AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1230040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1230040 MtrunA17_Chr8g0337821 96.194 867 31 2 1 866 1 866 0.0 1724
Msa1230040 MtrunA17_Chr7g0243471 77.576 495 101 4 379 866 84 575 0.0 760
Msa1230040 MtrunA17_Chr7g0243471 58.537 123 37 3 188 309 15 124 5.10e-31 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1230040 AT1G17070.1 53.681 883 345 16 3 861 1 843 0.0 832
Msa1230040 AT2G42330.2 48.514 841 331 15 34 866 6 752 0.0 722
Msa1230040 AT2G42330.1 48.514 841 331 15 34 866 6 752 0.0 722
Msa1230040 AT2G42330.3 48.514 841 331 15 34 866 6 752 0.0 722

Find 216 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACCATGTCTCCTCATTT+TGG 0.120770 8_2:-6005064 None:intergenic
TGAAGTTACAAAATGGTATT+TGG 0.125933 8_2:+6004644 Msa1230040:CDS
CCTGGTTTAGGGTTAGGTTT+TGG 0.151973 8_2:+6002872 Msa1230040:CDS
CACAACGGTCATCAAATATA+TGG 0.248012 8_2:+6004990 Msa1230040:CDS
TCCATATATAACATCGTCTT+TGG 0.271281 8_2:-6002666 None:intergenic
TCATTTGCCAAAAGTTCTTT+TGG 0.273610 8_2:-6004684 None:intergenic
TTCAAATTGAGTACTGTTCT+TGG 0.275822 8_2:+6004327 Msa1230040:CDS
CTCATCAACATTATCCTTAT+CGG 0.278789 8_2:-6002813 None:intergenic
CCCACTGCACTTGATAGTTT+TGG 0.287889 8_2:-6004201 None:intergenic
GAAGGTGGTCAATGGGTTAA+TGG 0.291147 8_2:+6002599 Msa1230040:CDS
TTCCCTTGCTCAATGCTTTA+CGG 0.291345 8_2:+6003813 Msa1230040:CDS
GAAGAAGATAGGCCTGGTTT+AGG 0.292810 8_2:+6002860 Msa1230040:CDS
AAGAAGCCGGGCATTGGTTT+AGG 0.293073 8_2:+6003091 Msa1230040:CDS
TCTGAAACCAATTGAGTATA+AGG 0.301102 8_2:-6004036 None:intergenic
ACTTATTCCAAAAGAACTTT+TGG 0.305783 8_2:+6004677 Msa1230040:CDS
AGTATCTCGGGGTCTGGTTT+AGG 0.309623 8_2:+6002902 Msa1230040:CDS
TTGTGCGTTCTACCAGATTT+AGG 0.311130 8_2:-6004972 None:intergenic
GAAGAGGGAGAAGGAGAGTT+TGG 0.314429 8_2:+6003045 Msa1230040:CDS
ACTCTCCTTCTCCCTCTTCA+TGG 0.316093 8_2:-6003041 None:intergenic
ACCAAAGACGATGTTATATA+TGG 0.321878 8_2:+6002665 Msa1230040:CDS
GAGGCTTATGCTCGGGATAA+TGG 0.323040 8_2:+6004936 Msa1230040:CDS
TTTGTAACTTCATCAAAGTT+AGG 0.326962 8_2:-6004633 None:intergenic
TTGCCAGCTGTCAGAGTATC+TGG 0.339471 8_2:+6004066 Msa1230040:CDS
TTCTTCTCTGTCTGCAAAAC+GGG 0.347374 8_2:-6003313 None:intergenic
CTTGAGAAGATGGGTTATAA+AGG 0.348414 8_2:+6003178 Msa1230040:CDS
AGGAGAAGATAAGCTATATC+AGG 0.352380 8_2:+6004790 Msa1230040:CDS
TTTGGGTCGGCTAGTATCTC+GGG 0.355857 8_2:+6002890 Msa1230040:CDS
TGATCAAGATTCTGACTTGC+CGG 0.356856 8_2:-6004495 None:intergenic
ACTTTGATGAAGTTACAAAA+TGG 0.363094 8_2:+6004637 Msa1230040:CDS
TTACTGTTCAAACCTAAATC+TGG 0.369481 8_2:+6004960 Msa1230040:CDS
GTCATCACCTTATACTCAAT+TGG 0.369878 8_2:+6004029 Msa1230040:CDS
CCCATCATCATCGTAGTCTT+CGG 0.369989 8_2:-6002702 None:intergenic
TATCAAGTGCAGTGGGTACT+TGG 0.373265 8_2:+6004208 Msa1230040:CDS
TCGAAATCCATCTCATGGTT+TGG 0.376763 8_2:+6003945 Msa1230040:CDS
CAAGCTGCTGCAAGCATGGC+TGG 0.379886 8_2:+6004870 Msa1230040:CDS
CTGGGTTATGAACTGGGCTT+CGG 0.381620 8_2:+6004524 Msa1230040:CDS
TGCAAGACAGCCTTCAGCTT+TGG 0.383113 8_2:-6004453 None:intergenic
TTCAGAAGATTTATGATATG+AGG 0.386976 8_2:+6003479 Msa1230040:CDS
GATAGGCCTGGTTTAGGGTT+AGG 0.390604 8_2:+6002866 Msa1230040:CDS
GAGAGTCATACAAAGGGAAT+TGG 0.392137 8_2:+6003145 Msa1230040:CDS
AAGAAGATAGGCCTGGTTTA+GGG 0.393008 8_2:+6002861 Msa1230040:CDS
AATCCGATGAGAATGATAAT+CGG 0.400720 8_2:+6002969 Msa1230040:CDS
CATCCATGGCTACCCCTGCT+AGG 0.404541 8_2:+6004270 Msa1230040:CDS
GCCCTCAACTTTGCCTCAAT+AGG 0.405496 8_2:-6003238 None:intergenic
GTGTCAACCATCAGATGAAT+TGG 0.408091 8_2:-6004552 None:intergenic
AGGAGGGAGCTGTGGATGTT+GGG 0.409566 8_2:+6003116 Msa1230040:CDS
CCATCATCATCGTAGTCTTC+GGG 0.411589 8_2:-6002701 None:intergenic
GATCAAGAGATGGAGAATTT+CGG 0.416250 8_2:+6002560 Msa1230040:CDS
CTTCTTTGCCAAGTGGCTTA+CGG 0.416957 8_2:+6004584 Msa1230040:CDS
GAAGAAGAAGGGGAAGAAGC+CGG 0.421415 8_2:+6003078 Msa1230040:CDS
TCAAATCTCTATCAATCTCT+TGG 0.426322 8_2:-6003633 None:intergenic
CCTCCAGCTTGTGCCCTAGC+AGG 0.432848 8_2:-6004284 None:intergenic
TTCGAAGAGGATCCCATCCT+TGG 0.432902 8_2:-6003927 None:intergenic
GGTCATCAAATATATGGGTT+TGG 0.434302 8_2:+6004996 Msa1230040:CDS
TTGAGAAGATGGGTTATAAA+GGG 0.445982 8_2:+6003179 Msa1230040:CDS
TCAATTTGAAACGAATTGTC+TGG 0.447524 8_2:-6004314 None:intergenic
TCATCAACATTATCCTTATC+GGG 0.448438 8_2:-6002812 None:intergenic
GGCTATTCCAATTCATCTGA+TGG 0.452495 8_2:+6004545 Msa1230040:CDS
TGCGTTTGGGAAGAAGATTA+AGG 0.457477 8_2:+6003012 Msa1230040:CDS
CAGGAGGGAGCTGTGGATGT+TGG 0.458207 8_2:+6003115 Msa1230040:CDS
CTTCTTCTCTGTCTGCAAAA+CGG 0.458538 8_2:-6003314 None:intergenic
CAGTGGAAGGCATTGCTTCA+AGG 0.460014 8_2:+6003979 Msa1230040:CDS
TTACAAAATGGTATTTGGGC+TGG 0.461685 8_2:+6004649 Msa1230040:CDS
