Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073015.t1 | XP_003603541.2 | 90.3 | 124 | 12 | 0 | 1 | 124 | 1 | 124 | 3.80E-54 | 220.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073015.t1 | G7JCD9 | 90.3 | 124 | 12 | 0 | 1 | 124 | 23 | 146 | 2.7e-54 | 220.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene073015 | -0.810775 | 9.74E-51 | -1.69E-46 |
| MS.gene049283 | MS.gene073015 | -0.814126 | 1.81E-51 | -1.69E-46 |
| MS.gene049419 | MS.gene073015 | 0.843097 | 1.73E-58 | -1.69E-46 |
| MS.gene049983 | MS.gene073015 | -0.812483 | 4.14E-51 | -1.69E-46 |
| MS.gene050105 | MS.gene073015 | -0.861082 | 1.32E-63 | -1.69E-46 |
| MS.gene051122 | MS.gene073015 | -0.811721 | 6.08E-51 | -1.69E-46 |
| MS.gene051332 | MS.gene073015 | -0.814803 | 1.28E-51 | -1.69E-46 |
| MS.gene051363 | MS.gene073015 | -0.801089 | 1.06E-48 | -1.69E-46 |
| MS.gene051368 | MS.gene073015 | -0.817502 | 3.19E-52 | -1.69E-46 |
| MS.gene051924 | MS.gene073015 | 0.819575 | 1.08E-52 | -1.69E-46 |
| MS.gene051926 | MS.gene073015 | 0.813976 | 1.95E-51 | -1.69E-46 |
| MS.gene05216 | MS.gene073015 | -0.819223 | 1.30E-52 | -1.69E-46 |
| MS.gene052333 | MS.gene073015 | -0.828459 | 8.89E-55 | -1.69E-46 |
| MS.gene052534 | MS.gene073015 | -0.807996 | 3.85E-50 | -1.69E-46 |
| MS.gene052586 | MS.gene073015 | -0.82496 | 6.08E-54 | -1.69E-46 |
| MS.gene052674 | MS.gene073015 | -0.820825 | 5.59E-53 | -1.69E-46 |
| MS.gene052675 | MS.gene073015 | -0.822874 | 1.88E-53 | -1.69E-46 |
| MS.gene052677 | MS.gene073015 | -0.822641 | 2.13E-53 | -1.69E-46 |
| MS.gene052954 | MS.gene073015 | -0.809201 | 2.13E-50 | -1.69E-46 |
| MS.gene05308 | MS.gene073015 | -0.804488 | 2.11E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene073015 | -0.802482 | 5.49E-49 | -1.69E-46 |
| MS.gene05350 | MS.gene073015 | -0.800701 | 1.27E-48 | -1.69E-46 |
| MS.gene054291 | MS.gene073015 | 0.809896 | 1.51E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene073015 | -0.859467 | 4.05E-63 | -1.69E-46 |
| MS.gene055701 | MS.gene073015 | -0.831695 | 1.44E-55 | -1.69E-46 |
| MS.gene055841 | MS.gene073015 | -0.80055 | 1.37E-48 | -1.69E-46 |
| MS.gene055926 | MS.gene073015 | -0.812336 | 4.46E-51 | -1.69E-46 |
| MS.gene056045 | MS.gene073015 | -0.830747 | 2.47E-55 | -1.69E-46 |
| MS.gene056480 | MS.gene073015 | -0.808803 | 2.59E-50 | -1.69E-46 |
| MS.gene05659 | MS.gene073015 | -0.801544 | 8.57E-49 | -1.69E-46 |
| MS.gene056776 | MS.gene073015 | -0.806384 | 8.44E-50 | -1.69E-46 |
| MS.gene057026 | MS.gene073015 | -0.805035 | 1.62E-49 | -1.69E-46 |
| MS.gene057155 | MS.gene073015 | -0.802584 | 5.23E-49 | -1.69E-46 |
| MS.gene057156 | MS.gene073015 | 0.832371 | 9.82E-56 | -1.69E-46 |
| MS.gene057208 | MS.gene073015 | -0.838928 | 2.15E-57 | -1.69E-46 |
| MS.gene057855 | MS.gene073015 | -0.820003 | 8.63E-53 | -1.69E-46 |
| MS.gene058081 | MS.gene073015 | -0.839395 | 1.63E-57 | -1.69E-46 |
