Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44240.t1 | XP_024629324.1 | 98.8 | 82 | 1 | 0 | 1 | 82 | 1 | 82 | 1.70E-38 | 169.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44240.t1 | P27484 | 65.4 | 81 | 25 | 1 | 1 | 78 | 1 | 81 | 6.5e-23 | 109.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44240.t1 | A0A396GLK6 | 98.8 | 82 | 1 | 0 | 1 | 82 | 1 | 82 | 1.2e-38 | 169.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene44240.t1 | TF | CSD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049129 | MS.gene44240 | -0.831091 | 2.03E-55 | -1.69E-46 |
| MS.gene049255 | MS.gene44240 | 0.83443 | 3.01E-56 | -1.69E-46 |
| MS.gene049283 | MS.gene44240 | 0.818585 | 1.82E-52 | -1.69E-46 |
| MS.gene049411 | MS.gene44240 | 0.842046 | 3.28E-58 | -1.69E-46 |
| MS.gene049580 | MS.gene44240 | 0.812774 | 3.58E-51 | -1.69E-46 |
| MS.gene050277 | MS.gene44240 | 0.822376 | 2.45E-53 | -1.69E-46 |
| MS.gene050646 | MS.gene44240 | 0.804663 | 1.94E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene44240 | 0.824369 | 8.38E-54 | -1.69E-46 |
| MS.gene050752 | MS.gene44240 | 0.864209 | 1.43E-64 | -1.69E-46 |
| MS.gene050838 | MS.gene44240 | 0.84955 | 3.01E-60 | -1.69E-46 |
| MS.gene050945 | MS.gene44240 | 0.861326 | 1.11E-63 | -1.69E-46 |
| MS.gene050955 | MS.gene44240 | 0.849939 | 2.34E-60 | -1.69E-46 |
| MS.gene051003 | MS.gene44240 | 0.856653 | 2.78E-62 | -1.69E-46 |
| MS.gene051043 | MS.gene44240 | 0.837582 | 4.77E-57 | -1.69E-46 |
| MS.gene051233 | MS.gene44240 | 0.856018 | 4.27E-62 | -1.69E-46 |
| MS.gene051234 | MS.gene44240 | 0.868079 | 8.52E-66 | -1.69E-46 |
| MS.gene051328 | MS.gene44240 | 0.820359 | 7.15E-53 | -1.69E-46 |
| MS.gene051343 | MS.gene44240 | 0.839515 | 1.51E-57 | -1.69E-46 |
| MS.gene05134 | MS.gene44240 | 0.829883 | 4.01E-55 | -1.69E-46 |
| MS.gene051699 | MS.gene44240 | 0.806411 | 8.34E-50 | -1.69E-46 |
| MS.gene05188 | MS.gene44240 | 0.82605 | 3.35E-54 | -1.69E-46 |
| MS.gene051902 | MS.gene44240 | 0.847775 | 9.34E-60 | -1.69E-46 |
| MS.gene051903 | MS.gene44240 | 0.822572 | 2.20E-53 | -1.69E-46 |
| MS.gene052072 | MS.gene44240 | 0.821738 | 3.44E-53 | -1.69E-46 |
| MS.gene052296 | MS.gene44240 | 0.808399 | 3.16E-50 | -1.69E-46 |
| MS.gene052342 | MS.gene44240 | 0.805255 | 1.46E-49 | -1.69E-46 |
| MS.gene052479 | MS.gene44240 | 0.833008 | 6.83E-56 | -1.69E-46 |
| MS.gene052947 | MS.gene44240 | 0.866263 | 3.24E-65 | -1.69E-46 |
| MS.gene053058 | MS.gene44240 | 0.878923 | 1.90E-69 | -1.69E-46 |
| MS.gene053122 | MS.gene44240 | 0.820738 | 5.85E-53 | -1.69E-46 |
| MS.gene053564 | MS.gene44240 | 0.819225 | 1.30E-52 | -1.69E-46 |
| MS.gene05364 | MS.gene44240 | 0.821467 | 3.98E-53 | -1.69E-46 |
| MS.gene053931 | MS.gene44240 | 0.818488 | 1.91E-52 | -1.69E-46 |
| MS.gene054004 | MS.gene44240 | 0.819549 | 1.10E-52 | -1.69E-46 |
| MS.gene054403 | MS.gene44240 | 0.8688 | 4.99E-66 | -1.69E-46 |
| MS.gene054413 | MS.gene44240 | 0.832321 | 1.01E-55 | -1.69E-46 |
| MS.gene05517 | MS.gene44240 | 0.803679 | 3.11E-49 | -1.69E-46 |
| MS.gene055424 | MS.gene44240 | 0.823496 | 1.34E-53 | -1.69E-46 |
| MS.gene05548 | MS.gene44240 | 0.813526 | 2.45E-51 | -1.69E-46 |
| MS.gene055555 | MS.gene44240 | 0.824213 | 9.12E-54 | -1.69E-46 |
