Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047882.t1 | XP_003629390.1 | 95 | 161 | 7 | 1 | 1 | 161 | 1 | 160 | 1.60E-81 | 312 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047882.t1 | P17093 | 91.9 | 161 | 11 | 2 | 1 | 161 | 1 | 159 | 3.4e-80 | 298.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047882.t1 | B7FGL2 | 95.0 | 161 | 7 | 1 | 1 | 161 | 1 | 160 | 1.2e-81 | 312.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047882.t1 | MTR_8g076830 | 95.031 | 161 | 7 | 1 | 1 | 161 | 1 | 160 | 3.44e-101 | 287 |
| MS.gene047882.t1 | MTR_4g116410 | 92.547 | 161 | 11 | 1 | 1 | 161 | 1 | 160 | 8.12e-99 | 281 |
| MS.gene047882.t1 | MTR_1g017780 | 88.889 | 162 | 16 | 2 | 1 | 161 | 1 | 161 | 5.68e-93 | 267 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047882.t1 | AT3G48930 | 86.957 | 161 | 20 | 1 | 1 | 161 | 1 | 160 | 6.49e-94 | 269 |
| MS.gene047882.t1 | AT5G23740 | 86.335 | 161 | 20 | 2 | 1 | 161 | 1 | 159 | 1.28e-90 | 261 |
| MS.gene047882.t1 | AT4G30800 | 86.335 | 161 | 20 | 2 | 1 | 161 | 1 | 159 | 3.47e-90 | 259 |
Find 0 sgRNAs with CRISPR-Local
Find 119 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTGAAAAATAATATCAA+TGG | - | 57553:1805-1824 | MS.gene047882:intergenic | 10.0% |
| !! | ATAAAAAAAAAAACAGAAAT+TGG | - | 57553:1899-1918 | MS.gene047882:intergenic | 10.0% |
| !! | TAAAAAAAAAAACAGAAATT+GGG | - | 57553:1898-1917 | MS.gene047882:intergenic | 10.0% |
| !!! | GTTTGTTAAATAAATAATAG+TGG | + | 57553:2589-2608 | MS.gene047882:intron | 15.0% |
| !! | ATTTATTTAACAAACACAAC+AGG | - | 57553:2584-2603 | MS.gene047882:intergenic | 20.0% |
| !! | CTAAAAAAAAATGTAGTCTT+TGG | + | 57553:2258-2277 | MS.gene047882:intron | 20.0% |
| !! | TAAAGCAAAATTGAGTTAAA+TGG | + | 57553:2312-2331 | MS.gene047882:intron | 20.0% |
| !! | TGTCTGTTTATATGATAAAT+TGG | + | 57553:2554-2573 | MS.gene047882:intron | 20.0% |
| !!! | ATATATGTATGTTTTGATAC+TGG | + | 57553:2433-2452 | MS.gene047882:intron | 20.0% |
| !!! | CAAAGACTACATTTTTTTTT+AGG | - | 57553:2260-2279 | MS.gene047882:intergenic | 20.0% |
| !!! | GAAGGTAATGTTTAATTTTA+GGG | + | 57553:2067-2086 | MS.gene047882:intron | 20.0% |
| !!! | TATGTTCTAAACTTACATAT+AGG | + | 57553:2102-2121 | MS.gene047882:intron | 20.0% |
| !!! | TGAAGGTAATGTTTAATTTT+AGG | + | 57553:2066-2085 | MS.gene047882:intron | 20.0% |
| !!! | TGGCATTTTTTTTATAAAAC+AGG | + | 57553:3139-3158 | MS.gene047882:intron | 20.0% |
| ! | AAACTTACATATAGGAATGT+AGG | + | 57553:2110-2129 | MS.gene047882:intron | 25.0% |
| ! | AAATTAAACATTACCTTCAG+TGG | - | 57553:2065-2084 | MS.gene047882:intergenic | 25.0% |
