AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0140300


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0140300 MtrunA17_Chr3g0138531 88.710 186 19 1 10 193 122 307 3.45e-71 218
Msa0140300 MtrunA17_Chr3g0138541 76.923 195 33 2 11 193 122 316 1.73e-43 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0140300 AT2G25670.2 58.919 185 63 4 19 193 137 318 3.84e-17 78.2
Msa0140300 AT2G25670.1 58.919 185 63 4 19 193 137 318 3.84e-17 78.2

Find 35 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCTTGACCTCAGGTTC+AGG 0.291395 1_3:-74551043 None:intergenic
AGCAGCGTCCATTTCTTTAC+TGG 0.341268 1_3:-74551784 None:intergenic
CAGGATAAGAAAGGTGTAGA+TGG 0.373922 1_3:+74551560 Msa0140300:CDS
TCGGCCTCCTTTGGTGCAGC+TGG 0.386362 1_3:-74551076 None:intergenic
AGTTGACGTTCGGCCTCCTT+TGG 0.387948 1_3:-74551085 None:intergenic
CGAACGTCAACTCTCCAAAA+AGG 0.392328 1_3:+74551096 Msa0140300:CDS
TGCTAGAGCTGCTGCTCAAG+AGG 0.430832 1_3:+74551806 Msa0140300:CDS
TCAGCATTCTTCTTGACCTC+AGG 0.431890 1_3:-74551049 None:intergenic
TCAGGTTTCACAGTGTGCTC+TGG 0.433555 1_3:-74551025 None:intergenic
AAATACCTTGGGATTCATCT+TGG 0.436144 1_3:-74551204 None:intergenic
GCACAGGTTGTTGGTTGTAA+TGG 0.445016 1_3:-74551878 None:intergenic
TGTATTTGCAGGTGCACCTC+AGG 0.467621 1_3:+74551541 Msa0140300:intron
GTTAGACTCTTTCACCTCCT+TGG 0.471040 1_3:-74551658 None:intergenic
GTTCTTCTCTTTCTTCTTAG+CGG 0.490710 1_3:-74551856 None:intergenic
TTAAGAAAGTTGCTTCTATG+AGG 0.492845 1_3:+74551751 Msa0140300:CDS
CTGCTCAAGAGGCTGCTGCA+AGG 0.511826 1_3:+74551817 Msa0140300:CDS
AATGCCAAGAAGAGAAAAGA+AGG 0.519050 1_3:+74551626 Msa0140300:CDS
AGGATAAGAAAGGTGTAGAT+GGG 0.528793 1_3:+74551561 Msa0140300:CDS
AAAGAAGGATAAAGCTTCCA+AGG 0.529938 1_3:+74551641 Msa0140300:CDS
AGGCTGCTGCAAGGAACGCA+AGG 0.544412 1_3:+74551826 Msa0140300:CDS
ACACAGAATGAAAGCAATGA+TGG 0.546801 1_3:+74551175 Msa0140300:CDS
GAAGGAACTTGAAGAGCTTG+AGG 0.550464 1_3:+74551129 Msa0140300:CDS
TCTACACCTTTCTTATCCTG+AGG 0.571217 1_3:-74551557 None:intergenic
TGAAGTTCCAGCTGCACCAA+AGG 0.576041 1_3:+74551069 Msa0140300:CDS
TGATGTTGAAAATGCAGAGG+AGG 0.584727 1_3:+74551698 Msa0140300:CDS
CACTGTGAAACCTGAACCTG+AGG 0.592278 1_3:+74551033 Msa0140300:CDS
GGTGCACCTCAGGATAAGAA+AGG 0.599338 1_3:+74551551 Msa0140300:intron
AGTTCCAGCTGCACCAAAGG+AGG 0.599579 1_3:+74551072 Msa0140300:CDS
