AlfalfaGEDB | MS.gene21659

M. sativa cultivar XinJiangDaYe / MS.gene21659

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21659.t1	XP_003611004.1	97.8	182	4	0	1	182	139	320	6.70E-68	266.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21659.t1	Q9C7W2	59.6	188	58	4	1	181	158	334	4.3e-31	136.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21659.t1	G7KAL6	97.8	182	4	0	1	182	139	320	4.9e-68	266.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene21659.t1	TF	AP2/ERF-ERF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049297	MS.gene21659	0.814422	1.55E-51	-1.69E-46
MS.gene049378	MS.gene21659	0.805891	1.07E-49	-1.69E-46
MS.gene050226	MS.gene21659	0.809256	2.07E-50	-1.69E-46
MS.gene050227	MS.gene21659	0.8123	4.54E-51	-1.69E-46
MS.gene050228	MS.gene21659	0.820123	8.10E-53	-1.69E-46
MS.gene050277	MS.gene21659	0.851192	1.04E-60	-1.69E-46
MS.gene050623	MS.gene21659	-0.834294	3.26E-56	-1.69E-46
MS.gene050987	MS.gene21659	0.82624	3.02E-54	-1.69E-46
MS.gene051245	MS.gene21659	0.818927	1.52E-52	-1.69E-46
MS.gene051633	MS.gene21659	0.82919	5.91E-55	-1.69E-46
MS.gene051662	MS.gene21659	0.802211	6.25E-49	-1.69E-46
MS.gene051903	MS.gene21659	0.802005	6.89E-49	-1.69E-46
MS.gene052176	MS.gene21659	0.831351	1.75E-55	-1.69E-46
MS.gene052286	MS.gene21659	0.854553	1.14E-61	-1.69E-46
MS.gene052302	MS.gene21659	0.869211	3.67E-66	-1.69E-46
MS.gene052309	MS.gene21659	-0.829929	3.91E-55	-1.69E-46
MS.gene052376	MS.gene21659	0.860512	1.96E-63	-1.69E-46
MS.gene052856	MS.gene21659	0.813295	2.75E-51	-1.69E-46
MS.gene052858	MS.gene21659	0.806602	7.60E-50	-1.69E-46
MS.gene052860	MS.gene21659	0.809995	1.44E-50	-1.69E-46
MS.gene053189	MS.gene21659	0.806644	7.44E-50	-1.69E-46
MS.gene053216	MS.gene21659	0.805543	1.27E-49	-1.69E-46
MS.gene054536	MS.gene21659	0.806303	8.78E-50	-1.69E-46
MS.gene054537	MS.gene21659	0.80753	4.83E-50	-1.69E-46
MS.gene054930	MS.gene21659	0.811964	5.38E-51	-1.69E-46
MS.gene054942	MS.gene21659	0.812669	3.77E-51	-1.69E-46
MS.gene055133	MS.gene21659	0.802047	6.75E-49	-1.69E-46
MS.gene055207	MS.gene21659	0.801243	9.87E-49	-1.69E-46
MS.gene055441	MS.gene21659	0.827288	1.70E-54	-1.69E-46
MS.gene056069	MS.gene21659	-0.812197	4.78E-51	-1.69E-46
MS.gene056631	MS.gene21659	0.827756	1.31E-54	-1.69E-46
MS.gene056760	MS.gene21659	0.805986	1.02E-49	-1.69E-46
MS.gene057057	MS.gene21659	0.804137	2.50E-49	-1.69E-46
MS.gene057079	MS.gene21659	0.828213	1.02E-54	-1.69E-46
MS.gene057234	MS.gene21659	0.81022	1.28E-50	-1.69E-46
MS.gene057451	MS.gene21659	-0.82033	7.26E-53	-1.69E-46
MS.gene057452	MS.gene21659	-0.808268	3.37E-50	-1.69E-46
MS.gene057754	MS.gene21659	0.812631	3.85E-51	-1.69E-46
MS.gene05795	MS.gene21659	0.820622	6.22E-53	-1.69E-46
MS.gene058182	MS.gene21659	0.822359	2.47E-53	-1.69E-46
MS.gene058201	MS.gene21659	0.80621	9.19E-50	-1.69E-46
MS.gene058238	MS.gene21659	0.827561	1.46E-54	-1.69E-46
MS.gene058661	MS.gene21659	-0.821441	4.03E-53	-1.69E-46
MS.gene058906	MS.gene21659	0.825182	5.39E-54	-1.69E-46
MS.gene059872	MS.gene21659	-0.802585	5.23E-49	-1.69E-46
MS.gene059899	MS.gene21659	0.806268	8.93E-50	-1.69E-46
MS.gene06001	MS.gene21659	0.820678	6.04E-53	-1.69E-46
MS.gene060297	MS.gene21659	0.854099	1.54E-61	-1.69E-46
MS.gene060476	MS.gene21659	0.80355	3.31E-49	-1.69E-46
MS.gene061113	MS.gene21659	0.814113	1.82E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21659.t1	MTR_5g009410	98.361	183	1	2	1	182	139	320	1.25e-127	361
MS.gene21659.t1	MTR_3g074130	42.958	142	60	3	2	124	173	312	2.57e-24	97.4
MS.gene21659.t1	MTR_5g062700	57.971	69	25	2	2	69	196	261	1.41e-20	87.8
MS.gene21659.t1	MTR_8g090350	55.882	68	22	2	2	69	136	195	1.47e-18	81.3
MS.gene21659.t1	MTR_1g090170	35.165	182	74	6	2	182	262	400	1.78e-18	82.0
MS.gene21659.t1	MTR_1g090170	35.165	182	74	6	2	182	175	313	1.82e-18	81.3
MS.gene21659.t1	MTR_8g006840	65.306	49	17	0	2	50	149	197	4.19e-13	65.1
MS.gene21659.t1	MTR_8g006815	73.684	38	10	0	2	39	120	157	8.83e-13	63.9
MS.gene21659.t1	MTR_8g006825	62.222	45	14	1	2	43	120	164	3.70e-12	62.8
MS.gene21659.t1	MTR_2g090235	75.000	36	9	0	2	37	241	276	4.81e-12	63.9
MS.gene21659.t1	MTR_1g014800	37.895	95	56	1	2	96	54	145	6.14e-12	62.0
MS.gene21659.t1	MTR_7g081795	66.667	36	12	0	2	37	84	119	2.92e-11	60.1
MS.gene21659.t1	MTR_4g100380	76.471	34	8	0	2	35	169	202	6.32e-11	60.1
MS.gene21659.t1	MTR_2g015040	73.529	34	9	0	2	35	87	120	6.84e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21659.t1	AT1G64380	52.128	188	72	6	1	181	158	334	7.39e-33	120
MS.gene21659.t1	AT1G78080	43.448	145	67	5	2	132	175	318	1.88e-24	97.8
MS.gene21659.t1	AT4G39780	55.714	70	30	1	2	71	116	184	1.43e-20	86.3
MS.gene21659.t1	AT1G22190	50.000	76	30	1	2	77	106	173	2.20e-19	83.2
MS.gene21659.t1	AT2G22200	51.429	70	25	1	2	71	94	154	1.92e-17	77.8
MS.gene21659.t1	AT1G36060	50.000	74	37	0	2	75	166	239	3.93e-17	77.8
MS.gene21659.t1	AT4G13620	30.000	180	75	4	2	181	255	383	3.08e-14	70.1
MS.gene21659.t1	AT5G65130	49.231	65	26	2	2	66	134	191	4.50e-13	66.2
MS.gene21659.t1	AT5G65130	49.231	65	26	2	2	66	136	193	4.81e-13	65.9
MS.gene21659.t1	AT2G44840	75.000	36	9	0	2	37	116	151	5.66e-12	62.4
MS.gene21659.t1	AT2G20880	75.000	36	9	0	2	37	210	245	8.75e-12	62.8

