AlfalfaGEDB | MS.gene40087

M. sativa cultivar XinJiangDaYe / MS.gene40087

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00439.28 PF17035.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40087.t1	XP_003608349.1	94.4	483	27	0	1	483	1	483	6.30E-223	783.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40087.t1	Q7Y214	42.4	543	238	10	1	483	1	528	1.0e-72	275.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40087.t1	A0A072UP94	94.4	483	27	0	1	483	1	483	4.5e-223	783.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene40087	0.81786	2.65E-52	-1.69E-46
MS.gene049807	MS.gene40087	0.802862	4.59E-49	-1.69E-46
MS.gene050359	MS.gene40087	0.818348	2.05E-52	-1.69E-46
MS.gene050414	MS.gene40087	0.858896	6.01E-63	-1.69E-46
MS.gene050646	MS.gene40087	0.800191	1.62E-48	-1.69E-46
MS.gene050654	MS.gene40087	0.80986	1.54E-50	-1.69E-46
MS.gene051043	MS.gene40087	0.810502	1.12E-50	-1.69E-46
MS.gene051852	MS.gene40087	0.813149	2.96E-51	-1.69E-46
MS.gene052016	MS.gene40087	0.850671	1.46E-60	-1.69E-46
MS.gene052052	MS.gene40087	0.812448	4.22E-51	-1.69E-46
MS.gene052307	MS.gene40087	0.846422	2.19E-59	-1.69E-46
MS.gene052463	MS.gene40087	0.836496	9.05E-57	-1.69E-46
MS.gene052493	MS.gene40087	0.809925	1.49E-50	-1.69E-46
MS.gene052533	MS.gene40087	0.813707	2.23E-51	-1.69E-46
MS.gene052609	MS.gene40087	0.82907	6.32E-55	-1.69E-46
MS.gene052775	MS.gene40087	0.801326	9.49E-49	-1.69E-46
MS.gene052776	MS.gene40087	0.815966	7.05E-52	-1.69E-46
MS.gene052932	MS.gene40087	0.821059	4.94E-53	-1.69E-46
MS.gene053079	MS.gene40087	0.837601	4.72E-57	-1.69E-46
MS.gene053143	MS.gene40087	0.810221	1.28E-50	-1.69E-46
MS.gene053618	MS.gene40087	0.824048	9.97E-54	-1.69E-46
MS.gene053620	MS.gene40087	0.8703	1.62E-66	-1.69E-46
MS.gene054178	MS.gene40087	0.83073	2.49E-55	-1.69E-46
MS.gene054222	MS.gene40087	0.800652	1.30E-48	-1.69E-46
MS.gene054641	MS.gene40087	0.808025	3.79E-50	-1.69E-46
MS.gene054892	MS.gene40087	0.854937	8.82E-62	-1.69E-46
MS.gene054893	MS.gene40087	0.855977	4.39E-62	-1.69E-46
MS.gene05539	MS.gene40087	0.818843	1.59E-52	-1.69E-46
MS.gene055469	MS.gene40087	0.800782	1.23E-48	-1.69E-46
MS.gene055513	MS.gene40087	0.854099	1.54E-61	-1.69E-46
MS.gene055864	MS.gene40087	0.86117	1.24E-63	-1.69E-46
MS.gene056045	MS.gene40087	0.817278	3.58E-52	-1.69E-46
MS.gene056285	MS.gene40087	0.811721	6.08E-51	-1.69E-46
MS.gene056507	MS.gene40087	0.808384	3.18E-50	-1.69E-46
