Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018682.t1 | XP_013449911.1 | 100 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 3.70E-29 | 136.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018682.t1 | Q93XX8 | 79.7 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 2.6e-23 | 108.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018682.t1 | A0A072U2M3 | 100.0 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 2.7e-29 | 136.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene046040 | MS.gene018682 | PPI |
| MS.gene018682 | MS.gene033017 | PPI |
| MS.gene018682 | MS.gene067809 | PPI |
| MS.gene064180 | MS.gene018682 | PPI |
| MS.gene45344 | MS.gene018682 | PPI |
| MS.gene018682 | MS.gene52097 | PPI |
| MS.gene98436 | MS.gene018682 | PPI |
| MS.gene056257 | MS.gene018682 | PPI |
| MS.gene95758 | MS.gene018682 | PPI |
| MS.gene059422 | MS.gene018682 | PPI |
| MS.gene08205 | MS.gene018682 | PPI |
| MS.gene018682 | MS.gene001986 | PPI |
| MS.gene057730 | MS.gene018682 | PPI |
| MS.gene90954 | MS.gene018682 | PPI |
| MS.gene41916 | MS.gene018682 | PPI |
| MS.gene31515 | MS.gene018682 | PPI |
| MS.gene72894 | MS.gene018682 | PPI |
| MS.gene037324 | MS.gene018682 | PPI |
| MS.gene51868 | MS.gene018682 | PPI |
| MS.gene90957 | MS.gene018682 | PPI |
| MS.gene018682 | MS.gene044498 | PPI |
| MS.gene000714 | MS.gene018682 | PPI |
| MS.gene003501 | MS.gene018682 | PPI |
| MS.gene99744 | MS.gene018682 | PPI |
| MS.gene029242 | MS.gene018682 | PPI |
| MS.gene018682 | MS.gene054186 | PPI |
| MS.gene028023 | MS.gene018682 | PPI |
| MS.gene68979 | MS.gene018682 | PPI |
| MS.gene038800 | MS.gene018682 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018682.t1 | MTR_7g097110 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 2.26e-43 | 134 |
| MS.gene018682.t1 | MTR_8g069770 | 85.938 | 64 | 9 | 0 | 1 | 64 | 1 | 64 | 7.70e-36 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018682.t1 | AT2G20490 | 79.688 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 8.91e-33 | 107 |
| MS.gene018682.t1 | AT2G20490 | 79.688 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 8.91e-33 | 107 |
| MS.gene018682.t1 | AT2G20490 | 78.125 | 64 | 13 | 1 | 1 | 64 | 1 | 63 | 2.46e-30 | 101 |
Find 17 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATTCAAGGTTTACCTTAG+TGG | 0.314345 | 7.1:-10666215 | None:intergenic |
| AATACTTTGGAGCTGGATGC+TGG | 0.333958 | 7.1:-10667872 | None:intergenic |
| GTTAAACTGAAGAAGCGATT+TGG | 0.348976 | 7.1:+10667837 | MS.gene018682:CDS |
| TGCTCTGAAAATTTGTCATC+AGG | 0.402146 | 7.1:-10667810 | None:intergenic |
| ATTTGTCATCAGGTGAAAAC+CGG | 0.402459 | 7.1:-10667800 | None:intergenic |
| TTGAATATAGCTCAATACTT+TGG | 0.457998 | 7.1:-10667885 | None:intergenic |
| TGCAGAAAGAGTCACCGCTT+GGG | 0.506313 | 7.1:+10666336 | MS.gene018682:intron |
