AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84994


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84994.t1 MTR_7g062720 65.152 132 46 0 1 132 1 132 1.42e-60 190
MS.gene84994.t1 MTR_5g061150 62.992 127 47 0 20 146 10 136 5.12e-50 162
MS.gene84994.t1 MTR_1g036690 58.537 82 27 3 205 285 5 80 1.87e-23 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGCTCTTAATATTTC+TGG 0.080746 5.2:+17690221 MS.gene84994:CDS
TATTGTTGGGGTTCTATTTC+TGG 0.109431 5.2:-17691276 None:intergenic
TTCGGCATTTAAATGAATTT+TGG 0.166319 5.2:-17690273 None:intergenic
ATGGGTACATTTACGTTTAC+AGG 0.235783 5.2:+17690496 MS.gene84994:CDS
CCAACAATCACTTCAAAATA+AGG 0.249520 5.2:+17691114 MS.gene84994:CDS
AAATTGTCCCAAAGATCTTT+TGG 0.267031 5.2:-17690419 None:intergenic
TATTTCTGGAAATAATTATC+TGG 0.282933 5.2:+17690235 MS.gene84994:CDS
TAGAAGATCCAAAAGATCTT+TGG 0.283634 5.2:+17690411 MS.gene84994:CDS
TAAAGATCAACAAGATGTTT+TGG 0.343855 5.2:-17691091 None:intergenic
CTGGAAATAATTATCTGGCT+TGG 0.347827 5.2:+17690240 MS.gene84994:CDS
TGGAAATAATTATCTGGCTT+GGG 0.361577 5.2:+17690241 MS.gene84994:CDS
TGAAGATGATTATGGTAATA+TGG 0.367220 5.2:+17691174 MS.gene84994:CDS
GAAAATGATGAAGATGATTA+TGG 0.394855 5.2:+17691166 MS.gene84994:CDS
TTTGGGACAATTTAAAAGAA+AGG 0.395342 5.2:+17690429 MS.gene84994:CDS
AGAAGATCCAAAAGATCTTT+GGG 0.400056 5.2:+17690412 MS.gene84994:CDS
ACCCATTCGTTACGAGCATT+AGG 0.408672 5.2:-17690479 None:intergenic
AGATGATCAATCATTCCTTC+AGG 0.429528 5.2:-17691235 None:intergenic
CCTTCGTCACCACCTTCATA+AGG 0.433246 5.2:+17690364 MS.gene84994:CDS
CACTTCAAAATAAGGACAAA+AGG 0.441641 5.2:+17691122 MS.gene84994:CDS
GTTGTGGAGGCAAAGGTCAT+TGG 0.462627 5.2:+17691050 MS.gene84994:CDS
TATAGTATCTCCATGCCCTT+CGG 0.465237 5.2:-17690291 None:intergenic
TGAAACAACTGAACAACAAA+AGG 0.469111 5.2:+17690325 MS.gene84994:CDS
AATGTTGTAAAGAATGATAT+TGG 0.471241 5.2:+17690992 MS.gene84994:CDS
TTTCGCGGTATTGTTGCTGC+AGG 0.473557 5.2:-17690652 None:intergenic
ATTGACACTGTGTGGAGAAA+AGG 0.483584 5.2:+17690571 MS.gene84994:CDS
TCCTAATGCTCGTAACGAAT+GGG 0.483778 5.2:+17690478 MS.gene84994:CDS
CATTTAAATGCCGAAGGGCA+TGG 0.494088 5.2:+17690281 MS.gene84994:CDS
AGAAATCAGCAGCATCTAAG+TGG 0.497006 5.2:-17691204 None:intergenic
TTCCTAATGCTCGTAACGAA+TGG 0.505183 5.2:+17690477 MS.gene84994:CDS
AACTGAACAACAAAAGGTTA+AGG 0.508959 5.2:+17690331 MS.gene84994:CDS
TACATTAGATGCATGAAAAG+TGG 0.516304 5.2:-17690627 None:intergenic
GAGAGTATTTGTTATCGTTG+TGG 0.516470 5.2:+17691034 MS.gene84994:CDS
