AlfalfaGEDB | MS.gene77781

M. sativa cultivar XinJiangDaYe / MS.gene77781

KEGG: -
GO: GO:0006950 GO:0006952 GO:0008150 GO:0050896
PFAM: PF00407.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77781.t1	XP_003628192.1	92.3	155	11	1	1	155	1	154	4.20E-79	303.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77781.t1	Q9SSK9	36.7	150	92	3	3	150	22	170	2.0e-21	103.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77781.t1	G7L7S7	92.3	155	11	1	1	155	1	154	3.1e-79	303.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene77781	0.816479	5.42E-52	-1.69E-46
MS.gene049254	MS.gene77781	0.81126	7.65E-51	-1.69E-46
MS.gene049272	MS.gene77781	0.810838	9.45E-51	-1.69E-46
MS.gene049404	MS.gene77781	0.810028	1.41E-50	-1.69E-46
MS.gene049435	MS.gene77781	0.830081	3.59E-55	-1.69E-46
MS.gene049501	MS.gene77781	0.833326	5.69E-56	-1.69E-46
MS.gene049850	MS.gene77781	0.833389	5.49E-56	-1.69E-46
MS.gene050020	MS.gene77781	0.814595	1.42E-51	-1.69E-46
MS.gene050021	MS.gene77781	0.826917	2.09E-54	-1.69E-46
MS.gene050026	MS.gene77781	0.807425	5.09E-50	-1.69E-46
MS.gene050137	MS.gene77781	0.854275	1.37E-61	-1.69E-46
MS.gene050156	MS.gene77781	0.836435	9.38E-57	-1.69E-46
MS.gene05016	MS.gene77781	0.824309	8.66E-54	-1.69E-46
MS.gene050201	MS.gene77781	0.805758	1.14E-49	-1.69E-46
MS.gene050226	MS.gene77781	0.815265	1.01E-51	-1.69E-46
MS.gene050227	MS.gene77781	0.821088	4.86E-53	-1.69E-46
MS.gene050228	MS.gene77781	0.815861	7.44E-52	-1.69E-46
MS.gene050355	MS.gene77781	0.816324	5.86E-52	-1.69E-46
MS.gene050442	MS.gene77781	0.839744	1.32E-57	-1.69E-46
MS.gene050680	MS.gene77781	0.84016	1.03E-57	-1.69E-46
MS.gene050750	MS.gene77781	0.814122	1.81E-51	-1.69E-46
MS.gene050780	MS.gene77781	0.810541	1.10E-50	-1.69E-46
MS.gene050787	MS.gene77781	0.848976	4.35E-60	-1.69E-46
MS.gene050807	MS.gene77781	0.832253	1.05E-55	-1.69E-46
MS.gene050820	MS.gene77781	0.807343	5.29E-50	-1.69E-46
MS.gene050826	MS.gene77781	0.818852	1.58E-52	-1.69E-46
MS.gene050844	MS.gene77781	0.835764	1.39E-56	-1.69E-46
MS.gene050953	MS.gene77781	0.804426	2.17E-49	-1.69E-46
MS.gene050959	MS.gene77781	0.802524	5.39E-49	-1.69E-46
MS.gene051190	MS.gene77781	0.805479	1.31E-49	-1.69E-46
MS.gene051247	MS.gene77781	0.823497	1.34E-53	-1.69E-46
MS.gene05125	MS.gene77781	0.800178	1.63E-48	-1.69E-46
MS.gene051265	MS.gene77781	0.861156	1.25E-63	-1.69E-46
MS.gene051279	MS.gene77781	0.801239	9.89E-49	-1.69E-46
MS.gene05132	MS.gene77781	0.808049	3.75E-50	-1.69E-46
MS.gene051594	MS.gene77781	0.81301	3.18E-51	-1.69E-46
MS.gene051719	MS.gene77781	0.835816	1.35E-56	-1.69E-46
MS.gene051720	MS.gene77781	0.826706	2.34E-54	-1.69E-46
MS.gene051744	MS.gene77781	0.803053	4.19E-49	-1.69E-46
MS.gene051800	MS.gene77781	0.812584	3.94E-51	-1.69E-46
MS.gene051957	MS.gene77781	0.805202	1.50E-49	-1.69E-46