| ! | AAATTTCACTTTCAAAGTAC+TGG | - | 57553:3041-3060 | MS.gene047882:intergenic | 25.0% |
| ! | AACTTACATATAGGAATGTA+GGG | + | 57553:2111-2130 | MS.gene047882:intron | 25.0% |
| ! | ATTGAATTCCTTTCATACTA+TGG | + | 57553:3308-3327 | MS.gene047882:intron | 25.0% |
| ! | CTAAGTGTGCAATAAAAAAT+GGG | - | 57553:3336-3355 | MS.gene047882:intergenic | 25.0% |
| ! | TAAGTGTGCAATAAAAAATG+GGG | - | 57553:3335-3354 | MS.gene047882:intergenic | 25.0% |
| ! | TCTAAGTGTGCAATAAAAAA+TGG | - | 57553:3337-3356 | MS.gene047882:intergenic | 25.0% |
| ! | TTAGATAAGGATAGAAACAT+TGG | + | 57553:2351-2370 | MS.gene047882:intron | 25.0% |
| ! | TTCTATAGAAAGCAAAAGAA+AGG | - | 57553:3513-3532 | MS.gene047882:intergenic | 25.0% |
| !! | GCAATACTTATTCTAGTTAT+TGG | + | 57553:2633-2652 | MS.gene047882:intron | 25.0% |
| !! | GTATTGAGACATGTAATTTT+TGG | + | 57553:2718-2737 | MS.gene047882:intron | 25.0% |
| !!! | ACTTTATTAAGAAGTATCAG+AGG | + | 57553:3284-3303 | MS.gene047882:CDS | 25.0% |
| !!! | AGACATGTAATTTTTGGTTA+TGG | + | 57553:2724-2743 | MS.gene047882:intron | 25.0% |
| !!! | TTCTGTTTCTAATACAGAAA+TGG | + | 57553:3119-3138 | MS.gene047882:intron | 25.0% |
| ACACTTGCAAGACATAATAT+AGG | - | 57553:2813-2832 | MS.gene047882:intergenic | 30.0% | |
| ACAGAGGAGTAACAAAAAAA+GGG | - | 57553:3376-3395 | MS.gene047882:intergenic | 30.0% | |
| ACATACCATAGAAACACTTT+GGG | - | 57553:1863-1882 | MS.gene047882:intergenic | 30.0% | |
| ATAGACCAAATTCTTTCAAG+CGG | - | 57553:2768-2787 | MS.gene047882:intergenic | 30.0% | |
| ATGTTATTATTGGACAATGC+AGG | + | 57553:3468-3487 | MS.gene047882:CDS | 30.0% | |
| GGTTATGGTATGTTATGTTA+CGG | + | 57553:2739-2758 | MS.gene047882:intron | 30.0% | |
| TACATACCATAGAAACACTT+TGG | - | 57553:1864-1883 | MS.gene047882:intergenic | 30.0% | |
| TAGAGTAGTTGCACAAATAA+AGG | - | 57553:3590-3609 | MS.gene047882:intergenic | 30.0% | |
| TATGCAAGCACAATTAGATA+AGG | + | 57553:2338-2357 | MS.gene047882:intron | 30.0% | |
| TGAATAGGACTATTATTGTC+AGG | + | 57553:3248-3267 | MS.gene047882:CDS | 30.0% | |
| TGCATTGTCCAATAATAACA+TGG | - | 57553:3469-3488 | MS.gene047882:intergenic | 30.0% | |
| TTTATTTGTGCAACTACTCT+AGG | + | 57553:3589-3608 | MS.gene047882:intron | 30.0% | |
| ! | ATTGTTAATAGCTCTGTGTA+TGG | + | 57553:3081-3100 | MS.gene047882:intron | 30.0% |
| ! | TTTGTTAATAGCTCTGTGTA+TGG | + | 57553:2926-2945 | MS.gene047882:intron | 30.0% |
| !! | AGTCCTCAAATGTATTGATT+TGG | + | 57553:2182-2201 | MS.gene047882:intron | 30.0% |
| !! | GTCCTCAAATGTATTGATTT+GGG | + | 57553:2183-2202 | MS.gene047882:intron | 30.0% |
