AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1315970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1315970 MtrunA17_Chr8g0347771 96.512 86 3 0 1 86 158 243 1.10e-53 166
Msa1315970 MtrunA17_Chr8g0345761 93.023 86 6 0 1 86 211 296 1.95e-51 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1315970 AT4G05400.1 69.412 85 26 0 2 86 166 250 5.39e-35 119
Msa1315970 AT4G05400.2 69.412 85 26 0 2 86 166 250 5.39e-35 119
Msa1315970 AT4G05400.3 69.412 85 26 0 2 86 166 250 5.39e-35 119
Msa1315970 AT4G21140.1 64.368 87 30 1 1 86 157 243 2.65e-31 110

Find 49 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCGTTAGCTATTGACTTC+TGG 0.182334 8_4:-15627301 Msa1315970:three_prime_UTR
GTTTCCGGAGAATAGGTTTA+TGG 0.260771 8_4:-15629322 Msa1315970:CDS
TGTAAACTTCTAAAGCTTCT+AGG 0.266135 8_4:+15627108 None:intergenic
TGTAAACTTCTAAAGCTTCT+AGG 0.266135 8_4:+15627163 None:intergenic
GACTAGCCTTTGTGGAGTTC+TGG 0.270021 8_4:-15627442 Msa1315970:three_prime_UTR
TGCGTTAGCTATTGACTTCT+GGG 0.302245 8_4:-15627300 Msa1315970:three_prime_UTR
GAATCTATTGAGGATGAAAA+AGG 0.364273 8_4:-15629406 Msa1315970:CDS
GGGTTTGAAGGAAGCTGCTT+TGG 0.367116 8_4:-15629361 Msa1315970:CDS
CAAGGAAGATGGGATGAAAT+TGG 0.370390 8_4:-15629487 None:intergenic
AATTCAGTGAAGTCATGTTT+AGG 0.378457 8_4:+15627201 None:intergenic
TCAGTGGGAAGGAGAAGATT+AGG 0.382186 8_4:-15629239 Msa1315970:CDS
TTACGCCGTTTCCGGAGAAT+AGG 0.397560 8_4:-15629329 Msa1315970:CDS
GAGGCTATTGAAGCTTTGCC+TGG 0.402944 8_4:-15629384 Msa1315970:CDS
TTACATTATTATGATTATAG+TGG 0.413381 8_4:-15627090 Msa1315970:three_prime_UTR
AATTGGCCAAGGAGTATAGT+AGG 0.424942 8_4:-15629470 Msa1315970:CDS
ACGCTTACGCCGCCTATTGA+TGG 0.425057 8_4:-15629297 Msa1315970:CDS
AGTTTACATTATGATTATAG+TGG 0.437138 8_4:-15627148 Msa1315970:three_prime_UTR
TGGGTATATTGAGAAGATTA+AGG 0.447659 8_4:-15629277 Msa1315970:CDS
CTGGTCAGGACTAGCCTTTG+TGG 0.448822 8_4:-15627450 Msa1315970:intron
TCCGGAAACGGCGTAAAATC+CGG 0.450268 8_4:+15629336 None:intergenic
GATTAGGTAGATTGCGTCAC+AGG 0.459115 8_4:-15629223 Msa1315970:intron
AGATTAAGGAGGCTGCTGAT+AGG 0.463125 8_4:-15629263 Msa1315970:CDS
TTCTCAATATACCCATCAAT+AGG 0.469115 8_4:+15629285 None:intergenic
GAAGGAAGCTGCTTTGGTGC+CGG 0.473891 8_4:-15629355 Msa1315970:CDS
GGCTATTGAAGCTTTGCCTG+GGG 0.503885 8_4:-15629382 Msa1315970:CDS
CATTCAGATATTAGTAAGCC+AGG 0.514997 8_4:-15627330 Msa1315970:three_prime_UTR
AGTAACTTGTAAGAAAATGA+AGG 0.516600 8_4:+15627412 None:intergenic
ATAAACCTATTCTCCGGAAA+CGG 0.516651 8_4:+15629324 None:intergenic
CGCTTACGCCGCCTATTGAT+GGG 0.516719 8_4:-15629296 Msa1315970:CDS
TAGCTAACGCAAGAGAAGCC+TGG 0.537970 8_4:+15627312 None:intergenic
AGGCTATTGAAGCTTTGCCT+GGG 0.539955 8_4:-15629383 Msa1315970:CDS
CAAAACCCTACTATACTCCT+TGG 0.553845 8_4:+15629464 None:intergenic
CGGCGGAGACGAATCTATTG+AGG 0.575732 8_4:-15629416 Msa1315970:CDS
ATTGGCCAAGGAGTATAGTA+GGG 0.576553 8_4:-15629469 Msa1315970:CDS
GTTGCCATAAACCTATTCTC+CGG 0.584330 8_4:+15629318 None:intergenic
GAAGCATAGGGCGAGACAAG+CGG 0.597439 8_4:-15629436 Msa1315970:CDS
GCTATTGAAGCTTTGCCTGG+GGG 0.597757 8_4:-15629381 Msa1315970:CDS
TGCTGATAGGATCAGTGGGA+AGG 0.607298 8_4:-15629250 Msa1315970:CDS
GAGGCTGCTGATAGGATCAG+TGG 0.610487 8_4:-15629255 Msa1315970:CDS
AGATGGGATGAAATTGGCCA+AGG 0.616560 8_4:-15629481 None:intergenic
AGGCTGCTGATAGGATCAGT+GGG 0.618985 8_4:-15629254 Msa1315970:CDS
GTCGTTGCGTAGCAGTACAT+AGG 0.621497 8_4:+15627352 None:intergenic
ATTTCTGCAAACTAGTCAGT+GGG 0.630877 8_4:-15627276 Msa1315970:three_prime_UTR
TATTTCTGCAAACTAGTCAG+TGG 0.635693 8_4:-15627277 Msa1315970:three_prime_UTR