GGATAAGAAAGGTGTAGATG+GGG 0.604616 1_3:+74551562 Msa0140300:CDS
AATGAAAGCAATGATGGTCA+AGG 0.616215 1_3:+74551181 Msa0140300:CDS
TTCTGATGTTGAAAATGCAG+AGG 0.645244 1_3:+74551695 Msa0140300:CDS
CAAGGCCAAGATGAATCCCA+AGG 0.675177 1_3:+74551199 Msa0140300:CDS
AAGGAGGTGAAAGAGTCTAA+CGG 0.681195 1_3:+74551660 Msa0140300:CDS
ATTACAACCAACAACCTGTG+CGG 0.714310 1_3:+74551880 Msa0140300:CDS
GAAGGATAAAGCTTCCAAGG+AGG 0.726948 1_3:+74551644 Msa0140300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGAAAAAATTATAAATGC+TGG - chr1_3:74551443-74551462 None:intergenic 15.0%
!! TATGAAAAAATTATAAATGC+TGG - chr1_3:74551443-74551462 None:intergenic 15.0%
!! TTACATTATTTGTAGGATTA+TGG + chr1_3:74551245-74551264 Msa0140300:intron 20.0%
!! TTACATTATTTGTAGGATTA+TGG + chr1_3:74551245-74551264 Msa0140300:intron 20.0%
!!! TATGGTTTATTTTTGAGTTC+AGG + chr1_3:74551263-74551282 Msa0140300:intron 25.0%
! GAAAAAATCCAGTAAAGAAA+TGG + chr1_3:74551776-74551795 Msa0140300:CDS 25.0%
!!! TATGGTTTATTTTTGAGTTC+AGG + chr1_3:74551263-74551282 Msa0140300:intron 25.0%
! GAAAAAATCCAGTAAAGAAA+TGG + chr1_3:74551776-74551795 Msa0140300:CDS 25.0%
CTCTGGATAATAAATACCTT+GGG - chr1_3:74551218-74551237 None:intergenic 30.0%
CTGCATCTTACATTATTTGT+AGG + chr1_3:74551238-74551257 Msa0140300:intron 30.0%
TCAGCGAAAAAAGATGAAAA+TGG - chr1_3:74551382-74551401 None:intergenic 30.0%
ATTCGCAAAATTGTATTTGC+AGG + chr1_3:74551530-74551549 Msa0140300:intron 30.0%
! TTATCCTTCTTTTCTCTTCT+TGG - chr1_3:74551633-74551652 None:intergenic 30.0%
!!! TTTTCAACATCAGAAGTGTT+GGG - chr1_3:74551690-74551709 None:intergenic 30.0%
!! ATTTTCAACATCAGAAGTGT+TGG - chr1_3:74551691-74551710 None:intergenic 30.0%
TTAAGAAAGTTGCTTCTATG+AGG + chr1_3:74551751-74551770 Msa0140300:CDS 30.0%
CTCTGGATAATAAATACCTT+GGG - chr1_3:74551218-74551237 None:intergenic 30.0%
CTGCATCTTACATTATTTGT+AGG + chr1_3:74551238-74551257 Msa0140300:intron 30.0%
TCAGCGAAAAAAGATGAAAA+TGG - chr1_3:74551382-74551401 None:intergenic 30.0%
ATTCGCAAAATTGTATTTGC+AGG + chr1_3:74551530-74551549 Msa0140300:intron 30.0%
! TTATCCTTCTTTTCTCTTCT+TGG - chr1_3:74551633-74551652 None:intergenic 30.0%
!!! TTTTCAACATCAGAAGTGTT+GGG - chr1_3:74551690-74551709 None:intergenic 30.0%
!! ATTTTCAACATCAGAAGTGT+TGG - chr1_3:74551691-74551710 None:intergenic 30.0%
TTAAGAAAGTTGCTTCTATG+AGG + chr1_3:74551751-74551770 Msa0140300:CDS 30.0%