| !! | TCCTCAAATGTATTGATTTG+GGG | + | 57553:2184-2203 | MS.gene047882:intron | 30.0% |
| AAGATACAGAGATAGAACGA+AGG | - | 57553:1774-1793 | MS.gene047882:intergenic | 35.0% | |
| AATCGTTACTGGAAATCAGT+TGG | + | 57553:2013-2032 | MS.gene047882:CDS | 35.0% | |
| AGTTCGAAGAAATCTGTAAG+GGG | + | 57553:1969-1988 | MS.gene047882:intron | 35.0% | |
| ATAGGACTATTATTGTCAGG+AGG | + | 57553:3251-3270 | MS.gene047882:CDS | 35.0% | |
| CAGTTCGAAGAAATCTGTAA+GGG | + | 57553:1968-1987 | MS.gene047882:intron | 35.0% | |
| GAAGGAGACCATGTTATTAT+TGG | + | 57553:3458-3477 | MS.gene047882:CDS | 35.0% | |
| GACAGAGGAGTAACAAAAAA+AGG | - | 57553:3377-3396 | MS.gene047882:intergenic | 35.0% | |
| TCCCCAAATCAATACATTTG+AGG | - | 57553:2188-2207 | MS.gene047882:intergenic | 35.0% | |
| TCGTTCTATCTCTGTATCTT+TGG | + | 57553:1774-1793 | MS.gene047882:intron | 35.0% | |
| TTCTTTGTTACAGACTGAGA+AGG | + | 57553:1823-1842 | MS.gene047882:intron | 35.0% | |
| ! | AATAGCTCTGTGTATGGTTA+CGG | + | 57553:2932-2951 | MS.gene047882:intron | 35.0% |
| ! | TATGTCTTGCAAGTGTTGTT+GGG | + | 57553:2817-2836 | MS.gene047882:intron | 35.0% |
| ! | TGTAGGGATAAACAGCATTT+TGG | + | 57553:2127-2146 | MS.gene047882:intron | 35.0% |
| ! | TTATGTCTTGCAAGTGTTGT+TGG | + | 57553:2816-2835 | MS.gene047882:intron | 35.0% |
| ! | TTTTTGTTACTCCTCTGTCT+AGG | + | 57553:3378-3397 | MS.gene047882:intron | 35.0% |
| !!! | ACGCTATTTCTTTTGCATAG+TGG | + | 57553:2390-2409 | MS.gene047882:intron | 35.0% |
| AAGAAATCTGTAAGGGGAAG+AGG | + | 57553:1975-1994 | MS.gene047882:CDS | 40.0% | |
| AATGGGGACCATAGTATGAA+AGG | - | 57553:3319-3338 | MS.gene047882:intergenic | 40.0% | |
| CAATGCAGGTGAGACTTTAT+AGG | + | 57553:3482-3501 | MS.gene047882:intron | 40.0% | |
| GAAAAGGTGGAAATCGTTAC+TGG | + | 57553:2002-2021 | MS.gene047882:CDS | 40.0% | |
| GCAGTTCGAAGAAATCTGTA+AGG | + | 57553:1967-1986 | MS.gene047882:intron | 40.0% | |
| GGTATCCGCTTGAAAGAATT+TGG | + | 57553:2760-2779 | MS.gene047882:intron | 40.0% | |
| TAATAGAGCCTCACATTCGA+GGG | + | 57553:2218-2237 | MS.gene047882:intron | 40.0% | |
| TCCTATTCATCTTAGCACTG+TGG | - | 57553:3237-3256 | MS.gene047882:intergenic | 40.0% | |
| TGGACACTTCTTGTCAATGT+AGG | - | 57553:3167-3186 | MS.gene047882:intergenic | 40.0% | |
| ! | AGCAACCCAAAGTGTTTCTA+TGG | + | 57553:1855-1874 | MS.gene047882:CDS | 40.0% |
| ! | TAACGATTTCCACCTTTTCC+AGG | - | 57553:2001-2020 | MS.gene047882:intergenic | 40.0% |
| ! | TACTGGAAATCAGTTGGTCT+TGG | + | 57553:2019-2038 | MS.gene047882:CDS | 40.0% |
