AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0177550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0177550 MtrunA17_Chr1g0200491 98.701 154 2 0 270 423 219 372 1.03e-107 322
Msa0177550 MtrunA17_Chr1g0200491 66.932 251 50 4 31 269 135 364 3.73e-103 311
Msa0177550 MtrunA17_Chr1g0200491 91.538 130 4 1 1 123 1 130 1.01e-73 235
Msa0177550 MtrunA17_Chr1g0163601 42.623 427 174 10 7 423 5 370 3.58e-98 298
Msa0177550 MtrunA17_Chr1g0163611 40.654 428 178 11 9 423 6 370 3.00e-93 285
Msa0177550 MtrunA17_Chr7g0218131 69.481 154 47 0 270 423 217 370 4.85e-74 236
Msa0177550 MtrunA17_Chr7g0218131 69.655 145 44 0 123 267 216 360 6.48e-70 225
Msa0177550 MtrunA17_Chr7g0218131 59.231 130 45 2 1 124 1 128 3.01e-37 139
Msa0177550 MtrunA17_Chr2g0307521 66.883 154 51 0 270 423 210 363 2.54e-72 231
Msa0177550 MtrunA17_Chr2g0307521 67.586 145 47 0 123 267 209 353 3.65e-69 223
Msa0177550 MtrunA17_Chr2g0307521 56.881 109 40 2 21 124 15 121 2.64e-27 111
Msa0177550 MtrunA17_Chr7g0218261 69.481 154 47 0 270 423 215 368 9.73e-72 230
Msa0177550 MtrunA17_Chr7g0218261 68.493 146 46 0 123 268 214 359 2.74e-67 218
Msa0177550 MtrunA17_Chr7g0218261 57.600 125 45 2 8 126 7 129 4.20e-34 130
Msa0177550 MtrunA17_Chr7g0218271 68.182 154 49 0 270 423 222 375 3.21e-71 229
Msa0177550 MtrunA17_Chr7g0218271 67.123 146 48 0 123 268 221 366 9.76e-67 217
Msa0177550 MtrunA17_Chr7g0218271 49.242 132 56 2 1 122 1 131 8.07e-30 119
Msa0177550 MtrunA17_Chr7g0218301 68.182 154 49 0 270 423 222 375 1.22e-70 227
Msa0177550 MtrunA17_Chr7g0218301 67.123 146 48 0 123 268 221 366 3.70e-66 216
Msa0177550 MtrunA17_Chr7g0218301 52.273 132 52 2 1 122 1 131 1.95e-32 126
Msa0177550 MtrunA17_Chr7g0218181 65.584 154 53 0 270 423 214 367 2.59e-68 221
Msa0177550 MtrunA17_Chr7g0218181 65.517 145 50 0 123 267 213 357 2.91e-64 210
Msa0177550 MtrunA17_Chr7g0218181 59.483 116 43 2 8 121 8 121 7.13e-33 127
Msa0177550 MtrunA17_Chr3g0085201 66.234 154 51 1 270 423 205 357 1.75e-65 213
Msa0177550 MtrunA17_Chr3g0085201 65.986 147 49 1 123 269 204 349 2.09e-61 202
Msa0177550 MtrunA17_Chr3g0085201 53.846 117 47 3 13 123 1 116 1.42e-28 115
Msa0177550 MtrunA17_Chr1g0163621 37.615 436 182 16 12 423 15 384 1.91e-65 214
Msa0177550 MtrunA17_Chr7g0218211 61.688 154 59 0 270 423 216 369 5.14e-65 212
Msa0177550 MtrunA17_Chr7g0218211 60.959 146 57 0 123 268 215 360 5.63e-61 202
Msa0177550 MtrunA17_Chr7g0218211 57.500 120 43 2 8 121 7 124 2.80e-33 128
Msa0177550 MtrunA17_Chr3g0085211 56.647 173 73 2 251 423 200 370 2.24e-61 203
Msa0177550 MtrunA17_Chr3g0085211 58.503 147 60 1 123 269 217 362 2.08e-56 190
Msa0177550 MtrunA17_Chr3g0085211 51.562 128 56 3 21 143 22 148 2.69e-29 117
Msa0177550 MtrunA17_Chr3g0128011 54.194 155 69 2 270 423 264 417 8.36e-54 184
Msa0177550 MtrunA17_Chr3g0128011 54.054 148 67 1 123 269 263 410 6.81e-53 182
Msa0177550 MtrunA17_Chr3g0128011 47.863 117 52 3 10 120 60 173 8.94e-21 94.0
Msa0177550 MtrunA17_Chr7g0276411 32.220 419 216 13 13 423 4 362 1.93e-50 174
Msa0177550 MtrunA17_Chr5g0448091 52.903 155 71 2 270 423 210 363 6.17e-50 173
Msa0177550 MtrunA17_Chr5g0448091 53.741 147 67 1 123 268 209 355 2.95e-49 171
Msa0177550 MtrunA17_Chr5g0448091 41.121 107 56 2 20 120 14 119 6.61e-15 75.9
Msa0177550 MtrunA17_Chr8g0367451 31.401 414 216 12 18 423 23 376 2.33e-47 166
Msa0177550 MtrunA17_Chr7g0276401 51.613 155 73 2 270 423 222 375 1.71e-46 164
Msa0177550 MtrunA17_Chr7g0276401 52.448 143 67 1 123 264 221 363 1.59e-44 159
Msa0177550 MtrunA17_Chr8g0345851 50.000 156 76 2 269 423 136 290 5.60e-45 157
Msa0177550 MtrunA17_Chr8g0345851 52.448 143 67 1 123 264 136 278 2.48e-44 156
Msa0177550 MtrunA17_Chr8g0367441 49.032 155 77 2 270 423 224 377 3.14e-43 155
Msa0177550 MtrunA17_Chr8g0367441 49.650 143 71 1 123 264 223 365 1.14e-41 151
Msa0177550 MtrunA17_Chr8g0367491 45.806 155 82 2 270 423 223 376 5.63e-42 152
Msa0177550 MtrunA17_Chr8g0367491 46.154 143 76 1 123 264 222 364 2.04e-40 147
Msa0177550 MtrunA17_Chr8g0367501 48.718 156 78 2 270 423 210 365 7.79e-41 150
Msa0177550 MtrunA17_Chr8g0367501 48.649 148 75 1 123 269 209 356 1.84e-40 149
Msa0177550 MtrunA17_Chr8g0367381 50.407 123 60 1 123 244 204 326 2.64e-35 133
Msa0177550 MtrunA17_Chr8g0367381 50.000 122 60 1 270 390 205 326 1.84e-34 130
Msa0177550 MtrunA17_Chr6g0482291 40.385 156 83 3 269 423 30 176 3.29e-35 128
Msa0177550 MtrunA17_Chr6g0482291 40.816 147 78 2 123 268 30 168 6.62e-34 124
Msa0177550 MtrunA17_Chr3g0121301 45.652 138 72 2 123 257 205 342 1.23e-34 132
Msa0177550 MtrunA17_Chr3g0121301 44.366 142 76 2 270 408 206 347 6.12e-34 130
Msa0177550 MtrunA17_Chr4g0021981 44.681 141 77 1 123 262 214 354 2.64e-33 128
Msa0177550 MtrunA17_Chr4g0021981 43.571 140 78 1 270 408 215 354 2.97e-32 125
Msa0177550 MtrunA17_Chr4g0022021 44.828 145 71 5 123 262 214 354 6.55e-33 127
Msa0177550 MtrunA17_Chr4g0022021 41.772 158 82 6 256 408 202 354 2.22e-32 125
Msa0177550 MtrunA17_Chr3g0130061 39.779 181 98 5 249 423 183 358 1.59e-31 123
Msa0177550 MtrunA17_Chr3g0130061 42.953 149 78 3 123 268 202 346 4.95e-31 122
Msa0177550 MtrunA17_Chr3g0130011 38.674 181 100 5 249 423 183 358 4.61e-31 122
Msa0177550 MtrunA17_Chr3g0130011 41.611 149 80 3 123 268 202 346 3.60e-30 119
