AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59840.t1 MTR_8g045400 92.258 155 11 1 1 155 1 154 2.34e-104 295
MS.gene59840.t1 MTR_8g045490 78.289 152 33 0 1 152 1 152 2.06e-90 260
MS.gene59840.t1 MTR_8g045520 79.355 155 32 0 1 155 1 155 4.03e-90 259
MS.gene59840.t1 MTR_8g045695 74.839 155 38 1 1 155 1 154 2.11e-85 247
MS.gene59840.t1 MTR_8g045735 72.258 155 42 1 1 155 1 154 8.98e-82 238
MS.gene59840.t1 MTR_8g045570 71.053 152 44 0 1 152 1 152 1.91e-81 237
MS.gene59840.t1 MTR_8g045640 74.194 155 39 1 1 155 1 154 2.49e-81 237
MS.gene59840.t1 MTR_8g045665 72.903 155 41 1 1 155 1 154 3.34e-81 236
MS.gene59840.t1 MTR_8g045560 72.903 155 41 1 1 155 1 154 1.53e-80 235
MS.gene59840.t1 MTR_8g045300 69.677 155 47 0 1 155 1 155 7.28e-80 233
MS.gene59840.t1 MTR_8g045555 70.395 152 45 0 1 152 1 152 8.28e-80 233
MS.gene59840.t1 MTR_1g030820 38.562 153 91 2 1 151 1 152 3.49e-38 127
MS.gene59840.t1 MTR_1g030810 38.562 153 91 2 1 151 1 152 2.73e-37 125
MS.gene59840.t1 MTR_1g030840 36.943 157 89 5 1 149 1 155 3.98e-29 105
MS.gene59840.t1 MTR_8g012550 34.483 145 92 3 6 149 8 150 1.20e-26 98.6
MS.gene59840.t1 MTR_1g031640 37.162 148 84 5 7 152 7 147 3.26e-24 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59840.t1 AT5G28010 35.333 150 94 3 3 150 15 163 1.21e-27 101
MS.gene59840.t1 AT5G28010 35.333 150 94 3 3 150 15 163 1.21e-27 101
MS.gene59840.t1 AT1G70890 37.333 150 91 3 3 150 7 155 2.56e-26 97.8
MS.gene59840.t1 AT1G70840 35.333 150 94 3 3 150 20 168 6.13e-26 97.1
MS.gene59840.t1 AT1G70830 36.667 150 92 3 3 150 22 170 1.45e-25 96.3
MS.gene59840.t1 AT1G70830 36.667 150 92 3 3 150 22 170 2.63e-25 96.3
MS.gene59840.t1 AT1G70830 36.667 150 92 3 3 150 22 170 1.13e-24 95.9
MS.gene59840.t1 AT1G70830 36.667 150 92 3 3 150 22 170 3.18e-24 96.3
MS.gene59840.t1 AT1G70830 35.333 150 94 3 3 150 184 332 1.03e-22 92.0
MS.gene59840.t1 AT5G28000 34.000 150 96 3 3 150 13 161 3.39e-24 92.4
MS.gene59840.t1 AT1G70850 34.667 150 95 3 3 150 9 157 2.87e-23 91.7
MS.gene59840.t1 AT1G70830 35.333 150 94 3 3 150 137 285 3.12e-23 92.8
MS.gene59840.t1 AT1G70830 33.333 93 61 1 59 150 31 123 6.13e-13 65.1
MS.gene59840.t1 AT1G70850 34.667 150 95 3 3 150 9 157 1.03e-22 92.0
MS.gene59840.t1 AT1G70850 35.714 140 87 3 13 150 175 313 1.51e-21 88.6
MS.gene59840.t1 AT1G70850 34.667 150 95 3 3 150 9 157 1.03e-22 92.0
