Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067695.t1 | XP_003605528.1 | 97 | 101 | 3 | 0 | 1 | 101 | 99 | 199 | 4.80E-47 | 196.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067695.t1 | Q5P316 | 34.4 | 93 | 60 | 1 | 8 | 100 | 26 | 117 | 2.2e-08 | 59.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067695.t1 | G7JN88 | 97.0 | 101 | 3 | 0 | 1 | 101 | 99 | 199 | 3.4e-47 | 196.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene067695 | 0.806868 | 6.67E-50 | -1.69E-46 |
| MS.gene049284 | MS.gene067695 | 0.804057 | 2.59E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene067695 | 0.842159 | 3.06E-58 | -1.69E-46 |
| MS.gene050301 | MS.gene067695 | 0.844168 | 8.93E-59 | -1.69E-46 |
| MS.gene050397 | MS.gene067695 | 0.824977 | 6.03E-54 | -1.69E-46 |
| MS.gene050404 | MS.gene067695 | 0.819368 | 1.20E-52 | -1.69E-46 |
| MS.gene050406 | MS.gene067695 | 0.820269 | 7.50E-53 | -1.69E-46 |
| MS.gene050426 | MS.gene067695 | -0.813705 | 2.24E-51 | -1.69E-46 |
| MS.gene050464 | MS.gene067695 | 0.816923 | 4.30E-52 | -1.69E-46 |
| MS.gene050529 | MS.gene067695 | 0.861208 | 1.21E-63 | -1.69E-46 |
| MS.gene050558 | MS.gene067695 | 0.801947 | 7.08E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene067695 | 0.836554 | 8.74E-57 | -1.69E-46 |
| MS.gene05101 | MS.gene067695 | 0.812204 | 4.77E-51 | -1.69E-46 |
| MS.gene051471 | MS.gene067695 | 0.803772 | 2.97E-49 | -1.69E-46 |
| MS.gene051582 | MS.gene067695 | 0.806531 | 7.86E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene067695 | 0.805123 | 1.55E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene067695 | 0.85267 | 3.96E-61 | -1.69E-46 |
| MS.gene052077 | MS.gene067695 | 0.813968 | 1.96E-51 | -1.69E-46 |
| MS.gene052078 | MS.gene067695 | 0.823272 | 1.51E-53 | -1.69E-46 |
| MS.gene052178 | MS.gene067695 | 0.826337 | 2.87E-54 | -1.69E-46 |
| MS.gene052188 | MS.gene067695 | 0.813171 | 2.93E-51 | -1.69E-46 |
| MS.gene052253 | MS.gene067695 | 0.809659 | 1.70E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene067695 | 0.838368 | 3.00E-57 | -1.69E-46 |
| MS.gene053209 | MS.gene067695 | 0.831557 | 1.56E-55 | -1.69E-46 |
| MS.gene053557 | MS.gene067695 | 0.857099 | 2.06E-62 | -1.69E-46 |
| MS.gene053836 | MS.gene067695 | 0.847994 | 8.13E-60 | -1.69E-46 |
| MS.gene053907 | MS.gene067695 | 0.806629 | 7.50E-50 | -1.69E-46 |
| MS.gene053971 | MS.gene067695 | 0.802713 | 4.92E-49 | -1.69E-46 |
| MS.gene054983 | MS.gene067695 | 0.807043 | 6.13E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene067695 | 0.808032 | 3.78E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene067695 | 0.815691 | 8.12E-52 | -1.69E-46 |
| MS.gene055422 | MS.gene067695 | 0.832352 | 9.93E-56 | -1.69E-46 |
| MS.gene055428 | MS.gene067695 | 0.832359 | 9.89E-56 | -1.69E-46 |
| MS.gene05553 | MS.gene067695 | 0.803009 | 4.28E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene067695 | 0.806432 | 8.25E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene067695 | 0.847669 | 9.99E-60 | -1.69E-46 |
| MS.gene056659 | MS.gene067695 | 0.825505 | 4.52E-54 | -1.69E-46 |
| MS.gene056796 | MS.gene067695 | 0.80984 | 1.55E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene067695 | 0.84787 | 8.80E-60 | -1.69E-46 |
