Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061658.t1 | XP_013466683.1 | 97 | 267 | 8 | 0 | 1 | 267 | 1 | 267 | 3.30E-140 | 507.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061658.t1 | Q9ZUM2 | 81.0 | 252 | 48 | 0 | 16 | 267 | 22 | 273 | 2.2e-116 | 419.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061658.t1 | A0A072VRG4 | 97.0 | 267 | 8 | 0 | 1 | 267 | 1 | 267 | 2.4e-140 | 507.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene061658 | -0.802258 | 6.11E-49 | -1.69E-46 |
| MS.gene049297 | MS.gene061658 | -0.83315 | 6.29E-56 | -1.69E-46 |
| MS.gene049378 | MS.gene061658 | -0.834281 | 3.28E-56 | -1.69E-46 |
| MS.gene049669 | MS.gene061658 | -0.819374 | 1.20E-52 | -1.69E-46 |
| MS.gene050226 | MS.gene061658 | -0.809185 | 2.14E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene061658 | -0.839582 | 1.45E-57 | -1.69E-46 |
| MS.gene050228 | MS.gene061658 | -0.839802 | 1.27E-57 | -1.69E-46 |
| MS.gene050303 | MS.gene061658 | -0.814441 | 1.54E-51 | -1.69E-46 |
| MS.gene050330 | MS.gene061658 | 0.859306 | 4.53E-63 | -1.69E-46 |
| MS.gene050442 | MS.gene061658 | -0.812879 | 3.40E-51 | -1.69E-46 |
| MS.gene050558 | MS.gene061658 | -0.828106 | 1.08E-54 | -1.69E-46 |
| MS.gene050623 | MS.gene061658 | 0.852597 | 4.16E-61 | -1.69E-46 |
| MS.gene050642 | MS.gene061658 | -0.849408 | 3.30E-60 | -1.69E-46 |
| MS.gene050719 | MS.gene061658 | -0.811986 | 5.32E-51 | -1.69E-46 |
| MS.gene050721 | MS.gene061658 | -0.813105 | 3.03E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene061658 | -0.811623 | 6.38E-51 | -1.69E-46 |
| MS.gene050787 | MS.gene061658 | -0.820323 | 7.29E-53 | -1.69E-46 |
| MS.gene050820 | MS.gene061658 | -0.822585 | 2.19E-53 | -1.69E-46 |
| MS.gene050953 | MS.gene061658 | -0.808154 | 3.56E-50 | -1.69E-46 |
| MS.gene051207 | MS.gene061658 | -0.805234 | 1.47E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene061658 | -0.816929 | 4.29E-52 | -1.69E-46 |
| MS.gene051983 | MS.gene061658 | -0.838678 | 2.49E-57 | -1.69E-46 |
| MS.gene052077 | MS.gene061658 | -0.807513 | 4.87E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene061658 | -0.870403 | 1.50E-66 | -1.69E-46 |
| MS.gene052190 | MS.gene061658 | -0.812696 | 3.72E-51 | -1.69E-46 |
| MS.gene052309 | MS.gene061658 | 0.872294 | 3.55E-67 | -1.69E-46 |
| MS.gene052358 | MS.gene061658 | 0.861592 | 9.21E-64 | -1.69E-46 |
| MS.gene052361 | MS.gene061658 | 0.814189 | 1.75E-51 | -1.69E-46 |
| MS.gene052376 | MS.gene061658 | -0.809495 | 1.84E-50 | -1.69E-46 |
| MS.gene053054 | MS.gene061658 | 0.812692 | 3.73E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene061658 | -0.831352 | 1.75E-55 | -1.69E-46 |
