AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84840.t1 MTR_8g030550 99.206 126 1 0 1 126 1022 1147 1.49e-86 275
MS.gene84840.t1 MTR_8g030550 99.206 126 1 0 1 126 1022 1147 1.50e-86 275
MS.gene84840.t1 MTR_8g030550 99.206 126 1 0 1 126 1022 1147 1.51e-86 275
MS.gene84840.t1 MTR_7g078090 87.402 127 15 1 1 126 1029 1155 2.42e-76 245
MS.gene84840.t1 MTR_4g118720 50.388 129 61 2 1 126 1040 1168 1.88e-37 134
MS.gene84840.t1 MTR_5g005840 48.438 128 64 2 1 126 398 525 1.56e-36 132
MS.gene84840.t1 MTR_1g105050 49.219 128 63 2 1 126 411 538 7.54e-36 129
MS.gene84840.t1 MTR_4g118845 34.646 127 82 1 1 126 223 349 2.34e-28 108
MS.gene84840.t1 MTR_4g118845 34.646 127 82 1 1 126 36 162 2.57e-28 108
MS.gene84840.t1 MTR_2g020000 36.220 127 80 1 1 126 226 352 3.91e-27 105
MS.gene84840.t1 MTR_3g053910 36.090 133 77 2 2 126 607 739 2.53e-26 102
MS.gene84840.t1 MTR_3g053910 36.090 133 77 2 2 126 636 768 2.96e-26 102
MS.gene84840.t1 MTR_5g090090 36.567 134 75 2 2 126 653 785 2.90e-25 99.8
MS.gene84840.t1 MTR_5g090090 36.567 134 75 2 2 126 653 785 3.00e-25 99.8
MS.gene84840.t1 MTR_7g090960 36.090 133 78 1 1 126 532 664 5.70e-25 99.0
MS.gene84840.t1 MTR_7g090960 36.090 133 78 1 1 126 613 745 5.89e-25 99.0
MS.gene84840.t1 MTR_1g053955 39.370 127 76 1 1 126 556 682 7.17e-25 98.6
MS.gene84840.t1 MTR_7g039320 39.855 138 71 2 1 126 74 211 1.16e-24 97.8
MS.gene84840.t1 MTR_7g039320 39.855 138 71 2 1 126 74 211 1.30e-24 97.8
MS.gene84840.t1 MTR_7g045505 40.288 139 69 3 1 126 25 162 1.49e-24 97.8
MS.gene84840.t1 MTR_7g045505 40.288 139 69 3 1 126 25 162 1.64e-24 97.4
MS.gene84840.t1 MTR_1g013190 30.872 149 77 2 2 126 322 468 1.96e-23 94.4
MS.gene84840.t1 MTR_3g106210 31.973 147 78 1 2 126 324 470 4.61e-22 90.5
MS.gene84840.t1 MTR_3g106210 31.973 147 78 1 2 126 324 470 4.64e-22 90.5
MS.gene84840.t1 MTR_3g106210 31.973 147 78 1 2 126 324 470 4.64e-22 90.5
MS.gene84840.t1 MTR_3g106210 31.973 147 78 1 2 126 324 470 4.75e-22 90.5
MS.gene84840.t1 MTR_4g096930 36.232 138 73 3 3 126 771 907 3.19e-20 85.5
MS.gene84840.t1 MTR_5g020000 35.772 123 74 2 9 126 226 348 5.04e-20 84.7
MS.gene84840.t1 MTR_1g035330 33.333 138 74 4 5 126 235 370 2.15e-16 74.3
MS.gene84840.t1 MTR_4g078495 33.333 111 70 2 17 126 192 299 2.30e-14 68.6
MS.gene84840.t1 MTR_4g078460 33.333 111 70 2 17 126 478 585 2.37e-14 68.6
MS.gene84840.t1 MTR_4g078460 33.333 111 70 2 17 126 478 585 2.59e-14 68.6
MS.gene84840.t1 MTR_4g035100 31.250 112 73 2 17 126 1507 1616 8.04e-13 64.3
MS.gene84840.t1 MTR_4g035100 31.250 112 73 2 17 126 1507 1616 8.12e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84840.t1 AT2G46020 88.095 126 15 0 1 126 1016 1141 1.79e-76 246
MS.gene84840.t1 AT2G46020 88.095 126 15 0 1 126 1016 1141 1.79e-76 246