MS.gene052020	MS.gene77781	0.823211	1.57E-53	-1.69E-46
MS.gene052042	MS.gene77781	0.817186	3.76E-52	-1.69E-46
MS.gene052190	MS.gene77781	0.851435	8.89E-61	-1.69E-46
MS.gene052323	MS.gene77781	0.808165	3.54E-50	-1.69E-46
MS.gene052333	MS.gene77781	0.842971	1.87E-58	-1.69E-46
MS.gene052488	MS.gene77781	0.823922	1.07E-53	-1.69E-46
MS.gene052516	MS.gene77781	0.804442	2.16E-49	-1.69E-46
MS.gene052519	MS.gene77781	0.827401	1.60E-54	-1.69E-46
MS.gene052582	MS.gene77781	0.814074	1.85E-51	-1.69E-46
MS.gene052584	MS.gene77781	0.81606	6.72E-52	-1.69E-46
MS.gene052611	MS.gene77781	0.837743	4.34E-57	-1.69E-46
MS.gene052612	MS.gene77781	0.803487	3.41E-49	-1.69E-46
MS.gene052613	MS.gene77781	0.818247	2.16E-52	-1.69E-46
MS.gene052773	MS.gene77781	0.814855	1.25E-51	-1.69E-46
MS.gene052829	MS.gene77781	0.80235	5.85E-49	-1.69E-46
MS.gene052863	MS.gene77781	0.834177	3.49E-56	-1.69E-46
MS.gene052867	MS.gene77781	0.818287	2.12E-52	-1.69E-46
MS.gene052896	MS.gene77781	0.819668	1.03E-52	-1.69E-46
MS.gene053008	MS.gene77781	0.809074	2.27E-50	-1.69E-46
MS.gene053054	MS.gene77781	-0.812766	3.59E-51	-1.69E-46
MS.gene053147	MS.gene77781	0.822262	2.60E-53	-1.69E-46
MS.gene053166	MS.gene77781	0.865635	5.12E-65	-1.69E-46
MS.gene053201	MS.gene77781	0.810455	1.14E-50	-1.69E-46
MS.gene053221	MS.gene77781	0.831759	1.39E-55	-1.69E-46
MS.gene053226	MS.gene77781	0.829256	5.70E-55	-1.69E-46
MS.gene053331	MS.gene77781	0.827285	1.70E-54	-1.69E-46
MS.gene053584	MS.gene77781	0.810204	1.30E-50	-1.69E-46
MS.gene053966	MS.gene77781	0.813999	1.93E-51	-1.69E-46
MS.gene053974	MS.gene77781	0.810626	1.05E-50	-1.69E-46
MS.gene053998	MS.gene77781	0.809903	1.50E-50	-1.69E-46
MS.gene05414	MS.gene77781	0.822696	2.06E-53	-1.69E-46
MS.gene05415	MS.gene77781	0.842877	1.98E-58	-1.69E-46
MS.gene054236	MS.gene77781	0.826993	2.00E-54	-1.69E-46
MS.gene054291	MS.gene77781	-0.847271	1.29E-59	-1.69E-46
MS.gene054433	MS.gene77781	0.80359	3.24E-49	-1.69E-46
MS.gene054467	MS.gene77781	0.879357	1.33E-69	-1.69E-46
MS.gene054468	MS.gene77781	0.867	1.89E-65	-1.69E-46
MS.gene054472	MS.gene77781	0.815949	7.11E-52	-1.69E-46
MS.gene054559	MS.gene77781	0.842387	2.67E-58	-1.69E-46
MS.gene054581	MS.gene77781	0.818111	2.32E-52	-1.69E-46
MS.gene054610	MS.gene77781	0.809926	1.49E-50	-1.69E-46
MS.gene054651	MS.gene77781	0.805254	1.46E-49	-1.69E-46
MS.gene05468	MS.gene77781	0.827311	1.68E-54	-1.69E-46
MS.gene054775	MS.gene77781	0.813914	2.01E-51	-1.69E-46
MS.gene054801	MS.gene77781	0.803955	2.72E-49	-1.69E-46
MS.gene055166	MS.gene77781	0.808318	3.29E-50	-1.69E-46
MS.gene055227	MS.gene77781	0.846046	2.78E-59	-1.69E-46
MS.gene055228	MS.gene77781	0.813276	2.78E-51	-1.69E-46
MS.gene055356	MS.gene77781	0.880563	4.95E-70	-1.69E-46
MS.gene055357	MS.gene77781	0.868457	6.44E-66	-1.69E-46
MS.gene055358	MS.gene77781	0.890076	1.37E-73	-1.69E-46