| MS.gene055765 | MS.gene44240 | 0.816032 | 6.81E-52 | -1.69E-46 |
| MS.gene055808 | MS.gene44240 | 0.804271 | 2.34E-49 | -1.69E-46 |
| MS.gene055979 | MS.gene44240 | 0.811776 | 5.91E-51 | -1.69E-46 |
| MS.gene056075 | MS.gene44240 | 0.803099 | 4.10E-49 | -1.69E-46 |
| MS.gene056215 | MS.gene44240 | 0.817769 | 2.78E-52 | -1.69E-46 |
| MS.gene05648 | MS.gene44240 | 0.805008 | 1.64E-49 | -1.69E-46 |
| MS.gene056580 | MS.gene44240 | 0.804369 | 2.23E-49 | -1.69E-46 |
| MS.gene05689 | MS.gene44240 | 0.801165 | 1.02E-48 | -1.69E-46 |
| MS.gene057094 | MS.gene44240 | 0.801201 | 1.01E-48 | -1.69E-46 |
| MS.gene057102 | MS.gene44240 | 0.810081 | 1.38E-50 | -1.69E-46 |
| MS.gene057234 | MS.gene44240 | 0.810358 | 1.20E-50 | -1.69E-46 |
| MS.gene057576 | MS.gene44240 | 0.807549 | 4.79E-50 | -1.69E-46 |
| MS.gene057694 | MS.gene44240 | 0.832944 | 7.08E-56 | -1.69E-46 |
| MS.gene057705 | MS.gene44240 | 0.899676 | 1.56E-77 | -1.69E-46 |
| MS.gene05781 | MS.gene44240 | 0.864752 | 9.70E-65 | -1.69E-46 |
| MS.gene057890 | MS.gene44240 | -0.80201 | 6.87E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene44240 | 0.814288 | 1.66E-51 | -1.69E-46 |
| MS.gene058054 | MS.gene44240 | 0.820022 | 8.54E-53 | -1.69E-46 |
| MS.gene058055 | MS.gene44240 | 0.811859 | 5.67E-51 | -1.69E-46 |
| MS.gene058074 | MS.gene44240 | 0.837539 | 4.90E-57 | -1.69E-46 |
| MS.gene058126 | MS.gene44240 | 0.800526 | 1.38E-48 | -1.69E-46 |
| MS.gene058238 | MS.gene44240 | 0.805738 | 1.15E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene44240 | 0.820361 | 7.15E-53 | -1.69E-46 |
| MS.gene059146 | MS.gene44240 | 0.867768 | 1.07E-65 | -1.69E-46 |
| MS.gene05978 | MS.gene44240 | 0.815087 | 1.11E-51 | -1.69E-46 |
| MS.gene05985 | MS.gene44240 | -0.808558 | 2.92E-50 | -1.69E-46 |
| MS.gene059901 | MS.gene44240 | -0.809534 | 1.80E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene44240 | 0.813008 | 3.18E-51 | -1.69E-46 |
| MS.gene06011 | MS.gene44240 | 0.807901 | 4.03E-50 | -1.69E-46 |
| MS.gene060244 | MS.gene44240 | 0.802352 | 5.84E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene44240 | 0.812197 | 4.78E-51 | -1.69E-46 |
| MS.gene060429 | MS.gene44240 | 0.813651 | 2.30E-51 | -1.69E-46 |
| MS.gene060763 | MS.gene44240 | 0.853418 | 2.42E-61 | -1.69E-46 |
| MS.gene060917 | MS.gene44240 | 0.805327 | 1.41E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene44240 | 0.827119 | 1.87E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene28445 | MS.gene44240 | PPI |
| MS.gene030675 | MS.gene44240 | PPI |
| MS.gene70271 | MS.gene44240 | PPI |
| MS.gene58125 | MS.gene44240 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44240.t1 | AT4G36020 | 45.196 | 281 | 126 | 7 | 1 | 274 | 6 | 265 | 3.16e-56 | 182 |
| MS.gene44240.t1 | AT4G36020 | 43.648 | 307 | 127 | 8 | 1 | 274 | 6 | 299 | 5.57e-56 | 182 |
| MS.gene44240.t1 | AT4G36020 | 43.648 | 307 | 127 | 8 | 1 | 274 | 6 | 299 | 5.57e-56 | 182 |
| MS.gene44240.t1 | AT2G17870 | 45.667 | 300 | 125 | 7 | 5 | 274 | 10 | 301 | 4.37e-46 | 157 |
| MS.gene44240.t1 | AT3G42860 | 31.387 | 137 | 72 | 3 | 160 | 274 | 223 | 359 | 3.25e-15 | 75.1 |
| MS.gene44240.t1 | AT3G42860 | 35.000 | 140 | 69 | 4 | 94 | 219 | 228 | 359 | 2.33e-14 | 72.8 |
