AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25797

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25797.t1 MTR_3g080410 96.409 362 13 0 1 362 1 362 0.0 704
MS.gene25797.t1 MTR_5g085580 73.977 342 89 0 20 361 19 360 0.0 528
MS.gene25797.t1 MTR_1g056640 66.361 327 110 0 22 348 34 360 4.05e-158 453
MS.gene25797.t1 MTR_1g077990 49.091 330 164 4 19 347 24 350 1.91e-101 305
MS.gene25797.t1 MTR_4g084480 44.875 361 189 5 3 362 4 355 2.29e-101 305
MS.gene25797.t1 MTR_4g084480 44.875 361 189 5 3 362 11 362 3.74e-101 304
MS.gene25797.t1 MTR_4g084480 44.875 361 189 5 3 362 11 362 3.96e-101 304
MS.gene25797.t1 MTR_4g052970 47.164 335 165 5 22 353 25 350 8.53e-101 303
MS.gene25797.t1 MTR_2g086530 43.750 336 184 3 9 343 12 343 2.99e-95 289
MS.gene25797.t1 MTR_2g086530 45.031 322 172 3 23 343 5 322 3.22e-95 288
MS.gene25797.t1 MTR_4g010200 44.986 349 186 4 4 351 13 356 1.20e-94 288
MS.gene25797.t1 MTR_2g019345 45.614 342 178 6 7 343 1 339 1.72e-93 285
MS.gene25797.t1 MTR_6g088885 41.143 350 204 2 1 349 1 349 1.77e-91 283
MS.gene25797.t1 MTR_7g053500 43.925 321 175 4 27 343 27 346 2.02e-91 281
MS.gene25797.t1 MTR_1g007810 45.141 319 168 4 26 342 16 329 8.94e-90 274
MS.gene25797.t1 MTR_1g031900 41.714 350 199 3 4 349 1 349 1.06e-89 278
MS.gene25797.t1 MTR_4g069940 42.982 342 188 4 4 343 10 346 1.25e-89 277
MS.gene25797.t1 MTR_1g031900 41.714 350 199 3 4 349 1 349 1.40e-89 278
MS.gene25797.t1 MTR_8g012400 40.698 344 199 4 4 343 7 349 1.37e-88 274
MS.gene25797.t1 MTR_3g065460 42.210 353 194 4 1 349 3 349 4.06e-88 274
MS.gene25797.t1 MTR_4g088155 39.595 346 202 3 7 346 4 348 1.69e-87 271
MS.gene25797.t1 MTR_3g116510 42.945 326 183 3 20 343 21 345 1.09e-86 269
MS.gene25797.t1 MTR_4g114850 42.683 328 186 2 19 345 19 345 2.16e-85 263
MS.gene25797.t1 MTR_4g132280 40.449 356 193 4 4 341 8 362 2.29e-85 268
MS.gene25797.t1 MTR_4g114850 43.125 320 180 2 27 345 27 345 2.86e-85 263
MS.gene25797.t1 MTR_4g114850 43.125 320 180 2 27 345 27 345 3.17e-85 263
MS.gene25797.t1 MTR_1g022160 42.812 320 180 3 26 343 24 342 3.98e-85 264
MS.gene25797.t1 MTR_1g022160 42.812 320 180 3 26 343 24 342 1.99e-84 264
MS.gene25797.t1 MTR_1g022160 42.812 320 180 3 26 343 24 342 2.04e-84 264
MS.gene25797.t1 MTR_1g022160 42.812 320 180 3 26 343 24 342 2.53e-84 264
MS.gene25797.t1 MTR_5g078200 41.964 336 186 3 1 333 3 332 5.17e-82 262
MS.gene25797.t1 MTR_3g083580 38.415 328 197 3 23 346 23 349 2.53e-81 256
MS.gene25797.t1 MTR_4g083500 44.118 340 179 8 26 362 44 375 5.28e-81 257
MS.gene25797.t1 MTR_7g026340 43.302 321 173 6 24 342 21 334 5.90e-81 254
MS.gene25797.t1 MTR_4g076570 42.679 321 177 4 23 343 19 332 1.40e-80 250
MS.gene25797.t1 MTR_1g052430 42.901 324 176 5 21 343 27 342 1.44e-78 247
MS.gene25797.t1 MTR_5g078200 43.000 300 167 2 37 333 1 299 1.81e-78 252
MS.gene25797.t1 MTR_8g085720 37.971 345 203 3 4 345 11 347 4.27e-78 248
MS.gene25797.t1 MTR_8g102340 40.303 330 162 3 20 349 22 316 5.52e-78 248
MS.gene25797.t1 MTR_4g076470 42.368 321 171 8 25 345 22 328 1.12e-77 242
MS.gene25797.t1 MTR_4g076255 43.210 324 164 8 25 345 24 330 9.76e-77 239
MS.gene25797.t1 MTR_4g076490 42.042 333 180 7 25 357 96 415 5.51e-76 241
MS.gene25797.t1 MTR_4g076440 41.840 337 181 9 10 345 6 328 2.40e-75 236
MS.gene25797.t1 MTR_4g076430 42.857 322 168 9 25 345 22 328 3.65e-75 236
MS.gene25797.t1 MTR_7g053500 44.610 269 144 4 79 343 2 269 1.48e-74 236
MS.gene25797.t1 MTR_4g076500 40.446 314 178 4 22 335 74 378 1.07e-73 234
MS.gene25797.t1 MTR_2g034470 39.755 327 184 8 25 349 29 344 5.08e-71 226
MS.gene25797.t1 MTR_8g012400 42.007 269 151 4 79 343 2 269 1.46e-69 223
MS.gene25797.t1 MTR_2g034480 40.964 332 179 10 25 352 26 344 6.33e-69 221
