AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33571


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33571.t1 MTR_1g103810 89.756 205 15 1 1 199 1 205 5.46e-132 369
MS.gene33571.t1 MTR_1g103810 88.750 160 12 1 1 154 1 160 2.92e-99 285
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33571.t1 AT1G05385 69.231 143 41 1 57 199 6 145 1.77e-64 196
MS.gene33571.t1 AT1G05385 69.231 143 41 1 57 199 60 199 3.94e-64 197

Find 51 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTGACTTTGTCTTCTCAT+TGG 0.218549 1.3:-71134006 None:intergenic
GAAAACGATGATGGGGTTAT+TGG 0.237764 1.3:+71133960 MS.gene33571:CDS
ATCTGCAGTCCGTCTAAATT+TGG 0.273557 1.3:-71134117 None:intergenic
TTACAGTTTATCACCATTAA+TGG 0.273988 1.3:+71133905 MS.gene33571:CDS
TCTTCGGCCCATGCCATTAA+TGG 0.281488 1.3:-71133918 None:intergenic
CCTGACTTTGTCTTCTCATT+GGG 0.285272 1.3:-71134005 None:intergenic
CTAAATTTGGCATTGTCATC+AGG 0.305219 1.3:-71134104 None:intergenic
AAAACGATGATGGGGTTATT+GGG 0.311993 1.3:+71133961 MS.gene33571:CDS
GGTAAAAGCAGTAAGATCTT+TGG 0.364747 1.3:+71134362 MS.gene33571:CDS
AATTAGCGTTGCCACTTCAT+TGG 0.375737 1.3:+71133866 MS.gene33571:CDS
CCCAATGAGAAGACAAAGTC+AGG 0.396652 1.3:+71134005 MS.gene33571:CDS
TGCAGCAAAGGAGTCGATTC+GGG 0.402932 1.3:+71134139 MS.gene33571:CDS
ATCACAAGATTTGACATTGA+AGG 0.411870 1.3:+71133021 MS.gene33571:CDS
GACTTAACTTCCGTCGGCAG+TGG 0.431839 1.3:-71134417 None:intergenic
ATGCAGCAAAGGAGTCGATT+CGG 0.434911 1.3:+71134138 MS.gene33571:CDS
GGAACACATAATAGCTGAAA+AGG 0.441979 1.3:-71132998 None:intergenic
TTTGTCAGCTGACTTCTCTT+CGG 0.445390 1.3:-71133934 None:intergenic
TTCGGGAGTATCTTGGTAGT+TGG 0.447521 1.3:+71134156 MS.gene33571:CDS
AATTGAAAGGTTTCTGCGAC+TGG 0.455585 1.3:-71133847 None:intergenic
GCTGACTTTAAGTAAGCCTT+CGG 0.465034 1.3:-71134038 None:intergenic
GCAGGAATCATACTTTGCTC+TGG 0.488174 1.3:+71134341 MS.gene33571:intron
ACTTAACTTCCGTCGGCAGT+GGG 0.492647 1.3:-71134416 None:intergenic
ATACTTCTTGAGAAACAGTC+TGG 0.498665 1.3:-71134187 None:intergenic
TCCGTCGGCAGTGGGGCTGA+TGG 0.499406 1.3:-71134408 None:intergenic
TAACAGATAAACTGCAATCC+AGG 0.501310 1.3:+71133793 MS.gene33571:intron
GACAAAGAGGAAAACGATGA+TGG 0.503005 1.3:+71133951 MS.gene33571:CDS
GGGAGTATCTTGGTAGTTGG+AGG 0.505346 1.3:+71134159 MS.gene33571:CDS
GGGATCAAATAACGATTTGA+CGG 0.517749 1.3:-71133985 None:intergenic
GAGTCGATTCGGGAGTATCT+TGG 0.529772 1.3:+71134149 MS.gene33571:CDS
ACAAAGAGGAAAACGATGAT+GGG 0.531382 1.3:+71133952 MS.gene33571:CDS
GTTTATCACCATTAATGGCA+TGG 0.531611 1.3:+71133910 MS.gene33571:CDS
