Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70021.t1 | KEH35118.1 | 89.6 | 501 | 40 | 3 | 1 | 489 | 170 | 670 | 4.50E-250 | 873.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70021.t1 | Q40392 | 37.7 | 533 | 303 | 9 | 1 | 517 | 161 | 680 | 8.4e-88 | 325.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70021.t1 | A0A072V165 | 89.6 | 501 | 40 | 3 | 1 | 489 | 170 | 670 | 3.2e-250 | 873.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene70021 | 0.843691 | 1.20E-58 | -1.69E-46 |
| MS.gene049151 | MS.gene70021 | 0.808184 | 3.51E-50 | -1.69E-46 |
| MS.gene049153 | MS.gene70021 | 0.818296 | 2.11E-52 | -1.69E-46 |
| MS.gene049159 | MS.gene70021 | 0.818886 | 1.55E-52 | -1.69E-46 |
| MS.gene049297 | MS.gene70021 | 0.811225 | 7.79E-51 | -1.69E-46 |
| MS.gene049378 | MS.gene70021 | 0.874951 | 4.52E-68 | -1.69E-46 |
| MS.gene049435 | MS.gene70021 | 0.83306 | 6.62E-56 | -1.69E-46 |
| MS.gene049669 | MS.gene70021 | 0.830069 | 3.61E-55 | -1.69E-46 |
| MS.gene050020 | MS.gene70021 | 0.800336 | 1.51E-48 | -1.69E-46 |
| MS.gene050139 | MS.gene70021 | 0.815857 | 7.46E-52 | -1.69E-46 |
| MS.gene050168 | MS.gene70021 | 0.821515 | 3.87E-53 | -1.69E-46 |
| MS.gene050226 | MS.gene70021 | 0.873496 | 1.41E-67 | -1.69E-46 |
| MS.gene050227 | MS.gene70021 | 0.869803 | 2.35E-66 | -1.69E-46 |
| MS.gene050228 | MS.gene70021 | 0.874853 | 4.88E-68 | -1.69E-46 |
| MS.gene050330 | MS.gene70021 | -0.811988 | 5.31E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene70021 | 0.866504 | 2.72E-65 | -1.69E-46 |
| MS.gene050586 | MS.gene70021 | 0.800949 | 1.13E-48 | -1.69E-46 |
| MS.gene050605 | MS.gene70021 | 0.82174 | 3.44E-53 | -1.69E-46 |
| MS.gene050623 | MS.gene70021 | -0.849992 | 2.26E-60 | -1.69E-46 |
| MS.gene050642 | MS.gene70021 | 0.839131 | 1.90E-57 | -1.69E-46 |
| MS.gene050680 | MS.gene70021 | 0.839176 | 1.85E-57 | -1.69E-46 |
| MS.gene050719 | MS.gene70021 | 0.820107 | 8.17E-53 | -1.69E-46 |
| MS.gene050721 | MS.gene70021 | 0.817527 | 3.15E-52 | -1.69E-46 |
| MS.gene050724 | MS.gene70021 | 0.813808 | 2.12E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene70021 | 0.837075 | 6.44E-57 | -1.69E-46 |
| MS.gene050762 | MS.gene70021 | 0.821564 | 3.78E-53 | -1.69E-46 |
| MS.gene050763 | MS.gene70021 | 0.821224 | 4.52E-53 | -1.69E-46 |
| MS.gene050780 | MS.gene70021 | 0.850863 | 1.29E-60 | -1.69E-46 |
| MS.gene050807 | MS.gene70021 | 0.825191 | 5.36E-54 | -1.69E-46 |
| MS.gene050820 | MS.gene70021 | 0.844734 | 6.29E-59 | -1.69E-46 |
| MS.gene050826 | MS.gene70021 | 0.810949 | 8.94E-51 | -1.69E-46 |
| MS.gene050953 | MS.gene70021 | 0.846963 | 1.56E-59 | -1.69E-46 |
| MS.gene050987 | MS.gene70021 | 0.832632 | 8.46E-56 | -1.69E-46 |
| MS.gene051265 | MS.gene70021 | 0.819746 | 9.88E-53 | -1.69E-46 |
| MS.gene051860 | MS.gene70021 | 0.823772 | 1.16E-53 | -1.69E-46 |
| MS.gene051935 | MS.gene70021 | 0.813732 | 2.21E-51 | -1.69E-46 |
| MS.gene051983 | MS.gene70021 | 0.847024 | 1.50E-59 | -1.69E-46 |
| MS.gene052012 | MS.gene70021 | 0.811727 | 6.06E-51 | -1.69E-46 |
| MS.gene05215 | MS.gene70021 | 0.811605 | 6.44E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene70021 | 0.8513 | 9.71E-61 | -1.69E-46 |
| MS.gene052333 | MS.gene70021 | 0.848208 | 7.09E-60 | -1.69E-46 |
| MS.gene052376 | MS.gene70021 | 0.8199 | 9.11E-53 | -1.69E-46 |
| MS.gene052389 | MS.gene70021 | 0.840866 | 6.72E-58 | -1.69E-46 |
| MS.gene052426 | MS.gene70021 | 0.845742 | 3.36E-59 | -1.69E-46 |
| MS.gene052586 | MS.gene70021 | 0.819608 | 1.06E-52 | -1.69E-46 |
| MS.gene053074 | MS.gene70021 | 0.82781 | 1.27E-54 | -1.69E-46 |
| MS.gene053147 | MS.gene70021 | 0.809179 | 2.15E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene70021 | 0.866648 | 2.44E-65 | -1.69E-46 |