| MS.gene058223 | MS.gene073015 | 0.855793 | 4.97E-62 | -1.69E-46 |
| MS.gene059283 | MS.gene073015 | -0.805042 | 1.62E-49 | -1.69E-46 |
| MS.gene059438 | MS.gene073015 | -0.820095 | 8.22E-53 | -1.69E-46 |
| MS.gene059439 | MS.gene073015 | -0.82318 | 1.59E-53 | -1.69E-46 |
| MS.gene059440 | MS.gene073015 | -0.808137 | 3.59E-50 | -1.69E-46 |
| MS.gene060435 | MS.gene073015 | -0.818967 | 1.49E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene073015 | -0.810895 | 9.18E-51 | -1.69E-46 |
| MS.gene060850 | MS.gene073015 | -0.808207 | 3.47E-50 | -1.69E-46 |
| MS.gene060851 | MS.gene073015 | -0.821561 | 3.78E-53 | -1.69E-46 |
| MS.gene060852 | MS.gene073015 | -0.819362 | 1.21E-52 | -1.69E-46 |
| MS.gene060932 | MS.gene073015 | -0.80459 | 2.01E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene073015 | -0.80974 | 1.63E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene073015 | -0.811064 | 8.44E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073015.t1 | MTR_3g108900 | 90.323 | 124 | 12 | 0 | 1 | 124 | 23 | 146 | 4.72e-71 | 211 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073015.t1 | AT5G10700 | 49.580 | 119 | 43 | 3 | 6 | 124 | 24 | 125 | 5.16e-33 | 114 |
| MS.gene073015.t1 | AT5G10700 | 67.901 | 81 | 23 | 2 | 45 | 124 | 47 | 125 | 5.91e-33 | 114 |
Find 38 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTTGACTCATTGAGTTAA+AGG | 0.322492 | 3.4:+94315696 | None:intergenic |
| CTGATCGATACTTGGCCATT+AGG | 0.378184 | 3.4:-94315583 | MS.gene073015:CDS |
| ACGCTTCTGTTTCCGCGGTT+TGG | 0.395935 | 3.4:-94315537 | MS.gene073015:CDS |
| AGTGGTGGAATCGGTGGAGG+CGG | 0.421097 | 3.4:+94315675 | None:intergenic |
| TGAGTAACCACACTGCCTAA+TGG | 0.453176 | 3.4:+94315568 | None:intergenic |
| GGTGACAGGATCGTCTTTGT+TGG | 0.456796 | 3.4:+94315513 | None:intergenic |
| TGGTCCAATACGTGGTGCTC+CGG | 0.468634 | 3.4:-94315612 | MS.gene073015:CDS |
| GAGAGTGGTGGAATCGGTGG+AGG | 0.477145 | 3.4:+94315672 | None:intergenic |
| TAAAGGTGAGCTGACTCGTC+CGG | 0.477990 | 3.4:+94315713 | None:intergenic |
| CTCCACATCAACTGATTCGC+CGG | 0.486687 | 3.4:-94315650 | MS.gene073015:CDS |
| CACGCCGGAGCACCACGTAT+TGG | 0.503895 | 3.4:+94315608 | None:intergenic |
| GGCGTGATCTGATCGATACT+TGG | 0.507820 | 3.4:-94315591 | MS.gene073015:CDS |
| CTACAATAATCAACGGTGAC+AGG | 0.516772 | 3.4:+94315499 | None:intergenic |
| TGAGAAGGCGAGGTACGAAC+GGG | 0.519329 | 3.4:+94315780 | None:intergenic |
| GTGGTGGAATCGGTGGAGGC+GGG | 0.520690 | 3.4:+94315676 | None:intergenic |
| TTGAGAAGGCGAGGTACGAA+CGG | 0.522363 | 3.4:+94315779 | None:intergenic |
| TGTGGAGAGAGTGGTGGAAT+CGG | 0.534342 | 3.4:+94315666 | None:intergenic |
| AGGCGAGGTACGAACGGGAG+GGG | 0.553596 | 3.4:+94315785 | None:intergenic |
| TTCCGGCGAATCAGTTGATG+TGG | 0.555932 | 3.4:+94315648 | None:intergenic |
| GGGTTGAAGAGACGAAGCGT+AGG | 0.561056 | 3.4:+94315805 | None:intergenic |
