Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23466.t1 | XP_013449911.1 | 100 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 3.70E-29 | 136.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23466.t1 | Q93XX8 | 79.7 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 2.6e-23 | 108.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23466.t1 | A0A072U2M3 | 100.0 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 2.7e-29 | 136.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene31515 | MS.gene23466 | PPI |
| MS.gene059422 | MS.gene23466 | PPI |
| MS.gene08205 | MS.gene23466 | PPI |
| MS.gene057730 | MS.gene23466 | PPI |
| MS.gene90954 | MS.gene23466 | PPI |
| MS.gene23466 | MS.gene98436 | PPI |
| MS.gene41916 | MS.gene23466 | PPI |
| MS.gene037324 | MS.gene23466 | PPI |
| MS.gene51868 | MS.gene23466 | PPI |
| MS.gene000714 | MS.gene23466 | PPI |
| MS.gene003501 | MS.gene23466 | PPI |
| MS.gene23466 | MS.gene72894 | PPI |
| MS.gene99744 | MS.gene23466 | PPI |
| MS.gene23466 | MS.gene001986 | PPI |
| MS.gene029242 | MS.gene23466 | PPI |
| MS.gene028023 | MS.gene23466 | PPI |
| MS.gene68979 | MS.gene23466 | PPI |
| MS.gene038800 | MS.gene23466 | PPI |
| MS.gene046040 | MS.gene23466 | PPI |
| MS.gene23466 | MS.gene054186 | PPI |
| MS.gene064180 | MS.gene23466 | PPI |
| MS.gene90957 | MS.gene23466 | PPI |
| MS.gene45344 | MS.gene23466 | PPI |
| MS.gene23466 | MS.gene044498 | PPI |
| MS.gene23466 | MS.gene033017 | PPI |
| MS.gene23466 | MS.gene067809 | PPI |
| MS.gene056257 | MS.gene23466 | PPI |
| MS.gene23466 | MS.gene52097 | PPI |
| MS.gene95758 | MS.gene23466 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23466.t1 | MTR_7g097110 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 2.26e-43 | 134 |
| MS.gene23466.t1 | MTR_8g069770 | 85.938 | 64 | 9 | 0 | 1 | 64 | 1 | 64 | 7.70e-36 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23466.t1 | AT2G20490 | 79.688 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 8.91e-33 | 107 |
| MS.gene23466.t1 | AT2G20490 | 79.688 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 8.91e-33 | 107 |
| MS.gene23466.t1 | AT2G20490 | 78.125 | 64 | 13 | 1 | 1 | 64 | 1 | 63 | 2.46e-30 | 101 |
Find 17 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATTCAAGGTTTACCTTAG+TGG | 0.314345 | 7.3:-14241729 | None:intergenic |
| AATACTTTGGAGCTGGATGC+TGG | 0.333958 | 7.3:-14243332 | None:intergenic |
| GTTAAACTGAAGAAGCGATT+TGG | 0.348976 | 7.3:+14243297 | MS.gene23466:CDS |
| TGCTCTGAAAATTTGTCATC+AGG | 0.402146 | 7.3:-14243270 | None:intergenic |
| ATTTGTCATCAGGTGAAAAC+CGG | 0.402459 | 7.3:-14243260 | None:intergenic |
| TTGAATATAGCTCAATACTT+TGG | 0.457998 | 7.3:-14243345 | None:intergenic |
| TGCAGAAAGAGTCACCGCTT+GGG | 0.506313 | 7.3:+14241850 | MS.gene23466:intron |