GCATAGGGCGAGACAAGCGG+CGG 0.658080 8_4:-15629433 Msa1315970:CDS
TCAATATACCCATCAATAGG+CGG 0.683015 8_4:+15629288 None:intergenic
GTATATTGAGAAGATTAAGG+AGG 0.685736 8_4:-15629274 Msa1315970:CDS
GTAACTCCAGAACTCCACAA+AGG 0.743115 8_4:+15627436 None:intergenic
TGTTACGCAAAACTCCACTG+CGG 0.859160 8_4:+15627375 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATATATTTTTTTTTTT+TGG - chr8_4:15627978-15627997 Msa1315970:intron 0.0%
!!! TAATATATATTTTTTTTTTT+TGG - chr8_4:15627978-15627997 Msa1315970:intron 0.0%
!! TTTAATTGATGAAATTTCAT+TGG - chr8_4:15627458-15627477 Msa1315970:intron 15.0%
!!! TTTTGAAATGAGAAATTTTA+GGG + chr8_4:15628314-15628333 None:intergenic 15.0%
!!! TTTTTGAAATGAGAAATTTT+AGG + chr8_4:15628315-15628334 None:intergenic 15.0%
!! TAAAATACTATAAAAAGTAG+AGG + chr8_4:15629064-15629083 None:intergenic 15.0%
!! TTACATTATTATGATTATAG+TGG - chr8_4:15629474-15629493 Msa1315970:CDS 15.0%
!! TTTAATTGATGAAATTTCAT+TGG - chr8_4:15627458-15627477 Msa1315970:intron 15.0%
!!! TTTTGAAATGAGAAATTTTA+GGG + chr8_4:15628314-15628333 None:intergenic 15.0%
!!! TTTTTGAAATGAGAAATTTT+AGG + chr8_4:15628315-15628334 None:intergenic 15.0%
!! TAAAATACTATAAAAAGTAG+AGG + chr8_4:15629064-15629083 None:intergenic 15.0%
!! TATTGAATCGAAATTGAAAT+GGG - chr8_4:15627383-15627402 Msa1315970:three_prime_UTR 20.0%
!! ATTTAATCTTGCAAATAAGA+TGG - chr8_4:15627425-15627444 Msa1315970:three_prime_UTR 20.0%
!!! TATTGCATGAATTTATCTTT+TGG + chr8_4:15628241-15628260 None:intergenic 20.0%
!!! TTTGAAATGAGAAATTTTAG+GGG + chr8_4:15628313-15628332 None:intergenic 20.0%
!! TTCAAATTTCATCAATTTAG+TGG + chr8_4:15628348-15628367 None:intergenic 20.0%
!! AGTTTACATTATGATTATAG+TGG - chr8_4:15629416-15629435 Msa1315970:CDS 20.0%
!! TATTGAATCGAAATTGAAAT+GGG - chr8_4:15627383-15627402 Msa1315970:three_prime_UTR 20.0%
!! ATTTAATCTTGCAAATAAGA+TGG - chr8_4:15627425-15627444 Msa1315970:three_prime_UTR 20.0%
!!! TATTGCATGAATTTATCTTT+TGG + chr8_4:15628241-15628260 None:intergenic 20.0%
!!! TTTGAAATGAGAAATTTTAG+GGG + chr8_4:15628313-15628332 None:intergenic 20.0%
!! TTCAAATTTCATCAATTTAG+TGG + chr8_4:15628348-15628367 None:intergenic 20.0%
!! AGTTTACATTATGATTATAG+TGG - chr8_4:15629416-15629435 Msa1315970:CDS 20.0%
! GTATTGAATCGAAATTGAAA+TGG - chr8_4:15627382-15627401 Msa1315970:three_prime_UTR 25.0%
! TGAATAGTTCAGAAACAATT+TGG + chr8_4:15627722-15627741 None:intergenic 25.0%
! AGAAAAATTGCTTAACATTC+TGG - chr8_4:15627806-15627825 Msa1315970:intron 25.0%
! GAAAAATTGCTTAACATTCT+GGG - chr8_4:15627807-15627826 Msa1315970:intron 25.0%
!!! ATAATCTTTTTTCCAGTTGA+AGG + chr8_4:15627954-15627973 None:intergenic 25.0%
!! CATAAACTAACGATCTTTTT+AGG + chr8_4:15628092-15628111 None:intergenic 25.0%
! AATTCATGCAATATTGTACA+AGG - chr8_4:15628248-15628267 Msa1315970:intron 25.0%
!! TTGAAATGAGAAATTTTAGG+GGG + chr8_4:15628312-15628331 None:intergenic 25.0%
! AAATTTGAAAATACAGCCTA+AGG - chr8_4:15628359-15628378 Msa1315970:intron 25.0%
!! TATTTTCAATTCTTAGACAG+TGG - chr8_4:15628420-15628439 Msa1315970:intron 25.0%
!! ATTTTCAATTCTTAGACAGT+GGG - chr8_4:15628421-15628440 Msa1315970:intron 25.0%
! AGAAAAATTGCTTAACATTC+TGG - chr8_4:15627806-15627825 Msa1315970:intron 25.0%
! GAAAAATTGCTTAACATTCT+GGG - chr8_4:15627807-15627826 Msa1315970:intron 25.0%
! AAAAATTGCTTAACATTCTG+GGG - chr8_4:15627808-15627827 Msa1315970:intron 25.0%
!!! ATAATCTTTTTTCCAGTTGA+AGG + chr8_4:15627954-15627973 None:intergenic 25.0%
!!! GAAGTGAGAAATTTTTTTGA+TGG - chr8_4:15628810-15628829 Msa1315970:intron 25.0%
