Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37086.t1 | KEH20777.1 | 96.1 | 330 | 9 | 1 | 1 | 330 | 1 | 326 | 2.80E-173 | 617.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37086.t1 | Q8L936 | 49.6 | 343 | 148 | 8 | 8 | 328 | 9 | 348 | 1.8e-72 | 274.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37086.t1 | A0A072TTL4 | 96.1 | 330 | 9 | 1 | 1 | 330 | 1 | 326 | 2.0e-173 | 617.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene37086 | 0.821938 | 3.09E-53 | -1.69E-46 |
| MS.gene049254 | MS.gene37086 | 0.831446 | 1.66E-55 | -1.69E-46 |
| MS.gene049419 | MS.gene37086 | -0.805846 | 1.10E-49 | -1.69E-46 |
| MS.gene049452 | MS.gene37086 | 0.869458 | 3.05E-66 | -1.69E-46 |
| MS.gene049669 | MS.gene37086 | 0.821033 | 5.01E-53 | -1.69E-46 |
| MS.gene049958 | MS.gene37086 | 0.835391 | 1.72E-56 | -1.69E-46 |
| MS.gene049983 | MS.gene37086 | 0.809492 | 1.84E-50 | -1.69E-46 |
| MS.gene050026 | MS.gene37086 | 0.807133 | 5.87E-50 | -1.69E-46 |
| MS.gene050073 | MS.gene37086 | 0.800041 | 1.74E-48 | -1.69E-46 |
| MS.gene050105 | MS.gene37086 | 0.836486 | 9.10E-57 | -1.69E-46 |
| MS.gene050273 | MS.gene37086 | 0.80389 | 2.81E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene37086 | 0.827689 | 1.36E-54 | -1.69E-46 |
| MS.gene050680 | MS.gene37086 | 0.819594 | 1.07E-52 | -1.69E-46 |
| MS.gene050750 | MS.gene37086 | 0.826574 | 2.52E-54 | -1.69E-46 |
| MS.gene050759 | MS.gene37086 | 0.859772 | 3.28E-63 | -1.69E-46 |
| MS.gene050826 | MS.gene37086 | 0.849639 | 2.84E-60 | -1.69E-46 |
| MS.gene050953 | MS.gene37086 | 0.829078 | 6.30E-55 | -1.69E-46 |
| MS.gene050962 | MS.gene37086 | 0.85402 | 1.62E-61 | -1.69E-46 |
| MS.gene051122 | MS.gene37086 | 0.814192 | 1.75E-51 | -1.69E-46 |
| MS.gene051190 | MS.gene37086 | 0.820942 | 5.25E-53 | -1.69E-46 |
| MS.gene051198 | MS.gene37086 | 0.801071 | 1.07E-48 | -1.69E-46 |
| MS.gene051279 | MS.gene37086 | 0.816757 | 4.69E-52 | -1.69E-46 |
| MS.gene051332 | MS.gene37086 | 0.803631 | 3.18E-49 | -1.69E-46 |
| MS.gene051368 | MS.gene37086 | 0.846816 | 1.71E-59 | -1.69E-46 |
| MS.gene051490 | MS.gene37086 | 0.810184 | 1.31E-50 | -1.69E-46 |
| MS.gene051800 | MS.gene37086 | 0.815908 | 7.26E-52 | -1.69E-46 |
| MS.gene051924 | MS.gene37086 | -0.841586 | 4.35E-58 | -1.69E-46 |
| MS.gene051926 | MS.gene37086 | -0.824517 | 7.73E-54 | -1.69E-46 |
| MS.gene052108 | MS.gene37086 | 0.80995 | 1.47E-50 | -1.69E-46 |
| MS.gene05216 | MS.gene37086 | 0.836247 | 1.05E-56 | -1.69E-46 |
| MS.gene052333 | MS.gene37086 | 0.857851 | 1.23E-62 | -1.69E-46 |
| MS.gene052463 | MS.gene37086 | 0.81002 | 1.42E-50 | -1.69E-46 |
| MS.gene052493 | MS.gene37086 | 0.801785 | 7.64E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene37086 | 0.812123 | 4.97E-51 | -1.69E-46 |
| MS.gene052534 | MS.gene37086 | 0.819992 | 8.68E-53 | -1.69E-46 |
| MS.gene052582 | MS.gene37086 | 0.815613 | 8.45E-52 | -1.69E-46 |
| MS.gene052584 | MS.gene37086 | 0.803261 | 3.79E-49 | -1.69E-46 |
| MS.gene052586 | MS.gene37086 | 0.819696 | 1.01E-52 | -1.69E-46 |
| MS.gene052674 | MS.gene37086 | 0.882606 | 9.05E-71 | -1.69E-46 |
| MS.gene052675 | MS.gene37086 | 0.881703 | 1.93E-70 | -1.69E-46 |
| MS.gene052677 | MS.gene37086 | 0.884528 | 1.78E-71 | -1.69E-46 |
| MS.gene052965 | MS.gene37086 | -0.825371 | 4.86E-54 | -1.69E-46 |
| MS.gene053166 | MS.gene37086 | 0.85599 | 4.35E-62 | -1.69E-46 |
| MS.gene053201 | MS.gene37086 | 0.802023 | 6.83E-49 | -1.69E-46 |
| MS.gene053296 | MS.gene37086 | 0.804536 | 2.06E-49 | -1.69E-46 |