| MS.gene053184 | MS.gene70021 | 0.828808 | 7.32E-55 | -1.69E-46 |
| MS.gene053494 | MS.gene70021 | 0.831553 | 1.56E-55 | -1.69E-46 |
| MS.gene053584 | MS.gene70021 | 0.826092 | 3.28E-54 | -1.69E-46 |
| MS.gene053836 | MS.gene70021 | 0.82346 | 1.37E-53 | -1.69E-46 |
| MS.gene054083 | MS.gene70021 | 0.827505 | 1.51E-54 | -1.69E-46 |
| MS.gene05414 | MS.gene70021 | 0.844748 | 6.24E-59 | -1.69E-46 |
| MS.gene05415 | MS.gene70021 | 0.841102 | 5.82E-58 | -1.69E-46 |
| MS.gene05417 | MS.gene70021 | 0.803301 | 3.72E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene70021 | 0.828007 | 1.14E-54 | -1.69E-46 |
| MS.gene054236 | MS.gene70021 | 0.822701 | 2.06E-53 | -1.69E-46 |
| MS.gene054291 | MS.gene70021 | -0.838632 | 2.56E-57 | -1.69E-46 |
| MS.gene054379 | MS.gene70021 | 0.837061 | 6.49E-57 | -1.69E-46 |
| MS.gene054405 | MS.gene70021 | 0.833899 | 4.09E-56 | -1.69E-46 |
| MS.gene054467 | MS.gene70021 | 0.842684 | 2.23E-58 | -1.69E-46 |
| MS.gene054468 | MS.gene70021 | 0.873951 | 9.87E-68 | -1.69E-46 |
| MS.gene054542 | MS.gene70021 | 0.805206 | 1.49E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene70021 | 0.821667 | 3.58E-53 | -1.69E-46 |
| MS.gene054576 | MS.gene70021 | 0.804561 | 2.04E-49 | -1.69E-46 |
| MS.gene054596 | MS.gene70021 | 0.817411 | 3.34E-52 | -1.69E-46 |
| MS.gene054657 | MS.gene70021 | 0.822807 | 1.94E-53 | -1.69E-46 |
| MS.gene05468 | MS.gene70021 | 0.82255 | 2.23E-53 | -1.69E-46 |
| MS.gene054826 | MS.gene70021 | 0.814335 | 1.62E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene70021 | 0.842035 | 3.31E-58 | -1.69E-46 |
| MS.gene054942 | MS.gene70021 | 0.825821 | 3.80E-54 | -1.69E-46 |
| MS.gene05510 | MS.gene70021 | 0.843044 | 1.78E-58 | -1.69E-46 |
| MS.gene055227 | MS.gene70021 | 0.803866 | 2.84E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene70021 | 0.849378 | 3.36E-60 | -1.69E-46 |
| MS.gene055357 | MS.gene70021 | 0.825096 | 5.65E-54 | -1.69E-46 |
| MS.gene055358 | MS.gene70021 | 0.858087 | 1.05E-62 | -1.69E-46 |
| MS.gene055367 | MS.gene70021 | 0.813409 | 2.60E-51 | -1.69E-46 |
| MS.gene055604 | MS.gene70021 | 0.804593 | 2.01E-49 | -1.69E-46 |
| MS.gene055623 | MS.gene70021 | 0.809654 | 1.70E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene70021 | 0.889656 | 1.99E-73 | -1.69E-46 |
| MS.gene055818 | MS.gene70021 | 0.818107 | 2.33E-52 | -1.69E-46 |
| MS.gene055926 | MS.gene70021 | 0.833327 | 5.69E-56 | -1.69E-46 |
| MS.gene05604 | MS.gene70021 | 0.861599 | 9.16E-64 | -1.69E-46 |
| MS.gene056173 | MS.gene70021 | 0.805803 | 1.12E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene70021 | 0.874552 | 6.18E-68 | -1.69E-46 |
| MS.gene056552 | MS.gene70021 | 0.808789 | 2.61E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene70021 | 0.840624 | 7.77E-58 | -1.69E-46 |
| MS.gene057012 | MS.gene70021 | 0.827335 | 1.66E-54 | -1.69E-46 |
| MS.gene057026 | MS.gene70021 | 0.85672 | 2.66E-62 | -1.69E-46 |
| MS.gene057060 | MS.gene70021 | 0.828601 | 8.21E-55 | -1.69E-46 |
| MS.gene057156 | MS.gene70021 | -0.833357 | 5.59E-56 | -1.69E-46 |
| MS.gene057200 | MS.gene70021 | 0.806014 | 1.01E-49 | -1.69E-46 |
| MS.gene057394 | MS.gene70021 | 0.826931 | 2.07E-54 | -1.69E-46 |
| MS.gene057395 | MS.gene70021 | 0.826237 | 3.03E-54 | -1.69E-46 |
| MS.gene057469 | MS.gene70021 | 0.81692 | 4.31E-52 | -1.69E-46 |
| MS.gene05761 | MS.gene70021 | 0.827083 | 1.90E-54 | -1.69E-46 |
| MS.gene057639 | MS.gene70021 | 0.815435 | 9.26E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene70021 | 0.815922 | 7.21E-52 | -1.69E-46 |
| MS.gene057750 | MS.gene70021 | 0.834591 | 2.74E-56 | -1.69E-46 |
| MS.gene057869 | MS.gene70021 | 0.82851 | 8.64E-55 | -1.69E-46 |
| MS.gene057915 | MS.gene70021 | 0.805475 | 1.31E-49 | -1.69E-46 |