! CTGAACTAGAAAAATTAAGT+TGG - chr8_4:15628883-15628902 Msa1315970:intron 25.0%
!!! TTAAGTTGGTTTTAACAGTT+CGG - chr8_4:15628897-15628916 Msa1315970:intron 25.0%
!! AGTAACTTGTAAGAAAATGA+AGG + chr8_4:15629155-15629174 None:intergenic 25.0%
! GTATTGAATCGAAATTGAAA+TGG - chr8_4:15627382-15627401 Msa1315970:three_prime_UTR 25.0%
! TGAATAGTTCAGAAACAATT+TGG + chr8_4:15627722-15627741 None:intergenic 25.0%
! AGAAAAATTGCTTAACATTC+TGG - chr8_4:15627806-15627825 Msa1315970:intron 25.0%
! GAAAAATTGCTTAACATTCT+GGG - chr8_4:15627807-15627826 Msa1315970:intron 25.0%
!!! ATAATCTTTTTTCCAGTTGA+AGG + chr8_4:15627954-15627973 None:intergenic 25.0%
!! CATAAACTAACGATCTTTTT+AGG + chr8_4:15628092-15628111 None:intergenic 25.0%
! AATTCATGCAATATTGTACA+AGG - chr8_4:15628248-15628267 Msa1315970:intron 25.0%
!! TTGAAATGAGAAATTTTAGG+GGG + chr8_4:15628312-15628331 None:intergenic 25.0%
! AAATTTGAAAATACAGCCTA+AGG - chr8_4:15628359-15628378 Msa1315970:intron 25.0%
!! TATTTTCAATTCTTAGACAG+TGG - chr8_4:15628420-15628439 Msa1315970:intron 25.0%
!! ATTTTCAATTCTTAGACAGT+GGG - chr8_4:15628421-15628440 Msa1315970:intron 25.0%
! AGAAAAATTGCTTAACATTC+TGG - chr8_4:15627806-15627825 Msa1315970:intron 25.0%
! GAAAAATTGCTTAACATTCT+GGG - chr8_4:15627807-15627826 Msa1315970:intron 25.0%
! AAAAATTGCTTAACATTCTG+GGG - chr8_4:15627808-15627827 Msa1315970:intron 25.0%
!!! ATAATCTTTTTTCCAGTTGA+AGG + chr8_4:15627954-15627973 None:intergenic 25.0%
!!! GAAGTGAGAAATTTTTTTGA+TGG - chr8_4:15628810-15628829 Msa1315970:intron 25.0%
! CTGAACTAGAAAAATTAAGT+TGG - chr8_4:15628883-15628902 Msa1315970:intron 25.0%
!!! TTAAGTTGGTTTTAACAGTT+CGG - chr8_4:15628897-15628916 Msa1315970:intron 25.0%
!! AGTAACTTGTAAGAAAATGA+AGG + chr8_4:15629155-15629174 None:intergenic 25.0%
GAATCTATTGAGGATGAAAA+AGG - chr8_4:15627158-15627177 Msa1315970:three_prime_UTR 30.0%
TTCTCAATATACCCATCAAT+AGG + chr8_4:15627282-15627301 None:intergenic 30.0%
TGGGTATATTGAGAAGATTA+AGG - chr8_4:15627287-15627306 Msa1315970:three_prime_UTR 30.0%
GTATATTGAGAAGATTAAGG+AGG - chr8_4:15627290-15627309 Msa1315970:three_prime_UTR 30.0%
!! GTTGAACTGAACTGATTTTA+TGG - chr8_4:15627645-15627664 Msa1315970:intron 30.0%
!! ACTGAACTGATTTTATGGTT+CGG - chr8_4:15627650-15627669 Msa1315970:intron 30.0%
! TTTTCAAACATCACTCTGTT+CGG + chr8_4:15627699-15627718 None:intergenic 30.0%
!!! TTTTGGTAGTGTAGATTATC+AGG - chr8_4:15627995-15628014 Msa1315970:intron 30.0%
TACTGTTATGAAACCTTACT+AGG - chr8_4:15628191-15628210 Msa1315970:intron 30.0%
! AGCTTAAAGCATTTCAATGA+TGG - chr8_4:15628272-15628291 Msa1315970:intron 30.0%
! AAAGCATTTCAATGATGGTT+TGG - chr8_4:15628277-15628296 Msa1315970:intron 30.0%
TACTATGATTTCTCATCCTT+AGG + chr8_4:15628378-15628397 None:intergenic 30.0%
AAAATTTCGTGATTGGAATC+TGG + chr8_4:15628787-15628806 None:intergenic 30.0%
CTTCATCAAAATTTCGTGAT+TGG + chr8_4:15628794-15628813 None:intergenic 30.0%
!!! GTGAGAAATTTTTTTGATGG+AGG - chr8_4:15628813-15628832 Msa1315970:intron 30.0%
!! GGTTTTTATGAGTTTAGTTC+AGG - chr8_4:15628938-15628957 Msa1315970:intron 30.0%
TTGAACCAACATTAACAGTT+TGG - chr8_4:15628995-15629014 Msa1315970:intron 30.0%
! GAATGTAGTCAAATTGATTC+TGG - chr8_4:15629095-15629114 Msa1315970:intron 30.0%
AATTCAGTGAAGTCATGTTT+AGG + chr8_4:15629328-15629347 None:intergenic 30.0%
!! TGTAAACTTCTAAAGCTTCT+AGG + chr8_4:15629404-15629423 None:intergenic 30.0%
!! TGTAAACTTCTAAAGCTTCT+AGG + chr8_4:15629404-15629423 None:intergenic 30.0%
GAATCTATTGAGGATGAAAA+AGG - chr8_4:15627158-15627177 Msa1315970:three_prime_UTR 30.0%
