AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene074393


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074393.t1 MTR_3g078590 100.000 90 0 0 1 90 1 90 2.56e-63 186
MS.gene074393.t1 MTR_3g085890 69.318 88 27 0 1 88 1 88 1.66e-43 136
MS.gene074393.t1 MTR_8g080060 69.318 88 27 0 1 88 1 88 4.04e-43 135
MS.gene074393.t1 MTR_7g016060 51.220 82 40 0 5 86 3 84 3.47e-29 100
MS.gene074393.t1 MTR_3g085890 63.934 61 22 0 27 87 1 61 1.80e-25 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074393.t1 AT3G48140 74.118 85 22 0 4 88 3 87 1.26e-44 139
MS.gene074393.t1 AT3G29970 51.220 82 40 0 5 86 3 84 8.05e-30 101
MS.gene074393.t1 AT3G29970 47.059 68 36 0 18 85 1 68 2.24e-20 77.4
MS.gene074393.t1 AT3G29970 47.059 68 36 0 18 85 1 68 2.24e-20 77.4

Find 28 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATCAGTACCTGACTTC+AGG 0.347160 3.4:+75117125 None:intergenic
TACCCTCTGTTTGCTGCGGT+TGG 0.396092 3.4:-75117200 MS.gene074393:CDS
CTGCGGTTGGAGCTGCTGTT+GGG 0.406881 3.4:-75117187 MS.gene074393:CDS
GCTGCGGTTGGAGCTGCTGT+TGG 0.426937 3.4:-75117188 MS.gene074393:CDS
TTGAGGCCGGGCATAATCTC+TGG 0.440854 3.4:+75116071 None:intergenic
AACATCAGTACCTGACTTCA+GGG 0.462667 3.4:+75117126 None:intergenic
AACAAGCAGTCTCGAGCTGC+AGG 0.464020 3.4:-75116167 MS.gene074393:CDS
AGTGAAGAAGCTGTTGAGGC+CGG 0.469984 3.4:+75116058 None:intergenic
GTCTCGAGCTGCAGGAGTGT+TGG 0.483795 3.4:-75116159 MS.gene074393:CDS
GGGTCTGATCCAGCGATTGG+TGG 0.513757 3.4:+75117314 None:intergenic
AAATAACTTAGTTGCGACTT+GGG 0.516597 3.4:+75116033 None:intergenic
TTCTGTGTACTTCTCTCCCT+CGG 0.519035 3.4:+75116127 None:intergenic
CTCGGGTCTGATCCAGCGAT+TGG 0.519677 3.4:+75117311 None:intergenic
GTGAAGAAGCTGTTGAGGCC+GGG 0.521542 3.4:+75116059 None:intergenic
TATCCATGGCCACCAATCGC+TGG 0.522115 3.4:-75117323 None:intergenic
TCTGCATCAACCCTGAAGTC+AGG 0.524860 3.4:-75117136 MS.gene074393:intron
AAAATAACTTAGTTGCGACT+TGG 0.526355 3.4:+75116032 None:intergenic
GATGTTACGAACAAGTGAAA+AGG 0.535731 3.4:+75117157 None:intergenic
AGATCACCAGAGATTATGCC+CGG 0.537901 3.4:-75116077 MS.gene074393:CDS
GATCCAGCGATTGGTGGCCA+TGG 0.584846 3.4:+75117320 None:intergenic
CTCCAACCGCAGCAAACAGA+GGG 0.612181 3.4:+75117198 None:intergenic
GTGTTGGAGAACTTTGCCGA+GGG 0.614956 3.4:-75116143 MS.gene074393:CDS
GGTATACCCTCTGTTTGCTG+CGG 0.632424 3.4:-75117204 MS.gene074393:CDS
AGTGTTGGAGAACTTTGCCG+AGG 0.642190 3.4:-75116144 MS.gene074393:CDS
GGTCAGTGAAGAAGCTGTTG+AGG 0.648101 3.4:+75116054 None:intergenic
GCTCCAACCGCAGCAAACAG+AGG 0.651727 3.4:+75117197 None:intergenic
CAATCGCTGGATCAGACCCG+AGG 0.698764 3.4:-75117310 MS.gene074393:intron
AGTACACAGAACATTTGCTG+AGG 0.706764 3.4:-75116115 MS.gene074393:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATATTAACAAAATTTAAT+GGG - chr3.4:75116472-75116491 MS.gene074393:intron 10.0%
!! TAGATATTAACAAAATTTAA+TGG - chr3.4:75116471-75116490 MS.gene074393:intron 10.0%
!! ATTAATCTCTAATATCAAAT+AGG + chr3.4:75116719-75116738 None:intergenic 15.0%
!! TTAATCTCTAATATCAAATA+GGG + chr3.4:75116718-75116737 None:intergenic 15.0%