Msa0177550 MtrunA17_Chr2g0314271 42.361 144 77 4 123 261 197 339 9.79e-31 121
Msa0177550 MtrunA17_Chr2g0314271 40.252 159 88 5 270 423 198 354 6.37e-30 119
Msa0177550 MtrunA17_Chr7g0223241 39.161 143 84 1 123 262 194 336 1.11e-29 118
Msa0177550 MtrunA17_Chr7g0223241 38.028 142 85 1 270 408 195 336 1.34e-28 115
Msa0177550 MtrunA17_Chr4g0046531 39.860 143 83 1 123 262 562 704 2.39e-28 117
Msa0177550 MtrunA17_Chr4g0046531 37.179 156 94 2 270 422 563 717 1.61e-27 115
Msa0177550 MtrunA17_Chr4g0046531 37.762 143 86 1 123 262 200 342 2.81e-27 114
Msa0177550 MtrunA17_Chr4g0046531 36.620 142 87 1 270 408 201 342 2.75e-26 111
Msa0177550 MtrunA17_Chr5g0432251 34.637 179 107 4 91 263 172 346 3.68e-28 114
Msa0177550 MtrunA17_Chr5g0432251 36.301 146 85 3 270 409 203 346 3.77e-26 108
Msa0177550 MtrunA17_Chr1g0188391 38.194 144 84 3 123 262 205 347 9.26e-28 113
Msa0177550 MtrunA17_Chr1g0188391 37.063 143 85 3 270 408 206 347 9.89e-27 110
Msa0177550 MtrunA17_Chr5g0432261 35.417 144 90 1 123 263 171 314 2.23e-27 111
Msa0177550 MtrunA17_Chr5g0432261 34.266 143 91 1 270 409 172 314 2.39e-26 108
Msa0177550 MtrunA17_Chr5g0432301 38.095 147 83 3 123 263 203 347 7.36e-27 110
Msa0177550 MtrunA17_Chr5g0432301 36.986 146 84 3 270 409 204 347 8.49e-26 107
Msa0177550 MtrunA17_Chr7g0223231 37.063 143 87 1 123 262 194 336 8.88e-27 110
Msa0177550 MtrunA17_Chr7g0223231 35.915 142 88 1 270 408 195 336 1.06e-25 107
Msa0177550 MtrunA17_Chr7g0219141 35.417 144 89 2 123 263 202 344 9.22e-27 110
Msa0177550 MtrunA17_Chr7g0219141 34.266 143 90 2 270 409 203 344 1.11e-25 107
Msa0177550 MtrunA17_Chr7g0219161 34.722 144 90 2 123 263 202 344 2.48e-26 108
Msa0177550 MtrunA17_Chr7g0219161 33.566 143 91 2 270 409 203 344 2.90e-25 105
Msa0177550 MtrunA17_Chr5g0432241 33.520 179 109 4 91 263 173 347 5.50e-26 108
Msa0177550 MtrunA17_Chr5g0432241 34.932 146 87 3 270 409 204 347 1.57e-24 103
Msa0177550 MtrunA17_Chr1g0170931 50.909 110 43 2 26 126 6 113 1.54e-25 100
Msa0177550 MtrunA17_Chr8g0367461 41.593 113 64 2 312 423 1 112 4.61e-25 99.0
Msa0177550 MtrunA17_Chr8g0367461 40.385 104 61 1 166 268 1 104 1.28e-22 92.4
Msa0177550 MtrunA17_Chr5g0432211 32.961 179 110 4 91 263 158 332 1.43e-24 103
Msa0177550 MtrunA17_Chr5g0432211 34.247 146 88 3 270 409 189 332 9.44e-23 98.6
Msa0177550 MtrunA17_Chr1g0188371 36.842 133 80 2 123 251 203 335 2.58e-24 103
Msa0177550 MtrunA17_Chr1g0188371 36.364 132 80 2 270 397 204 335 1.76e-23 101
Msa0177550 MtrunA17_Chr4g0046361 37.500 144 87 1 123 263 205 348 1.59e-23 100
Msa0177550 MtrunA17_Chr4g0046361 28.205 390 214 14 34 416 26 356 2.95e-23 100
Msa0177550 MtrunA17_Chr4g0040621 36.301 146 86 3 123 263 213 356 1.90e-23 100
Msa0177550 MtrunA17_Chr4g0040621 35.172 145 87 3 270 409 214 356 1.91e-22 98.2
Msa0177550 MtrunA17_Chr7g0219171 28.947 228 132 8 41 263 33 235 3.35e-22 95.1
Msa0177550 MtrunA17_Chr7g0219171 34.167 120 75 2 293 409 117 235 4.50e-21 92.0
Msa0177550 MtrunA17_Chr4g0046341 37.226 137 83 1 130 263 215 351 3.63e-22 97.1
Msa0177550 MtrunA17_Chr4g0046341 36.552 145 88 2 276 416 215 359 3.70e-22 97.1
Msa0177550 MtrunA17_Chr4g0014561 38.889 144 85 1 123 263 207 350 6.25e-21 93.6
Msa0177550 MtrunA17_Chr4g0014561 37.762 143 86 1 270 409 208 350 6.28e-20 90.9
Msa0177550 MtrunA17_Chr4g0014681 38.889 144 85 1 123 263 207 350 9.78e-21 93.2
Msa0177550 MtrunA17_Chr4g0014681 37.762 143 86 1 270 409 208 350 9.70e-20 90.1
Msa0177550 MtrunA17_Chr4g0014641 37.500 144 87 1 123 263 207 350 8.18e-20 90.5
Msa0177550 MtrunA17_Chr4g0014641 36.364 143 88 1 270 409 208 350 7.93e-19 87.4
Msa0177550 MtrunA17_Chr8g0367401 62.069 58 22 0 123 180 54 111 3.56e-19 83.6
Msa0177550 MtrunA17_Chr8g0367401 60.345 58 23 0 269 326 54 111 2.38e-18 81.3
Msa0177550 MtrunA17_Chr1g0170951 47.945 73 38 0 188 260 17 89 4.28e-18 79.3
Msa0177550 MtrunA17_Chr1g0170951 47.945 73 38 0 334 406 17 89 4.28e-18 79.3
Msa0177550 MtrunA17_Chr8g0367391 35.772 123 72 3 302 423 18 134 5.79e-17 77.0
Msa0177550 MtrunA17_Chr8g0367391 35.455 110 65 2 156 264 18 122 1.33e-14 70.5
Msa0177550 MtrunA17_Chr1g0170941 63.636 55 20 0 123 177 22 76 1.56e-16 73.9
Msa0177550 MtrunA17_Chr1g0170941 60.714 56 22 0 268 323 21 76 8.01e-16 72.0
Msa0177550 MtrunA17_Chr6g0476581 52.941 85 32 3 8 86 8 90 2.73e-16 74.7
Msa0177550 MtrunA17_Chr4g0014661 37.762 143 86 1 123 262 201 343 4.32e-15 76.3
Msa0177550 MtrunA17_Chr4g0014661 36.620 142 87 1 270 408 202 343 4.36e-14 73.2
Msa0177550 MtrunA17_Chr4g0014591 37.063 143 87 1 123 262 201 343 1.38e-13 71.6
Msa0177550 MtrunA17_Chr4g0014591 35.915 142 88 1 270 408 202 343 1.39e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0177550 AT5G54160.1 56.164 146 63 1 123 267 207 352 2.73e-53 182
Msa0177550 AT5G54160.1 56.552 145 62 1 270 413 208 352 1.33e-52 180
Msa0177550 AT5G54160.1 49.533 107 48 2 18 120 13 117 3.63e-20 91.7
Msa0177550 AT1G21130.1 31.863 408 207 12 26 423 27 373 1.79e-52 180
Msa0177550 AT1G21100.1 31.863 408 207 11 26 423 27 373 2.12e-52 180
Msa0177550 AT1G77520.1 31.221 426 224 12 6 423 17 381 3.14e-51 177
Msa0177550 AT1G21110.1 31.373 408 209 12 26 423 27 373 2.28e-50 174
Msa0177550 AT1G21120.1 31.127 408 210 12 26 423 27 373 1.36e-49 172