| MS.gene05350 | MS.gene37086 | 0.815872 | 7.40E-52 | -1.69E-46 |
| MS.gene053647 | MS.gene37086 | 0.816113 | 6.54E-52 | -1.69E-46 |
| MS.gene05414 | MS.gene37086 | 0.834406 | 3.05E-56 | -1.69E-46 |
| MS.gene05415 | MS.gene37086 | 0.853961 | 1.69E-61 | -1.69E-46 |
| MS.gene054236 | MS.gene37086 | 0.836464 | 9.22E-57 | -1.69E-46 |
| MS.gene05423 | MS.gene37086 | 0.828934 | 6.82E-55 | -1.69E-46 |
| MS.gene054291 | MS.gene37086 | -0.851072 | 1.13E-60 | -1.69E-46 |
| MS.gene054405 | MS.gene37086 | 0.824166 | 9.35E-54 | -1.69E-46 |
| MS.gene054467 | MS.gene37086 | 0.826898 | 2.11E-54 | -1.69E-46 |
| MS.gene054468 | MS.gene37086 | 0.840357 | 9.13E-58 | -1.69E-46 |
| MS.gene054479 | MS.gene37086 | 0.838278 | 3.16E-57 | -1.69E-46 |
| MS.gene054559 | MS.gene37086 | 0.823929 | 1.06E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene37086 | 0.843851 | 1.09E-58 | -1.69E-46 |
| MS.gene054892 | MS.gene37086 | 0.809629 | 1.72E-50 | -1.69E-46 |
| MS.gene054893 | MS.gene37086 | 0.803798 | 2.94E-49 | -1.69E-46 |
| MS.gene05510 | MS.gene37086 | 0.808702 | 2.72E-50 | -1.69E-46 |
| MS.gene055166 | MS.gene37086 | 0.800185 | 1.62E-48 | -1.69E-46 |
| MS.gene055222 | MS.gene37086 | 0.809909 | 1.50E-50 | -1.69E-46 |
| MS.gene055227 | MS.gene37086 | 0.836315 | 1.01E-56 | -1.69E-46 |
| MS.gene055228 | MS.gene37086 | 0.834063 | 3.72E-56 | -1.69E-46 |
| MS.gene055701 | MS.gene37086 | 0.871523 | 6.41E-67 | -1.69E-46 |
| MS.gene055818 | MS.gene37086 | 0.820189 | 7.82E-53 | -1.69E-46 |
| MS.gene055926 | MS.gene37086 | 0.859639 | 3.60E-63 | -1.69E-46 |
| MS.gene05604 | MS.gene37086 | 0.815124 | 1.09E-51 | -1.69E-46 |
| MS.gene056162 | MS.gene37086 | 0.811148 | 8.09E-51 | -1.69E-46 |
| MS.gene056174 | MS.gene37086 | 0.830113 | 3.53E-55 | -1.69E-46 |
| MS.gene056480 | MS.gene37086 | 0.832045 | 1.18E-55 | -1.69E-46 |
| MS.gene056506 | MS.gene37086 | 0.828418 | 9.09E-55 | -1.69E-46 |
| MS.gene056776 | MS.gene37086 | 0.818821 | 1.60E-52 | -1.69E-46 |
| MS.gene056779 | MS.gene37086 | 0.85022 | 1.95E-60 | -1.69E-46 |
| MS.gene056873 | MS.gene37086 | 0.828276 | 9.84E-55 | -1.69E-46 |
| MS.gene057012 | MS.gene37086 | 0.841391 | 4.89E-58 | -1.69E-46 |
| MS.gene057026 | MS.gene37086 | 0.802099 | 6.59E-49 | -1.69E-46 |
| MS.gene057156 | MS.gene37086 | -0.867422 | 1.38E-65 | -1.69E-46 |
| MS.gene057208 | MS.gene37086 | 0.858195 | 9.73E-63 | -1.69E-46 |
| MS.gene057444 | MS.gene37086 | 0.805664 | 1.20E-49 | -1.69E-46 |
| MS.gene05761 | MS.gene37086 | 0.824828 | 6.53E-54 | -1.69E-46 |
| MS.gene057869 | MS.gene37086 | 0.825467 | 4.61E-54 | -1.69E-46 |
| MS.gene058010 | MS.gene37086 | 0.804712 | 1.89E-49 | -1.69E-46 |
| MS.gene058081 | MS.gene37086 | 0.808041 | 3.76E-50 | -1.69E-46 |
| MS.gene058223 | MS.gene37086 | -0.829036 | 6.44E-55 | -1.69E-46 |
| MS.gene058294 | MS.gene37086 | 0.826972 | 2.02E-54 | -1.69E-46 |
| MS.gene058429 | MS.gene37086 | 0.80307 | 4.15E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene37086 | 0.815048 | 1.13E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene37086 | 0.823181 | 1.59E-53 | -1.69E-46 |
| MS.gene059160 | MS.gene37086 | 0.814266 | 1.68E-51 | -1.69E-46 |
| MS.gene059161 | MS.gene37086 | 0.871048 | 9.19E-67 | -1.69E-46 |
| MS.gene059162 | MS.gene37086 | 0.831191 | 1.92E-55 | -1.69E-46 |
| MS.gene059283 | MS.gene37086 | 0.802408 | 5.69E-49 | -1.69E-46 |
| MS.gene059438 | MS.gene37086 | 0.820057 | 8.39E-53 | -1.69E-46 |