CATGGAGATACTATAAAAGA+AGG 0.519449 5.2:+17690299 MS.gene84994:CDS
GATTTCTTTGCTGATCCTGA+AGG 0.528813 5.2:+17691220 MS.gene84994:CDS
ACTTCAAAATAAGGACAAAA+GGG 0.529166 5.2:+17691123 MS.gene84994:CDS
TGTTATCGTTGTGGAGGCAA+AGG 0.533037 5.2:+17691043 MS.gene84994:CDS
AAAATTCATTTAAATGCCGA+AGG 0.537172 5.2:+17690275 MS.gene84994:CDS
GGAATGATTGATCATCTCAC+TGG 0.540490 5.2:+17691241 MS.gene84994:CDS
TGCAGCAACAATACCGCGAA+AGG 0.561209 5.2:+17690654 MS.gene84994:CDS
GATGATTATGGTAATATGGA+AGG 0.564748 5.2:+17691178 MS.gene84994:CDS
AGTATTTGTTATCGTTGTGG+AGG 0.570678 5.2:+17691037 MS.gene84994:CDS
GTCTGTAATCGAATATAACT+CGG 0.574961 5.2:+17690526 MS.gene84994:CDS
ATTCGTTACGAGCATTAGGA+AGG 0.589689 5.2:-17690475 None:intergenic
CCTTATGAAGGTGGTGACGA+AGG 0.598722 5.2:-17690364 None:intergenic
GAAATCAGCAGCATCTAAGT+GGG 0.621669 5.2:-17691203 None:intergenic
AAATTCATTTAAATGCCGAA+GGG 0.631745 5.2:+17690276 MS.gene84994:CDS
GTGGTGACGAAGGAATATCA+TGG 0.663314 5.2:-17690354 None:intergenic
ACTTCTCTATTGACACTGTG+TGG 0.668278 5.2:+17690563 MS.gene84994:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTGTAAAGAATGATAT+TGG + chr5.2:17690992-17691011 MS.gene84994:CDS 20.0%
!!! TATTTCTGGAAATAATTATC+TGG + chr5.2:17690235-17690254 MS.gene84994:CDS 20.0%
! AAAATTCATTTAAATGCCGA+AGG + chr5.2:17690275-17690294 MS.gene84994:CDS 25.0%
! AAATTCATTTAAATGCCGAA+GGG + chr5.2:17690276-17690295 MS.gene84994:CDS 25.0%
! ACTTCAAAATAAGGACAAAA+GGG + chr5.2:17691123-17691142 MS.gene84994:CDS 25.0%
! GAAAATGATGAAGATGATTA+TGG + chr5.2:17691166-17691185 MS.gene84994:CDS 25.0%
! TGAAGATGATTATGGTAATA+TGG + chr5.2:17691174-17691193 MS.gene84994:CDS 25.0%
!! TAAAGATCAACAAGATGTTT+TGG - chr5.2:17691094-17691113 None:intergenic 25.0%
!! TTACAACATTTTTCATCTTC+TGG - chr5.2:17690982-17691001 None:intergenic 25.0%
!! TTCGGCATTTAAATGAATTT+TGG - chr5.2:17690276-17690295 None:intergenic 25.0%
!! TTTGGGACAATTTAAAAGAA+AGG + chr5.2:17690429-17690448 MS.gene84994:CDS 25.0%
!!! CATTTTTTAGATCCTTATGA+AGG - chr5.2:17690379-17690398 None:intergenic 25.0%
!!! GAGAAAATGTGTTTTTGTTT+TGG - chr5.2:17690958-17690977 None:intergenic 25.0%
!!! GGTTTTAATAGTAATCACAA+TGG + chr5.2:17690908-17690927 MS.gene84994:intron 25.0%
!!! TAAATGAATTTTGGCATCAA+GGG - chr5.2:17690267-17690286 None:intergenic 25.0%
!!! TTAAATGAATTTTGGCATCA+AGG - chr5.2:17690268-17690287 None:intergenic 25.0%
AACTGAACAACAAAAGGTTA+AGG + chr5.2:17690331-17690350 MS.gene84994:CDS 30.0%
AGAAGATCCAAAAGATCTTT+GGG + chr5.2:17690412-17690431 MS.gene84994:CDS 30.0%