Msa0177550 AT1G21120.2 30.882 408 211 12 26 423 75 421 3.59e-49 172
Msa0177550 AT1G51990.1 44.156 154 81 3 272 422 210 361 1.42e-41 151
Msa0177550 AT1G51990.1 44.242 165 80 4 123 283 206 362 1.46e-41 151
Msa0177550 AT1G51990.1 37.931 116 61 4 12 120 5 116 5.16e-11 64.3
Msa0177550 AT1G51990.2 44.156 154 81 3 272 422 210 361 1.76e-41 150
Msa0177550 AT1G51990.2 44.242 165 80 4 123 283 206 362 1.83e-41 150
Msa0177550 AT1G51990.2 37.931 116 61 4 12 120 5 116 5.07e-11 64.3
Msa0177550 AT1G62900.1 47.297 148 75 3 278 423 59 205 1.20e-40 144
Msa0177550 AT1G62900.1 48.201 139 70 2 132 268 59 197 7.69e-40 142
Msa0177550 AT1G63140.2 47.297 148 75 3 278 423 235 381 6.01e-39 144
Msa0177550 AT1G63140.2 48.201 139 70 2 132 268 235 373 4.12e-38 142
Msa0177550 AT1G63140.4 47.297 148 75 3 278 423 255 401 8.31e-39 144
Msa0177550 AT1G63140.4 48.201 139 70 2 132 268 255 393 5.61e-38 142
Msa0177550 AT5G37170.3 45.223 157 82 4 270 423 31 186 1.89e-37 135
Msa0177550 AT5G37170.3 44.966 149 79 3 123 268 30 178 1.59e-36 132
Msa0177550 AT1G77530.1 48.630 146 72 3 280 423 237 381 8.48e-37 138
Msa0177550 AT1G77530.1 48.905 137 68 2 134 268 237 373 4.52e-35 134
Msa0177550 AT5G37170.2 45.223 157 82 4 270 423 79 234 1.10e-36 134
Msa0177550 AT5G37170.2 44.966 149 79 3 123 268 78 226 1.11e-35 131
Msa0177550 AT1G33030.1 45.570 158 79 4 270 423 196 350 2.67e-36 136
Msa0177550 AT1G33030.1 45.775 142 75 2 123 262 195 336 1.26e-35 134
Msa0177550 AT1G33030.1 42.574 101 54 2 28 125 10 109 6.80e-14 73.2
Msa0177550 AT5G37170.1 45.223 157 82 4 270 423 179 334 8.13e-36 135
Msa0177550 AT5G37170.1 44.966 149 79 3 123 268 178 326 8.54e-35 132
Msa0177550 AT1G76790.1 43.709 151 82 3 275 423 216 365 5.57e-35 133
Msa0177550 AT1G76790.1 43.662 142 78 2 129 268 216 357 2.00e-34 132
Msa0177550 AT5G53810.1 43.243 148 81 3 278 423 232 378 6.53e-34 130
Msa0177550 AT5G53810.1 44.203 138 75 2 132 267 232 369 6.39e-33 128
Msa0177550 AT4G35150.1 42.308 156 79 5 123 269 162 315 1.41e-33 128
Msa0177550 AT4G35150.1 42.308 156 79 5 270 416 163 316 5.09e-33 127
Msa0177550 AT3G53140.1 39.583 144 80 3 123 261 203 344 4.63e-29 117
Msa0177550 AT3G53140.1 37.654 162 93 4 251 407 186 344 1.08e-28 115
Msa0177550 AT4G35160.1 43.571 140 70 4 278 408 228 367 2.40e-28 115
Msa0177550 AT4G35160.1 42.857 140 71 4 132 262 228 367 3.53e-28 115
Msa0177550 AT1G21130.2 30.844 308 145 9 26 325 27 274 2.62e-25 105
Msa0177550 AT1G33030.2 42.574 101 54 2 28 125 10 109 3.22e-14 72.8
Msa0177550 AT1G33030.2 55.172 58 25 1 123 179 195 252 1.13e-11 65.5
Msa0177550 AT1G33030.2 54.386 57 25 1 270 325 196 252 6.83e-11 63.2
Msa0177550 AT1G33030.3 42.574 101 54 2 28 125 10 109 4.20e-14 72.8
Msa0177550 AT1G33030.3 54.098 61 27 1 123 182 195 255 1.13e-12 68.6
Msa0177550 AT1G33030.3 53.333 60 27 1 270 328 196 255 6.69e-12 66.2
Msa0177550 AT1G63140.1 57.143 49 21 0 132 180 255 303 6.08e-12 66.6
Msa0177550 AT1G63140.1 57.143 49 21 0 278 326 255 303 6.08e-12 66.6

Find 88 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTTTATTAATGGGTATT+TGG 0.121685 1_4:-64626456 None:intergenic
TCCCTTTATTAATGGATATT+TGG 0.122440 1_4:-64628646 None:intergenic
AAGTTAATTCCCTTTATTAA+TGG 0.172707 1_4:-64628654 None:intergenic
TCACTTGTGTTTGGTTCTTC+TGG 0.194077 1_4:-64626961 None:intergenic
TCACTTGTGTTTGGTTCTTC+TGG 0.194077 1_4:-64629149 None:intergenic
ACGGCAAGCAATTTGAAATT+TGG 0.215832 1_4:-64627091 None:intergenic
AAGTTAATTCCCTTTATTAA+TGG 0.220033 1_4:-64626464 None:intergenic
GAAAATGCACCACCACTTTC+AGG 0.245970 1_4:+64626506 Msa0177550:CDS
GAAAATGCACCACCACTTTC+AGG 0.245970 1_4:+64628696 Msa0177550:CDS
ACGGCAAGCAACTTGAAATT+TGG 0.263471 1_4:-64629279 None:intergenic
ATCCAAATACCCATTAATAA+AGG 0.266045 1_4:+64626454 Msa0177550:CDS
ATCCAAATATCCATTAATAA+AGG 0.266045 1_4:+64628644 Msa0177550:CDS
AGTTAATTCCCTTTATTAAT+GGG 0.307073 1_4:-64626463 None:intergenic
TGCCGTGCTTTCAACAGTTT+GGG 0.308799 1_4:+64629296 Msa0177550:CDS
TTGCCGTGCTTTCAACAGTT+TGG 0.318040 1_4:+64629295 Msa0177550:CDS
TTAGATTCTTCACTTGTGTT+TGG 0.326373 1_4:-64626970 None:intergenic
TTAGATTCTTCACTTGTGTT+TGG 0.326373 1_4:-64629158 None:intergenic
TGTAGGCTGTATGCCATAAT+TGG 0.348746 1_4:+64629039 Msa0177550:intron
GTTCTGAGGTAGCACTAATT+TGG 0.352301 1_4:-64625400 None:intergenic
AACACTCGGACTTGCCTAAT+AGG 0.352781 1_4:+64625597 Msa0177550:CDS
AACATTCGGTCAAGCCTATT+AGG 0.371607 1_4:-64625611 None:intergenic
GGTGGTGCCGAGAGAGTTTA+CGG 0.386591 1_4:+64625689 Msa0177550:CDS
GTTATCCTCCTTGGTAATGT+AGG 0.401834 1_4:-64625378 None:intergenic
ACTAATTTGGTTATCCTCCT+TGG 0.402910 1_4:-64625387 None:intergenic
TCCAAATACCCATTAATAAA+GGG 0.404369 1_4:+64626455 Msa0177550:CDS
TCCAAATATCCATTAATAAA+GGG 0.404369 1_4:+64628645 Msa0177550:CDS
TGTAGGCTGTGTGCCATAAT+TGG 0.410164 1_4:+64626851 Msa0177550:intron
CACAAAGCTTTATCACCAAA+TGG 0.430052 1_4:+64626910 Msa0177550:CDS
CACAAAGCTTTATCACCAAA+TGG 0.430052 1_4:+64629098 Msa0177550:CDS
TATACTTACCTTCAGTATCA+TGG 0.439779 1_4:-64626703 None:intergenic
TATACTTACCTTCAGTATCA+TGG 0.439779 1_4:-64628895 None:intergenic
AAATAACCTCTACTTTCATC+AGG 0.441916 1_4:-64625740 None:intergenic
GAGAAATTGAGCAAACTCTC+TGG 0.445194 1_4:+64627063 Msa0177550:CDS
GAGAAATTGAGCAAACTCTC+TGG 0.445194 1_4:+64629251 Msa0177550:CDS