MS.gene59840.t1 AT1G70850 35.714 140 87 3 13 150 175 313 1.51e-21 88.6
MS.gene59840.t1 AT1G23130 28.859 149 104 2 3 150 10 157 1.48e-20 82.8
MS.gene59840.t1 AT1G23120 28.000 150 103 3 1 150 1 145 1.25e-19 80.1
MS.gene59840.t1 AT1G70870 40.625 96 56 1 56 151 44 138 1.30e-19 80.1
MS.gene59840.t1 AT1G70880 30.667 150 101 3 3 150 8 156 1.44e-19 80.5
MS.gene59840.t1 AT4G23670 32.680 153 100 3 1 152 1 151 1.45e-19 80.1
MS.gene59840.t1 AT1G23120 28.472 144 98 3 7 150 57 195 3.44e-19 80.5
MS.gene59840.t1 AT2G01520 29.412 153 105 3 1 152 1 151 1.23e-17 75.1
MS.gene59840.t1 AT1G14930 27.742 155 109 3 1 154 1 153 2.72e-17 74.3
MS.gene59840.t1 AT1G35260 28.767 146 100 3 7 150 6 149 1.08e-16 72.8
MS.gene59840.t1 AT2G01530 30.065 153 104 3 1 152 1 151 1.15e-16 72.8
MS.gene59840.t1 AT4G14060 29.412 153 105 3 1 152 1 151 1.88e-16 72.0
MS.gene59840.t1 AT4G23680 28.289 152 106 3 1 151 1 150 9.08e-16 70.1
MS.gene59840.t1 AT1G14940 27.097 155 110 3 1 154 1 153 1.08e-14 67.4
MS.gene59840.t1 AT1G30990 29.801 151 102 4 1 150 1 148 1.22e-14 67.4
MS.gene59840.t1 AT1G14950 27.742 155 109 3 1 154 1 153 1.89e-14 67.0
MS.gene59840.t1 AT3G26450 29.221 154 105 4 1 152 1 152 3.49e-14 66.2
MS.gene59840.t1 AT1G24020 29.677 155 107 2 1 154 1 154 4.30e-14 65.9
MS.gene59840.t1 AT1G24020 29.677 155 107 2 1 154 1 154 4.30e-14 65.9
MS.gene59840.t1 AT1G35310 25.899 139 100 3 13 150 12 148 1.23e-13 64.7
MS.gene59840.t1 AT1G14960 27.632 152 107 3 1 151 1 150 5.19e-12 60.5
MS.gene59840.t1 AT3G26460 29.487 156 102 5 1 152 1 152 1.08e-11 59.7

Find 28 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCACCTTCATGAAGTT+TGG 0.292428 8.3:-54101922 None:intergenic
CATTCAGAAATGGTGCTTGC+TGG 0.350620 8.3:+54101792 None:intergenic
AATGGCTGGATGGACTTATT+AGG 0.352875 8.3:+54102957 MS.gene59840:CDS
AAAATAACTTTCAAGCTCTT+CGG 0.428417 8.3:+54102807 MS.gene59840:CDS
ATCATTGAAGTAATTGATAA+TGG 0.436398 8.3:+54102867 MS.gene59840:CDS
ATGGCAGTGGAGCTGCAAAA+TGG 0.452179 8.3:+54102892 MS.gene59840:CDS
AATAAGTCCATCCAGCCATT+TGG 0.456616 8.3:-54102954 None:intergenic
AACTTCATGAAGGTGAAGAT+TGG 0.494334 8.3:+54101925 MS.gene59840:CDS
AGACATTGATGAGCACTATA+AGG 0.495099 8.3:+54102833 MS.gene59840:CDS
AGTTTGAAGAACTTGTCAAC+GGG 0.511180 8.3:-54101846 None:intergenic
ATATTGACCCACCAAATGGC+TGG 0.520216 8.3:+54102943 MS.gene59840:CDS
GAGGATATTGACCCACCAAA+TGG 0.531703 8.3:+54102939 MS.gene59840:CDS