| MS.gene44240.t1 | AT3G43590 | 23.611 | 216 | 90 | 5 | 101 | 275 | 164 | 345 | 3.74e-11 | 63.5 |
Find 106 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAACGGTGGCGGTGGATA+TGG | 0.297469 | 8.1:+31629439 | MS.gene44240:CDS |
| AGGGATTGTGTTGAGGCTTC+TGG | 0.315257 | 8.1:+31629751 | MS.gene44240:CDS |
| TCCTAAGGGTGAACCTCTTC+AGG | 0.319731 | 8.1:+31629162 | MS.gene44240:CDS |
| GGTGGAGGCGGCGGTGGTCG+TGG | 0.346511 | 8.1:+31629202 | MS.gene44240:CDS |
| AACTATGGTGGAGGCGGCGG+TGG | 0.381171 | 8.1:+31629196 | MS.gene44240:CDS |
| CCGCCGCCGCCTCCGCTACT+AGG | 0.384433 | 8.1:-31629523 | None:intergenic |
| ACCGGTGACATCAACGGCTT+TGG | 0.389735 | 8.1:-31629140 | None:intergenic |
| AGAAGGTTTGGCGGTGGTGG+TGG | 0.393791 | 8.1:+31629670 | MS.gene44240:CDS |
| TGTTATACTTGTGGTGATAC+TGG | 0.397825 | 8.1:+31629262 | MS.gene44240:CDS |
| GTGTTGAGGCTTCTGGTTGA+TGG | 0.398672 | 8.1:+31629758 | MS.gene44240:CDS |
| AACAGAAGGTTTGGCGGTGG+TGG | 0.399361 | 8.1:+31629667 | MS.gene44240:CDS |
| TTTGATGAATCCGAAGCCTT+TGG | 0.409445 | 8.1:-31628996 | None:intergenic |
| AGTAGCGGAGGCGGCGGCGG+TGG | 0.413340 | 8.1:+31629526 | MS.gene44240:CDS |
| GGTGGTGGCAACAGAAGGTT+TGG | 0.419148 | 8.1:+31629658 | MS.gene44240:CDS |
| GGCGGTGGTCGTGGATTTAG+AGG | 0.421752 | 8.1:+31629211 | MS.gene44240:CDS |
| TGCTACAGGTGTGGTGGGGT+TGG | 0.430495 | 8.1:+31629475 | MS.gene44240:CDS |
| CGTTGATGTCACCGGTCCTA+AGG | 0.435362 | 8.1:+31629147 | MS.gene44240:CDS |
| TTCAACAACACCAAAGGCTT+CGG | 0.440293 | 8.1:+31628986 | MS.gene44240:CDS |
| AGGTTTGGCGGTGGTGGTGG+CGG | 0.444094 | 8.1:+31629673 | MS.gene44240:CDS |
| GATAACTATGGTGGAGGCGG+CGG | 0.448297 | 8.1:+31629193 | MS.gene44240:CDS |
| CATCTTGCTACAGGTGTGGT+GGG | 0.457817 | 8.1:+31629470 | MS.gene44240:CDS |
| TGCTTTAACTGTGGGAAGGC+AGG | 0.462177 | 8.1:+31629718 | MS.gene44240:CDS |
| GGCGGTCGCGATGGAGGAAA+AGG | 0.463269 | 8.1:+31629691 | MS.gene44240:CDS |
| GATCGCTCTGGTGGTGGTGG+TGG | 0.464907 | 8.1:+31629331 | MS.gene44240:CDS |
| GAGATCTTCGCTATCGTCAT+CGG | 0.473073 | 8.1:-31629020 | None:intergenic |
| CCTAGTAGCGGAGGCGGCGG+CGG | 0.475047 | 8.1:+31629523 | MS.gene44240:CDS |
| GATGGCAATGGAAGGTTTGG+TGG | 0.475514 | 8.1:+31629640 | MS.gene44240:CDS |
| AGATCTTCGCTATCGTCATC+GGG | 0.477231 | 8.1:-31629019 | None:intergenic |
| AACAACAACAACGGTGGCGG+TGG | 0.478614 | 8.1:+31629433 | MS.gene44240:CDS |
| GTGAGGTGGGTCACATAGCT+AGG | 0.482831 | 8.1:+31629566 | MS.gene44240:CDS |
| GATGATGGCAATGGAAGGTT+TGG | 0.485258 | 8.1:+31629637 | MS.gene44240:CDS |
| GGAAGGTATGATGGTGGCAA+TGG | 0.489704 | 8.1:+31629607 | MS.gene44240:CDS |
| TTGCTATAAGTGCGGTGAGG+TGG | 0.492734 | 8.1:+31629552 | MS.gene44240:CDS |
| ACCTGAAGAGGTTCACCCTT+AGG | 0.495108 | 8.1:-31629163 | None:intergenic |
| GCTAGGGATTGCAGCAATGA+AGG | 0.497533 | 8.1:+31629583 | MS.gene44240:CDS |
| GTGATACTGGTCACATCGCT+AGG | 0.498797 | 8.1:+31629275 | MS.gene44240:CDS |
| ACATCTTGCTACAGGTGTGG+TGG | 0.503337 | 8.1:+31629469 | MS.gene44240:CDS |
| GGTGGTGGCGGTCGCGATGG+AGG | 0.503410 | 8.1:+31629685 | MS.gene44240:CDS |
| GAGAGAAGGAACGGTGGTGG+TGG | 0.512448 | 8.1:+31629238 | MS.gene44240:CDS |
| AGGGTGAACCTCTTCAGGTT+AGG | 0.518271 | 8.1:+31629167 | MS.gene44240:CDS |