GTTGATGTTGGAGGTGGAAG+TGG 0.451719 1_4:+64626410 Msa0177550:intron
GTTGATGTTGGAGGTGGAAG+TGG 0.451719 1_4:+64628600 Msa0177550:intron
ATCCTCGATGGTACGAGTAC+TGG 0.463390 1_4:-64625666 None:intergenic
ATAGCAGCATTTAACACTGC+TGG 0.468445 1_4:-64625479 None:intergenic
CTCATGTTTATCACAGTTGG+TGG 0.480360 1_4:+64627015 Msa0177550:CDS
CTCATGTTTATCACAGTTGG+TGG 0.480360 1_4:+64629203 Msa0177550:CDS
TATGTTTGCAAGTGTTCCAC+AGG 0.500462 1_4:+64626674 Msa0177550:CDS
TATGTTTGCAAGTGTTCCAC+AGG 0.500462 1_4:+64628866 Msa0177550:CDS
GAGAGTTTACGGACTCTCAA+TGG 0.504711 1_4:+64625700 Msa0177550:CDS
TAAGGGATTGAGCATGTTGG+AGG 0.505746 1_4:+64626648 Msa0177550:intron
TAAGGGATTGAGCATGTTGG+AGG 0.505746 1_4:+64628840 Msa0177550:intron
AATCTCATGTTTATCACAGT+TGG 0.506086 1_4:+64627012 Msa0177550:CDS
AATCTCATGTTTATCACAGT+TGG 0.506086 1_4:+64629200 Msa0177550:CDS
TGCTGGATAAACAAGATTAG+TGG 0.509486 1_4:-64625462 None:intergenic
TTTATTTGAGATCATAGCTA+AGG 0.511778 1_4:+64625511 Msa0177550:CDS
TAGCAAGCAAACGCAACATT+CGG 0.513879 1_4:-64625625 None:intergenic
TTTCAACAGTTTGGGAGTGA+TGG 0.524643 1_4:+64629304 Msa0177550:CDS
TGTTTATCACAGTTGGTGGA+AGG 0.529690 1_4:+64627019 Msa0177550:CDS
TGTTTATCACAGTTGGTGGA+AGG 0.529690 1_4:+64629207 Msa0177550:CDS
TCCGAGTGTTGCGTTGATGC+TGG 0.531087 1_4:-64625584 None:intergenic
TTTACGGACTCTCAATGGTC+GGG 0.536710 1_4:+64625705 Msa0177550:CDS
GTTTATCACAGTTGGTGGAA+GGG 0.544261 1_4:+64627020 Msa0177550:CDS
GTTTATCACAGTTGGTGGAA+GGG 0.544261 1_4:+64629208 Msa0177550:CDS
GCCAGCATCAACGCAACACT+CGG 0.555632 1_4:+64625583 Msa0177550:CDS
TGTTAAGGGATTGAGCATGT+TGG 0.556394 1_4:+64626645 Msa0177550:intron
TATCATGTTATACCTGAAAG+TGG 0.563244 1_4:-64626518 None:intergenic
TATCATGTTATACCTGAAAG+TGG 0.563244 1_4:-64628708 None:intergenic
ATAGCTAAGGCAACACCACC+TGG 0.567142 1_4:+64625524 Msa0177550:CDS
CTCCCAAACTGTTGAAAGCA+CGG 0.568203 1_4:-64627110 None:intergenic
CTCCCAAACTGTTGAAAGCA+CGG 0.568203 1_4:-64629298 None:intergenic
TGAGAGTCCGTAAACTCTCT+CGG 0.571590 1_4:-64625696 None:intergenic
CTCGGCACCACCATCCTCGA+TGG 0.574871 1_4:-64625678 None:intergenic
TGTTAAGGGATTGAGCATGT+TGG 0.581677 1_4:+64628837 Msa0177550:intron
GTTTACGGACTCTCAATGGT+CGG 0.585206 1_4:+64625704 Msa0177550:CDS
CTCTACTTTCATCAGGGACA+AGG 0.594593 1_4:-64625733 None:intergenic
ACTGTCTTCAGTTTGTTCTG+AGG 0.597743 1_4:-64625414 None:intergenic
CAGTGCATGTCTTTCAGCAA+TGG 0.602261 1_4:+64625433 Msa0177550:CDS
AGTACTCGTACCATCGAGGA+TGG 0.616377 1_4:+64625668 Msa0177550:CDS
CTTGTCCCTGATGAAAGTAG+AGG 0.618925 1_4:+64625734 Msa0177550:CDS
AAATGGAAAAGTGATTATTG+TGG 0.620942 1_4:+64626927 Msa0177550:CDS
AAATGGAAAAGTGATTATTG+TGG 0.620942 1_4:+64629115 Msa0177550:CDS
GGGAAATTCCTACATTACCA+AGG 0.642464 1_4:+64625370 Msa0177550:CDS
AATAACCTCTACTTTCATCA+GGG 0.648899 1_4:-64625739 None:intergenic
GGGTGATGCCATGATACTGA+AGG 0.650842 1_4:+64626695 Msa0177550:CDS
GGGTGATGCCATGATACTGA+AGG 0.650842 1_4:+64628887 Msa0177550:CDS
ACTCGTACCATCGAGGATGG+TGG 0.675542 1_4:+64625671 Msa0177550:CDS
ATGTTTGCAAGTGTTCCACA+GGG 0.705139 1_4:+64626675 Msa0177550:CDS
ATGTTTGCAAGTGTTCCACA+GGG 0.705139 1_4:+64628867 Msa0177550:CDS
AGTATCATGGCATCACCCTG+TGG 0.705622 1_4:-64626690 None:intergenic
AGTATCATGGCATCACCCTG+TGG 0.705622 1_4:-64628882 None:intergenic
TTCCAGTACTCGTACCATCG+AGG 0.725495 1_4:+64625664 Msa0177550:CDS
AAATTCCTACATTACCAAGG+AGG 0.730361 1_4:+64625373 Msa0177550:CDS
CATGTTATACCTGAAAGTGG+TGG 0.738842 1_4:-64626515 None:intergenic
CATGTTATACCTGAAAGTGG+TGG 0.738842 1_4:-64628705 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTTTTATATTGATGT+TGG + chr1_4:64627460-64627479 Msa0177550:intron 10.0%
!! TCATACATATATAAAATGTA+CGG + chr1_4:64625827-64625846 Msa0177550:intron 15.0%
!! TTTATATATGTATGAGAAAT+AGG - chr1_4:64625822-64625841 None:intergenic 15.0%
!! TCATACATATATAAAATGTA+CGG + chr1_4:64625827-64625846 Msa0177550:intron 15.0%
!!! TTTTTGTAAAAGTTTAAGTT+TGG + chr1_4:64627309-64627328 Msa0177550:intron 15.0%
!! TTAATTTCATATAGTAGAAT+TGG + chr1_4:64627559-64627578 Msa0177550:intron 15.0%
!! TCATACATATATAAAATCAA+GGG + chr1_4:64628167-64628186 Msa0177550:intron 15.0%
!! ATATACACCTATTAAACATA+TGG + chr1_4:64625875-64625894 Msa0177550:intron 20.0%
!! AATGACAAAATATGAATTCA+TGG + chr1_4:64626274-64626293 Msa0177550:intron 20.0%
!! ATGACAAAATATGAATTCAT+GGG + chr1_4:64626275-64626294 Msa0177550:intron 20.0%
!! AGTTAATTCCCTTTATTAAT+GGG - chr1_4:64626466-64626485 None:intergenic 20.0%
!! AAGTTAATTCCCTTTATTAA+TGG - chr1_4:64626467-64626486 None:intergenic 20.0%
!!! TTTGGTTTAATTTGTGATTT+TGG + chr1_4:64627327-64627346 Msa0177550:intron 20.0%
!!! TTGGTTTAATTTGTGATTTT+GGG + chr1_4:64627328-64627347 Msa0177550:intron 20.0%
!! AAACCAAATATATAACGAAA+AGG - chr1_4:64627400-64627419 None:intergenic 20.0%
!! GTTTACAACATTCTATTTAA+TGG - chr1_4:64627593-64627612 None:intergenic 20.0%
!!! GGCTCAATTTTTTTTTAAAT+GGG + chr1_4:64627736-64627755 Msa0177550:intron 20.0%
!!! GCTCAATTTTTTTTTAAATG+GGG + chr1_4:64627737-64627756 Msa0177550:intron 20.0%
!! TGTTAAAATGAAATACTGTT+GGG - chr1_4:64627875-64627894 None:intergenic 20.0%
!! CTCATACATATATAAAATCA+AGG + chr1_4:64628166-64628185 Msa0177550:intron 20.0%
!! ATATTCACCTATTAAACATA+TGG + chr1_4:64628223-64628242 Msa0177550:intron 20.0%
