AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1272600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1272600 MtrunA17_Chr8g0337821 96.078 867 31 3 1 865 1 866 0.0 1718
Msa1272600 MtrunA17_Chr7g0243471 77.778 495 100 4 378 865 84 575 0.0 763
Msa1272600 MtrunA17_Chr7g0243471 58.537 123 37 3 187 308 15 124 5.13e-31 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1272600 AT1G17070.1 53.968 882 343 15 3 860 1 843 0.0 838
Msa1272600 AT2G42330.2 48.690 840 330 15 34 865 6 752 0.0 727
Msa1272600 AT2G42330.1 48.690 840 330 15 34 865 6 752 0.0 727
Msa1272600 AT2G42330.3 48.690 840 330 15 34 865 6 752 0.0 727

Find 216 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACCATGTCTCCTCATTT+TGG 0.120770 8_3:-6367022 None:intergenic
TGAAGTTACAAAATGGTATT+TGG 0.125933 8_3:+6366602 Msa1272600:CDS
CCTGGTTTAGGGTTAGGTTT+TGG 0.151973 8_3:+6364833 Msa1272600:CDS
CACAACGGTCATCAAATATA+TGG 0.248012 8_3:+6366948 Msa1272600:CDS
TCCATATATAACATCGTCTT+TGG 0.271281 8_3:-6364627 None:intergenic
TCATTTGCCAAAAGTTCTTT+TGG 0.273610 8_3:-6366642 None:intergenic
TTCAAATTGAGTACTGTTCT+TGG 0.275822 8_3:+6366285 Msa1272600:CDS
CTCATCAACATTATCCTTAT+CGG 0.278789 8_3:-6364774 None:intergenic
CCCACTGCACTTGATAGTTT+TGG 0.287889 8_3:-6366159 None:intergenic
TCTCTCCTTCTCCCTCTTCA+TGG 0.290858 8_3:-6365002 None:intergenic
TTCCCTTGCTCAATGCTTTA+CGG 0.291345 8_3:+6365771 Msa1272600:CDS
GAAGAAGATAGGCCTGGTTT+AGG 0.292810 8_3:+6364821 Msa1272600:CDS
AAGAAGCCGGGCATTGGTTT+AGG 0.293073 8_3:+6365049 Msa1272600:CDS
TCTGAAACCAATTGAGTATA+AGG 0.301102 8_3:-6365994 None:intergenic
ACTTATTCCAAAAGAACTTT+TGG 0.305783 8_3:+6366635 Msa1272600:CDS
GAAGGTGGCCAATGGGTTAA+TGG 0.309537 8_3:+6364560 Msa1272600:CDS
AGTATCTCGGGGTCTGGTTT+AGG 0.309623 8_3:+6364863 Msa1272600:CDS
TTGTGCGTTCTACCAGATTT+AGG 0.311130 8_3:-6366930 None:intergenic
ACCAAAGACGATGTTATATA+TGG 0.321878 8_3:+6364626 Msa1272600:CDS
GAGGCTTATGCTCGGGATAA+TGG 0.323040 8_3:+6366894 Msa1272600:CDS
TTTGTAACTTCATCAAAGTT+AGG 0.326962 8_3:-6366591 None:intergenic
TTGCCAGCTGTCAGAGTATC+TGG 0.339471 8_3:+6366024 Msa1272600:CDS
TTCTTCTCTGTCTGCAAAAC+GGG 0.347374 8_3:-6365271 None:intergenic
CTTGAGAAGATGGGTTATAA+AGG 0.348414 8_3:+6365136 Msa1272600:CDS
AGGAGAAGATAAGCTATATC+AGG 0.352380 8_3:+6366748 Msa1272600:CDS
TTTGGGTCGGCTAGTATCTC+GGG 0.355857 8_3:+6364851 Msa1272600:CDS
TGATCAAGATTCTGACTTGC+CGG 0.356856 8_3:-6366453 None:intergenic
ACTTTGATGAAGTTACAAAA+TGG 0.363094 8_3:+6366595 Msa1272600:CDS
TTACTGTTCAAACCTAAATC+TGG 0.369481 8_3:+6366918 Msa1272600:CDS
GTCATCACCTTATACTCAAT+TGG 0.369878 8_3:+6365987 Msa1272600:CDS
TATCAAGTGCAGTGGGTACT+TGG 0.373265 8_3:+6366166 Msa1272600:CDS
TCGAAATCCATCTCATGGTT+TGG 0.376763 8_3:+6365903 Msa1272600:CDS
AATCTGATGAGAATGATAAT+CGG 0.379308 8_3:+6364930 Msa1272600:CDS
CAAGCTGCTGCAAGCATGGC+TGG 0.379886 8_3:+6366828 Msa1272600:CDS
TGCAAGACAGCCTTCAGCTT+TGG 0.383113 8_3:-6366411 None:intergenic
TTCAGAAGATTTATGATATG+AGG 0.386976 8_3:+6365437 Msa1272600:CDS
GATAGGCCTGGTTTAGGGTT+AGG 0.390604 8_3:+6364827 Msa1272600:CDS
GAGAGTCATACAAAGGGAAT+TGG 0.392137 8_3:+6365103 Msa1272600:CDS
AAGAAGATAGGCCTGGTTTA+GGG 0.393008 8_3:+6364822 Msa1272600:CDS
TTGGGTTATGAACTGGGCTT+CGG 0.393328 8_3:+6366482 Msa1272600:CDS
CATCCATGGCTACCCCTGCT+AGG 0.404541 8_3:+6366228 Msa1272600:CDS
GCCCTCAACTTTGCCTCAAT+AGG 0.405496 8_3:-6365196 None:intergenic
GTGTCAACCATCAGATGAAT+TGG 0.408091 8_3:-6366510 None:intergenic
AGGAGGGAGCTGTGGATGTT+GGG 0.409566 8_3:+6365074 Msa1272600:CDS
GATCAAGAGATGGAGAATTT+CGG 0.416250 8_3:+6364521 Msa1272600:CDS
GAAGAGGGAGAAGGAGAGAT+TGG 0.416692 8_3:+6365006 Msa1272600:CDS
CTTCTTTGCCAAGTGGCTTA+CGG 0.416957 8_3:+6366542 Msa1272600:CDS
GAAGAAGAAGGGGAAGAAGC+CGG 0.421415 8_3:+6365036 Msa1272600:CDS
TCAAATCTCTATCAATCTCT+TGG 0.426322 8_3:-6365591 None:intergenic
CCTCCAGCTTGTGCCCTAGC+AGG 0.432848 8_3:-6366242 None:intergenic
TTCGAAGAGGATCCCATCCT+TGG 0.432902 8_3:-6365885 None:intergenic
GGTCATCAAATATATGGGTT+TGG 0.434302 8_3:+6366954 Msa1272600:CDS
CCCATCATCATCAACGTCTT+CGG 0.439994 8_3:-6364663 None:intergenic
TTGAGAAGATGGGTTATAAA+GGG 0.445982 8_3:+6365137 Msa1272600:CDS
TGCGTTTGGGAAGATGATTA+AGG 0.446702 8_3:+6364973 Msa1272600:CDS
TCAATTTGAAACGAATTGTC+TGG 0.447524 8_3:-6366272 None:intergenic
TCATCAACATTATCCTTATC+GGG 0.448438 8_3:-6364773 None:intergenic
GGCTATTCCAATTCATCTGA+TGG 0.452495 8_3:+6366503 Msa1272600:CDS
CAGGAGGGAGCTGTGGATGT+TGG 0.458207 8_3:+6365073 Msa1272600:CDS
CTTCTTCTCTGTCTGCAAAA+CGG 0.458538 8_3:-6365272 None:intergenic
CAGTGGAAGGCATTGCTTCA+AGG 0.460014 8_3:+6365937 Msa1272600:CDS
TTACAAAATGGTATTTGGGC+TGG 0.461685 8_3:+6366607 Msa1272600:CDS