| MS.gene054291 | MS.gene061658 | 0.811006 | 8.69E-51 | -1.69E-46 |
| MS.gene054405 | MS.gene061658 | -0.804807 | 1.81E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene061658 | -0.810236 | 1.27E-50 | -1.69E-46 |
| MS.gene054473 | MS.gene061658 | -0.809019 | 2.33E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene061658 | -0.807104 | 5.95E-50 | -1.69E-46 |
| MS.gene054942 | MS.gene061658 | -0.824356 | 8.44E-54 | -1.69E-46 |
| MS.gene054983 | MS.gene061658 | -0.833042 | 6.69E-56 | -1.69E-46 |
| MS.gene055356 | MS.gene061658 | -0.813404 | 2.60E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene061658 | -0.818944 | 1.50E-52 | -1.69E-46 |
| MS.gene056069 | MS.gene061658 | 0.822641 | 2.13E-53 | -1.69E-46 |
| MS.gene056087 | MS.gene061658 | -0.82601 | 3.43E-54 | -1.69E-46 |
| MS.gene056506 | MS.gene061658 | -0.82454 | 7.64E-54 | -1.69E-46 |
| MS.gene056979 | MS.gene061658 | -0.823326 | 1.47E-53 | -1.69E-46 |
| MS.gene057260 | MS.gene061658 | -0.811337 | 7.36E-51 | -1.69E-46 |
| MS.gene057967 | MS.gene061658 | -0.803529 | 3.34E-49 | -1.69E-46 |
| MS.gene058200 | MS.gene061658 | -0.809093 | 2.24E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene061658 | -0.825687 | 4.09E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene061658 | -0.801125 | 1.04E-48 | -1.69E-46 |
| MS.gene060137 | MS.gene061658 | 0.812311 | 4.52E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene061658 | -0.808793 | 2.60E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene061658 | -0.811116 | 8.22E-51 | -1.69E-46 |
| MS.gene060683 | MS.gene061658 | -0.806161 | 9.41E-50 | -1.69E-46 |
| MS.gene060685 | MS.gene061658 | -0.804027 | 2.63E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene061658 | -0.809924 | 1.49E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene061658 | 0.963205 | 8.43E-122 | -1.69E-46 |
| MS.gene061114 | MS.gene061658 | 0.854175 | 1.47E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061658.t1 | MTR_1g033830 | 97.004 | 267 | 8 | 0 | 1 | 267 | 1 | 267 | 0.0 | 528 |
| MS.gene061658.t1 | MTR_1g033830 | 96.413 | 223 | 8 | 0 | 1 | 223 | 1 | 223 | 1.10e-156 | 435 |
| MS.gene061658.t1 | MTR_4g094212 | 55.466 | 247 | 110 | 0 | 21 | 267 | 13 | 259 | 9.15e-102 | 298 |
| MS.gene061658.t1 | MTR_2g022880 | 56.962 | 237 | 97 | 1 | 31 | 267 | 23 | 254 | 1.28e-94 | 280 |
| MS.gene061658.t1 | MTR_4g131930 | 55.285 | 246 | 110 | 0 | 22 | 267 | 3 | 248 | 8.67e-90 | 267 |
| MS.gene061658.t1 | MTR_2g022880 | 56.744 | 215 | 88 | 1 | 31 | 245 | 23 | 232 | 1.52e-82 | 248 |