!!! CCATTGATTTGTTAAAAATT+TGG - chr1_4:64628509-64628528 None:intergenic 20.0%
!!! CCAAATTTTTAACAAATCAA+TGG + chr1_4:64628506-64628525 Msa0177550:intron 20.0%
!! ATCCAAATATCCATTAATAA+AGG + chr1_4:64628644-64628663 Msa0177550:CDS 20.0%
!! TCCAAATATCCATTAATAAA+GGG + chr1_4:64628645-64628664 Msa0177550:CDS 20.0%
!! AAGTTAATTCCCTTTATTAA+TGG - chr1_4:64626467-64626486 None:intergenic 20.0%
!! AGTAAAACTTTAGTATAAGA+TGG - chr1_4:64626584-64626603 None:intergenic 20.0%
!! TTTATTTGAGATCATAGCTA+AGG + chr1_4:64625511-64625530 Msa0177550:CDS 25.0%
! TTAAACTATTTATCCGAACA+CGG - chr1_4:64626006-64626025 None:intergenic 25.0%
! ATATTCACCTATGAAACATA+TGG + chr1_4:64626030-64626049 Msa0177550:intron 25.0%
!!! TCTTACTTTTGTTCCAATTA+TGG + chr1_4:64626168-64626187 Msa0177550:intron 25.0%
! AAAATATGAATTCATGGGAA+AGG + chr1_4:64626280-64626299 Msa0177550:intron 25.0%
!!! CCATTGACTTGTTAAATATT+TGG - chr1_4:64626319-64626338 None:intergenic 25.0%
! CCAAATATTTAACAAGTCAA+TGG + chr1_4:64626316-64626335 Msa0177550:intron 25.0%
!! ATATCAACATTAGTTGATGT+TGG + chr1_4:64626398-64626417 Msa0177550:intron 25.0%
! ATCCAAATACCCATTAATAA+AGG + chr1_4:64626454-64626473 Msa0177550:CDS 25.0%
! TCCAAATACCCATTAATAAA+GGG + chr1_4:64626455-64626474 Msa0177550:CDS 25.0%
!! GACTTTGTTTACATAATTGT+AGG + chr1_4:64626834-64626853 Msa0177550:intron 25.0%
!! ATTTTTCATCTGACCAATTA+TGG - chr1_4:64626867-64626886 None:intergenic 25.0%
!!! ACAATAATCACTTTTCCATT+TGG - chr1_4:64626928-64626947 None:intergenic 25.0%
! AAATGGAAAAGTGATTATTG+TGG + chr1_4:64626927-64626946 Msa0177550:CDS 25.0%
! AAATTACATAACATCATAGC+AGG - chr1_4:64627267-64627286 None:intergenic 25.0%
!! AGTCCTTTTCGTTATATATT+TGG + chr1_4:64627394-64627413 Msa0177550:intron 25.0%
!!! GGGCTCAATTTTTTTTTAAA+TGG + chr1_4:64627735-64627754 Msa0177550:intron 25.0%
!! TTTTTAAATGGGGATGTATA+AGG + chr1_4:64627747-64627766 Msa0177550:intron 25.0%
!! TAAGGCAATAATTGGTTTAT+AGG + chr1_4:64627765-64627784 Msa0177550:intron 25.0%
!! AAGGCAATAATTGGTTTATA+GGG + chr1_4:64627766-64627785 Msa0177550:intron 25.0%
! TTGATGTCTAGTAAATGTTT+GGG - chr1_4:64627821-64627840 None:intergenic 25.0%
! ATTGATGTCTAGTAAATGTT+TGG - chr1_4:64627822-64627841 None:intergenic 25.0%
! GTGTTAAAATGAAATACTGT+TGG - chr1_4:64627876-64627895 None:intergenic 25.0%
! AATAACCTTTACTTTCATCA+GGG - chr1_4:64628075-64628094 None:intergenic 25.0%
! AAATAACCTTTACTTTCATC+AGG - chr1_4:64628076-64628095 None:intergenic 25.0%
! TGATGAAAGTAAAGGTTATT+TGG + chr1_4:64628075-64628094 Msa0177550:intron 25.0%
! ATATCCTCCATATGTTTAAT+AGG - chr1_4:64628233-64628252 None:intergenic 25.0%
! GGAGGATATATAAGTTATAA+AGG + chr1_4:64628244-64628263 Msa0177550:intron 25.0%
! TTAAGCTAATTGTAGCAATT+AGG + chr1_4:64626138-64626157 Msa0177550:intron 25.0%
!!! TCTTACTTTTGTTCCAATTA+TGG + chr1_4:64626168-64626187 Msa0177550:intron 25.0%
!! ATATCAACATTAGTTGATGT+TGG + chr1_4:64626398-64626417 Msa0177550:intron 25.0%
!! TCCCTTTATTAATGGATATT+TGG - chr1_4:64628649-64628668 None:intergenic 25.0%
!! GACTTTGTTTACATAATTGT+AGG + chr1_4:64626834-64626853 Msa0177550:intron 25.0%
!! ATTTTTCATCTGACCAATTA+TGG - chr1_4:64626867-64626886 None:intergenic 25.0%
!!! ACAATAATCACTTTTCCATT+TGG - chr1_4:64626928-64626947 None:intergenic 25.0%
! AAATGGAAAAGTGATTATTG+TGG + chr1_4:64626927-64626946 Msa0177550:CDS 25.0%
AATTTAGAAGCGATTTCTGA+TGG - chr1_4:64625563-64625582 None:intergenic 30.0%
AATAACCTCTACTTTCATCA+GGG - chr1_4:64625742-64625761 None:intergenic 30.0%
AAATAACCTCTACTTTCATC+AGG - chr1_4:64625743-64625762 None:intergenic 30.0%
TGATGAAAGTAGAGGTTATT+TGG + chr1_4:64625742-64625761 Msa0177550:intron 30.0%
TACACCTATTAAACATATGG+AGG + chr1_4:64625878-64625897 Msa0177550:intron 30.0%
GTATCCTCCATATGTTTAAT+AGG - chr1_4:64625885-64625904 None:intergenic 30.0%
TTAAACATATGGAGGATACT+TGG + chr1_4:64625886-64625905 Msa0177550:intron 30.0%
TTATCCTCCATATGTTTCAT+AGG - chr1_4:64626040-64626059 None:intergenic 30.0%
ACTAAAGCTTAGACCATAAT+TGG - chr1_4:64626184-64626203 None:intergenic 30.0%
! CTAAGCTTTAGTTTCTTTCA+AGG + chr1_4:64626192-64626211 Msa0177550:intron 30.0%
TCTTTCAAGGATGAACTTTA+AGG + chr1_4:64626205-64626224 Msa0177550:intron 30.0%
AGAATGCTTGAAATATACAC+TGG + chr1_4:64626365-64626384 Msa0177550:intron 30.0%
TGAAATATACACTGGATTTG+AGG + chr1_4:64626373-64626392 Msa0177550:intron 30.0%
GAAATATACACTGGATTTGA+GGG + chr1_4:64626374-64626393 Msa0177550:intron 30.0%
! TCCCTTTATTAATGGGTATT+TGG - chr1_4:64626459-64626478 None:intergenic 30.0%
! TGGTGCATTTTCAATAACTT+GGG - chr1_4:64626498-64626517 None:intergenic 30.0%
TATCATGTTATACCTGAAAG+TGG - chr1_4:64626521-64626540 None:intergenic 30.0%
TATACTACCAACTCTTGTTA+AGG + chr1_4:64626630-64626649 Msa0177550:intron 30.0%
ATACTACCAACTCTTGTTAA+GGG + chr1_4:64626631-64626650 Msa0177550:intron 30.0%
TATACTTACCTTCAGTATCA+TGG - chr1_4:64626706-64626725 None:intergenic 30.0%
TTAGATTCTTCACTTGTGTT+TGG - chr1_4:64626973-64626992 None:intergenic 30.0%
AATCTCATGTTTATCACAGT+TGG + chr1_4:64627012-64627031 Msa0177550:CDS 30.0%
AGTGATTACAACAATAGCTT+TGG + chr1_4:64627153-64627172 Msa0177550:intron 30.0%
TCAATGTGTTAAGAGTAAAG+TGG + chr1_4:64627185-64627204 Msa0177550:intron 30.0%
CAATGTGTTAAGAGTAAAGT+GGG + chr1_4:64627186-64627205 Msa0177550:intron 30.0%