| GTGGAGGCACATCTTGCTAC+AGG | 0.518304 | 8.1:+31629461 | MS.gene44240:CDS |
| TGCTATAAGTGCGGTGAGGT+GGG | 0.518807 | 8.1:+31629553 | MS.gene44240:CDS |
| ATCGTTCCGATCGGAATGAT+AGG | 0.521685 | 8.1:+31629305 | MS.gene44240:CDS |
| GGTGGTGGTGGCGGTCGCGA+TGG | 0.528129 | 8.1:+31629682 | MS.gene44240:CDS |
| TGAACCTCTTCAGGTTAGGC+AGG | 0.528588 | 8.1:+31629171 | MS.gene44240:CDS |
| ACAATCGCCGACGGTGATAA+CGG | 0.532690 | 8.1:+31629112 | MS.gene44240:CDS |
| GGTGAGAGAAGGAACGGTGG+TGG | 0.532764 | 8.1:+31629235 | MS.gene44240:CDS |
| AATGATCGCTCTGGTGGTGG+TGG | 0.533030 | 8.1:+31629328 | MS.gene44240:CDS |
| GGTGGCAACAGAAGGTTTGG+CGG | 0.535347 | 8.1:+31629661 | MS.gene44240:CDS |
| TTTGCTAGGGATTGCATGCG+TGG | 0.536617 | 8.1:+31629403 | MS.gene44240:CDS |
| GGTATGATGGTGGCAATGGA+AGG | 0.540936 | 8.1:+31629611 | MS.gene44240:CDS |
| CAGGTTAGGCAGGATAACTA+TGG | 0.541696 | 8.1:+31629181 | MS.gene44240:CDS |
| CAATCTGACATCAGATCTGA+AGG | 0.544978 | 8.1:+31629052 | MS.gene44240:CDS |
| ACCAAAGCCGTTGATGTCAC+CGG | 0.545847 | 8.1:+31629139 | MS.gene44240:CDS |
| GAATCAGCGGCGTGGTGCAA+TGG | 0.549203 | 8.1:+31628963 | MS.gene44240:CDS |
| GACCGTGCTTGCTATACTTG+TGG | 0.551573 | 8.1:+31629367 | MS.gene44240:CDS |
| CAGGATAACTATGGTGGAGG+CGG | 0.552223 | 8.1:+31629190 | MS.gene44240:CDS |
| TTATCCTGCCTAACCTGAAG+AGG | 0.553752 | 8.1:-31629175 | None:intergenic |
| GGTTTGGTGGTGGCAACAGA+AGG | 0.556716 | 8.1:+31629653 | MS.gene44240:CDS |
| AACGGTGGCGGTGGATATGG+TGG | 0.558225 | 8.1:+31629442 | MS.gene44240:CDS |
| AATGAAGGAGGAAGGTATGA+TGG | 0.561449 | 8.1:+31629598 | MS.gene44240:CDS |
| AGAGGTGGTGAGAGAAGGAA+CGG | 0.569558 | 8.1:+31629229 | MS.gene44240:CDS |
| GATTTAGAGGTGGTGAGAGA+AGG | 0.570831 | 8.1:+31629224 | MS.gene44240:CDS |
| AAAGGCACATGCTTTAACTG+TGG | 0.571520 | 8.1:+31629709 | MS.gene44240:CDS |
| GGCAACAGAAGGTTTGGCGG+TGG | 0.573285 | 8.1:+31629664 | MS.gene44240:CDS |
| AGGAATGATCGCTCTGGTGG+TGG | 0.574103 | 8.1:+31629325 | MS.gene44240:CDS |
| AAGGCACATGCTTTAACTGT+GGG | 0.579793 | 8.1:+31629710 | MS.gene44240:CDS |
| AATGATAGGAATGATCGCTC+TGG | 0.580640 | 8.1:+31629319 | MS.gene44240:CDS |
| GTTGATGTCACCGGTCCTAA+GGG | 0.586474 | 8.1:+31629148 | MS.gene44240:CDS |
| AAGAGGTTCACCCTTAGGAC+CGG | 0.590248 | 8.1:-31629158 | None:intergenic |
| GGCAATGGAAGGTTTGGTGG+TGG | 0.602525 | 8.1:+31629643 | MS.gene44240:CDS |
| AACCACAAGTATAGCAAGCA+CGG | 0.605269 | 8.1:-31629369 | None:intergenic |
| ATCTTGCTACAGGTGTGGTG+GGG | 0.605984 | 8.1:+31629471 | MS.gene44240:CDS |
| CTTAGGACCGGTGACATCAA+CGG | 0.607581 | 8.1:-31629146 | None:intergenic |
| GGAAGGTATGATGATGGCAA+TGG | 0.610842 | 8.1:+31629628 | MS.gene44240:CDS |
| GGTGGCGGTGGATATGGTGG+AGG | 0.611131 | 8.1:+31629445 | MS.gene44240:CDS |
| GGTGGTGGTTGTTATACTTG+TGG | 0.612907 | 8.1:+31629253 | MS.gene44240:CDS |
| ATCATTCCTATCATTCCGAT+CGG | 0.613940 | 8.1:-31629311 | None:intergenic |
| AAGTATAGCAAGCACGGTCA+CGG | 0.614873 | 8.1:-31629363 | None:intergenic |
| TGAGGTGGGTCACATAGCTA+GGG | 0.619943 | 8.1:+31629567 | MS.gene44240:CDS |
| GTCGAGTTCACAATCGCCGA+CGG | 0.621401 | 8.1:+31629103 | MS.gene44240:CDS |
| GGTATGATGATGGCAATGGA+AGG | 0.623747 | 8.1:+31629632 | MS.gene44240:CDS |