AGGATGATGGTGAAGAAGAT+AGG 0.462708 8_2:+6002849 Msa1230040:CDS
GCCGGGCATTGGTTTAGGTC+AGG 0.463580 8_2:+6003096 Msa1230040:CDS
GACAGAGAAGAAGACTGAAC+CGG 0.467128 8_2:+6003324 Msa1230040:CDS
CCAGTTGAAACAAAATTCAC+AGG 0.467449 8_2:-6002779 None:intergenic
TGATGAGAATTTGAAAGAAC+AGG 0.467505 8_2:+6002829 Msa1230040:CDS
TCTATCAATCTCTTGGATGT+TGG 0.469174 8_2:-6003626 None:intergenic
TGTAACTGCTAAGGAGTTGT+TGG 0.469240 8_2:+6003426 Msa1230040:CDS
GAAGTTACAAAATGGTATTT+GGG 0.470045 8_2:+6004645 Msa1230040:CDS
AATCGACTCTCTAAATCAAA+AGG 0.472047 8_2:+6005034 Msa1230040:CDS
TCGGCTAGTATCTCGGGGTC+TGG 0.472352 8_2:+6002896 Msa1230040:CDS
TCTGACTTGCCGGGTTCACT+TGG 0.475554 8_2:-6004485 None:intergenic
ATCCGATGAGAATGATAATC+GGG 0.476029 8_2:+6002970 Msa1230040:CDS
AATGAAGCTGCTTGAGAAGA+TGG 0.478922 8_2:+6003168 Msa1230040:CDS
GTTGGAGAAGAGAACACCTT+AGG 0.479578 8_2:+6003775 Msa1230040:CDS
AAGAAGAAGGGGAAGAAGCC+GGG 0.485235 8_2:+6003079 Msa1230040:CDS
CATATGCTATATTGTCCCCT+TGG 0.485482 8_2:+6004376 Msa1230040:CDS
AGCATGGCTGGTGGCGCAGA+TGG 0.487215 8_2:+6004882 Msa1230040:CDS
ATCCATGGCTACCCCTGCTA+GGG 0.489307 8_2:+6004271 Msa1230040:CDS
TTTGAGTTTGAGCTTTCTCT+CGG 0.489770 8_2:-6002643 None:intergenic
AAGGGGAAGAAGCCGGGCAT+TGG 0.491690 8_2:+6003085 Msa1230040:CDS
TATACTGAAGCTCAGGCATT+GGG 0.492735 8_2:-6003573 None:intergenic
CCCGAAGACTACGATGATGA+TGG 0.493497 8_2:+6002701 Msa1230040:CDS
TGCCCTAGCAGGGGTAGCCA+TGG 0.494447 8_2:-6004273 None:intergenic
CTTATCTTCTCCTTCAAACC+AGG 0.494860 8_2:-6004780 None:intergenic
ACCCCTGCTAGGGCACAAGC+TGG 0.496535 8_2:+6004281 Msa1230040:CDS
CTGTATTTGATTCTGATAGC+TGG 0.496901 8_2:+6004403 Msa1230040:CDS
GATCAAGATTCTGACTTGCC+GGG 0.497039 8_2:-6004494 None:intergenic
TTATACTGAAGCTCAGGCAT+TGG 0.497443 8_2:-6003574 None:intergenic
ATCCAATGTTAATGTTCCTA+AGG 0.499131 8_2:-6003791 None:intergenic
TCAGCTTTGGTACGATAAAA+CGG 0.499436 8_2:-6004440 None:intergenic
AAGAGATTGATAGAGATTTG+AGG 0.504347 8_2:+6003635 Msa1230040:CDS
CACCTTAGGAACATTAACAT+TGG 0.505825 8_2:+6003789 Msa1230040:CDS
TCCTATTGAGGCAAAGTTGA+GGG 0.506308 8_2:+6003237 Msa1230040:CDS
ATAACGTAGCACTAATAGTT+CGG 0.507572 8_2:+6003593 Msa1230040:CDS
ATCTTATTGAGGCTTATGCT+CGG 0.511086 8_2:+6004928 Msa1230040:CDS
ATAGAGATTTGAGGAAAGAG+AGG 0.513041 8_2:+6003644 Msa1230040:CDS
GCCAAAACTATCAAGTGCAG+TGG 0.513710 8_2:+6004200 Msa1230040:CDS
GTATCAATACTTGGCATGCA+CGG 0.515579 8_2:+6004088 Msa1230040:CDS
ACAACGGTCATCAAATATAT+GGG 0.518474 8_2:+6004991 Msa1230040:CDS
ACAACAAGCTGCTGCAAGCA+TGG 0.518724 8_2:+6004866 Msa1230040:CDS
GAGGGAGAAGGAGAGTTTGG+AGG 0.520940 8_2:+6003048 Msa1230040:CDS
TCCACACATGGGTGCATCCA+TGG 0.522047 8_2:+6004256 Msa1230040:CDS
TCAGAAGATTTATGATATGA+GGG 0.525953 8_2:+6003480 Msa1230040:CDS
TCTTATTGAGGCTTATGCTC+GGG 0.526021 8_2:+6004929 Msa1230040:CDS
TCCATCTCATGGTTTGGAGT+TGG 0.526127 8_2:+6003951 Msa1230040:CDS
GGTTGTGCAACCTGGTTTGA+AGG 0.528723 8_2:+6004770 Msa1230040:CDS
GCTACGTTATACTGAAGCTC+AGG 0.531815 8_2:-6003580 None:intergenic
GAACCAGGCTGTTGAAGGTA+TGG 0.534382 8_2:+6004746 Msa1230040:CDS
ATAGTAGCAAGAACTGAAGA+AGG 0.534632 8_2:-6004159 None:intergenic
TTTAGGTCAGGAGGGAGCTG+TGG 0.534834 8_2:+6003108 Msa1230040:CDS
AATGCTGGTCGTGGTGTGAA+TGG 0.539364 8_2:+6002929 Msa1230040:CDS
TATCAGAATGTTCCAAGGAT+GGG 0.541188 8_2:+6003915 Msa1230040:CDS
CAGAAGATTTATGATATGAG+GGG 0.544424 8_2:+6003481 Msa1230040:CDS
GGAAAAGTATGTAACTGCTA+AGG 0.545075 8_2:+6003417 Msa1230040:CDS
TTATCAGAATGTTCCAAGGA+TGG 0.545083 8_2:+6003914 Msa1230040:CDS
TATCAATACTTGGCATGCAC+GGG 0.546744 8_2:+6004089 Msa1230040:CDS
TCTGGTTTAGGATTTAATGC+TGG 0.547046 8_2:+6002914 Msa1230040:CDS
CCTCTTCGAAATCCATCTCA+TGG 0.547824 8_2:+6003940 Msa1230040:CDS
CTCCAGCTTGTGCCCTAGCA+GGG 0.548394 8_2:-6004283 None:intergenic
TGAAAAGATAATGAGTGTGC+TGG 0.550040 8_2:+6003747 Msa1230040:CDS
GGTATGGAGGTTGTGCAACC+TGG 0.550151 8_2:+6004762 Msa1230040:CDS
TCATCTGATGGTTGACACAA+TGG 0.550438 8_2:+6004557 Msa1230040:CDS
TGGTCTTGGGAAGAATGAGC+AGG 0.554574 8_2:+6003204 Msa1230040:CDS
TTATACTCAATTGGTTTCAG+AGG 0.556724 8_2:+6004038 Msa1230040:CDS
ATCAAGTGCAGTGGGTACTT+GGG 0.558150 8_2:+6004209 Msa1230040:CDS
ACAGAGAAGAAGACTGAACC+GGG 0.558425 8_2:+6003325 Msa1230040:CDS
AGCAGCTTGTTGTTGAGCAG+AGG 0.559345 8_2:-6004856 None:intergenic
CAAAACGGGCAACGGCATAG+TGG 0.559472 8_2:-6003299 None:intergenic
GAGTATCTGGTATCAATACT+TGG 0.559939 8_2:+6004079 Msa1230040:CDS
TATAGATGAGGATCAAGAGA+TGG 0.560053 8_2:+6002550 Msa1230040:CDS
GCTGCTGCAAGCATGGCTGG+TGG 0.561489 8_2:+6004873 Msa1230040:CDS
ACTGTAGGCTGCACAACCCC+CGG 0.561582 8_2:-6003343 None:intergenic
GCATATGCAGAACCATCACT+TGG 0.561871 8_2:-6004360 None:intergenic
GGGAGCCATGAAGAGGGAGA+AGG 0.561897 8_2:+6003036 Msa1230040:CDS
