Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072955.t1 | XP_003603465.1 | 97.3 | 405 | 11 | 0 | 1 | 405 | 802 | 1206 | 9.30E-227 | 795.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072955.t1 | Q9FWQ5 | 41.1 | 411 | 218 | 6 | 1 | 397 | 1292 | 1692 | 7.7e-89 | 328.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072955.t1 | G7JBQ0 | 97.3 | 405 | 11 | 0 | 1 | 405 | 802 | 1206 | 6.7e-227 | 795.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene072955.t1 | TR | TAZ |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049293 | MS.gene072955 | -0.811394 | 7.16E-51 | -1.69E-46 |
| MS.gene049378 | MS.gene072955 | 0.807995 | 3.85E-50 | -1.69E-46 |
| MS.gene049669 | MS.gene072955 | 0.83133 | 1.77E-55 | -1.69E-46 |
| MS.gene049825 | MS.gene072955 | 0.822427 | 2.38E-53 | -1.69E-46 |
| MS.gene049873 | MS.gene072955 | 0.821346 | 4.24E-53 | -1.69E-46 |
| MS.gene050026 | MS.gene072955 | 0.808714 | 2.70E-50 | -1.69E-46 |
| MS.gene050201 | MS.gene072955 | 0.819152 | 1.35E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene072955 | 0.813099 | 3.04E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene072955 | 0.806342 | 8.62E-50 | -1.69E-46 |
| MS.gene050303 | MS.gene072955 | 0.832887 | 7.31E-56 | -1.69E-46 |
| MS.gene050330 | MS.gene072955 | -0.807843 | 4.15E-50 | -1.69E-46 |
| MS.gene050355 | MS.gene072955 | 0.814029 | 1.90E-51 | -1.69E-46 |
| MS.gene050623 | MS.gene072955 | -0.802352 | 5.84E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene072955 | 0.815507 | 8.92E-52 | -1.69E-46 |
| MS.gene050721 | MS.gene072955 | 0.816644 | 4.97E-52 | -1.69E-46 |
| MS.gene050750 | MS.gene072955 | 0.807083 | 6.01E-50 | -1.69E-46 |
| MS.gene050799 | MS.gene072955 | 0.801997 | 6.91E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene072955 | 0.825676 | 4.12E-54 | -1.69E-46 |
| MS.gene052108 | MS.gene072955 | 0.800387 | 1.48E-48 | -1.69E-46 |
| MS.gene052176 | MS.gene072955 | 0.832039 | 1.19E-55 | -1.69E-46 |
| MS.gene052309 | MS.gene072955 | -0.818121 | 2.31E-52 | -1.69E-46 |
| MS.gene052333 | MS.gene072955 | 0.809012 | 2.33E-50 | -1.69E-46 |
| MS.gene052358 | MS.gene072955 | -0.850457 | 1.68E-60 | -1.69E-46 |
| MS.gene052519 | MS.gene072955 | 0.811066 | 8.43E-51 | -1.69E-46 |
| MS.gene052586 | MS.gene072955 | 0.824663 | 7.14E-54 | -1.69E-46 |
| MS.gene052685 | MS.gene072955 | 0.817803 | 2.73E-52 | -1.69E-46 |
| MS.gene052926 | MS.gene072955 | 0.805562 | 1.26E-49 | -1.69E-46 |
| MS.gene053054 | MS.gene072955 | -0.826323 | 2.89E-54 | -1.69E-46 |
| MS.gene053221 | MS.gene072955 | 0.827102 | 1.88E-54 | -1.69E-46 |
| MS.gene05365 | MS.gene072955 | 0.809487 | 1.85E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene072955 | 0.80887 | 2.50E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene072955 | 0.827394 | 1.60E-54 | -1.69E-46 |
| MS.gene054291 | MS.gene072955 | -0.837732 | 4.37E-57 | -1.69E-46 |
| MS.gene054405 | MS.gene072955 | 0.818259 | 2.15E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene072955 | 0.816676 | 4.89E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene072955 | 0.801365 | 9.32E-49 | -1.69E-46 |
| MS.gene055778 | MS.gene072955 | 0.809131 | 2.20E-50 | -1.69E-46 |
| MS.gene055808 | MS.gene072955 | 0.813667 | 2.28E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene072955 | 0.822634 | 2.13E-53 | -1.69E-46 |
| MS.gene056087 | MS.gene072955 | 0.801694 | 7.98E-49 | -1.69E-46 |