CACTTCAAAATAAGGACAAA+AGG + chr5.2:17691122-17691141 MS.gene84994:CDS 30.0%
CATGGAGATACTATAAAAGA+AGG + chr5.2:17690299-17690318 MS.gene84994:CDS 30.0%
CCAACAATCACTTCAAAATA+AGG + chr5.2:17691114-17691133 MS.gene84994:CDS 30.0%
GATGATTATGGTAATATGGA+AGG + chr5.2:17691178-17691197 MS.gene84994:CDS 30.0%
GTCTGTAATCGAATATAACT+CGG + chr5.2:17690526-17690545 MS.gene84994:CDS 30.0%
TACATTAGATGCATGAAAAG+TGG - chr5.2:17690630-17690649 None:intergenic 30.0%
TAGAAGATCCAAAAGATCTT+TGG + chr5.2:17690411-17690430 MS.gene84994:CDS 30.0%
TGAAACAACTGAACAACAAA+AGG + chr5.2:17690325-17690344 MS.gene84994:CDS 30.0%
! AAATTGTCCCAAAGATCTTT+TGG - chr5.2:17690422-17690441 None:intergenic 30.0%
! AAGATCTTTTGGATCTTCTA+TGG - chr5.2:17690411-17690430 None:intergenic 30.0%
! TGGAAATAATTATCTGGCTT+GGG + chr5.2:17690241-17690260 MS.gene84994:CDS 30.0%
! TTTGAGGCTCTTAATATTTC+TGG + chr5.2:17690221-17690240 MS.gene84994:CDS 30.0%
! TTTTTAGATCCTTATGAAGG+TGG - chr5.2:17690376-17690395 None:intergenic 30.0%
!!! CCTTATTTTGAAGTGATTGT+TGG - chr5.2:17691117-17691136 None:intergenic 30.0%
!!! TATTTTGTTTTGCCAGAAGT+AGG - chr5.2:17690712-17690731 None:intergenic 30.0%
AGATGATCAATCATTCCTTC+AGG - chr5.2:17691238-17691257 None:intergenic 35.0%
ATGGGTACATTTACGTTTAC+AGG + chr5.2:17690496-17690515 MS.gene84994:CDS 35.0%
CTGGAAATAATTATCTGGCT+TGG + chr5.2:17690240-17690259 MS.gene84994:CDS 35.0%
CTTAATATCATGCCTACTTC+TGG + chr5.2:17690697-17690716 MS.gene84994:intron 35.0%
! CCTCAAAATCAAGTTTTGCA+AGG - chr5.2:17690208-17690227 None:intergenic 35.0%
! CCTTGCAAAACTTGATTTTG+AGG + chr5.2:17690205-17690224 MS.gene84994:CDS 35.0%
! CTTTTGGATCTTCTATGGTA+AGG - chr5.2:17690406-17690425 None:intergenic 35.0%
!! AGTATTTGTTATCGTTGTGG+AGG + chr5.2:17691037-17691056 MS.gene84994:CDS 35.0%
!! GAGAGTATTTGTTATCGTTG+TGG + chr5.2:17691034-17691053 MS.gene84994:CDS 35.0%
!!! ACCTTTTAAAACTCCTTTCG+CGG - chr5.2:17690670-17690689 None:intergenic 35.0%
ACGTGGTCGTGGTTATTTAA+GGG + chr5.2:17690874-17690893 MS.gene84994:intron 40.0%
ACTTCTCTATTGACACTGTG+TGG + chr5.2:17690563-17690582 MS.gene84994:CDS 40.0%
AGAAATCAGCAGCATCTAAG+TGG - chr5.2:17691207-17691226 None:intergenic 40.0%
ATTCGTTACGAGCATTAGGA+AGG - chr5.2:17690478-17690497 None:intergenic 40.0%
ATTGACACTGTGTGGAGAAA+AGG + chr5.2:17690571-17690590 MS.gene84994:CDS 40.0%
GAAATCAGCAGCATCTAAGT+GGG - chr5.2:17691206-17691225 None:intergenic 40.0%
GATTTCTTTGCTGATCCTGA+AGG + chr5.2:17691220-17691239 MS.gene84994:CDS 40.0%
