Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62423.t1 | XP_003609315.1 | 100 | 345 | 0 | 0 | 1 | 345 | 1 | 345 | 2.40E-183 | 651.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62423.t1 | Q3E6T0 | 81.7 | 344 | 63 | 0 | 2 | 345 | 6 | 349 | 3.3e-157 | 555.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62423.t1 | G7JCY0 | 100.0 | 345 | 0 | 0 | 1 | 345 | 1 | 345 | 1.7e-183 | 651.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene62423 | 0.814757 | 1.31E-51 | -1.69E-46 |
| MS.gene049283 | MS.gene62423 | 0.805057 | 1.60E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene62423 | 0.836867 | 7.28E-57 | -1.69E-46 |
| MS.gene049711 | MS.gene62423 | 0.830478 | 2.87E-55 | -1.69E-46 |
| MS.gene049807 | MS.gene62423 | 0.880281 | 6.25E-70 | -1.69E-46 |
| MS.gene049887 | MS.gene62423 | 0.849965 | 2.30E-60 | -1.69E-46 |
| MS.gene050359 | MS.gene62423 | 0.804276 | 2.33E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene62423 | 0.837087 | 6.39E-57 | -1.69E-46 |
| MS.gene050559 | MS.gene62423 | 0.803571 | 3.27E-49 | -1.69E-46 |
| MS.gene050646 | MS.gene62423 | 0.831575 | 1.54E-55 | -1.69E-46 |
| MS.gene050752 | MS.gene62423 | 0.805233 | 1.47E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene62423 | 0.84268 | 2.23E-58 | -1.69E-46 |
| MS.gene050945 | MS.gene62423 | 0.837927 | 3.89E-57 | -1.69E-46 |
| MS.gene051003 | MS.gene62423 | 0.828577 | 8.32E-55 | -1.69E-46 |
| MS.gene051234 | MS.gene62423 | 0.874707 | 5.47E-68 | -1.69E-46 |
| MS.gene051343 | MS.gene62423 | 0.828201 | 1.03E-54 | -1.69E-46 |
| MS.gene051628 | MS.gene62423 | 0.819054 | 1.42E-52 | -1.69E-46 |
| MS.gene051699 | MS.gene62423 | 0.832244 | 1.06E-55 | -1.69E-46 |
| MS.gene051852 | MS.gene62423 | 0.826847 | 2.17E-54 | -1.69E-46 |
| MS.gene051885 | MS.gene62423 | 0.818872 | 1.56E-52 | -1.69E-46 |
| MS.gene051902 | MS.gene62423 | 0.801586 | 8.39E-49 | -1.69E-46 |
| MS.gene052016 | MS.gene62423 | 0.80374 | 3.02E-49 | -1.69E-46 |
| MS.gene052072 | MS.gene62423 | 0.823879 | 1.09E-53 | -1.69E-46 |
| MS.gene052239 | MS.gene62423 | -0.823992 | 1.03E-53 | -1.69E-46 |
| MS.gene052242 | MS.gene62423 | -0.834904 | 2.29E-56 | -1.69E-46 |
| MS.gene052307 | MS.gene62423 | 0.869321 | 3.38E-66 | -1.69E-46 |
| MS.gene052335 | MS.gene62423 | 0.843884 | 1.06E-58 | -1.69E-46 |
| MS.gene052463 | MS.gene62423 | 0.841017 | 6.13E-58 | -1.69E-46 |
| MS.gene052514 | MS.gene62423 | 0.800112 | 1.68E-48 | -1.69E-46 |
| MS.gene052516 | MS.gene62423 | 0.837872 | 4.02E-57 | -1.69E-46 |
| MS.gene052533 | MS.gene62423 | 0.851691 | 7.52E-61 | -1.69E-46 |
| MS.gene052609 | MS.gene62423 | 0.846193 | 2.53E-59 | -1.69E-46 |
| MS.gene053079 | MS.gene62423 | 0.837242 | 5.84E-57 | -1.69E-46 |
| MS.gene053122 | MS.gene62423 | 0.809803 | 1.58E-50 | -1.69E-46 |
| MS.gene053143 | MS.gene62423 | 0.878387 | 2.93E-69 | -1.69E-46 |
| MS.gene053144 | MS.gene62423 | 0.853248 | 2.71E-61 | -1.69E-46 |
| MS.gene053171 | MS.gene62423 | 0.814934 | 1.20E-51 | -1.69E-46 |
| MS.gene053581 | MS.gene62423 | 0.8356 | 1.53E-56 | -1.69E-46 |
| MS.gene053599 | MS.gene62423 | 0.807072 | 6.04E-50 | -1.69E-46 |
| MS.gene05364 | MS.gene62423 | 0.810186 | 1.31E-50 | -1.69E-46 |