| MS.gene057929 | MS.gene70021 | 0.827183 | 1.80E-54 | -1.69E-46 |
| MS.gene058139 | MS.gene70021 | 0.834649 | 2.65E-56 | -1.69E-46 |
| MS.gene058142 | MS.gene70021 | 0.812989 | 3.21E-51 | -1.69E-46 |
| MS.gene058146 | MS.gene70021 | 0.846424 | 2.19E-59 | -1.69E-46 |
| MS.gene058168 | MS.gene70021 | 0.836635 | 8.33E-57 | -1.69E-46 |
| MS.gene058220 | MS.gene70021 | 0.823909 | 1.07E-53 | -1.69E-46 |
| MS.gene058378 | MS.gene70021 | 0.811289 | 7.54E-51 | -1.69E-46 |
| MS.gene058426 | MS.gene70021 | 0.814589 | 1.43E-51 | -1.69E-46 |
| MS.gene058435 | MS.gene70021 | 0.815684 | 8.15E-52 | -1.69E-46 |
| MS.gene058548 | MS.gene70021 | 0.800337 | 1.51E-48 | -1.69E-46 |
| MS.gene058595 | MS.gene70021 | 0.81326 | 2.80E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene70021 | 0.807406 | 5.13E-50 | -1.69E-46 |
| MS.gene059006 | MS.gene70021 | 0.803391 | 3.56E-49 | -1.69E-46 |
| MS.gene059124 | MS.gene70021 | 0.821674 | 3.56E-53 | -1.69E-46 |
| MS.gene059128 | MS.gene70021 | 0.840402 | 8.89E-58 | -1.69E-46 |
| MS.gene059160 | MS.gene70021 | 0.807625 | 4.61E-50 | -1.69E-46 |
| MS.gene059161 | MS.gene70021 | 0.871904 | 4.79E-67 | -1.69E-46 |
| MS.gene059162 | MS.gene70021 | 0.821207 | 4.56E-53 | -1.69E-46 |
| MS.gene059247 | MS.gene70021 | 0.815299 | 9.93E-52 | -1.69E-46 |
| MS.gene059550 | MS.gene70021 | 0.804544 | 2.05E-49 | -1.69E-46 |
| MS.gene059609 | MS.gene70021 | 0.841706 | 4.04E-58 | -1.69E-46 |
| MS.gene059725 | MS.gene70021 | 0.806947 | 6.42E-50 | -1.69E-46 |
| MS.gene05973 | MS.gene70021 | 0.819135 | 1.36E-52 | -1.69E-46 |
| MS.gene060137 | MS.gene70021 | -0.826136 | 3.20E-54 | -1.69E-46 |
| MS.gene060167 | MS.gene70021 | 0.80431 | 2.30E-49 | -1.69E-46 |
| MS.gene060246 | MS.gene70021 | 0.802022 | 6.83E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene70021 | 0.853666 | 2.05E-61 | -1.69E-46 |
| MS.gene060373 | MS.gene70021 | 0.803361 | 3.62E-49 | -1.69E-46 |
| MS.gene06052 | MS.gene70021 | 0.811225 | 7.79E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene70021 | 0.81837 | 2.03E-52 | -1.69E-46 |
| MS.gene060683 | MS.gene70021 | 0.848774 | 4.95E-60 | -1.69E-46 |
| MS.gene060685 | MS.gene70021 | 0.84514 | 4.89E-59 | -1.69E-46 |
| MS.gene060802 | MS.gene70021 | 0.844912 | 5.63E-59 | -1.69E-46 |
| MS.gene060803 | MS.gene70021 | 0.837849 | 4.08E-57 | -1.69E-46 |
| MS.gene060879 | MS.gene70021 | 0.808455 | 3.07E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene70021 | 0.866799 | 2.19E-65 | -1.69E-46 |
| MS.gene061040 | MS.gene70021 | -0.835614 | 1.51E-56 | -1.69E-46 |
| MS.gene061085 | MS.gene70021 | 0.809248 | 2.08E-50 | -1.69E-46 |
| MS.gene061114 | MS.gene70021 | -0.842831 | 2.03E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 90 sgRNAs with CRISPR-Local
Find 131 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTTGGCTTTAAAAGTTT+TGG | 0.160312 | 3.4:-75641951 | MS.gene70021:CDS |
| GACATAGCATGTTTCGTTAA+AGG | 0.173648 | 3.4:-75641788 | MS.gene70021:CDS |
| AGATATCTTAAATTCCATTA+TGG | 0.208837 | 3.4:-75641357 | MS.gene70021:CDS |
| GACTTCTGCTTCAGGGTCTT+TGG | 0.227515 | 3.4:+75641595 | None:intergenic |
| TTTGTCTATGGCTAAGAATT+TGG | 0.250461 | 3.4:-75640741 | MS.gene70021:CDS |
| AAGTTAGAGTGATTGGGATA+TGG | 0.252341 | 3.4:-75642471 | MS.gene70021:CDS |
| TCCATTATGGGACGTGCAAC+GGG | 0.289766 | 3.4:-75641344 | MS.gene70021:CDS |
| TGTTAAATCATTATTAGAAA+TGG | 0.299353 | 3.4:-75642503 | MS.gene70021:CDS |
| TCAAAAGAAGTTAGAGTGAT+TGG | 0.326042 | 3.4:-75642478 | MS.gene70021:CDS |
| TCAGAATCTTGTGAATTTGA+AGG | 0.331834 | 3.4:-75640807 | MS.gene70021:intron |
| CACAACTCCCTTCAATTCAA+TGG | 0.332796 | 3.4:+75642549 | None:intergenic |
| ACTTCTGCTTCAGGGTCTTT+GGG | 0.333053 | 3.4:+75641596 | None:intergenic |