GAATCAAATACAGTCTTCCA+AGG 0.562070 8_2:-6004393 None:intergenic
GGAGTTGGTATCACAGTGGA+AGG 0.562462 8_2:+6003966 Msa1230040:CDS
ATCCCGATTATCATTCTCAT+CGG 0.562686 8_2:-6002972 None:intergenic
AAAGGAGAAGTTGGAAGCTG+AGG 0.562740 8_2:+6003693 Msa1230040:CDS
TTAGGATTTAATGCTGGTCG+TGG 0.567508 8_2:+6002920 Msa1230040:CDS
AGATGACTTCCAAGTGAACC+CGG 0.567771 8_2:+6004476 Msa1230040:CDS
GGTGCATGGCACCCAAGTGA+TGG 0.568932 8_2:+6004348 Msa1230040:CDS
CCGAAGACTACGATGATGAT+GGG 0.576988 8_2:+6002702 Msa1230040:CDS
ATGATGAACCAGGCTGTTGA+AGG 0.577293 8_2:+6004741 Msa1230040:CDS
GGGGTTGTGCAGCCTACAGT+TGG 0.578126 8_2:+6003346 Msa1230040:CDS
TAAGCAAGAAGAAGAGTCAG+AGG 0.579514 8_2:+6003453 Msa1230040:CDS
ATACAAAACCGTAAGCCACT+TGG 0.580893 8_2:-6004592 None:intergenic
CCTCCATACCTTCAACAGCC+TGG 0.581127 8_2:-6004749 None:intergenic
GAGCGTGAAAGATCTTATTG+AGG 0.582014 8_2:+6004917 Msa1230040:CDS
AAAGGTCTATGCCCAAAATG+AGG 0.582372 8_2:+6005052 Msa1230040:CDS
TAGAGATTTGAGGAAAGAGA+GGG 0.583409 8_2:+6003645 Msa1230040:CDS
ACCAACTCCAAACCATGAGA+TGG 0.585318 8_2:-6003952 None:intergenic
TGAGAAGATGGGTTATAAAG+GGG 0.586441 8_2:+6003180 Msa1230040:CDS
TCTGTCTGCAAAACGGGCAA+CGG 0.587541 8_2:-6003307 None:intergenic
TCTTGGATTTAACGAATCGA+AGG 0.588690 8_2:+6003273 Msa1230040:CDS
AAATTCGAGAGTCATACAAA+GGG 0.591053 8_2:+6003139 Msa1230040:CDS
TTGTTTATCAGAATGTTCCA+AGG 0.593879 8_2:+6003910 Msa1230040:CDS
ATCCTATTGAGGCAAAGTTG+AGG 0.595316 8_2:+6003236 Msa1230040:CDS
ATACCAGATACTCTGACAGC+TGG 0.595723 8_2:-6004069 None:intergenic
CAGTTGGAAGAACACGTTCG+TGG 0.598877 8_2:+6003362 Msa1230040:CDS
GAACGTGTTCTTCCAACTGT+AGG 0.599260 8_2:-6003358 None:intergenic
GAAATTCGAGAGTCATACAA+AGG 0.599821 8_2:+6003138 Msa1230040:CDS
TAAGGAGGGAGCCATGAAGA+GGG 0.600103 8_2:+6003030 Msa1230040:CDS
GATGGTGAAGAAGATAGGCC+TGG 0.601036 8_2:+6002854 Msa1230040:CDS
GGCATTGGTTTAGGTCAGGA+GGG 0.601669 8_2:+6003100 Msa1230040:CDS
GGAGAAGATAAGCTATATCA+GGG 0.605602 8_2:+6004791 Msa1230040:CDS
AATCAAATACAGTCTTCCAA+GGG 0.606618 8_2:-6004392 None:intergenic
ATTCGATACAAGCTCAATTG+TGG 0.607064 8_2:+6004711 Msa1230040:CDS
TTAGAAGATGGATATAGATG+AGG 0.607275 8_2:+6002538 None:intergenic
TGGAGATCTTCTTTGCCAAG+TGG 0.608455 8_2:+6004577 Msa1230040:CDS
GGATGCACCCATGTGTGGAT+AGG 0.608809 8_2:-6004252 None:intergenic
ATCTTCTTCCCAAACGCAGT+AGG 0.610094 8_2:-6003007 None:intergenic
TCCTGACCTAAACCAATGCC+CGG 0.610190 8_2:-6003097 None:intergenic
ATGAAGCTGCTTGAGAAGAT+GGG 0.610235 8_2:+6003169 Msa1230040:CDS
CTTGGATTTAACGAATCGAA+GGG 0.614678 8_2:+6003274 Msa1230040:CDS
TCCAGCTTGTGCCCTAGCAG+GGG 0.617024 8_2:-6004282 None:intergenic
TGGAACATTCTGATAAACAA+AGG 0.617370 8_2:-6003907 None:intergenic
AGACTATTCCTATCCACACA+TGG 0.617573 8_2:+6004244 Msa1230040:CDS
GCCATGGATGCACCCATGTG+TGG 0.620709 8_2:-6004257 None:intergenic
TTTGTCTGATTTGAATGCTG+AGG 0.621521 8_2:+6003528 Msa1230040:CDS
GGGCATTGGTTTAGGTCAGG+AGG 0.622016 8_2:+6003099 Msa1230040:CDS
TTAAGGAGGGAGCCATGAAG+AGG 0.623072 8_2:+6003029 Msa1230040:CDS
ATGCCCAAAATGAGGAGACA+TGG 0.624654 8_2:+6005060 Msa1230040:CDS
TGTCCGTAAAGCATTGAGCA+AGG 0.624936 8_2:-6003816 None:intergenic
CCAAAACCTAACCCTAAACC+AGG 0.625240 8_2:-6002872 None:intergenic
TTTGGGAAGAAGATTAAGGA+GGG 0.625461 8_2:+6003016 Msa1230040:CDS
ATATCAGGGTAAGTGAGCAA+AGG 0.626469 8_2:+6004805 Msa1230040:CDS
GAGAAGATGGGTTATAAAGG+GGG 0.630333 8_2:+6003181 Msa1230040:CDS
GTCCGTAAAGCATTGAGCAA+GGG 0.632161 8_2:-6003815 None:intergenic
TGAGTACTGTTCTTGGTGCA+TGG 0.632481 8_2:+6004334 Msa1230040:CDS
GAATGCTGAGGAGAAAGCAA+AGG 0.632682 8_2:+6003540 Msa1230040:CDS
CATGCACAAAAGATTTGCTG+AGG 0.634301 8_2:+6003837 Msa1230040:CDS
AATTTGAAAGAACAGGATGA+TGG 0.635084 8_2:+6002836 Msa1230040:CDS
GGGATCCTGAACCAATGCTA+CGG 0.635248 8_2:+6004109 Msa1230040:CDS
CCATGAGATGGATTTCGAAG+AGG 0.636233 8_2:-6003940 None:intergenic
ATGAGTGTGCTGGACAGAGT+TGG 0.636674 8_2:+6003757 Msa1230040:CDS
AGAAGATTTATGATATGAGG+GGG 0.637702 8_2:+6003482 Msa1230040:CDS
GTTTGGGAAGAAGATTAAGG+AGG 0.643771 8_2:+6003015 Msa1230040:CDS
CCAAAACTATCAAGTGCAGT+GGG 0.645728 8_2:+6004201 Msa1230040:CDS
TTGGGTCGGCTAGTATCTCG+GGG 0.651866 8_2:+6002891 Msa1230040:CDS
TCTGTTAAAACTCGTACCTG+CGG 0.658982 8_2:-6003505 None:intergenic
GACTATTCCTATCCACACAT+GGG 0.664132 8_2:+6004245 Msa1230040:CDS
CCAGGCTGTTGAAGGTATGG+AGG 0.671559 8_2:+6004749 Msa1230040:CDS
AGAGATGGAGAATTTCGGAA+CGG 0.680307 8_2:+6002565 Msa1230040:CDS
ATAGGAATAGTCTCAAGATG+TGG 0.683050 8_2:-6004234 None:intergenic
GGTCTTGGGAAGAATGAGCA+GGG 0.684826 8_2:+6003205 Msa1230040:CDS
CATATGCAGAACCATCACTT+GGG 0.687563 8_2:-6004359 None:intergenic
ATCAAATACAGTCTTCCAAG+GGG 0.688889 8_2:-6004391 None:intergenic
CCTGCTAGGGCACAAGCTGG+AGG 0.690416 8_2:+6004284 Msa1230040:CDS
