Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047883.t1 | XP_003629390.1 | 100 | 160 | 0 | 0 | 1 | 160 | 1 | 160 | 5.70E-87 | 330.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047883.t1 | P17093 | 95.0 | 160 | 7 | 1 | 1 | 160 | 1 | 159 | 8.6e-84 | 310.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047883.t1 | B7FGL2 | 100.0 | 160 | 0 | 0 | 1 | 160 | 1 | 160 | 4.1e-87 | 330.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047883.t1 | MTR_8g076830 | 100.000 | 160 | 0 | 0 | 1 | 160 | 1 | 160 | 1.21e-117 | 329 |
| MS.gene047883.t1 | MTR_4g116410 | 97.500 | 160 | 4 | 0 | 1 | 160 | 1 | 160 | 6.73e-115 | 322 |
| MS.gene047883.t1 | MTR_1g017780 | 93.168 | 161 | 10 | 1 | 1 | 160 | 1 | 161 | 2.44e-99 | 283 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047883.t1 | AT3G48930 | 91.250 | 160 | 14 | 0 | 1 | 160 | 1 | 160 | 6.64e-98 | 279 |
| MS.gene047883.t1 | AT5G23740 | 90.000 | 160 | 15 | 1 | 1 | 160 | 1 | 159 | 4.99e-96 | 274 |
| MS.gene047883.t1 | AT4G30800 | 90.625 | 160 | 14 | 1 | 1 | 160 | 1 | 159 | 4.19e-94 | 270 |
Find 0 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTGAAAAATAATATCAA+TGG | - | 57554:4172-4191 | MS.gene047883:intergenic | 10.0% |
| !! | ATAAAAAAAAAAACAGAAAT+TGG | - | 57554:4266-4285 | MS.gene047883:intergenic | 10.0% |
| !! | TAAAAAAAAAAACAGAAATT+GGG | - | 57554:4265-4284 | MS.gene047883:intergenic | 10.0% |
| !! | TAAAAAAAAAATGTAGTCTT+TGG | + | 57554:4629-4648 | MS.gene047883:intron | 15.0% |
| !! | TGTTTGTAAATAAATAATAG+TGG | + | 57554:4962-4981 | MS.gene047883:intron | 15.0% |
| !!! | AAAGACTACATTTTTTTTTT+AGG | - | 57554:4630-4649 | MS.gene047883:intergenic | 15.0% |
| !! | TAAAGCAAAATTGAGTTAAA+TGG | + | 57554:4683-4702 | MS.gene047883:intron | 20.0% |
| !! | TAAGTGTGCAAATAAAAAAT+GGG | - | 57554:5712-5731 | MS.gene047883:intergenic | 20.0% |
| !! | TATTTATTTACAAACACAAC+AGG | - | 57554:4958-4977 | MS.gene047883:intergenic | 20.0% |
| !! | TGTCTGTTTATATGATAAAT+TGG | + | 57554:4928-4947 | MS.gene047883:intron | 20.0% |
| !!! | ATTTATGTATGTTTTGATAC+TGG | + | 57554:4805-4824 | MS.gene047883:intron | 20.0% |
| !!! | GAAGGTAATGTTTAATTTTA+GGG | + | 57554:4435-4454 | MS.gene047883:intron | 20.0% |
| !!! | GGCATTTTTTTTTATAAAAC+AGG | + | 57554:5515-5534 | MS.gene047883:intron | 20.0% |
| !!! | TATGTTCTAAACTTACATAT+AGG | + | 57554:4470-4489 | MS.gene047883:intron | 20.0% |
| !!! | TGAAGGTAATGTTTAATTTT+AGG | + | 57554:4434-4453 | MS.gene047883:intron | 20.0% |
| ! | AAACTTACATATAGGAATGT+AGG | + | 57554:4478-4497 | MS.gene047883:intron | 25.0% |