| ATAGCTCAATACTTTGGAGC+TGG | 0.511390 | 7.3:-14243339 | None:intergenic |
| TTGCAGAAAGAGTCACCGCT+TGG | 0.526675 | 7.3:+14241849 | MS.gene23466:intron |
| GCTACTCAATCTGCCCATCC+AGG | 0.527175 | 7.3:+14241876 | MS.gene23466:CDS |
| TTTGGAGCTGGATGCTGGGT+CGG | 0.555768 | 7.3:-14243327 | None:intergenic |
| ATACTTTGGAGCTGGATGCT+GGG | 0.560513 | 7.3:-14243331 | None:intergenic |
| TTTGTCATCAGGTGAAAACC+GGG | 0.561589 | 7.3:-14243259 | None:intergenic |
| CATTAACGATAACGGTGACA+AGG | 0.605553 | 7.3:+14241700 | MS.gene23466:CDS |
| AATGTAAAACTGAAGATACA+TGG | 0.612653 | 7.3:-14241681 | None:intergenic |
| TGACAAGGTCTACACCACTA+AGG | 0.622254 | 7.3:+14241715 | MS.gene23466:CDS |
| GATTGAGTAGCTAACCCAAG+CGG | 0.725473 | 7.3:-14241864 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATTTTTTTTTTTTAAAA+AGG | + | chr7.3:14242683-14242702 | MS.gene23466:intron | 0.0% |
| !! | TAAAAATATGATAACTAAAG+AGG | - | chr7.3:14242475-14242494 | None:intergenic | 15.0% |
| !!! | AAAAGTTTTCAAAACAAAAA+AGG | - | chr7.3:14243152-14243171 | None:intergenic | 15.0% |
| !!! | AAAGTTTTCAAAACAAAAAA+GGG | - | chr7.3:14243151-14243170 | None:intergenic | 15.0% |
| !!! | TTTTAACATTCACTTAAAAT+TGG | - | chr7.3:14242250-14242269 | None:intergenic | 15.0% |
| !! | AAAAAGTCATTAATCATTGT+AGG | - | chr7.3:14243217-14243236 | None:intergenic | 20.0% |
| !! | ATAAGAAGTCTATGTTAAAA+TGG | + | chr7.3:14242176-14242195 | MS.gene23466:intron | 20.0% |
| !! | TAAAGAAAAATGACTTGATT+TGG | + | chr7.3:14242945-14242964 | MS.gene23466:intron | 20.0% |
| !! | TAAGAAGTCTATGTTAAAAT+GGG | + | chr7.3:14242177-14242196 | MS.gene23466:intron | 20.0% |
| !!! | ATGACTTTTTCTTTTGTTTT+CGG | + | chr7.3:14243227-14243246 | MS.gene23466:intron | 20.0% |
| !!! | CAATATTTTACCTCAAAAAT+TGG | - | chr7.3:14242082-14242101 | None:intergenic | 20.0% |
| ! | AACTACAAAAGAATTAGATG+AGG | - | chr7.3:14242919-14242938 | None:intergenic | 25.0% |
| ! | AAGAAGTCTATGTTAAAATG+GGG | + | chr7.3:14242178-14242197 | MS.gene23466:intron | 25.0% |
| ! | ACTACAAAAGAATTAGATGA+GGG | - | chr7.3:14242918-14242937 | None:intergenic | 25.0% |
| ! | CATTAATCATTGTAGGAAAT+TGG | - | chr7.3:14243210-14243229 | None:intergenic | 25.0% |
| ! | GGTAAAATATTGAAACTGAT+AGG | + | chr7.3:14242090-14242109 | MS.gene23466:intron | 25.0% |
| ! | GTATTAAAATCCTTGATTCA+AGG | - | chr7.3:14241745-14241764 | None:intergenic | 25.0% |
| ! | TATGTGTCATGTCAAAATTT+AGG | + | chr7.3:14242369-14242388 | MS.gene23466:intron | 25.0% |
| ! | TCAAAGATTAAAGCACAAAA+TGG | - | chr7.3:14241932-14241951 | None:intergenic | 25.0% |
| !! | CAGTTTTACATTAACGATAA+CGG | + | chr7.3:14241692-14241711 | MS.gene23466:CDS | 25.0% |
| !! | GATTTTGTATTTACTGCAAT+TGG | + | chr7.3:14242210-14242229 | MS.gene23466:intron | 25.0% |