MS.gene84840.t1 AT2G46020 88.095 126 15 0 1 126 1016 1141 1.79e-76 246
MS.gene84840.t1 AT2G46020 88.189 127 14 1 1 126 1016 1142 2.28e-76 246
MS.gene84840.t1 AT2G46020 88.189 127 14 1 1 126 1016 1142 2.28e-76 246
MS.gene84840.t1 AT2G46020 88.189 127 14 1 1 126 1016 1142 2.28e-76 246
MS.gene84840.t1 AT5G19310 49.606 127 63 1 1 126 157 283 2.89e-39 139
MS.gene84840.t1 AT5G19310 49.606 127 63 1 1 126 421 547 3.82e-39 139
MS.gene84840.t1 AT2G28290 52.713 129 58 2 1 126 789 917 2.55e-38 137
MS.gene84840.t1 AT2G28290 52.713 129 58 2 1 126 789 917 2.55e-38 137
MS.gene84840.t1 AT2G28290 52.713 129 58 2 1 126 789 917 2.55e-38 137
MS.gene84840.t1 AT2G28290 52.713 129 58 2 1 126 790 918 2.55e-38 137
MS.gene84840.t1 AT2G28290 52.713 129 58 2 1 126 789 917 2.55e-38 137
MS.gene84840.t1 AT2G28290 52.713 129 58 2 1 126 790 918 2.55e-38 137
MS.gene84840.t1 AT3G06010 46.094 128 67 2 1 126 438 565 9.15e-34 124
MS.gene84840.t1 AT5G18620 33.858 127 83 1 1 126 229 355 1.52e-26 103
MS.gene84840.t1 AT5G18620 33.858 127 83 1 1 126 229 355 1.52e-26 103
MS.gene84840.t1 AT3G06400 33.858 127 83 1 1 126 224 350 3.23e-26 102
MS.gene84840.t1 AT3G06400 33.858 127 83 1 1 126 224 350 3.26e-26 102
MS.gene84840.t1 AT3G06400 33.858 127 83 1 1 126 224 350 3.26e-26 102
MS.gene84840.t1 AT3G12810 40.625 128 73 2 1 126 571 697 7.73e-25 98.6
MS.gene84840.t1 AT3G12810 40.625 128 73 2 1 126 567 693 8.11e-25 98.6
MS.gene84840.t1 AT3G57300 34.586 133 80 1 1 126 621 753 1.86e-24 97.4
MS.gene84840.t1 AT3G57300 34.586 133 80 1 1 126 621 753 2.09e-24 97.4
MS.gene84840.t1 AT3G57300 34.586 133 80 1 1 126 621 753 2.09e-24 97.4
MS.gene84840.t1 AT2G13370 35.338 133 78 2 2 126 661 793 3.92e-24 96.7
MS.gene84840.t1 AT2G13370 35.338 133 78 2 2 126 661 793 3.92e-24 96.7
MS.gene84840.t1 AT2G13370 35.338 133 78 2 2 126 661 793 3.92e-24 96.7
MS.gene84840.t1 AT5G66750 37.594 133 76 3 1 126 234 366 7.05e-24 95.9
MS.gene84840.t1 AT2G44980 38.168 131 76 2 1 126 87 217 1.67e-23 94.7
MS.gene84840.t1 AT2G44980 37.226 137 75 3 1 126 87 223 2.09e-22 91.7
MS.gene84840.t1 AT2G44980 37.226 137 75 3 1 126 86 222 2.30e-22 91.7
MS.gene84840.t1 AT2G25170 34.228 149 72 3 2 126 326 472 4.41e-22 90.9
MS.gene84840.t1 AT2G25170 34.228 149 72 3 2 126 335 481 4.50e-22 90.5
MS.gene84840.t1 AT2G25170 34.228 149 72 3 2 126 309 455 4.91e-22 90.5
MS.gene84840.t1 AT2G25170 34.228 149 72 3 2 126 309 455 4.91e-22 90.5
MS.gene84840.t1 AT5G44800 38.211 123 63 2 17 126 740 862 7.03e-22 90.1
MS.gene84840.t1 AT4G31900 36.434 129 75 2 5 126 220 348 1.03e-21 89.7
MS.gene84840.t1 AT4G31900 36.434 129 75 2 5 126 261 389 1.10e-21 89.7
MS.gene84840.t1 AT4G31900 36.434 129 75 2 5 126 261 389 1.25e-21 89.4
MS.gene84840.t1 AT3G57300 27.711 166 80 1 1 126 621 786 5.09e-20 84.7
MS.gene84840.t1 AT2G02090 31.884 138 76 4 5 126 253 388 1.31e-15 72.0