| MS.gene056091 | MS.gene072955 | 0.832527 | 8.99E-56 | -1.69E-46 |
| MS.gene056373 | MS.gene072955 | 0.813087 | 3.06E-51 | -1.69E-46 |
| MS.gene056506 | MS.gene072955 | 0.823564 | 1.29E-53 | -1.69E-46 |
| MS.gene05659 | MS.gene072955 | 0.805314 | 1.42E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene072955 | 0.848159 | 7.32E-60 | -1.69E-46 |
| MS.gene057060 | MS.gene072955 | 0.813109 | 3.02E-51 | -1.69E-46 |
| MS.gene057414 | MS.gene072955 | 0.805492 | 1.30E-49 | -1.69E-46 |
| MS.gene057555 | MS.gene072955 | 0.803162 | 3.98E-49 | -1.69E-46 |
| MS.gene05795 | MS.gene072955 | 0.804269 | 2.34E-49 | -1.69E-46 |
| MS.gene057967 | MS.gene072955 | 0.804809 | 1.81E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene072955 | 0.833678 | 4.64E-56 | -1.69E-46 |
| MS.gene058498 | MS.gene072955 | 0.823907 | 1.08E-53 | -1.69E-46 |
| MS.gene058500 | MS.gene072955 | 0.802055 | 6.73E-49 | -1.69E-46 |
| MS.gene058636 | MS.gene072955 | 0.815779 | 7.76E-52 | -1.69E-46 |
| MS.gene05954 | MS.gene072955 | 0.857476 | 1.59E-62 | -1.69E-46 |
| MS.gene060521 | MS.gene072955 | 0.816154 | 6.40E-52 | -1.69E-46 |
| MS.gene060572 | MS.gene072955 | 0.863311 | 2.73E-64 | -1.69E-46 |
| MS.gene060586 | MS.gene072955 | 0.805317 | 1.42E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene072955 | -0.842093 | 3.19E-58 | -1.69E-46 |
| MS.gene060879 | MS.gene072955 | 0.83099 | 2.15E-55 | -1.69E-46 |
| MS.gene061040 | MS.gene072955 | -0.876811 | 1.04E-68 | -1.69E-46 |
| MS.gene061266 | MS.gene072955 | 0.814102 | 1.83E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072955.t1 | MTR_3g107960 | 97.037 | 405 | 12 | 0 | 1 | 405 | 802 | 1206 | 0.0 | 831 |
| MS.gene072955.t1 | MTR_3g101100 | 80.668 | 419 | 67 | 3 | 1 | 405 | 977 | 1395 | 0.0 | 702 |
| MS.gene072955.t1 | MTR_6g017010 | 66.000 | 400 | 84 | 4 | 1 | 398 | 416 | 765 | 0.0 | 528 |
| MS.gene072955.t1 | MTR_5g085310 | 67.609 | 389 | 77 | 3 | 1 | 389 | 686 | 1025 | 1.22e-180 | 530 |
| MS.gene072955.t1 | MTR_5g017020 | 59.394 | 330 | 82 | 4 | 1 | 324 | 242 | 525 | 2.60e-121 | 362 |
| MS.gene072955.t1 | MTR_5g017020 | 51.100 | 409 | 106 | 6 | 1 | 405 | 242 | 560 | 5.24e-120 | 360 |
| MS.gene072955.t1 | MTR_4g005050 | 42.579 | 411 | 211 | 7 | 1 | 397 | 1296 | 1695 | 1.51e-107 | 347 |
| MS.gene072955.t1 | MTR_6g017000 | 66.667 | 228 | 76 | 0 | 1 | 228 | 720 | 947 | 5.79e-100 | 318 |
| MS.gene072955.t1 | MTR_0299s0030 | 54.959 | 242 | 51 | 5 | 28 | 220 | 67 | 299 | 2.18e-74 | 235 |
| MS.gene072955.t1 | MTR_6g017140 | 58.081 | 198 | 64 | 2 | 1 | 196 | 273 | 453 | 2.01e-71 | 231 |
| MS.gene072955.t1 | MTR_5g085317 | 67.677 | 99 | 27 | 2 | 1 | 99 | 1 | 94 | 7.87e-37 | 130 |
| MS.gene072955.t1 | MTR_6g017130 | 59.483 | 116 | 29 | 4 | 142 | 252 | 249 | 351 | 4.84e-33 | 128 |
| MS.gene072955.t1 | MTR_6g017130 | 62.791 | 86 | 27 | 2 | 303 | 387 | 309 | 390 | 2.21e-24 | 103 |
| MS.gene072955.t1 | MTR_1g047940 | 55.238 | 105 | 23 | 1 | 291 | 395 | 118 | 198 | 3.20e-27 | 107 |
| MS.gene072955.t1 | MTR_1g047940 | 71.154 | 52 | 15 | 0 | 15 | 66 | 17 | 68 | 9.27e-18 | 81.6 |
| MS.gene072955.t1 | MTR_6g017135 | 76.316 | 38 | 9 | 0 | 10 | 47 | 103 | 140 | 4.05e-13 | 66.6 |
| MS.gene072955.t1 | MTR_5g015680 | 40.741 | 81 | 46 | 2 | 318 | 397 | 184 | 263 | 8.89e-13 | 68.6 |
| MS.gene072955.t1 | MTR_5g015680 | 40.741 | 81 | 46 | 2 | 318 | 397 | 279 | 358 | 1.37e-12 | 68.9 |