| !!! | AAATGACTTGATTTGGAATT+TGG | + | chr7.3:14242952-14242971 | MS.gene23466:intron | 25.0% |
| !!! | ATGTTTTGAATTGTGAAGTA+AGG | + | chr7.3:14243038-14243057 | MS.gene23466:intron | 25.0% |
| !!! | CCTAAGTTAAATTGTTTTAG+CGG | - | chr7.3:14242855-14242874 | None:intergenic | 25.0% |
| !!! | TGTTTTGAATTGTGAAGTAA+GGG | + | chr7.3:14243039-14243058 | MS.gene23466:intron | 25.0% |
| AAAAATTGGATGTGTGTCAT+TGG | - | chr7.3:14242068-14242087 | None:intergenic | 30.0% | |
| ACAAAATGGCAGTAAAACTA+TGG | - | chr7.3:14241918-14241937 | None:intergenic | 30.0% | |
| ACACTCTGATACATAAGTAT+TGG | - | chr7.3:14242712-14242731 | None:intergenic | 30.0% | |
| AGAAACTTTGGATTTGCAAT+CGG | - | chr7.3:14242583-14242602 | None:intergenic | 30.0% | |
| AGTGGATCATGAAAAAGAAT+AGG | - | chr7.3:14242396-14242415 | None:intergenic | 30.0% | |
| GCTATTAAAAAATGAGACCA+AGG | + | chr7.3:14243005-14243024 | MS.gene23466:intron | 30.0% | |
| GTATTTACTGCAATTGGATT+TGG | + | chr7.3:14242216-14242235 | MS.gene23466:intron | 30.0% | |
| GTGCTTTAATCTTTGATACA+TGG | + | chr7.3:14241936-14241955 | MS.gene23466:intron | 30.0% | |
| TTTGCAATGAGAATTAAGAG+TGG | - | chr7.3:14242439-14242458 | None:intergenic | 30.0% | |
| ! | ATCTGATGCATTTTCCTTAT+TGG | + | chr7.3:14242880-14242899 | MS.gene23466:intron | 30.0% |
| ! | CCGCTAAAACAATTTAACTT+AGG | + | chr7.3:14242852-14242871 | MS.gene23466:intron | 30.0% |
| ! | TCGTGGGTTGTAAATAATAT+TGG | - | chr7.3:14243186-14243205 | None:intergenic | 30.0% |
| ! | TCTGATGCATTTTCCTTATT+GGG | + | chr7.3:14242881-14242900 | MS.gene23466:intron | 30.0% |
| ! | TTTTAGCAAAATACCTGGAT+GGG | - | chr7.3:14241892-14241911 | None:intergenic | 30.0% |
| !! | ATTTTAGCAAAATACCTGGA+TGG | - | chr7.3:14241893-14241912 | None:intergenic | 30.0% |
| !! | GTGGTTTTGCAATTAGAATT+GGG | + | chr7.3:14242313-14242332 | MS.gene23466:intron | 30.0% |
| !!! | GTGATTTTGGTTACAACATT+AGG | + | chr7.3:14242791-14242810 | MS.gene23466:intron | 30.0% |
| AAAACATTTGCATCACACCT+TGG | - | chr7.3:14243025-14243044 | None:intergenic | 35.0% | |
| AAAGGGCAGTGTTAAATTAG+TGG | - | chr7.3:14243134-14243153 | None:intergenic | 35.0% | |
| AACTACAATAAGTCGTTGAC+TGG | - | chr7.3:14242660-14242679 | None:intergenic | 35.0% | |
| AAGTCTATGTTAAAATGGGG+AGG | + | chr7.3:14242181-14242200 | MS.gene23466:intron | 35.0% | |
| AATAGCAAAGAAAGAGAGAG+AGG | - | chr7.3:14242991-14243010 | None:intergenic | 35.0% | |
| ATTGTAGGAAATTGGATCGT+GGG | - | chr7.3:14243202-14243221 | None:intergenic | 35.0% | |
| ATTTGTCATCAGGTGAAAAC+CGG | - | chr7.3:14243263-14243282 | None:intergenic | 35.0% | |
| CTAAGGTAAACCTTGAATCA+AGG | + | chr7.3:14241732-14241751 | MS.gene23466:intron | 35.0% | |
| GGATTTGCAATAGGAAACAA+GGG | - | chr7.3:14242555-14242574 | None:intergenic | 35.0% | |
| GTTAAACTGAAGAAGCGATT+TGG | + | chr7.3:14243297-14243316 | MS.gene23466:CDS | 35.0% | |