| MS.gene05702 | MS.gene067695 | 0.836698 | 8.03E-57 | -1.69E-46 |
| MS.gene057121 | MS.gene067695 | 0.831708 | 1.43E-55 | -1.69E-46 |
| MS.gene057439 | MS.gene067695 | 0.802288 | 6.02E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene067695 | 0.836792 | 7.60E-57 | -1.69E-46 |
| MS.gene058082 | MS.gene067695 | 0.839536 | 1.49E-57 | -1.69E-46 |
| MS.gene05811 | MS.gene067695 | 0.836317 | 1.00E-56 | -1.69E-46 |
| MS.gene058142 | MS.gene067695 | 0.860642 | 1.79E-63 | -1.69E-46 |
| MS.gene058233 | MS.gene067695 | 0.824203 | 9.17E-54 | -1.69E-46 |
| MS.gene058276 | MS.gene067695 | 0.862131 | 6.30E-64 | -1.69E-46 |
| MS.gene058710 | MS.gene067695 | 0.800322 | 1.52E-48 | -1.69E-46 |
| MS.gene058746 | MS.gene067695 | 0.800055 | 1.72E-48 | -1.69E-46 |
| MS.gene058845 | MS.gene067695 | 0.805981 | 1.03E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene067695 | 0.820782 | 5.72E-53 | -1.69E-46 |
| MS.gene059518 | MS.gene067695 | 0.829466 | 5.07E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene067695 | 0.813129 | 2.99E-51 | -1.69E-46 |
| MS.gene059692 | MS.gene067695 | 0.825633 | 4.21E-54 | -1.69E-46 |
| MS.gene059695 | MS.gene067695 | 0.821078 | 4.89E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene067695 | 0.844256 | 8.46E-59 | -1.69E-46 |
| MS.gene059894 | MS.gene067695 | 0.856197 | 3.79E-62 | -1.69E-46 |
| MS.gene059899 | MS.gene067695 | 0.813733 | 2.20E-51 | -1.69E-46 |
| MS.gene060407 | MS.gene067695 | 0.80827 | 3.36E-50 | -1.69E-46 |
| MS.gene06090 | MS.gene067695 | 0.804865 | 1.76E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene067695 | 0.847112 | 1.42E-59 | -1.69E-46 |
| MS.gene061159 | MS.gene067695 | 0.807522 | 4.85E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067695.t1 | MTR_4g032660 | 97.030 | 101 | 3 | 0 | 1 | 101 | 99 | 199 | 6.04e-70 | 207 |
| MS.gene067695.t1 | MTR_3g012540 | 48.454 | 97 | 50 | 0 | 2 | 98 | 84 | 180 | 3.11e-28 | 101 |
| MS.gene067695.t1 | MTR_5g006310 | 41.237 | 97 | 57 | 0 | 2 | 98 | 240 | 336 | 1.49e-21 | 87.4 |
| MS.gene067695.t1 | MTR_1g114200 | 32.990 | 97 | 65 | 0 | 2 | 98 | 15 | 111 | 1.42e-17 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067695.t1 | AT3G20230 | 73.267 | 101 | 27 | 0 | 1 | 101 | 86 | 186 | 1.49e-54 | 168 |
| MS.gene067695.t1 | AT1G08845 | 46.392 | 97 | 52 | 0 | 2 | 98 | 86 | 182 | 5.23e-28 | 100 |
| MS.gene067695.t1 | AT1G08845 | 46.392 | 97 | 52 | 0 | 2 | 98 | 86 | 182 | 5.23e-28 | 100 |
| MS.gene067695.t1 | AT1G08845 | 46.392 | 97 | 52 | 0 | 2 | 98 | 131 | 227 | 7.13e-28 | 101 |
| MS.gene067695.t1 | AT5G27820 | 34.021 | 97 | 64 | 0 | 2 | 98 | 15 | 111 | 6.67e-19 | 75.5 |
| MS.gene067695.t1 | AT3G22450 | 42.553 | 94 | 54 | 0 | 5 | 98 | 197 | 290 | 5.37e-15 | 68.9 |
| MS.gene067695.t1 | AT3G22450 | 42.553 | 94 | 54 | 0 | 5 | 98 | 197 | 290 | 5.37e-15 | 68.9 |
Find 17 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGAAGCTTGTAGAGTAAT+TGG | 0.243088 | 4.1:-68369245 | MS.gene067695:CDS |
| TCAATCCTTTCATTCTTTCT+CGG | 0.316725 | 4.1:+68369167 | None:intergenic |
| CCAATTACTCTACAAGCTTC+AGG | 0.324712 | 4.1:+68369245 | None:intergenic |