GGTAAACTTAGTACTGAACT+TGG 0.538495 8.3:+54101813 MS.gene59840:CDS
ATAACTTTCAAGCTCTTCGG+TGG 0.552870 8.3:+54102810 MS.gene59840:CDS
AAGTCCATCCAGCCATTTGG+TGG 0.553291 8.3:-54102951 None:intergenic
ATTGATAATGGTGATGGCAG+TGG 0.556824 8.3:+54102879 MS.gene59840:CDS
TGACCCACCAAATGGCTGGA+TGG 0.567137 8.3:+54102947 MS.gene59840:CDS
ATTGATCTTCTCATATTCAA+CGG 0.576809 8.3:-54102916 None:intergenic
GTTGATGCTCATCTTGTCAA+AGG 0.591021 8.3:+54102996 MS.gene59840:CDS
CATCATGCCAAACTTCATGA+AGG 0.593864 8.3:+54101915 MS.gene59840:CDS
TAGTTTGAAGAACTTGTCAA+CGG 0.601012 8.3:-54101847 None:intergenic
TGACATCACAATAGATGGCA+AGG 0.606404 8.3:+54102737 MS.gene59840:intron
GTAAACTTAGTACTGAACTT+GGG 0.614711 8.3:+54101814 MS.gene59840:CDS
GAAGTAATTGATAATGGTGA+TGG 0.618480 8.3:+54102873 MS.gene59840:CDS
ACACTGATTCAGTTAAACAC+TGG 0.620261 8.3:+54101952 MS.gene59840:CDS
AGTCCATCCAGCCATTTGGT+GGG 0.658787 8.3:-54102950 None:intergenic
AAACACTGGACTTATGTCGT+AGG 0.664039 8.3:+54101966 MS.gene59840:CDS
TGAATATGAGAAGATCAATG+AGG 0.699788 8.3:+54102920 MS.gene59840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATACATTGTTTTAATTTAAT+TGG + chr8.3:54102701-54102720 MS.gene59840:intron 10.0%
!! AGAATATATAGAATAAGTGA+TGG + chr8.3:54102328-54102347 MS.gene59840:intron 20.0%
!! ATCATTGAAGTAATTGATAA+TGG + chr8.3:54102867-54102886 MS.gene59840:CDS 20.0%
!! CATAATCTTCGTATAAAATA+TGG - chr8.3:54102116-54102135 None:intergenic 20.0%
!! GATTTACATGTCTAAATAAA+TGG + chr8.3:54102381-54102400 MS.gene59840:intron 20.0%
!! GTAGAGTTATGAAAAATATA+TGG - chr8.3:54102681-54102700 None:intergenic 20.0%
!! TAAAATGCTAAATACTCATT+AGG + chr8.3:54102449-54102468 MS.gene59840:intron 20.0%
!! TGCTCTTATTAATTGAATTA+AGG - chr8.3:54102087-54102106 None:intergenic 20.0%
!!! AATTCAATTAATAAGAGCAT+AGG + chr8.3:54102088-54102107 MS.gene59840:intron 20.0%
!!! ATTCAATTAATAAGAGCATA+GGG + chr8.3:54102089-54102108 MS.gene59840:intron 20.0%
! AAAATAACTTTCAAGCTCTT+CGG + chr8.3:54102807-54102826 MS.gene59840:CDS 25.0%
! AAATAAACAAGATGATGACT+TGG - chr8.3:54102024-54102043 None:intergenic 25.0%
! ACTTTATCTTTGTCATCTTT+AGG - chr8.3:54102140-54102159 None:intergenic 25.0%
! ATTGATCTTCTCATATTCAA+CGG - chr8.3:54102919-54102938 None:intergenic 25.0%
!! TATTCTCTCATTGATTGATT+TGG - chr8.3:54102314-54102333 None:intergenic 25.0%
AAAAATAAGACACTGTTTCC+TGG + chr8.3:54102186-54102205 MS.gene59840:intron 30.0%