!!! AGAGATTAATTTTTATTCAT+TGG - chr3.4:75116729-75116748 MS.gene074393:intron 15.0%
!!! GAATATTCCTTTTTTATTTT+TGG - chr3.4:75116392-75116411 MS.gene074393:intron 15.0%
!! AATTAAATAACTCATGTATC+TGG - chr3.4:75116549-75116568 MS.gene074393:intron 20.0%
!! ACAAATTGTGAAAATTTAAG+CGG + chr3.4:75116448-75116467 None:intergenic 20.0%
!! AGGAAAACCAAAAATAAAAA+AGG + chr3.4:75116402-75116421 None:intergenic 20.0%
!! CTAATCGGATAATTAATTAA+TGG + chr3.4:75116361-75116380 None:intergenic 20.0%
!! GATACATGAGTTATTTAATT+TGG + chr3.4:75116549-75116568 None:intergenic 20.0%
!! TATACCTATAACAAAAAAGA+TGG + chr3.4:75116140-75116159 None:intergenic 20.0%
!!! GATTAATTTTTATTCATTGG+AGG - chr3.4:75116732-75116751 MS.gene074393:intron 20.0%
!!! TGATTAACTAAGTGATTTTT+AGG - chr3.4:75116584-75116603 MS.gene074393:intron 20.0%
! ATTCCATCATGTTTACTAAT+CGG + chr3.4:75116376-75116395 None:intergenic 25.0%
! TGAGATATAACTTAAAAGAG+TGG - chr3.4:75116886-75116905 MS.gene074393:intron 25.0%
! TTTGCACAAATAACGTTTAA+TGG - chr3.4:75116671-75116690 MS.gene074393:intron 25.0%
!! AATGAAAACTTTTATCTCAC+AGG - chr3.4:75117076-75117095 MS.gene074393:intron 25.0%
!! ATGAAAACTTTTATCTCACA+GGG - chr3.4:75117077-75117096 MS.gene074393:intron 25.0%
!! ATGTATGTTTGTTCTTTTGA+TGG - chr3.4:75117158-75117177 MS.gene074393:CDS 25.0%
!! TGTATGTTTGTTCTTTTGAT+GGG - chr3.4:75117159-75117178 MS.gene074393:CDS 25.0%
!!! AATGGTGATTTTAAAGTTGT+TGG - chr3.4:75116689-75116708 MS.gene074393:intron 25.0%
!!! TGATTGATTTTTCTCTTCTT+TGG - chr3.4:75116287-75116306 MS.gene074393:intron 25.0%
!!! TTCACCATCTTTTTTGTTAT+AGG - chr3.4:75116133-75116152 MS.gene074393:CDS 25.0%
!!! TTGTTGTTTAATTTTTAGCC+AGG - chr3.4:75117109-75117128 MS.gene074393:intron 25.0%
!!! TTTAATTTTTAGCCAGGATT+TGG - chr3.4:75117115-75117134 MS.gene074393:intron 25.0%
ACGAAATGCAAAGTAGTAAA+TGG + chr3.4:75116099-75116118 None:intergenic 30.0%
CATCATATCATTTCTCAGAT+AGG + chr3.4:75116422-75116441 None:intergenic 30.0%
TATCCGATTAGTAAACATGA+TGG - chr3.4:75116370-75116389 MS.gene074393:intron 30.0%
!!! TTGTTCTTTTGATGGGTTTA+GGG - chr3.4:75117166-75117185 MS.gene074393:CDS 30.0%
!!! TTTGTTCTTTTGATGGGTTT+AGG - chr3.4:75117165-75117184 MS.gene074393:CDS 30.0%
AAATCCATGATATGTTTGCC+TGG - chr3.4:75116974-75116993 MS.gene074393:intron 35.0%
AACTTAAAAGAGTGGCATCA+TGG - chr3.4:75116894-75116913 MS.gene074393:intron 35.0%
ACTCATGTATCTGGACTAAA+TGG - chr3.4:75116558-75116577 MS.gene074393:intron 35.0%
GACAAATCAGCTTCAATCAT+CGG + chr3.4:75116633-75116652 None:intergenic 35.0%
GATGTTACGAACAAGTGAAA+AGG + chr3.4:75116204-75116223 None:intergenic 35.0%
TGCATTTCGTAAGAGAGTTA+GGG - chr3.4:75116109-75116128 MS.gene074393:CDS 35.0%
TGGCAAAATACAGATCGAAA+AGG + chr3.4:75116079-75116098 None:intergenic 35.0%
TTGCATTTCGTAAGAGAGTT+AGG - chr3.4:75116108-75116127 MS.gene074393:CDS 35.0%
! GAGTTTGACTTATCTCAGAT+CGG - chr3.4:75116309-75116328 MS.gene074393:intron 35.0%
! TGGTGTTTGAAGGAGTATAA+TGG - chr3.4:75117135-75117154 MS.gene074393:intron 35.0%
AAACATCAGTACCTGACTTC+AGG + chr3.4:75116236-75116255 None:intergenic 40.0%