TGGTCTTGATATGATGAACC+AGG 0.696080 8_2:+6004731 Msa1230040:CDS
CAGAGAAGAAGACTGAACCG+GGG 0.707743 8_2:+6003326 Msa1230040:CDS
AGAGAAGAAGACTGAACCGG+GGG 0.713315 8_2:+6003327 Msa1230040:CDS
GTTTGGAGTTGGTATCACAG+TGG 0.727402 8_2:+6003962 Msa1230040:CDS
AAATCTGGTAGAACGCACAA+CGG 0.732054 8_2:+6004975 Msa1230040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTCTTTATTTTTTTTCTT+CGG - chr8_2:6003397-6003416 None:intergenic 15.0%
!! GAAGAAAAAAAATAAAGAAG+AGG + chr8_2:6003396-6003415 Msa1230040:CDS 20.0%
!!! AGATGATTGTTTTGATATAT+GGG + chr8_2:6004002-6004021 Msa1230040:CDS 20.0%
!! GATGTTATATATGGAGTTTT+TGG + chr8_2:6002674-6002693 Msa1230040:CDS 25.0%
!! AAAAAGCGAAGAAAAGATTT+TGG + chr8_2:6002734-6002753 Msa1230040:CDS 25.0%
!!! TCTTTATTTTTTTTCTTCGG+CGG - chr8_2:6003394-6003413 None:intergenic 25.0%
! TTCAGAAGATTTATGATATG+AGG + chr8_2:6003479-6003498 Msa1230040:CDS 25.0%
! TCAGAAGATTTATGATATGA+GGG + chr8_2:6003480-6003499 Msa1230040:CDS 25.0%
!! ACTTAGCTTGAAAAAAGAAA+AGG + chr8_2:6003675-6003694 Msa1230040:CDS 25.0%
!!! GAGATGATTGTTTTGATATA+TGG + chr8_2:6004001-6004020 Msa1230040:CDS 25.0%
!! AGAATCTTGATCAATTTTAC+TGG + chr8_2:6004505-6004524 Msa1230040:CDS 25.0%
!! GAATCTTGATCAATTTTACT+GGG + chr8_2:6004506-6004525 Msa1230040:CDS 25.0%
! TTTGTAACTTCATCAAAGTT+AGG - chr8_2:6004636-6004655 None:intergenic 25.0%
! ACTTTGATGAAGTTACAAAA+TGG + chr8_2:6004637-6004656 Msa1230040:CDS 25.0%
! TGAAGTTACAAAATGGTATT+TGG + chr8_2:6004644-6004663 Msa1230040:CDS 25.0%
! GAAGTTACAAAATGGTATTT+GGG + chr8_2:6004645-6004664 Msa1230040:CDS 25.0%
!! ACTTATTCCAAAAGAACTTT+TGG + chr8_2:6004677-6004696 Msa1230040:CDS 25.0%
ACCAAAGACGATGTTATATA+TGG + chr8_2:6002665-6002684 Msa1230040:CDS 30.0%
TCCATATATAACATCGTCTT+TGG - chr8_2:6002669-6002688 None:intergenic 30.0%
!! AAAAGATTTTGGAAGGAAAC+AGG + chr8_2:6002745-6002764 Msa1230040:CDS 30.0%
TCATCAACATTATCCTTATC+GGG - chr8_2:6002815-6002834 None:intergenic 30.0%
CTCATCAACATTATCCTTAT+CGG - chr8_2:6002816-6002835 None:intergenic 30.0%
TGATGAGAATTTGAAAGAAC+AGG + chr8_2:6002829-6002848 Msa1230040:CDS 30.0%
AATTTGAAAGAACAGGATGA+TGG + chr8_2:6002836-6002855 Msa1230040:CDS 30.0%
AATCCGATGAGAATGATAAT+CGG + chr8_2:6002969-6002988 Msa1230040:CDS 30.0%
AAATTCGAGAGTCATACAAA+GGG + chr8_2:6003139-6003158 Msa1230040:CDS 30.0%
!! TTGAGAAGATGGGTTATAAA+GGG + chr8_2:6003179-6003198 Msa1230040:CDS 30.0%
CAGAAGATTTATGATATGAG+GGG + chr8_2:6003481-6003500 Msa1230040:CDS 30.0%
AGAAGATTTATGATATGAGG+GGG + chr8_2:6003482-6003501 Msa1230040:CDS 30.0%
ATAACGTAGCACTAATAGTT+CGG + chr8_2:6003593-6003612 Msa1230040:CDS 30.0%
TCAAATCTCTATCAATCTCT+TGG - chr8_2:6003636-6003655 None:intergenic 30.0%
AAGAGATTGATAGAGATTTG+AGG + chr8_2:6003635-6003654 Msa1230040:CDS 30.0%
GAAAAAAGAAAAGGAGAAGT+TGG + chr8_2:6003684-6003703 Msa1230040:CDS 30.0%
ATCCAATGTTAATGTTCCTA+AGG - chr8_2:6003794-6003813 None:intergenic 30.0%
TGGAACATTCTGATAAACAA+AGG - chr8_2:6003910-6003929 None:intergenic 30.0%
TTGTTTATCAGAATGTTCCA+AGG + chr8_2:6003910-6003929 Msa1230040:CDS 30.0%
! TCTGAAACCAATTGAGTATA+AGG - chr8_2:6004039-6004058 None:intergenic 30.0%
! TTATACTCAATTGGTTTCAG+AGG + chr8_2:6004038-6004057 Msa1230040:CDS 30.0%
! TCAATTTGAAACGAATTGTC+TGG - chr8_2:6004317-6004336 None:intergenic 30.0%
! TTCAAATTGAGTACTGTTCT+TGG + chr8_2:6004327-6004346 Msa1230040:CDS 30.0%
AATCAAATACAGTCTTCCAA+GGG - chr8_2:6004395-6004414 None:intergenic 30.0%
! AATTTTACTGGGTTATGAAC+TGG + chr8_2:6004517-6004536 Msa1230040:CDS 30.0%
! ATTTTACTGGGTTATGAACT+GGG + chr8_2:6004518-6004537 Msa1230040:CDS 30.0%
! TCATTTGCCAAAAGTTCTTT+TGG - chr8_2:6004687-6004706 None:intergenic 30.0%
TTACTGTTCAAACCTAAATC+TGG + chr8_2:6004960-6004979 Msa1230040:CDS 30.0%
ACAACGGTCATCAAATATAT+GGG + chr8_2:6004991-6005010 Msa1230040:CDS 30.0%
! AATCGACTCTCTAAATCAAA+AGG + chr8_2:6005034-6005053 Msa1230040:CDS 30.0%
TATAGATGAGGATCAAGAGA+TGG + chr8_2:6002550-6002569 Msa1230040:CDS 35.0%
GATCAAGAGATGGAGAATTT+CGG + chr8_2:6002560-6002579 Msa1230040:CDS 35.0%
!! AAGATTTTGAAGGTGGTCAA+TGG + chr8_2:6002591-6002610 Msa1230040:CDS 35.0%
!! AGATTTTGAAGGTGGTCAAT+GGG + chr8_2:6002592-6002611 Msa1230040:CDS 35.0%
! TTTGAGTTTGAGCTTTCTCT+CGG - chr8_2:6002646-6002665 None:intergenic 35.0%
! AGCGAAGAAAAGATTTTGGA+AGG + chr8_2:6002738-6002757 Msa1230040:CDS 35.0%
CCAGTTGAAACAAAATTCAC+AGG - chr8_2:6002782-6002801 None:intergenic 35.0%
!! CCTGTGAATTTTGTTTCAAC+TGG + chr8_2:6002779-6002798 Msa1230040:CDS 35.0%
! TGGAAATTTTATGCCCGATA+AGG + chr8_2:6002799-6002818 Msa1230040:CDS 35.0%
! TCTGGTTTAGGATTTAATGC+TGG + chr8_2:6002914-6002933 Msa1230040:CDS 35.0%
ATCCGATGAGAATGATAATC+GGG + chr8_2:6002970-6002989 Msa1230040:CDS 35.0%