ACAAATTCATATATGATGCC+AGG - chr8.3:54102207-54102226 None:intergenic 30.0%
ATAGATACGTAGTACATACT+TGG + chr8.3:54102412-54102431 MS.gene59840:intron 30.0%
CATATATGAATTTGTGCAGA+TGG + chr8.3:54102212-54102231 MS.gene59840:intron 30.0%
TAGTTTGAAGAACTTGTCAA+CGG - chr8.3:54101850-54101869 None:intergenic 30.0%
TGAATATGAGAAGATCAATG+AGG + chr8.3:54102920-54102939 MS.gene59840:CDS 30.0%
! GTAAACTTAGTACTGAACTT+GGG + chr8.3:54101814-54101833 MS.gene59840:CDS 30.0%
!! GAAGTAATTGATAATGGTGA+TGG + chr8.3:54102873-54102892 MS.gene59840:CDS 30.0%
AACTTCATGAAGGTGAAGAT+TGG + chr8.3:54101925-54101944 MS.gene59840:CDS 35.0%
AGACATTGATGAGCACTATA+AGG + chr8.3:54102833-54102852 MS.gene59840:CDS 35.0%
AGTTTGAAGAACTTGTCAAC+GGG - chr8.3:54101849-54101868 None:intergenic 35.0%
ATATGAATTTGTGCAGATGG+AGG + chr8.3:54102215-54102234 MS.gene59840:intron 35.0%
ATCTTCACCTTCATGAAGTT+TGG - chr8.3:54101925-54101944 None:intergenic 35.0%
! GGTAAACTTAGTACTGAACT+TGG + chr8.3:54101813-54101832 MS.gene59840:CDS 35.0%
!! AAGATGATGACTTGGTTATG+AGG - chr8.3:54102016-54102035 None:intergenic 35.0%
!! ACACTGATTCAGTTAAACAC+TGG + chr8.3:54101952-54101971 MS.gene59840:CDS 35.0%
AAACACTGGACTTATGTCGT+AGG + chr8.3:54101966-54101985 MS.gene59840:CDS 40.0%
AATAAGTCCATCCAGCCATT+TGG - chr8.3:54102957-54102976 None:intergenic 40.0%
ATAACTTTCAAGCTCTTCGG+TGG + chr8.3:54102810-54102829 MS.gene59840:CDS 40.0%
CATCATGCCAAACTTCATGA+AGG + chr8.3:54101915-54101934 MS.gene59840:CDS 40.0%
GCCATCTATTGTGATGTCAT+GGG - chr8.3:54102736-54102755 None:intergenic 40.0%
GTCCTAAACGATATGTGAGA+AGG + chr8.3:54102549-54102568 MS.gene59840:intron 40.0%
GTTGATGCTCATCTTGTCAA+AGG + chr8.3:54102996-54103015 MS.gene59840:CDS 40.0%
TCCCATGACATCACAATAGA+TGG + chr8.3:54102732-54102751 MS.gene59840:intron 40.0%
TGACATCACAATAGATGGCA+AGG + chr8.3:54102737-54102756 MS.gene59840:intron 40.0%
TGCCATCTATTGTGATGTCA+TGG - chr8.3:54102737-54102756 None:intergenic 40.0%
TGCCTTCTCACATATCGTTT+AGG - chr8.3:54102554-54102573 None:intergenic 40.0%
! AATGGCTGGATGGACTTATT+AGG + chr8.3:54102957-54102976 MS.gene59840:CDS 40.0%
!! ATTGATAATGGTGATGGCAG+TGG + chr8.3:54102879-54102898 MS.gene59840:CDS 40.0%
ATATTGACCCACCAAATGGC+TGG + chr8.3:54102943-54102962 MS.gene59840:CDS 45.0%
GAGGATATTGACCCACCAAA+TGG + chr8.3:54102939-54102958 MS.gene59840:CDS 45.0%
TTGTGCAGATGGAGGATCTA+TGG + chr8.3:54102223-54102242 MS.gene59840:intron 45.0%