MS.gene057034	MS.gene40087	0.816583	5.13E-52	-1.69E-46
MS.gene057919	MS.gene40087	0.804285	2.32E-49	-1.69E-46
MS.gene058289	MS.gene40087	0.834259	3.32E-56	-1.69E-46
MS.gene058638	MS.gene40087	0.806284	8.86E-50	-1.69E-46
MS.gene058639	MS.gene40087	0.806104	9.67E-50	-1.69E-46
MS.gene058649	MS.gene40087	0.824384	8.31E-54	-1.69E-46
MS.gene058758	MS.gene40087	0.816366	5.74E-52	-1.69E-46
MS.gene059774	MS.gene40087	0.805836	1.10E-49	-1.69E-46
MS.gene059965	MS.gene40087	0.848875	4.64E-60	-1.69E-46
MS.gene060435	MS.gene40087	0.801054	1.08E-48	-1.69E-46
MS.gene060697	MS.gene40087	0.805398	1.36E-49	-1.69E-46
MS.gene060932	MS.gene40087	0.827981	1.16E-54	-1.69E-46
MS.gene060933	MS.gene40087	0.807954	3.93E-50	-1.69E-46
MS.gene061100	MS.gene40087	0.806866	6.68E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene064542	MS.gene40087	PPI
MS.gene019660	MS.gene40087	PPI
MS.gene90825	MS.gene40087	PPI
MS.gene029217	MS.gene40087	PPI
MS.gene40087	MS.gene73119	PPI
MS.gene97346	MS.gene40087	PPI
MS.gene93135	MS.gene40087	PPI
MS.gene056804	MS.gene40087	PPI
MS.gene40087	MS.gene029093	PPI
MS.gene029219	MS.gene40087	PPI
MS.gene035805	MS.gene40087	PPI
MS.gene23912	MS.gene40087	PPI
MS.gene037356	MS.gene40087	PPI
MS.gene84252	MS.gene40087	PPI
MS.gene066439	MS.gene40087	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40087.t1	MTR_4g092900	96.998	533	16	0	1	533	1	533	0.0	1055
MS.gene40087.t1	MTR_4g092900	96.887	514	16	0	1	514	1	514	0.0	1017
MS.gene40087.t1	MTR_4g092880	82.657	542	53	7	1	533	1	510	0.0	820
MS.gene40087.t1	MTR_4g092880	83.422	374	46	5	1	366	1	366	0.0	617
MS.gene40087.t1	MTR_2g091130	45.763	295	132	6	131	425	283	549	5.02e-69	234
MS.gene40087.t1	MTR_2g091135	43.878	294	119	8	141	425	304	560	5.18e-64	220
MS.gene40087.t1	MTR_2g091135	43.878	294	119	8	141	425	304	560	7.17e-64	220
MS.gene40087.t1	MTR_4g079650	40.278	288	101	9	145	425	656	879	4.72e-50	185
MS.gene40087.t1	MTR_4g079650	40.278	288	101	9	145	425	656	879	6.03e-50	185
MS.gene40087.t1	MTR_4g079650	42.041	245	98	8	145	382	656	863	6.16e-46	172
MS.gene40087.t1	MTR_3g075040	28.041	485	226	15	51	487	79	488	3.53e-42	160
MS.gene40087.t1	MTR_3g075040	28.041	485	226	15	51	487	79	488	1.36e-41	159
MS.gene40087.t1	MTR_2g018270	40.571	175	91	5	95	266	29	193	1.29e-26	113
MS.gene40087.t1	MTR_6g033375	26.667	375	213	10	97	460	13	336	6.32e-26	108
MS.gene40087.t1	MTR_8g070660	38.028	142	78	6	109	245	56	192	8.10e-21	92.8