TAAATTTGGCATTGTCATCA+GGG 0.539047 1.3:-71134103 None:intergenic
TTCAATGTCAAATCTTGTGA+TGG 0.542981 1.3:-71133019 None:intergenic
TTTATCACCATTAATGGCAT+GGG 0.553461 1.3:+71133911 MS.gene33571:CDS
GCCATCAGCCCCACTGCCGA+CGG 0.555502 1.3:+71134407 MS.gene33571:CDS
TTACTTAAAGTCAGCAAGAG+AGG 0.560221 1.3:+71134046 MS.gene33571:CDS
AAGTGGCAACGCTAATTGAA+AGG 0.563857 1.3:-71133860 None:intergenic
AGAGAAGTCAGCTGACAAAG+AGG 0.564571 1.3:+71133938 MS.gene33571:CDS
TGTTATTGTAACCAATGAAG+TGG 0.566510 1.3:-71133877 None:intergenic
AAACGATGATGGGGTTATTG+GGG 0.568617 1.3:+71133962 MS.gene33571:CDS
GGAGTATCTTGGTAGTTGGA+GGG 0.581542 1.3:+71134160 MS.gene33571:CDS
CTGCGACTGGAAGCTTCGAG+AGG 0.595865 1.3:-71133834 None:intergenic
ACGGACTGCAGATGCAGCAA+AGG 0.596536 1.3:+71134127 MS.gene33571:CDS
TCATTGGTTACAATAACATG+TGG 0.609111 1.3:+71133882 MS.gene33571:CDS
AAGCTGTGCAGTATTAGACC+TGG 0.611400 1.3:-71133811 None:intergenic
ATGACAATGCCAAATTTAGA+CGG 0.613681 1.3:+71134108 MS.gene33571:CDS
ATTTCAGACTTAACTTCCGT+CGG 0.625733 1.3:-71134423 None:intergenic
CTTAACTTCCGTCGGCAGTG+GGG 0.642851 1.3:-71134415 None:intergenic
CAAAGAGGAAAACGATGATG+GGG 0.655727 1.3:+71133953 MS.gene33571:CDS
GAGTATCTTGGTAGTTGGAG+GGG 0.662716 1.3:+71134161 MS.gene33571:CDS
CCAATGAGAAGACAAAGTCA+GGG 0.666576 1.3:+71134006 MS.gene33571:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATATATAATCATGCTT+TGG + chr1.3:71134307-71134326 MS.gene33571:intron 15.0%
!! ATTAATCTATTTATCCAAAT+AGG + chr1.3:71133299-71133318 MS.gene33571:intron 15.0%
!! AAACTGTTATGTATAGTTTA+TGG - chr1.3:71133555-71133574 None:intergenic 20.0%
!!! AAATTGTTTTAAGGTTTGTT+TGG + chr1.3:71133421-71133440 MS.gene33571:intron 20.0%
!!! TAAGCTGTTTTTTATATCAA+AGG - chr1.3:71133626-71133645 None:intergenic 20.0%
!!! TATTTCAAACATTTTGAGAT+TGG + chr1.3:71133055-71133074 MS.gene33571:intron 20.0%
!!! TCAAAATGTTTGAAATAGTA+AGG - chr1.3:71133052-71133071 None:intergenic 20.0%
! ATATGCACTTATATGATAAG+GGG + chr1.3:71133649-71133668 MS.gene33571:intron 25.0%
! ATTGAATGATACAACCTATT+TGG - chr1.3:71133316-71133335 None:intergenic 25.0%
! CATATGCACTTATATGATAA+GGG + chr1.3:71133648-71133667 MS.gene33571:intron 25.0%
! TTACAGTTTATCACCATTAA+TGG + chr1.3:71133905-71133924 MS.gene33571:CDS 25.0%
!! TAGCATATATGTTAGGATTT+TGG + chr1.3:71134254-71134273 MS.gene33571:intron 25.0%
!!! TTTTAAGGTTTGTTTGGAAT+TGG + chr1.3:71133427-71133446 MS.gene33571:intron 25.0%
ATCACAAGATTTGACATTGA+AGG + chr1.3:71133021-71133040 MS.gene33571:CDS 30.0%
ATGACAATGCCAAATTTAGA+CGG + chr1.3:71134108-71134127 MS.gene33571:CDS 30.0%