| TTAGGCAGTGTGGTTACTCA+AGG | 0.562328 | 3.4:-94315565 | MS.gene073015:CDS |
| GAAGGCGAGGTACGAACGGG+AGG | 0.562660 | 3.4:+94315783 | None:intergenic |
| GGAGAGAGTGGTGGAATCGG+TGG | 0.566146 | 3.4:+94315669 | None:intergenic |
| AAGGCGAGGTACGAACGGGA+GGG | 0.566860 | 3.4:+94315784 | None:intergenic |
| GCCGGAAAACGTCGATGTTG+TGG | 0.569743 | 3.4:-94315632 | MS.gene073015:CDS |
| TGAAGAGACGAAGCGTAGGA+AGG | 0.573707 | 3.4:+94315809 | None:intergenic |
| ACTAAACTTCGTTCTTGAGA+AGG | 0.577942 | 3.4:+94315765 | None:intergenic |
| GAAGAGACGAAGCGTAGGAA+GGG | 0.601157 | 3.4:+94315810 | None:intergenic |
| GTCGGGGCTACAATAATCAA+CGG | 0.604557 | 3.4:+94315492 | None:intergenic |
| ACTTCGTTCTTGAGAAGGCG+AGG | 0.622634 | 3.4:+94315770 | None:intergenic |
| TTGTCACGCTTCTGTTTCCG+CGG | 0.641384 | 3.4:-94315542 | MS.gene073015:CDS |
| ATCAGTTGATGTGGAGAGAG+TGG | 0.646257 | 3.4:+94315657 | None:intergenic |
| CGATGTTGTGGTCCAATACG+TGG | 0.652164 | 3.4:-94315620 | MS.gene073015:CDS |
| GTCTTTGTTGGACCAAACCG+CGG | 0.653891 | 3.4:+94315525 | None:intergenic |
| AGTTGATGTGGAGAGAGTGG+TGG | 0.659985 | 3.4:+94315660 | None:intergenic |
| TACTTGGCCATTAGGCAGTG+TGG | 0.667910 | 3.4:-94315575 | MS.gene073015:CDS |
| GTTTAGTGTCACTTTACACG+CGG | 0.717247 | 3.4:-94315749 | MS.gene073015:CDS |
| AAGTATCGATCAGATCACGC+CGG | 0.740521 | 3.4:+94315593 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTGCATAGAATCAATTTTGT+CGG | + | chr3.4:94315820-94315839 | None:intergenic | 30.0% |
| ! | TGCATAGAATCAATTTTGTC+GGG | + | chr3.4:94315819-94315838 | None:intergenic | 30.0% |
| ACTAAACTTCGTTCTTGAGA+AGG | + | chr3.4:94315529-94315548 | None:intergenic | 35.0% | |
| ! | GCATAGAATCAATTTTGTCG+GGG | + | chr3.4:94315818-94315837 | None:intergenic | 35.0% |
| CTACAATAATCAACGGTGAC+AGG | + | chr3.4:94315795-94315814 | None:intergenic | 40.0% | |
| ! | GGGTTGACTCATTGAGTTAA+AGG | + | chr3.4:94315598-94315617 | None:intergenic | 40.0% |
| !! | GTTTAGTGTCACTTTACACG+CGG | - | chr3.4:94315542-94315561 | MS.gene073015:CDS | 40.0% |
| CTGATCGATACTTGGCCATT+AGG | - | chr3.4:94315708-94315727 | MS.gene073015:CDS | 45.0% | |
| GTCGGGGCTACAATAATCAA+CGG | + | chr3.4:94315802-94315821 | None:intergenic | 45.0% | |
| TGAGTAACCACACTGCCTAA+TGG | + | chr3.4:94315726-94315745 | None:intergenic | 45.0% | |
| TTAGGCAGTGTGGTTACTCA+AGG | - | chr3.4:94315726-94315745 | MS.gene073015:CDS | 45.0% | |
| ! | ACCACAACATCGACGTTTTC+CGG | + | chr3.4:94315663-94315682 | None:intergenic | 45.0% |
| ! | ACTCGTCCGGTTTGAAAAAG+AGG | + | chr3.4:94315568-94315587 | None:intergenic | 45.0% |
| ! | ATCAGTTGATGTGGAGAGAG+TGG | + | chr3.4:94315637-94315656 | None:intergenic | 45.0% |
| !! | AAGTATCGATCAGATCACGC+CGG | + | chr3.4:94315701-94315720 | None:intergenic | 45.0% |
| ACTTCGTTCTTGAGAAGGCG+AGG | + | chr3.4:94315524-94315543 | None:intergenic | 50.0% | |
| CGATGTTGTGGTCCAATACG+TGG | - | chr3.4:94315671-94315690 | MS.gene073015:CDS | 50.0% | |