| MS.gene072955.t1 | MTR_5g015680 | 40.741 | 81 | 46 | 2 | 318 | 397 | 318 | 397 | 1.69e-12 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072955.t1 | AT1G16710 | 42.822 | 411 | 211 | 6 | 1 | 397 | 1263 | 1663 | 8.13e-110 | 354 |
| MS.gene072955.t1 | AT1G16710 | 42.822 | 411 | 211 | 6 | 1 | 397 | 1292 | 1692 | 8.66e-110 | 354 |
| MS.gene072955.t1 | AT1G16710 | 42.822 | 411 | 211 | 6 | 1 | 397 | 1227 | 1627 | 8.98e-110 | 353 |
| MS.gene072955.t1 | AT1G16710 | 42.822 | 411 | 211 | 6 | 1 | 397 | 1227 | 1627 | 8.98e-110 | 353 |
| MS.gene072955.t1 | AT1G16710 | 42.822 | 411 | 211 | 6 | 1 | 397 | 1227 | 1627 | 8.98e-110 | 353 |
| MS.gene072955.t1 | AT1G16710 | 42.822 | 411 | 211 | 6 | 1 | 397 | 1266 | 1666 | 9.45e-110 | 353 |
| MS.gene072955.t1 | AT1G16710 | 42.822 | 411 | 211 | 6 | 1 | 397 | 1266 | 1666 | 9.45e-110 | 353 |
| MS.gene072955.t1 | AT1G16710 | 42.822 | 411 | 211 | 6 | 1 | 397 | 1266 | 1666 | 9.45e-110 | 353 |
| MS.gene072955.t1 | AT1G16710 | 42.822 | 411 | 211 | 6 | 1 | 397 | 1266 | 1666 | 9.45e-110 | 353 |
| MS.gene072955.t1 | AT1G79000 | 42.336 | 411 | 213 | 6 | 1 | 397 | 1259 | 1659 | 1.11e-108 | 350 |
| MS.gene072955.t1 | AT1G79000 | 42.336 | 411 | 213 | 6 | 1 | 397 | 1283 | 1683 | 1.27e-108 | 350 |
| MS.gene072955.t1 | AT1G79000 | 42.336 | 411 | 213 | 6 | 1 | 397 | 1283 | 1683 | 1.56e-108 | 350 |
| MS.gene072955.t1 | AT3G12980 | 41.176 | 408 | 214 | 8 | 1 | 397 | 1182 | 1574 | 2.45e-99 | 324 |
| MS.gene072955.t1 | AT3G12980 | 41.176 | 408 | 214 | 8 | 1 | 397 | 1263 | 1655 | 2.82e-99 | 324 |
| MS.gene072955.t1 | AT1G55970 | 37.897 | 409 | 233 | 7 | 1 | 397 | 1058 | 1457 | 3.39e-83 | 278 |
| MS.gene072955.t1 | AT1G55970 | 37.897 | 409 | 233 | 7 | 1 | 397 | 1044 | 1443 | 4.17e-83 | 277 |
| MS.gene072955.t1 | AT1G67220 | 34.165 | 401 | 220 | 13 | 1 | 378 | 982 | 1361 | 4.18e-67 | 231 |
| MS.gene072955.t1 | AT1G16710 | 43.137 | 153 | 84 | 1 | 1 | 150 | 1266 | 1418 | 3.46e-39 | 150 |
| MS.gene072955.t1 | AT5G67480 | 42.308 | 78 | 43 | 2 | 316 | 391 | 282 | 359 | 2.14e-12 | 68.6 |
| MS.gene072955.t1 | AT5G67480 | 42.308 | 78 | 43 | 2 | 316 | 391 | 271 | 348 | 2.15e-12 | 68.6 |
| MS.gene072955.t1 | AT5G67480 | 42.308 | 78 | 43 | 2 | 316 | 391 | 271 | 348 | 2.15e-12 | 68.6 |
Find 75 sgRNAs with CRISPR-Local
Find 123 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTTGTTCCTACTGAAAA+AGG | 0.108559 | 3.1:+85778499 | MS.gene072955:CDS |
| TCAACACCACTATGTCAATT+TGG | 0.177074 | 3.1:+85779698 | MS.gene072955:CDS |
| CACTGAATGCAAGAAATTTC+AGG | 0.225944 | 3.1:+85779033 | MS.gene072955:CDS |
| CCCTGGTAGGCAATATCTTT+TGG | 0.238306 | 3.1:-85778931 | None:intergenic |
| CCAGAGTAGGGTTGTTTAAA+TGG | 0.239132 | 3.1:-85779505 | None:intergenic |
| AGAGTGGATTATTTGAAAAT+AGG | 0.257666 | 3.1:+85779396 | MS.gene072955:CDS |
| TTCCGAGTCTTTGCAATTCC+TGG | 0.259668 | 3.1:-85780017 | None:intergenic |
| TTTGAGTCAACTTGTGTTCA+TGG | 0.292920 | 3.1:-85779649 | None:intergenic |
| ATGGTCTTTAAAGCTCTATT+TGG | 0.306293 | 3.1:-85778655 | None:intergenic |
| TCTCGTTTGCCATACTTTGA+TGG | 0.326312 | 3.1:+85778541 | MS.gene072955:CDS |
| TTGCATCACCAGAATGTCTT+TGG | 0.327789 | 3.1:-85778707 | None:intergenic |
| CATTTGCTCTGCTTGCTATA+AGG | 0.336680 | 3.1:+85779610 | MS.gene072955:CDS |
| TCTTTGGCAGTGTCTTTAGA+AGG | 0.367904 | 3.1:-85778691 | None:intergenic |
| CAACACCACTATGTCAATTT+GGG | 0.370714 | 3.1:+85779699 | MS.gene072955:CDS |
| CAAACTGAAATTGATACTTC+TGG | 0.380328 | 3.1:-85779439 | None:intergenic |
| AGACACTGCCAAAGACATTC+TGG | 0.381560 | 3.1:+85778699 | MS.gene072955:CDS |