! CTTCTTCTTCCTTTCCTTTT+TGG - chr1_3:74551113-74551132 None:intergenic 35.0%
CAAAAAGGAAAGGAAGAAGA+AGG + chr1_3:74551111-74551130 Msa0140300:CDS 35.0%
ACACAGAATGAAAGCAATGA+TGG + chr1_3:74551175-74551194 Msa0140300:CDS 35.0%
AATGAAAGCAATGATGGTCA+AGG + chr1_3:74551181-74551200 Msa0140300:CDS 35.0%
AAATACCTTGGGATTCATCT+TGG - chr1_3:74551207-74551226 None:intergenic 35.0%
! GCTCTGGATAATAAATACCT+TGG - chr1_3:74551219-74551238 None:intergenic 35.0%
AATAATGTAAGATGCAGCTC+TGG - chr1_3:74551235-74551254 None:intergenic 35.0%
!!! TTTCATCTTTTTTCGCTGAC+AGG + chr1_3:74551383-74551402 Msa0140300:intron 35.0%
! AGGATAAGAAAGGTGTAGAT+GGG + chr1_3:74551561-74551580 Msa0140300:CDS 35.0%
! TTTTCTCTTCTTGGCATTCT+TGG - chr1_3:74551624-74551643 None:intergenic 35.0%
AATGCCAAGAAGAGAAAAGA+AGG + chr1_3:74551626-74551645 Msa0140300:CDS 35.0%
AAAGAAGGATAAAGCTTCCA+AGG + chr1_3:74551641-74551660 Msa0140300:CDS 35.0%
TTCTGATGTTGAAAATGCAG+AGG + chr1_3:74551695-74551714 Msa0140300:CDS 35.0%
GTTCTTCTCTTTCTTCTTAG+CGG - chr1_3:74551859-74551878 None:intergenic 35.0%
! CTTCTTCTTCCTTTCCTTTT+TGG - chr1_3:74551113-74551132 None:intergenic 35.0%
CAAAAAGGAAAGGAAGAAGA+AGG + chr1_3:74551111-74551130 Msa0140300:CDS 35.0%
ACACAGAATGAAAGCAATGA+TGG + chr1_3:74551175-74551194 Msa0140300:CDS 35.0%
AATGAAAGCAATGATGGTCA+AGG + chr1_3:74551181-74551200 Msa0140300:CDS 35.0%
AAATACCTTGGGATTCATCT+TGG - chr1_3:74551207-74551226 None:intergenic 35.0%
! GCTCTGGATAATAAATACCT+TGG - chr1_3:74551219-74551238 None:intergenic 35.0%
AATAATGTAAGATGCAGCTC+TGG - chr1_3:74551235-74551254 None:intergenic 35.0%
!!! TTTCATCTTTTTTCGCTGAC+AGG + chr1_3:74551383-74551402 Msa0140300:intron 35.0%
! AGGATAAGAAAGGTGTAGAT+GGG + chr1_3:74551561-74551580 Msa0140300:CDS 35.0%
! TTTTCTCTTCTTGGCATTCT+TGG - chr1_3:74551624-74551643 None:intergenic 35.0%
AATGCCAAGAAGAGAAAAGA+AGG + chr1_3:74551626-74551645 Msa0140300:CDS 35.0%
AAAGAAGGATAAAGCTTCCA+AGG + chr1_3:74551641-74551660 Msa0140300:CDS 35.0%
TTCTGATGTTGAAAATGCAG+AGG + chr1_3:74551695-74551714 Msa0140300:CDS 35.0%
GTTCTTCTCTTTCTTCTTAG+CGG - chr1_3:74551859-74551878 None:intergenic 35.0%
!! CTTTTTGGAGAGTTGACGTT+CGG - chr1_3:74551098-74551117 None:intergenic 40.0%
GTCAACTCTCCAAAAAGGAA+AGG + chr1_3:74551101-74551120 Msa0140300:CDS 40.0%
!! GAGGCTCTTTTAGCTGATTT+TGG + chr1_3:74551148-74551167 Msa0140300:CDS 40.0%
