Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1459660 | GER54969.1 | 52.394 | 710 | 251 | 13 | 1 | 627 | 41 | 746 | 0.0 | 689 |
| Msa1459660 | GER54969.1 | 53.665 | 723 | 249 | 9 | 335 | 971 | 24 | 746 | 0.0 | 739 |
| Msa1459660 | GER54969.1 | 58.084 | 334 | 115 | 6 | 1 | 313 | 422 | 751 | 2.20e-112 | 375 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1459660 | sp|Q3ECP6|GDL22_ARATH | 59.229 | 363 | 120 | 2 | 637 | 971 | 29 | 391 | 7.91e-148 | 447 |
| Msa1459660 | sp|Q3ECP6|GDL22_ARATH | 57.022 | 356 | 106 | 5 | 1 | 310 | 42 | 396 | 1.99e-132 | 408 |
| Msa1459660 | sp|Q3ECP6|GDL22_ARATH | 54.817 | 301 | 129 | 2 | 314 | 612 | 4 | 299 | 2.23e-103 | 332 |
| Msa1459660 | sp|Q3ECP6|GDL22_ARATH | 56.667 | 60 | 26 | 0 | 577 | 636 | 341 | 400 | 1.51e-15 | 83.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1459660 | A0A5A7RBH9 | 52.394 | 710 | 251 | 13 | 1 | 627 | 41 | 746 | 0.0 | 689 |
| Msa1459660 | A0A5A7RBH9 | 53.665 | 723 | 249 | 9 | 335 | 971 | 24 | 746 | 0.0 | 739 |
| Msa1459660 | A0A5A7RBH9 | 58.084 | 334 | 115 | 6 | 1 | 313 | 422 | 751 | 1.05e-112 | 375 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0033120 | Msa1459660 | 0.805035 | 1.621050e-49 | -8.615850e-47 |
| Msa0033180 | Msa1459660 | 0.954808 | 1.280597e-112 | -8.615850e-47 |
| Msa0079830 | Msa1459660 | 0.987164 | 2.793516e-169 | -8.615850e-47 |
| Msa0177550 | Msa1459660 | 0.804389 | 2.211903e-49 | -8.615850e-47 |
| Msa0190670 | Msa1459660 | 0.800752 | 1.243784e-48 | -8.615850e-47 |
| Msa0220090 | Msa1459660 | 0.825815 | 3.814773e-54 | -8.615850e-47 |
| Msa0261400 | Msa1459660 | 0.810746 | 9.886498e-51 | -8.615850e-47 |
| Msa0271180 | Msa1459660 | 0.801056 | 1.078165e-48 | -8.615850e-47 |
| Msa0299030 | Msa1459660 | 0.803994 | 2.672324e-49 | -8.615850e-47 |
| Msa0349910 | Msa1459660 | 0.801491 | 8.779833e-49 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1459660 | MtrunA17_Chr1g0191461 | 92.025 | 326 | 8 | 1 | 1 | 308 | 42 | 367 | 0.0 | 612 |
| Msa1459660 | MtrunA17_Chr1g0191461 | 73.900 | 341 | 88 | 1 | 637 | 976 | 29 | 369 | 1.49e-180 | 528 |
| Msa1459660 | MtrunA17_Chr1g0191461 | 66.304 | 368 | 75 | 4 | 314 | 633 | 4 | 370 | 2.12e-159 | 473 |
| Msa1459660 | MtrunA17_Chr7g0273101 | 71.217 | 337 | 95 | 1 | 637 | 971 | 27 | 363 | 3.32e-170 | 501 |
| Msa1459660 | MtrunA17_Chr7g0273101 | 70.695 | 331 | 74 | 5 | 1 | 310 | 40 | 368 | 9.53e-155 | 461 |
| Msa1459660 | MtrunA17_Chr7g0273101 | 60.571 | 350 | 90 | 2 | 335 | 636 | 23 | 372 | 1.14e-139 | 422 |