MS.gene25797.t1 MTR_2g034470 39.185 319 181 8 33 349 2 309 1.15e-67 216
MS.gene25797.t1 MTR_2g034440 39.117 317 182 7 25 339 21 328 1.13e-66 214
MS.gene25797.t1 MTR_7g081350 37.135 342 205 7 1 341 1 333 1.16e-66 214
MS.gene25797.t1 MTR_7g081370 37.101 345 189 9 1 341 1 321 1.19e-61 201
MS.gene25797.t1 MTR_3g116510 45.283 212 115 1 132 343 1 211 1.19e-59 195
MS.gene25797.t1 MTR_2g027560 37.651 332 193 9 20 343 22 347 1.10e-55 189
MS.gene25797.t1 MTR_4g112025 35.897 351 207 12 2 343 9 350 3.04e-53 184
MS.gene25797.t1 MTR_8g092070 34.139 331 204 10 26 349 34 357 1.78e-51 180
MS.gene25797.t1 MTR_1g010120 36.697 327 189 11 26 343 26 343 4.80e-51 177
MS.gene25797.t1 MTR_4g124440 34.637 358 209 13 4 352 11 352 1.34e-50 176
MS.gene25797.t1 MTR_7g085390 34.097 349 205 13 1 336 1 337 4.69e-50 174
MS.gene25797.t1 MTR_6g032820 35.043 351 196 15 3 338 8 341 1.38e-48 169
MS.gene25797.t1 MTR_1g053320 34.650 329 196 11 26 343 30 350 9.68e-47 166
MS.gene25797.t1 MTR_3g095050 32.670 352 216 15 9 349 14 355 2.38e-45 162
MS.gene25797.t1 MTR_3g108600 34.940 332 183 11 19 343 24 329 7.53e-45 160
MS.gene25797.t1 MTR_5g081720 32.131 305 195 8 42 342 53 349 1.36e-43 155
MS.gene25797.t1 MTR_3g031770 34.154 325 200 9 25 343 26 342 1.14e-42 155
MS.gene25797.t1 MTR_4g134280 37.768 233 133 9 114 341 3 228 1.52e-37 139
MS.gene25797.t1 MTR_4g036775 41.346 104 56 2 240 343 77 175 3.99e-17 79.7
MS.gene25797.t1 MTR_4g036775 43.478 69 39 0 24 92 20 88 1.77e-11 63.5
MS.gene25797.t1 MTR_4g036680 41.346 104 56 2 240 343 77 175 8.94e-17 79.0
MS.gene25797.t1 MTR_4g036680 43.478 69 39 0 23 91 19 87 1.48e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25797.t1 AT2G05790 73.054 334 90 0 22 355 21 354 0.0 531
MS.gene25797.t1 AT5G55180 68.421 323 102 0 21 343 22 344 1.44e-158 453
MS.gene25797.t1 AT5G55180 68.421 323 102 0 21 343 22 344 1.63e-158 452
MS.gene25797.t1 AT4G26830 63.905 338 120 2 9 346 9 344 3.07e-148 426
MS.gene25797.t1 AT4G26830 64.923 325 113 1 22 346 25 348 3.87e-148 426
MS.gene25797.t1 AT1G32860 48.160 326 164 4 25 347 28 351 2.00e-103 311
MS.gene25797.t1 AT2G27500 46.386 332 171 3 23 353 26 351 8.24e-100 300
MS.gene25797.t1 AT2G27500 46.386 332 171 3 23 353 26 351 1.73e-99 300
MS.gene25797.t1 AT4G18340 45.245 347 181 4 8 347 10 354 2.81e-98 296
MS.gene25797.t1 AT4G18340 45.376 346 180 4 8 346 10 353 4.58e-98 296
MS.gene25797.t1 AT1G30080 45.115 348 182 4 3 343 7 352 4.66e-98 296
MS.gene25797.t1 AT1G30080 47.352 321 166 3 24 343 34 352 7.71e-98 296
MS.gene25797.t1 AT5G24318 46.106 321 166 4 25 343 29 344 9.16e-97 292
MS.gene25797.t1 AT5G42100 47.447 333 167 6 15 343 18 346 1.26e-96 294
MS.gene25797.t1 AT5G42100 47.447 333 167 6 15 343 18 346 1.43e-96 293
MS.gene25797.t1 AT5G24318 44.838 339 180 4 25 361 29 362 8.23e-96 293
MS.gene25797.t1 AT5G24318 44.838 339 180 4 25 361 29 362 1.63e-95 292
MS.gene25797.t1 AT3G15800 47.561 328 167 4 27 352 44 368 2.08e-95 290
MS.gene25797.t1 AT2G16230 45.031 322 175 2 26 346 25 345 4.10e-92 285
MS.gene25797.t1 AT5G42720 44.638 345 182 4 4 346 10 347 1.15e-91 281
MS.gene25797.t1 AT2G26600 43.114 334 185 3 16 345 21 353 4.27e-90 276
MS.gene25797.t1 AT5G56590 41.104 326 189 3 23 346 23 347 3.68e-87 272
MS.gene25797.t1 AT4G29360 43.149 343 192 2 6 346 15 356 1.14e-86 272
MS.gene25797.t1 AT4G14080 41.390 331 190 3 19 346 35 364 2.38e-86 269
MS.gene25797.t1 AT4G34480 44.828 319 174 2 26 343 26 343 3.47e-86 265
MS.gene25797.t1 AT4G29360 41.449 345 201 1 2 346 3 346 1.18e-85 269
MS.gene25797.t1 AT2G01630 41.284 327 188 2 26 349 23 348 1.22e-84 262
MS.gene25797.t1 AT3G07320 40.841 333 190 5 27 355 26 355 1.42e-84 264
MS.gene25797.t1 AT2G01630 41.284 327 188 2 26 349 23 348 2.52e-84 263
