Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074594.t1 | XP_003629390.1 | 100 | 160 | 0 | 0 | 1 | 160 | 1 | 160 | 5.70E-87 | 330.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074594.t1 | P17093 | 95.0 | 160 | 7 | 1 | 1 | 160 | 1 | 159 | 8.6e-84 | 310.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074594.t1 | B7FGL2 | 100.0 | 160 | 0 | 0 | 1 | 160 | 1 | 160 | 4.1e-87 | 330.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050277 | MS.gene074594 | 0.807173 | 5.75E-50 | -1.69E-46 |
| MS.gene050290 | MS.gene074594 | 0.902786 | 6.79E-79 | -1.69E-46 |
| MS.gene050916 | MS.gene074594 | 0.860262 | 2.33E-63 | -1.69E-46 |
| MS.gene051330 | MS.gene074594 | 0.807745 | 4.35E-50 | -1.69E-46 |
| MS.gene051850 | MS.gene074594 | 0.816429 | 5.56E-52 | -1.69E-46 |
| MS.gene051903 | MS.gene074594 | 0.816866 | 4.43E-52 | -1.69E-46 |
| MS.gene051904 | MS.gene074594 | 0.807467 | 4.98E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene074594 | 0.87209 | 4.15E-67 | -1.69E-46 |
| MS.gene056601 | MS.gene074594 | 0.827475 | 1.53E-54 | -1.69E-46 |
| MS.gene056917 | MS.gene074594 | 0.814491 | 1.50E-51 | -1.69E-46 |
| MS.gene057052 | MS.gene074594 | 0.811705 | 6.13E-51 | -1.69E-46 |
| MS.gene058906 | MS.gene074594 | 0.80186 | 7.38E-49 | -1.69E-46 |
| MS.gene06001 | MS.gene074594 | 0.858419 | 8.34E-63 | -1.69E-46 |
| MS.gene060856 | MS.gene074594 | 0.80978 | 1.60E-50 | -1.69E-46 |
| MS.gene061145 | MS.gene074594 | 0.811836 | 5.74E-51 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074594.t1 | MTR_8g076830 | 100.000 | 160 | 0 | 0 | 1 | 160 | 1 | 160 | 1.21e-117 | 329 |
| MS.gene074594.t1 | MTR_4g116410 | 97.500 | 160 | 4 | 0 | 1 | 160 | 1 | 160 | 6.73e-115 | 322 |
| MS.gene074594.t1 | MTR_1g017780 | 93.168 | 161 | 10 | 1 | 1 | 160 | 1 | 161 | 2.44e-99 | 283 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074594.t1 | AT3G48930 | 91.250 | 160 | 14 | 0 | 1 | 160 | 1 | 160 | 6.64e-98 | 279 |
| MS.gene074594.t1 | AT5G23740 | 90.000 | 160 | 15 | 1 | 1 | 160 | 1 | 159 | 4.99e-96 | 274 |
| MS.gene074594.t1 | AT4G30800 | 90.625 | 160 | 14 | 1 | 1 | 160 | 1 | 159 | 4.19e-94 | 270 |
Find 0 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTGAAAAATAATATCAA+TGG | - | 57557:1142-1161 | MS.gene074594:intergenic | 10.0% |
| !! | AATAAAAAAAAAACAGAAAT+TGG | - | 57557:1236-1255 | MS.gene074594:intergenic | 10.0% |
| !! | ATAAAAAAAAAACAGAAATT+GGG | - | 57557:1235-1254 | MS.gene074594:intergenic | 10.0% |
| !! | TAAAAAAAAAATGTAGTCTT+TGG | + | 57557:1598-1617 | MS.gene074594:intron | 15.0% |
| !! | TGTTTGTAAATAAATAATAG+TGG | + | 57557:1931-1950 | MS.gene074594:intron | 15.0% |
| !!! | AAAGACTACATTTTTTTTTT+AGG | - | 57557:1599-1618 | MS.gene074594:intergenic | 15.0% |
| !! | TAAAGCAAAATTGAGTTAAA+TGG | + | 57557:1652-1671 | MS.gene074594:intron | 20.0% |
