Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020385.t1 | XP_013449911.1 | 100 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 3.70E-29 | 136.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020385.t1 | Q93XX8 | 79.7 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 2.6e-23 | 108.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020385.t1 | A0A072U2M3 | 100.0 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 2.7e-29 | 136.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene020385 | MS.gene029242 | PPI |
| MS.gene020385 | MS.gene08205 | PPI |
| MS.gene020385 | MS.gene90954 | PPI |
| MS.gene020385 | MS.gene064180 | PPI |
| MS.gene020385 | MS.gene31515 | PPI |
| MS.gene020385 | MS.gene038800 | PPI |
| MS.gene020385 | MS.gene41916 | PPI |
| MS.gene020385 | MS.gene45344 | PPI |
| MS.gene020385 | MS.gene001986 | PPI |
| MS.gene020385 | MS.gene003501 | PPI |
| MS.gene020385 | MS.gene000714 | PPI |
| MS.gene020385 | MS.gene51868 | PPI |
| MS.gene020385 | MS.gene68979 | PPI |
| MS.gene020385 | MS.gene054186 | PPI |
| MS.gene020385 | MS.gene056257 | PPI |
| MS.gene020385 | MS.gene72894 | PPI |
| MS.gene020385 | MS.gene044498 | PPI |
| MS.gene020385 | MS.gene90957 | PPI |
| MS.gene020385 | MS.gene52097 | PPI |
| MS.gene020385 | MS.gene037324 | PPI |
| MS.gene020385 | MS.gene057730 | PPI |
| MS.gene020385 | MS.gene033017 | PPI |
| MS.gene020385 | MS.gene067809 | PPI |
| MS.gene020385 | MS.gene028023 | PPI |
| MS.gene020385 | MS.gene95758 | PPI |
| MS.gene020385 | MS.gene98436 | PPI |
| MS.gene020385 | MS.gene046040 | PPI |
| MS.gene020385 | MS.gene059422 | PPI |
| MS.gene020385 | MS.gene99744 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020385.t1 | MTR_7g097110 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 2.26e-43 | 134 |
| MS.gene020385.t1 | MTR_8g069770 | 85.938 | 64 | 9 | 0 | 1 | 64 | 1 | 64 | 7.70e-36 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020385.t1 | AT2G20490 | 79.688 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 8.91e-33 | 107 |
| MS.gene020385.t1 | AT2G20490 | 79.688 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 8.91e-33 | 107 |
| MS.gene020385.t1 | AT2G20490 | 78.125 | 64 | 13 | 1 | 1 | 64 | 1 | 63 | 2.46e-30 | 101 |
Find 17 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATTCAAGGTTTACCTTAG+TGG | 0.314345 | 7.2:-13254515 | None:intergenic |
| AATACTTTGGAGCTGGATGC+TGG | 0.320451 | 7.2:-13256050 | None:intergenic |
| GTTAAACTGAAGAAGCGATT+TGG | 0.348976 | 7.2:+13256015 | MS.gene020385:CDS |
| TGCTCTGAAAATTTGTCATC+AGG | 0.402146 | 7.2:-13255988 | None:intergenic |
| ATTTGTCATCAGGTGAAAAC+CGG | 0.402459 | 7.2:-13255978 | None:intergenic |
| TTGAATATAGCTCAATACTT+TGG | 0.457998 | 7.2:-13256063 | None:intergenic |
| TGCAGAAAGAGTCACCGCTT+GGG | 0.506313 | 7.2:+13254609 | MS.gene020385:intron |
| TTTGGAGCTGGATGCTGGGT+TGG | 0.510089 | 7.2:-13256045 | None:intergenic |
| ATACTTTGGAGCTGGATGCT+GGG | 0.511217 | 7.2:-13256049 | None:intergenic |
| ATAGCTCAATACTTTGGAGC+TGG | 0.511390 | 7.2:-13256057 | None:intergenic |