AGGATGATGGTGAAGAAGAT+AGG 0.462708 8_3:+6364810 Msa1272600:CDS
GCCGGGCATTGGTTTAGGTC+AGG 0.463580 8_3:+6365054 Msa1272600:CDS
ATCTGATGAGAATGATAATC+GGG 0.464767 8_3:+6364931 Msa1272600:CDS
GACAGAGAAGAAGACTGAAC+CGG 0.467128 8_3:+6365282 Msa1272600:CDS
CCAGTTGAAACAAAATTCAC+AGG 0.467449 8_3:-6364740 None:intergenic
TGATGAGAATTTGAAAGAAC+AGG 0.467505 8_3:+6364790 Msa1272600:CDS
TCTATCAATCTCTTGGATGT+TGG 0.469174 8_3:-6365584 None:intergenic
TGTAACTGCTAAGGAGTTGT+TGG 0.469240 8_3:+6365384 Msa1272600:CDS
GAAGTTACAAAATGGTATTT+GGG 0.470045 8_3:+6366603 Msa1272600:CDS
TTGGAGGAGAAGAAGAAGAA+GGG 0.470912 8_3:+6365025 Msa1272600:CDS
AATCGACTCTCTAAATCAAA+AGG 0.472047 8_3:+6366992 Msa1272600:CDS
TCGGCTAGTATCTCGGGGTC+TGG 0.472352 8_3:+6364857 Msa1272600:CDS
TCTGACTTGCCGGGTTCACT+TGG 0.475554 8_3:-6366443 None:intergenic
AATGAAGCTGCTTGAGAAGA+TGG 0.478922 8_3:+6365126 Msa1272600:CDS
GTTGGAGAAGAGAACACCTT+AGG 0.479578 8_3:+6365733 Msa1272600:CDS
AAGAAGAAGGGGAAGAAGCC+GGG 0.485235 8_3:+6365037 Msa1272600:CDS
CATATGCTATATTGTCCCCT+TGG 0.485482 8_3:+6366334 Msa1272600:CDS
AGCATGGCTGGTGGCGCAGA+TGG 0.487215 8_3:+6366840 Msa1272600:CDS
ATCCATGGCTACCCCTGCTA+GGG 0.489307 8_3:+6366229 Msa1272600:CDS
AAGGGGAAGAAGCCGGGCAT+TGG 0.491690 8_3:+6365043 Msa1272600:CDS
TATACTGAAGCTCAGGCATT+GGG 0.492735 8_3:-6365531 None:intergenic
TGCCCTAGCAGGGGTAGCCA+TGG 0.494447 8_3:-6366231 None:intergenic
CTTATCTTCTCCTTCAAACC+AGG 0.494860 8_3:-6366738 None:intergenic
ACCCCTGCTAGGGCACAAGC+TGG 0.496535 8_3:+6366239 Msa1272600:CDS
CTTGGATTTAACGAATCAAA+GGG 0.496606 8_3:+6365232 Msa1272600:CDS
CTGTATTTGATTCTGATAGC+TGG 0.496901 8_3:+6366361 Msa1272600:CDS
GATCAAGATTCTGACTTGCC+GGG 0.497039 8_3:-6366452 None:intergenic
TTATACTGAAGCTCAGGCAT+TGG 0.497443 8_3:-6365532 None:intergenic
ATCCAATGTTAATGTTCCTA+AGG 0.499131 8_3:-6365749 None:intergenic
TCAGCTTTGGTACGATAAAA+CGG 0.499436 8_3:-6366398 None:intergenic
AAGAGATTGATAGAGATTTG+AGG 0.504347 8_3:+6365593 Msa1272600:CDS
CACCTTAGGAACATTAACAT+TGG 0.505825 8_3:+6365747 Msa1272600:CDS
TCCTATTGAGGCAAAGTTGA+GGG 0.506308 8_3:+6365195 Msa1272600:CDS
ATAACGTAGCACTAATAGTT+CGG 0.507572 8_3:+6365551 Msa1272600:CDS
TCCGAAGACGTTGATGATGA+TGG 0.510831 8_3:+6364662 Msa1272600:CDS
ATCTTATTGAGGCTTATGCT+CGG 0.511086 8_3:+6366886 Msa1272600:CDS
TCTTGGATTTAACGAATCAA+AGG 0.511423 8_3:+6365231 Msa1272600:CDS
ATAGAGATTTGAGGAAAGAG+AGG 0.513041 8_3:+6365602 Msa1272600:CDS
GCCAAAACTATCAAGTGCAG+TGG 0.513710 8_3:+6366158 Msa1272600:CDS
ATTGGAGGAGAAGAAGAAGA+AGG 0.514247 8_3:+6365024 Msa1272600:CDS
GTATCAATACTTGGCATGCA+CGG 0.515579 8_3:+6366046 Msa1272600:CDS
ACAACGGTCATCAAATATAT+GGG 0.518474 8_3:+6366949 Msa1272600:CDS
ACAACAAGCTGCTGCAAGCA+TGG 0.518724 8_3:+6366824 Msa1272600:CDS
TCCACACATGGGTGCATCCA+TGG 0.522047 8_3:+6366214 Msa1272600:CDS
TGGAGGAGAAGAAGAAGAAG+GGG 0.524605 8_3:+6365026 Msa1272600:CDS
TCAGAAGATTTATGATATGA+GGG 0.525953 8_3:+6365438 Msa1272600:CDS
TCTTATTGAGGCTTATGCTC+GGG 0.526021 8_3:+6366887 Msa1272600:CDS
TCCATCTCATGGTTTGGAGT+TGG 0.526127 8_3:+6365909 Msa1272600:CDS
GGTTGTGCAACCTGGTTTGA+AGG 0.528723 8_3:+6366728 Msa1272600:CDS
GCTACGTTATACTGAAGCTC+AGG 0.531815 8_3:-6365538 None:intergenic
GAACCAGGCTGTTGAAGGTA+TGG 0.534382 8_3:+6366704 Msa1272600:CDS
ATAGTAGCAAGAACTGAAGA+AGG 0.534632 8_3:-6366117 None:intergenic
TTTAGGTCAGGAGGGAGCTG+TGG 0.534834 8_3:+6365066 Msa1272600:CDS
AATGCTGGTCGTGGTGTGAA+TGG 0.539364 8_3:+6364890 Msa1272600:CDS
GAGGGAGAAGGAGAGATTGG+AGG 0.540333 8_3:+6365009 Msa1272600:CDS
TATCAGAATGTTCCAAGGAT+GGG 0.541188 8_3:+6365873 Msa1272600:CDS
CAGAAGATTTATGATATGAG+GGG 0.544424 8_3:+6365439 Msa1272600:CDS
GGAAAAGTATGTAACTGCTA+AGG 0.545075 8_3:+6365375 Msa1272600:CDS
TTATCAGAATGTTCCAAGGA+TGG 0.545083 8_3:+6365872 Msa1272600:CDS
CCGAAGACGTTGATGATGAT+GGG 0.545388 8_3:+6364663 Msa1272600:CDS
TATCAATACTTGGCATGCAC+GGG 0.546744 8_3:+6366047 Msa1272600:CDS
TCTGGTTTAGGATTTAATGC+TGG 0.547046 8_3:+6364875 Msa1272600:CDS
CCTCTTCGAAATCCATCTCA+TGG 0.547824 8_3:+6365898 Msa1272600:CDS
CTCCAGCTTGTGCCCTAGCA+GGG 0.548394 8_3:-6366241 None:intergenic
TGAAAAGATAATGAGTGTGC+TGG 0.550040 8_3:+6365705 Msa1272600:CDS
GGTATGGAGGTTGTGCAACC+TGG 0.550151 8_3:+6366720 Msa1272600:CDS
TCATCTGATGGTTGACACAA+TGG 0.550438 8_3:+6366515 Msa1272600:CDS
TGGTCTTGGGAAGAATGAGC+AGG 0.554574 8_3:+6365162 Msa1272600:CDS
TTATACTCAATTGGTTTCAG+AGG 0.556724 8_3:+6365996 Msa1272600:CDS
ATCAAGTGCAGTGGGTACTT+GGG 0.558150 8_3:+6366167 Msa1272600:CDS
ACAGAGAAGAAGACTGAACC+GGG 0.558425 8_3:+6365283 Msa1272600:CDS
AGAATTCACCATTAACCCAT+TGG 0.558800 8_3:-6364568 None:intergenic
AGCAGCTTGTTGTTGAGCAG+AGG 0.559345 8_3:-6366814 None:intergenic
CAAAACGGGCAACGGCATAG+TGG 0.559472 8_3:-6365257 None:intergenic
