AlfalfaGEDB | MS.gene25778

M. sativa cultivar XinJiangDaYe / MS.gene25778

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14223.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25778.t1	GAU34576.1	72.4	156	39	2	1	155	150	302	1.20E-52	216.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25778.t1	A0A2Z6MQL6	72.4	156	39	2	1	155	150	302	8.4e-53	216.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene25778	0.801564	8.48E-49	-1.69E-46
MS.gene049254	MS.gene25778	0.828695	7.79E-55	-1.69E-46
MS.gene049404	MS.gene25778	0.802371	5.79E-49	-1.69E-46
MS.gene049435	MS.gene25778	0.813669	2.28E-51	-1.69E-46
MS.gene049587	MS.gene25778	0.803262	3.79E-49	-1.69E-46
MS.gene049666	MS.gene25778	0.805544	1.27E-49	-1.69E-46
MS.gene050026	MS.gene25778	0.814076	1.85E-51	-1.69E-46
MS.gene050156	MS.gene25778	0.811436	7.01E-51	-1.69E-46
MS.gene050355	MS.gene25778	0.819926	8.99E-53	-1.69E-46
MS.gene050430	MS.gene25778	0.810322	1.22E-50	-1.69E-46
MS.gene050442	MS.gene25778	0.817695	2.89E-52	-1.69E-46
MS.gene050611	MS.gene25778	0.815241	1.02E-51	-1.69E-46
MS.gene050750	MS.gene25778	0.812951	3.27E-51	-1.69E-46
MS.gene050844	MS.gene25778	0.801979	6.97E-49	-1.69E-46
MS.gene050953	MS.gene25778	0.804956	1.68E-49	-1.69E-46
MS.gene051265	MS.gene25778	0.812912	3.34E-51	-1.69E-46
MS.gene051279	MS.gene25778	0.81167	6.23E-51	-1.69E-46
MS.gene051594	MS.gene25778	0.825948	3.55E-54	-1.69E-46
MS.gene051710	MS.gene25778	0.802706	4.94E-49	-1.69E-46
MS.gene052190	MS.gene25778	0.833693	4.61E-56	-1.69E-46
MS.gene052333	MS.gene25778	0.837698	4.46E-57	-1.69E-46
MS.gene052488	MS.gene25778	0.805852	1.09E-49	-1.69E-46
MS.gene052519	MS.gene25778	0.833514	5.10E-56	-1.69E-46
MS.gene052582	MS.gene25778	0.83478	2.46E-56	-1.69E-46
MS.gene052584	MS.gene25778	0.840093	1.07E-57	-1.69E-46
MS.gene052611	MS.gene25778	0.802187	6.32E-49	-1.69E-46
MS.gene052773	MS.gene25778	0.80728	5.46E-50	-1.69E-46
MS.gene052829	MS.gene25778	0.811848	5.70E-51	-1.69E-46
MS.gene052843	MS.gene25778	0.821557	3.79E-53	-1.69E-46
MS.gene052863	MS.gene25778	0.80796	3.92E-50	-1.69E-46
MS.gene052867	MS.gene25778	0.830043	3.67E-55	-1.69E-46
MS.gene053145	MS.gene25778	0.817332	3.48E-52	-1.69E-46
MS.gene053166	MS.gene25778	0.815778	7.77E-52	-1.69E-46
MS.gene053202	MS.gene25778	0.804688	1.92E-49	-1.69E-46
MS.gene053226	MS.gene25778	0.822834	1.92E-53	-1.69E-46
MS.gene053883	MS.gene25778	0.800217	1.60E-48	-1.69E-46
MS.gene053953	MS.gene25778	0.805027	1.63E-49	-1.69E-46
MS.gene053966	MS.gene25778	0.802505	5.44E-49	-1.69E-46
MS.gene053967	MS.gene25778	0.8108	9.63E-51	-1.69E-46
MS.gene053974	MS.gene25778	0.824797	6.64E-54	-1.69E-46
MS.gene053998	MS.gene25778	0.83044	2.93E-55	-1.69E-46
MS.gene05414	MS.gene25778	0.812384	4.36E-51	-1.69E-46
MS.gene05415	MS.gene25778	0.819428	1.17E-52	-1.69E-46
MS.gene054236	MS.gene25778	0.837	6.73E-57	-1.69E-46
MS.gene054291	MS.gene25778	-0.836927	7.02E-57	-1.69E-46
MS.gene054405	MS.gene25778	0.804446	2.15E-49	-1.69E-46
MS.gene054467	MS.gene25778	0.817137	3.85E-52	-1.69E-46
MS.gene054559	MS.gene25778	0.826923	2.08E-54	-1.69E-46
MS.gene054651	MS.gene25778	0.841354	5.00E-58	-1.69E-46
MS.gene054775	MS.gene25778	0.814445	1.53E-51	-1.69E-46
MS.gene054801	MS.gene25778	0.804835	1.78E-49	-1.69E-46
MS.gene055166	MS.gene25778	0.807127	5.88E-50	-1.69E-46