| GAAGAGACGAAGCGTAGGAA+GGG | + | chr3.4:94315484-94315503 | None:intergenic | 50.0% | |
| GGCGTGATCTGATCGATACT+TGG | - | chr3.4:94315700-94315719 | MS.gene073015:CDS | 50.0% | |
| GGTGACAGGATCGTCTTTGT+TGG | + | chr3.4:94315781-94315800 | None:intergenic | 50.0% | |
| GTCTTTGTTGGACCAAACCG+CGG | + | chr3.4:94315769-94315788 | None:intergenic | 50.0% | |
| TACTTGGCCATTAGGCAGTG+TGG | - | chr3.4:94315716-94315735 | MS.gene073015:CDS | 50.0% | |
| TGAAGAGACGAAGCGTAGGA+AGG | + | chr3.4:94315485-94315504 | None:intergenic | 50.0% | |
| TTGAGAAGGCGAGGTACGAA+CGG | + | chr3.4:94315515-94315534 | None:intergenic | 50.0% | |
| TTGTCACGCTTCTGTTTCCG+CGG | - | chr3.4:94315749-94315768 | MS.gene073015:CDS | 50.0% | |
| ! | AGTTGATGTGGAGAGAGTGG+TGG | + | chr3.4:94315634-94315653 | None:intergenic | 50.0% |
| ! | TGTGGAGAGAGTGGTGGAAT+CGG | + | chr3.4:94315628-94315647 | None:intergenic | 50.0% |
| ! | TTCCGGCGAATCAGTTGATG+TGG | + | chr3.4:94315646-94315665 | None:intergenic | 50.0% |
| !! | CTCCACATCAACTGATTCGC+CGG | - | chr3.4:94315641-94315660 | MS.gene073015:CDS | 50.0% |
| !! | TAAAGGTGAGCTGACTCGTC+CGG | + | chr3.4:94315581-94315600 | None:intergenic | 50.0% |
| !!! | ACGCGGCCTCTTTTTCAAAC+CGG | - | chr3.4:94315559-94315578 | MS.gene073015:CDS | 50.0% |
| ACGCTTCTGTTTCCGCGGTT+TGG | - | chr3.4:94315754-94315773 | MS.gene073015:CDS | 55.0% | |
| GCCGGAAAACGTCGATGTTG+TGG | - | chr3.4:94315659-94315678 | MS.gene073015:CDS | 55.0% | |
| GGGTTGAAGAGACGAAGCGT+AGG | + | chr3.4:94315489-94315508 | None:intergenic | 55.0% | |
| TGAGAAGGCGAGGTACGAAC+GGG | + | chr3.4:94315514-94315533 | None:intergenic | 55.0% | |
| TGGTCCAATACGTGGTGCTC+CGG | - | chr3.4:94315679-94315698 | MS.gene073015:CDS | 55.0% | |
| AAGGCGAGGTACGAACGGGA+GGG | + | chr3.4:94315510-94315529 | None:intergenic | 60.0% | |
| ! | AGTGGTGGAATCGGTGGAGG+CGG | + | chr3.4:94315619-94315638 | None:intergenic | 60.0% |
| ! | GAGAGTGGTGGAATCGGTGG+AGG | + | chr3.4:94315622-94315641 | None:intergenic | 60.0% |
| ! | GGAGAGAGTGGTGGAATCGG+TGG | + | chr3.4:94315625-94315644 | None:intergenic | 60.0% |
| AGGCGAGGTACGAACGGGAG+GGG | + | chr3.4:94315509-94315528 | None:intergenic | 65.0% | |
| GAAGGCGAGGTACGAACGGG+AGG | + | chr3.4:94315511-94315530 | None:intergenic | 65.0% | |
| ! | CACGCCGGAGCACCACGTAT+TGG | + | chr3.4:94315686-94315705 | None:intergenic | 65.0% |
| ! | GTGGTGGAATCGGTGGAGGC+GGG | + | chr3.4:94315618-94315637 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 94315471 | 94315842 | 94315471 | ID=MS.gene073015 |
| chr3.4 | mRNA | 94315471 | 94315842 | 94315471 | ID=MS.gene073015.t1;Parent=MS.gene073015 |
| chr3.4 | exon | 94315471 | 94315842 | 94315471 | ID=MS.gene073015.t1.exon1;Parent=MS.gene073015.t1 |
| chr3.4 | CDS | 94315471 | 94315842 | 94315471 | ID=cds.MS.gene073015.t1;Parent=MS.gene073015.t1 |
| Gene Sequence |
| Protein sequence |