| Msa1459660 | MtrunA17_Chr8g0381581 | 43.235 | 340 | 183 | 7 | 637 | 969 | 38 | 374 | 8.35e-90 | 292 |
| Msa1459660 | MtrunA17_Chr8g0381581 | 38.587 | 368 | 169 | 9 | 321 | 633 | 17 | 382 | 1.49e-74 | 251 |
| Msa1459660 | MtrunA17_Chr8g0381581 | 41.692 | 331 | 166 | 7 | 1 | 307 | 51 | 378 | 5.07e-74 | 249 |
| Msa1459660 | MtrunA17_Chr1g0160101 | 41.737 | 357 | 196 | 7 | 628 | 977 | 31 | 382 | 1.29e-87 | 286 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1459660 | AT1G54790.1 | 63.798 | 337 | 120 | 1 | 637 | 971 | 29 | 365 | 6.66e-155 | 462 |
| Msa1459660 | AT1G54790.1 | 60.909 | 330 | 108 | 3 | 1 | 310 | 42 | 370 | 2.13e-139 | 422 |
| Msa1459660 | AT1G54790.1 | 51.213 | 371 | 133 | 2 | 314 | 636 | 4 | 374 | 2.13e-126 | 388 |
| Msa1459660 | AT1G54790.3 | 62.315 | 337 | 125 | 1 | 637 | 971 | 30 | 366 | 1.91e-151 | 453 |
| Msa1459660 | AT1G54790.3 | 60.606 | 330 | 109 | 3 | 1 | 310 | 43 | 371 | 5.83e-138 | 419 |
| Msa1459660 | AT1G54790.3 | 50.000 | 368 | 134 | 3 | 319 | 636 | 8 | 375 | 1.94e-123 | 381 |
| Msa1459660 | AT1G54790.2 | 59.229 | 363 | 120 | 2 | 637 | 971 | 29 | 391 | 8.04e-149 | 447 |
| Msa1459660 | AT1G54790.2 | 57.022 | 356 | 106 | 5 | 1 | 310 | 42 | 396 | 2.03e-133 | 408 |
| Msa1459660 | AT1G54790.2 | 54.817 | 301 | 129 | 2 | 314 | 612 | 4 | 299 | 2.27e-104 | 332 |
| Msa1459660 | AT1G54790.2 | 56.667 | 60 | 26 | 0 | 577 | 636 | 341 | 400 | 1.53e-16 | 83.6 |
Find 231 sgRNAs with CRISPR-Local
Find 860 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCAAAGATTGATGAACT+AGG | 0.554215 | tig0040397:+1290 | Msa1459660:CDS |
| AAATGAGCCAAGCATCCGAG+TGG | 0.678410 | tig0040397:-14757 | None:intergenic |
| AAATGCCTATCTGGATTCAG+TGG | 0.553590 | tig0040397:+625 | Msa1459660:CDS |
| AAATGGGCCAAGCATCCGAG+TGG | 0.678410 | tig0040397:-10427 | None:intergenic |
| AACAAAGGTCTTAAATGGGA+CGG | 0.594075 | tig0040397:+15144 | Msa1459660:CDS |
| AACACGTCCACCTGTGTCCG+AGG | 0.613374 | tig0040397:-13267 | None:intergenic |
| AACATATTGATTTGCAGCCT+CGG | 0.517902 | tig0040397:-1797 | None:intergenic |
| AACATATTGATTTGTGGCCT+CGG | 0.463948 | tig0040397:-10901 | None:intergenic |
| AAGGTTCTTCAAGTCCTTGC+AGG | 0.403634 | tig0040397:+776 | Msa1459660:CDS |
| AAGTGATTCTGTCCGTAAGG+TGG | 0.517379 | tig0040397:-13314 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTAAGAAGTCAACAATGAGA+CGG | - | tig0040397:126-145 | None:intergenic | 30.0% | |
| TTGCTTGAGAATTAGACTAA+GGG | + | tig0040397:230-249 | Msa1459660:intron | 30.0% | |
| TCCTTAGATAAAAGATGTCT+CGG | - | tig0040397:293-312 | None:intergenic | 30.0% | |
