AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88649


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88649.t1 MTR_1g077800 99.296 142 1 0 1 142 1 142 1.06e-100 302
MS.gene88649.t1 MTR_1g077800 99.296 142 1 0 1 142 1 142 1.17e-100 303
MS.gene88649.t1 MTR_6g006770 58.394 137 57 0 6 142 7 143 9.92e-56 184
MS.gene88649.t1 MTR_4g005730 56.296 135 59 0 8 142 17 151 3.42e-51 172
MS.gene88649.t1 MTR_4g005730 56.296 135 59 0 8 142 17 151 3.51e-51 172
MS.gene88649.t1 MTR_4g005730 56.296 135 59 0 8 142 17 151 4.27e-51 172
MS.gene88649.t1 MTR_2g100030 47.794 136 70 1 8 142 11 146 1.35e-43 150
MS.gene88649.t1 MTR_2g078740 40.625 128 74 1 15 142 15 140 1.26e-24 98.6
MS.gene88649.t1 MTR_2g078740 40.625 128 74 1 15 142 15 140 1.27e-24 98.6
MS.gene88649.t1 MTR_2g078740 40.625 128 74 1 15 142 15 140 1.36e-24 98.6
MS.gene88649.t1 MTR_5g045190 34.375 128 83 1 15 142 239 365 1.71e-19 84.0
MS.gene88649.t1 MTR_4g074875 30.233 129 89 1 15 142 20 148 1.01e-14 70.5
MS.gene88649.t1 MTR_4g074875 30.233 129 89 1 15 142 20 148 1.02e-14 70.5
MS.gene88649.t1 MTR_4g075410 30.233 129 89 1 15 142 20 148 1.90e-14 69.7
MS.gene88649.t1 MTR_4g075410 30.233 129 89 1 15 142 20 148 1.97e-14 69.3
MS.gene88649.t1 MTR_4g029020 30.657 137 90 2 6 140 3 136 5.25e-12 62.4
MS.gene88649.t1 MTR_5g075100 30.469 128 85 2 15 140 23 148 7.05e-12 62.0
MS.gene88649.t1 MTR_7g072575 29.231 130 87 2 13 140 10 136 1.00e-11 61.6
MS.gene88649.t1 MTR_2g020380 28.125 128 86 2 15 142 4 125 1.18e-11 61.6
MS.gene88649.t1 MTR_5g075060 26.923 130 90 2 13 140 10 136 2.02e-11 60.8
MS.gene88649.t1 MTR_7g097150 28.462 130 89 2 13 140 118 245 5.27e-11 59.7
MS.gene88649.t1 MTR_5g023150 28.767 146 88 5 4 142 187 323 7.57e-11 58.9
MS.gene88649.t1 MTR_5g023150 28.767 146 88 5 4 142 284 420 8.96e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88649.t1 AT4G10730 78.519 135 29 0 8 142 20 154 7.06e-76 238
MS.gene88649.t1 AT4G10730 78.519 135 29 0 8 142 40 174 7.89e-76 239
MS.gene88649.t1 AT4G24100 75.182 137 34 0 6 142 24 160 2.11e-73 232
MS.gene88649.t1 AT4G24100 75.182 137 34 0 6 142 40 176 2.74e-73 232
MS.gene88649.t1 AT4G24100 75.182 137 34 0 6 142 24 160 3.14e-73 232
MS.gene88649.t1 AT4G24100 75.182 137 34 0 6 142 40 176 3.56e-73 232
MS.gene88649.t1 AT5G14720 59.441 143 57 1 1 142 1 143 6.01e-57 188
MS.gene88649.t1 AT5G14720 59.441 143 57 1 1 142 1 143 6.01e-57 188
MS.gene88649.t1 AT1G79640 58.394 137 57 0 6 142 4 140 9.10e-54 179
MS.gene88649.t1 AT1G79640 58.394 137 57 0 6 142 7 143 1.16e-53 179
MS.gene88649.t1 AT1G79640 58.394 137 57 0 6 142 4 140 1.19e-53 179