TAGAACGAATGCATGAGCAA+CGG - chr1_3:74551300-74551319 None:intergenic 40.0%
ATGCATTCGTTCTACACTAC+TGG + chr1_3:74551306-74551325 Msa0140300:intron 40.0%
! CTGGTCTTTCTTTCTCTGTT+TGG + chr1_3:74551325-74551344 Msa0140300:intron 40.0%
CTAGATCTTTGAGCAAAAGC+AGG - chr1_3:74551357-74551376 None:intergenic 40.0%
TCTACACCTTTCTTATCCTG+AGG - chr1_3:74551560-74551579 None:intergenic 40.0%
! CAGGATAAGAAAGGTGTAGA+TGG + chr1_3:74551560-74551579 Msa0140300:CDS 40.0%
! GGATAAGAAAGGTGTAGATG+GGG + chr1_3:74551562-74551581 Msa0140300:CDS 40.0%
AAGGAGGTGAAAGAGTCTAA+CGG + chr1_3:74551660-74551679 Msa0140300:CDS 40.0%
TGATGTTGAAAATGCAGAGG+AGG + chr1_3:74551698-74551717 Msa0140300:CDS 40.0%
ATTACAACCAACAACCTGTG+CGG + chr1_3:74551880-74551899 Msa0140300:CDS 40.0%
!! CTTTTTGGAGAGTTGACGTT+CGG - chr1_3:74551098-74551117 None:intergenic 40.0%
GTCAACTCTCCAAAAAGGAA+AGG + chr1_3:74551101-74551120 Msa0140300:CDS 40.0%
!! GAGGCTCTTTTAGCTGATTT+TGG + chr1_3:74551148-74551167 Msa0140300:CDS 40.0%
TAGAACGAATGCATGAGCAA+CGG - chr1_3:74551300-74551319 None:intergenic 40.0%
ATGCATTCGTTCTACACTAC+TGG + chr1_3:74551306-74551325 Msa0140300:intron 40.0%
! CTGGTCTTTCTTTCTCTGTT+TGG + chr1_3:74551325-74551344 Msa0140300:intron 40.0%
CTAGATCTTTGAGCAAAAGC+AGG - chr1_3:74551357-74551376 None:intergenic 40.0%
TCTACACCTTTCTTATCCTG+AGG - chr1_3:74551560-74551579 None:intergenic 40.0%
! CAGGATAAGAAAGGTGTAGA+TGG + chr1_3:74551560-74551579 Msa0140300:CDS 40.0%
! GGATAAGAAAGGTGTAGATG+GGG + chr1_3:74551562-74551581 Msa0140300:CDS 40.0%
AAGGAGGTGAAAGAGTCTAA+CGG + chr1_3:74551660-74551679 Msa0140300:CDS 40.0%
TGATGTTGAAAATGCAGAGG+AGG + chr1_3:74551698-74551717 Msa0140300:CDS 40.0%
ATTACAACCAACAACCTGTG+CGG + chr1_3:74551880-74551899 Msa0140300:CDS 40.0%
TTCTTCTTGACCTCAGGTTC+AGG - chr1_3:74551046-74551065 None:intergenic 45.0%
TCAGCATTCTTCTTGACCTC+AGG - chr1_3:74551052-74551071 None:intergenic 45.0%
CGAACGTCAACTCTCCAAAA+AGG + chr1_3:74551096-74551115 Msa0140300:CDS 45.0%
GAAGGAACTTGAAGAGCTTG+AGG + chr1_3:74551129-74551148 Msa0140300:CDS 45.0%
GTGCATGTCAATCTAGATGC+AGG + chr1_3:74551476-74551495 Msa0140300:intron 45.0%
GAAGGATAAAGCTTCCAAGG+AGG + chr1_3:74551644-74551663 Msa0140300:CDS 45.0%
GTTAGACTCTTTCACCTCCT+TGG - chr1_3:74551661-74551680 None:intergenic 45.0%
AGCAGCGTCCATTTCTTTAC+TGG - chr1_3:74551787-74551806 None:intergenic 45.0%