GAGTATCTGGTATCAATACT+TGG 0.559939 8_3:+6366037 Msa1272600:CDS
TATAGATGAGGATCAAGAGA+TGG 0.560053 8_3:+6364511 Msa1272600:CDS
GCTGCTGCAAGCATGGCTGG+TGG 0.561489 8_3:+6366831 Msa1272600:CDS
ACTGTAGGCTGCACAACCCC+CGG 0.561582 8_3:-6365301 None:intergenic
GCATATGCAGAACCATCACT+TGG 0.561871 8_3:-6366318 None:intergenic
GAGAGCCATGAAGAGGGAGA+AGG 0.561897 8_3:+6364997 Msa1272600:CDS
GAATCAAATACAGTCTTCCA+AGG 0.562070 8_3:-6366351 None:intergenic
GGAGTTGGTATCACAGTGGA+AGG 0.562462 8_3:+6365924 Msa1272600:CDS
AAAGGAGAAGTTGGAAGCTG+AGG 0.562740 8_3:+6365651 Msa1272600:CDS
TTTGAGTTCGAGCTTTCTCT+CGG 0.562831 8_3:-6364604 None:intergenic
TTAGGATTTAATGCTGGTCG+TGG 0.567508 8_3:+6364881 Msa1272600:CDS
AGATGACTTCCAAGTGAACC+CGG 0.567771 8_3:+6366434 Msa1272600:CDS
GGTGCATGGCACCCAAGTGA+TGG 0.568932 8_3:+6366306 Msa1272600:CDS
ATGATGAACCAGGCTGTTGA+AGG 0.577293 8_3:+6366699 Msa1272600:CDS
GGGGTTGTGCAGCCTACAGT+TGG 0.578126 8_3:+6365304 Msa1272600:CDS
TAAGCAAGAAGAAGAGTCAG+AGG 0.579514 8_3:+6365411 Msa1272600:CDS
CCTCCATACCTTCAACAGCC+TGG 0.581127 8_3:-6366707 None:intergenic
GAGCGTGAAAGATCTTATTG+AGG 0.582014 8_3:+6366875 Msa1272600:CDS
AAAGGTCTATGCCCAAAATG+AGG 0.582372 8_3:+6367010 Msa1272600:CDS
TAGAGATTTGAGGAAAGAGA+GGG 0.583409 8_3:+6365603 Msa1272600:CDS
ACCAACTCCAAACCATGAGA+TGG 0.585318 8_3:-6365910 None:intergenic
TGAGAAGATGGGTTATAAAG+GGG 0.586441 8_3:+6365138 Msa1272600:CDS
TCTGTCTGCAAAACGGGCAA+CGG 0.587541 8_3:-6365265 None:intergenic
AAATTCGAGAGTCATACAAA+GGG 0.591053 8_3:+6365097 Msa1272600:CDS
ATACAAAACCGTAAGCCACT+TGG 0.593433 8_3:-6366550 None:intergenic
TTGTTTATCAGAATGTTCCA+AGG 0.593879 8_3:+6365868 Msa1272600:CDS
ATCCTATTGAGGCAAAGTTG+AGG 0.595316 8_3:+6365194 Msa1272600:CDS
TAAGGAGAGAGCCATGAAGA+GGG 0.595678 8_3:+6364991 Msa1272600:CDS
ATACCAGATACTCTGACAGC+TGG 0.595723 8_3:-6366027 None:intergenic
CAGTTGGAAGAACACGTTCG+TGG 0.598877 8_3:+6365320 Msa1272600:CDS
GAACGTGTTCTTCCAACTGT+AGG 0.599260 8_3:-6365316 None:intergenic
GAAATTCGAGAGTCATACAA+AGG 0.599821 8_3:+6365096 Msa1272600:CDS
GATGGTGAAGAAGATAGGCC+TGG 0.601036 8_3:+6364815 Msa1272600:CDS
GGCATTGGTTTAGGTCAGGA+GGG 0.601669 8_3:+6365058 Msa1272600:CDS
GGAGAAGATAAGCTATATCA+GGG 0.605602 8_3:+6366749 Msa1272600:CDS
AATCAAATACAGTCTTCCAA+GGG 0.606618 8_3:-6366350 None:intergenic
ATTCGATACAAGCTCAATTG+TGG 0.607064 8_3:+6366669 Msa1272600:CDS
TTAGAAGATGGATATAGATG+AGG 0.607275 8_3:+6364499 None:intergenic
TGGAGATCTTCTTTGCCAAG+TGG 0.608455 8_3:+6366535 Msa1272600:CDS
GGATGCACCCATGTGTGGAT+AGG 0.608809 8_3:-6366210 None:intergenic
TCCTGACCTAAACCAATGCC+CGG 0.610190 8_3:-6365055 None:intergenic
ATGAAGCTGCTTGAGAAGAT+GGG 0.610235 8_3:+6365127 Msa1272600:CDS
TCCAGCTTGTGCCCTAGCAG+GGG 0.617024 8_3:-6366240 None:intergenic
TGGAACATTCTGATAAACAA+AGG 0.617370 8_3:-6365865 None:intergenic
AGACTATTCCTATCCACACA+TGG 0.617573 8_3:+6366202 Msa1272600:CDS
GCCATGGATGCACCCATGTG+TGG 0.620709 8_3:-6366215 None:intergenic
TTTGTCTGATTTGAATGCTG+AGG 0.621521 8_3:+6365486 Msa1272600:CDS
GGGCATTGGTTTAGGTCAGG+AGG 0.622016 8_3:+6365057 Msa1272600:CDS
ATGCCCAAAATGAGGAGACA+TGG 0.624654 8_3:+6367018 Msa1272600:CDS
TGTCCGTAAAGCATTGAGCA+AGG 0.624936 8_3:-6365774 None:intergenic
CCAAAACCTAACCCTAAACC+AGG 0.625240 8_3:-6364833 None:intergenic
ATATCAGGGTAAGTGAGCAA+AGG 0.626469 8_3:+6366763 Msa1272600:CDS
GAGAAGATGGGTTATAAAGG+GGG 0.630333 8_3:+6365139 Msa1272600:CDS
GTCCGTAAAGCATTGAGCAA+GGG 0.632161 8_3:-6365773 None:intergenic
TGAGTACTGTTCTTGGTGCA+TGG 0.632481 8_3:+6366292 Msa1272600:CDS
GAATGCTGAGGAGAAAGCAA+AGG 0.632682 8_3:+6365498 Msa1272600:CDS
CATGCACAAAAGATTTGCTG+AGG 0.634301 8_3:+6365795 Msa1272600:CDS
AATTTGAAAGAACAGGATGA+TGG 0.635084 8_3:+6364797 Msa1272600:CDS
GGGATCCTGAACCAATGCTA+CGG 0.635248 8_3:+6366067 Msa1272600:CDS
CCATGAGATGGATTTCGAAG+AGG 0.636233 8_3:-6365898 None:intergenic
ATGAGTGTGCTGGACAGAGT+TGG 0.636674 8_3:+6365715 Msa1272600:CDS
TTAAGGAGAGAGCCATGAAG+AGG 0.637194 8_3:+6364990 Msa1272600:CDS
AGAAGATTTATGATATGAGG+GGG 0.637702 8_3:+6365440 Msa1272600:CDS
CCAAAACTATCAAGTGCAGT+GGG 0.645728 8_3:+6366159 Msa1272600:CDS
TTGGGTCGGCTAGTATCTCG+GGG 0.651866 8_3:+6364852 Msa1272600:CDS
TCTGTTAAAACTCGTACCTG+CGG 0.658982 8_3:-6365463 None:intergenic
ATCATCTTCCCAAACGCAGT+AGG 0.663558 8_3:-6364968 None:intergenic
GACTATTCCTATCCACACAT+GGG 0.664132 8_3:+6366203 Msa1272600:CDS
CCAGGCTGTTGAAGGTATGG+AGG 0.671559 8_3:+6366707 Msa1272600:CDS
AGAGATGGAGAATTTCGGAA+CGG 0.680307 8_3:+6364526 Msa1272600:CDS
ATAGGAATAGTCTCAAGATG+TGG 0.683050 8_3:-6366192 None:intergenic
GGTCTTGGGAAGAATGAGCA+GGG 0.684826 8_3:+6365163 Msa1272600:CDS
CATATGCAGAACCATCACTT+GGG 0.687563 8_3:-6366317 None:intergenic
ATCAAATACAGTCTTCCAAG+GGG 0.688889 8_3:-6366349 None:intergenic
CCTGCTAGGGCACAAGCTGG+AGG 0.690416 8_3:+6366242 Msa1272600:CDS
TGGTCTTGATATGATGAACC+AGG 0.696080 8_3:+6366689 Msa1272600:CDS
CAGAGAAGAAGACTGAACCG+GGG 0.707743 8_3:+6365284 Msa1272600:CDS
AGAGAAGAAGACTGAACCGG+GGG 0.713315 8_3:+6365285 Msa1272600:CDS
GTTTGGAGTTGGTATCACAG+TGG 0.727402 8_3:+6365920 Msa1272600:CDS
AAATCTGGTAGAACGCACAA+CGG 0.732054 8_3:+6366933 Msa1272600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTCTTTATTTTTTTTCTT+CGG - chr8_3:6365355-6365374 None:intergenic 15.0%
!! GAAGAAAAAAAATAAAGAAG+AGG + chr8_3:6365354-6365373 Msa1272600:CDS 20.0%
!!! AGATGATTGTTTTGATATAT+GGG + chr8_3:6365960-6365979 Msa1272600:CDS 20.0%
!!! AGAATCTTGATCAATTTTAT+TGG + chr8_3:6366463-6366482 Msa1272600:CDS 20.0%
!!! GAATCTTGATCAATTTTATT+GGG + chr8_3:6366464-6366483 Msa1272600:CDS 20.0%
!! GATGTTATATATGGAGTTTT+TGG + chr8_3:6364635-6364654 Msa1272600:CDS 25.0%
!! AAAAAGCGAAGAAAAGATTT+TGG + chr8_3:6364695-6364714 Msa1272600:CDS 25.0%
! AATCTGATGAGAATGATAAT+CGG + chr8_3:6364930-6364949 Msa1272600:CDS 25.0%
!!! TCTTTATTTTTTTTCTTCGG+CGG - chr8_3:6365352-6365371 None:intergenic 25.0%
! TTCAGAAGATTTATGATATG+AGG + chr8_3:6365437-6365456 Msa1272600:CDS 25.0%
! TCAGAAGATTTATGATATGA+GGG + chr8_3:6365438-6365457 Msa1272600:CDS 25.0%
!! ACTTAGCTTGAAAAAAGAAA+AGG + chr8_3:6365633-6365652 Msa1272600:CDS 25.0%
!!! GAGATGATTGTTTTGATATA+TGG + chr8_3:6365959-6365978 Msa1272600:CDS 25.0%
!! AATTTTATTGGGTTATGAAC+TGG + chr8_3:6366475-6366494 Msa1272600:CDS 25.0%
!! ATTTTATTGGGTTATGAACT+GGG + chr8_3:6366476-6366495 Msa1272600:CDS 25.0%
! TTTGTAACTTCATCAAAGTT+AGG - chr8_3:6366594-6366613 None:intergenic 25.0%
! ACTTTGATGAAGTTACAAAA+TGG + chr8_3:6366595-6366614 Msa1272600:CDS 25.0%
! TGAAGTTACAAAATGGTATT+TGG + chr8_3:6366602-6366621 Msa1272600:CDS 25.0%
! GAAGTTACAAAATGGTATTT+GGG + chr8_3:6366603-6366622 Msa1272600:CDS 25.0%
!! ACTTATTCCAAAAGAACTTT+TGG + chr8_3:6366635-6366654 Msa1272600:CDS 25.0%
ACCAAAGACGATGTTATATA+TGG + chr8_3:6364626-6364645 Msa1272600:CDS 30.0%
TCCATATATAACATCGTCTT+TGG - chr8_3:6364630-6364649 None:intergenic 30.0%
!! AAAAGATTTTGGAAGGAAAC+AGG + chr8_3:6364706-6364725 Msa1272600:CDS 30.0%
TCATCAACATTATCCTTATC+GGG - chr8_3:6364776-6364795 None:intergenic 30.0%
CTCATCAACATTATCCTTAT+CGG - chr8_3:6364777-6364796 None:intergenic 30.0%
TGATGAGAATTTGAAAGAAC+AGG + chr8_3:6364790-6364809 Msa1272600:CDS 30.0%
AATTTGAAAGAACAGGATGA+TGG + chr8_3:6364797-6364816 Msa1272600:CDS 30.0%
ATCTGATGAGAATGATAATC+GGG + chr8_3:6364931-6364950 Msa1272600:CDS 30.0%
AAATTCGAGAGTCATACAAA+GGG + chr8_3:6365097-6365116 Msa1272600:CDS 30.0%
!! TTGAGAAGATGGGTTATAAA+GGG + chr8_3:6365137-6365156 Msa1272600:CDS 30.0%
TCTTGGATTTAACGAATCAA+AGG + chr8_3:6365231-6365250 Msa1272600:CDS 30.0%
CTTGGATTTAACGAATCAAA+GGG + chr8_3:6365232-6365251 Msa1272600:CDS 30.0%
CAGAAGATTTATGATATGAG+GGG + chr8_3:6365439-6365458 Msa1272600:CDS 30.0%
AGAAGATTTATGATATGAGG+GGG + chr8_3:6365440-6365459 Msa1272600:CDS 30.0%
ATAACGTAGCACTAATAGTT+CGG + chr8_3:6365551-6365570 Msa1272600:CDS 30.0%
TCAAATCTCTATCAATCTCT+TGG - chr8_3:6365594-6365613 None:intergenic 30.0%
AAGAGATTGATAGAGATTTG+AGG + chr8_3:6365593-6365612 Msa1272600:CDS 30.0%
GAAAAAAGAAAAGGAGAAGT+TGG + chr8_3:6365642-6365661 Msa1272600:CDS 30.0%
ATCCAATGTTAATGTTCCTA+AGG - chr8_3:6365752-6365771 None:intergenic 30.0%
TGGAACATTCTGATAAACAA+AGG - chr8_3:6365868-6365887 None:intergenic 30.0%
TTGTTTATCAGAATGTTCCA+AGG + chr8_3:6365868-6365887 Msa1272600:CDS 30.0%
! TCTGAAACCAATTGAGTATA+AGG - chr8_3:6365997-6366016 None:intergenic 30.0%
! TTATACTCAATTGGTTTCAG+AGG + chr8_3:6365996-6366015 Msa1272600:CDS 30.0%
! TCAATTTGAAACGAATTGTC+TGG - chr8_3:6366275-6366294 None:intergenic 30.0%
! TTCAAATTGAGTACTGTTCT+TGG + chr8_3:6366285-6366304 Msa1272600:CDS 30.0%
AATCAAATACAGTCTTCCAA+GGG - chr8_3:6366353-6366372 None:intergenic 30.0%
! TCATTTGCCAAAAGTTCTTT+TGG - chr8_3:6366645-6366664 None:intergenic 30.0%
TTACTGTTCAAACCTAAATC+TGG + chr8_3:6366918-6366937 Msa1272600:CDS 30.0%
ACAACGGTCATCAAATATAT+GGG + chr8_3:6366949-6366968 Msa1272600:CDS 30.0%
! AATCGACTCTCTAAATCAAA+AGG + chr8_3:6366992-6367011 Msa1272600:CDS 30.0%
TATAGATGAGGATCAAGAGA+TGG + chr8_3:6364511-6364530 Msa1272600:CDS 35.0%
GATCAAGAGATGGAGAATTT+CGG + chr8_3:6364521-6364540 Msa1272600:CDS 35.0%
AGAATTCACCATTAACCCAT+TGG - chr8_3:6364571-6364590 None:intergenic 35.0%
! AGCGAAGAAAAGATTTTGGA+AGG + chr8_3:6364699-6364718 Msa1272600:CDS 35.0%
CCAGTTGAAACAAAATTCAC+AGG - chr8_3:6364743-6364762 None:intergenic 35.0%
!! CCTGTGAATTTTGTTTCAAC+TGG + chr8_3:6364740-6364759 Msa1272600:CDS 35.0%
! TGGAAATTTTATGCCCGATA+AGG + chr8_3:6364760-6364779 Msa1272600:CDS 35.0%