MS.gene88649.t1 AT1G79640 58.394 137 57 0 6 142 4 140 1.19e-53 179
MS.gene88649.t1 AT1G79640 58.394 137 57 0 6 142 4 140 1.19e-53 179
MS.gene88649.t1 AT1G79640 58.394 137 57 0 6 142 4 140 1.25e-53 179
MS.gene88649.t1 AT1G70430 55.319 141 63 0 2 142 3 143 3.72e-52 174
MS.gene88649.t1 AT1G70430 55.319 141 63 0 2 142 3 143 4.23e-52 174
MS.gene88649.t1 AT1G70430 55.319 141 63 0 2 142 3 143 4.72e-52 174
MS.gene88649.t1 AT4G14480 44.056 143 79 1 1 142 1 143 5.50e-42 145
MS.gene88649.t1 AT1G23700 49.231 130 62 1 13 142 14 139 1.71e-39 137
MS.gene88649.t1 AT1G23700 49.231 130 62 1 13 142 14 139 5.93e-39 137
MS.gene88649.t1 AT1G23700 49.231 130 62 1 13 142 14 139 7.55e-39 136
MS.gene88649.t1 AT1G70430 60.256 78 31 0 65 142 1 78 1.45e-30 115
MS.gene88649.t1 AT3G15220 37.500 128 78 1 15 142 15 140 3.44e-19 83.2
MS.gene88649.t1 AT1G69220 35.156 128 82 1 15 142 249 375 1.28e-18 81.6
MS.gene88649.t1 AT1G53165 35.938 128 80 1 15 142 15 140 1.82e-18 80.9
MS.gene88649.t1 AT1G53165 35.938 128 80 1 15 142 15 140 1.84e-18 80.9
MS.gene88649.t1 AT1G53165 35.938 128 80 1 15 142 15 140 1.85e-18 80.9
MS.gene88649.t1 AT1G69220 32.558 129 86 1 14 142 221 348 2.12e-15 72.4
MS.gene88649.t1 AT3G13530 30.233 129 89 1 15 142 20 148 3.13e-14 68.9
MS.gene88649.t1 AT3G07980 30.233 129 89 1 15 142 20 148 4.70e-14 68.6
MS.gene88649.t1 AT3G07980 30.233 129 89 1 15 142 20 148 5.07e-14 68.6
MS.gene88649.t1 AT3G21220 28.800 125 83 2 18 142 73 191 6.76e-12 62.0
MS.gene88649.t1 AT3G21220 28.800 125 83 2 18 142 73 191 6.76e-12 62.0
MS.gene88649.t1 AT1G50240 30.233 129 86 3 13 140 4 129 1.85e-11 60.8
MS.gene88649.t1 AT1G50240 30.233 129 86 3 13 140 4 129 1.85e-11 60.8
MS.gene88649.t1 AT5G66210 29.197 137 93 3 8 140 54 190 3.76e-11 60.1
MS.gene88649.t1 AT5G66210 29.197 137 93 3 8 140 54 190 3.76e-11 60.1
MS.gene88649.t1 AT5G66210 29.197 137 93 3 8 140 54 190 4.70e-11 59.7
MS.gene88649.t1 AT5G66210 29.197 137 93 3 8 140 54 190 4.75e-11 59.7
MS.gene88649.t1 AT5G66210 29.197 137 93 3 8 140 54 190 4.75e-11 59.7
MS.gene88649.t1 AT5G66210 29.197 137 93 3 8 140 54 190 5.16e-11 59.7
MS.gene88649.t1 AT4G35780 29.452 146 87 5 4 142 281 417 5.52e-11 59.7
MS.gene88649.t1 AT3G12690 32.857 140 86 4 9 141 175 313 6.03e-11 59.3
MS.gene88649.t1 AT3G12690 32.857 140 86 4 9 141 175 313 6.03e-11 59.3
MS.gene88649.t1 AT3G12690 32.857 140 86 4 9 141 175 313 6.03e-11 59.3
MS.gene88649.t1 AT1G63700 30.508 118 72 3 33 142 414 529 6.82e-11 59.3
MS.gene88649.t1 AT1G63700 30.508 118 72 3 33 142 414 529 6.82e-11 59.3