CAAAAAATAGCAAATAGCGT+CGG + chr1_4:64627691-64627710 Msa0177550:intron 30.0%
! AGGCAATAATTGGTTTATAG+GGG + chr1_4:64627767-64627786 Msa0177550:intron 30.0%
ATATAGCACTAAGCGTAAAA+AGG + chr1_4:64627794-64627813 Msa0177550:intron 30.0%
TATAGCACTAAGCGTAAAAA+GGG + chr1_4:64627795-64627814 Msa0177550:intron 30.0%
! CTGTTGGGTTGTAAAAAAAA+GGG - chr1_4:64627860-64627879 None:intergenic 30.0%
! ACTGTTGGGTTGTAAAAAAA+AGG - chr1_4:64627861-64627880 None:intergenic 30.0%
!! TGAAAATGAAAGTGTTGTGT+CGG - chr1_4:64627901-64627920 None:intergenic 30.0%
AATATTTACTCGAACCATTG+AGG + chr1_4:64627997-64628016 Msa0177550:intron 30.0%
TTCACCTATTAAACATATGG+AGG + chr1_4:64628226-64628245 Msa0177550:intron 30.0%
ACTAAAGCTTAGACCATAAT+TGG - chr1_4:64626184-64626203 None:intergenic 30.0%
! TCTATCCAGGATGAATTTTA+AGG + chr1_4:64628395-64628414 Msa0177550:intron 30.0%
AGTGACAAAGTATGAATTCA+TGG + chr1_4:64628464-64628483 Msa0177550:intron 30.0%
GTGACAAAGTATGAATTCAT+GGG + chr1_4:64628465-64628484 Msa0177550:intron 30.0%
AAAGTATGAATTCATGGGAA+AGG + chr1_4:64628470-64628489 Msa0177550:intron 30.0%
AGAATGCTTGAAATATACAC+TGG + chr1_4:64626365-64626384 Msa0177550:intron 30.0%
TGAAATATACACTGGATTTG+AGG + chr1_4:64626373-64626392 Msa0177550:intron 30.0%
GAAATATACACTGGATTTGA+GGG + chr1_4:64626374-64626393 Msa0177550:intron 30.0%
! TGGTGCATTTTCAATAACTT+GGG - chr1_4:64626498-64626517 None:intergenic 30.0%
TATCATGTTATACCTGAAAG+TGG - chr1_4:64626521-64626540 None:intergenic 30.0%
! TATACCACCAACTTTTGTTA+AGG + chr1_4:64628822-64628841 Msa0177550:intron 30.0%
! ATACCACCAACTTTTGTTAA+GGG + chr1_4:64628823-64628842 Msa0177550:intron 30.0%
TATACTTACCTTCAGTATCA+TGG - chr1_4:64626706-64626725 None:intergenic 30.0%
TTAGATTCTTCACTTGTGTT+TGG - chr1_4:64626973-64626992 None:intergenic 30.0%
AATCTCATGTTTATCACAGT+TGG + chr1_4:64627012-64627031 Msa0177550:CDS 30.0%
AAATTCCTACATTACCAAGG+AGG + chr1_4:64625373-64625392 Msa0177550:CDS 35.0%
ACTAATTTGGTTATCCTCCT+TGG - chr1_4:64625390-64625409 None:intergenic 35.0%
TGCTGGATAAACAAGATTAG+TGG - chr1_4:64625465-64625484 None:intergenic 35.0%
ATCCTGCATTGTTACAAGTT+TGG + chr1_4:64625789-64625808 Msa0177550:intron 35.0%
TACCAAACTTGTAACAATGC+AGG - chr1_4:64625794-64625813 None:intergenic 35.0%
AAATGTACGGATAGACAAAG+TGG + chr1_4:64625840-64625859 Msa0177550:intron 35.0%
TGCAGGATAACATAGAAATG+TGG - chr1_4:64625777-64625796 None:intergenic 35.0%
!! CCAAACTTGTTAACAAATGC+AGG - chr1_4:64625938-64625957 None:intergenic 35.0%
! CCTGCATTTGTTAACAAGTT+TGG + chr1_4:64625935-64625954 Msa0177550:intron 35.0%
TTCACCTATGAAACATATGG+AGG + chr1_4:64626033-64626052 Msa0177550:intron 35.0%
! GATTTGTTCAAGAACGTTCA+TGG + chr1_4:64626251-64626270 Msa0177550:intron 35.0%
! TCAACATTAGTTGATGTTGG+AGG + chr1_4:64626401-64626420 Msa0177550:intron 35.0%
! GGTGCATTTTCAATAACTTG+GGG - chr1_4:64626497-64626516 None:intergenic 35.0%
! GTGGTGCATTTTCAATAACT+TGG - chr1_4:64626499-64626518 None:intergenic 35.0%
! CACAAAGCTTTATCACCAAA+TGG + chr1_4:64626910-64626929 Msa0177550:CDS 35.0%
! ACGGCAAGCAATTTGAAATT+TGG - chr1_4:64627094-64627113 None:intergenic 35.0%
AGAGTAAAGTGGGAGAATAA+AGG + chr1_4:64627196-64627215 Msa0177550:intron 35.0%
! AGAATAAAGGCCTTTTTGTG+AGG + chr1_4:64627209-64627228 Msa0177550:intron 35.0%
AACAACATGACCTCACAAAA+AGG - chr1_4:64627222-64627241 None:intergenic 35.0%
GTCACGAAATATTGTTAGTG+CGG + chr1_4:64627370-64627389 Msa0177550:intron 35.0%
!!! CGACGCTATTTGCTATTTTT+TGG - chr1_4:64627693-64627712 None:intergenic 35.0%
GGGATGTATAAGGCAATAAT+TGG + chr1_4:64627757-64627776 Msa0177550:intron 35.0%
! GGCAATAATTGGTTTATAGG+GGG + chr1_4:64627768-64627787 Msa0177550:intron 35.0%
AAAAAGGGTGAAACGACTAA+TGG - chr1_4:64627845-64627864 None:intergenic 35.0%
!! AAAATGAAAGTGTTGTGTCG+GGG - chr1_4:64627899-64627918 None:intergenic 35.0%
!! GAAAATGAAAGTGTTGTGTC+GGG - chr1_4:64627900-64627919 None:intergenic 35.0%
ATCCTGCATTGTTACAAGTT+TGG + chr1_4:64625789-64625808 Msa0177550:intron 35.0%
TACCAAACTTGTAACAATGC+AGG - chr1_4:64625794-64625813 None:intergenic 35.0%
TCAAGGGATATATAGTGACT+TGG + chr1_4:64628183-64628202 Msa0177550:intron 35.0%
! CTAAGCTTTAGTTTCTATCC+AGG + chr1_4:64628382-64628401 Msa0177550:intron 35.0%
!! CAGGATGAATTTTAAGGAAG+CGG + chr1_4:64628401-64628420 Msa0177550:intron 35.0%
! GATTTGTTCAAGAACGTTCA+TGG + chr1_4:64626251-64626270 Msa0177550:intron 35.0%
! TCAACATTAGTTGATGTTGG+AGG + chr1_4:64626401-64626420 Msa0177550:intron 35.0%
! GGTGCATTTTCAATAACTTG+GGG - chr1_4:64626497-64626516 None:intergenic 35.0%
! GTGGTGCATTTTCAATAACT+TGG - chr1_4:64626499-64626518 None:intergenic 35.0%
AATCCCTTAACAAAAGTTGG+TGG - chr1_4:64628829-64628848 None:intergenic 35.0%
CTCAATCCCTTAACAAAAGT+TGG - chr1_4:64628832-64628851 None:intergenic 35.0%
! CACAAAGCTTTATCACCAAA+TGG + chr1_4:64626910-64626929 Msa0177550:CDS 35.0%
GGGAAATTCCTACATTACCA+AGG + chr1_4:64625370-64625389 Msa0177550:CDS 40.0%
!! GTTATCCTCCTTGGTAATGT+AGG - chr1_4:64625381-64625400 None:intergenic 40.0%
!! GTTCTGAGGTAGCACTAATT+TGG - chr1_4:64625403-64625422 None:intergenic 40.0%
! ACTGTCTTCAGTTTGTTCTG+AGG - chr1_4:64625417-64625436 None:intergenic 40.0%
ATAGCAGCATTTAACACTGC+TGG - chr1_4:64625482-64625501 None:intergenic 40.0%
AACATTCGGTCAAGCCTATT+AGG - chr1_4:64625614-64625633 None:intergenic 40.0%