GCGTTGCTACATTCACTTAT+GGG - chr5.2:17690787-17690806 None:intergenic 40.0%
GCTACATTCACTTATGGGAA+TGG - chr5.2:17690782-17690801 None:intergenic 40.0%
GGAATGATTGATCATCTCAC+TGG + chr5.2:17691241-17691260 MS.gene84994:CDS 40.0%
TATAGTATCTCCATGCCCTT+CGG - chr5.2:17690294-17690313 None:intergenic 40.0%
TCCTAATGCTCGTAACGAAT+GGG + chr5.2:17690478-17690497 MS.gene84994:CDS 40.0%
TGCGTTGCTACATTCACTTA+TGG - chr5.2:17690788-17690807 None:intergenic 40.0%
TTCCTAATGCTCGTAACGAA+TGG + chr5.2:17690477-17690496 MS.gene84994:CDS 40.0%
! GAAGGATGACAGTTTTCTGA+TGG - chr5.2:17690460-17690479 None:intergenic 40.0%
! TATTTAAGGGGACGTGGTTT+TGG + chr5.2:17690887-17690906 MS.gene84994:intron 40.0%
!!! AAGATGTTTTGGTGTACGAC+AGG - chr5.2:17691083-17691102 None:intergenic 40.0%
!!! ACCGCGAAAGGAGTTTTAAA+AGG + chr5.2:17690666-17690685 MS.gene84994:CDS 40.0%
ACCCATTCGTTACGAGCATT+AGG - chr5.2:17690482-17690501 None:intergenic 45.0%
CATTTAAATGCCGAAGGGCA+TGG + chr5.2:17690281-17690300 MS.gene84994:CDS 45.0%
CGTGGTCGTGGTTATTTAAG+GGG + chr5.2:17690875-17690894 MS.gene84994:intron 45.0%
GACGTGGTCGTGGTTATTTA+AGG + chr5.2:17690873-17690892 MS.gene84994:intron 45.0%
GATCACTATGGACAAACTCG+TGG + chr5.2:17690809-17690828 MS.gene84994:intron 45.0%
GTAGCAACGCATGATCACTA+TGG + chr5.2:17690797-17690816 MS.gene84994:intron 45.0%
TGTTATCGTTGTGGAGGCAA+AGG + chr5.2:17691043-17691062 MS.gene84994:CDS 45.0%
! GTGGTGACGAAGGAATATCA+TGG - chr5.2:17690357-17690376 None:intergenic 45.0%
CCTTCGTCACCACCTTCATA+AGG + chr5.2:17690364-17690383 MS.gene84994:CDS 50.0%
CGTGGTTATTTAAGGGGACG+TGG + chr5.2:17690881-17690900 MS.gene84994:intron 50.0%
GTTGTGGAGGCAAAGGTCAT+TGG + chr5.2:17691050-17691069 MS.gene84994:CDS 50.0%
TATGGACAAACTCGTGGTCG+TGG + chr5.2:17690815-17690834 MS.gene84994:intron 50.0%
TGCAGCAACAATACCGCGAA+AGG + chr5.2:17690654-17690673 MS.gene84994:CDS 50.0%
TTTCGCGGTATTGTTGCTGC+AGG - chr5.2:17690655-17690674 None:intergenic 50.0%
! CCTTATGAAGGTGGTGACGA+AGG - chr5.2:17690367-17690386 None:intergenic 50.0%
AATCATGCAGCTCGTCCCAC+TGG + chr5.2:17690749-17690768 MS.gene84994:intron 55.0%
CAAACTCGTGGTCGTGGTTG+TGG + chr5.2:17690821-17690840 MS.gene84994:intron 55.0%
CACGTCCACGACCATAACCA+TGG - chr5.2:17690847-17690866 None:intergenic 55.0%
CATGGTTATGGTCGTGGACG+TGG + chr5.2:17690845-17690864 MS.gene84994:intron 55.0%
CGTGGTTGTGGCCATGGTTA+TGG + chr5.2:17690833-17690852 MS.gene84994:intron 55.0%
TGTGGCCATGGTTATGGTCG+TGG + chr5.2:17690839-17690858 MS.gene84994:intron 55.0%