TTCTCAATATACCCATCAAT+AGG + chr8_4:15627282-15627301 None:intergenic 30.0%
TGGGTATATTGAGAAGATTA+AGG - chr8_4:15627287-15627306 Msa1315970:three_prime_UTR 30.0%
GTATATTGAGAAGATTAAGG+AGG - chr8_4:15627290-15627309 Msa1315970:three_prime_UTR 30.0%
!! GTTGAACTGAACTGATTTTA+TGG - chr8_4:15627645-15627664 Msa1315970:intron 30.0%
!! ACTGAACTGATTTTATGGTT+CGG - chr8_4:15627650-15627669 Msa1315970:intron 30.0%
! TTTTCAAACATCACTCTGTT+CGG + chr8_4:15627699-15627718 None:intergenic 30.0%
!!! TTTTGGTAGTGTAGATTATC+AGG - chr8_4:15627995-15628014 Msa1315970:intron 30.0%
TACTGTTATGAAACCTTACT+AGG - chr8_4:15628191-15628210 Msa1315970:intron 30.0%
! AGCTTAAAGCATTTCAATGA+TGG - chr8_4:15628272-15628291 Msa1315970:intron 30.0%
! AAAGCATTTCAATGATGGTT+TGG - chr8_4:15628277-15628296 Msa1315970:intron 30.0%
TACTATGATTTCTCATCCTT+AGG + chr8_4:15628378-15628397 None:intergenic 30.0%
AAAATTTCGTGATTGGAATC+TGG + chr8_4:15628787-15628806 None:intergenic 30.0%
CTTCATCAAAATTTCGTGAT+TGG + chr8_4:15628794-15628813 None:intergenic 30.0%
!!! GTGAGAAATTTTTTTGATGG+AGG - chr8_4:15628813-15628832 Msa1315970:intron 30.0%
!! GGTTTTTATGAGTTTAGTTC+AGG - chr8_4:15628938-15628957 Msa1315970:intron 30.0%
TTGAACCAACATTAACAGTT+TGG - chr8_4:15628995-15629014 Msa1315970:intron 30.0%
! GAATGTAGTCAAATTGATTC+TGG - chr8_4:15629095-15629114 Msa1315970:intron 30.0%
AATTCAGTGAAGTCATGTTT+AGG + chr8_4:15629328-15629347 None:intergenic 30.0%
!! TGTAAACTTCTAAAGCTTCT+AGG + chr8_4:15629404-15629423 None:intergenic 30.0%
!! TGTAAACTTCTAAAGCTTCT+AGG + chr8_4:15629404-15629423 None:intergenic 30.0%
!! AGTATAGTAGGGTTTTGATG+AGG - chr8_4:15627106-15627125 Msa1315970:three_prime_UTR 35.0%
TCTATTGAGGATGAAAAAGG+AGG - chr8_4:15627161-15627180 Msa1315970:three_prime_UTR 35.0%
ATAAACCTATTCTCCGGAAA+CGG + chr8_4:15627243-15627262 None:intergenic 35.0%
TCAATATACCCATCAATAGG+CGG + chr8_4:15627279-15627298 None:intergenic 35.0%
ACACTATAATGCCACTACTA+TGG + chr8_4:15627510-15627529 None:intergenic 35.0%
ACCGAACTGTTAAAACAGTT+CGG - chr8_4:15627587-15627606 Msa1315970:intron 35.0%
!!! ACCGAACTGTTTTAACAGTT+CGG + chr8_4:15627591-15627610 None:intergenic 35.0%
AAATCAGTTCAGTTCAACTC+CGG + chr8_4:15627643-15627662 None:intergenic 35.0%
TTGAATCTCTGTTCTTGTGT+TGG + chr8_4:15627762-15627781 None:intergenic 35.0%
! GTTTTGCTCATACTAAACAG+TGG - chr8_4:15627861-15627880 Msa1315970:intron 35.0%
ATCATATTTCAGCACATGAC+AGG - chr8_4:15628035-15628054 Msa1315970:intron 35.0%
CAGCAGAGTAATTGCATAAT+AGG - chr8_4:15628139-15628158 Msa1315970:intron 35.0%
! TTACTAGGTCATGATTTTCC+CGG - chr8_4:15628206-15628225 Msa1315970:intron 35.0%
! TATCTTTTGGATTTCCTTCC+CGG + chr8_4:15628228-15628247 None:intergenic 35.0%
! GTTTTGCTCATACTAAACAG+TGG - chr8_4:15627861-15627880 Msa1315970:intron 35.0%
!! TTGTTGGTAGTGTAGATATC+AGG - chr8_4:15628632-15628651 Msa1315970:intron 35.0%
ATGGAGGTTATTGCAGATTA+AGG - chr8_4:15628829-15628848 Msa1315970:intron 35.0%
CTGAACCAAACTGTTAATGT+TGG + chr8_4:15629003-15629022 None:intergenic 35.0%
! TTTGGTTCAGTTCTACAGTT+TGG - chr8_4:15629013-15629032 Msa1315970:intron 35.0%
! TAGTCAAATTGATTCTGGTC+AGG - chr8_4:15629100-15629119 Msa1315970:intron 35.0%
CATTCAGATATTAGTAAGCC+AGG - chr8_4:15629234-15629253 Msa1315970:exon 35.0%
! TATTTCTGCAAACTAGTCAG+TGG - chr8_4:15629287-15629306 Msa1315970:CDS 35.0%
ATTTCTGCAAACTAGTCAGT+GGG - chr8_4:15629288-15629307 Msa1315970:CDS 35.0%
!! AGTATAGTAGGGTTTTGATG+AGG - chr8_4:15627106-15627125 Msa1315970:three_prime_UTR 35.0%
TCTATTGAGGATGAAAAAGG+AGG - chr8_4:15627161-15627180 Msa1315970:three_prime_UTR 35.0%