MS.gene40087.t1	MTR_8g070660	36.184	152	88	5	98	245	46	192	1.49e-20	94.0
MS.gene40087.t1	MTR_5g069490	25.581	258	133	7	159	413	111	312	9.92e-16	79.0
MS.gene40087.t1	MTR_2g437920	44.444	81	42	2	167	244	149	229	3.59e-14	74.7
MS.gene40087.t1	MTR_2g033390	88.571	35	4	0	463	497	410	444	3.94e-12	68.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40087.t1	AT1G73150	38.287	397	208	9	37	429	23	386	3.65e-63	213
MS.gene40087.t1	AT1G17790	37.995	379	194	10	55	420	49	399	2.46e-59	204
MS.gene40087.t1	AT1G73150	34.461	473	246	11	37	504	23	436	2.01e-55	193
MS.gene40087.t1	AT1G06230	42.647	272	133	6	144	414	421	670	6.74e-54	195
MS.gene40087.t1	AT1G06230	42.647	272	133	6	144	414	421	670	6.74e-54	195
MS.gene40087.t1	AT1G06230	42.647	272	133	6	144	414	421	670	6.74e-54	195
MS.gene40087.t1	AT1G06230	42.647	272	133	6	144	414	421	670	6.74e-54	195
MS.gene40087.t1	AT5G65630	58.940	151	54	1	337	487	390	532	2.18e-47	174
MS.gene40087.t1	AT5G10550	54.667	150	61	2	337	486	385	527	1.27e-41	158
MS.gene40087.t1	AT5G14270	30.543	442	218	14	77	503	64	431	3.52e-40	155
MS.gene40087.t1	AT5G14270	30.543	442	218	14	77	503	64	431	3.53e-40	155
MS.gene40087.t1	AT5G14270	30.543	442	218	14	77	503	64	431	3.53e-40	155
MS.gene40087.t1	AT5G63320	29.646	452	220	17	64	487	60	441	2.20e-39	150
MS.gene40087.t1	AT5G63320	29.646	452	220	17	64	487	60	441	2.20e-39	150
MS.gene40087.t1	AT5G63320	29.646	452	220	17	64	487	60	441	7.57e-38	149
MS.gene40087.t1	AT3G27260	31.234	381	179	14	104	482	142	441	1.24e-33	135
MS.gene40087.t1	AT3G27260	31.070	383	177	15	104	482	142	441	1.51e-33	136
MS.gene40087.t1	AT3G27260	31.234	381	179	14	104	482	142	441	2.10e-33	135
MS.gene40087.t1	AT3G27260	31.070	383	177	15	104	482	93	392	2.17e-33	135
MS.gene40087.t1	AT3G01770	30.714	420	203	14	82	487	60	405	4.19e-33	132
MS.gene40087.t1	AT3G01770	31.892	370	177	11	145	510	130	428	1.93e-32	132
MS.gene40087.t1	AT5G46550	40.741	135	67	2	148	282	73	194	3.80e-24	106
MS.gene40087.t1	AT3G52280	26.642	274	150	6	143	411	93	320	9.23e-24	103
MS.gene40087.t1	AT3G52280	26.642	274	150	6	143	411	110	337	1.38e-23	103
MS.gene40087.t1	AT2G34900	26.852	324	184	7	143	460	109	385	6.50e-23	101
MS.gene40087.t1	AT2G34900	27.575	301	170	5	166	460	17	275	2.01e-22	97.8
MS.gene40087.t1	AT1G58025	36.036	111	61	3	134	243	209	310	4.49e-11	65.9
MS.gene40087.t1	AT1G58025	36.036	111	61	3	134	243	209	310	4.49e-11	65.9