| MS.gene053678 | MS.gene62423 | 0.800232 | 1.59E-48 | -1.69E-46 |
| MS.gene054004 | MS.gene62423 | 0.801277 | 9.72E-49 | -1.69E-46 |
| MS.gene054178 | MS.gene62423 | 0.831598 | 1.52E-55 | -1.69E-46 |
| MS.gene054403 | MS.gene62423 | 0.807149 | 5.82E-50 | -1.69E-46 |
| MS.gene054554 | MS.gene62423 | 0.827782 | 1.29E-54 | -1.69E-46 |
| MS.gene054584 | MS.gene62423 | 0.845271 | 4.51E-59 | -1.69E-46 |
| MS.gene054651 | MS.gene62423 | 0.802071 | 6.68E-49 | -1.69E-46 |
| MS.gene054709 | MS.gene62423 | 0.835991 | 1.22E-56 | -1.69E-46 |
| MS.gene054824 | MS.gene62423 | 0.828612 | 8.16E-55 | -1.69E-46 |
| MS.gene055386 | MS.gene62423 | 0.805254 | 1.46E-49 | -1.69E-46 |
| MS.gene055469 | MS.gene62423 | 0.814784 | 1.29E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene62423 | 0.836711 | 7.97E-57 | -1.69E-46 |
| MS.gene055580 | MS.gene62423 | -0.80466 | 1.94E-49 | -1.69E-46 |
| MS.gene055891 | MS.gene62423 | 0.802486 | 5.48E-49 | -1.69E-46 |
| MS.gene056043 | MS.gene62423 | -0.805554 | 1.26E-49 | -1.69E-46 |
| MS.gene056308 | MS.gene62423 | 0.83279 | 7.73E-56 | -1.69E-46 |
| MS.gene056390 | MS.gene62423 | 0.814584 | 1.43E-51 | -1.69E-46 |
| MS.gene056507 | MS.gene62423 | 0.812636 | 3.84E-51 | -1.69E-46 |
| MS.gene056578 | MS.gene62423 | 0.83535 | 1.77E-56 | -1.69E-46 |
| MS.gene056677 | MS.gene62423 | 0.858154 | 1.00E-62 | -1.69E-46 |
| MS.gene056699 | MS.gene62423 | 0.829881 | 4.02E-55 | -1.69E-46 |
| MS.gene057102 | MS.gene62423 | 0.870119 | 1.86E-66 | -1.69E-46 |
| MS.gene057576 | MS.gene62423 | 0.807037 | 6.15E-50 | -1.69E-46 |
| MS.gene058008 | MS.gene62423 | 0.807359 | 5.25E-50 | -1.69E-46 |
| MS.gene058149 | MS.gene62423 | 0.820274 | 7.48E-53 | -1.69E-46 |
| MS.gene058261 | MS.gene62423 | 0.803754 | 3.00E-49 | -1.69E-46 |
| MS.gene058330 | MS.gene62423 | 0.823937 | 1.06E-53 | -1.69E-46 |
| MS.gene058492 | MS.gene62423 | 0.853848 | 1.82E-61 | -1.69E-46 |
| MS.gene058649 | MS.gene62423 | 0.802249 | 6.14E-49 | -1.69E-46 |
| MS.gene058668 | MS.gene62423 | 0.819653 | 1.04E-52 | -1.69E-46 |
| MS.gene058852 | MS.gene62423 | 0.821568 | 3.77E-53 | -1.69E-46 |
| MS.gene059146 | MS.gene62423 | 0.817551 | 3.11E-52 | -1.69E-46 |
| MS.gene059170 | MS.gene62423 | 0.824594 | 7.42E-54 | -1.69E-46 |
| MS.gene059171 | MS.gene62423 | 0.803955 | 2.72E-49 | -1.69E-46 |
| MS.gene059227 | MS.gene62423 | 0.807816 | 4.20E-50 | -1.69E-46 |
| MS.gene059778 | MS.gene62423 | 0.818691 | 1.72E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene62423 | -0.812757 | 3.61E-51 | -1.69E-46 |
| MS.gene059965 | MS.gene62423 | 0.845036 | 5.22E-59 | -1.69E-46 |
| MS.gene060603 | MS.gene62423 | 0.806187 | 9.29E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene62423 | 0.800416 | 1.46E-48 | -1.69E-46 |
| MS.gene060935 | MS.gene62423 | 0.800391 | 1.47E-48 | -1.69E-46 |
| MS.gene060937 | MS.gene62423 | 0.815445 | 9.21E-52 | -1.69E-46 |
| MS.gene061006 | MS.gene62423 | 0.820503 | 6.63E-53 | -1.69E-46 |
| MS.gene061100 | MS.gene62423 | 0.875367 | 3.26E-68 | -1.69E-46 |
| MS.gene061160 | MS.gene62423 | 0.838194 | 3.32E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62423.t1 | MTR_4g114360 | 100.000 | 345 | 0 | 0 | 1 | 345 | 1 | 345 | 0.0 | 700 |