TAGCAAGCAAACGCAACATT+CGG - chr1_4:64625628-64625647 None:intergenic 40.0%
! GAACTTTAAGGAAGCAGTAG+TGG + chr1_4:64626217-64626236 Msa0177550:intron 40.0%
! ACATTAGTTGATGTTGGAGG+TGG + chr1_4:64626404-64626423 Msa0177550:intron 40.0%
CATGTTATACCTGAAAGTGG+TGG - chr1_4:64626518-64626537 None:intergenic 40.0%
CTCAATCCCTTAACAAGAGT+TGG - chr1_4:64626640-64626659 None:intergenic 40.0%
TGTTAAGGGATTGAGCATGT+TGG + chr1_4:64626645-64626664 Msa0177550:intron 40.0%
TATGTTTGCAAGTGTTCCAC+AGG + chr1_4:64626674-64626693 Msa0177550:CDS 40.0%
ATGTTTGCAAGTGTTCCACA+GGG + chr1_4:64626675-64626694 Msa0177550:CDS 40.0%
!! TCACTTGTGTTTGGTTCTTC+TGG - chr1_4:64626964-64626983 None:intergenic 40.0%
CTCATGTTTATCACAGTTGG+TGG + chr1_4:64627015-64627034 Msa0177550:CDS 40.0%
!! TGTTTATCACAGTTGGTGGA+AGG + chr1_4:64627019-64627038 Msa0177550:CDS 40.0%
!! GTTTATCACAGTTGGTGGAA+GGG + chr1_4:64627020-64627039 Msa0177550:CDS 40.0%
GAGAAATTGAGCAAACTCTC+TGG + chr1_4:64627063-64627082 Msa0177550:CDS 40.0%
TTTCAACAGTTTGGGAGTGA+TGG + chr1_4:64627116-64627135 Msa0177550:intron 40.0%
!! AAATGAAAGTGTTGTGTCGG+GGG - chr1_4:64627898-64627917 None:intergenic 40.0%
TTTCGCCTAATGAAATGTGG+GGG - chr1_4:64627925-64627944 None:intergenic 40.0%
GTTTCGCCTAATGAAATGTG+GGG - chr1_4:64627926-64627945 None:intergenic 40.0%
CGTTTCGCCTAATGAAATGT+GGG - chr1_4:64627927-64627946 None:intergenic 40.0%
TTTACTCGAACCATTGAGGA+TGG + chr1_4:64628001-64628020 Msa0177550:intron 40.0%
CTTTACTTTCATCAGGGACA+AGG - chr1_4:64628069-64628088 None:intergenic 40.0%
CTTGTCCCTGATGAAAGTAA+AGG + chr1_4:64628067-64628086 Msa0177550:intron 40.0%
CGCTTCCTTAAAATTCATCC+TGG - chr1_4:64628403-64628422 None:intergenic 40.0%
!! GATGAATTTTAAGGAAGCGG+TGG + chr1_4:64628404-64628423 Msa0177550:intron 40.0%
! ACATTAGTTGATGTTGGAGG+TGG + chr1_4:64626404-64626423 Msa0177550:intron 40.0%
CATGTTATACCTGAAAGTGG+TGG - chr1_4:64626518-64626537 None:intergenic 40.0%
TGTTAAGGGATTGAGCATGT+TGG + chr1_4:64626645-64626664 Msa0177550:intron 40.0%
TATGTTTGCAAGTGTTCCAC+AGG + chr1_4:64626674-64626693 Msa0177550:CDS 40.0%
ATGTTTGCAAGTGTTCCACA+GGG + chr1_4:64626675-64626694 Msa0177550:CDS 40.0%
TGTAGGCTGTATGCCATAAT+TGG + chr1_4:64629039-64629058 Msa0177550:intron 40.0%
!! TCACTTGTGTTTGGTTCTTC+TGG - chr1_4:64626964-64626983 None:intergenic 40.0%
CTCATGTTTATCACAGTTGG+TGG + chr1_4:64627015-64627034 Msa0177550:CDS 40.0%
!! TGTTTATCACAGTTGGTGGA+AGG + chr1_4:64627019-64627038 Msa0177550:CDS 40.0%
!! GTTTATCACAGTTGGTGGAA+GGG + chr1_4:64627020-64627039 Msa0177550:CDS 40.0%
GAGAAATTGAGCAAACTCTC+TGG + chr1_4:64627063-64627082 Msa0177550:CDS 40.0%
ACGGCAAGCAACTTGAAATT+TGG - chr1_4:64629282-64629301 None:intergenic 40.0%
TTTCAACAGTTTGGGAGTGA+TGG + chr1_4:64627116-64627135 Msa0177550:intron 40.0%
CAGTGCATGTCTTTCAGCAA+TGG + chr1_4:64625433-64625452 Msa0177550:CDS 45.0%
AACACTCGGACTTGCCTAAT+AGG + chr1_4:64625597-64625616 Msa0177550:CDS 45.0%
TGAGAGTCCGTAAACTCTCT+CGG - chr1_4:64625699-64625718 None:intergenic 45.0%
GAGAGTTTACGGACTCTCAA+TGG + chr1_4:64625700-64625719 Msa0177550:CDS 45.0%
GTTTACGGACTCTCAATGGT+CGG + chr1_4:64625704-64625723 Msa0177550:CDS 45.0%
TTTACGGACTCTCAATGGTC+GGG + chr1_4:64625705-64625724 Msa0177550:CDS 45.0%
CTCTACTTTCATCAGGGACA+AGG - chr1_4:64625736-64625755 None:intergenic 45.0%
CTTGTCCCTGATGAAAGTAG+AGG + chr1_4:64625734-64625753 Msa0177550:CDS 45.0%
GAAAATGCACCACCACTTTC+AGG + chr1_4:64626506-64626525 Msa0177550:CDS 45.0%
TAAGGGATTGAGCATGTTGG+AGG + chr1_4:64626648-64626667 Msa0177550:intron 45.0%
TGTAGGCTGTGTGCCATAAT+TGG + chr1_4:64626851-64626870 Msa0177550:intron 45.0%
TTGCCGTGCTTTCAACAGTT+TGG + chr1_4:64627107-64627126 Msa0177550:intron 45.0%
TGCCGTGCTTTCAACAGTTT+GGG + chr1_4:64627108-64627127 Msa0177550:intron 45.0%
CTCCCAAACTGTTGAAAGCA+CGG - chr1_4:64627113-64627132 None:intergenic 45.0%
TTCACCCCCCACATTTCATT+AGG + chr1_4:64627917-64627936 Msa0177550:intron 45.0%
TTCGCCTAATGAAATGTGGG+GGG - chr1_4:64627924-64627943 None:intergenic 45.0%
GCGTTTCGCCTAATGAAATG+TGG - chr1_4:64627928-64627947 None:intergenic 45.0%
TGAGAGTCCGTAAACTCTCT+CGG - chr1_4:64625699-64625718 None:intergenic 45.0%
GAGAGTTTACGGACTCTCAA+TGG + chr1_4:64625700-64625719 Msa0177550:CDS 45.0%
GTTTACGGACTCTCAATGGT+CGG + chr1_4:64625704-64625723 Msa0177550:CDS 45.0%
!! GAATTTTAAGGAAGCGGTGG+TGG + chr1_4:64628407-64628426 Msa0177550:intron 45.0%
GAAAATGCACCACCACTTTC+AGG + chr1_4:64626506-64626525 Msa0177550:CDS 45.0%
TAAGGGATTGAGCATGTTGG+AGG + chr1_4:64626648-64626667 Msa0177550:intron 45.0%
TTGCCGTGCTTTCAACAGTT+TGG + chr1_4:64627107-64627126 Msa0177550:intron 45.0%
TGCCGTGCTTTCAACAGTTT+GGG + chr1_4:64627108-64627127 Msa0177550:intron 45.0%
CTCCCAAACTGTTGAAAGCA+CGG - chr1_4:64627113-64627132 None:intergenic 45.0%
ATAGCTAAGGCAACACCACC+TGG + chr1_4:64625524-64625543 Msa0177550:CDS 50.0%
!! GATGGTGACATGAAAGCACC+AGG - chr1_4:64625545-64625564 None:intergenic 50.0%
TTCCAGTACTCGTACCATCG+AGG + chr1_4:64625664-64625683 Msa0177550:CDS 50.0%
ATCCTCGATGGTACGAGTAC+TGG - chr1_4:64625669-64625688 None:intergenic 50.0%
AGTACTCGTACCATCGAGGA+TGG + chr1_4:64625668-64625687 Msa0177550:CDS 50.0%
GTACGGATAGTGACCGTGTT+CGG + chr1_4:64625990-64626009 Msa0177550:intron 50.0%