ATAAACCTATTCTCCGGAAA+CGG + chr8_4:15627243-15627262 None:intergenic 35.0%
TCAATATACCCATCAATAGG+CGG + chr8_4:15627279-15627298 None:intergenic 35.0%
ACACTATAATGCCACTACTA+TGG + chr8_4:15627510-15627529 None:intergenic 35.0%
ACCGAACTGTTAAAACAGTT+CGG - chr8_4:15627587-15627606 Msa1315970:intron 35.0%
!!! ACCGAACTGTTTTAACAGTT+CGG + chr8_4:15627591-15627610 None:intergenic 35.0%
AAATCAGTTCAGTTCAACTC+CGG + chr8_4:15627643-15627662 None:intergenic 35.0%
TTGAATCTCTGTTCTTGTGT+TGG + chr8_4:15627762-15627781 None:intergenic 35.0%
! GTTTTGCTCATACTAAACAG+TGG - chr8_4:15627861-15627880 Msa1315970:intron 35.0%
ATCATATTTCAGCACATGAC+AGG - chr8_4:15628035-15628054 Msa1315970:intron 35.0%
CAGCAGAGTAATTGCATAAT+AGG - chr8_4:15628139-15628158 Msa1315970:intron 35.0%
! TTACTAGGTCATGATTTTCC+CGG - chr8_4:15628206-15628225 Msa1315970:intron 35.0%
! TATCTTTTGGATTTCCTTCC+CGG + chr8_4:15628228-15628247 None:intergenic 35.0%
! GTTTTGCTCATACTAAACAG+TGG - chr8_4:15627861-15627880 Msa1315970:intron 35.0%
!! TTGTTGGTAGTGTAGATATC+AGG - chr8_4:15628632-15628651 Msa1315970:intron 35.0%
ATGGAGGTTATTGCAGATTA+AGG - chr8_4:15628829-15628848 Msa1315970:intron 35.0%
CTGAACCAAACTGTTAATGT+TGG + chr8_4:15629003-15629022 None:intergenic 35.0%
! TTTGGTTCAGTTCTACAGTT+TGG - chr8_4:15629013-15629032 Msa1315970:intron 35.0%
! TAGTCAAATTGATTCTGGTC+AGG - chr8_4:15629100-15629119 Msa1315970:intron 35.0%
CATTCAGATATTAGTAAGCC+AGG - chr8_4:15629234-15629253 Msa1315970:exon 35.0%
! TATTTCTGCAAACTAGTCAG+TGG - chr8_4:15629287-15629306 Msa1315970:CDS 35.0%
ATTTCTGCAAACTAGTCAGT+GGG - chr8_4:15629288-15629307 Msa1315970:CDS 35.0%
! AATTGGCCAAGGAGTATAGT+AGG - chr8_4:15627094-15627113 Msa1315970:three_prime_UTR 40.0%
! ATTGGCCAAGGAGTATAGTA+GGG - chr8_4:15627095-15627114 Msa1315970:three_prime_UTR 40.0%
CAAAACCCTACTATACTCCT+TGG + chr8_4:15627103-15627122 None:intergenic 40.0%
!! GGTTTTGATGAGGAAGCATA+GGG - chr8_4:15627116-15627135 Msa1315970:three_prime_UTR 40.0%
GTTTCCGGAGAATAGGTTTA+TGG - chr8_4:15627242-15627261 Msa1315970:three_prime_UTR 40.0%
GTTGCCATAAACCTATTCTC+CGG + chr8_4:15627249-15627268 None:intergenic 40.0%
TCAAATAGCGACCATAGTAG+TGG - chr8_4:15627496-15627515 Msa1315970:intron 40.0%
! GTTGCAGTTTTGCGATAACT+AGG - chr8_4:15627548-15627567 Msa1315970:intron 40.0%
CGGTGAAATTGTGAACGATT+CGG + chr8_4:15627623-15627642 None:intergenic 40.0%
CGAATCGTTCACAATTTCAC+CGG - chr8_4:15627621-15627640 Msa1315970:intron 40.0%
! TGTGTTGGTGAGTAATAGTG+CGG + chr8_4:15627747-15627766 None:intergenic 40.0%
ATAACCTGCAACTTAGAGAG+TGG - chr8_4:15627782-15627801 Msa1315970:intron 40.0%
TAACCTGCAACTTAGAGAGT+GGG - chr8_4:15627783-15627802 Msa1315970:intron 40.0%
GGTAAGATTAGCGTCATACA+GGG - chr8_4:15627828-15627847 Msa1315970:intron 40.0%
!!! TAGCAGGCGCATTGTTTTTT+TGG + chr8_4:15627925-15627944 None:intergenic 40.0%
ATATTTCAGCACATGACAGG+TGG - chr8_4:15628038-15628057 Msa1315970:intron 40.0%
GGGAAAATCATGACCTAGTA+AGG + chr8_4:15628207-15628226 None:intergenic 40.0%
! TACTAGGTCATGATTTTCCC+GGG - chr8_4:15628207-15628226 Msa1315970:intron 40.0%
! ATCTTTTGGATTTCCTTCCC+GGG + chr8_4:15628227-15628246 None:intergenic 40.0%
AAGAACAAAGCCCTGTATGA+CGG + chr8_4:15628479-15628498 None:intergenic 40.0%
!!! TCTTTGTTTTCATACCGCAG+TGG - chr8_4:15629175-15629194 Msa1315970:intron 40.0%
!! TTGCGTTAGCTATTGACTTC+TGG - chr8_4:15629263-15629282 Msa1315970:CDS 40.0%
!! TGCGTTAGCTATTGACTTCT+GGG - chr8_4:15629264-15629283 Msa1315970:CDS 40.0%
! AATTGGCCAAGGAGTATAGT+AGG - chr8_4:15627094-15627113 Msa1315970:three_prime_UTR 40.0%