| MS.gene059439 | MS.gene37086 | 0.809891 | 1.51E-50 | -1.69E-46 |
| MS.gene059440 | MS.gene37086 | 0.819627 | 1.05E-52 | -1.69E-46 |
| MS.gene059550 | MS.gene37086 | 0.802262 | 6.10E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene37086 | 0.811146 | 8.10E-51 | -1.69E-46 |
| MS.gene059609 | MS.gene37086 | 0.811194 | 7.91E-51 | -1.69E-46 |
| MS.gene060011 | MS.gene37086 | 0.864297 | 1.35E-64 | -1.69E-46 |
| MS.gene060122 | MS.gene37086 | -0.807763 | 4.31E-50 | -1.69E-46 |
| MS.gene060137 | MS.gene37086 | -0.800545 | 1.37E-48 | -1.69E-46 |
| MS.gene060167 | MS.gene37086 | 0.804055 | 2.60E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene37086 | 0.832997 | 6.87E-56 | -1.69E-46 |
| MS.gene060373 | MS.gene37086 | 0.82612 | 3.23E-54 | -1.69E-46 |
| MS.gene06052 | MS.gene37086 | 0.870037 | 1.98E-66 | -1.69E-46 |
| MS.gene060683 | MS.gene37086 | 0.820497 | 6.65E-53 | -1.69E-46 |
| MS.gene060685 | MS.gene37086 | 0.81971 | 1.01E-52 | -1.69E-46 |
| MS.gene060777 | MS.gene37086 | 0.80574 | 1.15E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene37086 | 0.850176 | 2.01E-60 | -1.69E-46 |
| MS.gene060803 | MS.gene37086 | 0.820269 | 7.50E-53 | -1.69E-46 |
| MS.gene060850 | MS.gene37086 | 0.858237 | 9.45E-63 | -1.69E-46 |
| MS.gene060851 | MS.gene37086 | 0.858621 | 7.26E-63 | -1.69E-46 |
| MS.gene060852 | MS.gene37086 | 0.855916 | 4.58E-62 | -1.69E-46 |
| MS.gene060932 | MS.gene37086 | 0.824763 | 6.77E-54 | -1.69E-46 |
| MS.gene060998 | MS.gene37086 | 0.822703 | 2.06E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37086.t1 | MTR_8g089850 | 96.061 | 330 | 9 | 1 | 1 | 330 | 1 | 326 | 0.0 | 641 |
| MS.gene37086.t1 | MTR_3g086670 | 62.349 | 332 | 116 | 6 | 3 | 330 | 2 | 328 | 2.62e-136 | 390 |
| MS.gene37086.t1 | MTR_3g099670 | 38.014 | 292 | 170 | 5 | 9 | 292 | 10 | 298 | 6.12e-56 | 185 |
| MS.gene37086.t1 | MTR_3g106900 | 31.579 | 152 | 99 | 3 | 1 | 149 | 1 | 150 | 7.30e-16 | 79.0 |
| MS.gene37086.t1 | MTR_4g127440 | 24.710 | 259 | 178 | 6 | 27 | 274 | 30 | 282 | 1.06e-15 | 78.2 |
| MS.gene37086.t1 | MTR_4g115420 | 28.061 | 196 | 124 | 6 | 2 | 190 | 5 | 190 | 1.66e-13 | 71.6 |
| MS.gene37086.t1 | MTR_2g037980 | 26.357 | 258 | 174 | 7 | 27 | 274 | 30 | 281 | 2.60e-13 | 70.9 |
| MS.gene37086.t1 | MTR_8g008860 | 30.065 | 153 | 101 | 3 | 1 | 149 | 1 | 151 | 3.77e-13 | 70.5 |
| MS.gene37086.t1 | MTR_2g008170 | 29.870 | 154 | 100 | 4 | 1 | 149 | 1 | 151 | 8.15e-13 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37086.t1 | AT4G40080 | 49.003 | 351 | 153 | 9 | 1 | 328 | 1 | 348 | 2.14e-98 | 295 |
| MS.gene37086.t1 | AT5G10410 | 34.058 | 276 | 154 | 10 | 7 | 265 | 6 | 270 | 6.31e-32 | 122 |
| MS.gene37086.t1 | AT5G65370 | 29.181 | 281 | 168 | 9 | 1 | 262 | 1 | 269 | 8.93e-24 | 99.4 |
| MS.gene37086.t1 | AT5G57200 | 24.125 | 257 | 180 | 6 | 27 | 274 | 30 | 280 | 5.22e-14 | 73.2 |
| MS.gene37086.t1 | AT4G32285 | 28.859 | 149 | 101 | 3 | 4 | 149 | 3 | 149 | 1.09e-13 | 72.4 |
| MS.gene37086.t1 | AT4G32285 | 28.859 | 149 | 101 | 3 | 4 | 149 | 3 | 149 | 1.09e-13 | 72.4 |
| MS.gene37086.t1 | AT2G25430 | 29.730 | 148 | 99 | 3 | 5 | 149 | 4 | 149 | 1.41e-13 | 72.0 |
| MS.gene37086.t1 | AT4G02650 | 30.345 | 145 | 94 | 3 | 10 | 149 | 10 | 152 | 3.21e-13 | 70.9 |
| MS.gene37086.t1 | AT4G25940 | 24.561 | 285 | 188 | 7 | 10 | 274 | 11 | 288 | 3.51e-13 | 70.9 |
| MS.gene37086.t1 | AT2G01600 | 33.333 | 129 | 81 | 4 | 31 | 157 | 34 | 159 | 5.00e-13 | 70.1 |
| MS.gene37086.t1 | AT1G03050 | 30.345 | 145 | 94 | 3 | 10 | 149 | 10 | 152 | 3.34e-12 | 67.8 |