| GGCAATGGAAGGTATGATGA+TGG | 0.625626 | 8.1:+31629622 | MS.gene44240:CDS |
| CACATGCTTTAACTGTGGGA+AGG | 0.629671 | 8.1:+31629714 | MS.gene44240:CDS |
| GTTAGGCAGGATAACTATGG+TGG | 0.630912 | 8.1:+31629184 | MS.gene44240:CDS |
| AGGCAGGATAACTATGGTGG+AGG | 0.633369 | 8.1:+31629187 | MS.gene44240:CDS |
| ACTCCTAGTAGCGGAGGCGG+CGG | 0.634571 | 8.1:+31629520 | MS.gene44240:CDS |
| AGGGATTGCAGCAATGAAGG+AGG | 0.636247 | 8.1:+31629586 | MS.gene44240:CDS |
| GGCACATCTTGCTACAGGTG+TGG | 0.637876 | 8.1:+31629466 | MS.gene44240:CDS |
| ATTGCAGCAATGAAGGAGGA+AGG | 0.639616 | 8.1:+31629590 | MS.gene44240:CDS |
| CAATGGTTCAACAACACCAA+AGG | 0.639643 | 8.1:+31628980 | MS.gene44240:CDS |
| TGATACTGGTCACATCGCTA+GGG | 0.645119 | 8.1:+31629276 | MS.gene44240:CDS |
| GGGATTGTGATCGTTCCGAT+CGG | 0.645526 | 8.1:+31629296 | MS.gene44240:CDS |
| TGCGCTACTCCTAGTAGCGG+AGG | 0.648929 | 8.1:+31629514 | MS.gene44240:CDS |
| GCTAGGGATTGCATGCGTGG+AGG | 0.656728 | 8.1:+31629406 | MS.gene44240:CDS |
| GGTGGTGAGAGAAGGAACGG+TGG | 0.661941 | 8.1:+31629232 | MS.gene44240:CDS |
| GCTACTCCTAGTAGCGGAGG+CGG | 0.666720 | 8.1:+31629517 | MS.gene44240:CDS |
| GATTGCGCTACTCCTAGTAG+CGG | 0.671173 | 8.1:+31629511 | MS.gene44240:CDS |
| GATAGGAATGATCGCTCTGG+TGG | 0.671256 | 8.1:+31629322 | MS.gene44240:CDS |
| GGTGGTCGTGGATTTAGAGG+TGG | 0.678731 | 8.1:+31629214 | MS.gene44240:CDS |
| GGAGGAGAGAATCAGCGGCG+TGG | 0.687679 | 8.1:+31628955 | MS.gene44240:CDS |
| GAAGGAGGAAGGTATGATGG+TGG | 0.690298 | 8.1:+31629601 | MS.gene44240:CDS |
| ATGGCGGAGGAGAGAATCAG+CGG | 0.694395 | 8.1:+31628950 | MS.gene44240:CDS |
| GGAGGTAACAACAACAACAA+CGG | 0.704702 | 8.1:+31629424 | MS.gene44240:CDS |
| GGTGGTGCTTGCTATAAGTG+CGG | 0.737528 | 8.1:+31629544 | MS.gene44240:CDS |
| TGCTTGCTATAAGTGCGGTG+AGG | 0.749667 | 8.1:+31629549 | MS.gene44240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCTTTTGAACATTTTGCTA+GGG | + | chr8.1:31629390-31629409 | MS.gene44240:CDS | 25.0% |
| ! | GTTCTTTTGAACATTTTGCT+AGG | + | chr8.1:31629389-31629408 | MS.gene44240:CDS | 30.0% |
| ATCATTCCTATCATTCCGAT+CGG | - | chr8.1:31629314-31629333 | None:intergenic | 35.0% | |
| TGTTATACTTGTGGTGATAC+TGG | + | chr8.1:31629262-31629281 | MS.gene44240:CDS | 35.0% | |
| AACCACAAGTATAGCAAGCA+CGG | - | chr8.1:31629372-31629391 | None:intergenic | 40.0% | |
| AAGGCACATGCTTTAACTGT+GGG | + | chr8.1:31629710-31629729 | MS.gene44240:CDS | 40.0% | |
| AATGATAGGAATGATCGCTC+TGG | + | chr8.1:31629319-31629338 | MS.gene44240:CDS | 40.0% | |
| CAATCTGACATCAGATCTGA+AGG | + | chr8.1:31629052-31629071 | MS.gene44240:CDS | 40.0% | |
| CAATGGTTCAACAACACCAA+AGG | + | chr8.1:31628980-31628999 | MS.gene44240:CDS | 40.0% | |
| GGAGGTAACAACAACAACAA+CGG | + | chr8.1:31629424-31629443 | MS.gene44240:CDS | 40.0% | |
| TTCAACAACACCAAAGGCTT+CGG | + | chr8.1:31628986-31629005 | MS.gene44240:CDS | 40.0% | |
| TTTGATGAATCCGAAGCCTT+TGG | - | chr8.1:31628999-31629018 | None:intergenic | 40.0% | |
| ! | AAAGGCACATGCTTTAACTG+TGG | + | chr8.1:31629709-31629728 | MS.gene44240:CDS | 40.0% |
| ! | AATGAAGGAGGAAGGTATGA+TGG | + | chr8.1:31629598-31629617 | MS.gene44240:CDS | 40.0% |
| ! | TTTTGCAAGGGATTGTGTTG+AGG | + | chr8.1:31629744-31629763 | MS.gene44240:CDS | 40.0% |