MS.gene055465	MS.gene77781	0.80807	3.71E-50	-1.69E-46
MS.gene055466	MS.gene77781	0.824418	8.16E-54	-1.69E-46
MS.gene055507	MS.gene77781	0.819732	9.95E-53	-1.69E-46
MS.gene055701	MS.gene77781	0.807573	4.73E-50	-1.69E-46
MS.gene055804	MS.gene77781	0.820189	7.82E-53	-1.69E-46
MS.gene055818	MS.gene77781	0.858712	6.82E-63	-1.69E-46
MS.gene055926	MS.gene77781	0.821693	3.52E-53	-1.69E-46
MS.gene05604	MS.gene77781	0.863701	2.06E-64	-1.69E-46
MS.gene056050	MS.gene77781	0.805336	1.40E-49	-1.69E-46
MS.gene056173	MS.gene77781	0.829046	6.41E-55	-1.69E-46
MS.gene056174	MS.gene77781	0.804553	2.04E-49	-1.69E-46
MS.gene056366	MS.gene77781	0.810756	9.84E-51	-1.69E-46
MS.gene056506	MS.gene77781	0.823482	1.35E-53	-1.69E-46
MS.gene056542	MS.gene77781	0.809254	2.07E-50	-1.69E-46
MS.gene056543	MS.gene77781	0.808199	3.48E-50	-1.69E-46
MS.gene056545	MS.gene77781	0.81228	4.59E-51	-1.69E-46
MS.gene056553	MS.gene77781	0.83267	8.28E-56	-1.69E-46
MS.gene056595	MS.gene77781	0.814754	1.31E-51	-1.69E-46
MS.gene056643	MS.gene77781	0.804889	1.74E-49	-1.69E-46
MS.gene057021	MS.gene77781	0.803686	3.10E-49	-1.69E-46
MS.gene057026	MS.gene77781	0.852634	4.06E-61	-1.69E-46
MS.gene057027	MS.gene77781	0.812097	5.03E-51	-1.69E-46
MS.gene057060	MS.gene77781	0.84123	5.39E-58	-1.69E-46
MS.gene057314	MS.gene77781	0.816075	6.66E-52	-1.69E-46
MS.gene057414	MS.gene77781	0.808544	2.94E-50	-1.69E-46
MS.gene057444	MS.gene77781	0.835106	2.04E-56	-1.69E-46
MS.gene057693	MS.gene77781	0.816763	4.68E-52	-1.69E-46
MS.gene057869	MS.gene77781	0.807647	4.56E-50	-1.69E-46
MS.gene057915	MS.gene77781	0.836357	9.81E-57	-1.69E-46
MS.gene058010	MS.gene77781	0.804226	2.39E-49	-1.69E-46
MS.gene058037	MS.gene77781	0.801382	9.25E-49	-1.69E-46
MS.gene058168	MS.gene77781	0.801721	7.88E-49	-1.69E-46
MS.gene058294	MS.gene77781	0.802861	4.59E-49	-1.69E-46
MS.gene058625	MS.gene77781	0.84773	9.61E-60	-1.69E-46
MS.gene058829	MS.gene77781	0.844014	9.82E-59	-1.69E-46
MS.gene058979	MS.gene77781	0.829302	5.55E-55	-1.69E-46
MS.gene059015	MS.gene77781	0.801595	8.36E-49	-1.69E-46
MS.gene059128	MS.gene77781	0.841567	4.39E-58	-1.69E-46
MS.gene059161	MS.gene77781	0.819883	9.19E-53	-1.69E-46
MS.gene059272	MS.gene77781	0.831607	1.52E-55	-1.69E-46
MS.gene059382	MS.gene77781	0.809174	2.16E-50	-1.69E-46
MS.gene059584	MS.gene77781	0.825202	5.33E-54	-1.69E-46
MS.gene059607	MS.gene77781	0.849507	3.09E-60	-1.69E-46
MS.gene059789	MS.gene77781	0.804849	1.77E-49	-1.69E-46
MS.gene060011	MS.gene77781	0.80456	2.04E-49	-1.69E-46
MS.gene060137	MS.gene77781	-0.824858	6.43E-54	-1.69E-46
MS.gene060167	MS.gene77781	0.814063	1.86E-51	-1.69E-46
MS.gene060312	MS.gene77781	0.830719	2.51E-55	-1.69E-46
MS.gene060322	MS.gene77781	0.874533	6.27E-68	-1.69E-46
MS.gene06052	MS.gene77781	0.812663	3.79E-51	-1.69E-46