| !! | TAAGTGTGCAAATAAAAAAT+GGG | - | 57557:2681-2700 | MS.gene074594:intergenic | 20.0% |
| !! | TATTTATTTACAAACACAAC+AGG | - | 57557:1927-1946 | MS.gene074594:intergenic | 20.0% |
| !! | TGTCTGTTTATATGATAAAT+TGG | + | 57557:1897-1916 | MS.gene074594:intron | 20.0% |
| !!! | ATTTATGTATGTTTTGATAC+TGG | + | 57557:1774-1793 | MS.gene074594:intron | 20.0% |
| !!! | GAAGGTAATGTTTAATTTTA+GGG | + | 57557:1404-1423 | MS.gene074594:intron | 20.0% |
| !!! | GGCATTTTTTTTTATAAAAC+AGG | + | 57557:2484-2503 | MS.gene074594:intron | 20.0% |
| !!! | TATGTTCTAAACTTACATAT+AGG | + | 57557:1439-1458 | MS.gene074594:intron | 20.0% |
| !!! | TGAAGGTAATGTTTAATTTT+AGG | + | 57557:1403-1422 | MS.gene074594:intron | 20.0% |
| ! | AAACTTACATATAGGAATGT+AGG | + | 57557:1447-1466 | MS.gene074594:intron | 25.0% |
| ! | AAATTAAACATTACCTTCAG+TGG | - | 57557:1402-1421 | MS.gene074594:intergenic | 25.0% |
| ! | AACTAAAACAAATACACTCA+AGG | - | 57557:1491-1510 | MS.gene074594:intergenic | 25.0% |
| ! | AACTTACATATAGGAATGTA+GGG | + | 57557:1448-1467 | MS.gene074594:intron | 25.0% |
| ! | AAGTGTGCAAATAAAAAATG+GGG | - | 57557:2680-2699 | MS.gene074594:intergenic | 25.0% |
| ! | AATTTCACTTTCAAAGTAAC+TGG | - | 57557:2228-2247 | MS.gene074594:intergenic | 25.0% |
| ! | ATTGAATTCCTTTCATACTA+TGG | + | 57557:2653-2672 | MS.gene074594:intron | 25.0% |
| ! | CTAAGTGTGCAAATAAAAAA+TGG | - | 57557:2682-2701 | MS.gene074594:intergenic | 25.0% |
| ! | GTTATGGTTATGTTATGTTA+CGG | + | 57557:2082-2101 | MS.gene074594:intron | 25.0% |
| ! | TTAGATAAGGATAGAAACAT+TGG | + | 57557:1692-1711 | MS.gene074594:intron | 25.0% |
| ! | TTCTATAGAAAGCAAAAGAA+AGG | - | 57557:2859-2878 | MS.gene074594:intergenic | 25.0% |
| !! | GCAATACTTATTCTAGTTAT+TGG | + | 57557:1975-1994 | MS.gene074594:intron | 25.0% |
| !! | GTATTGAGACATGTAATTTT+TGG | + | 57557:2060-2079 | MS.gene074594:intron | 25.0% |
| !!! | ACTTTATTAAGAAGTATCAG+AGG | + | 57557:2629-2648 | MS.gene074594:CDS | 25.0% |
| !!! | AGACATGTAATTTTTGGTTA+TGG | + | 57557:2066-2085 | MS.gene074594:intron | 25.0% |
| !!! | ATTTTAGTCCTCAAATGTAT+TGG | + | 57557:1515-1534 | MS.gene074594:intron | 25.0% |
| !!! | TTCTGTTTCTAATACAGAAA+TGG | + | 57557:2463-2482 | MS.gene074594:intron | 25.0% |
| ACACTTGCAAGACATAATAT+AGG | - | 57557:2155-2174 | MS.gene074594:intergenic | 30.0% | |
| ACAGAGGAGTAACAAAAAAA+GGG | - | 57557:2722-2741 | MS.gene074594:intergenic | 30.0% | |
| ACATACCATAGAAACACTTT+GGG | - | 57557:1200-1219 | MS.gene074594:intergenic | 30.0% | |
| ATGCAAAGCACAATTAGATA+AGG | + | 57557:1679-1698 | MS.gene074594:intron | 30.0% | |
| ATGTTATTATTGGACAATGC+AGG | + | 57557:2814-2833 | MS.gene074594:CDS | 30.0% | |
| TACATACCATAGAAACACTT+TGG | - | 57557:1201-1220 | MS.gene074594:intergenic | 30.0% | |