| ATAGCTCAATACTTTGGAGC+TGG | 0.511390 | 7.1:-10667879 | None:intergenic |
| TTGCAGAAAGAGTCACCGCT+TGG | 0.526675 | 7.1:+10666335 | MS.gene018682:intron |
| GCTACTCAATCTGCCCATCC+AGG | 0.527175 | 7.1:+10666362 | MS.gene018682:CDS |
| TTTGGAGCTGGATGCTGGGT+CGG | 0.555768 | 7.1:-10667867 | None:intergenic |
| ATACTTTGGAGCTGGATGCT+GGG | 0.560513 | 7.1:-10667871 | None:intergenic |
| TTTGTCATCAGGTGAAAACC+GGG | 0.561589 | 7.1:-10667799 | None:intergenic |
| CATTAACGATAACGGTGACA+AGG | 0.605553 | 7.1:+10666186 | MS.gene018682:CDS |
| AATGTAAAACTGAAGATACA+TGG | 0.612653 | 7.1:-10666167 | None:intergenic |
| TGACAAGGTCTACACCACTA+AGG | 0.622254 | 7.1:+10666201 | MS.gene018682:CDS |
| GATTGAGTAGCTAACCCAAG+CGG | 0.725473 | 7.1:-10666350 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAGTTTTCAAAACAAAAA+AGG | - | chr7.1:10667692-10667711 | None:intergenic | 15.0% |
| !!! | AAAGTTTTCAAAACAAAAAA+GGG | - | chr7.1:10667691-10667710 | None:intergenic | 15.0% |
| !!! | TTTTAACATTCACTTAAAAT+TGG | - | chr7.1:10666774-10666793 | None:intergenic | 15.0% |
| !! | AAAAAGTCATTAATCATTGT+AGG | - | chr7.1:10667757-10667776 | None:intergenic | 20.0% |
| !! | AACAACAAAAGAATTAAATG+AGG | - | chr7.1:10667461-10667480 | None:intergenic | 20.0% |
| !! | ACAACAAAAGAATTAAATGA+GGG | - | chr7.1:10667460-10667479 | None:intergenic | 20.0% |
| !! | ATAAGAAGTCTATGTTAAAA+TGG | + | chr7.1:10666684-10666703 | MS.gene018682:intron | 20.0% |
| !! | TAAAGAAAAATGACTTGATT+TGG | + | chr7.1:10667487-10667506 | MS.gene018682:intron | 20.0% |
| !! | TAAGAAGTCTATGTTAAAAT+GGG | + | chr7.1:10666685-10666704 | MS.gene018682:intron | 20.0% |
| !!! | CAATTTTTTACCTCAAAAAT+TGG | - | chr7.1:10666590-10666609 | None:intergenic | 20.0% |
| ! | AAGAAGTCTATGTTAAAATG+GGG | + | chr7.1:10666686-10666705 | MS.gene018682:intron | 25.0% |
| ! | CATTAATCATTGTAGGAAAT+TGG | - | chr7.1:10667750-10667769 | None:intergenic | 25.0% |
| ! | GGTAAAAAATTGAAACTGAT+AGG | + | chr7.1:10666598-10666617 | MS.gene018682:intron | 25.0% |
| ! | GTATTAAAATCCTTGATTCA+AGG | - | chr7.1:10666231-10666250 | None:intergenic | 25.0% |
| ! | TATGTGTCATGTCAAAATTT+AGG | + | chr7.1:10666893-10666912 | MS.gene018682:intron | 25.0% |
| ! | TCAAAGATTAAAGCACAAAA+TGG | - | chr7.1:10666418-10666437 | None:intergenic | 25.0% |
| !! | CAGTTTTACATTAACGATAA+CGG | + | chr7.1:10666178-10666197 | MS.gene018682:CDS | 25.0% |
| !!! | AAATGACTTGATTTGGAATT+TGG | + | chr7.1:10667494-10667513 | MS.gene018682:intron | 25.0% |
| !!! | ATGTTTTGAATTGTGAAGTA+AGG | + | chr7.1:10667578-10667597 | MS.gene018682:intron | 25.0% |
| !!! | CCTAAGTTAAATTGTTTTAG+CGG | - | chr7.1:10667397-10667416 | None:intergenic | 25.0% |
| !!! | TGTTTTGAATTGTGAAGTAA+GGG | + | chr7.1:10667579-10667598 | MS.gene018682:intron | 25.0% |
| AAAAATTGGATGTGTGTCAT+CGG | - | chr7.1:10666576-10666595 | None:intergenic | 30.0% | |