| ACATAACGGTATTCAACTTT+GGG | 0.342163 | 3.4:+75642265 | None:intergenic |
| CCCCGTTGCACGTCCCATAA+TGG | 0.344539 | 3.4:+75641343 | None:intergenic |
| AAAGCTTTGGGAAGGAGTTC+AGG | 0.344711 | 3.4:-75641175 | MS.gene70021:intron |
| CCACAATCGACTTCTGCTTC+AGG | 0.345005 | 3.4:+75641587 | None:intergenic |
| ACAGCAACCTTCAAAAGCTT+TGG | 0.347076 | 3.4:-75641188 | MS.gene70021:CDS |
| GACATAACGGTATTCAACTT+TGG | 0.349208 | 3.4:+75642264 | None:intergenic |
| TTCAAATTGAGAAAATAGTT+TGG | 0.351857 | 3.4:+75642402 | None:intergenic |
| GCACGATTTGATACAAGAAA+TGG | 0.355253 | 3.4:-75641645 | MS.gene70021:CDS |
| GATATCTTAAATTCCATTAT+GGG | 0.355934 | 3.4:-75641356 | MS.gene70021:CDS |
| AGAAAGCTCTACAAGCAATT+TGG | 0.356833 | 3.4:+75641218 | None:intergenic |
| ATCAGTGATTATGAATGTTT+CGG | 0.364057 | 3.4:-75642166 | MS.gene70021:CDS |
| CAGCAACCTTCAAAAGCTTT+GGG | 0.370596 | 3.4:-75641187 | MS.gene70021:CDS |
| AATACCGTTATGTCAACAAT+AGG | 0.378686 | 3.4:-75642255 | MS.gene70021:CDS |
| TGTAATTTCTTCCCGGGCAT+TGG | 0.384508 | 3.4:-75641728 | MS.gene70021:CDS |
| TGGTACCTCAATCAGCTCTT+TGG | 0.392162 | 3.4:+75640766 | None:intergenic |
| GAAAGCTTGTAATTTCTTCC+CGG | 0.398081 | 3.4:-75641735 | MS.gene70021:CDS |
| AGTTAGAGTGATTGGGATAT+GGG | 0.418311 | 3.4:-75642470 | MS.gene70021:CDS |
| CAGGGAACTGTAGCGGTAGA+AGG | 0.418809 | 3.4:-75641453 | MS.gene70021:CDS |
| TGAGGTACCAGATTTGTCTA+TGG | 0.422376 | 3.4:-75640753 | MS.gene70021:CDS |
| GTTAAATCATTATTAGAAAT+GGG | 0.422516 | 3.4:-75642502 | MS.gene70021:CDS |
| TTTAGATGTGTCTAAAATTG+AGG | 0.439446 | 3.4:-75641424 | MS.gene70021:CDS |
| TTGCACAACCTTCCAGTTCG+AGG | 0.439476 | 3.4:+75640642 | None:intergenic |
| ATATGGGGCATGGGTGGCAT+AGG | 0.440029 | 3.4:-75642454 | MS.gene70021:CDS |
| AATTTATCTTCCCAACGGTC+TGG | 0.443557 | 3.4:-75641316 | MS.gene70021:CDS |
| TATCGTACCCTTGTTACAAA+TGG | 0.447573 | 3.4:+75641673 | None:intergenic |
| GAAGGGAGTTGTGGGAATTG+AGG | 0.450294 | 3.4:-75642539 | MS.gene70021:CDS |
| CACAATCGACTTCTGCTTCA+GGG | 0.459131 | 3.4:+75641588 | None:intergenic |
| CAAAAGAAGTTAGAGTGATT+GGG | 0.465079 | 3.4:-75642477 | MS.gene70021:CDS |
| GTTACAATCACTCTACTTCC+TGG | 0.465368 | 3.4:+75642142 | None:intergenic |
| ACAACTCCCTTCAATTCAAT+GGG | 0.465850 | 3.4:+75642550 | None:intergenic |
| AAAGAAAAGCATCCTAAAAG+TGG | 0.467941 | 3.4:-75642019 | MS.gene70021:CDS |
| GACAATGATTCCAGACCGTT+GGG | 0.475225 | 3.4:+75641306 | None:intergenic |
| TTCCATTATGGGACGTGCAA+CGG | 0.475392 | 3.4:-75641345 | MS.gene70021:CDS |
| ATTGAATTGAAGGGAGTTGT+GGG | 0.477503 | 3.4:-75642547 | MS.gene70021:CDS |
| TTCTTAGCCATAGACAAATC+TGG | 0.481065 | 3.4:+75640746 | None:intergenic |
| TGGCATGTAAAGCAATTGCT+AGG | 0.481673 | 3.4:+75642422 | None:intergenic |
| ACAAATTGAGGTACATTGAA+TGG | 0.482299 | 3.4:-75641281 | MS.gene70021:CDS |
| ATATTGCAAAGGCAATCCTT+TGG | 0.482877 | 3.4:-75641966 | MS.gene70021:CDS |
| CGTACAACCCATTGAATTGA+AGG | 0.484059 | 3.4:-75642557 | MS.gene70021:CDS |
| AGGTCATCTATTCCAATGCC+CGG | 0.494391 | 3.4:+75641716 | None:intergenic |
| CACGATTTGATACAAGAAAT+GGG | 0.497036 | 3.4:-75641644 | MS.gene70021:CDS |
| ATTTGATACAAGAAATGGGC+TGG | 0.505777 | 3.4:-75641640 | MS.gene70021:CDS |
| CATTGAATTGAAGGGAGTTG+TGG | 0.520229 | 3.4:-75642548 | MS.gene70021:CDS |
| GATTATGAATGTTTCGGACC+AGG | 0.524245 | 3.4:-75642160 | MS.gene70021:CDS |
| AATCATTGTCTGACAAATTG+AGG | 0.526906 | 3.4:-75641293 | MS.gene70021:CDS |
| CAAGCTTCTAGACCTCGAAC+TGG | 0.528502 | 3.4:-75640654 | MS.gene70021:CDS |
| AGACAATGATTCCAGACCGT+TGG | 0.530649 | 3.4:+75641305 | None:intergenic |
| CTGAAGCAGAAGTCGATTGT+GGG | 0.532002 | 3.4:-75641586 | MS.gene70021:CDS |
| AAAGCTTGTAATTTCTTCCC+GGG | 0.534180 | 3.4:-75641734 | MS.gene70021:CDS |
| AGTGATTGGGATATGGGGCA+TGG | 0.536168 | 3.4:-75642464 | MS.gene70021:CDS |