ATGGTAAACTAAGTAAGAGT+AGG + chr1.3:71133739-71133758 MS.gene33571:intron 30.0%
CATTCACAAAAAGAAAAGAC+TGG - chr1.3:71134286-71134305 None:intergenic 30.0%
CTTTGACTAGCATATATGTT+AGG + chr1.3:71134247-71134266 MS.gene33571:intron 30.0%
GAAAAGCTGTCAATAAATGA+AGG - chr1.3:71133766-71133785 None:intergenic 30.0%
GCATATGCACTTATATGATA+AGG + chr1.3:71133647-71133666 MS.gene33571:intron 30.0%
GGTTGTATCATTCAATTGAA+AGG + chr1.3:71133320-71133339 MS.gene33571:intron 30.0%
GTCATACAATACTTACAACA+TGG + chr1.3:71133720-71133739 MS.gene33571:intron 30.0%
TAAATTTGGCATTGTCATCA+GGG - chr1.3:71134106-71134125 None:intergenic 30.0%
TGCTTATTATGAGAGAACTA+TGG + chr1.3:71133463-71133482 MS.gene33571:intron 30.0%
TGTTATTGTAACCAATGAAG+TGG - chr1.3:71133880-71133899 None:intergenic 30.0%
TTCAATGTCAAATCTTGTGA+TGG - chr1.3:71133022-71133041 None:intergenic 30.0%
TTTATCACCATTAATGGCAT+GGG + chr1.3:71133911-71133930 MS.gene33571:CDS 30.0%
! GTAAGTATTGTATGACACAA+AGG - chr1.3:71133716-71133735 None:intergenic 30.0%
! TCATTGGTTACAATAACATG+TGG + chr1.3:71133882-71133901 MS.gene33571:CDS 30.0%
!! TTGCACTATAGTATTGATAG+TGG + chr1.3:71133393-71133412 MS.gene33571:intron 30.0%
ACAAAGAGGAAAACGATGAT+GGG + chr1.3:71133952-71133971 MS.gene33571:CDS 35.0%
ATACTTCTTGAGAAACAGTC+TGG - chr1.3:71134190-71134209 None:intergenic 35.0%
ATTTCAGACTTAACTTCCGT+CGG - chr1.3:71134426-71134445 None:intergenic 35.0%
CTAAATTTGGCATTGTCATC+AGG - chr1.3:71134107-71134126 None:intergenic 35.0%
GGAACACATAATAGCTGAAA+AGG - chr1.3:71133001-71133020 None:intergenic 35.0%
GGGATCAAATAACGATTTGA+CGG - chr1.3:71133988-71134007 None:intergenic 35.0%
GGTAAAAGCAGTAAGATCTT+TGG + chr1.3:71134362-71134381 MS.gene33571:CDS 35.0%
GTTTATCACCATTAATGGCA+TGG + chr1.3:71133910-71133929 MS.gene33571:CDS 35.0%
TAACAGATAAACTGCAATCC+AGG + chr1.3:71133793-71133812 MS.gene33571:intron 35.0%
TTACTTAAAGTCAGCAAGAG+AGG + chr1.3:71134046-71134065 MS.gene33571:CDS 35.0%
! TTTGGCAAACTTTTACTCAG+AGG + chr1.3:71134380-71134399 MS.gene33571:CDS 35.0%
!! AAAACGATGATGGGGTTATT+GGG + chr1.3:71133961-71133980 MS.gene33571:CDS 35.0%
!! GTGGCTCGTAAATTGTTTTA+AGG + chr1.3:71133412-71133431 MS.gene33571:intron 35.0%
AAGTGGCAACGCTAATTGAA+AGG - chr1.3:71133863-71133882 None:intergenic 40.0%
AATTGAAAGGTTTCTGCGAC+TGG - chr1.3:71133850-71133869 None:intergenic 40.0%
AGCAAAGTATGATTCCTGCA+AGG - chr1.3:71134340-71134359 None:intergenic 40.0%
ATCTGCAGTCCGTCTAAATT+TGG - chr1.3:71134120-71134139 None:intergenic 40.0%
CAAAGAGGAAAACGATGATG+GGG + chr1.3:71133953-71133972 MS.gene33571:CDS 40.0%