| MS.gene061658.t1 | MTR_4g131930 | 54.595 | 185 | 84 | 0 | 83 | 267 | 1 | 185 | 6.01e-63 | 197 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061658.t1 | AT2G02180 | 81.275 | 251 | 47 | 0 | 17 | 267 | 23 | 273 | 2.76e-155 | 434 |
| MS.gene061658.t1 | AT1G14530 | 78.968 | 252 | 53 | 0 | 16 | 267 | 12 | 263 | 7.81e-143 | 402 |
| MS.gene061658.t1 | AT1G14530 | 78.855 | 227 | 48 | 0 | 16 | 242 | 12 | 238 | 4.23e-135 | 381 |
| MS.gene061658.t1 | AT4G21790 | 57.200 | 250 | 107 | 0 | 18 | 267 | 12 | 261 | 3.07e-105 | 307 |
Find 67 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCACTTCTGGTCTTGTTC+TGG | 0.161658 | 1.3:+18721342 | MS.gene061658:CDS |
| CTGTCTAAGGTGTTCTTTGC+AGG | 0.219822 | 1.3:+18724236 | MS.gene061658:CDS |
| CTTTACAACATATGCACTTC+TGG | 0.271976 | 1.3:+18721331 | MS.gene061658:CDS |
| TGGGTAGTCCAACCATATTC+TGG | 0.305600 | 1.3:-18720030 | None:intergenic |
| TGAGAAAATGAAACACCTTC+TGG | 0.307420 | 1.3:-18720050 | None:intergenic |
| CAATTGAGAGTTCCAGAATA+TGG | 0.308572 | 1.3:+18720018 | MS.gene061658:CDS |
| TGTTGTGGTTTATGCTGTTC+AGG | 0.311273 | 1.3:+18721549 | MS.gene061658:CDS |
| TGCACTTCTGGTCTTGTTCT+GGG | 0.328875 | 1.3:+18721343 | MS.gene061658:CDS |
| TACACCCTCGCCGGCCTTTA+CGG | 0.339761 | 1.3:+18719816 | MS.gene061658:CDS |
| TTTCCTTATCAGAAGTGTTA+TGG | 0.345609 | 1.3:+18726393 | MS.gene061658:CDS |
| CGTACTAGTCATTCTGTCTA+AGG | 0.350388 | 1.3:+18724223 | MS.gene061658:CDS |
| CAGCTTCTTACGACGCCCTT+TGG | 0.359651 | 1.3:-18724951 | None:intergenic |
| GTTTCTTTGTTTGCTGCCCT+TGG | 0.365159 | 1.3:+18724358 | MS.gene061658:CDS |
| CGACGCCCTTTGGATTCAAC+AGG | 0.380572 | 1.3:-18724941 | None:intergenic |
| TAGGATGGTCCATCACATTA+AGG | 0.382104 | 1.3:-18726530 | None:intergenic |
| CTGGGCTGAGATTTATTACC+AGG | 0.402191 | 1.3:+18721361 | MS.gene061658:CDS |
| CGTCGTTTCCGCCATAGCTT+TGG | 0.423002 | 1.3:+18719839 | MS.gene061658:CDS |
| GCCCTTGGCTTTCTTCTCTA+CGG | 0.437396 | 1.3:+18724373 | MS.gene061658:CDS |
| GAGAAAATGAAACACCTTCT+GGG | 0.439545 | 1.3:-18720049 | None:intergenic |
| GAACGACATCGACGATTCTC+CGG | 0.444368 | 1.3:+18719773 | MS.gene061658:CDS |
| AAGATGCGGTCTTGCCAAAC+CGG | 0.453837 | 1.3:-18719792 | None:intergenic |
| ACCTCGCCGTCGTCGGTGAC+AGG | 0.458409 | 1.3:+18719696 | MS.gene061658:CDS |
| GTTCCACCAGGTTGAGGAAT+CGG | 0.468815 | 1.3:-18719754 | None:intergenic |
| CGCTTTCCTGTTGAATCCAA+AGG | 0.469858 | 1.3:+18724935 | MS.gene061658:CDS |
| ACATTAATTGTATAGAAACT+TGG | 0.472412 | 1.3:-18721529 | None:intergenic |
| TCACCATAACACTTCTGATA+AGG | 0.473201 | 1.3:-18726396 | None:intergenic |
| CGACGACGGCGAGGTGTTGT+TGG | 0.475128 | 1.3:-18719688 | None:intergenic |
| ACATCGACGATTCTCCGGTT+TGG | 0.476060 | 1.3:+18719778 | MS.gene061658:CDS |