| MS.gene62423.t1 | MTR_3g107470 | 84.302 | 344 | 54 | 0 | 2 | 345 | 6 | 349 | 0.0 | 610 |
| MS.gene62423.t1 | MTR_0011s0320 | 83.381 | 349 | 53 | 3 | 1 | 344 | 1 | 349 | 0.0 | 588 |
| MS.gene62423.t1 | MTR_0662s0010 | 100.000 | 277 | 0 | 0 | 1 | 277 | 1 | 277 | 0.0 | 556 |
| MS.gene62423.t1 | MTR_7g110380 | 44.940 | 336 | 181 | 3 | 9 | 343 | 10 | 342 | 3.20e-104 | 311 |
| MS.gene62423.t1 | MTR_4g123900 | 50.000 | 306 | 151 | 2 | 9 | 313 | 6 | 310 | 2.61e-98 | 296 |
| MS.gene62423.t1 | MTR_4g123900 | 50.871 | 287 | 139 | 2 | 9 | 294 | 6 | 291 | 5.26e-94 | 282 |
| MS.gene62423.t1 | MTR_2g070400 | 43.302 | 321 | 179 | 3 | 13 | 331 | 10 | 329 | 1.92e-85 | 261 |
| MS.gene62423.t1 | MTR_3g070000 | 32.441 | 299 | 182 | 6 | 18 | 305 | 29 | 318 | 4.46e-33 | 125 |
| MS.gene62423.t1 | MTR_8g090130 | 34.007 | 297 | 180 | 9 | 18 | 305 | 16 | 305 | 1.63e-32 | 123 |
| MS.gene62423.t1 | MTR_5g081710 | 32.558 | 301 | 183 | 6 | 16 | 305 | 11 | 302 | 3.65e-32 | 122 |
| MS.gene62423.t1 | MTR_3g462070 | 30.693 | 303 | 194 | 6 | 12 | 305 | 8 | 303 | 5.12e-31 | 119 |
| MS.gene62423.t1 | MTR_1g030320 | 23.684 | 342 | 244 | 7 | 3 | 332 | 1 | 337 | 1.01e-27 | 111 |
| MS.gene62423.t1 | MTR_3g072380 | 23.708 | 329 | 239 | 6 | 11 | 332 | 12 | 335 | 2.66e-27 | 110 |
| MS.gene62423.t1 | MTR_4g018760 | 30.928 | 291 | 191 | 5 | 21 | 305 | 20 | 306 | 3.70e-27 | 109 |
| MS.gene62423.t1 | MTR_8g016090 | 27.326 | 344 | 235 | 9 | 8 | 338 | 163 | 504 | 2.48e-26 | 109 |
| MS.gene62423.t1 | MTR_4g101860 | 28.947 | 304 | 202 | 7 | 18 | 313 | 57 | 354 | 9.83e-25 | 103 |
| MS.gene62423.t1 | MTR_8g016090 | 28.302 | 265 | 179 | 6 | 84 | 338 | 191 | 454 | 6.30e-20 | 90.9 |
| MS.gene62423.t1 | MTR_4g058860 | 26.957 | 345 | 208 | 15 | 25 | 345 | 22 | 346 | 2.35e-18 | 85.1 |
| MS.gene62423.t1 | MTR_2g094640 | 25.449 | 334 | 213 | 13 | 25 | 344 | 22 | 333 | 5.46e-18 | 84.3 |
| MS.gene62423.t1 | MTR_3g094270 | 25.723 | 311 | 186 | 12 | 42 | 335 | 37 | 319 | 5.41e-15 | 75.5 |
| MS.gene62423.t1 | MTR_3g094260 | 25.161 | 310 | 189 | 10 | 42 | 335 | 37 | 319 | 4.57e-14 | 72.8 |
| MS.gene62423.t1 | MTR_3g072380 | 24.859 | 177 | 129 | 3 | 11 | 186 | 12 | 185 | 8.13e-13 | 67.8 |
| MS.gene62423.t1 | MTR_2g038410 | 24.768 | 323 | 217 | 9 | 28 | 345 | 69 | 370 | 2.82e-12 | 67.4 |
| MS.gene62423.t1 | MTR_8g016090 | 25.275 | 182 | 132 | 3 | 8 | 186 | 163 | 343 | 8.22e-12 | 66.2 |
| MS.gene62423.t1 | MTR_6g005480 | 24.920 | 313 | 215 | 8 | 4 | 305 | 104 | 407 | 2.44e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62423.t1 | AT5G25400 | 81.686 | 344 | 63 | 0 | 2 | 345 | 6 | 349 | 0.0 | 598 |
| MS.gene62423.t1 | AT4G32390 | 82.029 | 345 | 61 | 1 | 2 | 345 | 6 | 350 | 0.0 | 593 |
| MS.gene62423.t1 | AT2G25520 | 82.558 | 344 | 58 | 1 | 2 | 345 | 6 | 347 | 0.0 | 593 |
| MS.gene62423.t1 | AT5G11230 | 79.710 | 345 | 69 | 1 | 2 | 345 | 6 | 350 | 0.0 | 569 |
| MS.gene62423.t1 | AT3G17430 | 45.427 | 328 | 177 | 2 | 9 | 335 | 7 | 333 | 4.66e-98 | 295 |
| MS.gene62423.t1 | AT3G17430 | 45.427 | 328 | 177 | 2 | 9 | 335 | 7 | 333 | 4.66e-98 | 295 |
| MS.gene62423.t1 | AT1G48230 | 46.334 | 341 | 169 | 3 | 9 | 336 | 7 | 346 | 1.35e-96 | 291 |