| MS.gene37086.t1 | AT1G14910 | 30.233 | 129 | 85 | 4 | 31 | 157 | 34 | 159 | 4.98e-11 | 64.3 |
Find 100 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTGAAGTGAATGAGTTTA+AGG | 0.146193 | 4.3:+10558874 | MS.gene37086:CDS |
| TGTCTTTGATTATGTTTATT+AGG | 0.192374 | 4.3:-10558211 | None:intergenic |
| CTTTGTACTTCACGAATATT+AGG | 0.195912 | 4.3:+10558632 | MS.gene37086:CDS |
| AGAGAGGGTGGCGATGATTT+TGG | 0.211056 | 4.3:+10558970 | MS.gene37086:CDS |
| AGGTTAGGGTGTTTGGAATT+TGG | 0.217369 | 4.3:+10558899 | MS.gene37086:CDS |
| ATATTAGGTTTCTTCTTTCT+TGG | 0.217904 | 4.3:+10558647 | MS.gene37086:CDS |
| CATGCGTGGTGGCGCGTAAA+AGG | 0.245276 | 4.3:-10558283 | None:intergenic |
| TAAGGAGAGGTTAGGGTGTT+TGG | 0.259878 | 4.3:+10558892 | MS.gene37086:CDS |
| GTTGAAGGAGTTTGATTCTT+TGG | 0.272817 | 4.3:+10558724 | MS.gene37086:CDS |
| TTGTTCATGTTCACTTGATC+TGG | 0.299217 | 4.3:-10558776 | None:intergenic |
| GGTGGATGAGATAGTGAATT+TGG | 0.313395 | 4.3:+10558814 | MS.gene37086:CDS |
| AATGAGTTTAAGGAGAGGTT+AGG | 0.335488 | 4.3:+10558884 | MS.gene37086:CDS |
| GGTTGGAATGGAGGTGTATA+AGG | 0.337777 | 4.3:+10559039 | MS.gene37086:CDS |
| TGAATTTGGTTTGTGAAGAT+TGG | 0.373475 | 4.3:+10558828 | MS.gene37086:CDS |
| TTCGTGGGAACTTTCTTGTT+GGG | 0.385009 | 4.3:+10558577 | MS.gene37086:CDS |
| GAGAGCAACGGCGGCGTTGT+TGG | 0.403727 | 4.3:-10558420 | None:intergenic |
| CTTCGTGGGAACTTTCTTGT+TGG | 0.404521 | 4.3:+10558576 | MS.gene37086:CDS |
| GGAAAAGGGTAGGGTGCATA+AGG | 0.410677 | 4.3:+10559060 | MS.gene37086:CDS |
| TTCTTTGGTGACACTGGTTG+AGG | 0.421524 | 4.3:+10558739 | MS.gene37086:CDS |
| CCCTTTGGTTTCCCTCTGGA+AGG | 0.426989 | 4.3:+10559149 | MS.gene37086:CDS |
| TCAGTTCTCGTGATTCCCTT+TGG | 0.432756 | 4.3:+10559134 | MS.gene37086:CDS |
| ATTTGGAGAAGCTGTTGAAT+TGG | 0.433804 | 4.3:+10558916 | MS.gene37086:CDS |
| GAATTTGGTTTGTGAAGATT+GGG | 0.435014 | 4.3:+10558829 | MS.gene37086:CDS |
| AACTTTCTTGTTGGGTACGC+TGG | 0.446346 | 4.3:+10558585 | MS.gene37086:CDS |
| TACAAACTGTGGTTTGTTGA+AGG | 0.449445 | 4.3:+10558709 | MS.gene37086:CDS |
| ACAGCAAGGGTTGTGGGATT+CGG | 0.450595 | 4.3:+10558997 | MS.gene37086:CDS |
| GGAGTCACAGCAAGGGTTGT+GGG | 0.451429 | 4.3:+10558991 | MS.gene37086:CDS |
| TTATCTGTTTACCCTTCAAC+CGG | 0.456568 | 4.3:+10558503 | MS.gene37086:CDS |
| AATGGAGGTGTATAAGGAAA+AGG | 0.456653 | 4.3:+10559045 | MS.gene37086:CDS |
| GGTGAGAGAGGTGAAGGATA+AGG | 0.466786 | 4.3:+10559018 | MS.gene37086:CDS |
| TGCGCGTGAACCGTCGGTGG+AGG | 0.467786 | 4.3:-10558351 | None:intergenic |
| AAAGGGTAGGGTGCATAAGG+AGG | 0.471808 | 4.3:+10559063 | MS.gene37086:CDS |
| CAAGAAAGTTCCCACGAAGT+TGG | 0.471951 | 4.3:-10558572 | None:intergenic |
| GATTCCCTTTGGTTTCCCTC+TGG | 0.472352 | 4.3:+10559145 | MS.gene37086:CDS |
| ATAAGGAGGGAAGAAAGTTA+AGG | 0.477466 | 4.3:+10559077 | MS.gene37086:CDS |
| ATGAGTTTAAGGAGAGGTTA+GGG | 0.479103 | 4.3:+10558885 | MS.gene37086:CDS |
| AGAGAGGTGAAGGATAAGGT+TGG | 0.486121 | 4.3:+10559022 | MS.gene37086:CDS |
| CTCCTTCTTAGAACTCCTCA+TGG | 0.486237 | 4.3:+10558382 | MS.gene37086:CDS |
| TGGAGTCACAGCAAGGGTTG+TGG | 0.486285 | 4.3:+10558990 | MS.gene37086:CDS |
| ATGCGTGGTGGCGCGTAAAA+GGG | 0.490324 | 4.3:-10558282 | None:intergenic |
| AAGGGTAGGGTGCATAAGGA+GGG | 0.491561 | 4.3:+10559064 | MS.gene37086:CDS |
| TCTTTGGTGACACTGGTTGA+GGG | 0.496012 | 4.3:+10558740 | MS.gene37086:CDS |
| GGTTTGTTGTTTGAAGAGGT+TGG | 0.498420 | 4.3:+10558937 | MS.gene37086:CDS |
| GCGTAAAAGGGAGAGGGTGG+TGG | 0.501291 | 4.3:-10558270 | None:intergenic |