| ! | AAATTAAACATTACCTTCAG+TGG | - | 57554:4433-4452 | MS.gene047883:intergenic | 25.0% |
| ! | AACTAAAACAAATACACTCA+AGG | - | 57554:4522-4541 | MS.gene047883:intergenic | 25.0% |
| ! | AACTTACATATAGGAATGTA+GGG | + | 57554:4479-4498 | MS.gene047883:intron | 25.0% |
| ! | AAGTGTGCAAATAAAAAATG+GGG | - | 57554:5711-5730 | MS.gene047883:intergenic | 25.0% |
| ! | AATTTCACTTTCAAAGTAAC+TGG | - | 57554:5259-5278 | MS.gene047883:intergenic | 25.0% |
| ! | ATTGAATTCCTTTCATACTA+TGG | + | 57554:5684-5703 | MS.gene047883:intron | 25.0% |
| ! | CTAAGTGTGCAAATAAAAAA+TGG | - | 57554:5713-5732 | MS.gene047883:intergenic | 25.0% |
| ! | GTTATGGTTATGTTATGTTA+CGG | + | 57554:5113-5132 | MS.gene047883:intron | 25.0% |
| ! | TTAGATAAGGATAGAAACAT+TGG | + | 57554:4723-4742 | MS.gene047883:intron | 25.0% |
| ! | TTCTATAGAAAGCAAAAGAA+AGG | - | 57554:5890-5909 | MS.gene047883:intergenic | 25.0% |
| !! | GCAATACTTATTCTAGTTAT+TGG | + | 57554:5006-5025 | MS.gene047883:intron | 25.0% |
| !! | GTATTGAGACATGTAATTTT+TGG | + | 57554:5091-5110 | MS.gene047883:intron | 25.0% |
| !!! | ACTTTATTAAGAAGTATCAG+AGG | + | 57554:5660-5679 | MS.gene047883:CDS | 25.0% |
| !!! | AGACATGTAATTTTTGGTTA+TGG | + | 57554:5097-5116 | MS.gene047883:intron | 25.0% |
| !!! | ATTTTAGTCCTCAAATGTAT+TGG | + | 57554:4546-4565 | MS.gene047883:intron | 25.0% |
| !!! | TTCTGTTTCTAATACAGAAA+TGG | + | 57554:5494-5513 | MS.gene047883:intron | 25.0% |
| ACACTTGCAAGACATAATAT+AGG | - | 57554:5186-5205 | MS.gene047883:intergenic | 30.0% | |
| ACAGAGGAGTAACAAAAAAA+GGG | - | 57554:5753-5772 | MS.gene047883:intergenic | 30.0% | |
| ACATACCATAGAAACACTTT+GGG | - | 57554:4230-4249 | MS.gene047883:intergenic | 30.0% | |
| ATGCAAAGCACAATTAGATA+AGG | + | 57554:4710-4729 | MS.gene047883:intron | 30.0% | |
| ATGTTATTATTGGACAATGC+AGG | + | 57554:5845-5864 | MS.gene047883:CDS | 30.0% | |
| TACATACCATAGAAACACTT+TGG | - | 57554:4231-4250 | MS.gene047883:intergenic | 30.0% | |
| TAGAGTAGTTGCACAAATAA+AGG | - | 57554:5967-5986 | MS.gene047883:intergenic | 30.0% | |
| TATAGACCAATTCTTTCAAG+CGG | - | 57554:5142-5161 | MS.gene047883:intergenic | 30.0% | |
| TCCTCAAATGTATTGGATTT+GGG | + | 57554:4553-4572 | MS.gene047883:intron | 30.0% | |
| TGAATAGGACTATTATTGTC+AGG | + | 57554:5624-5643 | MS.gene047883:CDS | 30.0% | |
| TGCATTGTCCAATAATAACA+TGG | - | 57554:5846-5865 | MS.gene047883:intergenic | 30.0% | |
| TTTATTTGTGCAACTACTCT+AGG | + | 57554:5966-5985 | MS.gene047883:intron | 30.0% | |
| ! | ATTGTTAATAGCTCTGTGTA+TGG | + | 57554:5300-5319 | MS.gene047883:intron | 30.0% |
| AAGATACAGAGATAGAACGA+AGG | - | 57554:4141-4160 | MS.gene047882:intergenic | 35.0% | |