AACATCAGTACCTGACTTCA+GGG + chr3.4:75116235-75116254 None:intergenic 40.0%
AAGTCGCTTATAGAGAGAGT+AGG + chr3.4:75116514-75116533 None:intergenic 40.0%
CTGGTTGAGTGAAATGAAGT+CGG - chr3.4:75116993-75117012 MS.gene074393:intron 40.0%
TCAACCAGGCAAACATATCA+TGG + chr3.4:75116981-75117000 None:intergenic 40.0%
! AGTACACAGAACATTTGCTG+AGG - chr3.4:75117243-75117262 MS.gene074393:intron 40.0%
AGATCACCAGAGATTATGCC+CGG - chr3.4:75117281-75117300 MS.gene074393:intron 45.0%
AGATCGAAAAGGACTCACCT+CGG + chr3.4:75116068-75116087 None:intergenic 45.0%
CGACTTCATTTCACTCAACC+AGG + chr3.4:75116995-75117014 None:intergenic 45.0%
CTCCTTCAAACACCAAATCC+TGG + chr3.4:75117130-75117149 None:intergenic 45.0%
TTCTGTGTACTTCTCTCCCT+CGG + chr3.4:75117234-75117253 None:intergenic 45.0%
!! AGCCAGGATTTGGTGTTTGA+AGG - chr3.4:75117125-75117144 MS.gene074393:intron 45.0%
AGTGAAGAAGCTGTTGAGGC+CGG + chr3.4:75117303-75117322 None:intergenic 50.0%
GATCGAAAAGGACTCACCTC+GGG + chr3.4:75116067-75116086 None:intergenic 50.0%
GGTCAGTGAAGAAGCTGTTG+AGG + chr3.4:75117307-75117326 None:intergenic 50.0%
GTGTTGGAGAACTTTGCCGA+GGG - chr3.4:75117215-75117234 MS.gene074393:intron 50.0%
TCTGCATCAACCCTGAAGTC+AGG - chr3.4:75116222-75116241 MS.gene074393:intron 50.0%
! AGTGTTGGAGAACTTTGCCG+AGG - chr3.4:75117214-75117233 MS.gene074393:intron 50.0%
! GGTATACCCTCTGTTTGCTG+CGG - chr3.4:75116154-75116173 MS.gene074393:CDS 50.0%
AACAAGCAGTCTCGAGCTGC+AGG - chr3.4:75117191-75117210 MS.gene074393:CDS 55.0%
CTCCAACCGCAGCAAACAGA+GGG + chr3.4:75116163-75116182 None:intergenic 55.0%
GTGAAGAAGCTGTTGAGGCC+GGG + chr3.4:75117302-75117321 None:intergenic 55.0%
TTGAGGCCGGGCATAATCTC+TGG + chr3.4:75117290-75117309 None:intergenic 55.0%
! TACCCTCTGTTTGCTGCGGT+TGG - chr3.4:75116158-75116177 MS.gene074393:CDS 55.0%
CAATCGCTGGATCAGACCCG+AGG - chr3.4:75116048-75116067 MS.gene074393:CDS 60.0%
CTCGGGTCTGATCCAGCGAT+TGG + chr3.4:75116050-75116069 None:intergenic 60.0%
CTGCGGTTGGAGCTGCTGTT+GGG - chr3.4:75116171-75116190 MS.gene074393:CDS 60.0%
GCTCCAACCGCAGCAAACAG+AGG + chr3.4:75116164-75116183 None:intergenic 60.0%
GGGTCTGATCCAGCGATTGG+TGG + chr3.4:75116047-75116066 None:intergenic 60.0%
! GTCTCGAGCTGCAGGAGTGT+TGG - chr3.4:75117199-75117218 MS.gene074393:CDS 60.0%
GCTGCGGTTGGAGCTGCTGT+TGG - chr3.4:75116170-75116189 MS.gene074393:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 75116040 75117340 75116040 ID=MS.gene074393
chr3.4 mRNA 75116040 75117340 75116040 ID=MS.gene074393.t1;Parent=MS.gene074393
chr3.4 exon 75117311 75117340 75117311 ID=MS.gene074393.t1.exon1;Parent=MS.gene074393.t1
chr3.4 CDS 75117311 75117340 75117311 ID=cds.MS.gene074393.t1;Parent=MS.gene074393.t1
chr3.4 exon 75117137 75117225 75117137 ID=MS.gene074393.t1.exon2;Parent=MS.gene074393.t1
chr3.4 CDS 75117137 75117225 75117137 ID=cds.MS.gene074393.t1;Parent=MS.gene074393.t1
chr3.4 exon 75116040 75116193 75116040 ID=MS.gene074393.t1.exon3;Parent=MS.gene074393.t1
chr3.4 CDS 75116040 75116193 75116040 ID=cds.MS.gene074393.t1;Parent=MS.gene074393.t1
Gene Sequence