| TAGTTTCTACCACCGGTTGA+AGG | 0.501899 | 4.3:-10558515 | None:intergenic |
| ATGGAGGTGTATAAGGAAAA+GGG | 0.504494 | 4.3:+10559046 | MS.gene37086:CDS |
| CTTTGGTGACACTGGTTGAG+GGG | 0.505079 | 4.3:+10558741 | MS.gene37086:CDS |
| GGTGAAGGATAAGGTTGGAA+TGG | 0.506608 | 4.3:+10559027 | MS.gene37086:CDS |
| CGTGGAGATATGTTTGTGGG+TGG | 0.510992 | 4.3:-10558321 | None:intergenic |
| TTTGTGGGTGGGAGGAGTGA+AGG | 0.525799 | 4.3:-10558309 | None:intergenic |
| AATTGGTTTGTTGTTTGAAG+AGG | 0.527047 | 4.3:+10558933 | MS.gene37086:CDS |
| GAGTTCTAAGAAGGAGGAAG+CGG | 0.537195 | 4.3:-10558375 | None:intergenic |
| GTTAAGATAGTTTCTACCAC+CGG | 0.540411 | 4.3:-10558522 | None:intergenic |
| GTTTGATTCTTTGGTGACAC+TGG | 0.541118 | 4.3:+10558733 | MS.gene37086:CDS |
| ACGCTCCTCTCCTCCACCGA+CGG | 0.543777 | 4.3:+10558341 | MS.gene37086:CDS |
| GAAGGATAAGGTTGGAATGG+AGG | 0.543884 | 4.3:+10559030 | MS.gene37086:CDS |
| TGGTGGCGCGTAAAAGGGAG+AGG | 0.552428 | 4.3:-10558277 | None:intergenic |
| GTCCATGAGGAGTTCTAAGA+AGG | 0.553775 | 4.3:-10558384 | None:intergenic |
| GGATTCGGTGAGAGAGGTGA+AGG | 0.555628 | 4.3:+10559012 | MS.gene37086:CDS |
| GGTTGGAGGAGTGTAAAGAG+AGG | 0.557650 | 4.3:+10558954 | MS.gene37086:CDS |
| CTTGAAGTATGAAGCTTCCA+TGG | 0.558623 | 4.3:-10558475 | None:intergenic |
| CTTGGAAAATCTGTGTGTGA+TGG | 0.558786 | 4.3:+10558665 | MS.gene37086:CDS |
| GTGGAGATATGTTTGTGGGT+GGG | 0.558835 | 4.3:-10558320 | None:intergenic |
| GGAGCGTGGAGATATGTTTG+TGG | 0.559172 | 4.3:-10558325 | None:intergenic |
| AGTTTCTACCACCGGTTGAA+GGG | 0.559859 | 4.3:-10558514 | None:intergenic |
| GTTGTGGGATTCGGTGAGAG+AGG | 0.560768 | 4.3:+10559006 | MS.gene37086:CDS |
| AGCGGTTGCGCGTGAACCGT+CGG | 0.562609 | 4.3:-10558357 | None:intergenic |
| GGAGGAGTGTAAAGAGAGGG+TGG | 0.564100 | 4.3:+10558958 | MS.gene37086:CDS |
| TAAGGTTTAGTGAGTCTGAT+CGG | 0.565323 | 4.3:+10559095 | MS.gene37086:CDS |
| GTTGGAGGAGTGTAAAGAGA+GGG | 0.565591 | 4.3:+10558955 | MS.gene37086:CDS |
| GAGCGTGGAGATATGTTTGT+GGG | 0.568041 | 4.3:-10558324 | None:intergenic |
| ATAATACAAATCCAACTTCG+TGG | 0.571622 | 4.3:+10558561 | MS.gene37086:CDS |
| CCTTCCAGAGGGAAACCAAA+GGG | 0.572238 | 4.3:-10559149 | None:intergenic |
| TTGTTGTTTGAAGAGGTTGG+AGG | 0.572648 | 4.3:+10558940 | MS.gene37086:CDS |
| GAGATATGTTTGTGGGTGGG+AGG | 0.573647 | 4.3:-10558317 | None:intergenic |
| TAATACAAATCCAACTTCGT+GGG | 0.573656 | 4.3:+10558562 | MS.gene37086:CDS |
| CAAGAACAAGAACAAGTTGG+TGG | 0.583931 | 4.3:+10558796 | MS.gene37086:CDS |
| GAACAAGAACAAGAACAAGT+TGG | 0.588041 | 4.3:+10558793 | MS.gene37086:CDS |
| GGCGCGTAAAAGGGAGAGGG+TGG | 0.589270 | 4.3:-10558273 | None:intergenic |
| GGTGTATAAGGAAAAGGGTA+GGG | 0.591904 | 4.3:+10559051 | MS.gene37086:CDS |
| AGGTGTATAAGGAAAAGGGT+AGG | 0.594328 | 4.3:+10559050 | MS.gene37086:CDS |
| GTTCATCATATCATCAGCCA+TGG | 0.595539 | 4.3:+10558458 | MS.gene37086:CDS |
| GTCGGTGGAGGAGAGGAGCG+TGG | 0.605829 | 4.3:-10558339 | None:intergenic |
| CATGAGGAGTTCTAAGAAGG+AGG | 0.607142 | 4.3:-10558381 | None:intergenic |
| GTGAACCGTCGGTGGAGGAG+AGG | 0.607639 | 4.3:-10558346 | None:intergenic |
| TTTGGTTTGTGAAGATTGGG+TGG | 0.619906 | 4.3:+10558832 | MS.gene37086:CDS |
| AAGTGAATGAGTTTAAGGAG+AGG | 0.627174 | 4.3:+10558879 | MS.gene37086:CDS |
| GTTTCTTGTCTTACAAACTG+TGG | 0.632756 | 4.3:+10558698 | MS.gene37086:CDS |
| CTTTACAATGACCTTCCAGA+GGG | 0.633407 | 4.3:-10559160 | None:intergenic |
| ACCTTCCAGAGGGAAACCAA+AGG | 0.638530 | 4.3:-10559150 | None:intergenic |