| AGTTCGAAGAAATCTGGTAA+GGG | + | 57554:4336-4355 | MS.gene047883:intron | 35.0% | |
| ATAGGACTATTATTGTCAGG+AGG | + | 57554:5627-5646 | MS.gene047883:CDS | 35.0% | |
| CCTCAAATGTATTGGATTTG+GGG | + | 57554:4554-4573 | MS.gene047883:intron | 35.0% | |
| GAAGGAGACCATGTTATTAT+TGG | + | 57554:5835-5854 | MS.gene047883:CDS | 35.0% | |
| GACAGAGGAGTAACAAAAAA+AGG | - | 57554:5754-5773 | MS.gene047883:intergenic | 35.0% | |
| GTCCTCAAATGTATTGGATT+TGG | + | 57554:4552-4571 | MS.gene047883:intron | 35.0% | |
| TCGTTCTATCTCTGTATCTT+TGG | + | 57554:4141-4160 | MS.gene047883:intron | 35.0% | |
| TTCTTTGTTACAGACTGAGA+AGG | + | 57554:4190-4209 | MS.gene047883:intron | 35.0% | |
| ! | AATAGCTCTGTGTATGGTTA+CGG | + | 57554:5306-5325 | MS.gene047883:intron | 35.0% |
| ! | AATCGTTTCTGGAAATCAGT+TGG | + | 57554:4381-4400 | MS.gene047883:CDS | 35.0% |
| ! | TATGTCTTGCAAGTGTTGTT+GGG | + | 57554:5190-5209 | MS.gene047883:intron | 35.0% |
| ! | TGTAGGGATAAACAGCATTT+TGG | + | 57554:4495-4514 | MS.gene047883:intron | 35.0% |
| ! | TTATGTCTTGCAAGTGTTGT+TGG | + | 57554:5189-5208 | MS.gene047883:intron | 35.0% |
| ! | TTTTTGTTACTCCTCTGTCT+AGG | + | 57554:5755-5774 | MS.gene047883:intron | 35.0% |
| !!! | ACGCTATTTCTTTTGCATAG+TGG | + | 57554:4762-4781 | MS.gene047883:intron | 35.0% |
| AATGGGGACCATAGTATGAA+AGG | - | 57554:5695-5714 | MS.gene047883:intergenic | 40.0% | |
| AGATTCCTCTTGGCAATATC+AGG | + | 57554:4863-4882 | MS.gene047883:intron | 40.0% | |
| CAATGCAGGTGAGACTTTAT+AGG | + | 57554:5859-5878 | MS.gene047883:intron | 40.0% | |
| CAGTTCGAAGAAATCTGGTA+AGG | + | 57554:4335-4354 | MS.gene047883:intron | 40.0% | |
| CCCCAAATCCAATACATTTG+AGG | - | 57554:4557-4576 | MS.gene047883:intergenic | 40.0% | |
| GAAAAGGTGGAAATCGTTTC+TGG | + | 57554:4370-4389 | MS.gene047883:CDS | 40.0% | |
| GTTCGAAGAAATCTGGTAAG+GGG | + | 57554:4337-4356 | MS.gene047883:intron | 40.0% | |
| TAATAGAGCCTCACATTCGA+GGG | + | 57554:4588-4607 | MS.gene047883:intron | 40.0% | |
| TCCTATTCATCTTAGCACTG+TGG | - | 57554:5613-5632 | MS.gene047883:intergenic | 40.0% | |
| TGGACACTTCTTGTCAATGT+AGG | - | 57554:5543-5562 | MS.gene047883:intergenic | 40.0% | |
| TGTTGCAGTTCGAAGAAATC+TGG | + | 57554:4330-4349 | MS.gene047883:intron | 40.0% | |
| ! | AAACGATTTCCACCTTTTCC+AGG | - | 57554:4369-4388 | MS.gene047883:intergenic | 40.0% |
| ! | AGCAACCCAAAGTGTTTCTA+TGG | + | 57554:4222-4241 | MS.gene047883:CDS | 40.0% |
| ! | TGTTTGTCTGAGATTCCTCT+TGG | + | 57554:4853-4872 | MS.gene047883:intron | 40.0% |
| ! | TTCTGGAAATCAGTTGGTCT+TGG | + | 57554:4387-4406 | MS.gene047883:CDS | 40.0% |