MS.gene40087.t1	AT1G58025	36.036	111	61	3	134	243	209	310	4.66e-11	65.9
MS.gene40087.t1	AT1G58025	36.036	111	61	3	134	243	209	310	4.66e-11	65.9
MS.gene40087.t1	AT1G58025	36.036	111	61	3	134	243	209	310	4.66e-11	65.9
MS.gene40087.t1	AT1G58025	36.036	111	61	3	134	243	209	310	5.28e-11	65.5

Find 155 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACATTCATTTCCCTCTTATT+TGG	0.124253	4.3:+21246318	None:intergenic
CTTTGGCTTGGGCTGCTTCA+AGG	0.184318	4.3:+21246350	None:intergenic
TTCAAAGCCGTTGCATTTAC+AGG	0.229221	4.3:+21246840	None:intergenic
CCTCTGTGTTGCAGCCAATT+TGG	0.258953	4.3:+21247320	None:intergenic
ACCCTCTCCCTTTCCCTCTC+TGG	0.292850	4.3:+21246537	None:intergenic
TTGTTACTAAATTTAGGGTT+AGG	0.292960	4.3:+21247263	None:intergenic
TCCAGTGGCTCCAGCAGTTC+TGG	0.296431	4.3:-21245746	MS.gene40087:CDS
TCAGGGAGCAAGAAGGTTTC+CGG	0.305508	4.3:-21247008	MS.gene40087:CDS
GAGTCTTAAGCTGACGAATT+TGG	0.320746	4.3:+21247093	None:intergenic
GGATCCCTGGCCTTTGGCTT+GGG	0.322222	4.3:+21246339	None:intergenic
TTCCCTCTTATTTGGATCCC+TGG	0.326630	4.3:+21246326	None:intergenic
TGGATCCCTGGCCTTTGGCT+TGG	0.328127	4.3:+21246338	None:intergenic
TTTGGCTTGGGCTGCTTCAA+GGG	0.331789	4.3:+21246351	None:intergenic
GCATCAACCTTCTCCCTATC+AGG	0.335506	4.3:+21245923	None:intergenic
TCGGATTCAGGGAGTTCTTC+AGG	0.341320	4.3:-21245617	MS.gene40087:CDS
TTATTTGGATCCCTGGCCTT+TGG	0.344705	4.3:+21246333	None:intergenic
ACCCCATTGCCTTTGTTTGA+AGG	0.345351	4.3:+21246057	None:intergenic
GGCTGCTTCAAGGGCTTCAC+TGG	0.348486	4.3:+21246360	None:intergenic
ACCACCGTTGTGGTTTAGCC+TGG	0.356873	4.3:+21247046	None:intergenic
ACCGGTGGAGGGATTGGAAT+TGG	0.357465	4.3:+21246498	None:intergenic
GATTGAGAAAGATAAAGATA+TGG	0.363334	4.3:-21245798	MS.gene40087:CDS
CTGTTGACACGGAGACTCTC+TGG	0.367522	4.3:-21246161	MS.gene40087:CDS
CAGGCTCAGGCAGTGGCTCT+TGG	0.369449	4.3:+21246466	None:intergenic
AATTAAGCATCCTATGGATC+TGG	0.371201	4.3:-21246796	MS.gene40087:CDS
CGAACTGGTGACTGAGCTAA+TGG	0.371584	4.3:+21246408	None:intergenic
TCACTGGAAGTGCTCGAGTT+GGG	0.373259	4.3:+21246376	None:intergenic
ACAACCGTAACGAACCAAAT+TGG	0.373779	4.3:-21247334	MS.gene40087:CDS
GATTAAGCGGCAGGCGCTAA+TGG	0.380098	4.3:-21246094	MS.gene40087:CDS
AAGAAATCAAAGTCAGAGAT+TGG	0.382024	4.3:-21246951	MS.gene40087:CDS
TCATCAAATTTCTCGTGTAT+TGG	0.386676	4.3:+21246609	None:intergenic
CTTGCTCCCTGATTTCTTAG+TGG	0.395685	4.3:+21247019	None:intergenic