| MS.gene62423.t1 | AT3G14410 | 42.771 | 332 | 187 | 3 | 4 | 333 | 5 | 335 | 1.28e-82 | 254 |
| MS.gene62423.t1 | AT1G53660 | 41.390 | 331 | 183 | 6 | 6 | 334 | 9 | 330 | 2.30e-82 | 253 |
| MS.gene62423.t1 | AT1G53660 | 41.390 | 331 | 183 | 6 | 6 | 334 | 27 | 348 | 4.49e-82 | 253 |
| MS.gene62423.t1 | AT1G53660 | 42.434 | 304 | 164 | 6 | 33 | 334 | 2 | 296 | 2.09e-78 | 242 |
| MS.gene62423.t1 | AT1G48230 | 44.891 | 274 | 137 | 3 | 76 | 336 | 1 | 273 | 1.91e-74 | 232 |
| MS.gene62423.t1 | AT1G53660 | 42.449 | 245 | 131 | 5 | 91 | 334 | 1 | 236 | 1.77e-58 | 189 |
| MS.gene62423.t1 | AT1G53660 | 41.294 | 201 | 117 | 1 | 5 | 204 | 26 | 226 | 6.01e-51 | 170 |
| MS.gene62423.t1 | AT1G21870 | 23.810 | 315 | 230 | 5 | 21 | 330 | 22 | 331 | 1.26e-30 | 119 |
| MS.gene62423.t1 | AT3G11320 | 31.757 | 296 | 188 | 5 | 18 | 305 | 15 | 304 | 1.58e-30 | 118 |
| MS.gene62423.t1 | AT5G05820 | 32.095 | 296 | 187 | 5 | 18 | 305 | 15 | 304 | 4.85e-30 | 117 |
| MS.gene62423.t1 | AT1G77610 | 24.383 | 324 | 232 | 6 | 21 | 336 | 16 | 334 | 4.41e-29 | 115 |
| MS.gene62423.t1 | AT3G10290 | 30.392 | 306 | 197 | 8 | 9 | 305 | 53 | 351 | 4.15e-27 | 110 |
| MS.gene62423.t1 | AT5G04160 | 30.255 | 314 | 199 | 8 | 3 | 305 | 1 | 305 | 2.82e-26 | 106 |
| MS.gene62423.t1 | AT1G12500 | 28.065 | 310 | 211 | 6 | 3 | 305 | 48 | 352 | 1.26e-23 | 100 |
| MS.gene62423.t1 | AT1G06470 | 25.000 | 336 | 232 | 9 | 17 | 335 | 78 | 410 | 6.48e-23 | 99.0 |
| MS.gene62423.t1 | AT1G06470 | 25.000 | 336 | 232 | 9 | 17 | 335 | 78 | 410 | 6.48e-23 | 99.0 |
| MS.gene62423.t1 | AT1G06470 | 25.000 | 336 | 232 | 9 | 17 | 335 | 78 | 410 | 6.48e-23 | 99.0 |
| MS.gene62423.t1 | AT1G06470 | 25.000 | 336 | 232 | 9 | 17 | 335 | 78 | 410 | 6.48e-23 | 99.0 |
| MS.gene62423.t1 | AT2G28315 | 27.187 | 320 | 199 | 13 | 25 | 333 | 22 | 318 | 7.64e-19 | 86.7 |
| MS.gene62423.t1 | AT2G28315 | 28.276 | 290 | 181 | 12 | 25 | 303 | 22 | 295 | 2.20e-17 | 82.0 |
| MS.gene62423.t1 | AT2G30460 | 27.941 | 272 | 165 | 9 | 83 | 336 | 73 | 331 | 9.14e-15 | 74.7 |
| MS.gene62423.t1 | AT2G30460 | 27.941 | 272 | 165 | 9 | 83 | 336 | 73 | 331 | 9.14e-15 | 74.7 |
| MS.gene62423.t1 | AT2G30460 | 27.941 | 272 | 165 | 9 | 83 | 336 | 52 | 310 | 9.74e-15 | 74.7 |
| MS.gene62423.t1 | AT2G30460 | 27.941 | 272 | 165 | 9 | 83 | 336 | 52 | 310 | 9.74e-15 | 74.7 |
| MS.gene62423.t1 | AT2G30460 | 27.941 | 272 | 165 | 9 | 83 | 336 | 52 | 310 | 9.74e-15 | 74.7 |
| MS.gene62423.t1 | AT1G06890 | 25.397 | 315 | 195 | 11 | 42 | 336 | 37 | 331 | 4.21e-13 | 70.1 |
| MS.gene62423.t1 | AT1G06890 | 25.397 | 315 | 195 | 11 | 42 | 336 | 37 | 331 | 4.31e-13 | 69.7 |
| MS.gene62423.t1 | AT1G06890 | 25.397 | 315 | 195 | 11 | 42 | 336 | 37 | 331 | 4.66e-13 | 69.7 |
| MS.gene62423.t1 | AT1G06890 | 25.397 | 315 | 195 | 11 | 42 | 336 | 37 | 331 | 4.66e-13 | 69.7 |
| MS.gene62423.t1 | AT5G33320 | 24.272 | 309 | 220 | 7 | 4 | 305 | 98 | 399 | 8.23e-11 | 63.2 |
Find 64 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTGTTACACTTCAGCTTA+TGG | 0.214354 | 8.2:+14207825 | MS.gene62423:CDS |
| CCGATTAATTTGATTGGTTA+TGG | 0.225210 | 8.2:+14208192 | MS.gene62423:CDS |