| TCGGAATTTGGATTTGCAAT+AGG | - | chr7.3:14242564-14242583 | None:intergenic | 35.0% | |
| TGATTCAAGGTTTACCTTAG+TGG | - | chr7.3:14241732-14241751 | None:intergenic | 35.0% | |
| TGGATTTGCAATAGGAAACA+AGG | - | chr7.3:14242556-14242575 | None:intergenic | 35.0% | |
| TGTAACCAAAATCACTGTGT+GGG | - | chr7.3:14242786-14242805 | None:intergenic | 35.0% | |
| TTGGATTTGCAATCGGAATT+TGG | - | chr7.3:14242576-14242595 | None:intergenic | 35.0% | |
| TTGTAACCAAAATCACTGTG+TGG | - | chr7.3:14242787-14242806 | None:intergenic | 35.0% | |
| TTTGCAATTAGAATTGGGAG+TGG | + | chr7.3:14242318-14242337 | MS.gene23466:intron | 35.0% | |
| ! | CTGATGCATTTTCCTTATTG+GGG | + | chr7.3:14242882-14242901 | MS.gene23466:intron | 35.0% |
| ! | TGACACACATCCAATTTTTG+AGG | + | chr7.3:14242069-14242088 | MS.gene23466:intron | 35.0% |
| ! | TTGGATTTGCGATTGGTTAT+AGG | + | chr7.3:14242132-14242151 | MS.gene23466:intron | 35.0% |
| !! | CGTGGTTTTGCAATTAGAAT+TGG | + | chr7.3:14242312-14242331 | MS.gene23466:intron | 35.0% |
| !! | GGTGATTTTAGCAAAATACC+TGG | - | chr7.3:14241897-14241916 | None:intergenic | 35.0% |
| !! | TGCTCTGAAAATTTGTCATC+AGG | - | chr7.3:14243273-14243292 | None:intergenic | 35.0% |
| AAAATCACTGTGTGGGGAAA+AGG | - | chr7.3:14242779-14242798 | None:intergenic | 40.0% | |
| AATAGGAAACAAGGGATGGT+AGG | - | chr7.3:14242547-14242566 | None:intergenic | 40.0% | |
| AGACAGAGAGACTTCAAACA+TGG | + | chr7.3:14242284-14242303 | MS.gene23466:intron | 40.0% | |
| AGGCGAAGTAAGCAAATTGA+TGG | - | chr7.3:14242614-14242633 | None:intergenic | 40.0% | |
| ATGGGTCGTGATAGAAACTT+TGG | - | chr7.3:14242595-14242614 | None:intergenic | 40.0% | |
| CATTGTAGGAAATTGGATCG+TGG | - | chr7.3:14243203-14243222 | None:intergenic | 40.0% | |
| GCAATGAGAATTAAGAGTGG+CGG | - | chr7.3:14242436-14242455 | None:intergenic | 40.0% | |
| GCACATTTGAGAATTGAGAG+TGG | - | chr7.3:14242414-14242433 | None:intergenic | 40.0% | |
| GGCGAAGTAAGCAAATTGAT+GGG | - | chr7.3:14242613-14242632 | None:intergenic | 40.0% | |
| GTAACCAAAATCACTGTGTG+GGG | - | chr7.3:14242785-14242804 | None:intergenic | 40.0% | |
| GTTGGATTTGGATTTGCGAT+TGG | + | chr7.3:14242125-14242144 | MS.gene23466:intron | 40.0% | |
| TCAGCTAGAAACGTTGGATT+TGG | + | chr7.3:14242113-14242132 | MS.gene23466:intron | 40.0% | |
| TGTTAAAATGGGGAGGCAAT+TGG | + | chr7.3:14242188-14242207 | MS.gene23466:intron | 40.0% | |
| TTGCAATAGGAAACAAGGGA+TGG | - | chr7.3:14242551-14242570 | None:intergenic | 40.0% | |
| TTTGTCATCAGGTGAAAACC+GGG | - | chr7.3:14243262-14243281 | None:intergenic | 40.0% | |
| ! | CATTAACGATAACGGTGACA+AGG | + | chr7.3:14241700-14241719 | MS.gene23466:CDS | 40.0% |
| ! | TCAATTTGCTTACTTCGCCT+TGG | + | chr7.3:14242614-14242633 | MS.gene23466:intron | 40.0% |
| ! | TTTGCGATTGGTTATAGGAG+AGG | + | chr7.3:14242137-14242156 | MS.gene23466:intron | 40.0% |