! TCTGGTTTAGGATTTAATGC+TGG + chr8_3:6364875-6364894 Msa1272600:CDS 35.0%
! AAATTTTTGCCTACTGCGTT+TGG + chr8_3:6364959-6364978 Msa1272600:CDS 35.0%
! AATTTTTGCCTACTGCGTTT+GGG + chr8_3:6364960-6364979 Msa1272600:CDS 35.0%
GAAATTCGAGAGTCATACAA+AGG + chr8_3:6365096-6365115 Msa1272600:CDS 35.0%
!! CTTGAGAAGATGGGTTATAA+AGG + chr8_3:6365136-6365155 Msa1272600:CDS 35.0%
!! TGAGAAGATGGGTTATAAAG+GGG + chr8_3:6365138-6365157 Msa1272600:CDS 35.0%
!! GGGTATTTTGAATCCTATTG+AGG + chr8_3:6365183-6365202 Msa1272600:CDS 35.0%
AAGTTGAGGGCTAAAAATTC+TGG + chr8_3:6365208-6365227 Msa1272600:CDS 35.0%
! GGAAAAGTATGTAACTGCTA+AGG + chr8_3:6365375-6365394 Msa1272600:CDS 35.0%
! TTTGTCTGATTTGAATGCTG+AGG + chr8_3:6365486-6365505 Msa1272600:CDS 35.0%
TCTATCAATCTCTTGGATGT+TGG - chr8_3:6365587-6365606 None:intergenic 35.0%
ATAGAGATTTGAGGAAAGAG+AGG + chr8_3:6365602-6365621 Msa1272600:CDS 35.0%
TAGAGATTTGAGGAAAGAGA+GGG + chr8_3:6365603-6365622 Msa1272600:CDS 35.0%
TGAAAAGATAATGAGTGTGC+TGG + chr8_3:6365705-6365724 Msa1272600:CDS 35.0%
CACCTTAGGAACATTAACAT+TGG + chr8_3:6365747-6365766 Msa1272600:CDS 35.0%
TTATCAGAATGTTCCAAGGA+TGG + chr8_3:6365872-6365891 Msa1272600:CDS 35.0%
TATCAGAATGTTCCAAGGAT+GGG + chr8_3:6365873-6365892 Msa1272600:CDS 35.0%
GTCATCACCTTATACTCAAT+TGG + chr8_3:6365987-6366006 Msa1272600:CDS 35.0%
GAGTATCTGGTATCAATACT+TGG + chr8_3:6366037-6366056 Msa1272600:CDS 35.0%
ATAGTAGCAAGAACTGAAGA+AGG - chr8_3:6366120-6366139 None:intergenic 35.0%
ATAGGAATAGTCTCAAGATG+TGG - chr8_3:6366195-6366214 None:intergenic 35.0%
ATCAAATACAGTCTTCCAAG+GGG - chr8_3:6366352-6366371 None:intergenic 35.0%
GAATCAAATACAGTCTTCCA+AGG - chr8_3:6366354-6366373 None:intergenic 35.0%
CTGTATTTGATTCTGATAGC+TGG + chr8_3:6366361-6366380 Msa1272600:CDS 35.0%
! TCAGCTTTGGTACGATAAAA+CGG - chr8_3:6366401-6366420 None:intergenic 35.0%
! TTTTATCGTACCAAAGCTGA+AGG + chr8_3:6366401-6366420 Msa1272600:CDS 35.0%
TTACAAAATGGTATTTGGGC+TGG + chr8_3:6366607-6366626 Msa1272600:CDS 35.0%
ATTCGATACAAGCTCAATTG+TGG + chr8_3:6366669-6366688 Msa1272600:CDS 35.0%
AGGAGAAGATAAGCTATATC+AGG + chr8_3:6366748-6366767 Msa1272600:CDS 35.0%
GGAGAAGATAAGCTATATCA+GGG + chr8_3:6366749-6366768 Msa1272600:CDS 35.0%
ATCTTATTGAGGCTTATGCT+CGG + chr8_3:6366886-6366905 Msa1272600:CDS 35.0%
CACAACGGTCATCAAATATA+TGG + chr8_3:6366948-6366967 Msa1272600:CDS 35.0%
GGTCATCAAATATATGGGTT+TGG + chr8_3:6366954-6366973 Msa1272600:CDS 35.0%
AGAGATGGAGAATTTCGGAA+CGG + chr8_3:6364526-6364545 Msa1272600:CDS 40.0%
! GGAACGGAGAAAGATTTTGA+AGG + chr8_3:6364542-6364561 Msa1272600:CDS 40.0%
!! ACGGAGAAAGATTTTGAAGG+TGG + chr8_3:6364545-6364564 Msa1272600:CDS 40.0%
!! AAGATTTTGAAGGTGGCCAA+TGG + chr8_3:6364552-6364571 Msa1272600:CDS 40.0%
!! AGATTTTGAAGGTGGCCAAT+GGG + chr8_3:6364553-6364572 Msa1272600:CDS 40.0%
! TTTGAGTTCGAGCTTTCTCT+CGG - chr8_3:6364607-6364626 None:intergenic 40.0%
! AGGATGATGGTGAAGAAGAT+AGG + chr8_3:6364810-6364829 Msa1272600:CDS 40.0%
AAGAAGATAGGCCTGGTTTA+GGG + chr8_3:6364822-6364841 Msa1272600:CDS 40.0%
!! CTGGTTTAGGGTTAGGTTTT+GGG + chr8_3:6364834-6364853 Msa1272600:CDS 40.0%
!!! TTTAGGGTTAGGTTTTGGGT+CGG + chr8_3:6364838-6364857 Msa1272600:CDS 40.0%
TTAGGATTTAATGCTGGTCG+TGG + chr8_3:6364881-6364900 Msa1272600:CDS 40.0%
TGCGTTTGGGAAGATGATTA+AGG + chr8_3:6364973-6364992 Msa1272600:CDS 40.0%
ATTGGAGGAGAAGAAGAAGA+AGG + chr8_3:6365024-6365043 Msa1272600:CDS 40.0%
TTGGAGGAGAAGAAGAAGAA+GGG + chr8_3:6365025-6365044 Msa1272600:CDS 40.0%
GAGAGTCATACAAAGGGAAT+TGG + chr8_3:6365103-6365122 Msa1272600:CDS 40.0%
AATGAAGCTGCTTGAGAAGA+TGG + chr8_3:6365126-6365145 Msa1272600:CDS 40.0%
ATGAAGCTGCTTGAGAAGAT+GGG + chr8_3:6365127-6365146 Msa1272600:CDS 40.0%
!! GAGAAGATGGGTTATAAAGG+GGG + chr8_3:6365139-6365158 Msa1272600:CDS 40.0%
ATCCTATTGAGGCAAAGTTG+AGG + chr8_3:6365194-6365213 Msa1272600:CDS 40.0%
TCCTATTGAGGCAAAGTTGA+GGG + chr8_3:6365195-6365214 Msa1272600:CDS 40.0%
AGGGCTAAAAATTCTGGTCT+TGG + chr8_3:6365214-6365233 Msa1272600:CDS 40.0%
TTCTTCTCTGTCTGCAAAAC+GGG - chr8_3:6365274-6365293 None:intergenic 40.0%
CTTCTTCTCTGTCTGCAAAA+CGG - chr8_3:6365275-6365294 None:intergenic 40.0%
TGTAACTGCTAAGGAGTTGT+TGG + chr8_3:6365384-6365403 Msa1272600:CDS 40.0%
TAAGCAAGAAGAAGAGTCAG+AGG + chr8_3:6365411-6365430 Msa1272600:CDS 40.0%
TCTGTTAAAACTCGTACCTG+CGG - chr8_3:6365466-6365485 None:intergenic 40.0%
TATACTGAAGCTCAGGCATT+GGG - chr8_3:6365534-6365553 None:intergenic 40.0%
TTATACTGAAGCTCAGGCAT+TGG - chr8_3:6365535-6365554 None:intergenic 40.0%
TTCCCTTGCTCAATGCTTTA+CGG + chr8_3:6365771-6365790 Msa1272600:CDS 40.0%
!! CATGCACAAAAGATTTGCTG+AGG + chr8_3:6365795-6365814 Msa1272600:CDS 40.0%