| TAGAGTAGTTGCACAAATAA+AGG | - | 57557:2936-2955 | MS.gene074594:intergenic | 30.0% | |
| TATAGACCAATTCTTTCAAG+CGG | - | 57557:2111-2130 | MS.gene074594:intergenic | 30.0% | |
| TCCTCAAATGTATTGGATTT+GGG | + | 57557:1522-1541 | MS.gene074594:intron | 30.0% | |
| TGAATAGGACTATTATTGTC+AGG | + | 57557:2593-2612 | MS.gene074594:CDS | 30.0% | |
| TGCATTGTCCAATAATAACA+TGG | - | 57557:2815-2834 | MS.gene074594:intergenic | 30.0% | |
| TTTATTTGTGCAACTACTCT+AGG | + | 57557:2935-2954 | MS.gene074594:intron | 30.0% | |
| ! | ATTGTTAATAGCTCTGTGTA+TGG | + | 57557:2269-2288 | MS.gene074594:intron | 30.0% |
| AAGATACAGAGATAGAACGA+AGG | - | 57557:1111-1130 | MS.gene074593:intergenic | 35.0% | |
| AGTTCGAAGAAATCTGGTAA+GGG | + | 57557:1305-1324 | MS.gene074594:intron | 35.0% | |
| ATAGGACTATTATTGTCAGG+AGG | + | 57557:2596-2615 | MS.gene074594:CDS | 35.0% | |
| CCTCAAATGTATTGGATTTG+GGG | + | 57557:1523-1542 | MS.gene074594:intron | 35.0% | |
| GAAGGAGACCATGTTATTAT+TGG | + | 57557:2804-2823 | MS.gene074594:CDS | 35.0% | |
| GACAGAGGAGTAACAAAAAA+AGG | - | 57557:2723-2742 | MS.gene074594:intergenic | 35.0% | |
| GTCCTCAAATGTATTGGATT+TGG | + | 57557:1521-1540 | MS.gene074594:intron | 35.0% | |
| TCGTTCTATCTCTGTATCTT+TGG | + | 57557:1111-1130 | MS.gene074594:intron | 35.0% | |
| TTCTTTGTTACAGACTGAGA+AGG | + | 57557:1160-1179 | MS.gene074594:intron | 35.0% | |
| ! | AATAGCTCTGTGTATGGTTA+CGG | + | 57557:2275-2294 | MS.gene074594:intron | 35.0% |
| ! | AATCGTTTCTGGAAATCAGT+TGG | + | 57557:1350-1369 | MS.gene074594:CDS | 35.0% |
| ! | TATGTCTTGCAAGTGTTGTT+GGG | + | 57557:2159-2178 | MS.gene074594:intron | 35.0% |
| ! | TGTAGGGATAAACAGCATTT+TGG | + | 57557:1464-1483 | MS.gene074594:intron | 35.0% |
| ! | TTATGTCTTGCAAGTGTTGT+TGG | + | 57557:2158-2177 | MS.gene074594:intron | 35.0% |
| ! | TTTTTGTTACTCCTCTGTCT+AGG | + | 57557:2724-2743 | MS.gene074594:intron | 35.0% |
| !!! | ACGCTATTTCTTTTGCATAG+TGG | + | 57557:1731-1750 | MS.gene074594:intron | 35.0% |
| AATGGGGACCATAGTATGAA+AGG | - | 57557:2664-2683 | MS.gene074594:intergenic | 40.0% | |
| AGATTCCTCTTGGCAATATC+AGG | + | 57557:1832-1851 | MS.gene074594:intron | 40.0% | |
| CAATGCAGGTGAGACTTTAT+AGG | + | 57557:2828-2847 | MS.gene074594:intron | 40.0% | |
| CAGTTCGAAGAAATCTGGTA+AGG | + | 57557:1304-1323 | MS.gene074594:intron | 40.0% | |
| CCCCAAATCCAATACATTTG+AGG | - | 57557:1526-1545 | MS.gene074594:intergenic | 40.0% | |
| GAAAAGGTGGAAATCGTTTC+TGG | + | 57557:1339-1358 | MS.gene074594:CDS | 40.0% | |
| GTTCGAAGAAATCTGGTAAG+GGG | + | 57557:1306-1325 | MS.gene074594:intron | 40.0% | |
| TAATAGAGCCTCACATTCGA+GGG | + | 57557:1557-1576 | MS.gene074594:intron | 40.0% | |
| TCCTATTCATCTTAGCACTG+TGG | - | 57557:2582-2601 | MS.gene074594:intergenic | 40.0% | |
| TGGACACTTCTTGTCAATGT+AGG | - | 57557:2512-2531 | MS.gene074594:intergenic | 40.0% | |