| TTGCAGAAAGAGTCACCGCT+TGG | 0.526675 | 7.2:+13254608 | MS.gene020385:intron |
| GCTACTCAATCTGCTCATCC+AGG | 0.527880 | 7.2:+13254635 | MS.gene020385:CDS |
| TTTGTCATCAGGTGAAAACC+GGG | 0.561589 | 7.2:-13255977 | None:intergenic |
| GATGTAAAACTGAAGATACA+TGG | 0.619474 | 7.2:-13254467 | None:intergenic |
| TGACAAGGTCTACACCACTA+AGG | 0.622254 | 7.2:+13254501 | MS.gene020385:CDS |
| CATCAACGATAACGGTGACA+AGG | 0.625395 | 7.2:+13254486 | MS.gene020385:CDS |
| GATTGAGTAGCTAACCCAAG+CGG | 0.699776 | 7.2:-13254623 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATATATATATTTAAAA+AGG | + | chr7.2:13255394-13255413 | MS.gene020385:intron | 0.0% |
| !!! | AATAAAGAAATATTACTTTT+TGG | + | chr7.2:13255670-13255689 | MS.gene020385:intron | 10.0% |
| !! | TAAAAAAAAAATGAGACATA+GGG | + | chr7.2:13255732-13255751 | MS.gene020385:intron | 15.0% |
| !! | TCAAAGATTAAAGAAAAAAA+TGG | - | chr7.2:13254691-13254710 | None:intergenic | 15.0% |
| !!! | AAATATTACTTTTTGGAATT+TGG | + | chr7.2:13255677-13255696 | MS.gene020385:intron | 15.0% |
| !!! | TATATTTTTCAAATAAACGT+TGG | - | chr7.2:13254559-13254578 | None:intergenic | 15.0% |
| !!! | TTTTGCATTCTAAATAATAT+TGG | - | chr7.2:13255903-13255922 | None:intergenic | 15.0% |
| !! | CTAAAAAAAAAATGAGACAT+AGG | + | chr7.2:13255731-13255750 | MS.gene020385:intron | 20.0% |
| !! | TAAAGTAAGGAAATTGATAT+GGG | - | chr7.2:13255320-13255339 | None:intergenic | 20.0% |
| !! | TATTGAAAATTGAAACTGAT+AGG | + | chr7.2:13254879-13254898 | MS.gene020385:intron | 20.0% |
| !!! | CTCAAGTTAAATTGTTTTAA+CGG | - | chr7.2:13255581-13255600 | None:intergenic | 20.0% |
| !!! | TTTCTTTAATCTTTGAAACA+TGG | + | chr7.2:13254695-13254714 | MS.gene020385:intron | 20.0% |
| ! | AAGTAAAAAATGAGAACTAG+AGG | - | chr7.2:13255205-13255224 | None:intergenic | 25.0% |
| ! | AGTAAAAAATGAGAACTAGA+GGG | - | chr7.2:13255204-13255223 | None:intergenic | 25.0% |
| ! | CATTAATAGTTGTAGAAAGT+TGG | - | chr7.2:13255927-13255946 | None:intergenic | 25.0% |
| ! | GTAAAGTAAGGAAATTGATA+TGG | - | chr7.2:13255321-13255340 | None:intergenic | 25.0% |
| ! | GTATTAAAATCCTTGATTCA+AGG | - | chr7.2:13254531-13254550 | None:intergenic | 25.0% |
| ! | TAGAAGTTGTCAAAACAAAA+AGG | - | chr7.2:13255870-13255889 | None:intergenic | 25.0% |
| ! | TCTATGTGTCATGAAAATTT+AGG | + | chr7.2:13255077-13255096 | MS.gene020385:intron | 25.0% |
| !! | CAATATTTTACCTCGAAAAT+TGG | - | chr7.2:13254864-13254883 | None:intergenic | 25.0% |
| !!! | ATGTTTTGAATTGTGAAGTA+AGG | + | chr7.2:13255758-13255777 | MS.gene020385:intron | 25.0% |
| !!! | CGTTAAAACAATTTAACTTG+AGG | + | chr7.2:13255579-13255598 | MS.gene020385:intron | 25.0% |
| !!! | GATATTTGTTGTCAAAGTAT+TGG | + | chr7.2:13254771-13254790 | MS.gene020385:intron | 25.0% |
| !!! | TGTTTTGAATTGTGAAGTAA+GGG | + | chr7.2:13255759-13255778 | MS.gene020385:intron | 25.0% |
| TCAATTTCCTTACTTTACCT+TGG | + | chr7.2:13255323-13255342 | MS.gene020385:intron | 30.0% | |
| TGGATTTGCAATTGGATATA+GGG | + | chr7.2:13254922-13254941 | MS.gene020385:intron | 30.0% | |