! GGGAATGGAGCACTACCAGT+GGG - chr5.2:17690767-17690786 None:intergenic 55.0%
! TGGGAATGGAGCACTACCAG+TGG - chr5.2:17690768-17690787 None:intergenic 55.0%
TATGGTCGTGGACGTGGACG+TGG + chr5.2:17690851-17690870 MS.gene84994:intron 60.0%
CGTGGTCGTGGTTGTGGCCA+TGG + chr5.2:17690827-17690846 MS.gene84994:intron 65.0%
CGTGGACGTGGACGTGGACG+TGG + chr5.2:17690857-17690876 MS.gene84994:intron 70.0%
CGTGGACGTGGACGTGGTCG+TGG + chr5.2:17690863-17690882 MS.gene84994:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 17690197 17691285 17690197 ID=MS.gene84994
chr5.2 mRNA 17690197 17691285 17690197 ID=MS.gene84994.t1;Parent=MS.gene84994
chr5.2 exon 17690197 17690687 17690197 ID=MS.gene84994.t1.exon1;Parent=MS.gene84994.t1
chr5.2 CDS 17690197 17690687 17690197 ID=cds.MS.gene84994.t1;Parent=MS.gene84994.t1
chr5.2 exon 17690919 17691285 17690919 ID=MS.gene84994.t1.exon2;Parent=MS.gene84994.t1
chr5.2 CDS 17690919 17691285 17690919 ID=cds.MS.gene84994.t1;Parent=MS.gene84994.t1
Gene Sequence

>MS.gene84994

ATGTCGAACCTTGCAAAACTTGATTTTGAGGCTCTTAATATTTCTGGAAATAATTATCTGGCTTGGGCCCTTGATGCCAAAATTCATTTAAATGCCGAAGGGCATGGAGATACTATAAAAGAAGGAAATGAAACAACTGAACAACAAAAGGTTAAGGCCATGATATTCCTTCGTCACCACCTTCATAAGGATCTAAAAAATGAATACCTTACCATAGAAGATCCAAAAGATCTTTGGGACAATTTAAAAGAAAGGTTTGACCATCAGAAAACTGTCATCCTTCCTAATGCTCGTAACGAATGGGTACATTTACGTTTACAGGACTTTAAGTCTGTAATCGAATATAACTCGGCTGTATTTAAGATTACTTCTCTATTGACACTGTGTGGAGAAAAGGTGACTGATGCGCAGATGTTAGAAAAAACATTCTCCACTTTTCATGCATCTAATGTAATCCTGCAGCAACAATACCGCGAAAGGAGTTTTAAAAGGTATTGTGACTTAATATCATGCCTACTTCTGGCAAAACAAAATAACGAGCTTTTGATGAAAAATCATGCAGCTCGTCCCACTGGTAGTGCTCCATTCCCATAAGTGAATGTAGCAACGCATGATCACTATGGACAAACTCGTGGTCGTGGTTGTGGCCATGGTTATGGTCGTGGACGTGGACGTGGACGTGGTCGTGGTTATTTAAGGGGACGTGGTTTTGGTTTTAATAGTAATCACAATGGAGATTATAAGAACACATACTCTCACCAAAACAAAAACACATTTTCTCACCAGAAGATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGTTATCGTTGTGGAGGCAAAGGTCATTGGAGTCGTACCTGTCGTACACCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGACAAAAGGGCTGAAACGCATTTTGCATGTGAAAATGATGAAGATGATTATGGTAATATGGAAGGTACCCACTTAGATGCTGCTGATTTCTTTGCTGATCCTGAAGGAATGATTGATCATCTCACTGGAGATGAAAATGTCCAGAAATAG

Protein sequence

>MS.gene84994.t1

MSNLAKLDFEALNISGNNYLAWALDAKIHLNAEGHGDTIKEGNETTEQQKVKAMIFLRHHLHKDLKNEYLTIEDPKDLWDNLKERFDHQKTVILPNARNEWVHLRLQDFKSVIEYNSAVFKITSLLTLCGEKVTDAQMLEKTFSTFHASNVILQQQYRERSFKSNHNGDYKNTYSHQNKNTFSHQKMKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMEGTHLDAADFFADPEGMIDHLTGDENVQK