| GGTGTTGCTGTTGCTGCTTA+TGG | 0.242108 | 8.2:+14207781 | MS.gene62423:CDS |
| ATTCTCGTGGTCGGTGATTA+AGG | 0.249502 | 8.2:+14208158 | MS.gene62423:CDS |
| GTGTTGCTGTTGCTGCTTAT+GGG | 0.272371 | 8.2:+14207782 | MS.gene62423:CDS |
| CACTTTACTCGCTTTCGTTA+TGG | 0.286530 | 8.2:+14207608 | MS.gene62423:CDS |
| CGATTAATTTGATTGGTTAT+GGG | 0.330326 | 8.2:+14208193 | MS.gene62423:CDS |
| TTCGAAGTTGCAGGCTCTTA+AGG | 0.341248 | 8.2:+14208248 | MS.gene62423:CDS |
| GCTTTGACTATGAATGTTGC+TGG | 0.346970 | 8.2:+14208108 | MS.gene62423:CDS |
| TAGATAAATATACGCAGAAT+TGG | 0.353850 | 8.2:-14207635 | None:intergenic |
| GTTACACCGATTAATTTGAT+TGG | 0.366012 | 8.2:+14208186 | MS.gene62423:CDS |
| GGATAACTCAAGTTTCCATT+TGG | 0.385544 | 8.2:+14208005 | MS.gene62423:CDS |
| AAGAGAAATGGAAATCATAT+TGG | 0.391631 | 8.2:-14207758 | None:intergenic |
| TTCCGATGGCGTTGTGAAGA+AGG | 0.398342 | 8.2:+14207348 | MS.gene62423:CDS |
| GGGAAGCTAAATTTGATACT+TGG | 0.404187 | 8.2:+14207803 | MS.gene62423:CDS |
| TTATAATCATTCGAAGTTGC+AGG | 0.406175 | 8.2:+14208239 | MS.gene62423:CDS |
| GAATGTTGCTGGGGTTGTTA+AGG | 0.411142 | 8.2:+14208119 | MS.gene62423:CDS |
| AGATCGAGTTGAATCAGTTC+TGG | 0.413437 | 8.2:-14208368 | None:intergenic |
| AATATGATTTCCATTTCTCT+TGG | 0.417528 | 8.2:+14207760 | MS.gene62423:CDS |
| CATACACCTACGTCGCAATC+TGG | 0.421498 | 8.2:+14207380 | MS.gene62423:CDS |
| CCTGTTGCTGTTTATTCGAT+TGG | 0.427774 | 8.2:+14207694 | MS.gene62423:CDS |
| ATGTAATACAACGAAGTAAT+CGG | 0.430025 | 8.2:-14207919 | None:intergenic |
| AACAGCAACACCAAGAGAAA+TGG | 0.430794 | 8.2:-14207770 | None:intergenic |
| CCATAACCAATCAAATTAAT+CGG | 0.432294 | 8.2:-14208192 | None:intergenic |
| TTACATTGCGCCATGTTGTT+TGG | 0.446571 | 8.2:+14207936 | MS.gene62423:CDS |
| TGAATCATGGTTAGAGAGAT+CGG | 0.453928 | 8.2:-14207475 | None:intergenic |
| CTTTGACTATGAATGTTGCT+GGG | 0.457141 | 8.2:+14208109 | MS.gene62423:CDS |
| CTCAAATCTGTTGTTCCAAT+CGG | 0.464601 | 8.2:+14207583 | MS.gene62423:CDS |
| AGATCGCGTGACATTGAAAC+AGG | 0.469171 | 8.2:-14207556 | None:intergenic |
| GGAAGCTAAATTTGATACTT+GGG | 0.472356 | 8.2:+14207804 | MS.gene62423:CDS |
| TAGATCGTAAGATGTACAAT+TGG | 0.478800 | 8.2:+14207446 | MS.gene62423:CDS |
| TTGAGCCTTCTTCTGCGTCT+CGG | 0.479012 | 8.2:-14208274 | None:intergenic |
| CCAATCGAATAAACAGCAAC+AGG | 0.480862 | 8.2:-14207694 | None:intergenic |
| TAATTTGATTGGTTATGGGT+TGG | 0.488621 | 8.2:+14208197 | MS.gene62423:CDS |
| TTGCTGGGGTTGTTAAGGAT+TGG | 0.496578 | 8.2:+14208124 | MS.gene62423:CDS |
| GTATTTGTTGTAAACGATAA+CGG | 0.498137 | 8.2:-14207419 | None:intergenic |
| AGCGAGTAAAGTGCACCGAT+TGG | 0.499200 | 8.2:-14207598 | None:intergenic |
| TGAAGAGGCTGGAAGGTTGT+TGG | 0.507877 | 8.2:+14208305 | MS.gene62423:CDS |
| GGCTTCCGAGACGCAGAAGA+AGG | 0.521970 | 8.2:+14208269 | MS.gene62423:CDS |
| AGGTTGTTGGAACAGAGAGA+TGG | 0.527055 | 8.2:+14208318 | MS.gene62423:CDS |
| CAGAGAGATGGAGAAGGGAC+TGG | 0.531231 | 8.2:+14208330 | MS.gene62423:CDS |
| GAATCATGGTTAGAGAGATC+GGG | 0.533528 | 8.2:-14207474 | None:intergenic |
| CAACAGAGTGATGAAGAGGC+TGG | 0.548342 | 8.2:+14208294 | MS.gene62423:CDS |
| GTTGATTGCATTCTCGTGGT+CGG | 0.553496 | 8.2:+14208149 | MS.gene62423:CDS |