MS.gene84840.t1 AT1G03750 31.034 116 78 1 11 126 194 307 4.21e-14 67.8
MS.gene84840.t1 AT3G54280 29.464 112 75 2 17 126 1539 1648 3.39e-13 65.5
MS.gene84840.t1 AT3G54280 29.464 112 75 2 17 126 1508 1617 3.59e-13 65.1

Find 16 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTTAACCATGACAGCATT+TGG 0.335190 8.1:-72473674 None:intergenic
TTTGACAGGAACGCCGTTGC+AGG 0.361434 8.1:+72474290 MS.gene84840:CDS
TGCTCTGAAATTTAATGTCT+TGG 0.364924 8.1:+72474116 MS.gene84840:CDS
CAAATGCTGTCATGGTTAAC+TGG 0.373905 8.1:+72473675 MS.gene84840:CDS
GGCATTGATTGCATACTTGA+TGG 0.376025 8.1:+72473613 MS.gene84840:CDS
ATGGAATTCAAAGGAAACTA+TGG 0.408379 8.1:+72473632 MS.gene84840:CDS
AGTTAACCATGACAGCATTT+GGG 0.429148 8.1:-72473673 None:intergenic
TACAGAGTGAGTTATATAAG+TGG 0.529773 8.1:+72473778 MS.gene84840:intron
TGATGAAGCACAAAGAATGA+AGG 0.548822 8.1:+72474209 MS.gene84840:CDS
TTTGGGACTATTATGAGATG+TGG 0.559813 8.1:-72473656 None:intergenic
AAAAGAAAGCAGACCTGCAA+CGG 0.560812 8.1:-72474303 None:intergenic
AATAGTCCCAAATGCTGTCA+TGG 0.561516 8.1:+72473667 MS.gene84840:CDS
ATGAGTTCATCATGTATGAC+CGG 0.569436 8.1:+72474148 MS.gene84840:CDS
GCATACTTGATGGAATTCAA+AGG 0.579933 8.1:+72473623 MS.gene84840:CDS
TAAAAGATGCACGACACAGA+TGG 0.614890 8.1:-72473804 None:intergenic
TGCTGTCATGGTTAACTGGA+AGG 0.620544 8.1:+72473679 MS.gene84840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAATAAATCAAATCAAAT+AGG + chr8.1:72473973-72473992 MS.gene84840:intron 15.0%
!! ATGTTAATGTAGAAAGTAAT+TGG + chr8.1:72473896-72473915 MS.gene84840:intron 20.0%
!! CAAAATTATACTTTAGTTGT+AGG - chr8.1:72474052-72474071 None:intergenic 20.0%
!!! ATAAGAAAGCATAAAATCAA+AGG + chr8.1:72474001-72474020 MS.gene84840:intron 20.0%
! CAAAGCTTTCAAAAATTGAT+TGG + chr8.1:72474172-72474191 MS.gene84840:CDS 25.0%
! CCTAATATTTATATGAACAG+AGG - chr8.1:72473943-72473962 None:intergenic 25.0%
! CCTCTGTTCATATAAATATT+AGG + chr8.1:72473940-72473959 MS.gene84840:intron 25.0%
! TAAATGCACAAAAGAAGTTT+AGG - chr8.1:72474085-72474104 None:intergenic 25.0%
!! TTCTTTTGTGCATTTACAAT+AGG + chr8.1:72474089-72474108 MS.gene84840:intron 25.0%
ATGGAATTCAAAGGAAACTA+TGG + chr8.1:72473632-72473651 MS.gene84840:CDS 30.0%
TACAGAGTGAGTTATATAAG+TGG + chr8.1:72473778-72473797 MS.gene84840:intron 30.0%
!! CAATTTTTGAAAGCTTTGAC+CGG - chr8.1:72474170-72474189 None:intergenic 30.0%
!! TGCTCTGAAATTTAATGTCT+TGG + chr8.1:72474116-72474135 MS.gene84840:CDS 30.0%
ACTTTAGTTGTAGGAGAATC+AGG - chr8.1:72474043-72474062 None:intergenic 35.0%
AGTTAACCATGACAGCATTT+GGG - chr8.1:72473676-72473695 None:intergenic 35.0%
ATGAGTTCATCATGTATGAC+CGG + chr8.1:72474148-72474167 MS.gene84840:CDS 35.0%
GCATACTTGATGGAATTCAA+AGG + chr8.1:72473623-72473642 MS.gene84840:CDS 35.0%
TGATGAAGCACAAAGAATGA+AGG + chr8.1:72474209-72474228 MS.gene84840:CDS 35.0%