>MS.gene074393

ATGGCCACCAATCGCTGGATCAGACCCGAGGTGAGTCCTTTTCGATCTGTATTTTGCCATTTACTACTTTGCATTTCGTAAGAGAGTTAGGGTTTCACCATCTTTTTTGTTATAGGTATACCCTCTGTTTGCTGCGGTTGGAGCTGCTGTTGGGATCTGTGCCTTTTCACTTGTTCGTAACATCTGCATCAACCCTGAAGTCAGGTACTGATGTTTTTTTATTTTTTTATTTTATCTTTGAGATTATTGATTGATTTTTCTCTTCTTTGGAGTTTGACTTATCTCAGATCGGTGATTAATACTGCTAATAATTGCTTTCCATTAATTAATTATCCGATTAGTAAACATGATGGAATATTCCTTTTTTATTTTTGGTTTTCCTATCTGAGAAATGATATGATGTATCCGCTTAAATTTTCACAATTTGTTTCTAGATATTAACAAAATTTAATGGGATTCTATTTTTTGATACCTACTCTCTCTATAAGCGACTTTTTTTTTTTTTTCCAAATTAAATAACTCATGTATCTGGACTAAATGGATTTGATTAACTAAGTGATTTTTAGGAGATCTTTAACTATGATAATGATCCGATGATTGAAGCTGATTTGTCAAATTTATTAAAGCGAATTTTGCACAAATAACGTTTAATGGTGATTTTAAAGTTGTTGGCTACCCTATTTGATATTAGAGATTAATTTTTATTCATTGGAGGTGTAAATTTTGTTTTACACGTGCATCTAATCAAATCACGTCAAACTGATATTAGAGATTAATTTTTATTCATTGGAGGTGTAAATTTTGTTTTACACGTGCATCTAATCAAATCACGTCAAACTGATATTTTGAGATATAACTTAAAAGAGTGGCATCATGGTGTGATTTTATTGAATGCATCTGTAAAAATGTTTTACACTGTCAGTGTATACAAGTTAAATCCATGATATGTTTGCCTGGTTGAGTGAAATGAAGTCGGAGATATTCAATTGCTTGAGATTATTTAAATTATGTCTTGACAGAACTGCTATCTATTGAAAATGAAAACTTTTATCTCACAGGGATGTAGCTATTGTTGTTTAATTTTTAGCCAGGATTTGGTGTTTGAAGGAGTATAATGGATGTATGTTTGTTCTTTTGATGGGTTTAGGGTGAACAAGCAGTCTCGAGCTGCAGGAGTGTTGGAGAACTTTGCCGAGGGAGAGAAGTACACAGAACATTTGCTGAGGAAGTTTTCACGCAACAGATCACCAGAGATTATGCCCGGCCTCAACAGCTTCTTCACTGACCCAAGTCGCAACTAA

Protein sequence

>MS.gene074393.t1

MATNRWIRPEVYPLFAAVGAAVGICAFSLVRNICINPEVRVNKQSRAAGVLENFAEGEKYTEHLLRKFSRNRSPEIMPGLNSFFTDPSRN