| TGTTGCAGTTCGAAGAAATC+TGG | + | 57557:1299-1318 | MS.gene074594:intron | 40.0% | |
| ! | AAACGATTTCCACCTTTTCC+AGG | - | 57557:1338-1357 | MS.gene074594:intergenic | 40.0% |
| ! | AGCAACCCAAAGTGTTTCTA+TGG | + | 57557:1192-1211 | MS.gene074594:CDS | 40.0% |
| ! | TGTTTGTCTGAGATTCCTCT+TGG | + | 57557:1822-1841 | MS.gene074594:intron | 40.0% |
| ! | TTCTGGAAATCAGTTGGTCT+TGG | + | 57557:1356-1375 | MS.gene074594:CDS | 40.0% |
| AGAAATCTGGTAAGGGGAAG+AGG | + | 57557:1312-1331 | MS.gene074594:CDS | 45.0% | |
| ATCACCTTGCTTCCGTGTTA+AGG | + | 57557:2782-2801 | MS.gene074594:CDS | 45.0% | |
| ATCTAGTGGTGCAGGAAAGA+AGG | + | 57557:3011-3030 | MS.gene074594:CDS | 45.0% | |
| ATGGAAACATTGCCGGTGAA+TGG | - | 57557:2532-2551 | MS.gene074594:intergenic | 45.0% | |
| ATTACCTTCAGTGGCTTCTC+TGG | - | 57557:1393-1412 | MS.gene074594:intergenic | 45.0% | |
| CATGGTCTCCTTCCTTAACA+CGG | - | 57557:2797-2816 | MS.gene074594:intergenic | 45.0% | |
| CGGTATCCGCTTGAAAGAAT+TGG | + | 57557:2102-2121 | MS.gene074594:intron | 45.0% | |
| CTCTGTCTAGGTATGAGAAG+AGG | + | 57557:2736-2755 | MS.gene074594:intron | 45.0% | |
| CTCTTCTCATACCTAGACAG+AGG | - | 57557:2738-2757 | MS.gene074594:intergenic | 45.0% | |
| GCCACAGTGCTAAGATGAAT+AGG | + | 57557:2578-2597 | MS.gene074594:CDS | 45.0% | |
| GTAATAGAGCCTCACATTCG+AGG | + | 57557:1556-1575 | MS.gene074594:intron | 45.0% | |
| TTACCTTCAGTGGCTTCTCT+GGG | - | 57557:1392-1411 | MS.gene074594:intergenic | 45.0% | |
| TTCCTCTTGGCAATATCAGG+TGG | + | 57557:1835-1854 | MS.gene074594:intron | 45.0% | |
| ! | GCAGGAAAGAAGGCTTTTAC+TGG | + | 57557:3021-3040 | MS.gene074594:CDS | 45.0% |
| ! | TCTTGCAAGTGTTGTTGGGA+AGG | + | 57557:2163-2182 | MS.gene074594:intron | 45.0% |
| !! | CTGAGCGTCTAGCTTTGTAT+TGG | + | 57557:1797-1816 | MS.gene074594:intron | 45.0% |
| !! | GACAAGAAGTGTCCATTCAC+CGG | + | 57557:2517-2536 | MS.gene074594:CDS | 45.0% |
| AACCTCACAGTCTTGGAGAG+TGG | - | 57557:2961-2980 | MS.gene074593:intergenic | 50.0% | |
| AAGCAAGGTGATACGTGAGC+GGG | - | 57557:2774-2793 | MS.gene074593:intergenic | 50.0% | |
| AGCCACCTGATATTGCCAAG+AGG | - | 57557:1840-1859 | MS.gene074594:intergenic | 50.0% | |
| ATCCCAGCTGGATCATCTAG+TGG | + | 57557:2997-3016 | MS.gene074594:CDS | 50.0% | |
| CACCACTAGATGATCCAGCT+GGG | - | 57557:3002-3021 | MS.gene074594:intergenic | 50.0% | |
| CACGTTGAACCTCACAGTCT+TGG | - | 57557:2968-2987 | MS.gene074594:intergenic | 50.0% | |
| CCTTCCTTAACACGGAAGCA+AGG | - | 57557:2789-2808 | MS.gene074594:intergenic | 50.0% | |
| CCTTGCTTCCGTGTTAAGGA+AGG | + | 57557:2786-2805 | MS.gene074594:CDS | 50.0% | |
| CTTAGCACTGTGGCAAGTTC+CGG | - | 57557:2572-2591 | MS.gene074594:intergenic | 50.0% | |
| GAAGCAAGGTGATACGTGAG+CGG | - | 57557:2775-2794 | MS.gene074594:intergenic | 50.0% | |