ATCCCGATTATCATTCTCAT+CGG - chr8_2:6002975-6002994 None:intergenic 35.0%
! AAATTTTTGCCTACTGCGTT+TGG + chr8_2:6002998-6003017 Msa1230040:CDS 35.0%
! AATTTTTGCCTACTGCGTTT+GGG + chr8_2:6002999-6003018 Msa1230040:CDS 35.0%
TTTGGGAAGAAGATTAAGGA+GGG + chr8_2:6003016-6003035 Msa1230040:CDS 35.0%
GAAATTCGAGAGTCATACAA+AGG + chr8_2:6003138-6003157 Msa1230040:CDS 35.0%
!! CTTGAGAAGATGGGTTATAA+AGG + chr8_2:6003178-6003197 Msa1230040:CDS 35.0%
!! TGAGAAGATGGGTTATAAAG+GGG + chr8_2:6003180-6003199 Msa1230040:CDS 35.0%
!! GGGTATTTTGAATCCTATTG+AGG + chr8_2:6003225-6003244 Msa1230040:CDS 35.0%
AAGTTGAGGGCTAAAAATTC+TGG + chr8_2:6003250-6003269 Msa1230040:CDS 35.0%
TCTTGGATTTAACGAATCGA+AGG + chr8_2:6003273-6003292 Msa1230040:CDS 35.0%
CTTGGATTTAACGAATCGAA+GGG + chr8_2:6003274-6003293 Msa1230040:CDS 35.0%
! GGAAAAGTATGTAACTGCTA+AGG + chr8_2:6003417-6003436 Msa1230040:CDS 35.0%
! TTTGTCTGATTTGAATGCTG+AGG + chr8_2:6003528-6003547 Msa1230040:CDS 35.0%
TCTATCAATCTCTTGGATGT+TGG - chr8_2:6003629-6003648 None:intergenic 35.0%
ATAGAGATTTGAGGAAAGAG+AGG + chr8_2:6003644-6003663 Msa1230040:CDS 35.0%
TAGAGATTTGAGGAAAGAGA+GGG + chr8_2:6003645-6003664 Msa1230040:CDS 35.0%
TGAAAAGATAATGAGTGTGC+TGG + chr8_2:6003747-6003766 Msa1230040:CDS 35.0%
CACCTTAGGAACATTAACAT+TGG + chr8_2:6003789-6003808 Msa1230040:CDS 35.0%
TTATCAGAATGTTCCAAGGA+TGG + chr8_2:6003914-6003933 Msa1230040:CDS 35.0%
TATCAGAATGTTCCAAGGAT+GGG + chr8_2:6003915-6003934 Msa1230040:CDS 35.0%
GTCATCACCTTATACTCAAT+TGG + chr8_2:6004029-6004048 Msa1230040:CDS 35.0%
GAGTATCTGGTATCAATACT+TGG + chr8_2:6004079-6004098 Msa1230040:CDS 35.0%
ATAGTAGCAAGAACTGAAGA+AGG - chr8_2:6004162-6004181 None:intergenic 35.0%
ATAGGAATAGTCTCAAGATG+TGG - chr8_2:6004237-6004256 None:intergenic 35.0%
ATCAAATACAGTCTTCCAAG+GGG - chr8_2:6004394-6004413 None:intergenic 35.0%
GAATCAAATACAGTCTTCCA+AGG - chr8_2:6004396-6004415 None:intergenic 35.0%
CTGTATTTGATTCTGATAGC+TGG + chr8_2:6004403-6004422 Msa1230040:CDS 35.0%
! TCAGCTTTGGTACGATAAAA+CGG - chr8_2:6004443-6004462 None:intergenic 35.0%
! TTTTATCGTACCAAAGCTGA+AGG + chr8_2:6004443-6004462 Msa1230040:CDS 35.0%
TTACAAAATGGTATTTGGGC+TGG + chr8_2:6004649-6004668 Msa1230040:CDS 35.0%
ATTCGATACAAGCTCAATTG+TGG + chr8_2:6004711-6004730 Msa1230040:CDS 35.0%
AGGAGAAGATAAGCTATATC+AGG + chr8_2:6004790-6004809 Msa1230040:CDS 35.0%
GGAGAAGATAAGCTATATCA+GGG + chr8_2:6004791-6004810 Msa1230040:CDS 35.0%
ATCTTATTGAGGCTTATGCT+CGG + chr8_2:6004928-6004947 Msa1230040:CDS 35.0%
CACAACGGTCATCAAATATA+TGG + chr8_2:6004990-6005009 Msa1230040:CDS 35.0%
GGTCATCAAATATATGGGTT+TGG + chr8_2:6004996-6005015 Msa1230040:CDS 35.0%
AGAGATGGAGAATTTCGGAA+CGG + chr8_2:6002565-6002584 Msa1230040:CDS 40.0%
! GGAACGGAGAAAGATTTTGA+AGG + chr8_2:6002581-6002600 Msa1230040:CDS 40.0%
!! ACGGAGAAAGATTTTGAAGG+TGG + chr8_2:6002584-6002603 Msa1230040:CDS 40.0%
! AGGATGATGGTGAAGAAGAT+AGG + chr8_2:6002849-6002868 Msa1230040:CDS 40.0%
AAGAAGATAGGCCTGGTTTA+GGG + chr8_2:6002861-6002880 Msa1230040:CDS 40.0%
!! CTGGTTTAGGGTTAGGTTTT+GGG + chr8_2:6002873-6002892 Msa1230040:CDS 40.0%
!!! TTTAGGGTTAGGTTTTGGGT+CGG + chr8_2:6002877-6002896 Msa1230040:CDS 40.0%
TTAGGATTTAATGCTGGTCG+TGG + chr8_2:6002920-6002939 Msa1230040:CDS 40.0%
TGCGTTTGGGAAGAAGATTA+AGG + chr8_2:6003012-6003031 Msa1230040:CDS 40.0%
GTTTGGGAAGAAGATTAAGG+AGG + chr8_2:6003015-6003034 Msa1230040:CDS 40.0%
GAGGAGAAGAAGAAGAAGAA+GGG + chr8_2:6003067-6003086 Msa1230040:CDS 40.0%
AGGAGAAGAAGAAGAAGAAG+GGG + chr8_2:6003068-6003087 Msa1230040:CDS 40.0%
GAGAGTCATACAAAGGGAAT+TGG + chr8_2:6003145-6003164 Msa1230040:CDS 40.0%
AATGAAGCTGCTTGAGAAGA+TGG + chr8_2:6003168-6003187 Msa1230040:CDS 40.0%
ATGAAGCTGCTTGAGAAGAT+GGG + chr8_2:6003169-6003188 Msa1230040:CDS 40.0%
!! GAGAAGATGGGTTATAAAGG+GGG + chr8_2:6003181-6003200 Msa1230040:CDS 40.0%
ATCCTATTGAGGCAAAGTTG+AGG + chr8_2:6003236-6003255 Msa1230040:CDS 40.0%
TCCTATTGAGGCAAAGTTGA+GGG + chr8_2:6003237-6003256 Msa1230040:CDS 40.0%
AGGGCTAAAAATTCTGGTCT+TGG + chr8_2:6003256-6003275 Msa1230040:CDS 40.0%
TTCTTCTCTGTCTGCAAAAC+GGG - chr8_2:6003316-6003335 None:intergenic 40.0%
CTTCTTCTCTGTCTGCAAAA+CGG - chr8_2:6003317-6003336 None:intergenic 40.0%
TGTAACTGCTAAGGAGTTGT+TGG + chr8_2:6003426-6003445 Msa1230040:CDS 40.0%
TAAGCAAGAAGAAGAGTCAG+AGG + chr8_2:6003453-6003472 Msa1230040:CDS 40.0%
TCTGTTAAAACTCGTACCTG+CGG - chr8_2:6003508-6003527 None:intergenic 40.0%
TATACTGAAGCTCAGGCATT+GGG - chr8_2:6003576-6003595 None:intergenic 40.0%
TTATACTGAAGCTCAGGCAT+TGG - chr8_2:6003577-6003596 None:intergenic 40.0%
TTCCCTTGCTCAATGCTTTA+CGG + chr8_2:6003813-6003832 Msa1230040:CDS 40.0%
!! CATGCACAAAAGATTTGCTG+AGG + chr8_2:6003837-6003856 Msa1230040:CDS 40.0%