| CAGTACATCATACACGTCGT+TGG | 0.540699 | 3.4:+75641561 | None:intergenic |
| CCTGAAGCAGAAGTCGATTG+TGG | 0.541710 | 3.4:-75641587 | MS.gene70021:CDS |
| AAGCTTGTCGAGTGACAGAA+TGG | 0.546852 | 3.4:+75640670 | None:intergenic |
| TCTATGGCTAAGAATTTGGA+AGG | 0.553862 | 3.4:-75640737 | MS.gene70021:CDS |
| AACCTTCAAAAGCTTTGGGA+AGG | 0.554067 | 3.4:-75641183 | MS.gene70021:CDS |
| GTGATTGGGATATGGGGCAT+GGG | 0.554209 | 3.4:-75642463 | MS.gene70021:CDS |
| TATGGGGCATGGGTGGCATA+GGG | 0.556916 | 3.4:-75642453 | MS.gene70021:CDS |
| ATTCTCCAAAGAGCTGATTG+AGG | 0.572305 | 3.4:-75640771 | MS.gene70021:CDS |
| TAAGCCTATTGTTGACATAA+CGG | 0.572696 | 3.4:+75642251 | None:intergenic |
| TGATGAAATATATGAGGTCA+AGG | 0.575948 | 3.4:-75642089 | MS.gene70021:CDS |
| AGTGTAATTGCATATTGCAA+AGG | 0.579149 | 3.4:-75641977 | MS.gene70021:CDS |
| GTTCAAGAAGTGAAGAAGCA+TGG | 0.579983 | 3.4:-75641916 | MS.gene70021:CDS |
| ATTGGGATATGGGGCATGGG+TGG | 0.583261 | 3.4:-75642460 | MS.gene70021:CDS |
| CGAGTGACAGAATGGAAGGA+TGG | 0.584763 | 3.4:+75640678 | None:intergenic |
| TCTTGTAACCATTTGTAACA+AGG | 0.585164 | 3.4:-75641681 | MS.gene70021:CDS |
| CAATGTTGATGAAATATATG+AGG | 0.585813 | 3.4:-75642095 | MS.gene70021:CDS |
| ATGTAAAGCAATTGCTAGGG+TGG | 0.586146 | 3.4:+75642426 | None:intergenic |
| CTTGTAACCATTTGTAACAA+GGG | 0.592703 | 3.4:-75641680 | MS.gene70021:CDS |
| GGTCATCTATTCCAATGCCC+GGG | 0.593863 | 3.4:+75641717 | None:intergenic |
| TTGTCGAGTGACAGAATGGA+AGG | 0.601159 | 3.4:+75640674 | None:intergenic |
| GGCATGTAAAGCAATTGCTA+GGG | 0.604801 | 3.4:+75642423 | None:intergenic |
| GGAATTGAGGAAAACTATGA+AGG | 0.608522 | 3.4:-75642526 | MS.gene70021:CDS |
| GTTAGAGTGATTGGGATATG+GGG | 0.623247 | 3.4:-75642469 | MS.gene70021:CDS |
| GCAAGAATTTATCTTCCCAA+CGG | 0.623755 | 3.4:-75641321 | MS.gene70021:CDS |
| CTTCTAGACCTCGAACTGGA+AGG | 0.624025 | 3.4:-75640650 | MS.gene70021:CDS |
| GTACAACCCATTGAATTGAA+GGG | 0.651082 | 3.4:-75642556 | MS.gene70021:CDS |
| TATTTGTCAGGGAACTGTAG+CGG | 0.652760 | 3.4:-75641460 | MS.gene70021:intron |
| CCATTATGGGACGTGCAACG+GGG | 0.723491 | 3.4:-75641343 | MS.gene70021:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAAACATTATAAAAAATT+TGG | - | chr3.4:75642170-75642189 | MS.gene70021:CDS | 10.0% |
| !! | AGAAAAAAAAAACATAAATG+TGG | + | chr3.4:75642418-75642437 | None:intergenic | 15.0% |
| !! | GTTAAATCATTATTAGAAAT+GGG | - | chr3.4:75640745-75640764 | MS.gene70021:CDS | 15.0% |
| !! | TCAATTAGAAAATTAAACAA+GGG | - | chr3.4:75642281-75642300 | MS.gene70021:CDS | 15.0% |
| !! | TGTCTATTTAAAAACTATAA+GGG | - | chr3.4:75642311-75642330 | MS.gene70021:CDS | 15.0% |
| !! | TGTTAAATCATTATTAGAAA+TGG | - | chr3.4:75640744-75640763 | MS.gene70021:CDS | 15.0% |
| !!! | TAAAAAAGTTCTTATTGTTT+TGG | - | chr3.4:75641023-75641042 | MS.gene70021:intron | 15.0% |
| !!! | TAATGAATTTTTATTTGTCA+GGG | - | chr3.4:75641776-75641795 | MS.gene70021:CDS | 15.0% |
| !!! | TTAATGAATTTTTATTTGTC+AGG | - | chr3.4:75641775-75641794 | MS.gene70021:CDS | 15.0% |
| !! | AGATATCTTAAATTCCATTA+TGG | - | chr3.4:75641890-75641909 | MS.gene70021:CDS | 20.0% |
| !! | GATATCTTAAATTCCATTAT+GGG | - | chr3.4:75641891-75641910 | MS.gene70021:CDS | 20.0% |
| !! | GTCAATTAGAAAATTAAACA+AGG | - | chr3.4:75642280-75642299 | MS.gene70021:CDS | 20.0% |
| !! | GTCTATTTAAAAACTATAAG+GGG | - | chr3.4:75642312-75642331 | MS.gene70021:CDS | 20.0% |
| !! | GTGTCTATTTAAAAACTATA+AGG | - | chr3.4:75642310-75642329 | MS.gene70021:CDS | 20.0% |
| !! | TGATGTACTGAAATATAATA+AGG | - | chr3.4:75641699-75641718 | MS.gene70021:CDS | 20.0% |
| !! | TTCAAATTGAGAAAATAGTT+TGG | + | chr3.4:75640848-75640867 | None:intergenic | 20.0% |
| !!! | AAACTATTTTCTCAATTTGA+AGG | - | chr3.4:75640847-75640866 | MS.gene70021:intron | 20.0% |
