AlfalfaGEDB | MS.gene35499

M. sativa cultivar XinJiangDaYe / MS.gene35499

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12937.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35499.t1	XP_024631582.1	86.3	190	15	1	1	190	1	179	8.70E-58	233.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35499.t1	Q9LUP9	34.2	76	50	0	6	81	3	78	5.4e-08	59.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35499.t1	A2Q247	86.3	190	15	1	1	190	1	179	6.3e-58	233.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049129	MS.gene35499	-0.819388	1.19E-52	-1.69E-46
MS.gene049580	MS.gene35499	0.812032	5.20E-51	-1.69E-46
MS.gene050752	MS.gene35499	0.862483	4.91E-64	-1.69E-46
MS.gene050838	MS.gene35499	0.805585	1.24E-49	-1.69E-46
MS.gene050945	MS.gene35499	0.810457	1.14E-50	-1.69E-46
MS.gene050955	MS.gene35499	0.857251	1.85E-62	-1.69E-46
MS.gene051003	MS.gene35499	0.861331	1.11E-63	-1.69E-46
MS.gene051234	MS.gene35499	0.818659	1.75E-52	-1.69E-46
MS.gene051343	MS.gene35499	0.832659	8.33E-56	-1.69E-46
MS.gene05188	MS.gene35499	0.813285	2.77E-51	-1.69E-46
MS.gene051902	MS.gene35499	0.819711	1.01E-52	-1.69E-46
MS.gene051903	MS.gene35499	0.821083	4.87E-53	-1.69E-46
MS.gene051975	MS.gene35499	0.818753	1.66E-52	-1.69E-46
MS.gene052072	MS.gene35499	0.812909	3.34E-51	-1.69E-46
MS.gene052947	MS.gene35499	0.83079	2.41E-55	-1.69E-46
MS.gene053058	MS.gene35499	0.817764	2.78E-52	-1.69E-46
MS.gene05364	MS.gene35499	0.806802	6.89E-50	-1.69E-46
MS.gene054004	MS.gene35499	0.811292	7.53E-51	-1.69E-46
MS.gene054403	MS.gene35499	0.867859	1.00E-65	-1.69E-46
MS.gene05469	MS.gene35499	0.819656	1.04E-52	-1.69E-46
MS.gene055555	MS.gene35499	0.83735	5.48E-57	-1.69E-46
MS.gene056986	MS.gene35499	0.822862	1.89E-53	-1.69E-46
MS.gene057102	MS.gene35499	0.833737	4.49E-56	-1.69E-46
MS.gene057705	MS.gene35499	0.841775	3.87E-58	-1.69E-46
MS.gene05781	MS.gene35499	0.824128	9.55E-54	-1.69E-46
MS.gene057890	MS.gene35499	-0.800965	1.13E-48	-1.69E-46
MS.gene057999	MS.gene35499	0.811058	8.46E-51	-1.69E-46
MS.gene058238	MS.gene35499	0.808216	3.45E-50	-1.69E-46
MS.gene058708	MS.gene35499	-0.800557	1.36E-48	-1.69E-46
MS.gene059146	MS.gene35499	0.836469	9.19E-57	-1.69E-46
MS.gene059227	MS.gene35499	0.82294	1.81E-53	-1.69E-46
MS.gene05985	MS.gene35499	-0.827805	1.28E-54	-1.69E-46
MS.gene059901	MS.gene35499	-0.818165	2.26E-52	-1.69E-46
MS.gene06011	MS.gene35499	0.819414	1.18E-52	-1.69E-46
MS.gene060244	MS.gene35499	0.807491	4.93E-50	-1.69E-46
MS.gene060365	MS.gene35499	0.801758	7.74E-49	-1.69E-46
MS.gene060763	MS.gene35499	0.81583	7.56E-52	-1.69E-46
MS.gene061100	MS.gene35499	0.823252	1.53E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35499.t1	MTR_2g021600	83.684	190	20	1	1	190	1	179	1.76e-100	290
MS.gene35499.t1	MTR_2g021630	40.741	189	81	3	3	191	2	159	1.35e-32	115
MS.gene35499.t1	MTR_2g020500	55.769	104	45	1	3	106	13	115	2.85e-31	114
MS.gene35499.t1	MTR_2g021480	41.089	202	96	5	1	185	11	206	8.97e-29	108
MS.gene35499.t1	MTR_2g021560	57.778	90	37	1	3	92	2	90	1.71e-26	102
MS.gene35499.t1	MTR_2g021640	55.682	88	38	1	3	90	32	118	5.74e-25	96.7
MS.gene35499.t1	MTR_2g021510	36.458	192	85	6	4	191	13	171	1.12e-23	92.4
MS.gene35499.t1	MTR_0485s0020	37.433	187	109	3	4	190	3	181	1.57e-23	92.4
MS.gene35499.t1	MTR_2g021530	54.667	75	33	1	7	81	2	75	2.40e-20	82.4