! ATTGGCCAAGGAGTATAGTA+GGG - chr8_4:15627095-15627114 Msa1315970:three_prime_UTR 40.0%
CAAAACCCTACTATACTCCT+TGG + chr8_4:15627103-15627122 None:intergenic 40.0%
!! GGTTTTGATGAGGAAGCATA+GGG - chr8_4:15627116-15627135 Msa1315970:three_prime_UTR 40.0%
GTTTCCGGAGAATAGGTTTA+TGG - chr8_4:15627242-15627261 Msa1315970:three_prime_UTR 40.0%
GTTGCCATAAACCTATTCTC+CGG + chr8_4:15627249-15627268 None:intergenic 40.0%
TCAAATAGCGACCATAGTAG+TGG - chr8_4:15627496-15627515 Msa1315970:intron 40.0%
! GTTGCAGTTTTGCGATAACT+AGG - chr8_4:15627548-15627567 Msa1315970:intron 40.0%
CGGTGAAATTGTGAACGATT+CGG + chr8_4:15627623-15627642 None:intergenic 40.0%
CGAATCGTTCACAATTTCAC+CGG - chr8_4:15627621-15627640 Msa1315970:intron 40.0%
! TGTGTTGGTGAGTAATAGTG+CGG + chr8_4:15627747-15627766 None:intergenic 40.0%
ATAACCTGCAACTTAGAGAG+TGG - chr8_4:15627782-15627801 Msa1315970:intron 40.0%
TAACCTGCAACTTAGAGAGT+GGG - chr8_4:15627783-15627802 Msa1315970:intron 40.0%
GGTAAGATTAGCGTCATACA+GGG - chr8_4:15627828-15627847 Msa1315970:intron 40.0%
!!! TAGCAGGCGCATTGTTTTTT+TGG + chr8_4:15627925-15627944 None:intergenic 40.0%
ATATTTCAGCACATGACAGG+TGG - chr8_4:15628038-15628057 Msa1315970:intron 40.0%
GGGAAAATCATGACCTAGTA+AGG + chr8_4:15628207-15628226 None:intergenic 40.0%
! TACTAGGTCATGATTTTCCC+GGG - chr8_4:15628207-15628226 Msa1315970:intron 40.0%
! ATCTTTTGGATTTCCTTCCC+GGG + chr8_4:15628227-15628246 None:intergenic 40.0%
AAGAACAAAGCCCTGTATGA+CGG + chr8_4:15628479-15628498 None:intergenic 40.0%
!!! TCTTTGTTTTCATACCGCAG+TGG - chr8_4:15629175-15629194 Msa1315970:intron 40.0%
!! TTGCGTTAGCTATTGACTTC+TGG - chr8_4:15629263-15629282 Msa1315970:CDS 40.0%
!! TGCGTTAGCTATTGACTTCT+GGG - chr8_4:15629264-15629283 Msa1315970:CDS 40.0%
!! GGGTTTTGATGAGGAAGCAT+AGG - chr8_4:15627115-15627134 Msa1315970:three_prime_UTR 45.0%
AGGCTATTGAAGCTTTGCCT+GGG - chr8_4:15627181-15627200 Msa1315970:three_prime_UTR 45.0%
AGATTAAGGAGGCTGCTGAT+AGG - chr8_4:15627301-15627320 Msa1315970:three_prime_UTR 45.0%
TCAGTGGGAAGGAGAAGATT+AGG - chr8_4:15627325-15627344 Msa1315970:three_prime_UTR 45.0%
GATTAGGTAGATTGCGTCAC+AGG - chr8_4:15627341-15627360 Msa1315970:three_prime_UTR 45.0%
! GTGTTGGTGAGTAATAGTGC+GGG + chr8_4:15627746-15627765 None:intergenic 45.0%
GGGTAAGATTAGCGTCATAC+AGG - chr8_4:15627827-15627846 Msa1315970:intron 45.0%
CAGTTGAAGGAAGTGATAGC+AGG + chr8_4:15627941-15627960 None:intergenic 45.0%
CTGCTATCACTTCCTTCAAC+TGG - chr8_4:15627939-15627958 Msa1315970:intron 45.0%
GGGAAGATTACCGTCATACA+GGG - chr8_4:15628466-15628485 Msa1315970:intron 45.0%
!!! TAGCAGGCGCGTTGTTTTTT+TGG + chr8_4:15628563-15628582 None:intergenic 45.0%
CAGTTGAAGGAAGTGATAGC+AGG + chr8_4:15627941-15627960 None:intergenic 45.0%
CTGCTATCACTTCCTTCAAC+TGG - chr8_4:15627939-15627958 Msa1315970:intron 45.0%
! CAGTTCGGTTCACACAGTTT+TGG - chr8_4:15628912-15628931 Msa1315970:intron 45.0%
!!! CGGTTCACACAGTTTTGGTT+TGG - chr8_4:15628917-15628936 Msa1315970:intron 45.0%
GTAACTCCAGAACTCCACAA+AGG + chr8_4:15629131-15629150 None:intergenic 45.0%
TGTTACGCAAAACTCCACTG+CGG + chr8_4:15629192-15629211 None:intergenic 45.0%
!! GGGTTTTGATGAGGAAGCAT+AGG - chr8_4:15627115-15627134 Msa1315970:three_prime_UTR 45.0%
AGGCTATTGAAGCTTTGCCT+GGG - chr8_4:15627181-15627200 Msa1315970:three_prime_UTR 45.0%
AGATTAAGGAGGCTGCTGAT+AGG - chr8_4:15627301-15627320 Msa1315970:three_prime_UTR 45.0%
TCAGTGGGAAGGAGAAGATT+AGG - chr8_4:15627325-15627344 Msa1315970:three_prime_UTR 45.0%
GATTAGGTAGATTGCGTCAC+AGG - chr8_4:15627341-15627360 Msa1315970:three_prime_UTR 45.0%