| GCACATCAAGTAAGATATGT+TGG | 0.497834 | 1.3:-18721295 | None:intergenic |
| GCTTTCTTCTCTACGGTGGA+AGG | 0.504569 | 1.3:+18724380 | MS.gene061658:CDS |
| TAATATATGAAGTTTAGAAT+AGG | 0.508408 | 1.3:-18726549 | None:intergenic |
| TTTCTATACAATTAATGTTG+TGG | 0.510519 | 1.3:+18721534 | MS.gene061658:CDS |
| TGGCGGAAACGACGCCGTAA+AGG | 0.516515 | 1.3:-18719830 | None:intergenic |
| TGAGAGTTCCAGAATATGGT+TGG | 0.518623 | 1.3:+18720022 | MS.gene061658:CDS |
| ATCGTCGATGTCGTTCCACC+AGG | 0.520262 | 1.3:-18719766 | None:intergenic |
| GGAAACGACGCCGTAAAGGC+CGG | 0.530370 | 1.3:-18719826 | None:intergenic |
| GCTTTCCTGTTGAATCCAAA+GGG | 0.544156 | 1.3:+18724936 | MS.gene061658:CDS |
| GCACGTGCTGTATCCACTGA+TGG | 0.558795 | 1.3:+18721499 | MS.gene061658:CDS |
| AAACGCGAACCTTAATGTGA+TGG | 0.562294 | 1.3:+18726521 | MS.gene061658:CDS |
| GATGTCGTTCCACCAGGTTG+AGG | 0.564379 | 1.3:-18719760 | None:intergenic |
| ATGGGAAGGTACCAAAGCTA+TGG | 0.578903 | 1.3:-18719850 | None:intergenic |
| CGACGCCGTAAAGGCCGGCG+AGG | 0.579591 | 1.3:-18719821 | None:intergenic |
| GACAGGCGCGGTGGTGAACG+CGG | 0.588632 | 1.3:+18719713 | MS.gene061658:CDS |
| TCTAAACTTCATATATTACC+TGG | 0.590927 | 1.3:+18726554 | MS.gene061658:CDS |
| AACTCACCGATTCCTCAACC+TGG | 0.591877 | 1.3:+18719748 | MS.gene061658:CDS |
| CTTGGCTTTCTTCTCTACGG+TGG | 0.595848 | 1.3:+18724376 | MS.gene061658:CDS |
| ACCGTAGAGAAGAAAGCCAA+GGG | 0.603119 | 1.3:-18724374 | None:intergenic |
| CAACAACACCTCGCCGTCGT+CGG | 0.609317 | 1.3:+18719689 | MS.gene061658:CDS |
| GCCGTCGTCGGTGACAGGCG+CGG | 0.609384 | 1.3:+18719701 | MS.gene061658:CDS |
| GACGCCGTAAAGGCCGGCGA+GGG | 0.609478 | 1.3:-18719820 | None:intergenic |
| CACCGTAGAGAAGAAAGCCA+AGG | 0.609487 | 1.3:-18724375 | None:intergenic |
| ACTTGGCTTCAGTCCATCAG+TGG | 0.611275 | 1.3:-18721512 | None:intergenic |
| GTTGTAAAGAAAGCAAGACT+TGG | 0.617097 | 1.3:-18721317 | None:intergenic |
| AGAATGACTAGTACGCTGAC+TGG | 0.622590 | 1.3:-18724215 | None:intergenic |
| ACCGCGCCTGTCACCGACGA+CGG | 0.629626 | 1.3:-18719702 | None:intergenic |
| CGCATCTTCTACACCCTCGC+CGG | 0.632929 | 1.3:+18719807 | MS.gene061658:CDS |
| GGAAGGTACCAAAGCTATGG+CGG | 0.644462 | 1.3:-18719847 | None:intergenic |
| ATATGAAGTTTAGAATAGGA+TGG | 0.649295 | 1.3:-18726545 | None:intergenic |
| GCCTGTCACCGACGACGGCG+AGG | 0.655892 | 1.3:-18719697 | None:intergenic |
| CGGCGAGGGTGTAGAAGATG+CGG | 0.656168 | 1.3:-18719806 | None:intergenic |
| ATATGGTTGGACTACCCAGA+AGG | 0.660843 | 1.3:+18720035 | MS.gene061658:CDS |
| TCTTCTTTCGGAATGTACAG+AGG | 0.665668 | 1.3:+18720232 | MS.gene061658:CDS |