MS.gene25797.t1 AT4G34480 44.828 319 174 2 26 343 26 343 2.71e-84 265
MS.gene25797.t1 AT3G23770 40.469 341 194 4 19 353 30 367 2.93e-84 264
MS.gene25797.t1 AT2G01630 41.284 327 188 2 26 349 23 348 2.54e-83 262
MS.gene25797.t1 AT1G66250 40.896 335 193 3 18 349 24 356 2.76e-83 262
MS.gene25797.t1 AT1G11820 39.812 319 188 3 26 341 44 361 7.56e-81 252
MS.gene25797.t1 AT3G13560 40.244 328 190 4 19 341 17 343 2.68e-80 254
MS.gene25797.t1 AT3G13560 40.244 328 190 4 19 341 17 343 2.68e-80 254
MS.gene25797.t1 AT3G13560 40.244 328 190 4 19 341 17 343 2.68e-80 254
MS.gene25797.t1 AT3G13560 40.244 328 190 4 19 341 17 343 2.68e-80 254
MS.gene25797.t1 AT3G13560 40.244 328 190 4 19 341 17 343 2.68e-80 254
MS.gene25797.t1 AT1G11820 39.812 319 188 3 26 341 44 361 9.92e-80 252
MS.gene25797.t1 AT1G11820 39.812 319 188 3 26 341 44 361 2.26e-79 252
MS.gene25797.t1 AT2G27500 44.604 278 147 3 77 353 2 273 1.53e-78 244
MS.gene25797.t1 AT4G16260 39.420 345 197 7 4 348 5 337 5.45e-76 238
MS.gene25797.t1 AT3G57270 40.923 325 181 7 23 345 24 339 6.72e-73 230
MS.gene25797.t1 AT3G46570 41.714 350 183 12 1 343 7 342 1.54e-71 227
MS.gene25797.t1 AT4G16260 40.190 316 180 6 33 348 2 308 4.66e-71 224
MS.gene25797.t1 AT3G55430 38.988 336 186 12 14 343 19 341 2.93e-70 227
MS.gene25797.t1 AT2G26600 42.913 254 143 2 93 345 7 259 2.85e-69 219
MS.gene25797.t1 AT3G61810 37.690 329 186 5 26 346 55 372 6.90e-69 221
MS.gene25797.t1 AT1G77780 39.650 343 192 9 4 343 2 332 5.82e-66 213
MS.gene25797.t1 AT3G57260 37.719 342 196 8 4 345 14 338 1.83e-65 211
MS.gene25797.t1 AT3G57260 37.719 342 196 8 4 345 30 354 3.78e-65 211
MS.gene25797.t1 AT5G24318 44.934 227 121 1 135 361 1 223 3.46e-64 207
MS.gene25797.t1 AT2G39640 36.047 344 206 10 3 343 6 338 1.09e-60 204
MS.gene25797.t1 AT1G77790 36.443 343 208 7 3 343 7 341 2.52e-59 195
MS.gene25797.t1 AT3G57240 37.188 320 187 8 26 345 35 340 5.19e-59 194
MS.gene25797.t1 AT5G20390 36.657 341 204 9 3 341 11 341 9.60e-59 194
MS.gene25797.t1 AT4G34480 47.186 231 120 2 114 343 3 232 2.85e-58 194
MS.gene25797.t1 AT5G20330 39.144 327 188 8 19 343 27 344 3.09e-58 192
MS.gene25797.t1 AT5G20340 38.066 331 192 9 16 343 33 353 5.29e-56 187
MS.gene25797.t1 AT3G24330 33.520 358 219 12 2 349 16 364 4.24e-54 186
MS.gene25797.t1 AT1G33220 36.310 336 195 7 9 343 17 334 3.55e-53 179
MS.gene25797.t1 AT5G18220 33.898 354 214 11 5 351 13 353 4.17e-51 177
MS.gene25797.t1 AT4G17180 34.402 343 204 11 24 357 23 353 9.13e-51 176
MS.gene25797.t1 AT2G19440 35.088 342 198 14 19 349 16 344 1.13e-50 176
MS.gene25797.t1 AT2G19440 35.088 342 198 14 19 349 16 344 1.13e-50 176
MS.gene25797.t1 AT3G55780 29.375 320 216 7 42 356 51 365 8.74e-50 171
MS.gene25797.t1 AT1G64760 34.706 340 202 14 24 353 23 352 3.26e-49 172
MS.gene25797.t1 AT1G64760 34.706 340 202 14 24 353 23 352 3.26e-49 172
MS.gene25797.t1 AT5G58090 36.420 324 184 13 24 336 20 332 1.08e-48 171
MS.gene25797.t1 AT5G64790 34.181 354 215 11 4 349 8 351 5.69e-48 169
MS.gene25797.t1 AT4G31140 34.006 347 212 13 19 356 20 358 2.34e-47 167
MS.gene25797.t1 AT3G04010 32.493 357 221 12 6 353 15 360 5.44e-46 164
MS.gene25797.t1 AT5G20870 33.618 351 207 14 2 336 7 347 1.51e-45 163
MS.gene25797.t1 AT5G20560 33.134 335 211 9 4 335 12 336 3.15e-44 155
MS.gene25797.t1 AT5G58480 33.131 329 204 9 25 345 25 345 9.94e-43 155

Find 100 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTACTTCAGAGAGGAATTT+TGG 0.105755 3.2:+70576420 MS.gene25797:CDS
GATGGCTTCTATTTGGGTTT+TGG 0.211284 3.2:-70575770 None:intergenic
CGACGGCGATGGCTTCTATT+TGG 0.212947 3.2:-70575777 None:intergenic
CATGGCGGGGATGAGGTATT+TGG 0.229684 3.2:-70575833 None:intergenic
TCCTCTTATCCGTCTTCAAC+CGG 0.271730 3.2:+70575940 MS.gene25797:CDS
TCATAAACTTTCTTCATATC+AGG 0.295925 3.2:-70576453 None:intergenic
