Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0349910 | RHN65810.1 | 93.506 | 77 | 5 | 0 | 1 | 77 | 1 | 77 | 1.31e-45 | 151 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0349910 | sp|P51074|ANX4_FRAAN | 69.863 | 73 | 22 | 0 | 1 | 73 | 1 | 73 | 7.32e-30 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0349910 | A0A396IKM9 | 93.506 | 77 | 5 | 0 | 1 | 77 | 1 | 77 | 6.25e-46 | 151 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004340 | Msa0349910 | -0.801871 | 7.340063e-49 | -8.615850e-47 |
| Msa0004580 | Msa0349910 | 0.809136 | 2.196535e-50 | -8.615850e-47 |
| Msa0004590 | Msa0349910 | 0.814818 | 1.269168e-51 | -8.615850e-47 |
| Msa0004940 | Msa0349910 | 0.810385 | 1.183367e-50 | -8.615850e-47 |
| Msa0004990 | Msa0349910 | 0.804048 | 2.604648e-49 | -8.615850e-47 |
| Msa0006080 | Msa0349910 | 0.809261 | 2.064973e-50 | -8.615850e-47 |
| Msa0010150 | Msa0349910 | 0.849508 | 3.092374e-60 | -8.615850e-47 |
| Msa0011010 | Msa0349910 | 0.832951 | 7.049560e-56 | -8.615850e-47 |
| Msa0011040 | Msa0349910 | 0.835397 | 1.718455e-56 | -8.615850e-47 |
| Msa0011060 | Msa0349910 | -0.814821 | 1.267288e-51 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0349910 | MtrunA17_Chr3g0084011 | 93.506 | 77 | 5 | 0 | 1 | 77 | 1 | 77 | 1.20e-49 | 151 |
| Msa0349910 | MtrunA17_Chr3g0083921 | 98.630 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 5.20e-47 | 151 |
| Msa0349910 | MtrunA17_Chr8g0352591 | 76.712 | 73 | 17 | 0 | 1 | 73 | 1 | 73 | 5.46e-35 | 120 |
| Msa0349910 | MtrunA17_Chr3g0083901 | 72.603 | 73 | 20 | 0 | 1 | 73 | 1 | 73 | 3.60e-32 | 113 |
| Msa0349910 | MtrunA17_Chr8g0352611 | 69.863 | 73 | 22 | 0 | 1 | 73 | 1 | 73 | 7.68e-32 | 112 |
| Msa0349910 | MtrunA17_Chr8g0352621 | 69.863 | 73 | 21 | 1 | 1 | 73 | 1 | 72 | 2.60e-31 | 110 |
| Msa0349910 | MtrunA17_Chr2g0293441 | 56.164 | 73 | 32 | 0 | 1 | 73 | 1 | 73 | 1.26e-24 | 93.2 |
| Msa0349910 | MtrunA17_Chr8g0382281 | 55.405 | 74 | 32 | 1 | 1 | 73 | 1 | 74 | 3.80e-20 | 81.3 |
| Msa0349910 | MtrunA17_Chr5g0425971 | 52.703 | 74 | 34 | 1 | 1 | 73 | 1 | 74 | 8.24e-20 | 80.5 |
| Msa0349910 | MtrunA17_Chr8g0393281 | 54.054 | 74 | 33 | 1 | 1 | 73 | 1 | 74 | 1.90e-19 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0349910 | AT5G12380.1 | 61.644 | 73 | 28 | 0 | 1 | 73 | 1 | 73 | 4.55e-27 | 100 |
| Msa0349910 | AT5G10220.1 | 58.108 | 74 | 30 | 1 | 1 | 73 | 1 | 74 | 1.57e-21 | 85.5 |
| Msa0349910 | AT5G65020.1 | 58.108 | 74 | 30 | 1 | 1 | 73 | 1 | 74 | 1.72e-21 | 85.5 |
| Msa0349910 | AT5G10230.1 | 58.108 | 74 | 30 | 1 | 1 | 73 | 1 | 74 | 2.00e-21 | 85.1 |
| Msa0349910 | AT2G38760.1 | 52.055 | 73 | 34 | 1 | 1 | 72 | 1 | 73 | 5.95e-21 | 84.0 |
| Msa0349910 | AT1G35720.1 | 55.405 | 74 | 32 | 1 | 1 | 73 | 1 | 74 | 3.17e-20 | 82.0 |
| Msa0349910 | AT5G65020.2 | 61.224 | 49 | 19 | 0 | 25 | 73 | 11 | 59 | 9.87e-15 | 67.0 |
| Msa0349910 | AT1G68090.2 | 50.704 | 71 | 34 | 1 | 1 | 70 | 1 | 71 | 9.38e-14 | 63.9 |