| TTGGATTTGCAATTGGATAT+AGG | + | chr7.2:13254921-13254940 | MS.gene020385:intron | 30.0% | |
| TTTGCAATGAGAATTAAGAG+TGG | - | chr7.2:13255147-13255166 | None:intergenic | 30.0% | |
| TTTGCATTTGGATTTGCAAT+TGG | + | chr7.2:13254914-13254933 | MS.gene020385:intron | 30.0% | |
| ! | CAGTTTTACATCAACGATAA+CGG | + | chr7.2:13254478-13254497 | MS.gene020385:CDS | 30.0% |
| ! | TTAGGACCTATTCATTTTCA+TGG | + | chr7.2:13255095-13255114 | MS.gene020385:intron | 30.0% |
| !! | GCTAATTTTAGCAAAATACC+TGG | - | chr7.2:13254656-13254675 | None:intergenic | 30.0% |
| !!! | GTGATTTTGGTTACAACATT+AGG | + | chr7.2:13255516-13255535 | MS.gene020385:intron | 30.0% |
| AACAACAAAAGAAGTAGACG+AGG | - | chr7.2:13255646-13255665 | None:intergenic | 35.0% | |
| AATCGAAAACAAGGGATGAT+AGG | - | chr7.2:13255273-13255292 | None:intergenic | 35.0% | |
| ACAACAAAAGAAGTAGACGA+GGG | - | chr7.2:13255645-13255664 | None:intergenic | 35.0% | |
| AGACAGACACATTTGAGAAT+AGG | + | chr7.2:13255047-13255066 | MS.gene020385:intron | 35.0% | |
| AGAGAGATTCAAACATAGAG+AGG | + | chr7.2:13255002-13255021 | MS.gene020385:intron | 35.0% | |
| AGTGGACCATGAAAATGAAT+AGG | - | chr7.2:13255104-13255123 | None:intergenic | 35.0% | |
| ATTTGTCATCAGGTGAAAAC+CGG | - | chr7.2:13255981-13256000 | None:intergenic | 35.0% | |
| CTAAGGTAAACCTTGAATCA+AGG | + | chr7.2:13254518-13254537 | MS.gene020385:intron | 35.0% | |
| GGATTTGCAATCGAAAACAA+GGG | - | chr7.2:13255281-13255300 | None:intergenic | 35.0% | |
| GGATTTGCAATTGGATATAG+GGG | + | chr7.2:13254923-13254942 | MS.gene020385:intron | 35.0% | |
| GTTAAACTGAAGAAGCGATT+TGG | + | chr7.2:13256015-13256034 | MS.gene020385:CDS | 35.0% | |
| GTTGTAACCAAAATCACTGT+GGG | - | chr7.2:13255513-13255532 | None:intergenic | 35.0% | |
| TCAATCTCCAAGGTAAAGTA+AGG | - | chr7.2:13255333-13255352 | None:intergenic | 35.0% | |
| TCAGCTAGAAACTTTGCATT+TGG | + | chr7.2:13254902-13254921 | MS.gene020385:intron | 35.0% | |
| TGATTCAAGGTTTACCTTAG+TGG | - | chr7.2:13254518-13254537 | None:intergenic | 35.0% | |
| TGGATTTGCAATCGAAAACA+AGG | - | chr7.2:13255282-13255301 | None:intergenic | 35.0% | |
| TGTTGTAACCAAAATCACTG+TGG | - | chr7.2:13255514-13255533 | None:intergenic | 35.0% | |
| TTGTAACCAAAATCACTGTG+GGG | - | chr7.2:13255512-13255531 | None:intergenic | 35.0% | |
| ! | TAGGGGAGGTATACATAAAA+AGG | + | chr7.2:13254940-13254959 | MS.gene020385:intron | 35.0% |
| !! | TGCTCTGAAAATTTGTCATC+AGG | - | chr7.2:13255991-13256010 | None:intergenic | 35.0% |
| AAGCATGGACACATGAGTTT+CGG | - | chr7.2:13255477-13255496 | None:intergenic | 40.0% | |
| ATGGGTCGTGTTAGAAACTT+TGG | - | chr7.2:13255302-13255321 | None:intergenic | 40.0% | |
| GAAAATTGGATGCGTGTCAT+TGG | - | chr7.2:13254850-13254869 | None:intergenic | 40.0% | |
| GATTCAAACATAGAGAGGTG+TGG | + | chr7.2:13255007-13255026 | MS.gene020385:intron | 40.0% | |
| GCAATGAGAATTAAGAGTGG+CGG | - | chr7.2:13255144-13255163 | None:intergenic | 40.0% | |
| GCACATTTGAGAATTGAGAG+TGG | - | chr7.2:13255122-13255141 | None:intergenic | 40.0% | |
| TTTGCAATTGGATATAGGGG+AGG | + | chr7.2:13254926-13254945 | MS.gene020385:intron | 40.0% | |