Find 27 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAGTTGGATCTTTCAAATT+TGG	0.137255	5.2:-3071598	None:intergenic
TTGGGAAGTTCTTGCTGTTT+AGG	0.207034	5.2:+3072023	MS.gene21659:CDS
CTGTTTCTGAGGATGGAATT+TGG	0.226527	5.2:+3071896	MS.gene21659:CDS
GATCCAACTAAGTTGGGTTT+TGG	0.259771	5.2:+3071610	MS.gene21659:CDS
TGGGAAGTTCTTGCTGTTTA+GGG	0.276754	5.2:+3072024	MS.gene21659:CDS
CACAGAATAGAATGAGAGTT+TGG	0.304146	5.2:+3071485	None:intergenic
ACCATTGTCGAAACCGAATT+CGG	0.358811	5.2:-3071934	None:intergenic
AGAACTTCCCAAATCAATTC+AGG	0.364637	5.2:-3072012	None:intergenic
AAGAAGATGAAGAAGAATTC+AGG	0.374931	5.2:+3071736	MS.gene21659:CDS
TTTGAAAGATCCAACTAAGT+TGG	0.380517	5.2:+3071603	MS.gene21659:CDS
AGGGGAGAATTCTCCGAATT+CGG	0.382016	5.2:+3071921	MS.gene21659:CDS
TTGAAAGATCCAACTAAGTT+GGG	0.405515	5.2:+3071604	MS.gene21659:CDS
CTGAGGATGGAATTTGGAAA+GGG	0.444826	5.2:+3071902	MS.gene21659:CDS
CTATTTGCCAAAAGGTGAAA+AGG	0.446134	5.2:+3071686	MS.gene21659:CDS
TCTCCAAAACCCAACTTAGT+TGG	0.464313	5.2:-3071613	None:intergenic
TCACCAACTGTTTCTGAGGA+TGG	0.477469	5.2:+3071889	MS.gene21659:CDS
TCCGAATTCGGTTTCGACAA+TGG	0.480538	5.2:+3071933	MS.gene21659:CDS
AATTCAAGCTATTTGCCAAA+AGG	0.481211	5.2:+3071678	MS.gene21659:CDS
ATCAATTCAGGATCAAAAGA+TGG	0.507401	5.2:-3072000	None:intergenic
TATTTGCCAAAAGGTGAAAA+GGG	0.519794	5.2:+3071687	MS.gene21659:CDS
CGTGCAGCTTATAAGCTTCG+AGG	0.521878	5.2:+3071553	MS.gene21659:CDS
AAAAGATGGCATTCTTGCTA+AGG	0.531887	5.2:-3071986	None:intergenic
TCTGAGGATGGAATTTGGAA+AGG	0.539276	5.2:+3071901	MS.gene21659:CDS
TGAGGATGGAATTTGGAAAG+GGG	0.545281	5.2:+3071903	MS.gene21659:CDS
AATAGAATGAGAGTTTGGCT+TGG	0.577666	5.2:+3071490	None:intergenic
ATTCCATCCTCAGAAACAGT+TGG	0.628763	5.2:-3071892	None:intergenic
GGTTTCGACAATGGTGACAG+AGG	0.648753	5.2:+3071942	MS.gene21659:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTAGTTGGATCTTTCAAATT+TGG	-	chr5.2:3071601-3071620	None:intergenic	25.0%
!!!	TCTTGTTTTTTGTGATGATT+GGG	+	chr5.2:3071852-3071871	MS.gene21659:CDS	25.0%
!!!	TTTTGATCCTGAATTGATTT+GGG	+	chr5.2:3072005-3072024	MS.gene21659:CDS	25.0%
	AAGAAGATGAAGAAGAATTC+AGG	+	chr5.2:3071736-3071755	MS.gene21659:CDS	30.0%
	AATTCAAGCTATTTGCCAAA+AGG	+	chr5.2:3071678-3071697	MS.gene21659:CDS	30.0%