MS.gene060553	MS.gene77781	0.812871	3.41E-51	-1.69E-46
MS.gene060572	MS.gene77781	0.814807	1.28E-51	-1.69E-46
MS.gene060685	MS.gene77781	0.803249	3.82E-49	-1.69E-46
MS.gene060690	MS.gene77781	0.800694	1.28E-48	-1.69E-46
MS.gene060781	MS.gene77781	0.862803	3.91E-64	-1.69E-46
MS.gene060803	MS.gene77781	0.814153	1.78E-51	-1.69E-46
MS.gene060998	MS.gene77781	0.808737	2.67E-50	-1.69E-46
MS.gene061040	MS.gene77781	-0.804664	1.94E-49	-1.69E-46
MS.gene061114	MS.gene77781	-0.825061	5.75E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene62988	MS.gene77781	PPI
MS.gene59140	MS.gene77781	PPI
MS.gene74353	MS.gene77781	PPI
MS.gene77771	MS.gene77781	PPI
MS.gene59840	MS.gene77781	PPI
MS.gene59869	MS.gene77781	PPI
MS.gene74354	MS.gene77781	PPI
MS.gene016314	MS.gene77781	PPI
MS.gene016330	MS.gene77781	PPI
MS.gene77758	MS.gene77781	PPI
MS.gene59860	MS.gene77781	PPI
MS.gene59859	MS.gene77781	PPI
MS.gene026605	MS.gene77781	PPI
MS.gene058383	MS.gene77781	PPI
MS.gene74341	MS.gene77781	PPI
MS.gene058382	MS.gene77781	PPI
MS.gene77781	MS.gene59859	PPI
MS.gene77780	MS.gene77781	PPI
MS.gene74365	MS.gene77781	PPI
MS.gene59847	MS.gene77781	PPI
MS.gene77768	MS.gene77781	PPI
MS.gene74346	MS.gene77781	PPI
MS.gene59863	MS.gene77781	PPI
MS.gene59841	MS.gene77781	PPI
MS.gene77781	MS.gene59840	PPI
MS.gene77770	MS.gene77781	PPI
MS.gene74342	MS.gene77781	PPI
MS.gene016307	MS.gene77781	PPI
MS.gene59849	MS.gene77781	PPI
MS.gene058384	MS.gene77781	PPI
MS.gene74355	MS.gene77781	PPI
MS.gene058386	MS.gene77781	PPI
MS.gene016317	MS.gene77781	PPI
MS.gene74343	MS.gene77781	PPI
MS.gene77774	MS.gene77781	PPI
MS.gene77761	MS.gene77781	PPI
MS.gene77763	MS.gene77781	PPI
MS.gene77769	MS.gene77781	PPI
MS.gene59857	MS.gene77781	PPI
MS.gene74364	MS.gene77781	PPI
MS.gene016327	MS.gene77781	PPI
MS.gene74352	MS.gene77781	PPI
MS.gene74367	MS.gene77781	PPI
MS.gene59862	MS.gene77781	PPI
MS.gene016336	MS.gene77781	PPI
MS.gene77781	MS.gene016317	PPI
MS.gene74358	MS.gene77781	PPI
MS.gene74344	MS.gene77781	PPI
MS.gene99548	MS.gene77781	PPI
MS.gene066940	MS.gene77781	PPI
MS.gene016315	MS.gene77781	PPI
MS.gene016326	MS.gene77781	PPI
MS.gene016337	MS.gene77781	PPI
MS.gene83129	MS.gene77781	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77781.t1	MTR_8g045400	92.258	155	11	1	1	155	1	154	4.06e-103	292
MS.gene77781.t1	MTR_8g045490	78.289	152	33	0	1	152	1	152	2.66e-89	257
MS.gene77781.t1	MTR_8g045520	79.355	155	32	0	1	155	1	155	2.81e-89	257
MS.gene77781.t1	MTR_8g045695	74.839	155	38	1	1	155	1	154	3.54e-84	244
MS.gene77781.t1	MTR_8g045735	71.613	155	43	1	1	155	1	154	1.95e-80	234
MS.gene77781.t1	MTR_8g045570	71.053	152	44	0	1	152	1	152	2.56e-80	234
MS.gene77781.t1	MTR_8g045640	74.194	155	39	1	1	155	1	154	5.53e-80	233
MS.gene77781.t1	MTR_8g045665	72.903	155	41	1	1	155	1	154	5.91e-80	233