| TATTATGTCGAAAATTCGAG+AGG | 0.648120 | 4.3:-10558544 | None:intergenic |
| GAGAGACTTGAGAGCAACGG+CGG | 0.649205 | 4.3:-10558429 | None:intergenic |
| TCTGTTTACCCTTCAACCGG+TGG | 0.651507 | 4.3:+10558506 | MS.gene37086:CDS |
| GGTGGCGCGTAAAAGGGAGA+GGG | 0.653956 | 4.3:-10558276 | None:intergenic |
| GGTTGCGCGTGAACCGTCGG+TGG | 0.664259 | 4.3:-10558354 | None:intergenic |
| AGTGAAGGAGTCATGCGTGG+TGG | 0.667894 | 4.3:-10558294 | None:intergenic |
| TTGGGTGGTGATAGAAACTG+TGG | 0.673066 | 4.3:+10558847 | MS.gene37086:CDS |
| GATGAGAGACTTGAGAGCAA+CGG | 0.674885 | 4.3:-10558432 | None:intergenic |
| AGGAGTGAAGGAGTCATGCG+TGG | 0.688189 | 4.3:-10558297 | None:intergenic |
| ACTTTACAATGACCTTCCAG+AGG | 0.693234 | 4.3:-10559161 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTCTTTGATTATGTTTATT+AGG | - | chr4.3:10558214-10558233 | None:intergenic | 20.0% |
| ! | ATATTAGGTTTCTTCTTTCT+TGG | + | chr4.3:10558647-10558666 | MS.gene37086:CDS | 25.0% |
| AGTTGAAGTGAATGAGTTTA+AGG | + | chr4.3:10558874-10558893 | MS.gene37086:CDS | 30.0% | |
| ATAATACAAATCCAACTTCG+TGG | + | chr4.3:10558561-10558580 | MS.gene37086:CDS | 30.0% | |
| CTTTGTACTTCACGAATATT+AGG | + | chr4.3:10558632-10558651 | MS.gene37086:CDS | 30.0% | |
| TAATACAAATCCAACTTCGT+GGG | + | chr4.3:10558562-10558581 | MS.gene37086:CDS | 30.0% | |
| TATTATGTCGAAAATTCGAG+AGG | - | chr4.3:10558547-10558566 | None:intergenic | 30.0% | |
| !! | AATTGGTTTGTTGTTTGAAG+AGG | + | chr4.3:10558933-10558952 | MS.gene37086:CDS | 30.0% |
| !! | GAATTTGGTTTGTGAAGATT+GGG | + | chr4.3:10558829-10558848 | MS.gene37086:CDS | 30.0% |
| !! | TGAATTTGGTTTGTGAAGAT+TGG | + | chr4.3:10558828-10558847 | MS.gene37086:CDS | 30.0% |
| AAGTGAATGAGTTTAAGGAG+AGG | + | chr4.3:10558879-10558898 | MS.gene37086:CDS | 35.0% | |
| AATGAGTTTAAGGAGAGGTT+AGG | + | chr4.3:10558884-10558903 | MS.gene37086:CDS | 35.0% | |
| ATAAGGAGGGAAGAAAGTTA+AGG | + | chr4.3:10559077-10559096 | MS.gene37086:CDS | 35.0% | |
| ATGAGTTTAAGGAGAGGTTA+GGG | + | chr4.3:10558885-10558904 | MS.gene37086:CDS | 35.0% | |
| ATTTGGAGAAGCTGTTGAAT+TGG | + | chr4.3:10558916-10558935 | MS.gene37086:CDS | 35.0% | |
| GAACAAGAACAAGAACAAGT+TGG | + | chr4.3:10558793-10558812 | MS.gene37086:CDS | 35.0% | |
| GTTAAGATAGTTTCTACCAC+CGG | - | chr4.3:10558525-10558544 | None:intergenic | 35.0% | |
| GTTGAAGGAGTTTGATTCTT+TGG | + | chr4.3:10558724-10558743 | MS.gene37086:CDS | 35.0% | |
| GTTTCTTGTCTTACAAACTG+TGG | + | chr4.3:10558698-10558717 | MS.gene37086:CDS | 35.0% | |
| TAAGGTTTAGTGAGTCTGAT+CGG | + | chr4.3:10559095-10559114 | MS.gene37086:CDS | 35.0% | |
| TTATCTGTTTACCCTTCAAC+CGG | + | chr4.3:10558503-10558522 | MS.gene37086:CDS | 35.0% | |
| TTGTTCATGTTCACTTGATC+TGG | - | chr4.3:10558779-10558798 | None:intergenic | 35.0% | |
| ! | AATGGAGGTGTATAAGGAAA+AGG | + | chr4.3:10559045-10559064 | MS.gene37086:CDS | 35.0% |
| ! | ATGGAGGTGTATAAGGAAAA+GGG | + | chr4.3:10559046-10559065 | MS.gene37086:CDS | 35.0% |
| !! | TACAAACTGTGGTTTGTTGA+AGG | + | chr4.3:10558709-10558728 | MS.gene37086:CDS | 35.0% |
| AGGTGTATAAGGAAAAGGGT+AGG | + | chr4.3:10559050-10559069 | MS.gene37086:CDS | 40.0% | |
| CTTGAAGTATGAAGCTTCCA+TGG | - | chr4.3:10558478-10558497 | None:intergenic | 40.0% | |
| CTTGGAAAATCTGTGTGTGA+TGG | + | chr4.3:10558665-10558684 | MS.gene37086:CDS | 40.0% | |
| GGTGGATGAGATAGTGAATT+TGG | + | chr4.3:10558814-10558833 | MS.gene37086:CDS | 40.0% | |
| GGTGTATAAGGAAAAGGGTA+GGG | + | chr4.3:10559051-10559070 | MS.gene37086:CDS | 40.0% | |