	ATCAATTCAGGATCAAAAGA+TGG	-	chr5.2:3072003-3072022	None:intergenic	30.0%
	CAATCATCACAAAAAACAAG+AGG	-	chr5.2:3071853-3071872	None:intergenic	30.0%
	TATTTGCCAAAAGGTGAAAA+GGG	+	chr5.2:3071687-3071706	MS.gene21659:CDS	30.0%
	TTGAAAGATCCAACTAAGTT+GGG	+	chr5.2:3071604-3071623	MS.gene21659:CDS	30.0%
	TTTGAAAGATCCAACTAAGT+TGG	+	chr5.2:3071603-3071622	MS.gene21659:CDS	30.0%
!	CTTTTGATCCTGAATTGATT+TGG	+	chr5.2:3072004-3072023	MS.gene21659:CDS	30.0%
!!!	CTCTTGTTTTTTGTGATGAT+TGG	+	chr5.2:3071851-3071870	MS.gene21659:CDS	30.0%
	AAAAGATGGCATTCTTGCTA+AGG	-	chr5.2:3071989-3072008	None:intergenic	35.0%
	AGAACTTCCCAAATCAATTC+AGG	-	chr5.2:3072015-3072034	None:intergenic	35.0%
	CTATTTGCCAAAAGGTGAAA+AGG	+	chr5.2:3071686-3071705	MS.gene21659:CDS	35.0%
!	CTTTTTCCCTTTTCACCTTT+TGG	-	chr5.2:3071696-3071715	None:intergenic	35.0%
	ACCATTGTCGAAACCGAATT+CGG	-	chr5.2:3071937-3071956	None:intergenic	40.0%
	ATTCCATCCTCAGAAACAGT+TGG	-	chr5.2:3071895-3071914	None:intergenic	40.0%
	CAAAAAACAAGAGGCGAAAG+TGG	-	chr5.2:3071844-3071863	None:intergenic	40.0%
	CTGAGGATGGAATTTGGAAA+GGG	+	chr5.2:3071902-3071921	MS.gene21659:CDS	40.0%
	TCTCCAAAACCCAACTTAGT+TGG	-	chr5.2:3071616-3071635	None:intergenic	40.0%
	TCTGAGGATGGAATTTGGAA+AGG	+	chr5.2:3071901-3071920	MS.gene21659:CDS	40.0%
	TGAGGATGGAATTTGGAAAG+GGG	+	chr5.2:3071903-3071922	MS.gene21659:CDS	40.0%
!!	CTGTTTCTGAGGATGGAATT+TGG	+	chr5.2:3071896-3071915	MS.gene21659:CDS	40.0%
!!	GATCCAACTAAGTTGGGTTT+TGG	+	chr5.2:3071610-3071629	MS.gene21659:CDS	40.0%
!!!	GTTTTCACCAACTGTTTCTG+AGG	+	chr5.2:3071885-3071904	MS.gene21659:CDS	40.0%
	AGGGGAGAATTCTCCGAATT+CGG	+	chr5.2:3071921-3071940	MS.gene21659:CDS	45.0%
	TCCGAATTCGGTTTCGACAA+TGG	+	chr5.2:3071933-3071952	MS.gene21659:CDS	45.0%
!!	TCACCAACTGTTTCTGAGGA+TGG	+	chr5.2:3071889-3071908	MS.gene21659:CDS	45.0%
	CGTGCAGCTTATAAGCTTCG+AGG	+	chr5.2:3071553-3071572	MS.gene21659:CDS	50.0%
	GGTTTCGACAATGGTGACAG+AGG	+	chr5.2:3071942-3071961	MS.gene21659:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	3071496	3072044	3071496	ID=MS.gene21659
chr5.2	mRNA	3071496	3072044	3071496	ID=MS.gene21659.t1;Parent=MS.gene21659
chr5.2	exon	3071496	3072044	3071496	ID=MS.gene21659.t1.exon1;Parent=MS.gene21659.t1
chr5.2	CDS	3071496	3072044	3071496	ID=cds.MS.gene21659.t1;Parent=MS.gene21659.t1