MS.gene77781.t1	MTR_8g045560	72.903	155	41	1	1	155	1	154	1.83e-79	232
MS.gene77781.t1	MTR_8g045555	70.395	152	45	0	1	152	1	152	1.37e-78	230
MS.gene77781.t1	MTR_8g045300	69.032	155	48	0	1	155	1	155	1.99e-78	229
MS.gene77781.t1	MTR_1g030820	37.255	153	93	2	1	151	1	152	1.68e-36	123
MS.gene77781.t1	MTR_1g030810	38.562	153	91	2	1	151	1	152	5.32e-36	122
MS.gene77781.t1	MTR_1g030840	37.580	157	88	5	1	149	1	155	7.78e-29	104
MS.gene77781.t1	MTR_8g012550	34.483	145	92	3	6	149	8	150	2.45e-27	100
MS.gene77781.t1	MTR_1g031640	37.162	148	84	5	7	152	7	147	8.76e-23	88.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77781.t1	AT5G28010	35.333	150	94	3	3	150	15	163	2.44e-27	100
MS.gene77781.t1	AT5G28010	35.333	150	94	3	3	150	15	163	2.44e-27	100
MS.gene77781.t1	AT1G70840	35.333	150	94	3	3	150	20	168	1.02e-25	96.7
MS.gene77781.t1	AT1G70830	36.667	150	92	3	3	150	22	170	2.45e-25	95.5
MS.gene77781.t1	AT1G70890	36.667	150	92	3	3	150	7	155	3.97e-25	94.7
MS.gene77781.t1	AT1G70830	36.667	150	92	3	3	150	22	170	4.90e-25	95.5
MS.gene77781.t1	AT1G70830	36.667	150	92	3	3	150	22	170	1.84e-24	95.1
MS.gene77781.t1	AT1G70830	36.667	150	92	3	3	150	22	170	5.92e-24	95.5
MS.gene77781.t1	AT1G70830	35.333	150	94	3	3	150	184	332	1.97e-22	91.3
MS.gene77781.t1	AT5G28000	34.000	150	96	3	3	150	13	161	1.06e-23	91.3
MS.gene77781.t1	AT1G70850	34.667	150	95	3	3	150	9	157	4.25e-23	91.3
MS.gene77781.t1	AT1G70830	35.333	150	94	3	3	150	137	285	6.76e-23	92.0
MS.gene77781.t1	AT1G70830	33.333	93	61	1	59	150	31	123	9.23e-13	64.3
MS.gene77781.t1	AT1G70850	34.667	150	95	3	3	150	9	157	1.38e-22	91.7
MS.gene77781.t1	AT1G70850	35.714	140	87	3	13	150	175	313	1.94e-21	88.6
MS.gene77781.t1	AT1G70850	34.667	150	95	3	3	150	9	157	1.38e-22	91.7
MS.gene77781.t1	AT1G70850	35.714	140	87	3	13	150	175	313	1.94e-21	88.6
MS.gene77781.t1	AT1G23130	29.530	149	103	2	3	150	10	157	2.81e-20	82.0
MS.gene77781.t1	AT1G70870	32.450	151	89	3	1	151	1	138	9.71e-20	80.1
MS.gene77781.t1	AT4G23670	32.680	153	100	3	1	152	1	151	2.27e-19	79.7
MS.gene77781.t1	AT1G70880	30.667	150	101	3	3	150	8	156	2.92e-19	79.7
MS.gene77781.t1	AT1G23120	27.333	150	104	3	1	150	1	145	5.98e-19	78.6
MS.gene77781.t1	AT1G23120	27.778	144	99	3	7	150	57	195	1.77e-18	78.6
MS.gene77781.t1	AT2G01520	29.412	153	105	3	1	152	1	151	2.67e-17	74.3
MS.gene77781.t1	AT1G14930	27.742	155	109	3	1	154	1	153	3.78e-17	73.9
MS.gene77781.t1	AT1G35260	28.767	146	100	3	7	150	6	149	1.80e-16	72.0
MS.gene77781.t1	AT2G01530	30.065	153	104	3	1	152	1	151	2.28e-16	72.0
MS.gene77781.t1	AT4G14060	29.412	153	105	3	1	152	1	151	2.94e-16	71.6
MS.gene77781.t1	AT4G23680	28.289	152	106	3	1	151	1	150	1.35e-15	69.7
MS.gene77781.t1	AT1G14940	27.097	155	110	3	1	154	1	153	2.05e-14	67.0
MS.gene77781.t1	AT1G30990	29.801	151	102	4	1	150	1	148	2.12e-14	66.6
MS.gene77781.t1	AT1G14950	27.742	155	109	3	1	154	1	153	3.63e-14	66.2