| ACTGAAGAAAATATTATTGT+TGG | 0.383324 | 3.1:+85778454 | MS.gene072955:CDS |
| TGCCATGAAGTGATAGCATC+TGG | 0.393848 | 3.1:+85778995 | MS.gene072955:CDS |
| GGCAGTGTCTTTAGAAGGAT+TGG | 0.396884 | 3.1:-85778686 | None:intergenic |
| GGTGCAAAATTCGAGTAAAT+CGG | 0.403532 | 3.1:+85779948 | MS.gene072955:CDS |
| TCCCAGATGCTATCACTTCA+TGG | 0.404024 | 3.1:-85778997 | None:intergenic |
| CAGTAGTCTCCATCAAAGTA+TGG | 0.411615 | 3.1:-85778550 | None:intergenic |
| AATGTAGAATACCACGTTGC+AGG | 0.414844 | 3.1:+85780038 | MS.gene072955:CDS |
| CTACTGGTCTGGTTATGTTA+TGG | 0.415989 | 3.1:+85778567 | MS.gene072955:CDS |
| AAGCAACGCATTCCGAATCT+TGG | 0.427714 | 3.1:+85780268 | MS.gene072955:CDS |
| GTGCAAAATTCGAGTAAATC+GGG | 0.429183 | 3.1:+85779949 | MS.gene072955:CDS |
| TGCAGCTCCACGCCAAGATT+CGG | 0.450882 | 3.1:-85780280 | None:intergenic |
| ACAATATATTTCAAAAGTGC+TGG | 0.451731 | 3.1:+85779561 | MS.gene072955:CDS |
| AAATGTGTGCATCAGAACTA+TGG | 0.452913 | 3.1:-85779204 | None:intergenic |
| GTCTTTGCAATTCCTGGAGT+GGG | 0.453840 | 3.1:-85780011 | None:intergenic |
| GCAGAGCAAATGGTACATTC+TGG | 0.456546 | 3.1:-85779599 | None:intergenic |
| TTCTTGATTTGAGAACAGTT+TGG | 0.463384 | 3.1:-85779905 | None:intergenic |
| TAGGGTTGTTTAAATGGTAG+AGG | 0.474232 | 3.1:-85779499 | None:intergenic |
| AACCCTACTCTGGTGACTGT+TGG | 0.474451 | 3.1:+85779515 | MS.gene072955:CDS |
| GATGCACACATTTCAAAGAA+TGG | 0.475962 | 3.1:+85779214 | MS.gene072955:CDS |
| ATCATTATGCTTAGTGTTGG+AGG | 0.477828 | 3.1:-85779363 | None:intergenic |
| GATATCATTATGCTTAGTGT+TGG | 0.494216 | 3.1:-85779366 | None:intergenic |
| GCCAAAAGATATTGCCTACC+AGG | 0.497791 | 3.1:+85778930 | MS.gene072955:CDS |
| CGAGGTGCATACACTATGTC+AGG | 0.500355 | 3.1:+85779237 | MS.gene072955:CDS |
| CTTATAGCAAGCAGAGCAAA+TGG | 0.504219 | 3.1:-85779609 | None:intergenic |
| TCGTGAGTCAAATGAGAAAA+CGG | 0.506997 | 3.1:+85779724 | MS.gene072955:CDS |
| TTATACTAACTGCCCACTCC+AGG | 0.508191 | 3.1:+85779999 | MS.gene072955:CDS |
| TTTGATGGAGACTACTGGTC+TGG | 0.509335 | 3.1:+85778556 | MS.gene072955:CDS |
| CACGCAGATGCTGAAACTGC+TGG | 0.511708 | 3.1:+85779826 | MS.gene072955:intron |
| GATCATCATTGATGAATACT+TGG | 0.516147 | 3.1:-85779477 | None:intergenic |
| CTCCAGGAATTGCAAAGACT+CGG | 0.528433 | 3.1:+85780015 | MS.gene072955:CDS |
| AGAAATTTCAGGAGTGTGAA+AGG | 0.528641 | 3.1:+85779044 | MS.gene072955:CDS |
| TTCCAACAGTCACCAGAGTA+GGG | 0.530507 | 3.1:-85779517 | None:intergenic |
| TTTCCAACAGTCACCAGAGT+AGG | 0.537493 | 3.1:-85779518 | None:intergenic |
| CCATTTAAACAACCCTACTC+TGG | 0.546250 | 3.1:+85779505 | MS.gene072955:CDS |
| AGTCTTTGCAATTCCTGGAG+TGG | 0.547250 | 3.1:-85780012 | None:intergenic |
| TCTGCTTGCTATAAGGATAG+AGG | 0.547525 | 3.1:+85779617 | MS.gene072955:CDS |
| TTTCAAATAATCCACTCTCA+AGG | 0.548304 | 3.1:-85779391 | None:intergenic |
| AGAAGGATTGGCATGTCCCA+TGG | 0.548735 | 3.1:-85778674 | None:intergenic |
| CATACTTTGATGGAGACTAC+TGG | 0.556982 | 3.1:+85778551 | MS.gene072955:CDS |
| CGATTCCCAAATTGACATAG+TGG | 0.580947 | 3.1:-85779704 | None:intergenic |
| TCATTATGCTTAGTGTTGGA+GGG | 0.581798 | 3.1:-85779362 | None:intergenic |
| TGATAGCATCTGGGAAGCGA+TGG | 0.590031 | 3.1:+85779005 | MS.gene072955:CDS |
| GAACAGTTTGGATAAGAGCA+TGG | 0.592137 | 3.1:-85779893 | None:intergenic |
| CAAACGAGAAGTTGTCACCT+TGG | 0.592914 | 3.1:-85778527 | None:intergenic |
| AAATAGAGCTTTAAAGACCA+TGG | 0.593540 | 3.1:+85778657 | MS.gene072955:CDS |