| ! | TGTTTGTCTGAGATTCCTCT+TGG | + | 57553:2481-2500 | MS.gene047882:intron | 40.0% |
| ATCACCTTGCTTCCGTGTTA+AGG | + | 57553:3436-3455 | MS.gene047882:CDS | 45.0% | |
| ATCTAGTGGTGCAGGAAAGA+AGG | + | 57553:3665-3684 | MS.gene047882:CDS | 45.0% | |
| ATGGAAACATTGCCGGTGAA+TGG | - | 57553:3187-3206 | MS.gene047882:intergenic | 45.0% | |
| ATTACCTTCAGTGGCTTCTC+TGG | - | 57553:2056-2075 | MS.gene047882:intergenic | 45.0% | |
| ATTCCTCTTGGCATATCAGG+TGG | + | 57553:2493-2512 | MS.gene047882:intron | 45.0% | |
| CATGGTCTCCTTCCTTAACA+CGG | - | 57553:3451-3470 | MS.gene047882:intergenic | 45.0% | |
| CTCTGTCTAGGTATGAGAAG+AGG | + | 57553:3390-3409 | MS.gene047882:intron | 45.0% | |
| CTCTTCTCATACCTAGACAG+AGG | - | 57553:3392-3411 | MS.gene047882:intergenic | 45.0% | |
| GAGATTCCTCTTGGCATATC+AGG | + | 57553:2490-2509 | MS.gene047882:intron | 45.0% | |
| GCCACAGTGCTAAGATGAAT+AGG | + | 57553:3233-3252 | MS.gene047882:CDS | 45.0% | |
| GTAATAGAGCCTCACATTCG+AGG | + | 57553:2217-2236 | MS.gene047882:intron | 45.0% | |
| TTACCTTCAGTGGCTTCTCT+GGG | - | 57553:2055-2074 | MS.gene047882:intergenic | 45.0% | |
| ! | GCAGGAAAGAAGGCTTTTAC+TGG | + | 57553:3675-3694 | MS.gene047882:CDS | 45.0% |
| ! | TCTTGCAAGTGTTGTTGGGA+AGG | + | 57553:2821-2840 | MS.gene047882:intron | 45.0% |
| !! | CTGAGCGTCTAGCTTTGTAT+TGG | + | 57553:2456-2475 | MS.gene047882:intron | 45.0% |
| !! | GACAAGAAGTGTCCATTCAC+CGG | + | 57553:3172-3191 | MS.gene047882:CDS | 45.0% |
| AACCTCACAGTCTTGGAGAG+TGG | - | 57553:3615-3634 | None:intergenic | 50.0% | |
| AAGCAAGGTGATACGTGAGC+GGG | - | 57553:3428-3447 | MS.gene047882:intergenic | 50.0% | |
| ATCCCAGCTGGATCATCTAG+TGG | + | 57553:3651-3670 | MS.gene047882:CDS | 50.0% | |
| CACCACTAGATGATCCAGCT+GGG | - | 57553:3656-3675 | MS.gene047882:intergenic | 50.0% | |
| CACGTTGAACCTCACAGTCT+TGG | - | 57553:3622-3641 | MS.gene047882:intergenic | 50.0% | |
| CCTTCCTTAACACGGAAGCA+AGG | - | 57553:3443-3462 | MS.gene047882:intergenic | 50.0% | |
| CCTTGCTTCCGTGTTAAGGA+AGG | + | 57553:3440-3459 | MS.gene047882:CDS | 50.0% | |
| CTTAGCACTGTGGCAAGTTC+CGG | - | 57553:3227-3246 | MS.gene047882:intergenic | 50.0% | |
| GAAGCAAGGTGATACGTGAG+CGG | - | 57553:3429-3448 | MS.gene047882:intergenic | 50.0% | |
| GTGTTGAAAGTGATCCCAGC+TGG | + | 57553:3639-3658 | MS.gene047882:CDS | 50.0% | |
| GTTATGCTCCCTCGAATGTG+AGG | - | 57553:2229-2248 | MS.gene047882:intergenic | 50.0% | |
| TACCTTCAGTGGCTTCTCTG+GGG | - | 57553:2054-2073 | MS.gene047882:intergenic | 50.0% | |
| TAGCCACCTGATATGCCAAG+AGG | - | 57553:2499-2518 | MS.gene047882:intergenic | 50.0% | |
