | - | chr5_2:1665060-1665079 | Msa0748430:CDS | 30.0% | |
| TTGATCATTAACTTCACTGA+CGG | + | chr5_2:1665238-1665257 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 1664116 | 1666304 | 1664116 | ID=Msa0748430;Name=Msa0748430 |
| chr5_2 | mRNA | 1664116 | 1666304 | 1664116 | ID=Msa0748430-mRNA-1;Parent=Msa0748430;Name=Msa0748430-mRNA-1;_AED=0.01;_eAED=0.01;_QI=2|0.25|0.2|1|0|0|5|27|663 |
| chr5_2 | exon | 1664116 | 1665206 | 1664116 | ID=Msa0748430-mRNA-1:exon:481;Parent=Msa0748430-mRNA-1 |
| chr5_2 | exon | 1665339 | 1665547 | 1665339 | ID=Msa0748430-mRNA-1:exon:480;Parent=Msa0748430-mRNA-1 |
| chr5_2 | exon | 1665563 | 1665809 | 1665563 | ID=Msa0748430-mRNA-1:exon:479;Parent=Msa0748430-mRNA-1 |
| chr5_2 | exon | 1665825 | 1666049 | 1665825 | ID=Msa0748430-mRNA-1:exon:478;Parent=Msa0748430-mRNA-1 |
| chr5_2 | exon | 1666056 | 1666304 | 1666056 | ID=Msa0748430-mRNA-1:exon:477;Parent=Msa0748430-mRNA-1 |
| chr5_2 | five_prime_UTR | 1666303 | 1666304 | 1666303 | ID=Msa0748430-mRNA-1:five_prime_utr;Parent=Msa0748430-mRNA-1 |
| chr5_2 | CDS | 1666056 | 1666302 | 1666056 | ID=Msa0748430-mRNA-1:cds;Parent=Msa0748430-mRNA-1 |
| chr5_2 | CDS | 1665825 | 1666049 | 1665825 | ID=Msa0748430-mRNA-1:cds;Parent=Msa0748430-mRNA-1 |
| chr5_2 | CDS | 1665563 | 1665809 | 1665563 | ID=Msa0748430-mRNA-1:cds;Parent=Msa0748430-mRNA-1 |
| chr5_2 | CDS | 1665339 | 1665547 | 1665339 | ID=Msa0748430-mRNA-1:cds;Parent=Msa0748430-mRNA-1 |
| chr5_2 | CDS | 1664143 | 1665206 | 1664143 | ID=Msa0748430-mRNA-1:cds;Parent=Msa0748430-mRNA-1 |
| chr5_2 | three_prime_UTR | 1664116 | 1664142 | 1664116 | ID=Msa0748430-mRNA-1:three_prime_utr;Parent=Msa0748430-mRNA-1 |
| Gene Sequence |
>Msa0748430 ATGTATTATAAACATGTGATGTCCTATATTGAATGGGTTTATTTTGCAGTCATTGAAGAGGGGCACAGAACGAAGATGAAGACTCCGAGCAAGAAAAACAAAAGAACTGAAGACGACGAGGGTGAAACCAATGGAGGTGATCCATATATGTCCGAAAGCAATGTAGCTGATCTGCCTTTGACCAAAAGCAATAAAAGTGAAGATGGCGAGCAAGGCAAAAAGAAAAAGAAAAAGAAGAAGAAACTGAAAAGCAATCATAAGAAGTATAATGAGTTCAAAAGCAATGAATGTGAAGATGATGACCACGGAAAGAAGATGAAAAAGAAGCGGAAGCTAGTTGAAGGAAACACACTTGAGGAGGGTAACAACTTTAGAAGCAATGAAGGTGATAATGGCGAGCAGGGAAAGAAGAAAAAGAAAAAGGAAAAATTGAGTGAGGAAAGCAAACATAAGGAGTATAACGAGTTCAAAACTGACAACCAAGGAAAGAAGATGAAAAAGAAGCTAATTGAAGGAAGCACACTGAAGGAATGTAACGACTTTAGAAGCAATGATGATGAAGATGGTGAGCGAGAGAAAAAGATGAAGAAAAAGAAGAAACCAAGTGAAGAAGGCACACATAAGGAGTATAGTGAGTTCAAAAGCAATGATTGTGAAGATGACCAAGGAAAAAAGACGAAAAAGAAGCAGAAATTAATTGAAGGAAACACACTTAATGAAAGCAATGGAGGTGAAGAGGGTGATCAAGACAAGAAGATGAAGAAAAAGAAGAAGAAACTAAGTGAAGAAAGTAAACATAAGGAGTATAGCAAGTTTGAACCAAATGAATGTGAAGATGATGATGGCCAAGGAAAGAAAATGAAGAAGAAGAAGAAACTAATTGAGGAAGCCAAACTTAAGGAGTATAGTGACATTATAAGCAATGAAGGTGATGCTGATGCTTGTTTCTCCGTCAGTGAAGTTAATGATCAAGGCAAAAAGATGAAGAAAAAAAAGAAAGCAAAGACAGTAACAAGCGAGTCTCCTAACTGTGCACATAATGAAACTCCTAACTCTGCACATAATGGAACATCTAAACGCAAACAAGTAACTTGGGCTGATGAGGTGGAGGAGAAGTTATGTTGTGACGGCTTATTGCGTGGGAAAAGGTATACACCCGAAGAAGATGAGAAGATCAAAGCAGCTGTTTTGGAGTATATAGATTCTCATGGTTTGGGAGATGAAGGTCTTGATATGGTTCTAAATGTCAAATTACACCCCGAGGTTAGAGGCTGTTGGAAAGAAATCGCAAAAAGCATACCTGAGAGGCCTCATTACAGTGTGTATAACCGTGCTCATGCTTTGTTAGAAAGGGAGGGGAGGTGTAAGTGGACACCTGAAGAGCTGGAATTTATTGAAAAGACCTACGAGCAACATGGATCTAGTTGGAGGGCAGTAGCCGACGCTTTGGGTAAAAGTCGAGATCAGGTAAAAGATGCGTGGCGCCGATTAAAATATACCAAAACAAAAAAAGGACATTGGTCCCAAGAGGAGTATCAAAATTTATTTAACTTAGTAAACCTAGATCTGCTCGAGAGGGCTAAAGAGCCTTATAAAAAATCACAACATGGTATGTTGCGAGATAATATTTGTTGGGAGGCAATTGGTCACAAATTGGAGACTCGAAACAGTGCATTTTGCTGCAAGAAGTGGTACGAGCAATTAACATCAGCATTGGTTGCTAGTGGTGATTGGTGTGATACCGATGACTTCCGCTTGATAAACGCTTTATATGCTTTGGATGCTTGCTGCATGGAGGAGGTAGACTGGGACAATCTACTTGAGCATAGGTCTGGTGATGTATGCAGGAAACGATGGGAACAAATGATCCACCATATTGGGGAGCATGCAGCCAAGTCATTCATTGAGCAGGTGGAAGTTCTGGCAAAGAGATTCTGCCCTAACCTGTTGAAAGATAGAGAGGCCTTTGACAACAAACCTGTGATTTGTTGA |
| Protein sequence |
>Msa0748430 MYYKHVMSYIEWVYFAVIEEGHRTKMKTPSKKNKRTEDDEGETNGGDPYMSESNVADLPLTKSNKSEDGEQGKKKKKKKKKLKSNHKKYNEFKSNECEDDDHGKKMKKKRKLVEGNTLEEGNNFRSNEGDNGEQGKKKKKKEKLSEESKHKEYNEFKTDNQGKKMKKKLIEGSTLKECNDFRSNDDEDGEREKKMKKKKKPSEEGTHKEYSEFKSNDCEDDQGKKTKKKQKLIEGNTLNESNGGEEGDQDKKMKKKKKKLSEESKHKEYSKFEPNECEDDDGQGKKMKKKKKLIEEAKLKEYSDIISNEGDADACFSVSEVNDQGKKMKKKKKAKTVTSESPNCAHNETPNSAHNGTSKRKQVTWADEVEEKLCCDGLLRGKRYTPEEDEKIKAAVLEYIDSHGLGDEGLDMVLNVKLHPEVRGCWKEIAKSIPERPHYSVYNRAHALLEREGRCKWTPEELEFIEKTYEQHGSSWRAVADALGKSRDQVKDAWRRLKYTKTKKGHWSQEEYQNLFNLVNLDLLERAKEPYKKSQHGMLRDNICWEAIGHKLETRNSAFCCKKWYEQLTSALVASGDWCDTDDFRLINALYALDACCMEEVDWDNLLEHRSGDVCRKRWEQMIHHIGEHAAKSFIEQVEVLAKRFCPNLLKDREAFDNKPVIC* |