MS.gene35499.t1	MTR_1g025830	31.513	238	96	7	3	189	2	223	4.21e-20	84.3
MS.gene35499.t1	MTR_2g021450	35.165	182	80	5	3	184	2	145	1.78e-19	80.9
MS.gene35499.t1	MTR_2g021660	53.333	75	34	1	1	75	1	74	3.99e-18	78.2
MS.gene35499.t1	MTR_2g021590	50.649	77	37	1	1	77	4	79	3.40e-17	77.0
MS.gene35499.t1	MTR_2g021430	33.511	188	79	7	3	190	2	143	1.31e-16	73.2
MS.gene35499.t1	MTR_3g087310	49.333	75	37	1	3	77	2	75	2.01e-16	70.9
MS.gene35499.t1	MTR_2g021570	60.000	60	24	0	14	73	34	93	2.83e-16	71.2
MS.gene35499.t1	MTR_1g048260	49.351	77	34	1	3	79	2	73	4.25e-16	73.6
MS.gene35499.t1	MTR_2g022750	34.409	186	110	4	9	189	11	189	1.31e-14	69.3
MS.gene35499.t1	MTR_2g022750	34.409	186	110	4	9	189	11	189	1.63e-14	68.9
MS.gene35499.t1	MTR_5g048270	26.724	232	118	6	7	191	2	228	1.78e-14	69.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 38 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTGCTTCTTAAGGAATTT+AGG	0.194647	2.4:-65673665	None:intergenic
TTCTGACGTGGATAATCTTT+TGG	0.310646	2.4:+65673949	MS.gene35499:CDS
ATCCTCCAGTGCTTGGGATT+GGG	0.342543	2.4:-65674026	None:intergenic
TATCCTCCAGTGCTTGGGAT+TGG	0.354112	2.4:-65674027	None:intergenic
TCCGGAAGAACCACCGTGTT+TGG	0.360103	2.4:-65673548	None:intergenic
CGAATTGAAACGGGTGTTGA+TGG	0.362790	2.4:+65673919	MS.gene35499:CDS
CGTTTGCAAACGATGGAATT+CGG	0.377067	2.4:+65673631	MS.gene35499:CDS
AGATCTAACCTCCAAGGCTT+TGG	0.383571	2.4:+65673712	MS.gene35499:CDS
ATTTCGATTATCAGTTCTTC+CGG	0.390404	2.4:-65673566	None:intergenic
CATTATATCCTCCAGTGCTT+GGG	0.417270	2.4:-65674032	None:intergenic
TCATTATATCCTCCAGTGCT+TGG	0.419753	2.4:-65674033	None:intergenic
ACCAAACACGGTGGTTCTTC+CGG	0.427304	2.4:+65673547	MS.gene35499:CDS
TTCTTCCTCCTCTTGAGGCA+TGG	0.443136	2.4:-65673780	None:intergenic
GAATTCCATCGTTTGCAAAC+GGG	0.443646	2.4:-65673629	None:intergenic
TCATTTCCAGAGTTGATTCA+AGG	0.453871	2.4:+65673588	MS.gene35499:CDS
AGCTCTGAAAGTGCTTCTTA+AGG	0.482555	2.4:-65673673	None:intergenic
CGAATTCCATCGTTTGCAAA+CGG	0.485859	2.4:-65673630	None:intergenic
AGCAATGACGGCGGAAGCAA+CGG	0.488849	2.4:+65673517	None:intergenic
GCACTTTCAGAGCTTGTTCA+CGG	0.493716	2.4:+65673682	MS.gene35499:CDS
GGACTGTTCCAAAGCCTTGG+AGG	0.494324	2.4:-65673720	None:intergenic
TCTTCTTCTTCCTCCTCTTG+AGG	0.507183	2.4:-65673785	None:intergenic
ATTGGACTGTTCCAAAGCCT+TGG	0.508501	2.4:-65673723	None:intergenic
AGTATTCCTTGAATCAACTC+TGG	0.529325	2.4:-65673594	None:intergenic
GCAAACCCAATCCCAAGCAC+TGG	0.551500	2.4:+65674021	MS.gene35499:CDS
CGCGGCCATGCCTCAAGAGG+AGG	0.572319	2.4:+65673775	MS.gene35499:CDS
CAAGGAATACTCTGCAACTG+AGG	0.574910	2.4:+65673606	MS.gene35499:CDS
AACCCAATCCCAAGCACTGG+AGG	0.597028	2.4:+65674024	MS.gene35499:CDS
TGAGGCCCGTTTGCAAACGA+TGG	0.616269	2.4:+65673624	MS.gene35499:CDS
TAACGCGGCCATGCCTCAAG+AGG	0.620807	2.4:+65673772	MS.gene35499:CDS
AACGGCGGAGTCACCAAACA+CGG	0.632733	2.4:+65673535	MS.gene35499:CDS
ATGTTGCGATATGAAAGCAT+TGG	0.637121	2.4:-65673741	None:intergenic
AATGACGGCGGAAGCAACGG+CGG	0.640542	2.4:+65673520	None:intergenic
CATCAAAGATCTAACCTCCA+AGG	0.641038	2.4:+65673706	MS.gene35499:CDS
GATGGATTTAGTTTCTGACG+TGG	0.707895	2.4:+65673937	MS.gene35499:CDS
CTTAACGAGAGCAATGACGG+CGG	0.742276	2.4:+65673508	None:intergenic
ATTGGGTTTGCATATCAACG+TGG	0.746293	2.4:-65674009	None:intergenic
GCAACATATTATAAATAACG+CGG	0.768241	2.4:+65673757	MS.gene35499:CDS