| TGTTGAGTATCCTTCGTTGA+GGG | 0.554979 | 8.2:+14207984 | MS.gene62423:CDS |
| GGCTCAACAGAGTGATGAAG+AGG | 0.559642 | 8.2:+14208290 | MS.gene62423:CDS |
| TTGTTGAGTATCCTTCGTTG+AGG | 0.563149 | 8.2:+14207983 | MS.gene62423:CDS |
| ATGGTTAGAGAGATCGGGTA+AGG | 0.564381 | 8.2:-14207469 | None:intergenic |
| TGTTGCTGTTGCTGCTTATG+GGG | 0.565614 | 8.2:+14207783 | MS.gene62423:CDS |
| GAGATGGAGAAGGGACTGGA+AGG | 0.571557 | 8.2:+14208334 | MS.gene62423:CDS |
| AACCTTCTTCACAACGCCAT+CGG | 0.572106 | 8.2:-14207350 | None:intergenic |
| CTCTCTAACCATGATTCACA+TGG | 0.588163 | 8.2:+14207480 | MS.gene62423:CDS |
| TTTGTTGGTTGGGAAGACGT+CGG | 0.592329 | 8.2:+14208086 | MS.gene62423:CDS |
| TTGGAACAGAGAGATGGAGA+AGG | 0.602800 | 8.2:+14208324 | MS.gene62423:CDS |
| AGAGTGATGAAGAGGCTGGA+AGG | 0.603959 | 8.2:+14208298 | MS.gene62423:CDS |
| GGTTGTTGATTGCATTCTCG+TGG | 0.604485 | 8.2:+14208145 | MS.gene62423:CDS |
| GAAGATCCAGATTGCGACGT+AGG | 0.612548 | 8.2:-14207386 | None:intergenic |
| GAAGCTAAATTTGATACTTG+GGG | 0.628264 | 8.2:+14207805 | MS.gene62423:CDS |
| TGGAACAGAGAGATGGAGAA+GGG | 0.630504 | 8.2:+14208325 | MS.gene62423:CDS |
| GGATACTCAACAATAAGCCA+AGG | 0.631347 | 8.2:-14207973 | None:intergenic |
| AAACGATAACGGTGAAGCTG+AGG | 0.632551 | 8.2:-14207408 | None:intergenic |
| TTGCGACGTAGGTGTATGAG+AGG | 0.648256 | 8.2:-14207375 | None:intergenic |
| TTTGACTATGAATGTTGCTG+GGG | 0.655153 | 8.2:+14208110 | MS.gene62423:CDS |
| CTTGAGTTATCCCTCAACGA+AGG | 0.699678 | 8.2:-14207994 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAGAAATGGAAATCATAT+TGG | - | chr8.2:14207761-14207780 | None:intergenic | 25.0% |
| ! | AATATGATTTCCATTTCTCT+TGG | + | chr8.2:14207760-14207779 | MS.gene62423:CDS | 25.0% |
| ! | ATGTAATACAACGAAGTAAT+CGG | - | chr8.2:14207922-14207941 | None:intergenic | 25.0% |
| ! | CCATAACCAATCAAATTAAT+CGG | - | chr8.2:14208195-14208214 | None:intergenic | 25.0% |
| ! | TAGATAAATATACGCAGAAT+TGG | - | chr8.2:14207638-14207657 | None:intergenic | 25.0% |
| !! | ATTTGGATTTCGCAATTTTT+GGG | + | chr8.2:14208022-14208041 | MS.gene62423:CDS | 25.0% |
| !! | CGATTAATTTGATTGGTTAT+GGG | + | chr8.2:14208193-14208212 | MS.gene62423:CDS | 25.0% |
| !!! | AGGTTTTAAGAATGAAACTA+TGG | + | chr8.2:14207735-14207754 | MS.gene62423:CDS | 25.0% |
| !!! | GTATTTGTTGTAAACGATAA+CGG | - | chr8.2:14207422-14207441 | None:intergenic | 25.0% |
| CAAAAATTGCGAAATCCAAA+TGG | - | chr8.2:14208023-14208042 | None:intergenic | 30.0% | |
| GAAGCTAAATTTGATACTTG+GGG | + | chr8.2:14207805-14207824 | MS.gene62423:CDS | 30.0% | |
| GGAAGCTAAATTTGATACTT+GGG | + | chr8.2:14207804-14207823 | MS.gene62423:CDS | 30.0% | |
| TAGATCGTAAGATGTACAAT+TGG | + | chr8.2:14207446-14207465 | MS.gene62423:CDS | 30.0% | |
| TTATAATCATTCGAAGTTGC+AGG | + | chr8.2:14208239-14208258 | MS.gene62423:CDS | 30.0% | |
| ! | AGATTTTGCTTAATTCGAAG+GGG | + | chr8.2:14207884-14207903 | MS.gene62423:CDS | 30.0% |
| ! | CAGATTTTGCTTAATTCGAA+GGG | + | chr8.2:14207883-14207902 | MS.gene62423:CDS | 30.0% |
| ! | CATTTGGATTTCGCAATTTT+TGG | + | chr8.2:14208021-14208040 | MS.gene62423:CDS | 30.0% |
| ! | CCGATTAATTTGATTGGTTA+TGG | + | chr8.2:14208192-14208211 | MS.gene62423:CDS | 30.0% |