| AGAAATCTGGTAAGGGGAAG+AGG | + | 57554:4343-4362 | MS.gene047883:CDS | 45.0% | |
| ATCACCTTGCTTCCGTGTTA+AGG | + | 57554:5813-5832 | MS.gene047883:CDS | 45.0% | |
| ATCTAGTGGTGCAGGAAAGA+AGG | + | 57554:6042-6061 | MS.gene047883:CDS | 45.0% | |
| ATGGAAACATTGCCGGTGAA+TGG | - | 57554:5563-5582 | MS.gene047883:intergenic | 45.0% | |
| ATTACCTTCAGTGGCTTCTC+TGG | - | 57554:4424-4443 | MS.gene047883:intergenic | 45.0% | |
| CATGGTCTCCTTCCTTAACA+CGG | - | 57554:5828-5847 | MS.gene047883:intergenic | 45.0% | |
| CGGTATCCGCTTGAAAGAAT+TGG | + | 57554:5133-5152 | MS.gene047883:intron | 45.0% | |
| CTCTGTCTAGGTATGAGAAG+AGG | + | 57554:5767-5786 | MS.gene047883:intron | 45.0% | |
| CTCTTCTCATACCTAGACAG+AGG | - | 57554:5769-5788 | MS.gene047883:intergenic | 45.0% | |
| GCCACAGTGCTAAGATGAAT+AGG | + | 57554:5609-5628 | MS.gene047883:CDS | 45.0% | |
| GTAATAGAGCCTCACATTCG+AGG | + | 57554:4587-4606 | MS.gene047883:intron | 45.0% | |
| TTACCTTCAGTGGCTTCTCT+GGG | - | 57554:4423-4442 | MS.gene047883:intergenic | 45.0% | |
| TTCCTCTTGGCAATATCAGG+TGG | + | 57554:4866-4885 | MS.gene047883:intron | 45.0% | |
| ! | GCAGGAAAGAAGGCTTTTAC+TGG | + | 57554:6052-6071 | MS.gene047883:CDS | 45.0% |
| ! | TCTTGCAAGTGTTGTTGGGA+AGG | + | 57554:5194-5213 | MS.gene047883:intron | 45.0% |
| !! | CTGAGCGTCTAGCTTTGTAT+TGG | + | 57554:4828-4847 | MS.gene047883:intron | 45.0% |
| !! | GACAAGAAGTGTCCATTCAC+CGG | + | 57554:5548-5567 | MS.gene047883:CDS | 45.0% |
| AACCTCACAGTCTTGGAGAG+TGG | - | 57554:5992-6011 | MS.gene047882:intergenic | 50.0% | |
| AAGCAAGGTGATACGTGAGC+GGG | - | 57554:5805-5824 | MS.gene047882:intergenic | 50.0% | |
| AGCCACCTGATATTGCCAAG+AGG | - | 57554:4871-4890 | MS.gene047883:intergenic | 50.0% | |
| ATCCCAGCTGGATCATCTAG+TGG | + | 57554:6028-6047 | MS.gene047883:CDS | 50.0% | |
| CACCACTAGATGATCCAGCT+GGG | - | 57554:6033-6052 | MS.gene047883:intergenic | 50.0% | |
| CACGTTGAACCTCACAGTCT+TGG | - | 57554:5999-6018 | MS.gene047883:intergenic | 50.0% | |
| CCTTCCTTAACACGGAAGCA+AGG | - | 57554:5820-5839 | MS.gene047883:intergenic | 50.0% | |
| CCTTGCTTCCGTGTTAAGGA+AGG | + | 57554:5817-5836 | MS.gene047883:CDS | 50.0% | |
| CTTAGCACTGTGGCAAGTTC+CGG | - | 57554:5603-5622 | MS.gene047883:intergenic | 50.0% | |
| GAAGCAAGGTGATACGTGAG+CGG | - | 57554:5806-5825 | MS.gene047883:intergenic | 50.0% | |
| GTGTTGAAAGTGATCCCAGC+TGG | + | 57554:6016-6035 | MS.gene047883:CDS | 50.0% | |
| GTTATGCTCCCTCGAATGTG+AGG | - | 57554:4599-4618 | MS.gene047883:intergenic | 50.0% | |
| TACCTTCAGTGGCTTCTCTG+GGG | - | 57554:4422-4441 | MS.gene047883:intergenic | 50.0% | |