GGCGGAGTCACCAAACACGG+TGG	0.780105	2.4:+65673538	MS.gene35499:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGAAAAAGACGAATTGAAA+CGG	+	chr2.4:65673909-65673928	MS.gene35499:CDS	25.0%
!	GCAACATATTATAAATAACG+CGG	+	chr2.4:65673757-65673776	MS.gene35499:CDS	25.0%
!!	ATAATCTTTTGGTGAATCTT+CGG	+	chr2.4:65673960-65673979	MS.gene35499:CDS	25.0%
	AAGTGCTTCTTAAGGAATTT+AGG	-	chr2.4:65673668-65673687	None:intergenic	30.0%
	AGAAAAAGACGAATTGAAAC+GGG	+	chr2.4:65673910-65673929	MS.gene35499:CDS	30.0%
	ATTTCGATTATCAGTTCTTC+CGG	-	chr2.4:65673569-65673588	None:intergenic	30.0%
	AGTATTCCTTGAATCAACTC+TGG	-	chr2.4:65673597-65673616	None:intergenic	35.0%
!	ATGTTGCGATATGAAAGCAT+TGG	-	chr2.4:65673744-65673763	None:intergenic	35.0%
!	TCATTTCCAGAGTTGATTCA+AGG	+	chr2.4:65673588-65673607	MS.gene35499:CDS	35.0%
!	TTCTGACGTGGATAATCTTT+TGG	+	chr2.4:65673949-65673968	MS.gene35499:CDS	35.0%
	ATTGGGTTTGCATATCAACG+TGG	-	chr2.4:65674012-65674031	None:intergenic	40.0%
	CATCAAAGATCTAACCTCCA+AGG	+	chr2.4:65673706-65673725	MS.gene35499:CDS	40.0%
	CATTATATCCTCCAGTGCTT+GGG	-	chr2.4:65674035-65674054	None:intergenic	40.0%
	CGAATTCCATCGTTTGCAAA+CGG	-	chr2.4:65673633-65673652	None:intergenic	40.0%
	CGTTTGCAAACGATGGAATT+CGG	+	chr2.4:65673631-65673650	MS.gene35499:CDS	40.0%
	GAATTCCATCGTTTGCAAAC+GGG	-	chr2.4:65673632-65673651	None:intergenic	40.0%
	TCATTATATCCTCCAGTGCT+TGG	-	chr2.4:65674036-65674055	None:intergenic	40.0%
!	GATGGATTTAGTTTCTGACG+TGG	+	chr2.4:65673937-65673956	MS.gene35499:CDS	40.0%
!!	AGCTCTGAAAGTGCTTCTTA+AGG	-	chr2.4:65673676-65673695	None:intergenic	40.0%
	AGATCTAACCTCCAAGGCTT+TGG	+	chr2.4:65673712-65673731	MS.gene35499:CDS	45.0%
	ATTGGACTGTTCCAAAGCCT+TGG	-	chr2.4:65673726-65673745	None:intergenic	45.0%
	CAAGGAATACTCTGCAACTG+AGG	+	chr2.4:65673606-65673625	MS.gene35499:CDS	45.0%
	TCTTCTTCTTCCTCCTCTTG+AGG	-	chr2.4:65673788-65673807	None:intergenic	45.0%
!	GCACTTTCAGAGCTTGTTCA+CGG	+	chr2.4:65673682-65673701	MS.gene35499:CDS	45.0%
!!	CGAATTGAAACGGGTGTTGA+TGG	+	chr2.4:65673919-65673938	MS.gene35499:CDS	45.0%
	ACCAAACACGGTGGTTCTTC+CGG	+	chr2.4:65673547-65673566	MS.gene35499:CDS	50.0%
	ATCCTCCAGTGCTTGGGATT+GGG	-	chr2.4:65674029-65674048	None:intergenic	50.0%
	TATCCTCCAGTGCTTGGGAT+TGG	-	chr2.4:65674030-65674049	None:intergenic	50.0%
	TTCTTCCTCCTCTTGAGGCA+TGG	-	chr2.4:65673783-65673802	None:intergenic	50.0%
	AACGGCGGAGTCACCAAACA+CGG	+	chr2.4:65673535-65673554	MS.gene35499:CDS	55.0%
	GCAAACCCAATCCCAAGCAC+TGG	+	chr2.4:65674021-65674040	MS.gene35499:CDS	55.0%
	GGACTGTTCCAAAGCCTTGG+AGG	-	chr2.4:65673723-65673742	None:intergenic	55.0%
	TCCGGAAGAACCACCGTGTT+TGG	-	chr2.4:65673551-65673570	None:intergenic	55.0%
	TGAGGCCCGTTTGCAAACGA+TGG	+	chr2.4:65673624-65673643	MS.gene35499:CDS	55.0%
!!	AACCCAATCCCAAGCACTGG+AGG	+	chr2.4:65674024-65674043	MS.gene35499:CDS	55.0%
	TAACGCGGCCATGCCTCAAG+AGG	+	chr2.4:65673772-65673791	MS.gene35499:CDS	60.0%
	GGCGGAGTCACCAAACACGG+TGG	+	chr2.4:65673538-65673557	MS.gene35499:CDS	65.0%
	CGCGGCCATGCCTCAAGAGG+AGG	+	chr2.4:65673775-65673794	MS.gene35499:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	65673521	65674096	65673521	ID=MS.gene35499
chr2.4	mRNA	65673521	65674096	65673521	ID=MS.gene35499.t1;Parent=MS.gene35499
chr2.4	exon	65673521	65674096	65673521	ID=MS.gene35499.t1.exon1;Parent=MS.gene35499.t1
chr2.4	CDS	65673521	65674096	65673521	ID=cds.MS.gene35499.t1;Parent=MS.gene35499.t1