| TTGGCTCATATTTGTAATTG+TGG | - | tig0040397:456-475 | None:intergenic | 30.0% | |
| ACATCTGATGTTACATACAT+AGG | + | tig0040397:484-503 | Msa1459660:intron | 30.0% | |
| CATCTGATGTTACATACATA+GGG | + | tig0040397:485-504 | Msa1459660:intron | 30.0% | |
| AAGCATACAAAACTTGTGTT+TGG | + | tig0040397:513-532 | Msa1459660:intron | 30.0% | |
| CTTGTGTTTGGAAATAAGAA+AGG | + | tig0040397:525-544 | Msa1459660:intron | 30.0% | |
| AGTTCCATTTGATGTGATTT+CGG | + | tig0040397:564-583 | Msa1459660:intron | 30.0% | |
| TAAACCGAAATCACATCAAA+TGG | - | tig0040397:571-590 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0040397 | gene | 31 | 15303 | 31 | ID=Msa1459660;Name=Msa1459660 |
| tig0040397 | mRNA | 31 | 15303 | 31 | ID=Msa1459660-mRNA-1;Parent=Msa1459660;Name=Msa1459660-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0.35|0.13|1|0.78|0.6|15|0|979 |
| tig0040397 | exon | 31 | 145 | 31 | ID=Msa1459660-mRNA-1:exon:22348;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 600 | 797 | 600 | ID=Msa1459660-mRNA-1:exon:22349;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 891 | 1049 | 891 | ID=Msa1459660-mRNA-1:exon:22350;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 1243 | 1467 | 1243 | ID=Msa1459660-mRNA-1:exon:22351;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 1629 | 1860 | 1629 | ID=Msa1459660-mRNA-1:exon:22352;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 9112 | 9350 | 9112 | ID=Msa1459660-mRNA-1:exon:22353;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 9782 | 9979 | 9782 | ID=Msa1459660-mRNA-1:exon:22354;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 10087 | 10253 | 10087 | ID=Msa1459660-mRNA-1:exon:22355;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 10379 | 10572 | 10379 | ID=Msa1459660-mRNA-1:exon:22356;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 10797 | 10976 | 10797 | ID=Msa1459660-mRNA-1:exon:22357;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 13235 | 13389 | 13235 | ID=Msa1459660-mRNA-1:exon:22358;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 14108 | 14305 | 14108 | ID=Msa1459660-mRNA-1:exon:22359;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 14416 | 14582 | 14416 | ID=Msa1459660-mRNA-1:exon:22360;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 14709 | 14979 | 14709 | ID=Msa1459660-mRNA-1:exon:22361;Parent=Msa1459660-mRNA-1 |