| TCACCGGCAATGTTTCCATC+CGG | + | 57554:5564-5583 | MS.gene047883:CDS | 50.0% | |
| AAGGGGAAGAGGCCTGGAAA+AGG | + | 57554:4354-4373 | MS.gene047883:CDS | 55.0% | |
| ACCCCGGATGGAAACATTGC+CGG | - | 57554:5570-5589 | MS.gene047883:intergenic | 55.0% | |
| ACCGGCAATGTTTCCATCCG+GGG | + | 57554:5566-5585 | MS.gene047883:CDS | 55.0% | |
| ACCTTCAGTGGCTTCTCTGG+GGG | - | 57554:4421-4440 | MS.gene047883:intergenic | 55.0% | |
| ATCCGGGGTCGTATCTTAGC+CGG | + | 57554:5581-5600 | MS.gene047883:CDS | 55.0% | |
| CACCGGCAATGTTTCCATCC+GGG | + | 57554:5565-5584 | MS.gene047883:CDS | 55.0% | |
| GCACCACTAGATGATCCAGC+TGG | - | 57554:6034-6053 | MS.gene047883:intergenic | 55.0% | |
| GCTGGATCATCTAGTGGTGC+AGG | + | 57554:6034-6053 | MS.gene047883:CDS | 55.0% | |
| TTCCGGCTAAGATACGACCC+CGG | - | 57554:5586-5605 | MS.gene047883:intergenic | 55.0% | |
| ACCCCCAGAGAAGCCACTGA+AGG | + | 57554:4417-4436 | MS.gene047883:CDS | 60.0% | |
| GGCCACTCTCCAAGACTGTG+AGG | + | 57554:5987-6006 | MS.gene047883:intron | 60.0% | |
| GGCTAAGATACGACCCCGGA+TGG | - | 57554:5582-5601 | MS.gene047883:intergenic | 60.0% | |
| GGGAAGAGGCCTGGAAAAGG+TGG | + | 57554:4357-4376 | MS.gene047883:CDS | 60.0% | |
| TCTGGTAAGGGGAAGAGGCC+TGG | + | 57554:4348-4367 | MS.gene047883:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57554 | gene | 4106 | 6081 | 4106 | ID=MS.gene047883 |
| 57554 | mRNA | 4106 | 6081 | 4106 | ID=MS.gene047883.t1;Parent=MS.gene047883 |
| 57554 | exon | 4106 | 4117 | 4106 | ID=MS.gene047883.t1.exon1;Parent=MS.gene047883.t1 |
| 57554 | CDS | 4106 | 4117 | 4106 | ID=cds.MS.gene047883.t1;Parent=MS.gene047883.t1 |
| 57554 | exon | 4203 | 4243 | 4203 | ID=MS.gene047883.t1.exon2;Parent=MS.gene047883.t1 |
| 57554 | CDS | 4203 | 4243 | 4203 | ID=cds.MS.gene047883.t1;Parent=MS.gene047883.t1 |
| 57554 | exon | 4338 | 4438 | 4338 | ID=MS.gene047883.t1.exon3;Parent=MS.gene047883.t1 |
| 57554 | CDS | 4338 | 4438 | 4338 | ID=cds.MS.gene047883.t1;Parent=MS.gene047883.t1 |
| 57554 | exon | 5537 | 5681 | 5537 | ID=MS.gene047883.t1.exon4;Parent=MS.gene047883.t1 |
| 57554 | CDS | 5537 | 5681 | 5537 | ID=cds.MS.gene047883.t1;Parent=MS.gene047883.t1 |
| 57554 | exon | 5777 | 5866 | 5777 | ID=MS.gene047883.t1.exon5;Parent=MS.gene047883.t1 |
| 57554 | CDS | 5777 | 5866 | 5777 | ID=cds.MS.gene047883.t1;Parent=MS.gene047883.t1 |
| 57554 | exon | 5988 | 6081 | 5988 | ID=MS.gene047883.t1.exon6;Parent=MS.gene047883.t1 |
| 57554 | CDS | 5988 | 6081 | 5988 | ID=cds.MS.gene047883.t1;Parent=MS.gene047883.t1 |
| Gene Sequence |
| Protein sequence |