| GTGTTGAAAGTGATCCCAGC+TGG | + | 57557:2985-3004 | MS.gene074594:CDS | 50.0% | |
| GTTATGCTCCCTCGAATGTG+AGG | - | 57557:1568-1587 | MS.gene074594:intergenic | 50.0% | |
| TACCTTCAGTGGCTTCTCTG+GGG | - | 57557:1391-1410 | MS.gene074594:intergenic | 50.0% | |
| TCACCGGCAATGTTTCCATC+CGG | + | 57557:2533-2552 | MS.gene074594:CDS | 50.0% | |
| AAGGGGAAGAGGCCTGGAAA+AGG | + | 57557:1323-1342 | MS.gene074594:CDS | 55.0% | |
| ACCCCGGATGGAAACATTGC+CGG | - | 57557:2539-2558 | MS.gene074594:intergenic | 55.0% | |
| ACCGGCAATGTTTCCATCCG+GGG | + | 57557:2535-2554 | MS.gene074594:CDS | 55.0% | |
| ACCTTCAGTGGCTTCTCTGG+GGG | - | 57557:1390-1409 | MS.gene074594:intergenic | 55.0% | |
| ATCCGGGGTCGTATCTTAGC+CGG | + | 57557:2550-2569 | MS.gene074594:CDS | 55.0% | |
| CACCGGCAATGTTTCCATCC+GGG | + | 57557:2534-2553 | MS.gene074594:CDS | 55.0% | |
| GCACCACTAGATGATCCAGC+TGG | - | 57557:3003-3022 | MS.gene074594:intergenic | 55.0% | |
| GCTGGATCATCTAGTGGTGC+AGG | + | 57557:3003-3022 | MS.gene074594:CDS | 55.0% | |
| TTCCGGCTAAGATACGACCC+CGG | - | 57557:2555-2574 | MS.gene074594:intergenic | 55.0% | |
| ACCCCCAGAGAAGCCACTGA+AGG | + | 57557:1386-1405 | MS.gene074594:CDS | 60.0% | |
| GGCCACTCTCCAAGACTGTG+AGG | + | 57557:2956-2975 | MS.gene074594:intron | 60.0% | |
| GGCTAAGATACGACCCCGGA+TGG | - | 57557:2551-2570 | MS.gene074594:intergenic | 60.0% | |
| GGGAAGAGGCCTGGAAAAGG+TGG | + | 57557:1326-1345 | MS.gene074594:CDS | 60.0% | |
| TCTGGTAAGGGGAAGAGGCC+TGG | + | 57557:1317-1336 | MS.gene074594:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57557 | gene | 1076 | 3050 | 1076 | ID=MS.gene074594 |
| 57557 | mRNA | 1076 | 3050 | 1076 | ID=MS.gene074594.t1;Parent=MS.gene074594 |
| 57557 | exon | 1076 | 1087 | 1076 | ID=MS.gene074594.t1.exon1;Parent=MS.gene074594.t1 |
| 57557 | CDS | 1076 | 1087 | 1076 | ID=cds.MS.gene074594.t1;Parent=MS.gene074594.t1 |
| 57557 | exon | 1173 | 1213 | 1173 | ID=MS.gene074594.t1.exon2;Parent=MS.gene074594.t1 |
| 57557 | CDS | 1173 | 1213 | 1173 | ID=cds.MS.gene074594.t1;Parent=MS.gene074594.t1 |
| 57557 | exon | 1307 | 1407 | 1307 | ID=MS.gene074594.t1.exon3;Parent=MS.gene074594.t1 |
| 57557 | CDS | 1307 | 1407 | 1307 | ID=cds.MS.gene074594.t1;Parent=MS.gene074594.t1 |
| 57557 | exon | 2506 | 2650 | 2506 | ID=MS.gene074594.t1.exon4;Parent=MS.gene074594.t1 |
| 57557 | CDS | 2506 | 2650 | 2506 | ID=cds.MS.gene074594.t1;Parent=MS.gene074594.t1 |
| 57557 | exon | 2746 | 2835 | 2746 | ID=MS.gene074594.t1.exon5;Parent=MS.gene074594.t1 |
| 57557 | CDS | 2746 | 2835 | 2746 | ID=cds.MS.gene074594.t1;Parent=MS.gene074594.t1 |
| 57557 | exon | 2957 | 3050 | 2957 | ID=MS.gene074594.t1.exon6;Parent=MS.gene074594.t1 |
| 57557 | CDS | 2957 | 3050 | 2957 | ID=cds.MS.gene074594.t1;Parent=MS.gene074594.t1 |
| Gene Sequence |
| Protein sequence |