| GTTATGTTATGGAAGCAGCC+AGG | 0.598981 | 3.1:+85778578 | MS.gene072955:CDS |
| AATGATATCATCCTTGAGAG+TGG | 0.608883 | 3.1:+85779380 | MS.gene072955:CDS |
| GCCATGAAGTGATAGCATCT+GGG | 0.616414 | 3.1:+85778996 | MS.gene072955:CDS |
| AATAGAGCTTTAAAGACCAT+GGG | 0.619881 | 3.1:+85778658 | MS.gene072955:CDS |
| CCAAAAGATATTGCCTACCA+GGG | 0.627033 | 3.1:+85778931 | MS.gene072955:CDS |
| GCATGGTTCAGCATTAGTTG+CGG | 0.636133 | 3.1:-85779876 | None:intergenic |
| AAAGAAATGATCATATACGT+TGG | 0.641068 | 3.1:-85778482 | None:intergenic |
| TCATTGATGAATACTTGGCA+CGG | 0.650719 | 3.1:-85779472 | None:intergenic |
| TGCAAAATTCGAGTAAATCG+GGG | 0.651340 | 3.1:+85779950 | MS.gene072955:CDS |
| AGCTATTCTCTCATGCTCGT+AGG | 0.662256 | 3.1:+85779927 | MS.gene072955:CDS |
| ACGCATTCCGAATCTTGGCG+TGG | 0.671085 | 3.1:+85780273 | MS.gene072955:CDS |
| ACACATTTCAAAGAATGGCG+AGG | 0.706634 | 3.1:+85779219 | MS.gene072955:CDS |
| TTGATGAATACTTGGCACGG+CGG | 0.746659 | 3.1:-85779469 | None:intergenic |
| ACAGTCACACACCTGCAACG+TGG | 0.756031 | 3.1:-85780049 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAAATAAATATAAAGAAAT+AGG | - | chr3.1:85780213-85780232 | None:intergenic | 10.0% |
| !!! | ATATTTATTTTGATTTTGCA+GGG | + | chr3.1:85780221-85780240 | MS.gene072955:intron | 15.0% |
| !!! | TATATTTATTTTGATTTTGC+AGG | + | chr3.1:85780220-85780239 | MS.gene072955:intron | 15.0% |
| !! | AAGTATGTATTCATTTCAAA+GGG | - | chr3.1:85779113-85779132 | None:intergenic | 20.0% |
| !! | ACTGAAGAAAATATTATTGT+TGG | + | chr3.1:85778454-85778473 | MS.gene072955:CDS | 20.0% |
| !! | ATAAAAGAATATATCCCATT+GGG | - | chr3.1:85778786-85778805 | None:intergenic | 20.0% |
| !! | ATCATGTAAATAACTTAAAC+TGG | - | chr3.1:85778848-85778867 | None:intergenic | 20.0% |
| !! | TATAAAAGAATATATCCCAT+TGG | - | chr3.1:85778787-85778806 | None:intergenic | 20.0% |
| ! | AAAAAAATGACTGTTGTGAA+AGG | - | chr3.1:85780168-85780187 | None:intergenic | 25.0% |
| ! | AAAGAAATGATCATATACGT+TGG | - | chr3.1:85778485-85778504 | None:intergenic | 25.0% |
| ! | AAATGAATACATACTTGAAC+TGG | + | chr3.1:85779117-85779136 | MS.gene072955:intron | 25.0% |
| ! | AGCCAAAAAATAAATCAGAA+AGG | - | chr3.1:85780121-85780140 | None:intergenic | 25.0% |
| ! | AGTAATTTCATGCAAAACAT+TGG | + | chr3.1:85779303-85779322 | MS.gene072955:intron | 25.0% |
| ! | CAAATTCTCTCAAATTCTAT+AGG | - | chr3.1:85780090-85780109 | None:intergenic | 25.0% |
| ! | CAAGTATGTATTCATTTCAA+AGG | - | chr3.1:85779114-85779133 | None:intergenic | 25.0% |
| ! | CTATAGAATTTGAGAGAATT+TGG | + | chr3.1:85780088-85780107 | MS.gene072955:intron | 25.0% |
| ! | GAAAATCATATGAAAACTTC+AGG | - | chr3.1:85779275-85779294 | None:intergenic | 25.0% |
| !! | ACAATATATTTCAAAAGTGC+TGG | + | chr3.1:85779561-85779580 | MS.gene072955:CDS | 25.0% |
| !!! | AGAGTGGATTATTTGAAAAT+AGG | + | chr3.1:85779396-85779415 | MS.gene072955:CDS | 25.0% |
| !!! | CTCCTTTCTGATTTATTTTT+TGG | + | chr3.1:85780116-85780135 | MS.gene072955:intron | 25.0% |
| CAAACTGAAATTGATACTTC+TGG | - | chr3.1:85779442-85779461 | None:intergenic | 30.0% | |
| GATATCATTATGCTTAGTGT+TGG | - | chr3.1:85779369-85779388 | None:intergenic | 30.0% | |
| GATCATCATTGATGAATACT+TGG | - | chr3.1:85779480-85779499 | None:intergenic | 30.0% | |
| TATGTATTCATTTCAAAGGG+AGG | - | chr3.1:85779110-85779129 | None:intergenic | 30.0% | |
| TCTTTGTTCCTACTGAAAAA+GGG | + | chr3.1:85778500-85778519 | MS.gene072955:CDS | 30.0% | |