! GTGTTGGTGAGTAATAGTGC+GGG + chr8_4:15627746-15627765 None:intergenic 45.0%
GGGTAAGATTAGCGTCATAC+AGG - chr8_4:15627827-15627846 Msa1315970:intron 45.0%
CAGTTGAAGGAAGTGATAGC+AGG + chr8_4:15627941-15627960 None:intergenic 45.0%
CTGCTATCACTTCCTTCAAC+TGG - chr8_4:15627939-15627958 Msa1315970:intron 45.0%
GGGAAGATTACCGTCATACA+GGG - chr8_4:15628466-15628485 Msa1315970:intron 45.0%
!!! TAGCAGGCGCGTTGTTTTTT+TGG + chr8_4:15628563-15628582 None:intergenic 45.0%
CAGTTGAAGGAAGTGATAGC+AGG + chr8_4:15627941-15627960 None:intergenic 45.0%
CTGCTATCACTTCCTTCAAC+TGG - chr8_4:15627939-15627958 Msa1315970:intron 45.0%
! CAGTTCGGTTCACACAGTTT+TGG - chr8_4:15628912-15628931 Msa1315970:intron 45.0%
!!! CGGTTCACACAGTTTTGGTT+TGG - chr8_4:15628917-15628936 Msa1315970:intron 45.0%
GTAACTCCAGAACTCCACAA+AGG + chr8_4:15629131-15629150 None:intergenic 45.0%
TGTTACGCAAAACTCCACTG+CGG + chr8_4:15629192-15629211 None:intergenic 45.0%
!!! TAATATATATTTTTTTTTGT+TGG - chr8_4:15628616-15628635 Msa1315970:intron 5.0%
!!! TTTAAATATTTAATTTTGAT+TGG - chr8_4:15628704-15628723 Msa1315970:intron 5.0%
!!! TTAAATATTTAATTTTGATT+GGG - chr8_4:15628705-15628724 Msa1315970:intron 5.0%
!!! TAATATATATTTTTTTTTGT+TGG - chr8_4:15628616-15628635 Msa1315970:intron 5.0%
!!! TTTAAATATTTAATTTTGAT+TGG - chr8_4:15628704-15628723 Msa1315970:intron 5.0%
!!! TTAAATATTTAATTTTGATT+GGG - chr8_4:15628705-15628724 Msa1315970:intron 5.0%
GAGGCTATTGAAGCTTTGCC+TGG - chr8_4:15627180-15627199 Msa1315970:three_prime_UTR 50.0%
GGCTATTGAAGCTTTGCCTG+GGG - chr8_4:15627182-15627201 Msa1315970:three_prime_UTR 50.0%
GCTATTGAAGCTTTGCCTGG+GGG - chr8_4:15627183-15627202 Msa1315970:three_prime_UTR 50.0%
GGGTTTGAAGGAAGCTGCTT+TGG - chr8_4:15627203-15627222 Msa1315970:three_prime_UTR 50.0%
TCCGGAAACGGCGTAAAATC+CGG + chr8_4:15627231-15627250 None:intergenic 50.0%
TTACGCCGTTTCCGGAGAAT+AGG - chr8_4:15627235-15627254 Msa1315970:three_prime_UTR 50.0%
AGGCTGCTGATAGGATCAGT+GGG - chr8_4:15627310-15627329 Msa1315970:three_prime_UTR 50.0%
TGCTGATAGGATCAGTGGGA+AGG - chr8_4:15627314-15627333 Msa1315970:three_prime_UTR 50.0%
GCGATAACTAGGCCTGTTCA+TGG - chr8_4:15627559-15627578 Msa1315970:intron 50.0%
GTTCGGTTCAGACCATGAAC+AGG + chr8_4:15627574-15627593 None:intergenic 50.0%
TCTCCCACTCTCTAAGTTGC+AGG + chr8_4:15627789-15627808 None:intergenic 50.0%
! AGGTCATGATTTTCCCGGGA+AGG - chr8_4:15628211-15628230 Msa1315970:intron 50.0%
GGGGAAGATTACCGTCATAC+AGG - chr8_4:15628465-15628484 Msa1315970:intron 50.0%
!! GACTAGCCTTTGTGGAGTTC+TGG - chr8_4:15629122-15629141 Msa1315970:intron 50.0%
GTCGTTGCGTAGCAGTACAT+AGG + chr8_4:15629215-15629234 None:intergenic 50.0%
TAGCTAACGCAAGAGAAGCC+TGG + chr8_4:15629255-15629274 None:intergenic 50.0%
GAGGCTATTGAAGCTTTGCC+TGG - chr8_4:15627180-15627199 Msa1315970:three_prime_UTR 50.0%
GGCTATTGAAGCTTTGCCTG+GGG - chr8_4:15627182-15627201 Msa1315970:three_prime_UTR 50.0%
GCTATTGAAGCTTTGCCTGG+GGG - chr8_4:15627183-15627202 Msa1315970:three_prime_UTR 50.0%
GGGTTTGAAGGAAGCTGCTT+TGG - chr8_4:15627203-15627222 Msa1315970:three_prime_UTR 50.0%
TCCGGAAACGGCGTAAAATC+CGG + chr8_4:15627231-15627250 None:intergenic 50.0%
TTACGCCGTTTCCGGAGAAT+AGG - chr8_4:15627235-15627254 Msa1315970:three_prime_UTR 50.0%
AGGCTGCTGATAGGATCAGT+GGG - chr8_4:15627310-15627329 Msa1315970:three_prime_UTR 50.0%
TGCTGATAGGATCAGTGGGA+AGG - chr8_4:15627314-15627333 Msa1315970:three_prime_UTR 50.0%
GCGATAACTAGGCCTGTTCA+TGG - chr8_4:15627559-15627578 Msa1315970:intron 50.0%
GTTCGGTTCAGACCATGAAC+AGG + chr8_4:15627574-15627593 None:intergenic 50.0%