MS.gene055227	MS.gene25778	0.819077	1.40E-52	-1.69E-46
MS.gene055228	MS.gene25778	0.833526	5.07E-56	-1.69E-46
MS.gene055356	MS.gene25778	0.818443	1.96E-52	-1.69E-46
MS.gene055357	MS.gene25778	0.838568	2.66E-57	-1.69E-46
MS.gene055586	MS.gene25778	0.803448	3.47E-49	-1.69E-46
MS.gene055589	MS.gene25778	0.811253	7.68E-51	-1.69E-46
MS.gene055818	MS.gene25778	0.840853	6.77E-58	-1.69E-46
MS.gene05604	MS.gene25778	0.815169	1.06E-51	-1.69E-46
MS.gene056050	MS.gene25778	0.804054	2.60E-49	-1.69E-46
MS.gene056162	MS.gene25778	0.815286	9.99E-52	-1.69E-46
MS.gene056173	MS.gene25778	0.811806	5.82E-51	-1.69E-46
MS.gene056174	MS.gene25778	0.810479	1.13E-50	-1.69E-46
MS.gene056252	MS.gene25778	0.83334	5.64E-56	-1.69E-46
MS.gene056366	MS.gene25778	0.818936	1.51E-52	-1.69E-46
MS.gene056498	MS.gene25778	0.825597	4.30E-54	-1.69E-46
MS.gene056506	MS.gene25778	0.806077	9.80E-50	-1.69E-46
MS.gene056595	MS.gene25778	0.805835	1.10E-49	-1.69E-46
MS.gene057021	MS.gene25778	0.81996	8.83E-53	-1.69E-46
MS.gene057060	MS.gene25778	0.82517	5.42E-54	-1.69E-46
MS.gene057314	MS.gene25778	0.825062	5.75E-54	-1.69E-46
MS.gene057444	MS.gene25778	0.832072	1.16E-55	-1.69E-46
MS.gene058010	MS.gene25778	0.807318	5.36E-50	-1.69E-46
MS.gene058037	MS.gene25778	0.831133	1.98E-55	-1.69E-46
MS.gene058294	MS.gene25778	0.815022	1.14E-51	-1.69E-46
MS.gene058515	MS.gene25778	0.820249	7.58E-53	-1.69E-46
MS.gene058625	MS.gene25778	0.829895	3.99E-55	-1.69E-46
MS.gene058829	MS.gene25778	0.812255	4.65E-51	-1.69E-46
MS.gene058979	MS.gene25778	0.830716	2.51E-55	-1.69E-46
MS.gene059272	MS.gene25778	0.81877	1.65E-52	-1.69E-46
MS.gene059584	MS.gene25778	0.837762	4.29E-57	-1.69E-46
MS.gene059601	MS.gene25778	0.803752	3.00E-49	-1.69E-46
MS.gene059625	MS.gene25778	0.802178	6.35E-49	-1.69E-46
MS.gene060011	MS.gene25778	0.817169	3.79E-52	-1.69E-46
MS.gene060137	MS.gene25778	-0.807998	3.84E-50	-1.69E-46
MS.gene060167	MS.gene25778	0.801911	7.20E-49	-1.69E-46
MS.gene060322	MS.gene25778	0.829065	6.34E-55	-1.69E-46
MS.gene060521	MS.gene25778	0.810823	9.52E-51	-1.69E-46
MS.gene060781	MS.gene25778	0.807943	3.95E-50	-1.69E-46
MS.gene061091	MS.gene25778	0.805008	1.64E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25778.t1	MTR_1g021965	60.377	53	21	0	18	70	112	164	1.89e-15	70.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 27 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTGAGTCAAATTTGCTTT+TGG	0.290903	3.2:+70850602	MS.gene25778:CDS
AAGCATATCACTGCAGATAT+TGG	0.320627	3.2:+70850559	MS.gene25778:CDS
TTCTCTCGACTCCAAAGCTT+TGG	0.344968	3.2:-70850406	None:intergenic
GTTGTTTGCGTGTATTGTTT+TGG	0.356104	3.2:-70850437	None:intergenic
AGCTGCACGAGCCAAAGCTT+TGG	0.379774	3.2:+70850395	MS.gene25778:CDS
GCGGAGAACATCATACTTCC+AGG	0.389492	3.2:+70850225	MS.gene25778:CDS
TCTCCGCCTTCAATTCTTCA+AGG	0.400192	3.2:-70850209	None:intergenic
CGTGTTTGTCGCTGATGCCT+TGG	0.446247	3.2:+70850248	MS.gene25778:CDS
CCTTCAATTCTTCAAGGAGT+TGG	0.448637	3.2:-70850203	None:intergenic
CACACACATAGCAACAATCT+AGG	0.462178	3.2:-70850521	None:intergenic
CCAACTCCTTGAAGAATTGA+AGG	0.465521	3.2:+70850203	MS.gene25778:CDS
CTAGGTTCGATTTAAAATTG+AGG	0.476408	3.2:-70850503	None:intergenic
TTGTAATCATTCCATGAGTC+GGG	0.479069	3.2:-70850282	None:intergenic
TTGAAAAGTTGCCCGACTCA+TGG	0.500599	3.2:+70850271	MS.gene25778:CDS