CCAATGAGAAGACAAAGTCA+GGG + chr1.3:71134006-71134025 MS.gene33571:CDS 40.0%
CCTGACTTTGTCTTCTCATT+GGG - chr1.3:71134008-71134027 None:intergenic 40.0%
GACAAAGAGGAAAACGATGA+TGG + chr1.3:71133951-71133970 MS.gene33571:CDS 40.0%
GCAAAGTATGATTCCTGCAA+GGG - chr1.3:71134339-71134358 None:intergenic 40.0%
TATGACACAAAGGCTCTGTT+TGG - chr1.3:71133706-71133725 None:intergenic 40.0%
! AATTAGCGTTGCCACTTCAT+TGG + chr1.3:71133866-71133885 MS.gene33571:CDS 40.0%
! CAAACTTTTACTCAGAGGCA+GGG + chr1.3:71134385-71134404 MS.gene33571:CDS 40.0%
! GCTGACTTTAAGTAAGCCTT+CGG - chr1.3:71134041-71134060 None:intergenic 40.0%
! TTTGTCAGCTGACTTCTCTT+CGG - chr1.3:71133937-71133956 None:intergenic 40.0%
!! AAACGATGATGGGGTTATTG+GGG + chr1.3:71133962-71133981 MS.gene33571:CDS 40.0%
!! GAAAACGATGATGGGGTTAT+TGG + chr1.3:71133960-71133979 MS.gene33571:CDS 40.0%
AAGCTGTGCAGTATTAGACC+TGG - chr1.3:71133814-71133833 None:intergenic 45.0%
AGAGAAGTCAGCTGACAAAG+AGG + chr1.3:71133938-71133957 MS.gene33571:CDS 45.0%
ATGCAGCAAAGGAGTCGATT+CGG + chr1.3:71134138-71134157 MS.gene33571:CDS 45.0%
CCCAATGAGAAGACAAAGTC+AGG + chr1.3:71134005-71134024 MS.gene33571:CDS 45.0%
CCCTGACTTTGTCTTCTCAT+TGG - chr1.3:71134009-71134028 None:intergenic 45.0%
GCAGGAATCATACTTTGCTC+TGG + chr1.3:71134341-71134360 MS.gene33571:intron 45.0%
! GAGTATCTTGGTAGTTGGAG+GGG + chr1.3:71134161-71134180 MS.gene33571:CDS 45.0%
! GCAAACTTTTACTCAGAGGC+AGG + chr1.3:71134384-71134403 MS.gene33571:CDS 45.0%
! GGAGTATCTTGGTAGTTGGA+GGG + chr1.3:71134160-71134179 MS.gene33571:CDS 45.0%
!! TTCGGGAGTATCTTGGTAGT+TGG + chr1.3:71134156-71134175 MS.gene33571:CDS 45.0%
ACTTAACTTCCGTCGGCAGT+GGG - chr1.3:71134419-71134438 None:intergenic 50.0%
TCTTCGGCCCATGCCATTAA+TGG - chr1.3:71133921-71133940 None:intergenic 50.0%
! GCTTTGGATTGATCCCTTGC+AGG + chr1.3:71134323-71134342 MS.gene33571:intron 50.0%
! GTCAGGGAAAGTTTTGCCGA+AGG + chr1.3:71134022-71134041 MS.gene33571:CDS 50.0%
! TGCAGCAAAGGAGTCGATTC+GGG + chr1.3:71134139-71134158 MS.gene33571:CDS 50.0%
!! GAGTCGATTCGGGAGTATCT+TGG + chr1.3:71134149-71134168 MS.gene33571:CDS 50.0%
!! GGGAGTATCTTGGTAGTTGG+AGG + chr1.3:71134159-71134178 MS.gene33571:CDS 50.0%
CTTAACTTCCGTCGGCAGTG+GGG - chr1.3:71134418-71134437 None:intergenic 55.0%
GACTTAACTTCCGTCGGCAG+TGG - chr1.3:71134420-71134439 None:intergenic 55.0%
! ACGGACTGCAGATGCAGCAA+AGG + chr1.3:71134127-71134146 MS.gene33571:CDS 55.0%
CTGCGACTGGAAGCTTCGAG+AGG - chr1.3:71133837-71133856 None:intergenic 60.0%
GCCATCAGCCCCACTGCCGA+CGG + chr1.3:71134407-71134426 MS.gene33571:CDS 70.0%