| GTTCATCATATCATCAGCCA+TGG | + | chr4.3:10558458-10558477 | MS.gene37086:CDS | 40.0% | |
| TTCGTGGGAACTTTCTTGTT+GGG | + | chr4.3:10558577-10558596 | MS.gene37086:CDS | 40.0% | |
| ! | AGGTTAGGGTGTTTGGAATT+TGG | + | chr4.3:10558899-10558918 | MS.gene37086:CDS | 40.0% |
| ! | CAAGAACAAGAACAAGTTGG+TGG | + | chr4.3:10558796-10558815 | MS.gene37086:CDS | 40.0% |
| ! | GTTTGATTCTTTGGTGACAC+TGG | + | chr4.3:10558733-10558752 | MS.gene37086:CDS | 40.0% |
| !! | GGTTTGTTGTTTGAAGAGGT+TGG | + | chr4.3:10558937-10558956 | MS.gene37086:CDS | 40.0% |
| !! | TTGTTGTTTGAAGAGGTTGG+AGG | + | chr4.3:10558940-10558959 | MS.gene37086:CDS | 40.0% |
| !! | TTTGGTTTGTGAAGATTGGG+TGG | + | chr4.3:10558832-10558851 | MS.gene37086:CDS | 40.0% |
| !!! | ATGATTTTGGAGTCACAGCA+AGG | + | chr4.3:10558983-10559002 | MS.gene37086:CDS | 40.0% |
| !!! | CGTTGTTGGTGTTTTGAAGA+CGG | - | chr4.3:10558409-10558428 | None:intergenic | 40.0% |
| !!! | TGATTTTGGAGTCACAGCAA+GGG | + | chr4.3:10558984-10559003 | MS.gene37086:CDS | 40.0% |
| AACTTTCTTGTTGGGTACGC+TGG | + | chr4.3:10558585-10558604 | MS.gene37086:CDS | 45.0% | |
| AGAGAGGTGAAGGATAAGGT+TGG | + | chr4.3:10559022-10559041 | MS.gene37086:CDS | 45.0% | |
| CAAGAAAGTTCCCACGAAGT+TGG | - | chr4.3:10558575-10558594 | None:intergenic | 45.0% | |
| CTCCTTCTTAGAACTCCTCA+TGG | + | chr4.3:10558382-10558401 | MS.gene37086:CDS | 45.0% | |
| CTTCGTGGGAACTTTCTTGT+TGG | + | chr4.3:10558576-10558595 | MS.gene37086:CDS | 45.0% | |
| GAAGGATAAGGTTGGAATGG+AGG | + | chr4.3:10559030-10559049 | MS.gene37086:CDS | 45.0% | |
| GAGCGTGGAGATATGTTTGT+GGG | - | chr4.3:10558327-10558346 | None:intergenic | 45.0% | |
| GATGAGAGACTTGAGAGCAA+CGG | - | chr4.3:10558435-10558454 | None:intergenic | 45.0% | |
| GGTGAAGGATAAGGTTGGAA+TGG | + | chr4.3:10559027-10559046 | MS.gene37086:CDS | 45.0% | |
| GTGGAGATATGTTTGTGGGT+GGG | - | chr4.3:10558323-10558342 | None:intergenic | 45.0% | |
| TCAGTTCTCGTGATTCCCTT+TGG | + | chr4.3:10559134-10559153 | MS.gene37086:CDS | 45.0% | |
| TTGGGTGGTGATAGAAACTG+TGG | + | chr4.3:10558847-10558866 | MS.gene37086:CDS | 45.0% | |
| ! | AGTTTCTACCACCGGTTGAA+GGG | - | chr4.3:10558517-10558536 | None:intergenic | 45.0% |
| ! | GGTTGGAATGGAGGTGTATA+AGG | + | chr4.3:10559039-10559058 | MS.gene37086:CDS | 45.0% |
| ! | GTTGGAGGAGTGTAAAGAGA+GGG | + | chr4.3:10558955-10558974 | MS.gene37086:CDS | 45.0% |
| ! | TAAGGAGAGGTTAGGGTGTT+TGG | + | chr4.3:10558892-10558911 | MS.gene37086:CDS | 45.0% |
| ! | TAGTTTCTACCACCGGTTGA+AGG | - | chr4.3:10558518-10558537 | None:intergenic | 45.0% |
| ! | TCTTTGGTGACACTGGTTGA+GGG | + | chr4.3:10558740-10558759 | MS.gene37086:CDS | 45.0% |
| ! | TTCTTTGGTGACACTGGTTG+AGG | + | chr4.3:10558739-10558758 | MS.gene37086:CDS | 45.0% |
| !! | CATGAGGAGTTCTAAGAAGG+AGG | - | chr4.3:10558384-10558403 | None:intergenic | 45.0% |
| !! | GAGTTCTAAGAAGGAGGAAG+CGG | - | chr4.3:10558378-10558397 | None:intergenic | 45.0% |
| !! | GTCCATGAGGAGTTCTAAGA+AGG | - | chr4.3:10558387-10558406 | None:intergenic | 45.0% |
| !!! | TGTTTTGAAGACGGTCCATG+AGG | - | chr4.3:10558400-10558419 | None:intergenic | 45.0% |
| AAAGGGTAGGGTGCATAAGG+AGG | + | chr4.3:10559063-10559082 | MS.gene37086:CDS | 50.0% | |
| AAGGGTAGGGTGCATAAGGA+GGG | + | chr4.3:10559064-10559083 | MS.gene37086:CDS | 50.0% | |
| ACAGCAAGGGTTGTGGGATT+CGG | + | chr4.3:10558997-10559016 | MS.gene37086:CDS | 50.0% | |
| ACCTTCCAGAGGGAAACCAA+AGG | - | chr4.3:10559153-10559172 | None:intergenic | 50.0% | |