| AAGAGAGTTTCGAAGATCTT+TGG | 0.330634 | 4.1:+68369283 | None:intergenic |
| CTTGATACTGTTAAAGAAAA+CGG | 0.356290 | 4.1:-68369125 | MS.gene067695:CDS |
| TTGATACTGTTAAAGAAAAC+GGG | 0.383944 | 4.1:-68369124 | MS.gene067695:CDS |
| TCGAAGATCTTTGGCGTTTG+TGG | 0.436613 | 4.1:+68369292 | None:intergenic |
| AGAAAGAATGAAAGGATTGA+AGG | 0.478137 | 4.1:-68369164 | MS.gene067695:CDS |
| GAAAGGATTGAAGGTAGACT+TGG | 0.492962 | 4.1:-68369155 | MS.gene067695:CDS |
| ATATCACTTTGCTTGTTCCA+CGG | 0.510627 | 4.1:+68369324 | None:intergenic |
| ACAAGCTTCAGGATCTATCA+AGG | 0.541521 | 4.1:+68369256 | None:intergenic |
| GCTTCAGGATCTATCAAGGA+TGG | 0.567230 | 4.1:+68369260 | None:intergenic |
| GCTTATTGCAGAGCGATCAA+AGG | 0.597674 | 4.1:-68369219 | MS.gene067695:CDS |
| ACGAACCGAGAAAGAATGAA+AGG | 0.603027 | 4.1:-68369172 | MS.gene067695:CDS |
| AAGCTTGTAGAGTAATTGGA+AGG | 0.614586 | 4.1:-68369241 | MS.gene067695:CDS |
| GGAAGCTGATGTATATGCAA+TGG | 0.615199 | 4.1:-68369198 | MS.gene067695:CDS |
| CACGCGAAAGTGACACACCG+TGG | 0.691365 | 4.1:-68369341 | MS.gene067695:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAAATGTCTAATAAAAA+CGG | + | chr4.1:68369124-68369143 | None:intergenic | 15.0% |
| ! | CTTGATACTGTTAAAGAAAA+CGG | - | chr4.1:68369376-68369395 | MS.gene067695:CDS | 25.0% |
| ! | TTGATACTGTTAAAGAAAAC+GGG | - | chr4.1:68369377-68369396 | MS.gene067695:CDS | 25.0% |
| AGAAAGAATGAAAGGATTGA+AGG | - | chr4.1:68369337-68369356 | MS.gene067695:CDS | 30.0% | |
| TCAATCCTTTCATTCTTTCT+CGG | + | chr4.1:68369337-68369356 | None:intergenic | 30.0% | |
| AAGAGAGTTTCGAAGATCTT+TGG | + | chr4.1:68369221-68369240 | None:intergenic | 35.0% | |
| AAGCTTGTAGAGTAATTGGA+AGG | - | chr4.1:68369260-68369279 | MS.gene067695:CDS | 35.0% | |
| ATATCACTTTGCTTGTTCCA+CGG | + | chr4.1:68369180-68369199 | None:intergenic | 35.0% | |
| ACAAGCTTCAGGATCTATCA+AGG | + | chr4.1:68369248-68369267 | None:intergenic | 40.0% | |
| ACGAACCGAGAAAGAATGAA+AGG | - | chr4.1:68369329-68369348 | MS.gene067695:CDS | 40.0% | |
| CCAATTACTCTACAAGCTTC+AGG | + | chr4.1:68369259-68369278 | None:intergenic | 40.0% | |
| CCTGAAGCTTGTAGAGTAAT+TGG | - | chr4.1:68369256-68369275 | MS.gene067695:CDS | 40.0% | |
| GAAAGGATTGAAGGTAGACT+TGG | - | chr4.1:68369346-68369365 | MS.gene067695:CDS | 40.0% | |
| GGAAGCTGATGTATATGCAA+TGG | - | chr4.1:68369303-68369322 | MS.gene067695:CDS | 40.0% | |
| GCTTATTGCAGAGCGATCAA+AGG | - | chr4.1:68369282-68369301 | MS.gene067695:CDS | 45.0% | |
| GCTTCAGGATCTATCAAGGA+TGG | + | chr4.1:68369244-68369263 | None:intergenic | 45.0% | |
| TCGAAGATCTTTGGCGTTTG+TGG | + | chr4.1:68369212-68369231 | None:intergenic | 45.0% | |
| CACGCGAAAGTGACACACCG+TGG | - | chr4.1:68369160-68369179 | MS.gene067695:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 68369109 | 68369414 | 68369109 | ID=MS.gene067695 |
| chr4.1 | mRNA | 68369109 | 68369414 | 68369109 | ID=MS.gene067695.t1;Parent=MS.gene067695 |
| chr4.1 | exon | 68369109 | 68369414 | 68369109 | ID=MS.gene067695.t1.exon1;Parent=MS.gene067695.t1 |
| chr4.1 | CDS | 68369109 | 68369414 | 68369109 | ID=cds.MS.gene067695.t1;Parent=MS.gene067695.t1 |
| Gene Sequence |
| Protein sequence |