| TGCATTGAGTGCAATAAAAA+AGG | + | chr3.1:85779143-85779162 | MS.gene072955:intron | 30.0% | |
| TTCTTTGTTCCTACTGAAAA+AGG | + | chr3.1:85778499-85778518 | MS.gene072955:CDS | 30.0% | |
| TTTCAAATAATCCACTCTCA+AGG | - | chr3.1:85779394-85779413 | None:intergenic | 30.0% | |
| ! | CATATGAAAACTTCAGGTTT+AGG | - | chr3.1:85779269-85779288 | None:intergenic | 30.0% |
| ! | GTTGATGAAAGTAAAAGACA+AGG | - | chr3.1:85778745-85778764 | None:intergenic | 30.0% |
| ! | TTCTTGATTTGAGAACAGTT+TGG | - | chr3.1:85779908-85779927 | None:intergenic | 30.0% |
| !! | AAATAGAGCTTTAAAGACCA+TGG | + | chr3.1:85778657-85778676 | MS.gene072955:CDS | 30.0% |
| !! | AATAGAGCTTTAAAGACCAT+GGG | + | chr3.1:85778658-85778677 | MS.gene072955:CDS | 30.0% |
| !! | ATGGTCTTTAAAGCTCTATT+TGG | - | chr3.1:85778658-85778677 | None:intergenic | 30.0% |
| !!! | TCACAACAGTCATTTTTTTG+TGG | + | chr3.1:85780169-85780188 | MS.gene072955:intron | 30.0% |
| AAATGTGTGCATCAGAACTA+TGG | - | chr3.1:85779207-85779226 | None:intergenic | 35.0% | |
| AATGATATCATCCTTGAGAG+TGG | + | chr3.1:85779380-85779399 | MS.gene072955:CDS | 35.0% | |
| AATTGGGGGGAAAAAAGAAA+AGG | - | chr3.1:85778898-85778917 | None:intergenic | 35.0% | |
| ACGGTATGAACTTAATTCTC+AGG | + | chr3.1:85779743-85779762 | MS.gene072955:intron | 35.0% | |
| ACTTCTGGCAAAAACTCAAA+AGG | - | chr3.1:85779427-85779446 | None:intergenic | 35.0% | |
| CAACACCACTATGTCAATTT+GGG | + | chr3.1:85779699-85779718 | MS.gene072955:CDS | 35.0% | |
| CACTGAATGCAAGAAATTTC+AGG | + | chr3.1:85779033-85779052 | MS.gene072955:CDS | 35.0% | |
| GATGCACACATTTCAAAGAA+TGG | + | chr3.1:85779214-85779233 | MS.gene072955:CDS | 35.0% | |
| GGGAAAAAAGAAAAGGAAGA+AGG | - | chr3.1:85778891-85778910 | None:intergenic | 35.0% | |
| GGTGCAAAATTCGAGTAAAT+CGG | + | chr3.1:85779948-85779967 | MS.gene072955:CDS | 35.0% | |
| GTGCAAAATTCGAGTAAATC+GGG | + | chr3.1:85779949-85779968 | MS.gene072955:CDS | 35.0% | |
| TAGGGTTGTTTAAATGGTAG+AGG | - | chr3.1:85779502-85779521 | None:intergenic | 35.0% | |
| TCAACACCACTATGTCAATT+TGG | + | chr3.1:85779698-85779717 | MS.gene072955:CDS | 35.0% | |
| TCATTGATGAATACTTGGCA+CGG | - | chr3.1:85779475-85779494 | None:intergenic | 35.0% | |
| TCGTGAGTCAAATGAGAAAA+CGG | + | chr3.1:85779724-85779743 | MS.gene072955:CDS | 35.0% | |
| TGCAAAATTCGAGTAAATCG+GGG | + | chr3.1:85779950-85779969 | MS.gene072955:CDS | 35.0% | |
| TGGCCAACCTAATGAAAATT+GGG | - | chr3.1:85778914-85778933 | None:intergenic | 35.0% | |
| TTGGCCAACCTAATGAAAAT+TGG | - | chr3.1:85778915-85778934 | None:intergenic | 35.0% | |
| TTTGAGTCAACTTGTGTTCA+TGG | - | chr3.1:85779652-85779671 | None:intergenic | 35.0% | |
| ! | ACCATATCTTTTTACAGTGC+TGG | - | chr3.1:85779979-85779998 | None:intergenic | 35.0% |
| ! | AGAAATTTCAGGAGTGTGAA+AGG | + | chr3.1:85779044-85779063 | MS.gene072955:CDS | 35.0% |
| ! | ATCATTATGCTTAGTGTTGG+AGG | - | chr3.1:85779366-85779385 | None:intergenic | 35.0% |
| ! | TCATTATGCTTAGTGTTGGA+GGG | - | chr3.1:85779365-85779384 | None:intergenic | 35.0% |
| ! | TTTTCCCCCCAATTTTCATT+AGG | + | chr3.1:85778904-85778923 | MS.gene072955:intron | 35.0% |
| AATGTAGAATACCACGTTGC+AGG | + | chr3.1:85780038-85780057 | MS.gene072955:CDS | 40.0% | |
| ACACATTTCAAAGAATGGCG+AGG | + | chr3.1:85779219-85779238 | MS.gene072955:CDS | 40.0% | |
| AGGACCATTGAAAAAGAGAG+CGG | + | chr3.1:85778598-85778617 | MS.gene072955:CDS | 40.0% | |
| ATCCCATTGGGTGACTATAA+AGG | - | chr3.1:85778774-85778793 | None:intergenic | 40.0% | |