TCCGTCGGCAGTGGGGCTGA+TGG - chr1.3:71134411-71134430 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 71132988 71134482 71132988 ID=MS.gene33571
chr1.3 mRNA 71132988 71134482 71132988 ID=MS.gene33571.t1;Parent=MS.gene33571
chr1.3 exon 71132988 71133042 71132988 ID=MS.gene33571.t1.exon1;Parent=MS.gene33571.t1
chr1.3 CDS 71132988 71133042 71132988 ID=cds.MS.gene33571.t1;Parent=MS.gene33571.t1
chr1.3 exon 71133799 71134205 71133799 ID=MS.gene33571.t1.exon2;Parent=MS.gene33571.t1
chr1.3 CDS 71133799 71134205 71133799 ID=cds.MS.gene33571.t1;Parent=MS.gene33571.t1
chr1.3 exon 71134345 71134482 71134345 ID=MS.gene33571.t1.exon3;Parent=MS.gene33571.t1
chr1.3 CDS 71134345 71134482 71134345 ID=cds.MS.gene33571.t1;Parent=MS.gene33571.t1
Gene Sequence

>MS.gene33571

ATGGCAGCTACCTTTTCAGCTATTATGTGTTCCATCACAAGATTTGACATTGAAGGTGTGTCCTTACTATTTCAAACATTTTGAGATTGGCTTCTTTACTCTCATAATCACTTGTGTGACTGTTTGTGAGAACTTATGACATGCTCATAAGCTGTTTGTTTTCAATTTATTTTTGTAAGTTCATCATGATAGCTTATGAAAACAACTTATAGTTCATACGAAAATAATTTATTTAACTTTAGTTTATCTTTTGTTATACAAATAGTTTATACATAAGCGCTTATGATAAATATTAATACTATAAACTGCAAATTAATCTATTTATCCAAATAGGTTGTATCATTCAATTGAAAGGAAAGAAAGTAGAATAAATGTTATCATTCGAGAGTCGACAAGACAACATATTTGCACTATAGTATTGATAGTGGCTCGTAAATTGTTTTAAGGTTTGTTTGGAATTGGATTAACTTATTTATGCTTATTATGAGAGAACTATGGAACAACTTATGAGTCATGTCTATAAGCTTCTTTTTTTTTTTTTTTTTTTTTTTTGCTTTCTTATTTCCATAAACTATACATAACAGTTTATGAAAACAATTTATAACTTATATGAAAACAGTTTGTCTTTATATTATCCTTTGATATAAAAAACAGCTTATGCATATGCACTTATATGATAAGGGGTATGCTATAAGTACTTAATGAAGTTGTTTATCCAAACAGAGCCTTTGTGTCATACAATACTTACAACATGGTAAACTAAGTAAGAGTAGGTCCTTCATTTATTGACAGCTTTTCTACAATATAACAGATAAACTGCAATCCAGGTCTAATACTGCACAGCTTCCTCTCGAAGCTTCCAGTCGCAGAAACCTTTCAATTAGCGTTGCCACTTCATTGGTTACAATAACATGTGGTTACAGTTTATCACCATTAATGGCATGGGCCGAAGAGAAGTCAGCTGACAAAGAGGAAAACGATGATGGGGTTATTGGGGCCGTCAAATCGTTATTTGATCCCAATGAGAAGACAAAGTCAGGGAAAGTTTTGCCGAAGGCTTACTTAAAGTCAGCAAGAGAGGTAGTGAAGACACTGCGTGAATCTTTAAATGAAGACCCTGATGACAATGCCAAATTTAGACGGACTGCAGATGCAGCAAAGGAGTCGATTCGGGAGTATCTTGGTAGTTGGAGGGGAAACCAGACTGTTTCTCAAGAAGTATGTACTTTTGACTAAATTTAAGCAATGAAAATTCAATGCTTTGACTAGCATATATGTTAGGATTTTGGTGTATGCCAGTCTTTTCTTTTTGTGAATGCAAATATATATAATCATGCTTTGGATTGATCCCTTGCAGGAATCATACTTTGCTCTGGTAAAAGCAGTAAGATCTTTGGCAAACTTTTACTCAGAGGCAGGGCCATCAGCCCCACTGCCGACGGAAGTTAAGTCTGAAATATTAAATTATCTGAATACCGCTGAAGAATTTTTGTAG

Protein sequence

>MS.gene33571.t1

MAATFSAIMCSITRFDIEDKLQSRSNTAQLPLEASSRRNLSISVATSLVTITCGYSLSPLMAWAEEKSADKEENDDGVIGAVKSLFDPNEKTKSGKVLPKAYLKSAREVVKTLRESLNEDPDDNAKFRRTADAAKESIREYLGSWRGNQTVSQEESYFALVKAVRSLANFYSEAGPSAPLPTEVKSEILNYLNTAEEFL