! GCACAGGTTGTTGGTTGTAA+TGG - chr1_3:74551881-74551900 None:intergenic 45.0%
TTCTTCTTGACCTCAGGTTC+AGG - chr1_3:74551046-74551065 None:intergenic 45.0%
TCAGCATTCTTCTTGACCTC+AGG - chr1_3:74551052-74551071 None:intergenic 45.0%
CGAACGTCAACTCTCCAAAA+AGG + chr1_3:74551096-74551115 Msa0140300:CDS 45.0%
GAAGGAACTTGAAGAGCTTG+AGG + chr1_3:74551129-74551148 Msa0140300:CDS 45.0%
GTGCATGTCAATCTAGATGC+AGG + chr1_3:74551476-74551495 Msa0140300:intron 45.0%
GAAGGATAAAGCTTCCAAGG+AGG + chr1_3:74551644-74551663 Msa0140300:CDS 45.0%
GTTAGACTCTTTCACCTCCT+TGG - chr1_3:74551661-74551680 None:intergenic 45.0%
AGCAGCGTCCATTTCTTTAC+TGG - chr1_3:74551787-74551806 None:intergenic 45.0%
! GCACAGGTTGTTGGTTGTAA+TGG - chr1_3:74551881-74551900 None:intergenic 45.0%
TCAGGTTTCACAGTGTGCTC+TGG - chr1_3:74551028-74551047 None:intergenic 50.0%
CACTGTGAAACCTGAACCTG+AGG + chr1_3:74551033-74551052 Msa0140300:CDS 50.0%
TGAAGTTCCAGCTGCACCAA+AGG + chr1_3:74551069-74551088 Msa0140300:CDS 50.0%
CAAGGCCAAGATGAATCCCA+AGG + chr1_3:74551199-74551218 Msa0140300:CDS 50.0%
TGTATTTGCAGGTGCACCTC+AGG + chr1_3:74551541-74551560 Msa0140300:intron 50.0%
GGTGCACCTCAGGATAAGAA+AGG + chr1_3:74551551-74551570 Msa0140300:intron 50.0%
GGTGTAGATGGGGATGTAGA+TGG + chr1_3:74551572-74551591 Msa0140300:intron 50.0%
TCAGGTTTCACAGTGTGCTC+TGG - chr1_3:74551028-74551047 None:intergenic 50.0%
CACTGTGAAACCTGAACCTG+AGG + chr1_3:74551033-74551052 Msa0140300:CDS 50.0%
TGAAGTTCCAGCTGCACCAA+AGG + chr1_3:74551069-74551088 Msa0140300:CDS 50.0%
CAAGGCCAAGATGAATCCCA+AGG + chr1_3:74551199-74551218 Msa0140300:CDS 50.0%
TGTATTTGCAGGTGCACCTC+AGG + chr1_3:74551541-74551560 Msa0140300:intron 50.0%
GGTGCACCTCAGGATAAGAA+AGG + chr1_3:74551551-74551570 Msa0140300:intron 50.0%
GGTGTAGATGGGGATGTAGA+TGG + chr1_3:74551572-74551591 Msa0140300:intron 50.0%
TGAAGGTGACGAGGAGGTAG+AGG + chr1_3:74550979-74550998 Msa0140300:intron 55.0%
AGTTCCAGCTGCACCAAAGG+AGG + chr1_3:74551072-74551091 Msa0140300:CDS 55.0%
AGTTGACGTTCGGCCTCCTT+TGG - chr1_3:74551088-74551107 None:intergenic 55.0%
TGCTAGAGCTGCTGCTCAAG+AGG + chr1_3:74551806-74551825 Msa0140300:CDS 55.0%
TGAAGGTGACGAGGAGGTAG+AGG + chr1_3:74550979-74550998 Msa0140300:intron 55.0%
AGTTCCAGCTGCACCAAAGG+AGG + chr1_3:74551072-74551091 Msa0140300:CDS 55.0%
AGTTGACGTTCGGCCTCCTT+TGG - chr1_3:74551088-74551107 None:intergenic 55.0%