| ! | GTTACACCGATTAATTTGAT+TGG | + | chr8.2:14208186-14208205 | MS.gene62423:CDS | 30.0% |
| ! | TAATTTGATTGGTTATGGGT+TGG | + | chr8.2:14208197-14208216 | MS.gene62423:CDS | 30.0% |
| ! | TCAGATTTTGCTTAATTCGA+AGG | + | chr8.2:14207882-14207901 | MS.gene62423:CDS | 30.0% |
| !!! | GGTGTTTTGTTTAAGAAAGA+AGG | + | chr8.2:14207715-14207734 | MS.gene62423:CDS | 30.0% |
| ACAAAAAGCCATGTGAATCA+TGG | - | chr8.2:14207491-14207510 | None:intergenic | 35.0% | |
| CTCAAATCTGTTGTTCCAAT+CGG | + | chr8.2:14207583-14207602 | MS.gene62423:CDS | 35.0% | |
| CTTTGACTATGAATGTTGCT+GGG | + | chr8.2:14208109-14208128 | MS.gene62423:CDS | 35.0% | |
| GACAAAAACACCAAACAACA+TGG | - | chr8.2:14207949-14207968 | None:intergenic | 35.0% | |
| GGATAACTCAAGTTTCCATT+TGG | + | chr8.2:14208005-14208024 | MS.gene62423:CDS | 35.0% | |
| GGGAAGCTAAATTTGATACT+TGG | + | chr8.2:14207803-14207822 | MS.gene62423:CDS | 35.0% | |
| TGAATCATGGTTAGAGAGAT+CGG | - | chr8.2:14207478-14207497 | None:intergenic | 35.0% | |
| TTTGACTATGAATGTTGCTG+GGG | + | chr8.2:14208110-14208129 | MS.gene62423:CDS | 35.0% | |
| AACAGCAACACCAAGAGAAA+TGG | - | chr8.2:14207773-14207792 | None:intergenic | 40.0% | |
| CACTTTACTCGCTTTCGTTA+TGG | + | chr8.2:14207608-14207627 | MS.gene62423:CDS | 40.0% | |
| CCAATCGAATAAACAGCAAC+AGG | - | chr8.2:14207697-14207716 | None:intergenic | 40.0% | |
| CTCTCTAACCATGATTCACA+TGG | + | chr8.2:14207480-14207499 | MS.gene62423:CDS | 40.0% | |
| CTTCCCAACCAACAAAAACA+CGG | - | chr8.2:14208082-14208101 | None:intergenic | 40.0% | |
| GAATCATGGTTAGAGAGATC+GGG | - | chr8.2:14207477-14207496 | None:intergenic | 40.0% | |
| GCTTTGACTATGAATGTTGC+TGG | + | chr8.2:14208108-14208127 | MS.gene62423:CDS | 40.0% | |
| GGATACTCAACAATAAGCCA+AGG | - | chr8.2:14207976-14207995 | None:intergenic | 40.0% | |
| TGTTGAGTATCCTTCGTTGA+GGG | + | chr8.2:14207984-14208003 | MS.gene62423:CDS | 40.0% | |
| TTACATTGCGCCATGTTGTT+TGG | + | chr8.2:14207936-14207955 | MS.gene62423:CDS | 40.0% | |
| TTGTTGAGTATCCTTCGTTG+AGG | + | chr8.2:14207983-14208002 | MS.gene62423:CDS | 40.0% | |
| !! | CCTGTTGCTGTTTATTCGAT+TGG | + | chr8.2:14207694-14207713 | MS.gene62423:CDS | 40.0% |
| !! | GAATCTTGCCGTGTTTTTGT+TGG | + | chr8.2:14208071-14208090 | MS.gene62423:CDS | 40.0% |
| !! | TGGTGTTTTTGTCTGTTCCT+TGG | + | chr8.2:14207956-14207975 | MS.gene62423:CDS | 40.0% |
| !! | TTGCCGTGTTTTTGTTGGTT+GGG | + | chr8.2:14208076-14208095 | MS.gene62423:CDS | 40.0% |
| !!! | TGCTTTTGAAGCTACACGTT+TGG | + | chr8.2:14207852-14207871 | MS.gene62423:CDS | 40.0% |
| AACCTTCTTCACAACGCCAT+CGG | - | chr8.2:14207353-14207372 | None:intergenic | 45.0% | |
| AGATCGCGTGACATTGAAAC+AGG | - | chr8.2:14207559-14207578 | None:intergenic | 45.0% | |
| AGGTTGTTGGAACAGAGAGA+TGG | + | chr8.2:14208318-14208337 | MS.gene62423:CDS | 45.0% | |
| ATGGTTAGAGAGATCGGGTA+AGG | - | chr8.2:14207472-14207491 | None:intergenic | 45.0% | |
| ATTCTCGTGGTCGGTGATTA+AGG | + | chr8.2:14208158-14208177 | MS.gene62423:CDS | 45.0% | |
| CTTGAGTTATCCCTCAACGA+AGG | - | chr8.2:14207997-14208016 | None:intergenic | 45.0% | |
| GAATGTTGCTGGGGTTGTTA+AGG | + | chr8.2:14208119-14208138 | MS.gene62423:CDS | 45.0% | |