| AAAACATTTGCATCACACTT+TGG | - | chr7.1:10667565-10667584 | None:intergenic | 30.0% | |
| AACTACAATAAGTCGTTGAT+TGG | - | chr7.1:10667206-10667225 | None:intergenic | 30.0% | |
| ACAAAATGGCAGTAAAACTA+TGG | - | chr7.1:10666404-10666423 | None:intergenic | 30.0% | |
| ACACTCTGATACATAAGTAT+TGG | - | chr7.1:10667258-10667277 | None:intergenic | 30.0% | |
| AGAAACTTTGGATTTGCAAT+TGG | - | chr7.1:10667129-10667148 | None:intergenic | 30.0% | |
| AGTGGATCATGAAAAAGAAT+AGG | - | chr7.1:10666920-10666939 | None:intergenic | 30.0% | |
| CAATTCGTATTTACTGCAAT+TGG | + | chr7.1:10666728-10666747 | MS.gene018682:intron | 30.0% | |
| CTAAAGAGATACGTTAAACA+CGG | - | chr7.1:10667004-10667023 | None:intergenic | 30.0% | |
| GTATTTACTGCAATTGGATT+TGG | + | chr7.1:10666734-10666753 | MS.gene018682:intron | 30.0% | |
| TTAATAGCAAAGAAAGAGAG+AGG | - | chr7.1:10667533-10667552 | None:intergenic | 30.0% | |
| TTGGAATTTGGATTTGCAAT+AGG | - | chr7.1:10667110-10667129 | None:intergenic | 30.0% | |
| TTGGATTTGCAATTGGAATT+TGG | - | chr7.1:10667122-10667141 | None:intergenic | 30.0% | |
| TTGGATTTGCAATTGGTTAT+AGG | + | chr7.1:10666640-10666659 | MS.gene018682:intron | 30.0% | |
| TTTGCAATGAGAATTAAGAG+TGG | - | chr7.1:10666963-10666982 | None:intergenic | 30.0% | |
| ! | CCGCTAAAACAATTTAACTT+AGG | + | chr7.1:10667394-10667413 | MS.gene018682:intron | 30.0% |
| ! | TCGTGGGTTGTAAATAATAT+TGG | - | chr7.1:10667726-10667745 | None:intergenic | 30.0% |
| ! | TTTTAGCAAAATACCTGGAT+GGG | - | chr7.1:10666378-10666397 | None:intergenic | 30.0% |
| !! | ATTTTAGCAAAATACCTGGA+TGG | - | chr7.1:10666379-10666398 | None:intergenic | 30.0% |
| !! | GTGCTTTAATCTTTGAAACA+TGG | + | chr7.1:10666422-10666441 | MS.gene018682:intron | 30.0% |
| !! | GTGGTTTTGCAATTAGAATT+GGG | + | chr7.1:10666837-10666856 | MS.gene018682:intron | 30.0% |
| !!! | GTGATTTTGGTTACAACATT+AGG | + | chr7.1:10667333-10667352 | MS.gene018682:intron | 30.0% |
| AAAGGGCAGTGTTAAATTAG+TGG | - | chr7.1:10667674-10667693 | None:intergenic | 35.0% | |
| AAGTCTATGTTAAAATGGGG+AGG | + | chr7.1:10666689-10666708 | MS.gene018682:intron | 35.0% | |
| ACTGCAATTGGATTTGGATT+TGG | + | chr7.1:10666740-10666759 | MS.gene018682:intron | 35.0% | |
| AGGCAAAGTAAGCAAATTGA+TGG | - | chr7.1:10667160-10667179 | None:intergenic | 35.0% | |
| ATTGTAGGAAATTGGATCGT+GGG | - | chr7.1:10667742-10667761 | None:intergenic | 35.0% | |
| ATTTGTCATCAGGTGAAAAC+CGG | - | chr7.1:10667803-10667822 | None:intergenic | 35.0% | |
| CTAAGGTAAACCTTGAATCA+AGG | + | chr7.1:10666218-10666237 | MS.gene018682:intron | 35.0% | |
| GGATTTGCAATAGGAAACAA+GGG | - | chr7.1:10667101-10667120 | None:intergenic | 35.0% | |
| GGCAAAGTAAGCAAATTGAT+GGG | - | chr7.1:10667159-10667178 | None:intergenic | 35.0% | |
| GTTAAACTGAAGAAGCGATT+TGG | + | chr7.1:10667837-10667856 | MS.gene018682:CDS | 35.0% | |