ACAGGTGGGAAGTTGTTTGC+TGG	0.397542	4.3:+21245824	None:intergenic
TTGCTCCCTGATTTCTTAGT+GGG	0.400065	4.3:+21247020	None:intergenic
GGTGGTCCCACTAAGAAATC+AGG	0.406385	4.3:-21247026	MS.gene40087:CDS
TTTGCTACCGGTGGAGGGAT+TGG	0.415792	4.3:+21246492	None:intergenic
CTTAAGACTCGAATTGAATC+AGG	0.417831	4.3:-21247080	MS.gene40087:CDS
TGCCTTCAAACAAAGGCAAT+GGG	0.422340	4.3:-21246059	MS.gene40087:CDS
AGGAAAAGGTCGTTTGTTGC+CGG	0.425299	4.3:+21246989	None:intergenic
ACTACTTGTTCCATCTTCTC+TGG	0.427581	4.3:+21246252	None:intergenic
AAGGAAGAGAAATGGGCATT+TGG	0.428825	4.3:-21246220	MS.gene40087:CDS
TTCTGCTTCTTCCCCTCAGA+AGG	0.429158	4.3:+21245893	None:intergenic
CTAAATTTAGGGTTAGGGTT+AGG	0.433996	4.3:+21247269	None:intergenic
AGAAATCAAAGTCAGAGATT+GGG	0.434900	4.3:-21246950	MS.gene40087:CDS
GGATGAGATTGAGCTTGATA+TGG	0.436408	4.3:-21246187	MS.gene40087:CDS
ATTTACAGGAATTGCCTGAT+AGG	0.443388	4.3:-21245937	MS.gene40087:intron
TCGATCGATTGGTTACCAAT+TGG	0.445939	4.3:-21246134	MS.gene40087:CDS
TGTTACTAAATTTAGGGTTA+GGG	0.446081	4.3:+21247264	None:intergenic
AGGCGGTGGAGCTGAATTCA+TGG	0.450284	4.3:-21247300	MS.gene40087:CDS
TGATATAATTAAGCATCCTA+TGG	0.454884	4.3:-21246802	MS.gene40087:CDS
AATTCCTTCACCGCAGGAAA+AGG	0.459833	4.3:+21246975	None:intergenic
AGATCATAAGGAAGAGAAAT+GGG	0.462775	4.3:-21246227	MS.gene40087:CDS
AACAAGTAGTACAGATCATA+AGG	0.463491	4.3:-21246239	MS.gene40087:CDS
ATGGGCATTTGGAGCAAGAT+GGG	0.463782	4.3:-21246209	MS.gene40087:CDS
GCAGCCAATTTGGTTCGTTA+CGG	0.464960	4.3:+21247330	None:intergenic
GATAAAGATATGGGTGCTAC+TGG	0.470996	4.3:-21245788	MS.gene40087:CDS
GCTTCTTCCCCTCAGAAGGA+GGG	0.472060	4.3:+21245897	None:intergenic
CAGATCATAAGGAAGAGAAA+TGG	0.474728	4.3:-21246228	MS.gene40087:CDS
TGCTTCTTCCCCTCAGAAGG+AGG	0.477582	4.3:+21245896	None:intergenic
AGCAGCCCAAGCCAAAGGCC+AGG	0.478818	4.3:-21246344	MS.gene40087:CDS
ACCAGAACTGCTGGAGCCAC+TGG	0.480494	4.3:+21245745	None:intergenic
GTGTTAACATCATGGCCCTT+GGG	0.480899	4.3:+21246675	None:intergenic
AGGCCAGGGATCCAAATAAG+AGG	0.482051	4.3:-21246329	MS.gene40087:CDS
GGGGATTCGAAGTGCTTGGC+TGG	0.485173	4.3:+21246430	None:intergenic
TTATCTTTCTCAATCTCCAC+AGG	0.486073	4.3:+21245806	None:intergenic
AGAGATTGGGGATGCGATGA+AGG	0.491863	4.3:-21246937	MS.gene40087:CDS
GTACTAGCAGGCTCAGGCAG+TGG	0.495175	4.3:+21246459	None:intergenic
GTTGGGGAGGGTATGCGAAC+TGG	0.496924	4.3:+21246393	None:intergenic
CACTGGAAGTGCTCGAGTTG+GGG	0.498490	4.3:+21246377	None:intergenic