GACGGCGATGGCTTCTATTT+GGG 0.306420 3.2:-70575776 None:intergenic
TTCAACCCCGCCACAGTAAA+CGG 0.314456 3.2:-70576480 None:intergenic
AGATTCGCATTCGGTCTTAA+AGG 0.324788 3.2:-70576351 None:intergenic
TTTAGCATTGGTTTAATTAC+GGG 0.335880 3.2:-70575988 None:intergenic
GTTTAGCATTGGTTTAATTA+CGG 0.353752 3.2:-70575989 None:intergenic
GATTCGCATTCGGTCTTAAA+GGG 0.355739 3.2:-70576350 None:intergenic
CTCTCACTTCCACTACTTGC+AGG 0.379057 3.2:+70575226 MS.gene25797:CDS
CGCTTTGTTTCGTGAAAACC+CGG 0.382673 3.2:+70576101 MS.gene25797:CDS
AGTTCTGCTCAGCCGCTTCC+CGG 0.386123 3.2:-70576525 None:intergenic
GTTTACGGTTACTTTGATTC+CGG 0.388759 3.2:-70575662 None:intergenic
TGAAACGTCGTCGTATTTGA+TGG 0.393298 3.2:+70576020 MS.gene25797:CDS
TACCGTGACGTTTCGGCGCC+GGG 0.395980 3.2:+70576507 MS.gene25797:CDS
GACGTTTCGGCGCCGGGAAG+CGG 0.399867 3.2:+70576513 MS.gene25797:CDS
TTGTTCGTTTGGGAGGTTTA+CGG 0.405496 3.2:-70575677 None:intergenic
TATAGAAGAGTTGTTCGTTT+GGG 0.414891 3.2:-70575687 None:intergenic
TTTGATTCCGGATCCGGATA+AGG 0.415353 3.2:-70575650 None:intergenic
GAAGAGTTACCGTGACGTTT+CGG 0.420176 3.2:+70576500 MS.gene25797:CDS
AATTCCATTGGAGTGAACTA+TGG 0.424527 3.2:+70575520 MS.gene25797:CDS
GGTTACTTTGATTCCGGATC+CGG 0.435065 3.2:-70575656 None:intergenic
GATGTCCCGTTTACTGTGGC+GGG 0.446901 3.2:+70576474 MS.gene25797:CDS
GGTAATGTGGATCCGGGTAA+CGG 0.452916 3.2:+70576123 MS.gene25797:CDS
TCAAATACGACGACGTTTCA+CGG 0.456148 3.2:-70576017 None:intergenic
GTTACAGATGGTAATTCCAT+TGG 0.459097 3.2:+70575508 MS.gene25797:intron
ATTACGGGTTCTATGAGATC+GGG 0.459527 3.2:-70575973 None:intergenic
TAGTGGAAGTGAGAGGTATG+AGG 0.464913 3.2:-70575218 None:intergenic
CCCGGTTGAAGACGGATAAG+AGG 0.467032 3.2:-70575941 None:intergenic
GTAATGTGGATCCGGGTAAC+GGG 0.469921 3.2:+70576124 MS.gene25797:CDS
CTAAAATCCTTATCCGGATC+CGG 0.470230 3.2:+70575643 MS.gene25797:CDS
AAACCCGGGTAATGTGGATC+CGG 0.471625 3.2:+70576116 MS.gene25797:CDS
GTGAGAGGTATGAGGAAATG+AGG 0.472680 3.2:-70575210 None:intergenic
TCAACCCCGCCACAGTAAAC+GGG 0.482442 3.2:-70576479 None:intergenic
AGAGTGGTTGTGAGTGAAAC+GGG 0.485100 3.2:+70576225 MS.gene25797:CDS
GTATAGAAGAGTTGTTCGTT+TGG 0.487771 3.2:-70575688 None:intergenic
TTGTACGCAGCAGCGTTCTG+TGG 0.488748 3.2:-70576288 None:intergenic
AATTACGGGTTCTATGAGAT+CGG 0.488872 3.2:-70575974 None:intergenic
TTACCGTGACGTTTCGGCGC+CGG 0.490715 3.2:+70576506 MS.gene25797:CDS
TTTAATGAAAATGGGAAAGT+GGG 0.491545 3.2:+70576396 MS.gene25797:CDS
TGTACGCAGCAGCGTTCTGT+GGG 0.494413 3.2:-70576287 None:intergenic
CCAAAATTCCTCTCTGAAGT+AGG 0.497611 3.2:-70576420 None:intergenic
TAAAATCACAAGGAATCGAC+CGG 0.501310 3.2:+70575590 MS.gene25797:CDS
GCCGTTCTAAAATCCTTATC+CGG 0.508050 3.2:+70575637 MS.gene25797:CDS
TAGAGTGGTTGTGAGTGAAA+CGG 0.511502 3.2:+70576224 MS.gene25797:CDS
AGAAGAGTTGTTCGTTTGGG+AGG 0.520427 3.2:-70575684 None:intergenic
CAAATAGAAGCCATCGCCGT+CGG 0.523034 3.2:+70575778 MS.gene25797:CDS
CCAATCGCTTTATCTGCTCT+CGG 0.531316 3.2:+70575916 MS.gene25797:CDS
GACTTCATTACCGACGGCGA+TGG 0.533265 3.2:-70575788 None:intergenic
CTACTTTGAATGCTGAGGGT+AGG 0.540473 3.2:-70575558 None:intergenic
TTACCCGGATCCACATTACC+CGG 0.542092 3.2:-70576120 None:intergenic
AACCCGGGTAATGTGGATCC+GGG 0.543293 3.2:+70576117 MS.gene25797:CDS
GAGGTATGAGGAAATGAGGA+TGG 0.545090 3.2:-70575206 None:intergenic
CTTAACTACTTTGAATGCTG+AGG 0.551196 3.2:-70575563 None:intergenic
AACTTTCCTACGCCCTCACA+TGG 0.552284 3.2:+70575719 MS.gene25797:CDS
TGATGTCCCGTTTACTGTGG+CGG 0.556256 3.2:+70576473 MS.gene25797:CDS
AACGTCAGCGTTTGACTCGT+AGG 0.557317 3.2:-70576064 None:intergenic
TCGTGAAAACCCGGGTAATG+TGG 0.558115 3.2:+70576110 MS.gene25797:CDS
TTGGAGCCATGTGAGGGCGT+AGG 0.561253 3.2:-70575725 None:intergenic