Gene Sequence

>MS.gene35499

ATGACGGCGGAAGCAACGGCGGAGTCACCAAACACGGTGGTTCTTCCGGAAGAACTGATAATCGAAATCATTTCCAGAGTTGATTCAAGGAATACTCTGCAACTGAGGCCCGTTTGCAAACGATGGAATTCGGTTATTACTGATCCTAAATTCCTTAAGAAGCACTTTCAGAGCTTGTTCACGGACATCAAAGATCTAACCTCCAAGGCTTTGGAACAGTCCAATGCTTTCATATCGCAACATATTATAAATAACGCGGCCATGCCTCAAGAGGAGGAAGAAGAAGATGCTGAAGAAGAAGAAGAAGAAGATGCTGCTGCTGTTGTTGAAGAAGAAGATGTTGTTGTTGCTGCTGTTGATGAAGAAGAAGATGTTGCTGCTGTTGATGAAGAAAAAGACGAATTGAAACGGGTGTTGATGGATTTAGTTTCTGACGTGGATAATCTTTTGGTGAATCTTCGGTTTATCAAAGACAATACAGAAACTATCCACGTTGATATGCAAACCCAATCCCAAGCACTGGAGGATATAATGAAGTGTTTTCGAAGCTTTGTACGAATTTATCTCAAGTCATAA

Protein sequence

>MS.gene35499.t1

MTAEATAESPNTVVLPEELIIEIISRVDSRNTLQLRPVCKRWNSVITDPKFLKKHFQSLFTDIKDLTSKALEQSNAFISQHIINNAAMPQEEEEEDAEEEEEEDAAAVVEEEDVVVAAVDEEEDVAAVDEEKDELKRVLMDLVSDVDNLLVNLRFIKDNTETIHVDMQTQSQALEDIMKCFRSFVRIYLKS