| GTTGATTGCATTCTCGTGGT+CGG | + | chr8.2:14208149-14208168 | MS.gene62423:CDS | 45.0% | |
| TGGAACAGAGAGATGGAGAA+GGG | + | chr8.2:14208325-14208344 | MS.gene62423:CDS | 45.0% | |
| TTCGAAGTTGCAGGCTCTTA+AGG | + | chr8.2:14208248-14208267 | MS.gene62423:CDS | 45.0% | |
| TTGCTGGGGTTGTTAAGGAT+TGG | + | chr8.2:14208124-14208143 | MS.gene62423:CDS | 45.0% | |
| TTGGAACAGAGAGATGGAGA+AGG | + | chr8.2:14208324-14208343 | MS.gene62423:CDS | 45.0% | |
| ! | AAACGATAACGGTGAAGCTG+AGG | - | chr8.2:14207411-14207430 | None:intergenic | 45.0% |
| ! | GGGTGTTACACTTCAGCTTA+TGG | + | chr8.2:14207825-14207844 | MS.gene62423:CDS | 45.0% |
| ! | GGTTGTTGATTGCATTCTCG+TGG | + | chr8.2:14208145-14208164 | MS.gene62423:CDS | 45.0% |
| ! | GTGTTGCTGTTGCTGCTTAT+GGG | + | chr8.2:14207782-14207801 | MS.gene62423:CDS | 45.0% |
| ! | TGTTGCTGTTGCTGCTTATG+GGG | + | chr8.2:14207783-14207802 | MS.gene62423:CDS | 45.0% |
| ! | TTTGTTGGTTGGGAAGACGT+CGG | + | chr8.2:14208086-14208105 | MS.gene62423:CDS | 45.0% |
| !! | CTTGCCGTGTTTTTGTTGGT+TGG | + | chr8.2:14208075-14208094 | MS.gene62423:CDS | 45.0% |
| !!! | GGTTATGGGTTGGCGTTTTT+GGG | + | chr8.2:14208207-14208226 | MS.gene62423:CDS | 45.0% |
| !!! | GTTATGGGTTGGCGTTTTTG+GGG | + | chr8.2:14208208-14208227 | MS.gene62423:CDS | 45.0% |
| !!! | TGGTTATGGGTTGGCGTTTT+TGG | + | chr8.2:14208206-14208225 | MS.gene62423:CDS | 45.0% |
| !!! | TTATGGGTTGGCGTTTTTGG+GGG | + | chr8.2:14208209-14208228 | MS.gene62423:CDS | 45.0% |
| AGAGTGATGAAGAGGCTGGA+AGG | + | chr8.2:14208298-14208317 | MS.gene62423:CDS | 50.0% | |
| CAACAGAGTGATGAAGAGGC+TGG | + | chr8.2:14208294-14208313 | MS.gene62423:CDS | 50.0% | |
| CATACACCTACGTCGCAATC+TGG | + | chr8.2:14207380-14207399 | MS.gene62423:CDS | 50.0% | |
| GAAGATCCAGATTGCGACGT+AGG | - | chr8.2:14207389-14207408 | None:intergenic | 50.0% | |
| GGCTCAACAGAGTGATGAAG+AGG | + | chr8.2:14208290-14208309 | MS.gene62423:CDS | 50.0% | |
| TGAAGAGGCTGGAAGGTTGT+TGG | + | chr8.2:14208305-14208324 | MS.gene62423:CDS | 50.0% | |
| TTCCGATGGCGTTGTGAAGA+AGG | + | chr8.2:14207348-14207367 | MS.gene62423:CDS | 50.0% | |
| TTGAGCCTTCTTCTGCGTCT+CGG | - | chr8.2:14208277-14208296 | None:intergenic | 50.0% | |
| TTGCGACGTAGGTGTATGAG+AGG | - | chr8.2:14207378-14207397 | None:intergenic | 50.0% | |
| ! | AGCGAGTAAAGTGCACCGAT+TGG | - | chr8.2:14207601-14207620 | None:intergenic | 50.0% |
| !! | GGTGTTGCTGTTGCTGCTTA+TGG | + | chr8.2:14207781-14207800 | MS.gene62423:CDS | 50.0% |
| CAGAGAGATGGAGAAGGGAC+TGG | + | chr8.2:14208330-14208349 | MS.gene62423:CDS | 55.0% | |
| GAGATGGAGAAGGGACTGGA+AGG | + | chr8.2:14208334-14208353 | MS.gene62423:CDS | 55.0% | |
| GGCTTCCGAGACGCAGAAGA+AGG | + | chr8.2:14208269-14208288 | MS.gene62423:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 14207340 | 14208377 | 14207340 | ID=MS.gene62423 |
| chr8.2 | mRNA | 14207340 | 14208377 | 14207340 | ID=MS.gene62423.t1;Parent=MS.gene62423 |
| chr8.2 | exon | 14207340 | 14208377 | 14207340 | ID=MS.gene62423.t1.exon1;Parent=MS.gene62423.t1 |
| chr8.2 | CDS | 14207340 | 14208377 | 14207340 | ID=cds.MS.gene62423.t1;Parent=MS.gene62423.t1 |
| Gene Sequence |
| Protein sequence |