GCAGCCCAAGCCAAAGGCCA+GGG	0.500330	4.3:-21246343	MS.gene40087:CDS
ATTGAGAAAGATAAAGATAT+GGG	0.501674	4.3:-21245797	MS.gene40087:CDS
AGTGTTAACATCATGGCCCT+TGG	0.504161	4.3:+21246674	None:intergenic
TTCACTGGAAGTGCTCGAGT+TGG	0.505662	4.3:+21246375	None:intergenic
GTTGGATCGTCGATTGATTG+AGG	0.506931	4.3:+21247208	None:intergenic
TTCTTCAATTCCTTCACCGC+AGG	0.508468	4.3:+21246969	None:intergenic
TTTCCCTCTCTGGTTCAACA+TGG	0.510397	4.3:+21246547	None:intergenic
ATGTTGAACCAGAGAGGGAA+AGG	0.513945	4.3:-21246545	MS.gene40087:CDS
AACCAGAGAGGGAAAGGGAG+AGG	0.515203	4.3:-21246539	MS.gene40087:CDS
CTTCTTCCCCTCAGAAGGAG+GGG	0.516580	4.3:+21245898	None:intergenic
GGTGAGCAAGATTAAGCGGC+AGG	0.516928	4.3:-21246103	MS.gene40087:CDS
TTTACAGGAATTGCCTGATA+GGG	0.522059	4.3:-21245936	MS.gene40087:intron
AATGGGCATTTGGAGCAAGA+TGG	0.522107	4.3:-21246210	MS.gene40087:CDS
ATTGAGGAAGCGTCATCTGA+TGG	0.524535	4.3:+21247224	None:intergenic
TGGCTGCAACACAGAGGCGG+TGG	0.525481	4.3:-21247314	MS.gene40087:CDS
GTGCCTTCAAACAAAGGCAA+TGG	0.528594	4.3:-21246060	MS.gene40087:CDS
GGAATCACTACCAGAACTGC+TGG	0.529979	4.3:+21245736	None:intergenic
CCACCGTTGTGGTTTAGCCT+GGG	0.530502	4.3:+21247047	None:intergenic
GGAGCCACTGGAACTACTAC+TGG	0.536891	4.3:+21245757	None:intergenic
TTGAGGAAGCGTCATCTGAT+GGG	0.538515	4.3:+21247225	None:intergenic
ATTAAGCATCCTATGGATCT+GGG	0.539150	4.3:-21246795	MS.gene40087:CDS
GAACTGGTGACTGAGCTAAT+GGG	0.540318	4.3:+21246409	None:intergenic
TTAGAAGTACTAGCAGGCTC+AGG	0.542104	4.3:+21246453	None:intergenic
GTATTGTGAATGATTATCGT+TGG	0.542418	4.3:+21247190	None:intergenic
TCTTTCTCAATCTCCACAGG+TGG	0.544866	4.3:+21245809	None:intergenic
TCAGGAAGTGACTCTGAAGC+GGG	0.549573	4.3:-21245599	MS.gene40087:CDS
TGATGCAACCCCTCCTTCTG+AGG	0.549763	4.3:-21245906	MS.gene40087:CDS
GAAATCAAAGTCAGAGATTG+GGG	0.550032	4.3:-21246949	MS.gene40087:CDS
AATAATGTGCCTTCAAACAA+AGG	0.557916	4.3:-21246066	MS.gene40087:CDS
ACTTGTTCCATCTTCTCTGG+TGG	0.558187	4.3:+21246255	None:intergenic
AGTGACTCTGAAGCGGGCAA+TGG	0.561285	4.3:-21245593	MS.gene40087:CDS
GGCTGGTTAGAAGTACTAGC+AGG	0.564980	4.3:+21246447	None:intergenic
TGTTGACACGGAGACTCTCT+GGG	0.565659	4.3:-21246160	MS.gene40087:CDS
GATGCAACCCCTCCTTCTGA+GGG	0.565783	4.3:-21245905	MS.gene40087:CDS
TGGAAGTGCTCGAGTTGGGG+AGG	0.565929	4.3:+21246380	None:intergenic
TGTTGAACCAGAGAGGGAAA+GGG	0.566133	4.3:-21246544	MS.gene40087:CDS
GGCGGTGGAGCTGAATTCAT+GGG	0.566531	4.3:-21247299	MS.gene40087:CDS