Gene Sequence

>MS.gene21659

ATGAGAGTTTGGCTTGGAACTTATGAAACTGCTGAAGCTGCAGCTTATGCTTATGATCGTGCAGCTTATAAGCTTCGAGGCGAATACGCGCGTTTAAATTTTCCAAATTTGAAAGATCCAACTAAGTTGGGTTTTGGAGATTCTACTAGATTGAATGCTTTGAAGAATTCTGTTGATGCTAAAATTCAAGCTATTTGCCAAAAGGTGAAAAGGGAAAAAGCTAAAAAGATTGCTGCTGCAAAGAAGATGAAGAAGAATTCAGGTAGTAGTAATGATACAAAGAGTGATAAAAATTCAGACAAGATTATTAATTCATCTTCTTGTTCTTCTTCTTCGTCTTCGTTACCACTTTCGCCTCTTGTTTTTTGTGATGATTGGGTGAATGAATTGTTTTCACCAACTGTTTCTGAGGATGGAATTTGGAAAGGGGAGAATTCTCCGAATTCGGTTTCGACAATGGTGACAGAGGAAACAGAGTTTGAAGATTGTTCCTTAGCAAGAATGCCATCTTTTGATCCTGAATTGATTTGGGAAGTTCTTGCTGTTTAG

Protein sequence

>MS.gene21659.t1

MRVWLGTYETAEAAAYAYDRAAYKLRGEYARLNFPNLKDPTKLGFGDSTRLNALKNSVDAKIQAICQKVKREKAKKIAAAKKMKKNSGSSNDTKSDKNSDKIINSSSCSSSSSSLPLSPLVFCDDWVNELFSPTVSEDGIWKGENSPNSVSTMVTEETEFEDCSLARMPSFDPELIWEVLAV