| AACAACAACAACAACGGTGG+CGG | + | chr8.1:31629430-31629449 | MS.gene44240:CDS | 45.0% | |
| AAGTATAGCAAGCACGGTCA+CGG | - | chr8.1:31629366-31629385 | None:intergenic | 45.0% | |
| AGATCTTCGCTATCGTCATC+GGG | - | chr8.1:31629022-31629041 | None:intergenic | 45.0% | |
| ATCGTTCCGATCGGAATGAT+AGG | + | chr8.1:31629305-31629324 | MS.gene44240:CDS | 45.0% | |
| ATTGCAGCAATGAAGGAGGA+AGG | + | chr8.1:31629590-31629609 | MS.gene44240:CDS | 45.0% | |
| CACATGCTTTAACTGTGGGA+AGG | + | chr8.1:31629714-31629733 | MS.gene44240:CDS | 45.0% | |
| CAGGTTAGGCAGGATAACTA+TGG | + | chr8.1:31629181-31629200 | MS.gene44240:CDS | 45.0% | |
| GAGATCTTCGCTATCGTCAT+CGG | - | chr8.1:31629023-31629042 | None:intergenic | 45.0% | |
| GATTTAGAGGTGGTGAGAGA+AGG | + | chr8.1:31629224-31629243 | MS.gene44240:CDS | 45.0% | |
| GGTAACAACAACAACAACGG+TGG | + | chr8.1:31629427-31629446 | MS.gene44240:CDS | 45.0% | |
| GTTAGGCAGGATAACTATGG+TGG | + | chr8.1:31629184-31629203 | MS.gene44240:CDS | 45.0% | |
| TGATACTGGTCACATCGCTA+GGG | + | chr8.1:31629276-31629295 | MS.gene44240:CDS | 45.0% | |
| TTATCCTGCCTAACCTGAAG+AGG | - | chr8.1:31629178-31629197 | None:intergenic | 45.0% | |
| ! | GAAGGCAGGACATTTTGCAA+GGG | + | chr8.1:31629732-31629751 | MS.gene44240:CDS | 45.0% |
| ! | GATGATGGCAATGGAAGGTT+TGG | + | chr8.1:31629637-31629656 | MS.gene44240:CDS | 45.0% |
| ! | GGATTTCGCTCTCTTTTCGA+AGG | + | chr8.1:31629073-31629092 | MS.gene44240:CDS | 45.0% |
| ! | GGCAATGGAAGGTATGATGA+TGG | + | chr8.1:31629622-31629641 | MS.gene44240:CDS | 45.0% |
| ! | GGTGGTGGTTGTTATACTTG+TGG | + | chr8.1:31629253-31629272 | MS.gene44240:CDS | 45.0% |
| !! | GGAAGGTATGATGATGGCAA+TGG | + | chr8.1:31629628-31629647 | MS.gene44240:CDS | 45.0% |
| !! | GGTATGATGATGGCAATGGA+AGG | + | chr8.1:31629632-31629651 | MS.gene44240:CDS | 45.0% |
| AAGAGGTTCACCCTTAGGAC+CGG | - | chr8.1:31629161-31629180 | None:intergenic | 50.0% | |
| ACAATCGCCGACGGTGATAA+CGG | + | chr8.1:31629112-31629131 | MS.gene44240:CDS | 50.0% | |
| ACATCTTGCTACAGGTGTGG+TGG | + | chr8.1:31629469-31629488 | MS.gene44240:CDS | 50.0% | |
| ACCAAAGCCGTTGATGTCAC+CGG | + | chr8.1:31629139-31629158 | MS.gene44240:CDS | 50.0% | |
| ACCTGAAGAGGTTCACCCTT+AGG | - | chr8.1:31629166-31629185 | None:intergenic | 50.0% | |
| AGAGGTGGTGAGAGAAGGAA+CGG | + | chr8.1:31629229-31629248 | MS.gene44240:CDS | 50.0% | |
| AGGCAGGATAACTATGGTGG+AGG | + | chr8.1:31629187-31629206 | MS.gene44240:CDS | 50.0% | |
| AGGGATTGCAGCAATGAAGG+AGG | + | chr8.1:31629586-31629605 | MS.gene44240:CDS | 50.0% | |
| AGGGATTGTGTTGAGGCTTC+TGG | + | chr8.1:31629751-31629770 | MS.gene44240:CDS | 50.0% | |
| ATCTTGCTACAGGTGTGGTG+GGG | + | chr8.1:31629471-31629490 | MS.gene44240:CDS | 50.0% | |
| CATCTTGCTACAGGTGTGGT+GGG | + | chr8.1:31629470-31629489 | MS.gene44240:CDS | 50.0% | |
| CTTAGGACCGGTGACATCAA+CGG | - | chr8.1:31629149-31629168 | None:intergenic | 50.0% | |
| GATAGGAATGATCGCTCTGG+TGG | + | chr8.1:31629322-31629341 | MS.gene44240:CDS | 50.0% | |
| GATGGCAATGGAAGGTTTGG+TGG | + | chr8.1:31629640-31629659 | MS.gene44240:CDS | 50.0% | |
| GATTGCGCTACTCCTAGTAG+CGG | + | chr8.1:31629511-31629530 | MS.gene44240:CDS | 50.0% | |