| TCACCGGCAATGTTTCCATC+CGG | + | 57553:3188-3207 | MS.gene047882:CDS | 50.0% | |
| AAGGGGAAGAGGCCTGGAAA+AGG | + | 57553:1986-2005 | MS.gene047882:CDS | 55.0% | |
| ACCCCGGATGGAAACATTGC+CGG | - | 57553:3194-3213 | MS.gene047882:intergenic | 55.0% | |
| ACCGGCAATGTTTCCATCCG+GGG | + | 57553:3190-3209 | MS.gene047882:CDS | 55.0% | |
| ACCTTCAGTGGCTTCTCTGG+GGG | - | 57553:2053-2072 | MS.gene047882:intergenic | 55.0% | |
| ATCCGGGGTCGTATCTTAGC+CGG | + | 57553:3205-3224 | MS.gene047882:CDS | 55.0% | |
| ATCTGTAAGGGGAAGAGGCC+TGG | + | 57553:1980-1999 | MS.gene047882:CDS | 55.0% | |
| CACCGGCAATGTTTCCATCC+GGG | + | 57553:3189-3208 | MS.gene047882:CDS | 55.0% | |
| GCACCACTAGATGATCCAGC+TGG | - | 57553:3657-3676 | MS.gene047882:intergenic | 55.0% | |
| GCTGGATCATCTAGTGGTGC+AGG | + | 57553:3657-3676 | MS.gene047882:CDS | 55.0% | |
| TTCCGGCTAAGATACGACCC+CGG | - | 57553:3210-3229 | MS.gene047882:intergenic | 55.0% | |
| ACCCCCAGAGAAGCCACTGA+AGG | + | 57553:2049-2068 | MS.gene047882:CDS | 60.0% | |
| GGCCACTCTCCAAGACTGTG+AGG | + | 57553:3610-3629 | MS.gene047882:intron | 60.0% | |
| GGCTAAGATACGACCCCGGA+TGG | - | 57553:3206-3225 | MS.gene047882:intergenic | 60.0% | |
| GGGAAGAGGCCTGGAAAAGG+TGG | + | 57553:1989-2008 | MS.gene047882:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57553 | gene | 1739 | 3704 | 1739 | ID=MS.gene047882 |
| 57553 | mRNA | 1739 | 3704 | 1739 | ID=MS.gene047882.t1;Parent=MS.gene047882 |
| 57553 | exon | 1739 | 1750 | 1739 | ID=MS.gene047882.t1.exon1;Parent=MS.gene047882.t1 |
| 57553 | CDS | 1739 | 1750 | 1739 | ID=cds.MS.gene047882.t1;Parent=MS.gene047882.t1 |
| 57553 | exon | 1836 | 1880 | 1836 | ID=MS.gene047882.t1.exon2;Parent=MS.gene047882.t1 |
| 57553 | CDS | 1836 | 1880 | 1836 | ID=cds.MS.gene047882.t1;Parent=MS.gene047882.t1 |
| 57553 | exon | 1971 | 2070 | 1971 | ID=MS.gene047882.t1.exon3;Parent=MS.gene047882.t1 |
| 57553 | CDS | 1971 | 2070 | 1971 | ID=cds.MS.gene047882.t1;Parent=MS.gene047882.t1 |
| 57553 | exon | 3161 | 3305 | 3161 | ID=MS.gene047882.t1.exon4;Parent=MS.gene047882.t1 |
| 57553 | CDS | 3161 | 3305 | 3161 | ID=cds.MS.gene047882.t1;Parent=MS.gene047882.t1 |
| 57553 | exon | 3400 | 3489 | 3400 | ID=MS.gene047882.t1.exon5;Parent=MS.gene047882.t1 |
| 57553 | CDS | 3400 | 3489 | 3400 | ID=cds.MS.gene047882.t1;Parent=MS.gene047882.t1 |
| 57553 | exon | 3611 | 3704 | 3611 | ID=MS.gene047882.t1.exon6;Parent=MS.gene047882.t1 |
| 57553 | CDS | 3611 | 3704 | 3611 | ID=cds.MS.gene047882.t1;Parent=MS.gene047882.t1 |
| Gene Sequence |
| Protein sequence |