TCGAAATCCATCTCATGGTT+TGG + chr8_3:6365903-6365922 Msa1272600:CDS 40.0%
GTATCAATACTTGGCATGCA+CGG + chr8_3:6366046-6366065 Msa1272600:CDS 40.0%
TATCAATACTTGGCATGCAC+GGG + chr8_3:6366047-6366066 Msa1272600:CDS 40.0%
!! AAAAACCGTAGCATTGGTTC+AGG - chr8_3:6366075-6366094 None:intergenic 40.0%
! TGAACCAATGCTACGGTTTT+TGG + chr8_3:6366074-6366093 Msa1272600:CDS 40.0%
CCAAAACTATCAAGTGCAGT+GGG + chr8_3:6366159-6366178 Msa1272600:CDS 40.0%
AGACTATTCCTATCCACACA+TGG + chr8_3:6366202-6366221 Msa1272600:CDS 40.0%
GACTATTCCTATCCACACAT+GGG + chr8_3:6366203-6366222 Msa1272600:CDS 40.0%
CATATGCAGAACCATCACTT+GGG - chr8_3:6366320-6366339 None:intergenic 40.0%
CATATGCTATATTGTCCCCT+TGG + chr8_3:6366334-6366353 Msa1272600:CDS 40.0%
! TGATCAAGATTCTGACTTGC+CGG - chr8_3:6366456-6366475 None:intergenic 40.0%
GGCTATTCCAATTCATCTGA+TGG + chr8_3:6366503-6366522 Msa1272600:CDS 40.0%
GTGTCAACCATCAGATGAAT+TGG - chr8_3:6366513-6366532 None:intergenic 40.0%
TCATCTGATGGTTGACACAA+TGG + chr8_3:6366515-6366534 Msa1272600:CDS 40.0%
ATACAAAACCGTAAGCCACT+TGG - chr8_3:6366553-6366572 None:intergenic 40.0%
!! GGCTTACGGTTTTGTATCAT+TGG + chr8_3:6366556-6366575 Msa1272600:CDS 40.0%
! TGGTCTTGATATGATGAACC+AGG + chr8_3:6366689-6366708 Msa1272600:CDS 40.0%
CTTATCTTCTCCTTCAAACC+AGG - chr8_3:6366741-6366760 None:intergenic 40.0%
ATATCAGGGTAAGTGAGCAA+AGG + chr8_3:6366763-6366782 Msa1272600:CDS 40.0%
GAGCGTGAAAGATCTTATTG+AGG + chr8_3:6366875-6366894 Msa1272600:CDS 40.0%
TCTTATTGAGGCTTATGCTC+GGG + chr8_3:6366887-6366906 Msa1272600:CDS 40.0%
TTGTGCGTTCTACCAGATTT+AGG - chr8_3:6366933-6366952 None:intergenic 40.0%
AAATCTGGTAGAACGCACAA+CGG + chr8_3:6366933-6366952 Msa1272600:CDS 40.0%
AAAGGTCTATGCCCAAAATG+AGG + chr8_3:6367010-6367029 Msa1272600:CDS 40.0%
! AGACCATGTCTCCTCATTTT+GGG - chr8_3:6367024-6367043 None:intergenic 40.0%
! AAGACCATGTCTCCTCATTT+TGG - chr8_3:6367025-6367044 None:intergenic 40.0%
!! TCCGAAGACGTTGATGATGA+TGG + chr8_3:6364662-6364681 Msa1272600:CDS 45.0%
CCCATCATCATCAACGTCTT+CGG - chr8_3:6364666-6364685 None:intergenic 45.0%
!! CCGAAGACGTTGATGATGAT+GGG + chr8_3:6364663-6364682 Msa1272600:CDS 45.0%
GAAGAAGATAGGCCTGGTTT+AGG + chr8_3:6364821-6364840 Msa1272600:CDS 45.0%
CCAAAACCTAACCCTAAACC+AGG - chr8_3:6364836-6364855 None:intergenic 45.0%
!! CCTGGTTTAGGGTTAGGTTT+TGG + chr8_3:6364833-6364852 Msa1272600:CDS 45.0%
!! TTTTGGGTCGGCTAGTATCT+CGG + chr8_3:6364850-6364869 Msa1272600:CDS 45.0%
ATCATCTTCCCAAACGCAGT+AGG - chr8_3:6364971-6364990 None:intergenic 45.0%
TTAAGGAGAGAGCCATGAAG+AGG + chr8_3:6364990-6365009 Msa1272600:CDS 45.0%
TAAGGAGAGAGCCATGAAGA+GGG + chr8_3:6364991-6365010 Msa1272600:CDS 45.0%
TGGAGGAGAAGAAGAAGAAG+GGG + chr8_3:6365026-6365045 Msa1272600:CDS 45.0%
!! AAGATGGGTTATAAAGGGGG+TGG + chr8_3:6365142-6365161 Msa1272600:CDS 45.0%
GTTATAAAGGGGGTGGTCTT+GGG + chr8_3:6365149-6365168 Msa1272600:CDS 45.0%
GACAGAGAAGAAGACTGAAC+CGG + chr8_3:6365282-6365301 Msa1272600:CDS 45.0%
ACAGAGAAGAAGACTGAACC+GGG + chr8_3:6365283-6365302 Msa1272600:CDS 45.0%
GAACGTGTTCTTCCAACTGT+AGG - chr8_3:6365319-6365338 None:intergenic 45.0%
GAATGCTGAGGAGAAAGCAA+AGG + chr8_3:6365498-6365517 Msa1272600:CDS 45.0%
GCTACGTTATACTGAAGCTC+AGG - chr8_3:6365541-6365560 None:intergenic 45.0%
AAAGGAGAAGTTGGAAGCTG+AGG + chr8_3:6365651-6365670 Msa1272600:CDS 45.0%
GTTGGAGAAGAGAACACCTT+AGG + chr8_3:6365733-6365752 Msa1272600:CDS 45.0%
!! GTCCGTAAAGCATTGAGCAA+GGG - chr8_3:6365776-6365795 None:intergenic 45.0%
!! TGTCCGTAAAGCATTGAGCA+AGG - chr8_3:6365777-6365796 None:intergenic 45.0%
! CCATGAGATGGATTTCGAAG+AGG - chr8_3:6365901-6365920 None:intergenic 45.0%
CCTCTTCGAAATCCATCTCA+TGG + chr8_3:6365898-6365917 Msa1272600:CDS 45.0%
! TCCATCTCATGGTTTGGAGT+TGG + chr8_3:6365909-6365928 Msa1272600:CDS 45.0%
ACCAACTCCAAACCATGAGA+TGG - chr8_3:6365913-6365932 None:intergenic 45.0%
!! GTTTGGAGTTGGTATCACAG+TGG + chr8_3:6365920-6365939 Msa1272600:CDS 45.0%
ATACCAGATACTCTGACAGC+TGG - chr8_3:6366030-6366049 None:intergenic 45.0%
GACTCCAAAAACCGTAGCAT+TGG - chr8_3:6366081-6366100 None:intergenic 45.0%
!!! TGCTACGGTTTTTGGAGTCT+TGG + chr8_3:6366082-6366101 Msa1272600:CDS 45.0%
!!! GCTACGGTTTTTGGAGTCTT+GGG + chr8_3:6366083-6366102 Msa1272600:CDS 45.0%
GCCAAAACTATCAAGTGCAG+TGG + chr8_3:6366158-6366177 Msa1272600:CDS 45.0%
!! CCCACTGCACTTGATAGTTT+TGG - chr8_3:6366162-6366181 None:intergenic 45.0%
TATCAAGTGCAGTGGGTACT+TGG + chr8_3:6366166-6366185 Msa1272600:CDS 45.0%
ATCAAGTGCAGTGGGTACTT+GGG + chr8_3:6366167-6366186 Msa1272600:CDS 45.0%
! TGAGTACTGTTCTTGGTGCA+TGG + chr8_3:6366292-6366311 Msa1272600:CDS 45.0%
GCATATGCAGAACCATCACT+TGG - chr8_3:6366321-6366340 None:intergenic 45.0%
AGATGACTTCCAAGTGAACC+CGG + chr8_3:6366434-6366453 Msa1272600:CDS 45.0%