| GCTAGGGATTGCAGCAATGA+AGG | + | chr8.1:31629583-31629602 | MS.gene44240:CDS | 50.0% | |
| GGGATTGTGATCGTTCCGAT+CGG | + | chr8.1:31629296-31629315 | MS.gene44240:CDS | 50.0% | |
| GTGATACTGGTCACATCGCT+AGG | + | chr8.1:31629275-31629294 | MS.gene44240:CDS | 50.0% | |
| GTTGATGTCACCGGTCCTAA+GGG | + | chr8.1:31629148-31629167 | MS.gene44240:CDS | 50.0% | |
| TCCTAAGGGTGAACCTCTTC+AGG | + | chr8.1:31629162-31629181 | MS.gene44240:CDS | 50.0% | |
| TGAGGTGGGTCACATAGCTA+GGG | + | chr8.1:31629567-31629586 | MS.gene44240:CDS | 50.0% | |
| TGCTATAAGTGCGGTGAGGT+GGG | + | chr8.1:31629553-31629572 | MS.gene44240:CDS | 50.0% | |
| TGCTTTAACTGTGGGAAGGC+AGG | + | chr8.1:31629718-31629737 | MS.gene44240:CDS | 50.0% | |
| TTGCTATAAGTGCGGTGAGG+TGG | + | chr8.1:31629552-31629571 | MS.gene44240:CDS | 50.0% | |
| TTTGCTAGGGATTGCATGCG+TGG | + | chr8.1:31629403-31629422 | MS.gene44240:CDS | 50.0% | |
| ! | AGGGTGAACCTCTTCAGGTT+AGG | + | chr8.1:31629167-31629186 | MS.gene44240:CDS | 50.0% |
| ! | CAGGATAACTATGGTGGAGG+CGG | + | chr8.1:31629190-31629209 | MS.gene44240:CDS | 50.0% |
| ! | GAAGGAGGAAGGTATGATGG+TGG | + | chr8.1:31629601-31629620 | MS.gene44240:CDS | 50.0% |
| ! | GGAAGGCAGGACATTTTGCA+AGG | + | chr8.1:31629731-31629750 | MS.gene44240:CDS | 50.0% |
| ! | GGTTTTGCCGTTATCACCGT+CGG | - | chr8.1:31629122-31629141 | None:intergenic | 50.0% |
| ! | TGAACCTCTTCAGGTTAGGC+AGG | + | chr8.1:31629171-31629190 | MS.gene44240:CDS | 50.0% |
| !! | GACCGTGCTTGCTATACTTG+TGG | + | chr8.1:31629367-31629386 | MS.gene44240:CDS | 50.0% |
| !! | GGAAGGTATGATGGTGGCAA+TGG | + | chr8.1:31629607-31629626 | MS.gene44240:CDS | 50.0% |
| !! | GGTATGATGGTGGCAATGGA+AGG | + | chr8.1:31629611-31629630 | MS.gene44240:CDS | 50.0% |
| !! | GGTGGTGCTTGCTATAAGTG+CGG | + | chr8.1:31629544-31629563 | MS.gene44240:CDS | 50.0% |
| !! | TGCTTGCTATAAGTGCGGTG+AGG | + | chr8.1:31629549-31629568 | MS.gene44240:CDS | 50.0% |
| AACAGAAGGTTTGGCGGTGG+TGG | + | chr8.1:31629667-31629686 | MS.gene44240:CDS | 55.0% | |
| ACCGGTGACATCAACGGCTT+TGG | - | chr8.1:31629143-31629162 | None:intergenic | 55.0% | |
| AGGAATGATCGCTCTGGTGG+TGG | + | chr8.1:31629325-31629344 | MS.gene44240:CDS | 55.0% | |
| CGTTGATGTCACCGGTCCTA+AGG | + | chr8.1:31629147-31629166 | MS.gene44240:CDS | 55.0% | |
| GGCACATCTTGCTACAGGTG+TGG | + | chr8.1:31629466-31629485 | MS.gene44240:CDS | 55.0% | |
| GGTGGCAACAGAAGGTTTGG+CGG | + | chr8.1:31629661-31629680 | MS.gene44240:CDS | 55.0% | |
| GGTGGTCGTGGATTTAGAGG+TGG | + | chr8.1:31629214-31629233 | MS.gene44240:CDS | 55.0% | |
| GTCGAGTTCACAATCGCCGA+CGG | + | chr8.1:31629103-31629122 | MS.gene44240:CDS | 55.0% | |
| GTGAGGTGGGTCACATAGCT+AGG | + | chr8.1:31629566-31629585 | MS.gene44240:CDS | 55.0% | |
| ! | AACAACAACAACGGTGGCGG+TGG | + | chr8.1:31629433-31629452 | MS.gene44240:CDS | 55.0% |
| ! | AACAACGGTGGCGGTGGATA+TGG | + | chr8.1:31629439-31629458 | MS.gene44240:CDS | 55.0% |
| ! | ATGGCGGAGGAGAGAATCAG+CGG | + | chr8.1:31628950-31628969 | MS.gene44240:CDS | 55.0% |
| ! | GATAACTATGGTGGAGGCGG+CGG | + | chr8.1:31629193-31629212 | MS.gene44240:CDS | 55.0% |
| ! | GGTGGTGGCAACAGAAGGTT+TGG | + | chr8.1:31629658-31629677 | MS.gene44240:CDS | 55.0% |
| ! | GTGGAGGCACATCTTGCTAC+AGG | + | chr8.1:31629461-31629480 | MS.gene44240:CDS | 55.0% |