MS.gene77781.t1	AT3G26450	29.221	154	105	4	1	152	1	152	5.27e-14	65.9
MS.gene77781.t1	AT1G24020	29.677	155	107	2	1	154	1	154	1.99e-13	64.3
MS.gene77781.t1	AT1G24020	29.677	155	107	2	1	154	1	154	1.99e-13	64.3
MS.gene77781.t1	AT1G35310	25.180	139	101	3	13	150	12	148	6.10e-13	62.8
MS.gene77781.t1	AT1G14960	27.632	152	107	3	1	151	1	150	1.07e-11	59.7
MS.gene77781.t1	AT3G26460	29.487	156	102	5	1	152	1	152	1.61e-11	59.3

Find 28 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTTCACCTTCATGAAGTT+TGG	0.292428	8.1:-60116066	None:intergenic
CATTCAGAAATGGTGCTTGC+TGG	0.350620	8.1:+60115936	None:intergenic
AATGGCTGGATGGACTTATT+AGG	0.352875	8.1:+60117071	MS.gene77781:CDS
AAAATAACTTTCAAGCTCTT+CGG	0.428417	8.1:+60116921	MS.gene77781:CDS
ATCATTGAAGTAATTGATAA+TGG	0.436398	8.1:+60116981	MS.gene77781:CDS
ATGGCAGTGGAGCTGCAAAA+TGG	0.452179	8.1:+60117006	MS.gene77781:CDS
AATAAGTCCATCCAGCCATT+TGG	0.456616	8.1:-60117068	None:intergenic
AAACACTGGACTTATGTCAT+AGG	0.490184	8.1:+60116110	MS.gene77781:CDS
AACTTCATGAAGGTGAAGAT+TGG	0.494334	8.1:+60116069	MS.gene77781:CDS
AGACATTGATGAGCACTATA+AGG	0.495099	8.1:+60116947	MS.gene77781:CDS
AGTTTGAAGAACTTGTCAAC+GGG	0.511180	8.1:-60115990	None:intergenic
ATATTGACCCACCAAATGGC+TGG	0.520216	8.1:+60117057	MS.gene77781:CDS
GAGGATATTGACCCACCAAA+TGG	0.531703	8.1:+60117053	MS.gene77781:CDS
GGTAAACTTAGTACTGAACT+TGG	0.538495	8.1:+60115957	MS.gene77781:CDS
CTTCATGCCAAACTTCATGA+AGG	0.543027	8.1:+60116059	MS.gene77781:CDS
ATAACTTTCAAGCTCTTCGG+TGG	0.552870	8.1:+60116924	MS.gene77781:CDS
AAGTCCATCCAGCCATTTGG+TGG	0.553291	8.1:-60117065	None:intergenic
ATTGATAATGGTGATGGCAG+TGG	0.556824	8.1:+60116993	MS.gene77781:CDS
TGACCCACCAAATGGCTGGA+TGG	0.567137	8.1:+60117061	MS.gene77781:CDS
ATTGATCTTCTCATATTCAA+CGG	0.576809	8.1:-60117030	None:intergenic
GTTGATGCTCATCTTGTCAA+AGG	0.591021	8.1:+60117110	MS.gene77781:CDS
TAGTTTGAAGAACTTGTCAA+CGG	0.601012	8.1:-60115991	None:intergenic
TGACATCACAATAGATGGCA+AGG	0.606404	8.1:+60116851	MS.gene77781:intron
GTAAACTTAGTACTGAACTT+GGG	0.614711	8.1:+60115958	MS.gene77781:CDS
GAAGTAATTGATAATGGTGA+TGG	0.618480	8.1:+60116987	MS.gene77781:CDS
ACACTGATTCAGTTAAACAC+TGG	0.620261	8.1:+60116096	MS.gene77781:CDS
AGTCCATCCAGCCATTTGGT+GGG	0.658787	8.1:-60117064	None:intergenic
TGAATATGAGAAGATCAATG+AGG	0.699788	8.1:+60117034	MS.gene77781:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GTACATTGTTTTAATTTAAT+TGG	+	chr8.1:60116815-60116834	MS.gene77781:intron	15.0%
!!	ACAATATATAGAATAAGTGA+TGG	+	chr8.1:60116442-60116461	MS.gene77781:intron	20.0%
!!	ATCATTGAAGTAATTGATAA+TGG	+	chr8.1:60116981-60117000	MS.gene77781:CDS	20.0%
!!	CATAATCTTCGTATAAAATA+TGG	-	chr8.1:60116230-60116249	None:intergenic	20.0%
!!	GATTTACATGTCTAAATAAA+TGG	+	chr8.1:60116495-60116514	MS.gene77781:intron	20.0%