TTGAATGACCCGGTTGAAGA+CGG 0.562405 3.2:-70575949 None:intergenic
TAAACGGTCAGATTCGCATT+CGG 0.565144 3.2:-70576360 None:intergenic
CCGAGAGCAGATAAAGCGAT+TGG 0.569316 3.2:-70575916 None:intergenic
CCTCTTATCCGTCTTCAACC+GGG 0.572031 3.2:+70575941 MS.gene25797:CDS
TCACGGAGGAAGTTTAGCAT+TGG 0.572211 3.2:-70576000 None:intergenic
TTAACTACTTTGAATGCTGA+GGG 0.578124 3.2:-70575562 None:intergenic
TTCCCGGCGCCGAAACGTCA+CGG 0.582794 3.2:-70576509 None:intergenic
GCGACCATAGTTCACTCCAA+TGG 0.590342 3.2:-70575524 None:intergenic
GAAGCAATACCTGCAAGTAG+TGG 0.596051 3.2:-70575235 None:intergenic
CGTCGGTAATGAAGTCTTCG+TGG 0.598441 3.2:+70575795 MS.gene25797:CDS
GGCGGGGATGAGGTATTTGG+TGG 0.601341 3.2:-70575830 None:intergenic
CACGAAGACTTCATTACCGA+CGG 0.613529 3.2:-70575794 None:intergenic
GAAACGGGTTGGCCTTCGAA+AGG 0.613978 3.2:+70576240 MS.gene25797:CDS
TCAATACAACGACGTTAGAG+TGG 0.620974 3.2:+70576209 MS.gene25797:CDS
AGAAGATTCTGAACAACGGT+GGG 0.622512 3.2:+70576325 MS.gene25797:CDS
CGGTGTCGTAGAGTTTGACC+CGG 0.627592 3.2:-70575609 None:intergenic
ATTCGCATTCGGTCTTAAAG+GGG 0.628701 3.2:-70576349 None:intergenic
TTATGATGTCCCGTTTACTG+TGG 0.629738 3.2:+70576470 MS.gene25797:CDS
GAGATCGGGTTTGAATGACC+CGG 0.632773 3.2:-70575959 None:intergenic
CAAACTCTACGACACCGACC+CGG 0.633891 3.2:+70575615 MS.gene25797:CDS
TCATCACTATCACCTTTCGA+AGG 0.639827 3.2:-70576252 None:intergenic
GAAAGGTGATAGTGATGAGG+TGG 0.640804 3.2:+70576257 MS.gene25797:CDS
AAGAATGAGTAGACGATGCA+TGG 0.642954 3.2:-70575182 None:intergenic
TACCCGGATCCACATTACCC+GGG 0.647522 3.2:-70576119 None:intergenic
TTCGAAAGGTGATAGTGATG+AGG 0.659057 3.2:+70576254 MS.gene25797:CDS
CAGAACGCTGCTGCGTACAA+CGG 0.661248 3.2:+70576291 MS.gene25797:CDS
CTGCAAGTAGTGGAAGTGAG+AGG 0.663993 3.2:-70575225 None:intergenic
AAGAAGATTCTGAACAACGG+TGG 0.664425 3.2:+70576324 MS.gene25797:CDS
AAAATCACAAGGAATCGACC+GGG 0.666777 3.2:+70575591 MS.gene25797:CDS
GTTAAGAAGATTCTGAACAA+CGG 0.668546 3.2:+70576321 MS.gene25797:CDS
ATGTCCCGTTTACTGTGGCG+GGG 0.669480 3.2:+70576475 MS.gene25797:CDS
GTTAAGCTTCTAAAATCACA+AGG 0.686975 3.2:+70575580 MS.gene25797:CDS
TGGTTGTGAGTGAAACGGGT+TGG 0.699770 3.2:+70576229 MS.gene25797:CDS
GCTTTGTTTCGTGAAAACCC+GGG 0.717428 3.2:+70576102 MS.gene25797:CDS
GTACGCAGCAGCGTTCTGTG+GGG 0.722190 3.2:-70576286 None:intergenic
AAAGGTGATAGTGATGAGGT+GGG 0.725483 3.2:+70576258 MS.gene25797:CDS
AAGTGGGACCTACTTCAGAG+AGG 0.745668 3.2:+70576412 MS.gene25797:CDS
AATACGACGACGTTTCACGG+AGG 0.753555 3.2:-70576014 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATGTAAACAAAAAAGA+TGG - chr3.2:70575276-70575295 None:intergenic 15.0%
!!! AAAAAGCGCAAATAAATAAA+CGG - chr3.2:70576379-70576398 None:intergenic 20.0%
!!! TTATTAATTGTTGTTACAGA+TGG + chr3.2:70575496-70575515 MS.gene25797:intron 20.0%
! TAATATAAACATGTGTGTGT+AGG + chr3.2:70575329-70575348 MS.gene25797:intron 25.0%
! TCATAAACTTTCTTCATATC+AGG - chr3.2:70576456-70576475 None:intergenic 25.0%
! TTTAATGAAAATGGGAAAGT+GGG + chr3.2:70576396-70576415 MS.gene25797:CDS 25.0%
!! TTTTAATGAAAATGGGAAAG+TGG + chr3.2:70576395-70576414 MS.gene25797:CDS 25.0%
!!! GTTTAGCATTGGTTTAATTA+CGG - chr3.2:70575992-70576011 None:intergenic 25.0%
!!! TTGCGCTTTTTAATGAAAAT+GGG + chr3.2:70576388-70576407 MS.gene25797:CDS 25.0%
!!! TTTAGCATTGGTTTAATTAC+GGG - chr3.2:70575991-70576010 None:intergenic 25.0%
!!! TTTGCGCTTTTTAATGAAAA+TGG + chr3.2:70576387-70576406 MS.gene25797:CDS 25.0%
GTTAAGAAGATTCTGAACAA+CGG + chr3.2:70576321-70576340 MS.gene25797:CDS 30.0%
TATAGAAGAGTTGTTCGTTT+GGG - chr3.2:70575690-70575709 None:intergenic 30.0%
TGTATGTGAAAGTGACTTAT+CGG - chr3.2:70575365-70575384 None:intergenic 30.0%
TTAACTACTTTGAATGCTGA+GGG - chr3.2:70575565-70575584 None:intergenic 30.0%
! TTTGTGAATGTTTTTCATGG+CGG - chr3.2:70575851-70575870 None:intergenic 30.0%