! GATCAAGATTCTGACTTGCC+GGG - chr8_3:6366455-6366474 None:intergenic 45.0%
TTGGGTTATGAACTGGGCTT+CGG + chr8_3:6366482-6366501 Msa1272600:CDS 45.0%
TGGAGATCTTCTTTGCCAAG+TGG + chr8_3:6366535-6366554 Msa1272600:CDS 45.0%
CTTCTTTGCCAAGTGGCTTA+CGG + chr8_3:6366542-6366561 Msa1272600:CDS 45.0%
ATGATGAACCAGGCTGTTGA+AGG + chr8_3:6366699-6366718 Msa1272600:CDS 45.0%
ATGCCCAAAATGAGGAGACA+TGG + chr8_3:6367018-6367037 Msa1272600:CDS 45.0%
!! GAAGGTGGCCAATGGGTTAA+TGG + chr8_3:6364560-6364579 Msa1272600:CDS 50.0%
GATGGTGAAGAAGATAGGCC+TGG + chr8_3:6364815-6364834 Msa1272600:CDS 50.0%
! GATAGGCCTGGTTTAGGGTT+AGG + chr8_3:6364827-6364846 Msa1272600:CDS 50.0%
TTTGGGTCGGCTAGTATCTC+GGG + chr8_3:6364851-6364870 Msa1272600:CDS 50.0%
! AGTATCTCGGGGTCTGGTTT+AGG + chr8_3:6364863-6364882 Msa1272600:CDS 50.0%
AATGCTGGTCGTGGTGTGAA+TGG + chr8_3:6364890-6364909 Msa1272600:CDS 50.0%
TCTCTCCTTCTCCCTCTTCA+TGG - chr8_3:6365005-6365024 None:intergenic 50.0%
GAAGAGGGAGAAGGAGAGAT+TGG + chr8_3:6365006-6365025 Msa1272600:CDS 50.0%
GAAGAAGAAGGGGAAGAAGC+CGG + chr8_3:6365036-6365055 Msa1272600:CDS 50.0%
AAGAAGAAGGGGAAGAAGCC+GGG + chr8_3:6365037-6365056 Msa1272600:CDS 50.0%
!! AAGAAGCCGGGCATTGGTTT+AGG + chr8_3:6365049-6365068 Msa1272600:CDS 50.0%
TCCTGACCTAAACCAATGCC+CGG - chr8_3:6365058-6365077 None:intergenic 50.0%
!! GGCATTGGTTTAGGTCAGGA+GGG + chr8_3:6365058-6365077 Msa1272600:CDS 50.0%
GGTTATAAAGGGGGTGGTCT+TGG + chr8_3:6365148-6365167 Msa1272600:CDS 50.0%
! TGGTCTTGGGAAGAATGAGC+AGG + chr8_3:6365162-6365181 Msa1272600:CDS 50.0%
GGTCTTGGGAAGAATGAGCA+GGG + chr8_3:6365163-6365182 Msa1272600:CDS 50.0%
GCCCTCAACTTTGCCTCAAT+AGG - chr8_3:6365199-6365218 None:intergenic 50.0%
TCTGTCTGCAAAACGGGCAA+CGG - chr8_3:6365268-6365287 None:intergenic 50.0%
CAGAGAAGAAGACTGAACCG+GGG + chr8_3:6365284-6365303 Msa1272600:CDS 50.0%
AGAGAAGAAGACTGAACCGG+GGG + chr8_3:6365285-6365304 Msa1272600:CDS 50.0%
CAGTTGGAAGAACACGTTCG+TGG + chr8_3:6365320-6365339 Msa1272600:CDS 50.0%
ATGAGTGTGCTGGACAGAGT+TGG + chr8_3:6365715-6365734 Msa1272600:CDS 50.0%
TTCGAAGAGGATCCCATCCT+TGG - chr8_3:6365888-6365907 None:intergenic 50.0%
!! GGAGTTGGTATCACAGTGGA+AGG + chr8_3:6365924-6365943 Msa1272600:CDS 50.0%
!! CAGTGGAAGGCATTGCTTCA+AGG + chr8_3:6365937-6365956 Msa1272600:CDS 50.0%
! TTGCCAGCTGTCAGAGTATC+TGG + chr8_3:6366024-6366043 Msa1272600:CDS 50.0%
GGGATCCTGAACCAATGCTA+CGG + chr8_3:6366067-6366086 Msa1272600:CDS 50.0%
TGCAAGACAGCCTTCAGCTT+TGG - chr8_3:6366414-6366433 None:intergenic 50.0%
GAACCAGGCTGTTGAAGGTA+TGG + chr8_3:6366704-6366723 Msa1272600:CDS 50.0%
! GGTTGTGCAACCTGGTTTGA+AGG + chr8_3:6366728-6366747 Msa1272600:CDS 50.0%
!! AGCAGCTTGTTGTTGAGCAG+AGG - chr8_3:6366817-6366836 None:intergenic 50.0%
ACAACAAGCTGCTGCAAGCA+TGG + chr8_3:6366824-6366843 Msa1272600:CDS 50.0%
GAGGCTTATGCTCGGGATAA+TGG + chr8_3:6366894-6366913 Msa1272600:CDS 50.0%
TTGGGTCGGCTAGTATCTCG+GGG + chr8_3:6364852-6364871 Msa1272600:CDS 55.0%
GAGAGCCATGAAGAGGGAGA+AGG + chr8_3:6364997-6365016 Msa1272600:CDS 55.0%
GAGGGAGAAGGAGAGATTGG+AGG + chr8_3:6365009-6365028 Msa1272600:CDS 55.0%
!! GGGCATTGGTTTAGGTCAGG+AGG + chr8_3:6365057-6365076 Msa1272600:CDS 55.0%
TTTAGGTCAGGAGGGAGCTG+TGG + chr8_3:6365066-6365085 Msa1272600:CDS 55.0%
AGGAGGGAGCTGTGGATGTT+GGG + chr8_3:6365074-6365093 Msa1272600:CDS 55.0%
CAAAACGGGCAACGGCATAG+TGG - chr8_3:6365260-6365279 None:intergenic 55.0%
TTATGATATGAGGGGGCCGC+AGG + chr8_3:6365447-6365466 Msa1272600:CDS 55.0%
GGATGCACCCATGTGTGGAT+AGG - chr8_3:6366213-6366232 None:intergenic 55.0%
TCCACACATGGGTGCATCCA+TGG + chr8_3:6366214-6366233 Msa1272600:CDS 55.0%
ATCCATGGCTACCCCTGCTA+GGG + chr8_3:6366229-6366248 Msa1272600:CDS 55.0%
! TCTGACTTGCCGGGTTCACT+TGG - chr8_3:6366446-6366465 None:intergenic 55.0%
CCTCCATACCTTCAACAGCC+TGG - chr8_3:6366710-6366729 None:intergenic 55.0%
! CCAGGCTGTTGAAGGTATGG+AGG + chr8_3:6366707-6366726 Msa1272600:CDS 55.0%
GGTATGGAGGTTGTGCAACC+TGG + chr8_3:6366720-6366739 Msa1272600:CDS 55.0%
TCGGCTAGTATCTCGGGGTC+TGG + chr8_3:6364857-6364876 Msa1272600:CDS 60.0%
AAGGGGAAGAAGCCGGGCAT+TGG + chr8_3:6365043-6365062 Msa1272600:CDS 60.0%
!! GCCGGGCATTGGTTTAGGTC+AGG + chr8_3:6365054-6365073 Msa1272600:CDS 60.0%
CAGGAGGGAGCTGTGGATGT+TGG + chr8_3:6365073-6365092 Msa1272600:CDS 60.0%
ACTGTAGGCTGCACAACCCC+CGG - chr8_3:6365304-6365323 None:intergenic 60.0%
GGGGTTGTGCAGCCTACAGT+TGG + chr8_3:6365304-6365323 Msa1272600:CDS 60.0%
GCCATGGATGCACCCATGTG+TGG - chr8_3:6366218-6366237 None:intergenic 60.0%
CATCCATGGCTACCCCTGCT+AGG + chr8_3:6366228-6366247 Msa1272600:CDS 60.0%
TCCAGCTTGTGCCCTAGCAG+GGG - chr8_3:6366243-6366262 None:intergenic 60.0%