!!	GTAGAGTTATGAAAAATATA+TGG	-	chr8.1:60116795-60116814	None:intergenic	20.0%
!!	TAAAATGCTAAATACTCATT+AGG	+	chr8.1:60116563-60116582	MS.gene77781:intron	20.0%
!!	TGCTCTTATTAATTGAATTA+AGG	-	chr8.1:60116201-60116220	None:intergenic	20.0%
!!!	AATTCAATTAATAAGAGCAT+AGG	+	chr8.1:60116202-60116221	MS.gene77781:intron	20.0%
!!!	ATTCAATTAATAAGAGCATA+GGG	+	chr8.1:60116203-60116222	MS.gene77781:intron	20.0%
!	AAAATAACTTTCAAGCTCTT+CGG	+	chr8.1:60116921-60116940	MS.gene77781:CDS	25.0%
!	AAATAAACAAGATGATGACT+TGG	-	chr8.1:60116168-60116187	None:intergenic	25.0%
!	ACTTTATCTTTGTCATCTTT+AGG	-	chr8.1:60116254-60116273	None:intergenic	25.0%
!	ATTGATCTTCTCATATTCAA+CGG	-	chr8.1:60117033-60117052	None:intergenic	25.0%
!!	TATTGTCTCATTGATTGATT+TGG	-	chr8.1:60116428-60116447	None:intergenic	25.0%
	AAAAATAAGACACTGTTTCC+TGG	+	chr8.1:60116300-60116319	MS.gene77781:intron	30.0%
	AAAGATACGTAGTACATACT+TGG	+	chr8.1:60116526-60116545	MS.gene77781:intron	30.0%
	ACAAATTCATATATGATGCC+AGG	-	chr8.1:60116321-60116340	None:intergenic	30.0%
	CATATATGAATTTGTGCAGA+TGG	+	chr8.1:60116326-60116345	MS.gene77781:intron	30.0%
	TAGTTTGAAGAACTTGTCAA+CGG	-	chr8.1:60115994-60116013	None:intergenic	30.0%
	TGAATATGAGAAGATCAATG+AGG	+	chr8.1:60117034-60117053	MS.gene77781:CDS	30.0%
!	GTAAACTTAGTACTGAACTT+GGG	+	chr8.1:60115958-60115977	MS.gene77781:CDS	30.0%
!!	GAAGTAATTGATAATGGTGA+TGG	+	chr8.1:60116987-60117006	MS.gene77781:CDS	30.0%
	AAACACTGGACTTATGTCAT+AGG	+	chr8.1:60116110-60116129	MS.gene77781:CDS	35.0%
	AACTTCATGAAGGTGAAGAT+TGG	+	chr8.1:60116069-60116088	MS.gene77781:CDS	35.0%
	AGACATTGATGAGCACTATA+AGG	+	chr8.1:60116947-60116966	MS.gene77781:CDS	35.0%
	AGTTTGAAGAACTTGTCAAC+GGG	-	chr8.1:60115993-60116012	None:intergenic	35.0%
	ATATGAATTTGTGCAGATGG+AGG	+	chr8.1:60116329-60116348	MS.gene77781:intron	35.0%
	ATCTTCACCTTCATGAAGTT+TGG	-	chr8.1:60116069-60116088	None:intergenic	35.0%
!	GGTAAACTTAGTACTGAACT+TGG	+	chr8.1:60115957-60115976	MS.gene77781:CDS	35.0%
!!	AAGATGATGACTTGGTTATG+AGG	-	chr8.1:60116160-60116179	None:intergenic	35.0%
!!	ACACTGATTCAGTTAAACAC+TGG	+	chr8.1:60116096-60116115	MS.gene77781:CDS	35.0%
	AATAAGTCCATCCAGCCATT+TGG	-	chr8.1:60117071-60117090	None:intergenic	40.0%
	ATAACTTTCAAGCTCTTCGG+TGG	+	chr8.1:60116924-60116943	MS.gene77781:CDS	40.0%
	CTTCATGCCAAACTTCATGA+AGG	+	chr8.1:60116059-60116078	MS.gene77781:CDS	40.0%
	GCCATCTATTGTGATGTCAT+GGG	-	chr8.1:60116850-60116869	None:intergenic	40.0%
	GTCCTAAACGATATGTGAGA+AGG	+	chr8.1:60116663-60116682	MS.gene77781:intron	40.0%
	GTTGATGCTCATCTTGTCAA+AGG	+	chr8.1:60117110-60117129	MS.gene77781:CDS	40.0%
	TCCCATGACATCACAATAGA+TGG	+	chr8.1:60116846-60116865	MS.gene77781:intron	40.0%
	TGACATCACAATAGATGGCA+AGG	+	chr8.1:60116851-60116870	MS.gene77781:intron	40.0%
	TGCCATCTATTGTGATGTCA+TGG	-	chr8.1:60116851-60116870	None:intergenic	40.0%