| CAGTAGTCTCCATCAAAGTA+TGG | - | chr3.1:85778553-85778572 | None:intergenic | 40.0% | |
| CATACTTTGATGGAGACTAC+TGG | + | chr3.1:85778551-85778570 | MS.gene072955:CDS | 40.0% | |
| CCAAAAGATATTGCCTACCA+GGG | + | chr3.1:85778931-85778950 | MS.gene072955:CDS | 40.0% | |
| CCAACCTAATGAAAATTGGG+GGG | - | chr3.1:85778911-85778930 | None:intergenic | 40.0% | |
| CCAGAGTAGGGTTGTTTAAA+TGG | - | chr3.1:85779508-85779527 | None:intergenic | 40.0% | |
| CCATTTAAACAACCCTACTC+TGG | + | chr3.1:85779505-85779524 | MS.gene072955:CDS | 40.0% | |
| CGATTCCCAAATTGACATAG+TGG | - | chr3.1:85779707-85779726 | None:intergenic | 40.0% | |
| CTTATAGCAAGCAGAGCAAA+TGG | - | chr3.1:85779612-85779631 | None:intergenic | 40.0% | |
| GAACAGTTTGGATAAGAGCA+TGG | - | chr3.1:85779896-85779915 | None:intergenic | 40.0% | |
| GCCAACCTAATGAAAATTGG+GGG | - | chr3.1:85778912-85778931 | None:intergenic | 40.0% | |
| GCCAGCACTGTAAAAAGATA+TGG | + | chr3.1:85779975-85779994 | MS.gene072955:CDS | 40.0% | |
| GGCCAACCTAATGAAAATTG+GGG | - | chr3.1:85778913-85778932 | None:intergenic | 40.0% | |
| TACTGAAAAAGGGAACTCCA+AGG | + | chr3.1:85778510-85778529 | MS.gene072955:CDS | 40.0% | |
| TCCCATTGGGTGACTATAAA+GGG | - | chr3.1:85778773-85778792 | None:intergenic | 40.0% | |
| TCCCTTTATAGTCACCCAAT+GGG | + | chr3.1:85778769-85778788 | MS.gene072955:intron | 40.0% | |
| TCTCGTTTGCCATACTTTGA+TGG | + | chr3.1:85778541-85778560 | MS.gene072955:CDS | 40.0% | |
| TCTTTGGCAGTGTCTTTAGA+AGG | - | chr3.1:85778694-85778713 | None:intergenic | 40.0% | |
| TTCCCTTTATAGTCACCCAA+TGG | + | chr3.1:85778768-85778787 | MS.gene072955:intron | 40.0% | |
| TTGCATCACCAGAATGTCTT+TGG | - | chr3.1:85778710-85778729 | None:intergenic | 40.0% | |
| ! | AGCTATGATGAAGTTTTCCC+TGG | - | chr3.1:85778951-85778970 | None:intergenic | 40.0% |
| ! | ATGATGAAGTTTTCCCTGGT+AGG | - | chr3.1:85778947-85778966 | None:intergenic | 40.0% |
| ! | CTACTGGTCTGGTTATGTTA+TGG | + | chr3.1:85778567-85778586 | MS.gene072955:CDS | 40.0% |
| !! | CATTTGCTCTGCTTGCTATA+AGG | + | chr3.1:85779610-85779629 | MS.gene072955:CDS | 40.0% |
| !! | TCTGCTTGCTATAAGGATAG+AGG | + | chr3.1:85779617-85779636 | MS.gene072955:CDS | 40.0% |
| !!! | TTGGAGTTCCCTTTTTCAGT+AGG | - | chr3.1:85778511-85778530 | None:intergenic | 40.0% |
| !!! | TTGGATTGTGTGCTGTTTTC+AGG | + | chr3.1:85779322-85779341 | MS.gene072955:intron | 40.0% |
| AAGCAACGCATTCCGAATCT+TGG | + | chr3.1:85780268-85780287 | MS.gene072955:CDS | 45.0% | |
| ACCATTGAAAAAGAGAGCGG+AGG | + | chr3.1:85778601-85778620 | MS.gene072955:CDS | 45.0% | |
| AGACACTGCCAAAGACATTC+TGG | + | chr3.1:85778699-85778718 | MS.gene072955:CDS | 45.0% | |
| AGCTATTCTCTCATGCTCGT+AGG | + | chr3.1:85779927-85779946 | MS.gene072955:CDS | 45.0% | |
| AGTCTTTGCAATTCCTGGAG+TGG | - | chr3.1:85780015-85780034 | None:intergenic | 45.0% | |
| CAAACGAGAAGTTGTCACCT+TGG | - | chr3.1:85778530-85778549 | None:intergenic | 45.0% | |
| CTCCAGGAATTGCAAAGACT+CGG | + | chr3.1:85780015-85780034 | MS.gene072955:CDS | 45.0% | |
| GCAGAGCAAATGGTACATTC+TGG | - | chr3.1:85779602-85779621 | None:intergenic | 45.0% | |
| GCATGGTTCAGCATTAGTTG+CGG | - | chr3.1:85779879-85779898 | None:intergenic | 45.0% | |
| GCCAAAAGATATTGCCTACC+AGG | + | chr3.1:85778930-85778949 | MS.gene072955:CDS | 45.0% | |
| GCCATGAAGTGATAGCATCT+GGG | + | chr3.1:85778996-85779015 | MS.gene072955:CDS | 45.0% | |
| GTCTTTGCAATTCCTGGAGT+GGG | - | chr3.1:85780014-85780033 | None:intergenic | 45.0% | |