| ! | ATCAGTGATTATGAATGTTT+CGG | - | chr3.4:75641081-75641100 | MS.gene70021:intron | 25.0% |
| ! | ATTAGAAAATTAAACAAGGG+AGG | - | chr3.4:75642284-75642303 | MS.gene70021:CDS | 25.0% |
| ! | ATTTAAACAAACTTTCAAGG+AGG | - | chr3.4:75642345-75642364 | MS.gene70021:CDS | 25.0% |
| ! | GTCATTTAAACAAACTTTCA+AGG | - | chr3.4:75642342-75642361 | MS.gene70021:CDS | 25.0% |
| ! | TGTTAACATAGGAAATTAAG+AGG | + | chr3.4:75642224-75642243 | None:intergenic | 25.0% |
| !! | AATTTGGCATCAAATTTTGA+TGG | + | chr3.4:75642016-75642035 | None:intergenic | 25.0% |
| !! | ACATTCTGAATTTTCATTTC+TGG | + | chr3.4:75641387-75641406 | None:intergenic | 25.0% |
| !! | CAATGTTGATGAAATATATG+AGG | - | chr3.4:75641152-75641171 | MS.gene70021:intron | 25.0% |
| !! | TTTAGATGTGTCTAAAATTG+AGG | - | chr3.4:75641823-75641842 | MS.gene70021:CDS | 25.0% |
| !!! | TCTTATTGTTTTGGATAATG+TGG | - | chr3.4:75641032-75641051 | MS.gene70021:intron | 25.0% |
| AATACCGTTATGTCAACAAT+AGG | - | chr3.4:75640992-75641011 | MS.gene70021:intron | 30.0% | |
| AATCATTGTCTGACAAATTG+AGG | - | chr3.4:75641954-75641973 | MS.gene70021:CDS | 30.0% | |
| ACAAATTGAGGTACATTGAA+TGG | - | chr3.4:75641966-75641985 | MS.gene70021:CDS | 30.0% | |
| ACATAACGGTATTCAACTTT+GGG | + | chr3.4:75640985-75641004 | None:intergenic | 30.0% | |
| AGTGTAATTGCATATTGCAA+AGG | - | chr3.4:75641270-75641289 | MS.gene70021:CDS | 30.0% | |
| CAAAAGAAGTTAGAGTGATT+GGG | - | chr3.4:75640770-75640789 | MS.gene70021:CDS | 30.0% | |
| CACGATTTGATACAAGAAAT+GGG | - | chr3.4:75641603-75641622 | MS.gene70021:CDS | 30.0% | |
| CATAAATGTGGAATTAGCAA+CGG | + | chr3.4:75642406-75642425 | None:intergenic | 30.0% | |
| CTATGTTAACAGCAAAGAAA+CGG | - | chr3.4:75642233-75642252 | MS.gene70021:CDS | 30.0% | |
| CTTGTAACCATTTGTAACAA+GGG | - | chr3.4:75641567-75641586 | MS.gene70021:intron | 30.0% | |
| TAAGCCTATTGTTGACATAA+CGG | + | chr3.4:75640999-75641018 | None:intergenic | 30.0% | |
| TCAAAAGAAGTTAGAGTGAT+TGG | - | chr3.4:75640769-75640788 | MS.gene70021:CDS | 30.0% | |
| TCAGAATCTTGTGAATTTGA+AGG | - | chr3.4:75642440-75642459 | MS.gene70021:CDS | 30.0% | |
| TCTTGTAACCATTTGTAACA+AGG | - | chr3.4:75641566-75641585 | MS.gene70021:intron | 30.0% | |
| TGATGAAATATATGAGGTCA+AGG | - | chr3.4:75641158-75641177 | MS.gene70021:intron | 30.0% | |
| TTTGTCTATGGCTAAGAATT+TGG | - | chr3.4:75642506-75642525 | MS.gene70021:CDS | 30.0% | |
| ! | AGAAGCATGGAAAAATGAAT+TGG | - | chr3.4:75641344-75641363 | MS.gene70021:CDS | 30.0% |
| ! | CTTTTTACATCAGTTCAACT+GGG | - | chr3.4:75642110-75642129 | MS.gene70021:CDS | 30.0% |
| ! | TCTTTTTACATCAGTTCAAC+TGG | - | chr3.4:75642109-75642128 | MS.gene70021:CDS | 30.0% |
| ! | TTACAAGAGACTTTTCTACA+AGG | + | chr3.4:75641554-75641573 | None:intergenic | 30.0% |
| !! | AAAGAAAAGCATCCTAAAAG+TGG | - | chr3.4:75641228-75641247 | MS.gene70021:CDS | 30.0% |
| !! | ATTTGAAGGTCATTGCTTTT+TGG | - | chr3.4:75640861-75640880 | MS.gene70021:intron | 30.0% |
| !!! | ATTGCTTTTTGGCAAATGTA+AGG | - | chr3.4:75640872-75640891 | MS.gene70021:intron | 30.0% |
| !!! | CCTTTGGCTTTAAAAGTTTT+GGG | - | chr3.4:75641297-75641316 | MS.gene70021:CDS | 30.0% |
| !!! | TCCTTTGGCTTTAAAAGTTT+TGG | - | chr3.4:75641296-75641315 | MS.gene70021:CDS | 30.0% |
| !!! | TTGCTTTTTGGCAAATGTAA+GGG | - | chr3.4:75640873-75640892 | MS.gene70021:intron | 30.0% |
| !!! | TTTTCATTTCTGGAATCTTC+TGG | + | chr3.4:75641377-75641396 | None:intergenic | 30.0% |
| AAAGCTTGTAATTTCTTCCC+GGG | - | chr3.4:75641513-75641532 | MS.gene70021:intron | 35.0% | |
| AAGTTAGAGTGATTGGGATA+TGG | - | chr3.4:75640776-75640795 | MS.gene70021:CDS | 35.0% | |
| ACAACTCCCTTCAATTCAAT+GGG | + | chr3.4:75640700-75640719 | None:intergenic | 35.0% | |