	TGCCTTCTCACATATCGTTT+AGG	-	chr8.1:60116668-60116687	None:intergenic	40.0%
!	AATGGCTGGATGGACTTATT+AGG	+	chr8.1:60117071-60117090	MS.gene77781:CDS	40.0%
!!	ATTGATAATGGTGATGGCAG+TGG	+	chr8.1:60116993-60117012	MS.gene77781:CDS	40.0%
	ATATTGACCCACCAAATGGC+TGG	+	chr8.1:60117057-60117076	MS.gene77781:CDS	45.0%
	GAGGATATTGACCCACCAAA+TGG	+	chr8.1:60117053-60117072	MS.gene77781:CDS	45.0%
	TTGTGCAGATGGAGGATCTA+TGG	+	chr8.1:60116337-60116356	MS.gene77781:intron	45.0%
	AAGTCCATCCAGCCATTTGG+TGG	-	chr8.1:60117068-60117087	None:intergenic	50.0%
	AGTCCATCCAGCCATTTGGT+GGG	-	chr8.1:60117067-60117086	None:intergenic	50.0%
	ATGGCAGTGGAGCTGCAAAA+TGG	+	chr8.1:60117006-60117025	MS.gene77781:CDS	50.0%
	CAGATGGAGGATCTATGGAG+AGG	+	chr8.1:60116342-60116361	MS.gene77781:intron	50.0%
	TGACCCACCAAATGGCTGGA+TGG	+	chr8.1:60117061-60117080	MS.gene77781:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	60115945	60117145	60115945	ID=MS.gene77781
chr8.1	mRNA	60115945	60117145	60115945	ID=MS.gene77781.t1;Parent=MS.gene77781
chr8.1	exon	60115945	60116131	60115945	ID=MS.gene77781.t1.exon1;Parent=MS.gene77781.t1
chr8.1	CDS	60115945	60116131	60115945	ID=cds.MS.gene77781.t1;Parent=MS.gene77781.t1
chr8.1	exon	60116865	60117145	60116865	ID=MS.gene77781.t1.exon2;Parent=MS.gene77781.t1
chr8.1	CDS	60116865	60117145	60116865	ID=cds.MS.gene77781.t1;Parent=MS.gene77781.t1

Gene Sequence

>MS.gene77781

ATGGTGCTTGCTGGTAAACTTAGTACTGAACTTGGGATCAAAACACCCGTTGACAAGTTCTTCAAACTATATGCATCAGAACTTCACGAAGTGCAAAACATTAGTGAAAGAATTCTTCATGCCAAACTTCATGAAGGTGAAGATTGGCATCACACTGATTCAGTTAAACACTGGACTTATGTCATAGGTAATTAAGTTTCTAATTCAATTGACCTCATAACCAAGTCATCATCTTGTTTATTTATATATTGATCCTTAATTCAATTAATAAGAGCATAGGGACCATATTTTATACGAAGATTATGTCCTAAAGATGACAAAGATAAAGTTTTTCAGCTGTGAAATTTAGATACAAAAAAATAAGACACTGTTTCCTGGCATCATATATGAATTTGTGCAGATGGAGGATCTATGGAGAGGAAAAGATAAAAAAAATAAAACAGTCAGAAAAAAAAAACAATTCGATCAAAGTTTAATTAACCAAATCAATCAATGAGACAATATATAGAATAAGTGATGGATTTATAGATACATCATAGTACATATACTTGATTTACATGTCTAAATAAATGGTAGTTACTAAAGATACGTAGTACATACTTGGTATTCATGTCAAAATAAAATGCTAAATACTCATTAGGAGATACATGATTGACTTTATAGTTGTAATGAGTGCATATAATTCAGACTTCTTAAAGATATTCAAATATTGCATAGAGTCCTAAACGATATGTGAGAAGGCAACTAAACATTATATAGATTATTAATATTCATTTTTTTGTTTAAAAACTATTTTATTTGAAAAATGAAAACAATTTAATTGATTAGTTATTTTAAAAAAATGTGTCCATATATTTTTCATAACTCTACGTACATTGTTTTAATTTAATTGGTATTAATTTCCCATGACATCACAATAGATGGCAAGGTAAACACATGTCATGAGAGTGTTGAAGAAGTTGATGAACAGAACAAAAAAATAACTTTCAAGCTCTTCGGTGGAGACATTGATGAGCACTATAAGGTCTTTAATCTGATCATTGAAGTAATTGATAATGGTGATGGCAGTGGAGCTGCAAAATGGACCGTTGAATATGAGAAGATCAATGAGGATATTGACCCACCAAATGGCTGGATGGACTTATTAGGCAAAATCACTCGAGAAGTTGATGCTCATCTTGTCAAAGGCACAAACATATAG

Protein sequence

>MS.gene77781.t1

MVLAGKLSTELGIKTPVDKFFKLYASELHEVQNISERILHAKLHEGEDWHHTDSVKHWTYVIDGKVNTCHESVEEVDEQNKKITFKLFGGDIDEHYKVFNLIIEVIDNGDGSGAAKWTVEYEKINEDIDPPNGWMDLLGKITREVDAHLVKGTNI