CTTGTAATCATTCCATGAGT+CGG	0.537899	3.2:-70850283	None:intergenic
TGCTATGTGTGTGGAAAGAA+AGG	0.547994	3.2:+70850531	MS.gene25778:CDS
GCCAAAAGCAAATTTGACTC+AGG	0.550779	3.2:-70850603	None:intergenic
TCTGTAATGGCAAATGAAAG+AGG	0.555816	3.2:+70850146	None:intergenic
GCATCAGCGACAAACACGCC+TGG	0.558250	3.2:-70850243	None:intergenic
ATTGAGGTTAGTTACTCTGA+GGG	0.569491	3.2:-70850487	None:intergenic
AATTGAGGTTAGTTACTCTG+AGG	0.613266	3.2:-70850488	None:intergenic
CTAGATTGTTGCTATGTGTG+TGG	0.632111	3.2:+70850522	MS.gene25778:CDS
ATGGCAAATGAAAGAGGACA+AGG	0.655713	3.2:+70850152	None:intergenic
ACAATACACGCAAACAACAG+AGG	0.686335	3.2:+70850442	MS.gene25778:CDS
GACACCAGCAGAAAAGAATG+CGG	0.696693	3.2:+70850372	MS.gene25778:CDS
AGGACAAGGAAATCAAAGTG+CGG	0.706319	3.2:+70850166	MS.gene25778:CDS
ACTCCTTGAAGAATTGAAGG+CGG	0.711654	3.2:+70850206	MS.gene25778:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTAGGTTCGATTTAAAATTG+AGG	-	chr3.2:70850506-70850525	None:intergenic	30.0%
	AAGCATATCACTGCAGATAT+TGG	+	chr3.2:70850559-70850578	MS.gene25778:CDS	35.0%
	CTTGTAATCATTCCATGAGT+CGG	-	chr3.2:70850286-70850305	None:intergenic	35.0%
	TTGTAATCATTCCATGAGTC+GGG	-	chr3.2:70850285-70850304	None:intergenic	35.0%
!	AATTGAGGTTAGTTACTCTG+AGG	-	chr3.2:70850491-70850510	None:intergenic	35.0%
!	ATTGAGGTTAGTTACTCTGA+GGG	-	chr3.2:70850490-70850509	None:intergenic	35.0%
!	GTTGTTTGCGTGTATTGTTT+TGG	-	chr3.2:70850440-70850459	None:intergenic	35.0%
!!	ACCTGAGTCAAATTTGCTTT+TGG	+	chr3.2:70850602-70850621	MS.gene25778:CDS	35.0%
	ACAATACACGCAAACAACAG+AGG	+	chr3.2:70850442-70850461	MS.gene25778:CDS	40.0%
	ACTCCTTGAAGAATTGAAGG+CGG	+	chr3.2:70850206-70850225	MS.gene25778:CDS	40.0%
	AGGACAAGGAAATCAAAGTG+CGG	+	chr3.2:70850166-70850185	MS.gene25778:CDS	40.0%
	CACACACATAGCAACAATCT+AGG	-	chr3.2:70850524-70850543	None:intergenic	40.0%
	CCAACTCCTTGAAGAATTGA+AGG	+	chr3.2:70850203-70850222	MS.gene25778:CDS	40.0%
	CCTTCAATTCTTCAAGGAGT+TGG	-	chr3.2:70850206-70850225	None:intergenic	40.0%
	CTAGATTGTTGCTATGTGTG+TGG	+	chr3.2:70850522-70850541	MS.gene25778:CDS	40.0%
	GCCAAAAGCAAATTTGACTC+AGG	-	chr3.2:70850606-70850625	None:intergenic	40.0%
	TGCTATGTGTGTGGAAAGAA+AGG	+	chr3.2:70850531-70850550	MS.gene25778:CDS	40.0%
	GACACCAGCAGAAAAGAATG+CGG	+	chr3.2:70850372-70850391	MS.gene25778:CDS	45.0%
	TCTCCGCCTTCAATTCTTCA+AGG	-	chr3.2:70850212-70850231	None:intergenic	45.0%
	TTCTCTCGACTCCAAAGCTT+TGG	-	chr3.2:70850409-70850428	None:intergenic	45.0%
	TTGAAAAGTTGCCCGACTCA+TGG	+	chr3.2:70850271-70850290	MS.gene25778:CDS	45.0%
	GCGGAGAACATCATACTTCC+AGG	+	chr3.2:70850225-70850244	MS.gene25778:CDS	50.0%
!	AGCTCCGCATTCTTTTCTGC+TGG	-	chr3.2:70850379-70850398	None:intergenic	50.0%
!	GTCGGGCAACTTTTCAACCA+AGG	-	chr3.2:70850268-70850287	None:intergenic	50.0%
	AGCTGCACGAGCCAAAGCTT+TGG	+	chr3.2:70850395-70850414	MS.gene25778:CDS	55.0%
	CGTGTTTGTCGCTGATGCCT+TGG	+	chr3.2:70850248-70850267	MS.gene25778:CDS	55.0%
	GCATCAGCGACAAACACGCC+TGG	-	chr3.2:70850246-70850265	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	70850159	70850629	70850159	ID=MS.gene25778
chr3.2	mRNA	70850159	70850629	70850159	ID=MS.gene25778.t1;Parent=MS.gene25778
chr3.2	exon	70850159	70850629	70850159	ID=MS.gene25778.t1.exon1;Parent=MS.gene25778.t1
chr3.2	CDS	70850159	70850629	70850159	ID=cds.MS.gene25778.t1;Parent=MS.gene25778.t1