TCTCCCACTCTCTAAGTTGC+AGG + chr8_4:15627789-15627808 None:intergenic 50.0%
! AGGTCATGATTTTCCCGGGA+AGG - chr8_4:15628211-15628230 Msa1315970:intron 50.0%
GGGGAAGATTACCGTCATAC+AGG - chr8_4:15628465-15628484 Msa1315970:intron 50.0%
!! GACTAGCCTTTGTGGAGTTC+TGG - chr8_4:15629122-15629141 Msa1315970:intron 50.0%
GTCGTTGCGTAGCAGTACAT+AGG + chr8_4:15629215-15629234 None:intergenic 50.0%
TAGCTAACGCAAGAGAAGCC+TGG + chr8_4:15629255-15629274 None:intergenic 50.0%
GAAGCATAGGGCGAGACAAG+CGG - chr8_4:15627128-15627147 Msa1315970:three_prime_UTR 55.0%
CGGCGGAGACGAATCTATTG+AGG - chr8_4:15627148-15627167 Msa1315970:three_prime_UTR 55.0%
AGCTTTGCCTGGGGGTTTGA+AGG - chr8_4:15627191-15627210 Msa1315970:three_prime_UTR 55.0%
! GAAGGAAGCTGCTTTGGTGC+CGG - chr8_4:15627209-15627228 Msa1315970:three_prime_UTR 55.0%
! GCCGGATTTTACGCCGTTTC+CGG - chr8_4:15627227-15627246 Msa1315970:three_prime_UTR 55.0%
ACGCTTACGCCGCCTATTGA+TGG - chr8_4:15627267-15627286 Msa1315970:three_prime_UTR 55.0%
CGCTTACGCCGCCTATTGAT+GGG - chr8_4:15627268-15627287 Msa1315970:three_prime_UTR 55.0%
GAGGCTGCTGATAGGATCAG+TGG - chr8_4:15627309-15627328 Msa1315970:three_prime_UTR 55.0%
!! CTGGTCAGGACTAGCCTTTG+TGG - chr8_4:15629114-15629133 Msa1315970:intron 55.0%
GAAGCATAGGGCGAGACAAG+CGG - chr8_4:15627128-15627147 Msa1315970:three_prime_UTR 55.0%
CGGCGGAGACGAATCTATTG+AGG - chr8_4:15627148-15627167 Msa1315970:three_prime_UTR 55.0%
AGCTTTGCCTGGGGGTTTGA+AGG - chr8_4:15627191-15627210 Msa1315970:three_prime_UTR 55.0%
! GAAGGAAGCTGCTTTGGTGC+CGG - chr8_4:15627209-15627228 Msa1315970:three_prime_UTR 55.0%
! GCCGGATTTTACGCCGTTTC+CGG - chr8_4:15627227-15627246 Msa1315970:three_prime_UTR 55.0%
ACGCTTACGCCGCCTATTGA+TGG - chr8_4:15627267-15627286 Msa1315970:three_prime_UTR 55.0%
CGCTTACGCCGCCTATTGAT+GGG - chr8_4:15627268-15627287 Msa1315970:three_prime_UTR 55.0%
GAGGCTGCTGATAGGATCAG+TGG - chr8_4:15627309-15627328 Msa1315970:three_prime_UTR 55.0%
!! CTGGTCAGGACTAGCCTTTG+TGG - chr8_4:15629114-15629133 Msa1315970:intron 55.0%
GCAGCTTCCTTCAAACCCCC+AGG + chr8_4:15627201-15627220 None:intergenic 60.0%
GCAGCTTCCTTCAAACCCCC+AGG + chr8_4:15627201-15627220 None:intergenic 60.0%
GCATAGGGCGAGACAAGCGG+CGG - chr8_4:15627131-15627150 Msa1315970:three_prime_UTR 65.0%
GCATAGGGCGAGACAAGCGG+CGG - chr8_4:15627131-15627150 Msa1315970:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 15627090 15629496 15627090 ID=Msa1315970;Name=Msa1315970
chr8_4 mRNA 15627090 15629496 15627090 ID=Msa1315970-mRNA-1;Parent=Msa1315970;Name=Msa1315970-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.5|1|0|0|2|387|86
chr8_4 exon 15627090 15627464 15627090 ID=Msa1315970-mRNA-1:exon:6027;Parent=Msa1315970-mRNA-1
chr8_4 exon 15629224 15629496 15629224 ID=Msa1315970-mRNA-1:exon:6026;Parent=Msa1315970-mRNA-1
chr8_4 CDS 15629236 15629496 15629236 ID=Msa1315970-mRNA-1:cds;Parent=Msa1315970-mRNA-1
chr8_4 three_prime_UTR 15629224 15629235 15629224 ID=Msa1315970-mRNA-1:three_prime_utr;Parent=Msa1315970-mRNA-1
chr8_4 three_prime_UTR 15627090 15627464 15627090 ID=Msa1315970-mRNA-1:three_prime_utr;Parent=Msa1315970-mRNA-1
Gene Sequence

>Msa1315970

ATGAAATTGGCCAAGGAGTATAGTAGGGTTTTGATGAGGAAGCATAGGGCGAGACAAGCGGCGGAGACGAATCTATTGAGGATGAAAAAGGAGGCTATTGAAGCTTTGCCTGGGGGTTTGAAGGAAGCTGCTTTGGTGCCGGATTTTACGCCGTTTCCGGAGAATAGGTTTATGGCAACGCTTACGCCGCCTATTGATGGGTATATTGAGAAGATTAAGGAGGCTGCTGATAGGATCAGTGGGAAGGAGAAGATTAGGTAG
Protein sequence

>Msa1315970

MKLAKEYSRVLMRKHRARQAAETNLLRMKKEAIEALPGGLKEAALVPDFTPFPENRFMATLTPPIDGYIEKIKEAADRISGKEKIR*