TCGAAATCCATCTCATGGTT+TGG + chr8_2:6003945-6003964 Msa1230040:CDS 40.0%
GTATCAATACTTGGCATGCA+CGG + chr8_2:6004088-6004107 Msa1230040:CDS 40.0%
TATCAATACTTGGCATGCAC+GGG + chr8_2:6004089-6004108 Msa1230040:CDS 40.0%
!! AAAAACCGTAGCATTGGTTC+AGG - chr8_2:6004117-6004136 None:intergenic 40.0%
! TGAACCAATGCTACGGTTTT+TGG + chr8_2:6004116-6004135 Msa1230040:CDS 40.0%
CCAAAACTATCAAGTGCAGT+GGG + chr8_2:6004201-6004220 Msa1230040:CDS 40.0%
AGACTATTCCTATCCACACA+TGG + chr8_2:6004244-6004263 Msa1230040:CDS 40.0%
GACTATTCCTATCCACACAT+GGG + chr8_2:6004245-6004264 Msa1230040:CDS 40.0%
CATATGCAGAACCATCACTT+GGG - chr8_2:6004362-6004381 None:intergenic 40.0%
CATATGCTATATTGTCCCCT+TGG + chr8_2:6004376-6004395 Msa1230040:CDS 40.0%
! TGATCAAGATTCTGACTTGC+CGG - chr8_2:6004498-6004517 None:intergenic 40.0%
GGCTATTCCAATTCATCTGA+TGG + chr8_2:6004545-6004564 Msa1230040:CDS 40.0%
GTGTCAACCATCAGATGAAT+TGG - chr8_2:6004555-6004574 None:intergenic 40.0%
TCATCTGATGGTTGACACAA+TGG + chr8_2:6004557-6004576 Msa1230040:CDS 40.0%
ATACAAAACCGTAAGCCACT+TGG - chr8_2:6004595-6004614 None:intergenic 40.0%
! TGGTCTTGATATGATGAACC+AGG + chr8_2:6004731-6004750 Msa1230040:CDS 40.0%
CTTATCTTCTCCTTCAAACC+AGG - chr8_2:6004783-6004802 None:intergenic 40.0%
ATATCAGGGTAAGTGAGCAA+AGG + chr8_2:6004805-6004824 Msa1230040:CDS 40.0%
GAGCGTGAAAGATCTTATTG+AGG + chr8_2:6004917-6004936 Msa1230040:CDS 40.0%
TCTTATTGAGGCTTATGCTC+GGG + chr8_2:6004929-6004948 Msa1230040:CDS 40.0%
TTGTGCGTTCTACCAGATTT+AGG - chr8_2:6004975-6004994 None:intergenic 40.0%
AAATCTGGTAGAACGCACAA+CGG + chr8_2:6004975-6004994 Msa1230040:CDS 40.0%
AAAGGTCTATGCCCAAAATG+AGG + chr8_2:6005052-6005071 Msa1230040:CDS 40.0%
! AGACCATGTCTCCTCATTTT+GGG - chr8_2:6005066-6005085 None:intergenic 40.0%
! AAGACCATGTCTCCTCATTT+TGG - chr8_2:6005067-6005086 None:intergenic 40.0%
!! GAAGGTGGTCAATGGGTTAA+TGG + chr8_2:6002599-6002618 Msa1230040:CDS 45.0%
CCATCATCATCGTAGTCTTC+GGG - chr8_2:6002704-6002723 None:intergenic 45.0%
CCCATCATCATCGTAGTCTT+CGG - chr8_2:6002705-6002724 None:intergenic 45.0%
CCGAAGACTACGATGATGAT+GGG + chr8_2:6002702-6002721 Msa1230040:CDS 45.0%
GAAGAAGATAGGCCTGGTTT+AGG + chr8_2:6002860-6002879 Msa1230040:CDS 45.0%
CCAAAACCTAACCCTAAACC+AGG - chr8_2:6002875-6002894 None:intergenic 45.0%
!! CCTGGTTTAGGGTTAGGTTT+TGG + chr8_2:6002872-6002891 Msa1230040:CDS 45.0%
!! TTTTGGGTCGGCTAGTATCT+CGG + chr8_2:6002889-6002908 Msa1230040:CDS 45.0%
ATCTTCTTCCCAAACGCAGT+AGG - chr8_2:6003010-6003029 None:intergenic 45.0%
GGAGGAGAAGAAGAAGAAGA+AGG + chr8_2:6003066-6003085 Msa1230040:CDS 45.0%
!! AAGATGGGTTATAAAGGGGG+TGG + chr8_2:6003184-6003203 Msa1230040:CDS 45.0%
GTTATAAAGGGGGTGGTCTT+GGG + chr8_2:6003191-6003210 Msa1230040:CDS 45.0%
GACAGAGAAGAAGACTGAAC+CGG + chr8_2:6003324-6003343 Msa1230040:CDS 45.0%
ACAGAGAAGAAGACTGAACC+GGG + chr8_2:6003325-6003344 Msa1230040:CDS 45.0%
GAACGTGTTCTTCCAACTGT+AGG - chr8_2:6003361-6003380 None:intergenic 45.0%
GAATGCTGAGGAGAAAGCAA+AGG + chr8_2:6003540-6003559 Msa1230040:CDS 45.0%
GCTACGTTATACTGAAGCTC+AGG - chr8_2:6003583-6003602 None:intergenic 45.0%
AAAGGAGAAGTTGGAAGCTG+AGG + chr8_2:6003693-6003712 Msa1230040:CDS 45.0%
GTTGGAGAAGAGAACACCTT+AGG + chr8_2:6003775-6003794 Msa1230040:CDS 45.0%
!! GTCCGTAAAGCATTGAGCAA+GGG - chr8_2:6003818-6003837 None:intergenic 45.0%
!! TGTCCGTAAAGCATTGAGCA+AGG - chr8_2:6003819-6003838 None:intergenic 45.0%
! CCATGAGATGGATTTCGAAG+AGG - chr8_2:6003943-6003962 None:intergenic 45.0%
CCTCTTCGAAATCCATCTCA+TGG + chr8_2:6003940-6003959 Msa1230040:CDS 45.0%
! TCCATCTCATGGTTTGGAGT+TGG + chr8_2:6003951-6003970 Msa1230040:CDS 45.0%
ACCAACTCCAAACCATGAGA+TGG - chr8_2:6003955-6003974 None:intergenic 45.0%
!! GTTTGGAGTTGGTATCACAG+TGG + chr8_2:6003962-6003981 Msa1230040:CDS 45.0%
ATACCAGATACTCTGACAGC+TGG - chr8_2:6004072-6004091 None:intergenic 45.0%
GACTCCAAAAACCGTAGCAT+TGG - chr8_2:6004123-6004142 None:intergenic 45.0%
!!! TGCTACGGTTTTTGGAGTCT+TGG + chr8_2:6004124-6004143 Msa1230040:CDS 45.0%
!!! GCTACGGTTTTTGGAGTCTT+GGG + chr8_2:6004125-6004144 Msa1230040:CDS 45.0%
GCCAAAACTATCAAGTGCAG+TGG + chr8_2:6004200-6004219 Msa1230040:CDS 45.0%
!! CCCACTGCACTTGATAGTTT+TGG - chr8_2:6004204-6004223 None:intergenic 45.0%
TATCAAGTGCAGTGGGTACT+TGG + chr8_2:6004208-6004227 Msa1230040:CDS 45.0%
ATCAAGTGCAGTGGGTACTT+GGG + chr8_2:6004209-6004228 Msa1230040:CDS 45.0%
! TGAGTACTGTTCTTGGTGCA+TGG + chr8_2:6004334-6004353 Msa1230040:CDS 45.0%
GCATATGCAGAACCATCACT+TGG - chr8_2:6004363-6004382 None:intergenic 45.0%
AGATGACTTCCAAGTGAACC+CGG + chr8_2:6004476-6004495 Msa1230040:CDS 45.0%
! GATCAAGATTCTGACTTGCC+GGG - chr8_2:6004497-6004516 None:intergenic 45.0%
TGGAGATCTTCTTTGCCAAG+TGG + chr8_2:6004577-6004596 Msa1230040:CDS 45.0%