TGCTAGAGCTGCTGCTCAAG+AGG + chr1_3:74551806-74551825 Msa0140300:CDS 55.0%
CTGCTCAAGAGGCTGCTGCA+AGG + chr1_3:74551817-74551836 Msa0140300:CDS 60.0%
AGGCTGCTGCAAGGAACGCA+AGG + chr1_3:74551826-74551845 Msa0140300:CDS 60.0%
CTGCTCAAGAGGCTGCTGCA+AGG + chr1_3:74551817-74551836 Msa0140300:CDS 60.0%
AGGCTGCTGCAAGGAACGCA+AGG + chr1_3:74551826-74551845 Msa0140300:CDS 60.0%
! TCGGCCTCCTTTGGTGCAGC+TGG - chr1_3:74551079-74551098 None:intergenic 65.0%
! TCGGCCTCCTTTGGTGCAGC+TGG - chr1_3:74551079-74551098 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 74550978 74551905 74550978 ID=Msa0140300;Name=Msa0140300
chr1_3 mRNA 74550978 74551905 74550978 ID=Msa0140300-mRNA-1;Parent=Msa0140300;Name=Msa0140300-mRNA-1;_AED=0.04;_eAED=0.05;_QI=0|0|0|1|1|1|4|0|193
chr1_3 exon 74550978 74550983 74550978 ID=Msa0140300-mRNA-1:exon:25958;Parent=Msa0140300-mRNA-1
chr1_3 exon 74550995 74551220 74550995 ID=Msa0140300-mRNA-1:exon:25959;Parent=Msa0140300-mRNA-1
chr1_3 exon 74551552 74551586 74551552 ID=Msa0140300-mRNA-1:exon:25960;Parent=Msa0140300-mRNA-1
chr1_3 exon 74551591 74551905 74551591 ID=Msa0140300-mRNA-1:exon:25961;Parent=Msa0140300-mRNA-1
chr1_3 CDS 74550978 74550983 74550978 ID=Msa0140300-mRNA-1:cds;Parent=Msa0140300-mRNA-1
chr1_3 CDS 74550995 74551220 74550995 ID=Msa0140300-mRNA-1:cds;Parent=Msa0140300-mRNA-1
chr1_3 CDS 74551552 74551586 74551552 ID=Msa0140300-mRNA-1:cds;Parent=Msa0140300-mRNA-1
chr1_3 CDS 74551591 74551905 74551591 ID=Msa0140300-mRNA-1:cds;Parent=Msa0140300-mRNA-1
Gene Sequence

>Msa0140300

ATGAAGGTAGAGGAAGAGCAAGATCATGAATCAGAACCAGAGCACACTGTGAAACCTGAACCTGAGGTCAAGAAGAATGCTGAAGTTCCAGCTGCACCAAAGGAGGCCGAACGTCAACTCTCCAAAAAGGAAAGGAAGAAGAAGGAACTTGAAGAGCTTGAGGCTCTTTTAGCTGATTTTGGAGTCACACAGAATGAAAGCAATGATGGTCAAGGCCAAGATGAATCCCAAGGTGCACCTCAGGATAAGAAAGGTGTAGATGGGGATATGGCGAAAAGAAAGAAATCACTGCTGAGTCCAAGAATGCCAAGAAGAGAAAAGAAGGATAAAGCTTCCAAGGAGGTGAAAGAGTCTAACGGTCAGCCCAACACTTCTGATGTTGAAAATGCAGAGGAGGATTCATCTGTTGTTGATGTGAAAGAGCGTCTTAAGAAAGTTGCTTCTATGAGGAAGAAAAAATCCAGTAAAGAAATGGACGCTGCTGCTAGAGCTGCTGCTCAAGAGGCTGCTGCAAGGAACGCAAGGCTTGCTGCCGCTAAGAAGAAAGAGAAGAACCATTACAACCAACAACCTGTGCGGTAA

Protein sequence

>Msa0140300

MKVEEEQDHESEPEHTVKPEPEVKKNAEVPAAPKEAERQLSKKERKKKELEELEALLADFGVTQNESNDGQGQDESQGAPQDKKGVDGDMAKRKKSLLSPRMPRREKKDKASKEVKESNGQPNTSDVENAEEDSSVVDVKERLKKVASMRKKKSSKEMDAAARAAAQEAAARNARLAAAKKKEKNHYNQQPVR*