| AGAAAGCTCTACAAGCAATT+TGG | + | chr3.4:75642032-75642051 | None:intergenic | 35.0% | |
| AGTTAGAGTGATTGGGATAT+GGG | - | chr3.4:75640777-75640796 | MS.gene70021:CDS | 35.0% | |
| ATATTGCAAAGGCAATCCTT+TGG | - | chr3.4:75641281-75641300 | MS.gene70021:CDS | 35.0% | |
| ATTGAATTGAAGGGAGTTGT+GGG | - | chr3.4:75640700-75640719 | MS.gene70021:CDS | 35.0% | |
| ATTTGATACAAGAAATGGGC+TGG | - | chr3.4:75641607-75641626 | MS.gene70021:CDS | 35.0% | |
| GAAAATTAAACAAGGGAGGT+TGG | - | chr3.4:75642288-75642307 | MS.gene70021:CDS | 35.0% | |
| GAAAGCTTGTAATTTCTTCC+CGG | - | chr3.4:75641512-75641531 | MS.gene70021:intron | 35.0% | |
| GACATAACGGTATTCAACTT+TGG | + | chr3.4:75640986-75641005 | None:intergenic | 35.0% | |
| GACATAGCATGTTTCGTTAA+AGG | - | chr3.4:75641459-75641478 | MS.gene70021:intron | 35.0% | |
| GCAAGAATTTATCTTCCCAA+CGG | - | chr3.4:75641926-75641945 | MS.gene70021:CDS | 35.0% | |
| GCACGATTTGATACAAGAAA+TGG | - | chr3.4:75641602-75641621 | MS.gene70021:CDS | 35.0% | |
| GGAATTGAGGAAAACTATGA+AGG | - | chr3.4:75640721-75640740 | MS.gene70021:CDS | 35.0% | |
| GTACAACCCATTGAATTGAA+GGG | - | chr3.4:75640691-75640710 | MS.gene70021:CDS | 35.0% | |
| TATCGTACCCTTGTTACAAA+TGG | + | chr3.4:75641577-75641596 | None:intergenic | 35.0% | |
| TCTATGGCTAAGAATTTGGA+AGG | - | chr3.4:75642510-75642529 | MS.gene70021:CDS | 35.0% | |
| TTCTTAGCCATAGACAAATC+TGG | + | chr3.4:75642504-75642523 | None:intergenic | 35.0% | |
| ! | CATCTCAGACATTTTTGCAA+AGG | + | chr3.4:75641870-75641889 | None:intergenic | 35.0% |
| !! | AGCTCTTCATATCCACTTTT+AGG | + | chr3.4:75641243-75641262 | None:intergenic | 35.0% |
| !! | CGTTTCTTTGCTGTTAACAT+AGG | + | chr3.4:75642235-75642254 | None:intergenic | 35.0% |
| !!! | CCCAAAACTTTTAAAGCCAA+AGG | + | chr3.4:75641300-75641319 | None:intergenic | 35.0% |
| AATTTATCTTCCCAACGGTC+TGG | - | chr3.4:75641931-75641950 | MS.gene70021:CDS | 40.0% | |
| ACAGCAACCTTCAAAAGCTT+TGG | - | chr3.4:75642059-75642078 | MS.gene70021:CDS | 40.0% | |
| ATGTAAAGCAATTGCTAGGG+TGG | + | chr3.4:75640824-75640843 | None:intergenic | 40.0% | |
| ATTCTCCAAAGAGCTGATTG+AGG | - | chr3.4:75642476-75642495 | MS.gene70021:CDS | 40.0% | |
| CACAACTCCCTTCAATTCAA+TGG | + | chr3.4:75640701-75640720 | None:intergenic | 40.0% | |
| CAGCAACCTTCAAAAGCTTT+GGG | - | chr3.4:75642060-75642079 | MS.gene70021:CDS | 40.0% | |
| CATTGAATTGAAGGGAGTTG+TGG | - | chr3.4:75640699-75640718 | MS.gene70021:CDS | 40.0% | |
| CGTACAACCCATTGAATTGA+AGG | - | chr3.4:75640690-75640709 | MS.gene70021:CDS | 40.0% | |
| CTAATTGACACTAACTACCC+TGG | + | chr3.4:75642269-75642288 | None:intergenic | 40.0% | |
| GATTATGAATGTTTCGGACC+AGG | - | chr3.4:75641087-75641106 | MS.gene70021:intron | 40.0% | |
| GGCATGTAAAGCAATTGCTA+GGG | + | chr3.4:75640827-75640846 | None:intergenic | 40.0% | |
| GTTACAATCACTCTACTTCC+TGG | + | chr3.4:75641108-75641127 | None:intergenic | 40.0% | |
| GTTAGAGTGATTGGGATATG+GGG | - | chr3.4:75640778-75640797 | MS.gene70021:CDS | 40.0% | |
| GTTCAAGAAGTGAAGAAGCA+TGG | - | chr3.4:75641331-75641350 | MS.gene70021:CDS | 40.0% | |
| TATTTGTCAGGGAACTGTAG+CGG | - | chr3.4:75641787-75641806 | MS.gene70021:CDS | 40.0% | |
| TGGCATGTAAAGCAATTGCT+AGG | + | chr3.4:75640828-75640847 | None:intergenic | 40.0% | |
| ! | TGAGGTACCAGATTTGTCTA+TGG | - | chr3.4:75642494-75642513 | MS.gene70021:CDS | 40.0% |
| !! | AACCTTCAAAAGCTTTGGGA+AGG | - | chr3.4:75642064-75642083 | MS.gene70021:CDS | 40.0% |
| !! | AGCTTTTGAAGGTTGCTGTA+AGG | + | chr3.4:75642058-75642077 | None:intergenic | 40.0% |
| !!! | GCTTTTTTCAGAGCTGTTAG+AGG | - | chr3.4:75640936-75640955 | MS.gene70021:intron | 40.0% |
| AAGCTTGTCGAGTGACAGAA+TGG | + | chr3.4:75642580-75642599 | None:intergenic | 45.0% | |