CTTCTTTGCCAAGTGGCTTA+CGG + chr8_2:6004584-6004603 Msa1230040:CDS 45.0%
!! GGCTTACGGTTTTGTATCGT+TGG + chr8_2:6004598-6004617 Msa1230040:CDS 45.0%
ATGATGAACCAGGCTGTTGA+AGG + chr8_2:6004741-6004760 Msa1230040:CDS 45.0%
ATGCCCAAAATGAGGAGACA+TGG + chr8_2:6005060-6005079 Msa1230040:CDS 45.0%
CCCGAAGACTACGATGATGA+TGG + chr8_2:6002701-6002720 Msa1230040:CDS 50.0%
GATGGTGAAGAAGATAGGCC+TGG + chr8_2:6002854-6002873 Msa1230040:CDS 50.0%
! GATAGGCCTGGTTTAGGGTT+AGG + chr8_2:6002866-6002885 Msa1230040:CDS 50.0%
TTTGGGTCGGCTAGTATCTC+GGG + chr8_2:6002890-6002909 Msa1230040:CDS 50.0%
! AGTATCTCGGGGTCTGGTTT+AGG + chr8_2:6002902-6002921 Msa1230040:CDS 50.0%
AATGCTGGTCGTGGTGTGAA+TGG + chr8_2:6002929-6002948 Msa1230040:CDS 50.0%
TTAAGGAGGGAGCCATGAAG+AGG + chr8_2:6003029-6003048 Msa1230040:CDS 50.0%
TAAGGAGGGAGCCATGAAGA+GGG + chr8_2:6003030-6003049 Msa1230040:CDS 50.0%
ACTCTCCTTCTCCCTCTTCA+TGG - chr8_2:6003044-6003063 None:intergenic 50.0%
GAAGAGGGAGAAGGAGAGTT+TGG + chr8_2:6003045-6003064 Msa1230040:CDS 50.0%
GAAGAAGAAGGGGAAGAAGC+CGG + chr8_2:6003078-6003097 Msa1230040:CDS 50.0%
AAGAAGAAGGGGAAGAAGCC+GGG + chr8_2:6003079-6003098 Msa1230040:CDS 50.0%
!! AAGAAGCCGGGCATTGGTTT+AGG + chr8_2:6003091-6003110 Msa1230040:CDS 50.0%
TCCTGACCTAAACCAATGCC+CGG - chr8_2:6003100-6003119 None:intergenic 50.0%
!! GGCATTGGTTTAGGTCAGGA+GGG + chr8_2:6003100-6003119 Msa1230040:CDS 50.0%
GGTTATAAAGGGGGTGGTCT+TGG + chr8_2:6003190-6003209 Msa1230040:CDS 50.0%
! TGGTCTTGGGAAGAATGAGC+AGG + chr8_2:6003204-6003223 Msa1230040:CDS 50.0%
GGTCTTGGGAAGAATGAGCA+GGG + chr8_2:6003205-6003224 Msa1230040:CDS 50.0%
GCCCTCAACTTTGCCTCAAT+AGG - chr8_2:6003241-6003260 None:intergenic 50.0%
TCTGTCTGCAAAACGGGCAA+CGG - chr8_2:6003310-6003329 None:intergenic 50.0%
CAGAGAAGAAGACTGAACCG+GGG + chr8_2:6003326-6003345 Msa1230040:CDS 50.0%
AGAGAAGAAGACTGAACCGG+GGG + chr8_2:6003327-6003346 Msa1230040:CDS 50.0%
CAGTTGGAAGAACACGTTCG+TGG + chr8_2:6003362-6003381 Msa1230040:CDS 50.0%
ATGAGTGTGCTGGACAGAGT+TGG + chr8_2:6003757-6003776 Msa1230040:CDS 50.0%
TTCGAAGAGGATCCCATCCT+TGG - chr8_2:6003930-6003949 None:intergenic 50.0%
!! GGAGTTGGTATCACAGTGGA+AGG + chr8_2:6003966-6003985 Msa1230040:CDS 50.0%
!! CAGTGGAAGGCATTGCTTCA+AGG + chr8_2:6003979-6003998 Msa1230040:CDS 50.0%
! TTGCCAGCTGTCAGAGTATC+TGG + chr8_2:6004066-6004085 Msa1230040:CDS 50.0%
GGGATCCTGAACCAATGCTA+CGG + chr8_2:6004109-6004128 Msa1230040:CDS 50.0%
TGCAAGACAGCCTTCAGCTT+TGG - chr8_2:6004456-6004475 None:intergenic 50.0%
CTGGGTTATGAACTGGGCTT+CGG + chr8_2:6004524-6004543 Msa1230040:CDS 50.0%
GAACCAGGCTGTTGAAGGTA+TGG + chr8_2:6004746-6004765 Msa1230040:CDS 50.0%
! GGTTGTGCAACCTGGTTTGA+AGG + chr8_2:6004770-6004789 Msa1230040:CDS 50.0%
!! AGCAGCTTGTTGTTGAGCAG+AGG - chr8_2:6004859-6004878 None:intergenic 50.0%
ACAACAAGCTGCTGCAAGCA+TGG + chr8_2:6004866-6004885 Msa1230040:CDS 50.0%
GAGGCTTATGCTCGGGATAA+TGG + chr8_2:6004936-6004955 Msa1230040:CDS 50.0%
TTGGGTCGGCTAGTATCTCG+GGG + chr8_2:6002891-6002910 Msa1230040:CDS 55.0%
GAGGGAGAAGGAGAGTTTGG+AGG + chr8_2:6003048-6003067 Msa1230040:CDS 55.0%
!! GGGCATTGGTTTAGGTCAGG+AGG + chr8_2:6003099-6003118 Msa1230040:CDS 55.0%
TTTAGGTCAGGAGGGAGCTG+TGG + chr8_2:6003108-6003127 Msa1230040:CDS 55.0%
AGGAGGGAGCTGTGGATGTT+GGG + chr8_2:6003116-6003135 Msa1230040:CDS 55.0%
CAAAACGGGCAACGGCATAG+TGG - chr8_2:6003302-6003321 None:intergenic 55.0%
TTATGATATGAGGGGGCCGC+AGG + chr8_2:6003489-6003508 Msa1230040:CDS 55.0%
GGATGCACCCATGTGTGGAT+AGG - chr8_2:6004255-6004274 None:intergenic 55.0%
TCCACACATGGGTGCATCCA+TGG + chr8_2:6004256-6004275 Msa1230040:CDS 55.0%
ATCCATGGCTACCCCTGCTA+GGG + chr8_2:6004271-6004290 Msa1230040:CDS 55.0%
! TCTGACTTGCCGGGTTCACT+TGG - chr8_2:6004488-6004507 None:intergenic 55.0%
CCTCCATACCTTCAACAGCC+TGG - chr8_2:6004752-6004771 None:intergenic 55.0%
! CCAGGCTGTTGAAGGTATGG+AGG + chr8_2:6004749-6004768 Msa1230040:CDS 55.0%
GGTATGGAGGTTGTGCAACC+TGG + chr8_2:6004762-6004781 Msa1230040:CDS 55.0%
TCGGCTAGTATCTCGGGGTC+TGG + chr8_2:6002896-6002915 Msa1230040:CDS 60.0%
GGGAGCCATGAAGAGGGAGA+AGG + chr8_2:6003036-6003055 Msa1230040:CDS 60.0%
AAGGGGAAGAAGCCGGGCAT+TGG + chr8_2:6003085-6003104 Msa1230040:CDS 60.0%
!! GCCGGGCATTGGTTTAGGTC+AGG + chr8_2:6003096-6003115 Msa1230040:CDS 60.0%
CAGGAGGGAGCTGTGGATGT+TGG + chr8_2:6003115-6003134 Msa1230040:CDS 60.0%
ACTGTAGGCTGCACAACCCC+CGG - chr8_2:6003346-6003365 None:intergenic 60.0%
GGGGTTGTGCAGCCTACAGT+TGG + chr8_2:6003346-6003365 Msa1230040:CDS 60.0%
GCCATGGATGCACCCATGTG+TGG - chr8_2:6004260-6004279 None:intergenic 60.0%
CATCCATGGCTACCCCTGCT+AGG + chr8_2:6004270-6004289 Msa1230040:CDS 60.0%
TCCAGCTTGTGCCCTAGCAG+GGG - chr8_2:6004285-6004304 None:intergenic 60.0%