CTCCAGCTTGTGCCCTAGCA+GGG - chr8_3:6366244-6366263 None:intergenic 60.0%
GGTGCATGGCACCCAAGTGA+TGG + chr8_3:6366306-6366325 Msa1272600:CDS 60.0%
CAAGCTGCTGCAAGCATGGC+TGG + chr8_3:6366828-6366847 Msa1272600:CDS 60.0%
TGCCCTAGCAGGGGTAGCCA+TGG - chr8_3:6366234-6366253 None:intergenic 65.0%
ACCCCTGCTAGGGCACAAGC+TGG + chr8_3:6366239-6366258 Msa1272600:CDS 65.0%
CCTCCAGCTTGTGCCCTAGC+AGG - chr8_3:6366245-6366264 None:intergenic 65.0%
CCTGCTAGGGCACAAGCTGG+AGG + chr8_3:6366242-6366261 Msa1272600:CDS 65.0%
GCTGCTGCAAGCATGGCTGG+TGG + chr8_3:6366831-6366850 Msa1272600:CDS 65.0%
! AGCATGGCTGGTGGCGCAGA+TGG + chr8_3:6366840-6366859 Msa1272600:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 6364506 6367103 6364506 ID=Msa1272600;Name=Msa1272600
chr8_3 mRNA 6364506 6367103 6364506 ID=Msa1272600-mRNA-1;Parent=Msa1272600;Name=Msa1272600-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|-1|0|1|-1|1|1|0|865
chr8_3 exon 6364506 6367103 6364506 ID=Msa1272600-mRNA-1:exon:2290;Parent=Msa1272600-mRNA-1
chr8_3 CDS 6364506 6367103 6364506 ID=Msa1272600-mRNA-1:cds;Parent=Msa1272600-mRNA-1
Gene Sequence

>Msa1272600

ATGGATATAGATGAGGATCAAGAGATGGAGAATTTCGGAACGGAGAAAGATTTTGAAGGTGGCCAATGGGTTAATGGTGAATTCTACTTTCGAAAGCGCCGAGAGAAAGCTCGAACTCAAACCAAAGACGATGTTATATATGGAGTTTTTGGTGATTCCGAAGACGTTGATGATGATGGGTATTCTAGTAAAAAGCGAAGAAAAGATTTTGGAAGGAAACAGGATCTCACTAAACCTGTGAATTTTGTTTCAACTGGAAATTTTATGCCCGATAAGGATAATGTTGATGAGAATTTGAAAGAACAGGATGATGGTGAAGAAGATAGGCCTGGTTTAGGGTTAGGTTTTGGGTCGGCTAGTATCTCGGGGTCTGGTTTAGGATTTAATGCTGGTCGTGGTGTGAATGGTTCAGATAGAAATGAAAAATCTGATGAGAATGATAATCGGGATGATAAATTTTTGCCTACTGCGTTTGGGAAGATGATTAAGGAGAGAGCCATGAAGAGGGAGAAGGAGAGATTGGAGGAGAAGAAGAAGAAGGGGAAGAAGCCGGGCATTGGTTTAGGTCAGGAGGGAGCTGTGGATGTTGGGAAATTCGAGAGTCATACAAAGGGAATTGGAATGAAGCTGCTTGAGAAGATGGGTTATAAAGGGGGTGGTCTTGGGAAGAATGAGCAGGGTATTTTGAATCCTATTGAGGCAAAGTTGAGGGCTAAAAATTCTGGTCTTGGATTTAACGAATCAAAGGGAACCACTATGCCGTTGCCCGTTTTGCAGACAGAGAAGAAGACTGAACCGGGGGTTGTGCAGCCTACAGTTGGAAGAACACGTTCGTGGTTAAAGCCGCCGAAGAAAAAAAATAAAGAAGAGGAAAAGTATGTAACTGCTAAGGAGTTGTTGGCTAGTAAGCAAGAAGAAGAGTCAGAGGTTGTTCAGAAGATTTATGATATGAGGGGGCCGCAGGTACGAGTTTTAACAGATTTGTCTGATTTGAATGCTGAGGAGAAAGCAAAGGAAAATGATGTCCCAATGCCTGAGCTTCAGTATAACGTAGCACTAATAGTTCGGATAGCTGAATCCAACATCCAAGAGATTGATAGAGATTTGAGGAAAGAGAGGGATATAGCACTTAGCTTGAAAAAAGAAAAGGAGAAGTTGGAAGCTGAGGCAGCGTTTCAAAAGAAGCAGCTCGATAATTATGAAAAGATAATGAGTGTGCTGGACAGAGTTGGAGAAGAGAACACCTTAGGAACATTAACATTGGATTCCCTTGCTCAATGCTTTACGGACATGCACAAAAGATTTGCTGAGGATTATAAGCTGTGTAACTTGTCATGCATTGCTTGCTCATATGCTCTGCCTTTGTTTATCAGAATGTTCCAAGGATGGGATCCTCTTCGAAATCCATCTCATGGTTTGGAGTTGGTATCACAGTGGAAGGCATTGCTTCAAGGAGATGATTGTTTTGATATATGGGACATGTCATCACCTTATACTCAATTGGTTTCAGAGGTTGTATTGCCAGCTGTCAGAGTATCTGGTATCAATACTTGGCATGCACGGGATCCTGAACCAATGCTACGGTTTTTGGAGTCTTGGGAAAAATTGCTTCCTTCTTCAGTTCTTGCTACTATATTAGACAACATAGTCATGCCAAAACTATCAAGTGCAGTGGGTACTTGGGAACCACATCTTGAGACTATTCCTATCCACACATGGGTGCATCCATGGCTACCCCTGCTAGGGCACAAGCTGGAGGTTATCTACCAGACAATTCGTTTCAAATTGAGTACTGTTCTTGGTGCATGGCACCCAAGTGATGGTTCTGCATATGCTATATTGTCCCCTTGGAAGACTGTATTTGATTCTGATAGCTGGCAACAACTTATGCACCGTTTTATCGTACCAAAGCTGAAGGCTGTCTTGCAAGATGACTTCCAAGTGAACCCGGCAAGTCAGAATCTTGATCAATTTTATTGGGTTATGAACTGGGCTTCGGCTATTCCAATTCATCTGATGGTTGACACAATGGAGATCTTCTTTGCCAAGTGGCTTACGGTTTTGTATCATTGGTTGTGCTCAAATCCTAACTTTGATGAAGTTACAAAATGGTATTTGGGCTGGAAAGAACTTATTCCAAAAGAACTTTTGGCAAATGAAAGTATTCGATACAAGCTCAATTGTGGTCTTGATATGATGAACCAGGCTGTTGAAGGTATGGAGGTTGTGCAACCTGGTTTGAAGGAGAAGATAAGCTATATCAGGGTAAGTGAGCAAAGGAAATTTGAGACTCAGCAGAAAGCAGCAGCCTCTGCTCAACAACAAGCTGCTGCAAGCATGGCTGGTGGCGCAGATGGTGTGAATGAAATGAGCGTGAAAGATCTTATTGAGGCTTATGCTCGGGATAATGGTTTACTGTTCAAACCTAAATCTGGTAGAACGCACAACGGTCATCAAATATATGGGTTTGGCAATGTCAGCATAATAATCGACTCTCTAAATCAAAAGGTCTATGCCCAAAATGAGGAGACATGGTCTTTAGAAACTCTTGAACGATTGCTAGAGTTACATAATAAATCCCTTAGTAAAAGACGCTGA
Protein sequence

>Msa1272600

MDIDEDQEMENFGTEKDFEGGQWVNGEFYFRKRREKARTQTKDDVIYGVFGDSEDVDDDGYSSKKRRKDFGRKQDLTKPVNFVSTGNFMPDKDNVDENLKEQDDGEEDRPGLGLGFGSASISGSGLGFNAGRGVNGSDRNEKSDENDNRDDKFLPTAFGKMIKERAMKREKERLEEKKKKGKKPGIGLGQEGAVDVGKFESHTKGIGMKLLEKMGYKGGGLGKNEQGILNPIEAKLRAKNSGLGFNESKGTTMPLPVLQTEKKTEPGVVQPTVGRTRSWLKPPKKKNKEEEKYVTAKELLASKQEEESEVVQKIYDMRGPQVRVLTDLSDLNAEEKAKENDVPMPELQYNVALIVRIAESNIQEIDRDLRKERDIALSLKKEKEKLEAEAAFQKKQLDNYEKIMSVLDRVGEENTLGTLTLDSLAQCFTDMHKRFAEDYKLCNLSCIACSYALPLFIRMFQGWDPLRNPSHGLELVSQWKALLQGDDCFDIWDMSSPYTQLVSEVVLPAVRVSGINTWHARDPEPMLRFLESWEKLLPSSVLATILDNIVMPKLSSAVGTWEPHLETIPIHTWVHPWLPLLGHKLEVIYQTIRFKLSTVLGAWHPSDGSAYAILSPWKTVFDSDSWQQLMHRFIVPKLKAVLQDDFQVNPASQNLDQFYWVMNWASAIPIHLMVDTMEIFFAKWLTVLYHWLCSNPNFDEVTKWYLGWKELIPKELLANESIRYKLNCGLDMMNQAVEGMEVVQPGLKEKISYIRVSEQRKFETQQKAAASAQQQAAASMAGGADGVNEMSVKDLIEAYARDNGLLFKPKSGRTHNGHQIYGFGNVSIIIDSLNQKVYAQNEETWSLETLERLLELHNKSLSKRR*