AAGTCCATCCAGCCATTTGG+TGG - chr8.3:54102954-54102973 None:intergenic 50.0%
AGTCCATCCAGCCATTTGGT+GGG - chr8.3:54102953-54102972 None:intergenic 50.0%
ATGGCAGTGGAGCTGCAAAA+TGG + chr8.3:54102892-54102911 MS.gene59840:CDS 50.0%
CAGATGGAGGATCTATGGAG+AGG + chr8.3:54102228-54102247 MS.gene59840:intron 50.0%
TGACCCACCAAATGGCTGGA+TGG + chr8.3:54102947-54102966 MS.gene59840:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 54101801 54103031 54101801 ID=MS.gene59840
chr8.3 mRNA 54101801 54103031 54101801 ID=MS.gene59840.t1;Parent=MS.gene59840
chr8.3 exon 54101801 54101987 54101801 ID=MS.gene59840.t1.exon1;Parent=MS.gene59840.t1
chr8.3 CDS 54101801 54101987 54101801 ID=cds.MS.gene59840.t1;Parent=MS.gene59840.t1
chr8.3 exon 54102751 54103031 54102751 ID=MS.gene59840.t1.exon2;Parent=MS.gene59840.t1
chr8.3 CDS 54102751 54103031 54102751 ID=cds.MS.gene59840.t1;Parent=MS.gene59840.t1
Gene Sequence

>MS.gene59840

ATGGTGCTTGCTGGTAAACTTAGTACTGAACTTGGGATCAAAACACCCGTTGACAAGTTCTTCAAACTATATGCATCAGAACTTCACGAAGTGCAAAACATTAGTGAAAGAATTCATCATGCCAAACTTCATGAAGGTGAAGATTGGCATCACACTGATTCAGTTAAACACTGGACTTATGTCGTAGGTAATTAAGTTTCTAATTCAATTGACCTCATAACCAAGTCATCATCTTGTTTATTTATATATTTTTTTTGAACAATTTGTTTATTTATATATTGATCCTTAATTCAATTAATAAGAGCATAGGGACCATATTTTATACGAAGATTATGTCCTAAAGATGACAAAGATAAAGTTTTTCAGCTGTGAAATTTAGATACAAAAAAATAAGACACTGTTTCCTGGCATCATATATGAATTTGTGCAGATGGAGGATCTATGGAGAGGAAAAGATAAAAAAAATAAAACAGTCAGAAAAAAAAAACAATTCGATCAAAGTTTAATTAACCAAATCAATCAATGAGAGAATATATAGAATAAGTGATGGATTTATAGATACATCATAGTACATATACTTGATTTACATGTCTAAATAAATGGTAGTTACTATAGATACGTAGTACATACTTGGTATTCATGTCAAAATAAAATGCTAAATACTCATTAGGAGATACATGATTGACTTTATAGTTGTAATGAGTGCATATAATTCAGACTTCTTAAAGATATTCAAATATTGCATAGAGTCCTAAACGATATGTGAGAAGGCAACTAAACATTATATAGATTATTAATATTCATTTTTTTGTTTAAAAACTATTTTATTTGAAAAATGAAAACAATTTAATTGATTAGTTATTTTAAAAAAATGTGTCCATATATTTTTCATAACTCTACATACATTGTTTTAATTTAATTGGTATTAATTTCCCATGACATCACAATAGATGGCAAGGTAAACACATGTCATGAGAGTGTTGAAGAAGTTGATGAACAGAACAAAAAAATAACTTTCAAGCTCTTCGGTGGAGACATTGATGAGCACTATAAGGTCTTTAATCTGATCATTGAAGTAATTGATAATGGTGATGGCAGTGGAGCTGCAAAATGGACCGTTGAATATGAGAAGATCAATGAGGATATTGACCCACCAAATGGCTGGATGGACTTATTAGGCAAAATCACTCGAGAAGTTGATGCTCATCTTGTCAAAGGCACAAACATATAG

Protein sequence

>MS.gene59840.t1

MVLAGKLSTELGIKTPVDKFFKLYASELHEVQNISERIHHAKLHEGEDWHHTDSVKHWTYVVDGKVNTCHESVEEVDEQNKKITFKLFGGDIDEHYKVFNLIIEVIDNGDGSGAAKWTVEYEKINEDIDPPNGWMDLLGKITREVDAHLVKGTNI