Gene Sequence

>MS.gene25778

ATGAAAGAGGACAAGGAAATCAAAGTGCGGATCAATGAGTATCACCAACTCCTTGAAGAATTGAAGGCGGAGAACATCATACTTCCAGGCGTGTTTGTCGCTGATGCCTTGGTTGAAAAGTTGCCCGACTCATGGAATGATTACAAGCAACAGCTGAAACATAAGCACAAGCAGATGTTTCTTAATGATCTCATCAAACACATAATCATTGAAGACACCAGCAGAAAAGAATGCGGAGCTGCACGAGCCAAAGCTTTGGAGTCGAGAGAAAATCTAATCCAAAACAATACACGCAAACAACAGAGGTATGAAAATAAAAATGATTATGCCCTCAGAGTAACTAACCTCAATTTTAAATCGAACCTAGATTGTTGCTATGTGTGTGGAAAGAAAGGTCATAAAGCATATCACTGCAGATATTGGAAAGTGAATCGAACATTCGAACCTGAGTCAAATTTGCTTTTGGCTTAG

Protein sequence

>MS.gene25778.t1

MKEDKEIKVRINEYHQLLEELKAENIILPGVFVADALVEKLPDSWNDYKQQLKHKHKQMFLNDLIKHIIIEDTSRKECGAARAKALESRENLIQNNTRKQQRYENKNDYALRVTNLNFKSNLDCCYVCGKKGHKAYHCRYWKVNRTFEPESNLLLA