| ACTTCTGCTTCAGGGTCTTT+GGG | + | chr3.4:75641654-75641673 | None:intergenic | 45.0% | |
| AGACAATGATTCCAGACCGT+TGG | + | chr3.4:75641945-75641964 | None:intergenic | 45.0% | |
| AGGTCATCTATTCCAATGCC+CGG | + | chr3.4:75641534-75641553 | None:intergenic | 45.0% | |
| CAGTACATCATACACGTCGT+TGG | + | chr3.4:75641689-75641708 | None:intergenic | 45.0% | |
| CTGAAGCAGAAGTCGATTGT+GGG | - | chr3.4:75641661-75641680 | MS.gene70021:CDS | 45.0% | |
| GACAATGATTCCAGACCGTT+GGG | + | chr3.4:75641944-75641963 | None:intergenic | 45.0% | |
| TGGTACCTCAATCAGCTCTT+TGG | + | chr3.4:75642484-75642503 | None:intergenic | 45.0% | |
| TGTAATTTCTTCCCGGGCAT+TGG | - | chr3.4:75641519-75641538 | MS.gene70021:intron | 45.0% | |
| TTCCATTATGGGACGTGCAA+CGG | - | chr3.4:75641902-75641921 | MS.gene70021:CDS | 45.0% | |
| TTGTCGAGTGACAGAATGGA+AGG | + | chr3.4:75642576-75642595 | None:intergenic | 45.0% | |
| ! | CACAATCGACTTCTGCTTCA+GGG | + | chr3.4:75641662-75641681 | None:intergenic | 45.0% |
| ! | CTCCTTCCCAAAGCTTTTGA+AGG | + | chr3.4:75642069-75642088 | None:intergenic | 45.0% |
| !! | AAAGCTTTGGGAAGGAGTTC+AGG | - | chr3.4:75642072-75642091 | MS.gene70021:CDS | 45.0% |
| AGAAACGGCGTTAACAAGCC+AGG | - | chr3.4:75642248-75642267 | MS.gene70021:CDS | 50.0% | |
| AGTGATTGGGATATGGGGCA+TGG | - | chr3.4:75640783-75640802 | MS.gene70021:CDS | 50.0% | |
| CAAGCTTCTAGACCTCGAAC+TGG | - | chr3.4:75642593-75642612 | MS.gene70021:CDS | 50.0% | |
| CCTGAAGCAGAAGTCGATTG+TGG | - | chr3.4:75641660-75641679 | MS.gene70021:CDS | 50.0% | |
| CGAGTGACAGAATGGAAGGA+TGG | + | chr3.4:75642572-75642591 | None:intergenic | 50.0% | |
| CTTCTAGACCTCGAACTGGA+AGG | - | chr3.4:75642597-75642616 | MS.gene70021:CDS | 50.0% | |
| GAAACGGCGTTAACAAGCCA+GGG | - | chr3.4:75642249-75642268 | MS.gene70021:CDS | 50.0% | |
| GAAGGGAGTTGTGGGAATTG+AGG | - | chr3.4:75640708-75640727 | MS.gene70021:CDS | 50.0% | |
| GACTTCTGCTTCAGGGTCTT+TGG | + | chr3.4:75641655-75641674 | None:intergenic | 50.0% | |
| GGTCATCTATTCCAATGCCC+GGG | + | chr3.4:75641533-75641552 | None:intergenic | 50.0% | |
| GTGATTGGGATATGGGGCAT+GGG | - | chr3.4:75640784-75640803 | MS.gene70021:CDS | 50.0% | |
| TCCATTATGGGACGTGCAAC+GGG | - | chr3.4:75641903-75641922 | MS.gene70021:CDS | 50.0% | |
| TTGCACAACCTTCCAGTTCG+AGG | + | chr3.4:75642608-75642627 | None:intergenic | 50.0% | |
| ! | CCACAATCGACTTCTGCTTC+AGG | + | chr3.4:75641663-75641682 | None:intergenic | 50.0% |
| ATATGGGGCATGGGTGGCAT+AGG | - | chr3.4:75640793-75640812 | MS.gene70021:CDS | 55.0% | |
| ATTGGGATATGGGGCATGGG+TGG | - | chr3.4:75640787-75640806 | MS.gene70021:CDS | 55.0% | |
| CAGGGAACTGTAGCGGTAGA+AGG | - | chr3.4:75641794-75641813 | MS.gene70021:CDS | 55.0% | |
| CCATTATGGGACGTGCAACG+GGG | - | chr3.4:75641904-75641923 | MS.gene70021:CDS | 55.0% | |
| TATGGGGCATGGGTGGCATA+GGG | - | chr3.4:75640794-75640813 | MS.gene70021:CDS | 55.0% | |
| CCCCGTTGCACGTCCCATAA+TGG | + | chr3.4:75641907-75641926 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 75640633 | 75642636 | 75640633 | ID=MS.gene70021 |
| chr3.4 | mRNA | 75640633 | 75642636 | 75640633 | ID=MS.gene70021.t1;Parent=MS.gene70021 |
| chr3.4 | exon | 75641574 | 75642636 | 75641574 | ID=MS.gene70021.t1.exon1;Parent=MS.gene70021.t1 |
| chr3.4 | CDS | 75641574 | 75642636 | 75641574 | ID=cds.MS.gene70021.t1;Parent=MS.gene70021.t1 |
| chr3.4 | exon | 75641176 | 75641472 | 75641176 | ID=MS.gene70021.t1.exon2;Parent=MS.gene70021.t1 |
| chr3.4 | CDS | 75641176 | 75641472 | 75641176 | ID=cds.MS.gene70021.t1;Parent=MS.gene70021.t1 |
| chr3.4 | exon | 75640633 | 75640825 | 75640633 | ID=MS.gene70021.t1.exon3;Parent=MS.gene70021.t1 |
| chr3.4 | CDS | 75640633 | 75640825 | 75640633 | ID=cds.MS.gene70021.t1;Parent=MS.gene70021.t1 |
| Gene Sequence |
| Protein sequence |