Find 19 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAACGTCTCTTTCAAAA+TGG 0.265015 1.2:+62855443 None:intergenic
CATGAACCTTGTGCCATAAA+AGG 0.291875 1.2:+62855522 None:intergenic
CGTTGTGGATCGTAAACTTT+GGG 0.347503 1.2:-62855553 MS.gene88649:CDS
ACCGCTACTTCTTCGTTGTA+TGG 0.392284 1.2:+62855687 None:intergenic
GAAAGAGACGTTGAAAGCTT+TGG 0.396603 1.2:-62855436 MS.gene88649:CDS
GGATTTGAAGAAGATGCTAT+TGG 0.418626 1.2:-62855468 MS.gene88649:CDS
ACACATCACTAAATGGGTTC+AGG 0.447322 1.2:-62855804 None:intergenic
TACAAGCTTCTAGAAGAAGT+TGG 0.486254 1.2:-62855750 MS.gene88649:CDS
ATGAAATCAGCTTATCCTGA+TGG 0.487247 1.2:-62855489 MS.gene88649:CDS
TCGTTGTGGATCGTAAACTT+TGG 0.492637 1.2:-62855554 MS.gene88649:CDS
CTTCTAGAAGAAGTTGGTTA+CGG 0.506829 1.2:-62855744 MS.gene88649:CDS
GCATCTTCTTCAAATCCATC+AGG 0.515627 1.2:+62855474 None:intergenic
TCTAGAAGCTTGTAATCTTG+AGG 0.521214 1.2:+62855759 None:intergenic
ATCGTATTGTTCCTTCGTTG+TGG 0.548419 1.2:-62855568 MS.gene88649:CDS
ATTCATCGTGATGTTAAAGC+TGG 0.566678 1.2:-62855390 MS.gene88649:intron
TTGGATTATCTTCATCAACA+TGG 0.602787 1.2:-62855417 MS.gene88649:CDS
TGGTTACGGTGCAAGCGCAA+CGG 0.606517 1.2:-62855730 MS.gene88649:CDS
TCCATACAACGAAGAAGTAG+CGG 0.655481 1.2:-62855688 MS.gene88649:CDS
AAGTTTACGATCCACAACGA+AGG 0.725773 1.2:+62855557 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTTTGCTTGATTCTAA+TGG - chr1.2:62855723-62855742 MS.gene88649:CDS 20.0%
TAATGGTGAAGTCAAACTTT+CGG - chr1.2:62855740-62855759 MS.gene88649:CDS 30.0%
TTGGATTATCTTCATCAACA+TGG - chr1.2:62855672-62855691 MS.gene88649:CDS 30.0%
TTTCAACGTCTCTTTCAAAA+TGG + chr1.2:62855649-62855668 None:intergenic 30.0%
ATGAAATCAGCTTATCCTGA+TGG - chr1.2:62855600-62855619 MS.gene88649:CDS 35.0%
ATTCATCGTGATGTTAAAGC+TGG - chr1.2:62855699-62855718 MS.gene88649:CDS 35.0%
GGATTTGAAGAAGATGCTAT+TGG - chr1.2:62855621-62855640 MS.gene88649:CDS 35.0%
TCTAGAAGCTTGTAATCTTG+AGG + chr1.2:62855333-62855352 None:intergenic 35.0%
TCTGCTTCTATGTTTGATAC+TGG - chr1.2:62855774-62855793 MS.gene88649:CDS 35.0%
! ATTATGCCTTTTATGGCACA+AGG - chr1.2:62855561-62855580 MS.gene88649:CDS 35.0%
! CTTCTAGAAGAAGTTGGTTA+CGG - chr1.2:62855345-62855364 MS.gene88649:intron 35.0%
! GAAGTCAAACTTTCGGATTT+TGG - chr1.2:62855747-62855766 MS.gene88649:CDS 35.0%
! TACAAGCTTCTAGAAGAAGT+TGG - chr1.2:62855339-62855358 MS.gene88649:intron 35.0%
! TTGGGTGATTATGCCTTTTA+TGG - chr1.2:62855554-62855573 MS.gene88649:CDS 35.0%
AAGTTTACGATCCACAACGA+AGG + chr1.2:62855535-62855554 None:intergenic 40.0%
ATCGTATTGTTCCTTCGTTG+TGG - chr1.2:62855521-62855540 MS.gene88649:CDS 40.0%
CATGAACCTTGTGCCATAAA+AGG + chr1.2:62855570-62855589 None:intergenic 40.0%
CGTTGTGGATCGTAAACTTT+GGG - chr1.2:62855536-62855555 MS.gene88649:CDS 40.0%
GAAAGAGACGTTGAAAGCTT+TGG - chr1.2:62855653-62855672 MS.gene88649:CDS 40.0%
GCATCTTCTTCAAATCCATC+AGG + chr1.2:62855618-62855637 None:intergenic 40.0%
TCCATACAACGAAGAAGTAG+CGG - chr1.2:62855401-62855420 MS.gene88649:CDS 40.0%
TCGTTGTGGATCGTAAACTT+TGG - chr1.2:62855535-62855554 MS.gene88649:CDS 40.0%
ACCGCTACTTCTTCGTTGTA+TGG + chr1.2:62855405-62855424 None:intergenic 45.0%
TGGTTACGGTGCAAGCGCAA+CGG - chr1.2:62855359-62855378 MS.gene88649:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 62855297 62855814 62855297 ID=MS.gene88649
chr1.2 mRNA 62855297 62855814 62855297 ID=MS.gene88649.t1;Parent=MS.gene88649
chr1.2 exon 62855391 62855814 62855391 ID=MS.gene88649.t1.exon1;Parent=MS.gene88649.t1
chr1.2 CDS 62855391 62855814 62855391 ID=cds.MS.gene88649.t1;Parent=MS.gene88649.t1
chr1.2 exon 62855297 62855304 62855297 ID=MS.gene88649.t1.exon2;Parent=MS.gene88649.t1
chr1.2 CDS 62855297 62855304 62855297 ID=cds.MS.gene88649.t1;Parent=MS.gene88649.t1
Gene Sequence