| GCGTCGTAAGAAGCTGCAAG+AGG | 0.671751 | 1.3:+18724958 | MS.gene061658:CDS |
| GTCGTCGGTGACAGGCGCGG+TGG | 0.677193 | 1.3:+18719704 | MS.gene061658:CDS |
| TGTACAGAGGTTACAGCCAG+AGG | 0.679431 | 1.3:+18720245 | MS.gene061658:CDS |
| AGGCGCGGTGGTGAACGCGG+TGG | 0.685538 | 1.3:+18719716 | MS.gene061658:CDS |
| TCACCGATTCCTCAACCTGG+TGG | 0.693137 | 1.3:+18719751 | MS.gene061658:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 18719684 | 18726575 | 18719684 | ID=MS.gene061658 |
| chr1.3 | mRNA | 18719684 | 18726575 | 18719684 | ID=MS.gene061658.t1;Parent=MS.gene061658 |
| chr1.3 | exon | 18719684 | 18719860 | 18719684 | ID=MS.gene061658.t1.exon1;Parent=MS.gene061658.t1 |
| chr1.3 | CDS | 18719684 | 18719860 | 18719684 | ID=cds.MS.gene061658.t1;Parent=MS.gene061658.t1 |
| chr1.3 | exon | 18720000 | 18720090 | 18720000 | ID=MS.gene061658.t1.exon2;Parent=MS.gene061658.t1 |
| chr1.3 | CDS | 18720000 | 18720090 | 18720000 | ID=cds.MS.gene061658.t1;Parent=MS.gene061658.t1 |
| chr1.3 | exon | 18720214 | 18720266 | 18720214 | ID=MS.gene061658.t1.exon3;Parent=MS.gene061658.t1 |
| chr1.3 | CDS | 18720214 | 18720266 | 18720214 | ID=cds.MS.gene061658.t1;Parent=MS.gene061658.t1 |
| chr1.3 | exon | 18721290 | 18721382 | 18721290 | ID=MS.gene061658.t1.exon4;Parent=MS.gene061658.t1 |
| chr1.3 | CDS | 18721290 | 18721382 | 18721290 | ID=cds.MS.gene061658.t1;Parent=MS.gene061658.t1 |
| chr1.3 | exon | 18721499 | 18721570 | 18721499 | ID=MS.gene061658.t1.exon5;Parent=MS.gene061658.t1 |
| chr1.3 | CDS | 18721499 | 18721570 | 18721499 | ID=cds.MS.gene061658.t1;Parent=MS.gene061658.t1 |
| chr1.3 | exon | 18724185 | 18724257 | 18724185 | ID=MS.gene061658.t1.exon6;Parent=MS.gene061658.t1 |
| chr1.3 | CDS | 18724185 | 18724257 | 18724185 | ID=cds.MS.gene061658.t1;Parent=MS.gene061658.t1 |
| chr1.3 | exon | 18724356 | 18724401 | 18724356 | ID=MS.gene061658.t1.exon7;Parent=MS.gene061658.t1 |
| chr1.3 | CDS | 18724356 | 18724401 | 18724356 | ID=cds.MS.gene061658.t1;Parent=MS.gene061658.t1 |
| chr1.3 | exon | 18724916 | 18724979 | 18724916 | ID=MS.gene061658.t1.exon8;Parent=MS.gene061658.t1 |
| chr1.3 | CDS | 18724916 | 18724979 | 18724916 | ID=cds.MS.gene061658.t1;Parent=MS.gene061658.t1 |
| chr1.3 | exon | 18726361 | 18726414 | 18726361 | ID=MS.gene061658.t1.exon9;Parent=MS.gene061658.t1 |
| chr1.3 | CDS | 18726361 | 18726414 | 18726361 | ID=cds.MS.gene061658.t1;Parent=MS.gene061658.t1 |
| chr1.3 | exon | 18726498 | 18726575 | 18726498 | ID=MS.gene061658.t1.exon10;Parent=MS.gene061658.t1 |
| chr1.3 | CDS | 18726498 | 18726575 | 18726498 | ID=cds.MS.gene061658.t1;Parent=MS.gene061658.t1 |
| Gene Sequence |
| Protein sequence |