! TTTGGGACTATTATGAGATG+TGG - chr8.1:72473659-72473678 None:intergenic 35.0%
!!! TTTTAGCTCGTGATCTTGAT+AGG + chr8.1:72474244-72474263 MS.gene84840:CDS 35.0%
AATAGTCCCAAATGCTGTCA+TGG + chr8.1:72473667-72473686 MS.gene84840:CDS 40.0%
CAAATGCTGTCATGGTTAAC+TGG + chr8.1:72473675-72473694 MS.gene84840:CDS 40.0%
CAGTTAACCATGACAGCATT+TGG - chr8.1:72473677-72473696 None:intergenic 40.0%
TAAAAGATGCACGACACAGA+TGG - chr8.1:72473807-72473826 None:intergenic 40.0%
TCATCAAGTAAGTGTGCATC+AGG + chr8.1:72473860-72473879 MS.gene84840:intron 40.0%
!! CAAAGACGTCTACTTTTGAC+AGG + chr8.1:72474276-72474295 MS.gene84840:CDS 40.0%
!! GGCATTGATTGCATACTTGA+TGG + chr8.1:72473613-72473632 MS.gene84840:CDS 40.0%
TGCTGTCATGGTTAACTGGA+AGG + chr8.1:72473679-72473698 MS.gene84840:CDS 45.0%
! CATCTTTTATGCTGGAGGGA+AGG + chr8.1:72473818-72473837 MS.gene84840:CDS 45.0%
! CGTGCATCTTTTATGCTGGA+GGG + chr8.1:72473814-72473833 MS.gene84840:CDS 45.0%
! GTGTCGTGCATCTTTTATGC+TGG + chr8.1:72473810-72473829 MS.gene84840:CDS 45.0%
! TCGTGCATCTTTTATGCTGG+AGG + chr8.1:72473813-72473832 MS.gene84840:CDS 45.0%
TTTGACAGGAACGCCGTTGC+AGG + chr8.1:72474290-72474309 MS.gene84840:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 72473608 72474311 72473608 ID=MS.gene84840
chr8.1 mRNA 72473608 72474311 72473608 ID=MS.gene84840.t1;Parent=MS.gene84840
chr8.1 exon 72473608 72473700 72473608 ID=MS.gene84840.t1.exon1;Parent=MS.gene84840.t1
chr8.1 CDS 72473608 72473700 72473608 ID=cds.MS.gene84840.t1;Parent=MS.gene84840.t1
chr8.1 exon 72473783 72473866 72473783 ID=MS.gene84840.t1.exon2;Parent=MS.gene84840.t1
chr8.1 CDS 72473783 72473866 72473783 ID=cds.MS.gene84840.t1;Parent=MS.gene84840.t1
chr8.1 exon 72474111 72474311 72474111 ID=MS.gene84840.t1.exon3;Parent=MS.gene84840.t1
chr8.1 CDS 72474111 72474311 72474111 ID=cds.MS.gene84840.t1;Parent=MS.gene84840.t1
Gene Sequence

>MS.gene84840

GTTATGGCATTGATTGCATACTTGATGGAATTCAAAGGAAACTATGGTCCACATCTCATAATAGTCCCAAATGCTGTCATGGTTAACTGGAAGGTGAGTTTTTAGACTAACTCATGTGATTAATATTATTATTTTTGTAATTGTTTTGACAAGAGAAACTATCTTTATTGTACAGAGTGAGTTATATAAGTGGTTGCCATCTGTGTCGTGCATCTTTTATGCTGGAGGGAAGGATTATCGAACAAAATTATTTCATCAAGTAAGTGTGCATCAGGTTATTCATATAAAATGTTAATGTAGAAAGTAATTGGAAAATACTTTCATGTTTATTACCTCTGTTCATATAAATATTAGGACTAATAAACTGAAATAAATCAAATCAAATAGGACTAAATAAGAAAGCATAAAATCAAAGGAAAATATTACATATCTCCTGATTCTCCTACAACTAAAGTATAATTTTGTTAACTACTGCCTAAACTTCTTTTGTGCATTTACAATAGGTTTCTGCTCTGAAATTTAATGTCTTGGTGACTACTTATGAGTTCATCATGTATGACCGGTCAAAGCTTTCAAAAATTGATTGGAAGTATATCGTGATTGATGAAGCACAAAGAATGAAGGATAGAGATTCAGTTTTAGCTCGTGATCTTGATAGGTATCGTTGTCAAAGACGTCTACTTTTGACAGGAACGCCGTTGCAG

Protein sequence

>MS.gene84840.t1

VMALIAYLMEFKGNYGPHLIIVPNAVMVNWKSELYKWLPSVSCIFYAGGKDYRTKLFHQVSALKFNVLVTTYEFIMYDRSKLSKIDWKYIVIDEAQRMKDRDSVLARDLDRYRCQRRLLLTGTPLQ