| GTTGGATTTGGATTTGCAAT+TGG | + | chr7.1:10666633-10666652 | MS.gene018682:intron | 35.0% | |
| TGATTCAAGGTTTACCTTAG+TGG | - | chr7.1:10666218-10666237 | None:intergenic | 35.0% | |
| TGGATTTGCAATAGGAAACA+AGG | - | chr7.1:10667102-10667121 | None:intergenic | 35.0% | |
| TGTAACCAAAATCACTGTGT+GGG | - | chr7.1:10667328-10667347 | None:intergenic | 35.0% | |
| TTGTAACCAAAATCACTGTG+TGG | - | chr7.1:10667329-10667348 | None:intergenic | 35.0% | |
| TTTGCAATTAGAATTGGGAG+TGG | + | chr7.1:10666842-10666861 | MS.gene018682:intron | 35.0% | |
| TTTGCAATTGGTTATAGGAG+AGG | + | chr7.1:10666645-10666664 | MS.gene018682:intron | 35.0% | |
| ! | ATCTGATGCATTTTCCGTAT+TGG | + | chr7.1:10667422-10667441 | MS.gene018682:intron | 35.0% |
| ! | TCAATTTGCTTACTTTGCCT+TGG | + | chr7.1:10667160-10667179 | MS.gene018682:intron | 35.0% |
| ! | TCTGATGCATTTTCCGTATT+GGG | + | chr7.1:10667423-10667442 | MS.gene018682:intron | 35.0% |
| ! | TGACACACATCCAATTTTTG+AGG | + | chr7.1:10666577-10666596 | MS.gene018682:intron | 35.0% |
| !! | CGTGGTTTTGCAATTAGAAT+TGG | + | chr7.1:10666836-10666855 | MS.gene018682:intron | 35.0% |
| !! | GGTGATTTTAGCAAAATACC+TGG | - | chr7.1:10666383-10666402 | None:intergenic | 35.0% |
| !! | TGCTCTGAAAATTTGTCATC+AGG | - | chr7.1:10667813-10667832 | None:intergenic | 35.0% |
| AATAGGAAACAAGGGATGGT+AGG | - | chr7.1:10667093-10667112 | None:intergenic | 40.0% | |
| AGACAAAGAGACTTCACACA+TGG | + | chr7.1:10666808-10666827 | MS.gene018682:intron | 40.0% | |
| ATGGGTCGTGATAGAAACTT+TGG | - | chr7.1:10667141-10667160 | None:intergenic | 40.0% | |
| CATTGTAGGAAATTGGATCG+TGG | - | chr7.1:10667743-10667762 | None:intergenic | 40.0% | |
| GCAATGAGAATTAAGAGTGG+CGG | - | chr7.1:10666960-10666979 | None:intergenic | 40.0% | |
| GCACATTTGAGAATTGAGAG+TGG | - | chr7.1:10666938-10666957 | None:intergenic | 40.0% | |
| GTAACCAAAATCACTGTGTG+GGG | - | chr7.1:10667327-10667346 | None:intergenic | 40.0% | |
| TAACCAAAATCACTGTGTGG+GGG | - | chr7.1:10667326-10667345 | None:intergenic | 40.0% | |
| TCAGCTAGAAACGTTGGATT+TGG | + | chr7.1:10666621-10666640 | MS.gene018682:intron | 40.0% | |
| TGTTAAAATGGGGAGGCAAT+TGG | + | chr7.1:10666696-10666715 | MS.gene018682:intron | 40.0% | |
| TTGCAATAGGAAACAAGGGA+TGG | - | chr7.1:10667097-10667116 | None:intergenic | 40.0% | |
| TTTGTCATCAGGTGAAAACC+GGG | - | chr7.1:10667802-10667821 | None:intergenic | 40.0% | |
| ! | AAGCATGGACACATGAGTTT+TGG | - | chr7.1:10667291-10667310 | None:intergenic | 40.0% |
| ! | CATTAACGATAACGGTGACA+AGG | + | chr7.1:10666186-10666205 | MS.gene018682:CDS | 40.0% |
| ! | CTGATGCATTTTCCGTATTG+GGG | + | chr7.1:10667424-10667443 | MS.gene018682:intron | 40.0% |
| !! | GCACTCTTTTGATTCTGCAT+TGG | + | chr7.1:10667637-10667656 | MS.gene018682:intron | 40.0% |