| tig0040397 | exon | 15065 | 15303 | 15065 | ID=Msa1459660-mRNA-1:exon:22362;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 31 | 145 | 31 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 600 | 797 | 600 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 891 | 1049 | 891 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 1243 | 1467 | 1243 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 1629 | 1860 | 1629 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 9112 | 9350 | 9112 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 9782 | 9979 | 9782 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 10087 | 10253 | 10087 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 10379 | 10572 | 10379 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 10797 | 10976 | 10797 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 13235 | 13389 | 13235 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 14108 | 14305 | 14108 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 14416 | 14582 | 14416 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 14709 | 14979 | 14709 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| tig0040397 | CDS | 15065 | 15303 | 15065 | ID=Msa1459660-mRNA-1:cds;Parent=Msa1459660-mRNA-1 |
| Gene Sequence |
>Msa1459660 ACAGGTGAACTTTGTGCAGCCAAGGGCTTTCAACCCGCCCCACCCAACGGACAAAATTACTTCAAAACTCCAGCTGGTAGATTTTGCGATGGCCGTCTCATTGTTGACTTCTTAATGGATGCAATGGACTTGCCATTCTTAAATGCCTATCTGGATTCAGTGGGCTCGCCAAATTTCCATCACGGATGCAACTTTGCAGCAGCAGGTTCAACTATCCTTCCAGCAAATGCAGCATCAATCAGTCCATTCGGATTTGGCACTCAGGTAAATCAGTTTCTGCTATTCAAAGCCAAGGTTCTTCAAGTCCTTGCAGGTAAGAAATTTGATAAATATGTCCCAGCTGAAGACTATTTCCAGAAGGGGTTATACATGTTTGACATAGGCCAAAACGATCTTGCTGGTGCATTTTATTCAAAAGACTTGGATCAAATACTTTCCTCTATTCCAACAATTCTACTGGAATTTGAAACTGGTCCACTCGGATGCTTGGGTCAAAATGTTGCCACATTTGGCCATGATAAATCAAAGATTGATGAACTAGGATGTCTTCGTGCACACAACGAAGCTGCTAAGGCCTTTAATCTACAGTTGCAAGCTTTGTGGGCTAAATTGCAGGGCCAGTATCTAGATCTAAATGTTACATATGTTGATATCTTTACCATAAAATTAGACCTCATTGCAAACTACTCAAAACATGGGTTTGAACAACCTTTTATGGCTTGTTGTGGATATGGAGGTCCGCCATTTAACTACGACAGTCGCGTTTCTTGTGGAATAACAACAACAATCTTGAATGGGACCACAATTACAGCAAAAGGTTGCAATGATAGCGGAGTGTATGTAAGCTGGGACGGAACTCATTACACCGAGGCTGCAAATCAATATGTTGCATCACAAATTCTCACCAGACACTACTCCAACACTCATTTTGACATGGATTCTAAGTATGTTGCTTTACGAGTTGTGCTATTTTGCATGTGTTTGGTTGTGGCAAATGCTGTTGTTGACTTCAGTTACCCGGCAGTTTTCAACTTTGGTGATTCAAACTCAGACACAGGTGGACGTGTTGCAGCGTTCGGCAATCAACTGCCCCCACCTTACGGACAAAATCACTTCAAAACTCCGTCTGGGAGATTCTGCGATGGTCGTCTCATGCTTGACTTCTTAATGAATGCAACGGACTTGCCATTCTTAAATGCCTATATGGATTCATTTGGTTTGCCGAATTTCCATCAAGGATGCAACTTTGCTGCATCAGGTTCATCTATCCTACCGGCAACTGGAGCACGGATCAGCCCGTTCAGCTTTGGGGTTCAGGTATCTCAGTTTCTGCTATTCAAAGCTCGGGTTCTCGAATTACTTGCAGGCAAGAAATTTGACGAGTATGTCCCAGCCGAAGATTATTTCGAGAAGGGGTTATACACTTTTGACATTGGTCAGAACGATTTAACCATCGCATTTTATTCACAAGACTTGGACCAAATAATTGCCTCAATTCCAACAATTCTATTGGAATTTGAAACTGGAATAAAGAGACTGTACGACAGCGGGGCTAGAAATTTTTGGATACATAACACGAGTCCACTCGGATGCTTGGCCCATTTTATTGCCCAATTTGGCACTGACCCATCAAAGCTTGATGAACTAGGATGTCTTCAAGAACACAACCAAGCTTCTAAAGCCTTTAATCTACATCTGCAAGATTTCTGCAGTAAATTGCAGGGCCATCGTGTATCTTGTGGAGAAACAAGGGTCTTGAATGGGACCATTGTTACAGCAAAAGGTTGCAATGATAGTAGTGTGTATGTAAGTTGGGACGGGGCTCATTTTACCGAGGCCACAAATCAATATGTTGCATCACAAATTCTCACTGGAAATTACATCAACACTCATTTGTCAGAAAAGATTTATCCAACAGCTTTCAACTTTGGTGATTCAACCTCGGACACAGGTGGACGTGTTGCAGCCTTTGGCCTTCCACTCCCCCCACCTTACGGACAGAATCACTTCAAAACTCCGTCTGGGAGATTTTGTGATGGTCGTCTCATACTTGACTTCTTAATGAATGCAACGGACTTGCCATTCTTAAATGCCTATTTGGATTCATTTGGCTTGCCAAATTTCCATCATGGATGCAACTTTGCAGCATCAGGTTCATCTATTCTTCCACCAACTGCAGCGCGGATCAGTCCGTTCAGCTTTGGGGTTCAGGTATCTCAATTTCTGCTATTCAAAGCTCGGGTTCTCAAATTACTTGCAGGCCAAAAAATTTACGAGTATGACCCGGCCAAAGATTATTTCGAGAAGGGGTTATACATTTTTGAAATAGGTCGGAATGATCTTACTATCGCGTTTTATTCACAAGACTTGGACCAGATAATTGCCTCAATTCCAACAATTCTATTGGAATTTGAAACTGGAATAAAGAGACTGTATGACAGCGGGGCTAGGAATTTTTGGATACATAATGTGAGTCCACTCGGATGCTTGGCTCATTTTATTTCCCAATTTGGCACTGATCCATCAAAGCTTGATGAACTAGGATGCCTTCGTGAACACAACTTAGCTGCTAAATCTTTTAATCTATATCTACAAGATTTCTGTAGTAAATTGCATGGCCAGTATCCAGATGTAAATGTTACATATGTTGATATCTTTACCATAAAACTAGACCTCATTGCAAATTACTCACAACATGGGTTTGAACAGCCAATTATGGCTTGTTGTGGATATGGAGGTCCGCCGTTGAACTATGATAGTCGCGTTTCTTGTGGAGAAACAAAGGTCTTAAATGGGACGGTAGTTACAGCAAAAGGTTGCAATGATAGTAGTGTGTATGTAAGCTGGGATGGCGCTCATTTTACGGAGGCCACAAATCGATATGTTGCATCACAAATTCTCACTGGAAATTACATTAACACTCATTTGCCAGAAAAG |
| Protein sequence |
>Msa1459660 TGELCAAKGFQPAPPNGQNYFKTPAGRFCDGRLIVDFLMDAMDLPFLNAYLDSVGSPNFHHGCNFAAAGSTILPANAASISPFGFGTQVNQFLLFKAKVLQVLAGKKFDKYVPAEDYFQKGLYMFDIGQNDLAGAFYSKDLDQILSSIPTILLEFETGPLGCLGQNVATFGHDKSKIDELGCLRAHNEAAKAFNLQLQALWAKLQGQYLDLNVTYVDIFTIKLDLIANYSKHGFEQPFMACCGYGGPPFNYDSRVSCGITTTILNGTTITAKGCNDSGVYVSWDGTHYTEAANQYVASQILTRHYSNTHFDMDSKYVALRVVLFCMCLVVANAVVDFSYPAVFNFGDSNSDTGGRVAAFGNQLPPPYGQNHFKTPSGRFCDGRLMLDFLMNATDLPFLNAYMDSFGLPNFHQGCNFAASGSSILPATGARISPFSFGVQVSQFLLFKARVLELLAGKKFDEYVPAEDYFEKGLYTFDIGQNDLTIAFYSQDLDQIIASIPTILLEFETGIKRLYDSGARNFWIHNTSPLGCLAHFIAQFGTDPSKLDELGCLQEHNQASKAFNLHLQDFCSKLQGHRVSCGETRVLNGTIVTAKGCNDSSVYVSWDGAHFTEATNQYVASQILTGNYINTHLSEKIYPTAFNFGDSTSDTGGRVAAFGLPLPPPYGQNHFKTPSGRFCDGRLILDFLMNATDLPFLNAYLDSFGLPNFHHGCNFAASGSSILPPTAARISPFSFGVQVSQFLLFKARVLKLLAGQKIYEYDPAKDYFEKGLYIFEIGRNDLTIAFYSQDLDQIIASIPTILLEFETGIKRLYDSGARNFWIHNVSPLGCLAHFISQFGTDPSKLDELGCLREHNLAAKSFNLYLQDFCSKLHGQYPDVNVTYVDIFTIKLDLIANYSQHGFEQPIMACCGYGGPPLNYDSRVSCGETKVLNGTVVTAKGCNDSSVYVSWDGAHFTEATNRYVASQILTGNYINTHLPEK |