| GTTATGTTATGGAAGCAGCC+AGG | + | chr3.1:85778578-85778597 | MS.gene072955:CDS | 45.0% | |
| TCCCAGATGCTATCACTTCA+TGG | - | chr3.1:85779000-85779019 | None:intergenic | 45.0% | |
| TGCCATGAAGTGATAGCATC+TGG | + | chr3.1:85778995-85779014 | MS.gene072955:CDS | 45.0% | |
| TTATACTAACTGCCCACTCC+AGG | + | chr3.1:85779999-85780018 | MS.gene072955:CDS | 45.0% | |
| TTCCAACAGTCACCAGAGTA+GGG | - | chr3.1:85779520-85779539 | None:intergenic | 45.0% | |
| TTCCGAGTCTTTGCAATTCC+TGG | - | chr3.1:85780020-85780039 | None:intergenic | 45.0% | |
| TTGATGAATACTTGGCACGG+CGG | - | chr3.1:85779472-85779491 | None:intergenic | 45.0% | |
| TTTCCAACAGTCACCAGAGT+AGG | - | chr3.1:85779521-85779540 | None:intergenic | 45.0% | |
| TTTGATGGAGACTACTGGTC+TGG | + | chr3.1:85778556-85778575 | MS.gene072955:CDS | 45.0% | |
| ! | CCCCCCAATTTTCATTAGGT+TGG | + | chr3.1:85778908-85778927 | MS.gene072955:intron | 45.0% |
| ! | CCCTGGTAGGCAATATCTTT+TGG | - | chr3.1:85778934-85778953 | None:intergenic | 45.0% |
| ! | GGCAGTGTCTTTAGAAGGAT+TGG | - | chr3.1:85778689-85778708 | None:intergenic | 45.0% |
| !!! | GCTCTCTTTTTCAATGGTCC+TGG | - | chr3.1:85778599-85778618 | None:intergenic | 45.0% |
| !!! | TCCTCCGCTCTCTTTTTCAA+TGG | - | chr3.1:85778605-85778624 | None:intergenic | 45.0% |
| AACCCTACTCTGGTGACTGT+TGG | + | chr3.1:85779515-85779534 | MS.gene072955:CDS | 50.0% | |
| CGAGGTGCATACACTATGTC+AGG | + | chr3.1:85779237-85779256 | MS.gene072955:CDS | 50.0% | |
| TGATAGCATCTGGGAAGCGA+TGG | + | chr3.1:85779005-85779024 | MS.gene072955:CDS | 50.0% | |
| ! | AGAAGGATTGGCATGTCCCA+TGG | - | chr3.1:85778677-85778696 | None:intergenic | 50.0% |
| ACAGTCACACACCTGCAACG+TGG | - | chr3.1:85780052-85780071 | None:intergenic | 55.0% | |
| ACGCATTCCGAATCTTGGCG+TGG | + | chr3.1:85780273-85780292 | MS.gene072955:CDS | 55.0% | |
| CACGCAGATGCTGAAACTGC+TGG | + | chr3.1:85779826-85779845 | MS.gene072955:intron | 55.0% | |
| TGCAGCTCCACGCCAAGATT+CGG | - | chr3.1:85780283-85780302 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 85778439 | 85780338 | 85778439 | ID=MS.gene072955 |
| chr3.1 | mRNA | 85778439 | 85780338 | 85778439 | ID=MS.gene072955.t1;Parent=MS.gene072955 |
| chr3.1 | exon | 85778439 | 85778732 | 85778439 | ID=MS.gene072955.t1.exon1;Parent=MS.gene072955.t1 |
| chr3.1 | CDS | 85778439 | 85778732 | 85778439 | ID=cds.MS.gene072955.t1;Parent=MS.gene072955.t1 |
| chr3.1 | exon | 85778926 | 85779065 | 85778926 | ID=MS.gene072955.t1.exon2;Parent=MS.gene072955.t1 |
| chr3.1 | CDS | 85778926 | 85779065 | 85778926 | ID=cds.MS.gene072955.t1;Parent=MS.gene072955.t1 |
| chr3.1 | exon | 85779201 | 85779258 | 85779201 | ID=MS.gene072955.t1.exon3;Parent=MS.gene072955.t1 |
| chr3.1 | CDS | 85779201 | 85779258 | 85779201 | ID=cds.MS.gene072955.t1;Parent=MS.gene072955.t1 |
| chr3.1 | exon | 85779344 | 85779745 | 85779344 | ID=MS.gene072955.t1.exon4;Parent=MS.gene072955.t1 |
| chr3.1 | CDS | 85779344 | 85779745 | 85779344 | ID=cds.MS.gene072955.t1;Parent=MS.gene072955.t1 |
| chr3.1 | exon | 85779833 | 85780059 | 85779833 | ID=MS.gene072955.t1.exon5;Parent=MS.gene072955.t1 |
| chr3.1 | CDS | 85779833 | 85780059 | 85779833 | ID=cds.MS.gene072955.t1;Parent=MS.gene072955.t1 |
| chr3.1 | exon | 85780242 | 85780338 | 85780242 | ID=MS.gene072955.t1.exon6;Parent=MS.gene072955.t1 |
| chr3.1 | CDS | 85780242 | 85780338 | 85780242 | ID=cds.MS.gene072955.t1;Parent=MS.gene072955.t1 |
| Gene Sequence |
| Protein sequence |