| !! | AATGATCGCTCTGGTGGTGG+TGG | + | chr8.1:31629328-31629347 | MS.gene44240:CDS | 55.0% |
| !! | GGCAATGGAAGGTTTGGTGG+TGG | + | chr8.1:31629643-31629662 | MS.gene44240:CDS | 55.0% |
| !! | GGTTTGGTGGTGGCAACAGA+AGG | + | chr8.1:31629653-31629672 | MS.gene44240:CDS | 55.0% |
| GAATCAGCGGCGTGGTGCAA+TGG | + | chr8.1:31628963-31628982 | MS.gene44240:CDS | 60.0% | |
| GCTAGGGATTGCATGCGTGG+AGG | + | chr8.1:31629406-31629425 | MS.gene44240:CDS | 60.0% | |
| GGCAACAGAAGGTTTGGCGG+TGG | + | chr8.1:31629664-31629683 | MS.gene44240:CDS | 60.0% | |
| GGCGGTGGTCGTGGATTTAG+AGG | + | chr8.1:31629211-31629230 | MS.gene44240:CDS | 60.0% | |
| GGTGAGAGAAGGAACGGTGG+TGG | + | chr8.1:31629235-31629254 | MS.gene44240:CDS | 60.0% | |
| GGTGGTGAGAGAAGGAACGG+TGG | + | chr8.1:31629232-31629251 | MS.gene44240:CDS | 60.0% | |
| TGCGCTACTCCTAGTAGCGG+AGG | + | chr8.1:31629514-31629533 | MS.gene44240:CDS | 60.0% | |
| ! | AACGGTGGCGGTGGATATGG+TGG | + | chr8.1:31629442-31629461 | MS.gene44240:CDS | 60.0% |
| ! | AGAAGGTTTGGCGGTGGTGG+TGG | + | chr8.1:31629670-31629689 | MS.gene44240:CDS | 60.0% |
| ! | GAGAGAAGGAACGGTGGTGG+TGG | + | chr8.1:31629238-31629257 | MS.gene44240:CDS | 60.0% |
| ! | GCTACTCCTAGTAGCGGAGG+CGG | + | chr8.1:31629517-31629536 | MS.gene44240:CDS | 60.0% |
| ! | TGCTACAGGTGTGGTGGGGT+TGG | + | chr8.1:31629475-31629494 | MS.gene44240:CDS | 60.0% |
| GGAGGAGAGAATCAGCGGCG+TGG | + | chr8.1:31628955-31628974 | MS.gene44240:CDS | 65.0% | |
| GGCGGTCGCGATGGAGGAAA+AGG | + | chr8.1:31629691-31629710 | MS.gene44240:CDS | 65.0% | |
| ! | AACTATGGTGGAGGCGGCGG+TGG | + | chr8.1:31629196-31629215 | MS.gene44240:CDS | 65.0% |
| ! | ACTCCTAGTAGCGGAGGCGG+CGG | + | chr8.1:31629520-31629539 | MS.gene44240:CDS | 65.0% |
| ! | GGTGGCGGTGGATATGGTGG+AGG | + | chr8.1:31629445-31629464 | MS.gene44240:CDS | 65.0% |
| !! | AGGTTTGGCGGTGGTGGTGG+CGG | + | chr8.1:31629673-31629692 | MS.gene44240:CDS | 65.0% |
| !! | GATCGCTCTGGTGGTGGTGG+TGG | + | chr8.1:31629331-31629350 | MS.gene44240:CDS | 65.0% |
| !! | TCTGGTGGTGGTGGTGGTGG+TGG | + | chr8.1:31629337-31629356 | MS.gene44240:CDS | 65.0% |
| !! | CGCTCTGGTGGTGGTGGTGG+TGG | + | chr8.1:31629334-31629353 | MS.gene44240:CDS | 70.0% |
| ! | CCTAGTAGCGGAGGCGGCGG+CGG | + | chr8.1:31629523-31629542 | MS.gene44240:CDS | 75.0% |
| !! | GGTGGTGGCGGTCGCGATGG+AGG | + | chr8.1:31629685-31629704 | MS.gene44240:CDS | 75.0% |
| !! | GGTGGTGGTGGCGGTCGCGA+TGG | + | chr8.1:31629682-31629701 | MS.gene44240:CDS | 75.0% |
| ! | CCGCCGCCGCCTCCGCTACT+AGG | - | chr8.1:31629526-31629545 | None:intergenic | 80.0% |
| ! | GGTGGAGGCGGCGGTGGTCG+TGG | + | chr8.1:31629202-31629221 | MS.gene44240:CDS | 80.0% |
| !! | AGTAGCGGAGGCGGCGGCGG+TGG | + | chr8.1:31629526-31629545 | MS.gene44240:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 31628950 | 31629777 | 31628950 | ID=MS.gene44240 |
| chr8.1 | mRNA | 31628950 | 31629777 | 31628950 | ID=MS.gene44240.t1;Parent=MS.gene44240 |
| chr8.1 | exon | 31628950 | 31629777 | 31628950 | ID=MS.gene44240.t1.exon1;Parent=MS.gene44240.t1 |
| chr8.1 | CDS | 31628950 | 31629777 | 31628950 | ID=cds.MS.gene44240.t1;Parent=MS.gene44240.t1 |
| Gene Sequence |
| Protein sequence |