>MS.gene88649

ATGGGTTCAGGAAGCAGAAGCTACTCAGCGAATCCTCAAGATTACAAGCTTCTAGAAGAAGTTGGTTACGGTGCAAGCGCAACGGTTTATCATGCAGTTTATCTTCCATACAACGAAGAAGTAGCGGTGAAATCTCTCGATCTAGATCGATGCAATTCAAATTTCGATGATATTCGTAAAGAAGCACAGATCATGAGCTTAATTGATCATCGAAACGTTGTGAAATCGTATTGTTCCTTCGTTGTGGATCGTAAACTTTGGGTGATTATGCCTTTTATGGCACAAGGTTCATGTTTGCATTTGATGAAATCAGCTTATCCTGATGGATTTGAAGAAGATGCTATTGGATCCATTTTGAAAGAGACGTTGAAAGCTTTGGATTATCTTCATCAACATGGACACATTCATCGTGATGTTAAAGCTGGTAATATTTTGCTTGATTCTAATGGTGAAGTCAAACTTTCGGATTTTGGTGTTTCTGCTTCTATGTTTGATACTGGTGATCGTCAGCGTTCTAG

Protein sequence

>MS.gene88649.t1

MGSGSRSYSANPQDYKLLEEVGYGASATVYHAVYLPYNEEVAVKSLDLDRCNSNFDDIRKEAQIMSLIDHRNVVKSYCSFVVDRKLWVIMPFMAQGSCLHLMKSAYPDGFEEDAIGSILKETLKALDYLHQHGHIHRDVKAAF