!! AAGAGATTTTGATGGAATTG+TGG - chr3.2:70575894-70575913 None:intergenic 30.0%
!! AGCTTTGTGAATGTTTTTCA+TGG - chr3.2:70575854-70575873 None:intergenic 30.0%
!! GTTAAGCTTCTAAAATCACA+AGG + chr3.2:70575580-70575599 MS.gene25797:CDS 30.0%
!!! TAATATTTTAACCCGTTACC+CGG - chr3.2:70576138-70576157 None:intergenic 30.0%
AATTACGGGTTCTATGAGAT+CGG - chr3.2:70575977-70575996 None:intergenic 35.0%
AATTCCATTGGAGTGAACTA+TGG + chr3.2:70575520-70575539 MS.gene25797:CDS 35.0%
CAAAAAAGATGGTGAAGAAG+TGG - chr3.2:70575265-70575284 None:intergenic 35.0%
CTTAACTACTTTGAATGCTG+AGG - chr3.2:70575566-70575585 None:intergenic 35.0%
GTATAGAAGAGTTGTTCGTT+TGG - chr3.2:70575691-70575710 None:intergenic 35.0%
GTTACAGATGGTAATTCCAT+TGG + chr3.2:70575508-70575527 MS.gene25797:intron 35.0%
TAAAATCACAAGGAATCGAC+CGG + chr3.2:70575590-70575609 MS.gene25797:CDS 35.0%
! CTACTTCAGAGAGGAATTTT+GGG + chr3.2:70576421-70576440 MS.gene25797:CDS 35.0%
! GATTGGTGAAGAGATTTTGA+TGG - chr3.2:70575902-70575921 None:intergenic 35.0%
! GTTTACGGTTACTTTGATTC+CGG - chr3.2:70575665-70575684 None:intergenic 35.0%
! TTGTGAATGTTTTTCATGGC+GGG - chr3.2:70575850-70575869 None:intergenic 35.0%
!! AGATTTTGATGGAATTGTGG+AGG - chr3.2:70575891-70575910 None:intergenic 35.0%
!! ATGGCTTCTATTTGGGTTTT+GGG - chr3.2:70575772-70575791 None:intergenic 35.0%
!! TCCGGATAAGGATTTTAGAA+CGG - chr3.2:70575641-70575660 None:intergenic 35.0%
AAAATCACAAGGAATCGACC+GGG + chr3.2:70575591-70575610 MS.gene25797:CDS 40.0%
AAGAAGATTCTGAACAACGG+TGG + chr3.2:70576324-70576343 MS.gene25797:CDS 40.0%
AAGATGGTGAAGAAGTGGAA+TGG - chr3.2:70575260-70575279 None:intergenic 40.0%
AGAAGATTCTGAACAACGGT+GGG + chr3.2:70576325-70576344 MS.gene25797:CDS 40.0%
AGATTCGCATTCGGTCTTAA+AGG - chr3.2:70576354-70576373 None:intergenic 40.0%
ATTACGGGTTCTATGAGATC+GGG - chr3.2:70575976-70575995 None:intergenic 40.0%
ATTCGCATTCGGTCTTAAAG+GGG - chr3.2:70576352-70576371 None:intergenic 40.0%
CTAAAATCCTTATCCGGATC+CGG + chr3.2:70575643-70575662 MS.gene25797:CDS 40.0%
GATTCGCATTCGGTCTTAAA+GGG - chr3.2:70576353-70576372 None:intergenic 40.0%
TAAACGGTCAGATTCGCATT+CGG - chr3.2:70576363-70576382 None:intergenic 40.0%
TAGAGTGGTTGTGAGTGAAA+CGG + chr3.2:70576224-70576243 MS.gene25797:CDS 40.0%
TCAAATACGACGACGTTTCA+CGG - chr3.2:70576020-70576039 None:intergenic 40.0%
TCAATACAACGACGTTAGAG+TGG + chr3.2:70576209-70576228 MS.gene25797:CDS 40.0%
TCATCACTATCACCTTTCGA+AGG - chr3.2:70576255-70576274 None:intergenic 40.0%
TGAAACGTCGTCGTATTTGA+TGG + chr3.2:70576020-70576039 MS.gene25797:CDS 40.0%
TTATGATGTCCCGTTTACTG+TGG + chr3.2:70576470-70576489 MS.gene25797:CDS 40.0%
TTGTTCGTTTGGGAGGTTTA+CGG - chr3.2:70575680-70575699 None:intergenic 40.0%
! AAAGGTGATAGTGATGAGGT+GGG + chr3.2:70576258-70576277 MS.gene25797:CDS 40.0%
! CCTACTTCAGAGAGGAATTT+TGG + chr3.2:70576420-70576439 MS.gene25797:CDS 40.0%
! TGTGAATGTTTTTCATGGCG+GGG - chr3.2:70575849-70575868 None:intergenic 40.0%
! TTCGAAAGGTGATAGTGATG+AGG + chr3.2:70576254-70576273 MS.gene25797:CDS 40.0%
! TTGACTCGTAGGCGAAAAAT+GGG - chr3.2:70576056-70576075 None:intergenic 40.0%
! TTTGACTCGTAGGCGAAAAA+TGG - chr3.2:70576057-70576076 None:intergenic 40.0%
!! CCAAAATTCCTCTCTGAAGT+AGG - chr3.2:70576423-70576442 None:intergenic 40.0%
!! GATGGCTTCTATTTGGGTTT+TGG - chr3.2:70575773-70575792 None:intergenic 40.0%
!! GCCGTTCTAAAATCCTTATC+CGG + chr3.2:70575637-70575656 MS.gene25797:CDS 40.0%
!! TGGCTTCTATTTGGGTTTTG+GGG - chr3.2:70575771-70575790 None:intergenic 40.0%
!!! ATAAGGATTTTAGAACGGCC+GGG - chr3.2:70575636-70575655 None:intergenic 40.0%
!!! GATAAGGATTTTAGAACGGC+CGG - chr3.2:70575637-70575656 None:intergenic 40.0%
!!! GGTAAACGACGACGTTTTTT+TGG - chr3.2:70575747-70575766 None:intergenic 40.0%