| AAATCACTGTGTGGGGGAAA+AGG | - | chr7.1:10667320-10667339 | None:intergenic | 45.0% | |
| AAGAGACTTCACACATGGAG+AGG | + | chr7.1:10666813-10666832 | MS.gene018682:intron | 45.0% | |
| GAGATACGTTAAACACGGAG+AGG | - | chr7.1:10666999-10667018 | None:intergenic | 45.0% | |
| GATAGGTCAGCTAGAAACGT+TGG | + | chr7.1:10666615-10666634 | MS.gene018682:intron | 45.0% | |
| GATTGAGTAGCTAACCCAAG+CGG | - | chr7.1:10666353-10666372 | None:intergenic | 45.0% | |
| GCAATTAGAATTGGGAGTGG+CGG | + | chr7.1:10666845-10666864 | MS.gene018682:intron | 45.0% | |
| TCAACACACGTCAATCTCCA+AGG | - | chr7.1:10667180-10667199 | None:intergenic | 45.0% | |
| TGACAAGGTCTACACCACTA+AGG | + | chr7.1:10666201-10666220 | MS.gene018682:CDS | 45.0% | |
| TGGAGATTGACGTGTGTTGA+TGG | + | chr7.1:10667180-10667199 | MS.gene018682:intron | 45.0% | |
| ! | TTTCCCCCACACAGTGATTT+TGG | + | chr7.1:10667320-10667339 | MS.gene018682:intron | 45.0% |
| !! | AATACTTTGGAGCTGGATGC+TGG | - | chr7.1:10667875-10667894 | None:intergenic | 45.0% |
| !! | ATACTTTGGAGCTGGATGCT+GGG | - | chr7.1:10667874-10667893 | None:intergenic | 45.0% |
| !! | GGGAAAAGGTCTTTGAAGCA+TGG | - | chr7.1:10667306-10667325 | None:intergenic | 45.0% |
| !!! | ATAATTATTTTTTTTAAAGA+AGG | + | chr7.1:10667229-10667248 | MS.gene018682:intron | 5.0% |
| GGTCTAGAGAAGCCCCAATA+CGG | - | chr7.1:10667439-10667458 | None:intergenic | 50.0% | |
| TGAAAACCGGGCTGCAGTTT+CGG | - | chr7.1:10667790-10667809 | None:intergenic | 50.0% | |
| TGCAGAAAGAGTCACCGCTT+GGG | + | chr7.1:10666336-10666355 | MS.gene018682:intron | 50.0% | |
| TTGCAGAAAGAGTCACCGCT+TGG | + | chr7.1:10666335-10666354 | MS.gene018682:intron | 50.0% | |
| ! | TGTTTTCCGAAACTGCAGCC+CGG | + | chr7.1:10667781-10667800 | MS.gene018682:intron | 50.0% |
| ACTTCACACATGGAGAGGCG+TGG | + | chr7.1:10666818-10666837 | MS.gene018682:intron | 55.0% | |
| GCTACTCAATCTGCCCATCC+AGG | + | chr7.1:10666362-10666381 | MS.gene018682:CDS | 55.0% | |
| ! | TTTGGAGCTGGATGCTGGGT+CGG | - | chr7.1:10667870-10667889 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 10666169 | 10667896 | 10666169 | ID=MS.gene018682 |
| chr7.1 | mRNA | 10666169 | 10667896 | 10666169 | ID=MS.gene018682.t1;Parent=MS.gene018682 |
| chr7.1 | exon | 10666169 | 10666222 | 10666169 | ID=MS.gene018682.t1.exon1;Parent=MS.gene018682.t1 |
| chr7.1 | CDS | 10666169 | 10666222 | 10666169 | ID=cds.MS.gene018682.t1;Parent=MS.gene018682.t1 |
| chr7.1 | exon | 10666341 | 10666383 | 10666341 | ID=MS.gene018682.t1.exon2;Parent=MS.gene018682.t1 |
| chr7.1 | CDS | 10666341 | 10666383 | 10666341 | ID=cds.MS.gene018682.t1;Parent=MS.gene018682.t1 |
| chr7.1 | exon | 10667799 | 10667896 | 10667799 | ID=MS.gene018682.t1.exon3;Parent=MS.gene018682.t1 |
| chr7.1 | CDS | 10667799 | 10667896 | 10667799 | ID=cds.MS.gene018682.t1;Parent=MS.gene018682.t1 |
| Gene Sequence |
| Protein sequence |