| Msa0349910 | AT1G68090.1 | 50.704 | 71 | 34 | 1 | 1 | 70 | 1 | 71 | 1.39e-13 | 63.9 |
Find 17 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCCATTATAGCAATATT+AGG | 0.311002 | 3_1:-14317118 | Msa0349910:CDS |
| AAGATGCAGAAGCTCTTCAA+AGG | 0.524239 | 3_1:-14317854 | Msa0349910:CDS |
| AGAGCTTCTGCATCTTCAAC+TGG | 0.416203 | 3_1:+14317861 | None:intergenic |
| AGATGCAGAAGCTCTTCAAA+GGG | 0.589809 | 3_1:-14317853 | Msa0349910:CDS |
| AGGAGCAATAAGGGAAGCCA+TGG | 0.568515 | 3_1:+14317896 | None:intergenic |
| AGTGGTTGGAAGGAGCAATA+AGG | 0.292571 | 3_1:+14317886 | None:intergenic |
| ATACTATGAACTCTATCAAG+AGG | 0.590523 | 3_1:-14317056 | Msa0349910:CDS |
| ATCTTCAACTGGAGAGTGGT+TGG | 0.525012 | 3_1:+14317872 | None:intergenic |
| ATGACCTAATATTGCTATAA+TGG | 0.265808 | 3_1:+14317114 | None:intergenic |
| CGTCTTGAGTCTGAGCTTTC+AGG | 0.157675 | 3_1:-14317022 | Msa0349910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACGATTCCATGTGTTATAAT+CGG | - | chr3_1:14317638-14317657 | Msa0349910:intron | 30.0% | |
| ATACTATGAACTCTATCAAG+AGG | - | chr3_1:14317835-14317854 | Msa0349910:intron | 30.0% | |
| ACGATTCCATGTGTTATAAT+CGG | - | chr3_1:14317638-14317657 | Msa0349910:intron | 30.0% | |
| ATACTATGAACTCTATCAAG+AGG | - | chr3_1:14317835-14317854 | Msa0349910:intron | 30.0% | |
| TTTAGTCCTTTACCTTCGAT+GGG | + | chr3_1:14317417-14317436 | None:intergenic | 35.0% | |
| ATCGAAGGTAAAGGACTAAA+GGG | - | chr3_1:14317417-14317436 | Msa0349910:intron | 35.0% | |
| AATATTGGCACCAGGAAAAA+GGG | + | chr3_1:14317472-14317491 | None:intergenic | 35.0% | |
| GGTGTAACATGTCAGAATAT+TGG | + | chr3_1:14317487-14317506 | None:intergenic | 35.0% | |
| TTTAGTCCTTTACCTTCGAT+GGG | + | chr3_1:14317417-14317436 | None:intergenic | 35.0% | |
| ATCGAAGGTAAAGGACTAAA+GGG | - | chr3_1:14317417-14317436 | Msa0349910:intron | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 14316997 | 14317916 | 14316997 | ID=Msa0349910;Name=Msa0349910 |
| chr3_1 | mRNA | 14316997 | 14317916 | 14316997 | ID=Msa0349910-mRNA-1;Parent=Msa0349910;Name=Msa0349910-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|1|1|2|0|77 |
| chr3_1 | exon | 14317844 | 14317916 | 14317844 | ID=Msa0349910-mRNA-1:exon:3571;Parent=Msa0349910-mRNA-1 |
| chr3_1 | exon | 14316997 | 14317157 | 14316997 | ID=Msa0349910-mRNA-1:exon:3570;Parent=Msa0349910-mRNA-1 |
| chr3_1 | CDS | 14317844 | 14317916 | 14317844 | ID=Msa0349910-mRNA-1:cds;Parent=Msa0349910-mRNA-1 |
| chr3_1 | CDS | 14316997 | 14317157 | 14316997 | ID=Msa0349910-mRNA-1:cds;Parent=Msa0349910-mRNA-1 |
| Gene Sequence |
>Msa0349910 ATGGCTTCCCTTATTGCTCCTTCCAACCACTCTCCAGTTGAAGATGCAGAAGCTCTTCAAAGGGCTGTTAAAGGTTGGGGAGCTGATGAAAAAGCCATTATAGCAATATTAGGTCATAGAAATGCTACCCAAAGGACTCAAATCAGACAAGCATACTATGAACTCTATCAAGAGGATCTCATCAAACGTCTTGAGTCTGAGCTTTCAGGTGATTTTGAGGTACATCCACGGTAA |
| Protein sequence |
>Msa0349910 MASLIAPSNHSPVEDAEALQRAVKGWGADEKAIIAILGHRNATQRTQIRQAYYELYQEDLIKRLESELSGDFEVHPR* |