!!! GTTTTTTTGGAGCCATGTGA+GGG - chr3.2:70575734-70575753 None:intergenic 40.0%
AGAAGAGTTGTTCGTTTGGG+AGG - chr3.2:70575687-70575706 None:intergenic 45.0%
AGAGTGGTTGTGAGTGAAAC+GGG + chr3.2:70576225-70576244 MS.gene25797:CDS 45.0%
CACGAAGACTTCATTACCGA+CGG - chr3.2:70575797-70575816 None:intergenic 45.0%
CCAATCGCTTTATCTGCTCT+CGG + chr3.2:70575916-70575935 MS.gene25797:CDS 45.0%
CTACTTTGAATGCTGAGGGT+AGG - chr3.2:70575561-70575580 None:intergenic 45.0%
GAAGAGTTACCGTGACGTTT+CGG + chr3.2:70576500-70576519 MS.gene25797:CDS 45.0%
GAAGCAATACCTGCAAGTAG+TGG - chr3.2:70575238-70575257 None:intergenic 45.0%
GAGGTATGAGGAAATGAGGA+TGG - chr3.2:70575209-70575228 None:intergenic 45.0%
TCACGGAGGAAGTTTAGCAT+TGG - chr3.2:70576003-70576022 None:intergenic 45.0%
TCCTCTTATCCGTCTTCAAC+CGG + chr3.2:70575940-70575959 MS.gene25797:CDS 45.0%
TTGAATGACCCGGTTGAAGA+CGG - chr3.2:70575952-70575971 None:intergenic 45.0%
TTTGATTCCGGATCCGGATA+AGG - chr3.2:70575653-70575672 None:intergenic 45.0%
! GAAAGGTGATAGTGATGAGG+TGG + chr3.2:70576257-70576276 MS.gene25797:CDS 45.0%
! GGTTACTTTGATTCCGGATC+CGG - chr3.2:70575659-70575678 None:intergenic 45.0%
! GTGAGAGGTATGAGGAAATG+AGG - chr3.2:70575213-70575232 None:intergenic 45.0%
! TAGTGGAAGTGAGAGGTATG+AGG - chr3.2:70575221-70575240 None:intergenic 45.0%
!! CGCTTTGTTTCGTGAAAACC+CGG + chr3.2:70576101-70576120 MS.gene25797:CDS 45.0%
!! GCTTTGTTTCGTGAAAACCC+GGG + chr3.2:70576102-70576121 MS.gene25797:CDS 45.0%
!!! CGTTTTTTTGGAGCCATGTG+AGG - chr3.2:70575735-70575754 None:intergenic 45.0%
!!! CTTCTATTTGGGTTTTGGGG+TGG - chr3.2:70575768-70575787 None:intergenic 45.0%
!!! GAGGGCGTAGGAAAGTTTTT+TGG - chr3.2:70575716-70575735 None:intergenic 45.0%
AAACCCGGGTAATGTGGATC+CGG + chr3.2:70576116-70576135 MS.gene25797:CDS 50.0%
AACTTTCCTACGCCCTCACA+TGG + chr3.2:70575719-70575738 MS.gene25797:CDS 50.0%
AAGTGGGACCTACTTCAGAG+AGG + chr3.2:70576412-70576431 MS.gene25797:CDS 50.0%
AATACGACGACGTTTCACGG+AGG - chr3.2:70576017-70576036 None:intergenic 50.0%
CAAATAGAAGCCATCGCCGT+CGG + chr3.2:70575778-70575797 MS.gene25797:CDS 50.0%
CCGAGAGCAGATAAAGCGAT+TGG - chr3.2:70575919-70575938 None:intergenic 50.0%
CCTCTTATCCGTCTTCAACC+GGG + chr3.2:70575941-70575960 MS.gene25797:CDS 50.0%
CGTCGGTAATGAAGTCTTCG+TGG + chr3.2:70575795-70575814 MS.gene25797:CDS 50.0%
CTCTCACTTCCACTACTTGC+AGG + chr3.2:70575226-70575245 MS.gene25797:CDS 50.0%
GACGGCGATGGCTTCTATTT+GGG - chr3.2:70575779-70575798 None:intergenic 50.0%
GAGATCGGGTTTGAATGACC+CGG - chr3.2:70575962-70575981 None:intergenic 50.0%
GCGACCATAGTTCACTCCAA+TGG - chr3.2:70575527-70575546 None:intergenic 50.0%
GGTAATGTGGATCCGGGTAA+CGG + chr3.2:70576123-70576142 MS.gene25797:CDS 50.0%
GTAATGTGGATCCGGGTAAC+GGG + chr3.2:70576124-70576143 MS.gene25797:CDS 50.0%
TCGTGAAAACCCGGGTAATG+TGG + chr3.2:70576110-70576129 MS.gene25797:CDS 50.0%
TGATGTCCCGTTTACTGTGG+CGG + chr3.2:70576473-70576492 MS.gene25797:CDS 50.0%
TGGTTGTGAGTGAAACGGGT+TGG + chr3.2:70576229-70576248 MS.gene25797:CDS 50.0%
TTACCCGGATCCACATTACC+CGG - chr3.2:70576123-70576142 None:intergenic 50.0%
TTCAACCCCGCCACAGTAAA+CGG - chr3.2:70576483-70576502 None:intergenic 50.0%
! AACGTCAGCGTTTGACTCGT+AGG - chr3.2:70576067-70576086 None:intergenic 50.0%
! CTGCAAGTAGTGGAAGTGAG+AGG - chr3.2:70575228-70575247 None:intergenic 50.0%
!! TGTTTTTCATGGCGGGGATG+AGG - chr3.2:70575843-70575862 None:intergenic 50.0%
AACCCGGGTAATGTGGATCC+GGG + chr3.2:70576117-70576136 MS.gene25797:CDS 55.0%
ATGTCCCGTTTACTGTGGCG+GGG + chr3.2:70576475-70576494 MS.gene25797:CDS 55.0%
CAAACTCTACGACACCGACC+CGG + chr3.2:70575615-70575634 MS.gene25797:CDS 55.0%
CAGAACGCTGCTGCGTACAA+CGG + chr3.2:70576291-70576310 MS.gene25797:CDS 55.0%
CCCGGTTGAAGACGGATAAG+AGG - chr3.2:70575944-70575963 None:intergenic 55.0%