CTCCAGCTTGTGCCCTAGCA+GGG - chr8_2:6004286-6004305 None:intergenic 60.0%
GGTGCATGGCACCCAAGTGA+TGG + chr8_2:6004348-6004367 Msa1230040:CDS 60.0%
CAAGCTGCTGCAAGCATGGC+TGG + chr8_2:6004870-6004889 Msa1230040:CDS 60.0%
TGCCCTAGCAGGGGTAGCCA+TGG - chr8_2:6004276-6004295 None:intergenic 65.0%
ACCCCTGCTAGGGCACAAGC+TGG + chr8_2:6004281-6004300 Msa1230040:CDS 65.0%
CCTCCAGCTTGTGCCCTAGC+AGG - chr8_2:6004287-6004306 None:intergenic 65.0%
CCTGCTAGGGCACAAGCTGG+AGG + chr8_2:6004284-6004303 Msa1230040:CDS 65.0%
GCTGCTGCAAGCATGGCTGG+TGG + chr8_2:6004873-6004892 Msa1230040:CDS 65.0%
! AGCATGGCTGGTGGCGCAGA+TGG + chr8_2:6004882-6004901 Msa1230040:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 6002545 6005145 6002545 ID=Msa1230040;Name=Msa1230040
chr8_2 mRNA 6002545 6005145 6002545 ID=Msa1230040-mRNA-1;Parent=Msa1230040;Name=Msa1230040-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|-1|0|1|-1|1|1|0|866
chr8_2 exon 6002545 6005145 6002545 ID=Msa1230040-mRNA-1:exon:2136;Parent=Msa1230040-mRNA-1
chr8_2 CDS 6002545 6005145 6002545 ID=Msa1230040-mRNA-1:cds;Parent=Msa1230040-mRNA-1
Gene Sequence

>Msa1230040

ATGGATATAGATGAGGATCAAGAGATGGAGAATTTCGGAACGGAGAAAGATTTTGAAGGTGGTCAATGGGTTAATGGTGAATTCTACTTTCGAAAGCGCCGAGAGAAAGCTCAAACTCAAACCAAAGACGATGTTATATATGGAGTTTTTGGTGATCCCGAAGACTACGATGATGATGGGTATTCTAGTAAAAAGCGAAGAAAAGATTTTGGAAGGAAACAGGATCTCACTAAACCTGTGAATTTTGTTTCAACTGGAAATTTTATGCCCGATAAGGATAATGTTGATGAGAATTTGAAAGAACAGGATGATGGTGAAGAAGATAGGCCTGGTTTAGGGTTAGGTTTTGGGTCGGCTAGTATCTCGGGGTCTGGTTTAGGATTTAATGCTGGTCGTGGTGTGAATGGTTCAGATAGAAATGAGAAATCCGATGAGAATGATAATCGGGATGATAAATTTTTGCCTACTGCGTTTGGGAAGAAGATTAAGGAGGGAGCCATGAAGAGGGAGAAGGAGAGTTTGGAGGAGAAGAAGAAGAAGAAGGGGAAGAAGCCGGGCATTGGTTTAGGTCAGGAGGGAGCTGTGGATGTTGGGAAATTCGAGAGTCATACAAAGGGAATTGGAATGAAGCTGCTTGAGAAGATGGGTTATAAAGGGGGTGGTCTTGGGAAGAATGAGCAGGGTATTTTGAATCCTATTGAGGCAAAGTTGAGGGCTAAAAATTCTGGTCTTGGATTTAACGAATCGAAGGGAACCACTATGCCGTTGCCCGTTTTGCAGACAGAGAAGAAGACTGAACCGGGGGTTGTGCAGCCTACAGTTGGAAGAACACGTTCGTGGTTAAAGCCGCCGAAGAAAAAAAATAAAGAAGAGGAAAAGTATGTAACTGCTAAGGAGTTGTTGGCTAGTAAGCAAGAAGAAGAGTCAGAGGTTGTTCAGAAGATTTATGATATGAGGGGGCCGCAGGTACGAGTTTTAACAGATTTGTCTGATTTGAATGCTGAGGAGAAAGCAAAGGAAAATGATGTCCCAATGCCTGAGCTTCAGTATAACGTAGCACTAATAGTTCGGATAGCTGAATCCAACATCCAAGAGATTGATAGAGATTTGAGGAAAGAGAGGGATATAGCACTTAGCTTGAAAAAAGAAAAGGAGAAGTTGGAAGCTGAGGCAGCGTTTCAAAAGAAGCAGCTCGATAATTATGAAAAGATAATGAGTGTGCTGGACAGAGTTGGAGAAGAGAACACCTTAGGAACATTAACATTGGATTCCCTTGCTCAATGCTTTACGGACATGCACAAAAGATTTGCTGAGGATTATAAGCTGTGTAACTTGTCATGCATTGCTTGCTCATATGCTCTGCCTTTGTTTATCAGAATGTTCCAAGGATGGGATCCTCTTCGAAATCCATCTCATGGTTTGGAGTTGGTATCACAGTGGAAGGCATTGCTTCAAGGAGATGATTGTTTTGATATATGGGACATGTCATCACCTTATACTCAATTGGTTTCAGAGGTTGTATTGCCAGCTGTCAGAGTATCTGGTATCAATACTTGGCATGCACGGGATCCTGAACCAATGCTACGGTTTTTGGAGTCTTGGGAAAAATTGCTTCCTTCTTCAGTTCTTGCTACTATATTAGACAACATAGTCATGCCAAAACTATCAAGTGCAGTGGGTACTTGGGAACCACATCTTGAGACTATTCCTATCCACACATGGGTGCATCCATGGCTACCCCTGCTAGGGCACAAGCTGGAGGTTATCTACCAGACAATTCGTTTCAAATTGAGTACTGTTCTTGGTGCATGGCACCCAAGTGATGGTTCTGCATATGCTATATTGTCCCCTTGGAAGACTGTATTTGATTCTGATAGCTGGCAACAACTTATGCACCGTTTTATCGTACCAAAGCTGAAGGCTGTCTTGCAAGATGACTTCCAAGTGAACCCGGCAAGTCAGAATCTTGATCAATTTTACTGGGTTATGAACTGGGCTTCGGCTATTCCAATTCATCTGATGGTTGACACAATGGAGATCTTCTTTGCCAAGTGGCTTACGGTTTTGTATCGTTGGTTGTGCTCAAATCCTAACTTTGATGAAGTTACAAAATGGTATTTGGGCTGGAAAGAACTTATTCCAAAAGAACTTTTGGCAAATGAAAGTATTCGATACAAGCTCAATTGTGGTCTTGATATGATGAACCAGGCTGTTGAAGGTATGGAGGTTGTGCAACCTGGTTTGAAGGAGAAGATAAGCTATATCAGGGTAAGTGAGCAAAGGAAATTTGAGACTCAGCAGAAAGCAGCAGCCTCTGCTCAACAACAAGCTGCTGCAAGCATGGCTGGTGGCGCAGATGGTGTGAATGAAATGAGCGTGAAAGATCTTATTGAGGCTTATGCTCGGGATAATGGTTTACTGTTCAAACCTAAATCTGGTAGAACGCACAACGGTCATCAAATATATGGGTTTGGCAATGTCAGCATAATAATCGACTCTCTAAATCAAAAGGTCTATGCCCAAAATGAGGAGACATGGTCTTTAGAAACTCTTGAACGATTGCTAGAGTTACATAATAAATCCCTTAGTAAAAGACGCTGA
Protein sequence

>Msa1230040

MDIDEDQEMENFGTEKDFEGGQWVNGEFYFRKRREKAQTQTKDDVIYGVFGDPEDYDDDGYSSKKRRKDFGRKQDLTKPVNFVSTGNFMPDKDNVDENLKEQDDGEEDRPGLGLGFGSASISGSGLGFNAGRGVNGSDRNEKSDENDNRDDKFLPTAFGKKIKEGAMKREKESLEEKKKKKGKKPGIGLGQEGAVDVGKFESHTKGIGMKLLEKMGYKGGGLGKNEQGILNPIEAKLRAKNSGLGFNESKGTTMPLPVLQTEKKTEPGVVQPTVGRTRSWLKPPKKKNKEEEKYVTAKELLASKQEEESEVVQKIYDMRGPQVRVLTDLSDLNAEEKAKENDVPMPELQYNVALIVRIAESNIQEIDRDLRKERDIALSLKKEKEKLEAEAAFQKKQLDNYEKIMSVLDRVGEENTLGTLTLDSLAQCFTDMHKRFAEDYKLCNLSCIACSYALPLFIRMFQGWDPLRNPSHGLELVSQWKALLQGDDCFDIWDMSSPYTQLVSEVVLPAVRVSGINTWHARDPEPMLRFLESWEKLLPSSVLATILDNIVMPKLSSAVGTWEPHLETIPIHTWVHPWLPLLGHKLEVIYQTIRFKLSTVLGAWHPSDGSAYAILSPWKTVFDSDSWQQLMHRFIVPKLKAVLQDDFQVNPASQNLDQFYWVMNWASAIPIHLMVDTMEIFFAKWLTVLYRWLCSNPNFDEVTKWYLGWKELIPKELLANESIRYKLNCGLDMMNQAVEGMEVVQPGLKEKISYIRVSEQRKFETQQKAAASAQQQAAASMAGGADGVNEMSVKDLIEAYARDNGLLFKPKSGRTHNGHQIYGFGNVSIIIDSLNQKVYAQNEETWSLETLERLLELHNKSLSKRR*