GTTGATGTTGGAGGTGGAAG+TGG + chr1_4:64626410-64626429 Msa0177550:intron 50.0%
AGTATCATGGCATCACCCTG+TGG - chr1_4:64626693-64626712 None:intergenic 50.0%
GGGTGATGCCATGATACTGA+AGG + chr1_4:64626695-64626714 Msa0177550:CDS 50.0%
!! ATAGCAAATAGCGTCGGCCT+AGG + chr1_4:64627697-64627716 Msa0177550:intron 50.0%
ACTCGAACCATTGAGGATGG+TGG + chr1_4:64628004-64628023 Msa0177550:intron 50.0%
GTTGATGTTGGAGGTGGAAG+TGG + chr1_4:64626410-64626429 Msa0177550:intron 50.0%
AGTATCATGGCATCACCCTG+TGG - chr1_4:64626693-64626712 None:intergenic 50.0%
GGGTGATGCCATGATACTGA+AGG + chr1_4:64626695-64626714 Msa0177550:CDS 50.0%
!! GGTGACATGAAAGCACCAGG+TGG - chr1_4:64625542-64625561 None:intergenic 55.0%
GCCAGCATCAACGCAACACT+CGG + chr1_4:64625583-64625602 Msa0177550:CDS 55.0%
! TCCGAGTGTTGCGTTGATGC+TGG - chr1_4:64625587-64625606 None:intergenic 55.0%
ACTCGTACCATCGAGGATGG+TGG + chr1_4:64625671-64625690 Msa0177550:CDS 55.0%
! GGTGGTGCCGAGAGAGTTTA+CGG + chr1_4:64625689-64625708 Msa0177550:CDS 55.0%
GCCTAGGATCCATGTCAGCA+GGG + chr1_4:64627713-64627732 Msa0177550:intron 55.0%
CCTAGGATCCATGTCAGCAG+GGG + chr1_4:64627714-64627733 Msa0177550:intron 55.0%
CTAGGATCCATGTCAGCAGG+GGG + chr1_4:64627715-64627734 Msa0177550:intron 55.0%
AATTGAGCCCCCTGCTGACA+TGG - chr1_4:64627725-64627744 None:intergenic 55.0%
! GGTGGTGCCGAGAGAGTTTA+CGG + chr1_4:64625689-64625708 Msa0177550:CDS 55.0%
GGCCTAGGATCCATGTCAGC+AGG + chr1_4:64627712-64627731 Msa0177550:intron 60.0%
CCCCTGCTGACATGGATCCT+AGG - chr1_4:64627717-64627736 None:intergenic 60.0%
CTCGGCACCACCATCCTCAA+TGG - chr1_4:64628014-64628033 None:intergenic 60.0%
CTCGGCACCACCATCCTCGA+TGG - chr1_4:64625681-64625700 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 64625368 64629340 64625368 ID=Msa0177550;Name=Msa0177550
chr1_4 mRNA 64625368 64629340 64625368 ID=Msa0177550-mRNA-1;Parent=Msa0177550;Name=Msa0177550-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0|0|1|0.57|0.37|8|0|423
chr1_4 exon 64625368 64625545 64625368 ID=Msa0177550-mRNA-1:exon:20990;Parent=Msa0177550-mRNA-1
chr1_4 exon 64625567 64625755 64625567 ID=Msa0177550-mRNA-1:exon:20991;Parent=Msa0177550-mRNA-1
chr1_4 exon 64626423 64626527 64626423 ID=Msa0177550-mRNA-1:exon:20992;Parent=Msa0177550-mRNA-1
chr1_4 exon 64626652 64626716 64626652 ID=Msa0177550-mRNA-1:exon:20993;Parent=Msa0177550-mRNA-1
chr1_4 exon 64626856 64627123 64626856 ID=Msa0177550-mRNA-1:exon:20994;Parent=Msa0177550-mRNA-1
chr1_4 exon 64628613 64628717 64628613 ID=Msa0177550-mRNA-1:exon:20995;Parent=Msa0177550-mRNA-1
chr1_4 exon 64628844 64628908 64628844 ID=Msa0177550-mRNA-1:exon:20996;Parent=Msa0177550-mRNA-1
chr1_4 exon 64629044 64629340 64629044 ID=Msa0177550-mRNA-1:exon:20997;Parent=Msa0177550-mRNA-1
chr1_4 CDS 64625368 64625545 64625368 ID=Msa0177550-mRNA-1:cds;Parent=Msa0177550-mRNA-1
chr1_4 CDS 64625567 64625755 64625567 ID=Msa0177550-mRNA-1:cds;Parent=Msa0177550-mRNA-1
chr1_4 CDS 64626423 64626527 64626423 ID=Msa0177550-mRNA-1:cds;Parent=Msa0177550-mRNA-1
chr1_4 CDS 64626652 64626716 64626652 ID=Msa0177550-mRNA-1:cds;Parent=Msa0177550-mRNA-1
chr1_4 CDS 64626856 64627123 64626856 ID=Msa0177550-mRNA-1:cds;Parent=Msa0177550-mRNA-1
chr1_4 CDS 64628613 64628717 64628613 ID=Msa0177550-mRNA-1:cds;Parent=Msa0177550-mRNA-1
chr1_4 CDS 64628844 64628908 64628844 ID=Msa0177550-mRNA-1:cds;Parent=Msa0177550-mRNA-1
chr1_4 CDS 64629044 64629340 64629044 ID=Msa0177550-mRNA-1:cds;Parent=Msa0177550-mRNA-1
Gene Sequence

>Msa0177550

ATGGGAAATTCCTACATTACCAAGGAGGATAACCAAATTAGTGCTACCTCAGAACAAACTGAAGACAGTGCATGTCTTTCAGCAATGGTACTTACCACTAATCTTGTTTATCCAGCAGTGTTAAATGCTGCTATTGATCTCAATTTATTTGAGATCATAGCTAAGGCAACACCACCTGAAATCGCTTCTAAATTGCCAGCATCAACGCAACACTCGGACTTGCCTAATAGGCTTGACCGAATGTTGCGTTTGCTTGCTAGTTATTCTGTTCTTACTTCCAGTACTCGTACCATCGAGGATGGTGGTGCCGAGAGAGTTTACGGACTCTCAATGGTCGGGAAATACCTTGTCCCTGATGAAAGTAGAGGTGGAAGTGGAAGAAATCTTGAATTGATAATATCCAAATACCCATTAATAAAGGGAATTAACTTTGATCTGCCCCAAGTTATTGAAAATGCACCACCACTTTCAGGGATTGAGCATGTTGGAGGAGATATGTTTGCAAGTGTTCCACAGGGTGATGCCATGATACTGAAGGCTGTGTGCCATAATTGGTCAGATGAAAAATGCATTGAATTTTTGAGCAATTGTCACAAAGCTTTATCACCAAATGGAAAAGTGATTATTGTGGAGTTCATATTGCCAGAAGAACCAAACACAAGTGAAGAATCTAAGCTTGTTTCAACTCTTGACAATCTCATGTTTATCACAGTTGGTGGAAGGGAAAGAACTGAGAAACAATATGAGAAATTGAGCAAACTCTCTGGATTTTCCAAATTTCAAATTGCTTGCCGTGCTTTCAACAGTGGAAGTGGAAGAAATCTTGAATTGATAATATCCAAATATCCATTAATAAAGGGAATTAACTTTGATCTTCCCCAAGTTATTGAAAATGCACCACCACTTTCAGGGATTGAGCATGTTGGAGGAGATATGTTTGCAAGTGTTCCACAGGGTGATGCCATGATACTGAAGGCTGTATGCCATAATTGGTCAGATGAAAAATGCATAGAATTTTTAAGCAATTGTCACAAAGCTTTATCACCAAATGGAAAAGTGATTATTGTGGAGTTCATATTGCCAGAAGAACCAAACACAAGTGAAGAATCTAAGCTTGTTTCAACTCTTGACAATCTCATGTTTATCACAGTTGGTGGAAGGGAAAGAACTGAGAAACAATATGAGAAATTGAGCAAACTCTCTGGATTTTCCAAATTTCAAGTTGCTTGCCGTGCTTTCAACAGTTTGGGAGTGATGGAATTTTATAAATGA
Protein sequence

>Msa0177550

MGNSYITKEDNQISATSEQTEDSACLSAMVLTTNLVYPAVLNAAIDLNLFEIIAKATPPEIASKLPASTQHSDLPNRLDRMLRLLASYSVLTSSTRTIEDGGAERVYGLSMVGKYLVPDESRGGSGRNLELIISKYPLIKGINFDLPQVIENAPPLSGIEHVGGDMFASVPQGDAMILKAVCHNWSDEKCIEFLSNCHKALSPNGKVIIVEFILPEEPNTSEESKLVSTLDNLMFITVGGRERTEKQYEKLSKLSGFSKFQIACRAFNSGSGRNLELIISKYPLIKGINFDLPQVIENAPPLSGIEHVGGDMFASVPQGDAMILKAVCHNWSDEKCIEFLSNCHKALSPNGKVIIVEFILPEEPNTSEESKLVSTLDNLMFITVGGRERTEKQYEKLSKLSGFSKFQVACRAFNSLGVMEFYK*