| TTTGTCATCAGGTGAAAACC+GGG | - | chr7.2:13255980-13255999 | None:intergenic | 40.0% | |
| ! | ATCTGATGCATTTTCCGTGT+TGG | + | chr7.2:13255607-13255626 | MS.gene020385:intron | 40.0% |
| ! | TCTGATGCATTTTCCGTGTT+GGG | + | chr7.2:13255608-13255627 | MS.gene020385:intron | 40.0% |
| !! | GCACTCTTTTGATTCTGCAT+TGG | + | chr7.2:13255816-13255835 | MS.gene020385:intron | 40.0% |
| CCAAAATCACTGTGGGGAAA+AGG | - | chr7.2:13255506-13255525 | None:intergenic | 45.0% | |
| CTAGAGGGATACGTTGAACA+TGG | - | chr7.2:13255189-13255208 | None:intergenic | 45.0% | |
| GATTGAGTAGCTAACCCAAG+CGG | - | chr7.2:13254626-13254645 | None:intergenic | 45.0% | |
| GGTTTATAGAAGCCCCAACA+CGG | - | chr7.2:13255624-13255643 | None:intergenic | 45.0% | |
| TGACAAGGTCTACACCACTA+AGG | + | chr7.2:13254501-13254520 | MS.gene020385:CDS | 45.0% | |
| ! | CATCAACGATAACGGTGACA+AGG | + | chr7.2:13254486-13254505 | MS.gene020385:CDS | 45.0% |
| ! | CCTTTTCCCCACAGTGATTT+TGG | + | chr7.2:13255503-13255522 | MS.gene020385:intron | 45.0% |
| ! | CTGATGCATTTTCCGTGTTG+GGG | + | chr7.2:13255609-13255628 | MS.gene020385:intron | 45.0% |
| ! | TGACACGCATCCAATTTTCG+AGG | + | chr7.2:13254851-13254870 | MS.gene020385:intron | 45.0% |
| !! | AATACTTTGGAGCTGGATGC+TGG | - | chr7.2:13256053-13256072 | None:intergenic | 45.0% |
| !! | ATACTTTGGAGCTGGATGCT+GGG | - | chr7.2:13256052-13256071 | None:intergenic | 45.0% |
| !! | GGGAAAAGGTCTTTGAAGCA+TGG | - | chr7.2:13255492-13255511 | None:intergenic | 45.0% |
| GCTACTCAATCTGCTCATCC+AGG | + | chr7.2:13254635-13254654 | MS.gene020385:CDS | 50.0% | |
| TCAACACGCGTCAATCTCCA+AGG | - | chr7.2:13255343-13255362 | None:intergenic | 50.0% | |
| TGAAAACCGGGCTGCAGTTT+CGG | - | chr7.2:13255968-13255987 | None:intergenic | 50.0% | |
| TGCAGAAAGAGTCACCGCTT+GGG | + | chr7.2:13254609-13254628 | MS.gene020385:intron | 50.0% | |
| TGGAGATTGACGCGTGTTGA+TGG | + | chr7.2:13255343-13255362 | MS.gene020385:intron | 50.0% | |
| TTGCAGAAAGAGTCACCGCT+TGG | + | chr7.2:13254608-13254627 | MS.gene020385:intron | 50.0% | |
| ! | TGTTTTCCGAAACTGCAGCC+CGG | + | chr7.2:13255959-13255978 | MS.gene020385:intron | 50.0% |
| ! | TTTGGAGCTGGATGCTGGGT+TGG | - | chr7.2:13256048-13256067 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 13254469 | 13256074 | 13254469 | ID=MS.gene020385 |
| chr7.2 | mRNA | 13254469 | 13256074 | 13254469 | ID=MS.gene020385.t1;Parent=MS.gene020385 |
| chr7.2 | exon | 13254469 | 13254522 | 13254469 | ID=MS.gene020385.t1.exon1;Parent=MS.gene020385.t1 |
| chr7.2 | CDS | 13254469 | 13254522 | 13254469 | ID=cds.MS.gene020385.t1;Parent=MS.gene020385.t1 |
| chr7.2 | exon | 13254614 | 13254656 | 13254614 | ID=MS.gene020385.t1.exon2;Parent=MS.gene020385.t1 |
| chr7.2 | CDS | 13254614 | 13254656 | 13254614 | ID=cds.MS.gene020385.t1;Parent=MS.gene020385.t1 |
| chr7.2 | exon | 13255977 | 13256074 | 13255977 | ID=MS.gene020385.t1.exon3;Parent=MS.gene020385.t1 |
| chr7.2 | CDS | 13255977 | 13256074 | 13255977 | ID=cds.MS.gene020385.t1;Parent=MS.gene020385.t1 |
| Gene Sequence |
| Protein sequence |