| ! | TTTTCCCCACACAGTGATTT+TGG | + | chr7.3:14242778-14242797 | MS.gene23466:intron | 40.0% |
| !! | GCACTCTTTTGATTCTGCAT+TGG | + | chr7.3:14243097-14243116 | MS.gene23466:intron | 40.0% |
| GAGAGACTTCAAACATGGAG+AGG | + | chr7.3:14242289-14242308 | MS.gene23466:intron | 45.0% | |
| GATAGGTCAGCTAGAAACGT+TGG | + | chr7.3:14242107-14242126 | MS.gene23466:intron | 45.0% | |
| GATTGAGTAGCTAACCCAAG+CGG | - | chr7.3:14241867-14241886 | None:intergenic | 45.0% | |
| GCAATTAGAATTGGGAGTGG+CGG | + | chr7.3:14242321-14242340 | MS.gene23466:intron | 45.0% | |
| TCAACACACGTCAATCTCCA+AGG | - | chr7.3:14242634-14242653 | None:intergenic | 45.0% | |
| TGACAAGGTCTACACCACTA+AGG | + | chr7.3:14241715-14241734 | MS.gene23466:CDS | 45.0% | |
| TGGAGATTGACGTGTGTTGA+TGG | + | chr7.3:14242634-14242653 | MS.gene23466:intron | 45.0% | |
| ! | TGGCATGGACACATGAGTTT+TGG | - | chr7.3:14242745-14242764 | None:intergenic | 45.0% |
| !! | AAGGTCTTTGAAGCATGGCA+TGG | - | chr7.3:14242760-14242779 | None:intergenic | 45.0% |
| !! | AATACTTTGGAGCTGGATGC+TGG | - | chr7.3:14243335-14243354 | None:intergenic | 45.0% |
| !! | ATACTTTGGAGCTGGATGCT+GGG | - | chr7.3:14243334-14243353 | None:intergenic | 45.0% |
| !! | GGGAAAAGGTCTTTGAAGCA+TGG | - | chr7.3:14242765-14242784 | None:intergenic | 45.0% |
| ACTTCAAACATGGAGAGGCG+TGG | + | chr7.3:14242294-14242313 | MS.gene23466:intron | 50.0% | |
| GGTCTAGAGAAGCCCCAATA+AGG | - | chr7.3:14242897-14242916 | None:intergenic | 50.0% | |
| TGCAGAAAGAGTCACCGCTT+GGG | + | chr7.3:14241850-14241869 | MS.gene23466:intron | 50.0% | |
| TTGCAGAAAGAGTCACCGCT+TGG | + | chr7.3:14241849-14241868 | MS.gene23466:intron | 50.0% | |
| ! | TGTTTTCGGAAACTGCAGCC+CGG | + | chr7.3:14243241-14243260 | MS.gene23466:intron | 50.0% |
| GCTACTCAATCTGCCCATCC+AGG | + | chr7.3:14241876-14241895 | MS.gene23466:CDS | 55.0% | |
| ! | TTTGGAGCTGGATGCTGGGT+CGG | - | chr7.3:14243330-14243349 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 14241683 | 14243356 | 14241683 | ID=MS.gene23466 |
| chr7.3 | mRNA | 14241683 | 14243356 | 14241683 | ID=MS.gene23466.t1;Parent=MS.gene23466 |
| chr7.3 | exon | 14241683 | 14241736 | 14241683 | ID=MS.gene23466.t1.exon1;Parent=MS.gene23466.t1 |
| chr7.3 | CDS | 14241683 | 14241736 | 14241683 | ID=cds.MS.gene23466.t1;Parent=MS.gene23466.t1 |
| chr7.3 | exon | 14241855 | 14241897 | 14241855 | ID=MS.gene23466.t1.exon2;Parent=MS.gene23466.t1 |
| chr7.3 | CDS | 14241855 | 14241897 | 14241855 | ID=cds.MS.gene23466.t1;Parent=MS.gene23466.t1 |
| chr7.3 | exon | 14243259 | 14243356 | 14243259 | ID=MS.gene23466.t1.exon3;Parent=MS.gene23466.t1 |
| chr7.3 | CDS | 14243259 | 14243356 | 14243259 | ID=cds.MS.gene23466.t1;Parent=MS.gene23466.t1 |
| Gene Sequence |
| Protein sequence |