CGACGGCGATGGCTTCTATT+TGG - chr3.2:70575780-70575799 None:intergenic 55.0%
GACTTCATTACCGACGGCGA+TGG - chr3.2:70575791-70575810 None:intergenic 55.0%
GATGTCCCGTTTACTGTGGC+GGG + chr3.2:70576474-70576493 MS.gene25797:CDS 55.0%
TACCCGGATCCACATTACCC+GGG - chr3.2:70576122-70576141 None:intergenic 55.0%
TCAACCCCGCCACAGTAAAC+GGG - chr3.2:70576482-70576501 None:intergenic 55.0%
TGTACGCAGCAGCGTTCTGT+GGG - chr3.2:70576290-70576309 None:intergenic 55.0%
TTGTACGCAGCAGCGTTCTG+TGG - chr3.2:70576291-70576310 None:intergenic 55.0%
! CATGGCGGGGATGAGGTATT+TGG - chr3.2:70575836-70575855 None:intergenic 55.0%
!! CGGTGTCGTAGAGTTTGACC+CGG - chr3.2:70575612-70575631 None:intergenic 55.0%
!! GAAACGGGTTGGCCTTCGAA+AGG + chr3.2:70576240-70576259 MS.gene25797:CDS 55.0%
!!! GGATTTTAGAACGGCCGGGT+CGG - chr3.2:70575632-70575651 None:intergenic 55.0%
GTACGCAGCAGCGTTCTGTG+GGG - chr3.2:70576289-70576308 None:intergenic 60.0%
TTACCGTGACGTTTCGGCGC+CGG + chr3.2:70576506-70576525 MS.gene25797:CDS 60.0%
! GGCGGGGATGAGGTATTTGG+TGG - chr3.2:70575833-70575852 None:intergenic 60.0%
! TTGGAGCCATGTGAGGGCGT+AGG - chr3.2:70575728-70575747 None:intergenic 60.0%
TACCGTGACGTTTCGGCGCC+GGG + chr3.2:70576507-70576526 MS.gene25797:CDS 65.0%
! TTCCCGGCGCCGAAACGTCA+CGG - chr3.2:70576512-70576531 None:intergenic 65.0%
! GACGTTTCGGCGCCGGGAAG+CGG + chr3.2:70576513-70576532 MS.gene25797:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 70575184 70576539 70575184 ID=MS.gene25797
chr3.2 mRNA 70575184 70576539 70575184 ID=MS.gene25797.t1;Parent=MS.gene25797
chr3.2 exon 70575184 70575247 70575184 ID=MS.gene25797.t1.exon1;Parent=MS.gene25797.t1
chr3.2 CDS 70575184 70575247 70575184 ID=cds.MS.gene25797.t1;Parent=MS.gene25797.t1
chr3.2 exon 70575515 70576539 70575515 ID=MS.gene25797.t1.exon2;Parent=MS.gene25797.t1
chr3.2 CDS 70575515 70576539 70575515 ID=cds.MS.gene25797.t1;Parent=MS.gene25797.t1
Gene Sequence

>MS.gene25797

ATGCATCGTCTACTCATTCTTTCCATCCTCATTTCCTCATACCTCTCACTTCCACTACTTGCAGGTATTGCTTCCATTCCACTTCTTCACCATCTTTTTTGTTTACATTATTGCATCTACTAACATTTCTGATTGAATGCGTGTTTAATATAAACATGTGTGTGTAGGTGTCTTAACACCGATAAGTCACTTTCACATACACACGTGTACATACACTGTATGTGATTACATTTAATTATTATGTTTATATGATAGATAGATTATTGTTACTTGTTTTCATGCATGTATAATGCTAATATTGTTTTCATTTCTTTATTAATTGTTGTTACAGATGGTAATTCCATTGGAGTGAACTATGGTCGCATAGCAAACAACCTACCCTCAGCATTCAAAGTAGTTAAGCTTCTAAAATCACAAGGAATCGACCGGGTCAAACTCTACGACACCGACCCGGCCGTTCTAAAATCCTTATCCGGATCCGGAATCAAAGTAACCGTAAACCTCCCAAACGAACAACTCTTCTATACAGCCAAAAAACTTTCCTACGCCCTCACATGGCTCCAAAAAAACGTCGTCGTTTACCACCCCAAAACCCAAATAGAAGCCATCGCCGTCGGTAATGAAGTCTTCGTGGACACACACAACACCACCAAATACCTCATCCCCGCCATGAAAAACATTCACAAAGCTTTAATCAAATTCAACCTCCACAATTCCATCAAAATCTCTTCACCAATCGCTTTATCTGCTCTCGGATCCTCTTATCCGTCTTCAACCGGGTCATTCAAACCCGATCTCATAGAACCCGTAATTAAACCAATGCTAAACTTCCTCCGTGAAACGTCGTCGTATTTGATGGTCAACGTTTACCCATTTTTCGCCTACGAGTCAAACGCTGACGTTATTTCTTTAAACTACGCTTTGTTTCGTGAAAACCCGGGTAATGTGGATCCGGGTAACGGGTTAAAATATTACAACATCTTCGACGCACAAATCGACGCTGTTTTTGCAGCGCTTAACAAGCTTCAATACAACGACGTTAGAGTGGTTGTGAGTGAAACGGGTTGGCCTTCGAAAGGTGATAGTGATGAGGTGGGTGCGTCCCCACAGAACGCTGCTGCGTACAACGGTAATCTTGTTAAGAAGATTCTGAACAACGGTGGGACCCCTTTAAGACCGAATGCGAATCTGACCGTTTATTTATTTGCGCTTTTTAATGAAAATGGGAAAGTGGGACCTACTTCAGAGAGGAATTTTGGGATGTTTTATCCTGATATGAAGAAAGTTTATGATGTCCCGTTTACTGTGGCGGGGTTGAAGAGTTACCGTGACGTTTCGGCGCCGGGAAGCGGCTGA
Protein sequence

>MS.gene25797.t1

MHRLLILSILISSYLSLPLLADGNSIGVNYGRIANNLPSAFKVVKLLKSQGIDRVKLYDTDPAVLKSLSGSGIKVTVNLPNEQLFYTAKKLSYALTWLQKNVVVYHPKTQIEAIAVGNEVFVDTHNTTKYLIPAMKNIHKALIKFNLHNSIKISSPIALSALGSSYPSSTGSFKPDLIEPVIKPMLNFLRETSSYLMVNVYPFFAYESNADVISLNYALFRENPGNVDPGNGLKYYNIFDAQIDAVFAALNKLQYNDVRVVVSETGWPSKGDSDEVGASPQNAAAYNGNLVKKILNNGGTPLRPNANLTVYLFALFNENGKVGPTSERNFGMFYPDMKKVYDVPFTVAGLKSYRDVSAPGSG