| CCTTCCAGAGGGAAACCAAA+GGG | - | chr4.3:10559152-10559171 | None:intergenic | 50.0% | |
| CGTGGAGATATGTTTGTGGG+TGG | - | chr4.3:10558324-10558343 | None:intergenic | 50.0% | |
| GAGATATGTTTGTGGGTGGG+AGG | - | chr4.3:10558320-10558339 | None:intergenic | 50.0% | |
| GGAAAAGGGTAGGGTGCATA+AGG | + | chr4.3:10559060-10559079 | MS.gene37086:CDS | 50.0% | |
| GGAGCGTGGAGATATGTTTG+TGG | - | chr4.3:10558328-10558347 | None:intergenic | 50.0% | |
| GGTGAGAGAGGTGAAGGATA+AGG | + | chr4.3:10559018-10559037 | MS.gene37086:CDS | 50.0% | |
| TCTGTTTACCCTTCAACCGG+TGG | + | chr4.3:10558506-10558525 | MS.gene37086:CDS | 50.0% | |
| ! | AGAGAGGGTGGCGATGATTT+TGG | + | chr4.3:10558970-10558989 | MS.gene37086:CDS | 50.0% |
| ! | CTTTGGTGACACTGGTTGAG+GGG | + | chr4.3:10558741-10558760 | MS.gene37086:CDS | 50.0% |
| ! | GATTCCCTTTGGTTTCCCTC+TGG | + | chr4.3:10559145-10559164 | MS.gene37086:CDS | 50.0% |
| ! | GGTTGGAGGAGTGTAAAGAG+AGG | + | chr4.3:10558954-10558973 | MS.gene37086:CDS | 50.0% |
| AGGAGTGAAGGAGTCATGCG+TGG | - | chr4.3:10558300-10558319 | None:intergenic | 55.0% | |
| AGTGAAGGAGTCATGCGTGG+TGG | - | chr4.3:10558297-10558316 | None:intergenic | 55.0% | |
| GAGAGACTTGAGAGCAACGG+CGG | - | chr4.3:10558432-10558451 | None:intergenic | 55.0% | |
| GGAGTCACAGCAAGGGTTGT+GGG | + | chr4.3:10558991-10559010 | MS.gene37086:CDS | 55.0% | |
| TGGAGTCACAGCAAGGGTTG+TGG | + | chr4.3:10558990-10559009 | MS.gene37086:CDS | 55.0% | |
| TTTGTGGGTGGGAGGAGTGA+AGG | - | chr4.3:10558312-10558331 | None:intergenic | 55.0% | |
| ! | ATGCGTGGTGGCGCGTAAAA+GGG | - | chr4.3:10558285-10558304 | None:intergenic | 55.0% |
| ! | CCCTTTGGTTTCCCTCTGGA+AGG | + | chr4.3:10559149-10559168 | MS.gene37086:CDS | 55.0% |
| ! | GGAGGAGTGTAAAGAGAGGG+TGG | + | chr4.3:10558958-10558977 | MS.gene37086:CDS | 55.0% |
| !! | GGATTCGGTGAGAGAGGTGA+AGG | + | chr4.3:10559012-10559031 | MS.gene37086:CDS | 55.0% |
| !! | GTTGTGGGATTCGGTGAGAG+AGG | + | chr4.3:10559006-10559025 | MS.gene37086:CDS | 55.0% |
| GCGTAAAAGGGAGAGGGTGG+TGG | - | chr4.3:10558273-10558292 | None:intergenic | 60.0% | |
| GGTGGCGCGTAAAAGGGAGA+GGG | - | chr4.3:10558279-10558298 | None:intergenic | 60.0% | |
| ! | CATGCGTGGTGGCGCGTAAA+AGG | - | chr4.3:10558286-10558305 | None:intergenic | 60.0% |
| ! | TGGTGGCGCGTAAAAGGGAG+AGG | - | chr4.3:10558280-10558299 | None:intergenic | 60.0% |
| ACGCTCCTCTCCTCCACCGA+CGG | + | chr4.3:10558341-10558360 | MS.gene37086:CDS | 65.0% | |
| AGCGGTTGCGCGTGAACCGT+CGG | - | chr4.3:10558360-10558379 | None:intergenic | 65.0% | |
| GAGAGCAACGGCGGCGTTGT+TGG | - | chr4.3:10558423-10558442 | None:intergenic | 65.0% | |
| GGCGCGTAAAAGGGAGAGGG+TGG | - | chr4.3:10558276-10558295 | None:intergenic | 65.0% | |
| GTGAACCGTCGGTGGAGGAG+AGG | - | chr4.3:10558349-10558368 | None:intergenic | 65.0% | |
| GGTTGCGCGTGAACCGTCGG+TGG | - | chr4.3:10558357-10558376 | None:intergenic | 70.0% | |
| GTCGGTGGAGGAGAGGAGCG+TGG | - | chr4.3:10558342-10558361 | None:intergenic | 70.0% | |
| TGCGCGTGAACCGTCGGTGG+AGG | - | chr4.3:10558354-10558373 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 10558188 | 10559180 | 10558188 | ID=MS.gene37086 |
| chr4.3 | mRNA | 10558188 | 10559180 | 10558188 | ID=MS.gene37086.t1;Parent=MS.gene37086 |
| chr4.3 | exon | 10558188 | 10559180 | 10558188 | ID=MS.gene37086.t1.exon1;Parent=MS.gene37086.t1 |
| chr4.3 | CDS | 10558188 | 10559180 | 10558188 | ID=cds.MS.gene37086.t1;Parent=MS.gene37086.t1 |
| Gene Sequence |
| Protein sequence |