CTTGAAGCAGCCCAAGCCAA+AGG	0.568683	4.3:-21246349	MS.gene40087:CDS
ACCAGAGAGGGAAAGGGAGA+GGG	0.569026	4.3:-21246538	MS.gene40087:CDS
TCCAATTCCAATCCCTCCAC+CGG	0.570278	4.3:-21246499	MS.gene40087:CDS
TCTCTGGGAACTCGATCGAT+TGG	0.573945	4.3:-21246145	MS.gene40087:CDS
TTCAGGAAGTGACTCTGAAG+CGG	0.578365	4.3:-21245600	MS.gene40087:CDS
TCGCCGCAGTGTTAACATCA+TGG	0.579958	4.3:+21246667	None:intergenic
ATGAATTACAGGCTAGCTCG+TGG	0.582142	4.3:-21246572	MS.gene40087:CDS
CATCGAATCGTACACAAAGA+CGG	0.584140	4.3:-21247156	MS.gene40087:CDS
TAGTGGGACCACCACCGTTG+TGG	0.584586	4.3:+21247036	None:intergenic
AACTGGTGACTGAGCTAATG+GGG	0.588096	4.3:+21246410	None:intergenic
TGCATTGACATACAATCCCA+AGG	0.588241	4.3:-21246691	MS.gene40087:CDS
AGCAAACAACTTCCCACCTG+TGG	0.593833	4.3:-21245822	MS.gene40087:CDS
AGGCTAAACCACAACGGTGG+TGG	0.594359	4.3:-21247044	MS.gene40087:CDS
TTGACAGTACCCAGATCCAT+AGG	0.596367	4.3:+21246786	None:intergenic
GATATGGGTGCTACTGGTGG+CGG	0.601785	4.3:-21245782	MS.gene40087:CDS
TAACACTCCTGTAAATGCAA+CGG	0.607221	4.3:-21246847	MS.gene40087:CDS
GGAAGTGCTCGAGTTGGGGA+GGG	0.608519	4.3:+21246381	None:intergenic
GGCCAGGGATCCAAATAAGA+GGG	0.609397	4.3:-21246328	MS.gene40087:CDS
GGTAGTGATTCCTCGTCTAG+TGG	0.609600	4.3:-21245725	MS.gene40087:intron
GAACCATGTTGAACCAGAGA+GGG	0.610145	4.3:-21246550	MS.gene40087:CDS
CTTTCTCAATCTCCACAGGT+GGG	0.611329	4.3:+21245810	None:intergenic
GTGGTCCCACTAAGAAATCA+GGG	0.612863	4.3:-21247025	MS.gene40087:CDS
TAAGAGGGAAATGAATGTTG+AGG	0.616464	4.3:-21246313	MS.gene40087:CDS
TGATATGGAGGCTGTTGACA+CGG	0.617215	4.3:-21246172	MS.gene40087:CDS
GGAATTGCCTGATAGGGAGA+AGG	0.617711	4.3:-21245930	MS.gene40087:CDS
ACATCAGCAGCAAAATCCGC+CGG	0.619449	4.3:+21246732	None:intergenic
TGAGGAAGCGTCATCTGATG+GGG	0.626247	4.3:+21247226	None:intergenic
AGATGGTGAGCAAGATTAAG+CGG	0.630774	4.3:-21246107	MS.gene40087:CDS
TAAGAAATCAGGGAGCAAGA+AGG	0.642181	4.3:-21247015	MS.gene40087:CDS
AAAGATATGGGTGCTACTGG+TGG	0.649739	4.3:-21245785	MS.gene40087:CDS
TGGGCATTTGGAGCAAGATG+GGG	0.655326	4.3:-21246208	MS.gene40087:CDS
TGAGATTGAGCTTGATATGG+AGG	0.664012	4.3:-21246184	MS.gene40087:CDS
GGAACCATGTTGAACCAGAG+AGG	0.667661	4.3:-21246551	MS.gene40087:CDS
GGGGATGCGATGAAGGCTTG+TGG	0.668675	4.3:-21246930	MS.gene40087:CDS
GAAGATAGATACTGTTGACG+AGG	0.670346	4.3:-21245870	MS.gene40087:CDS
GGTGCCAGTAGTAGTTCCAG+TGG	0.670411	4.3:-21245761	MS.gene40087:CDS
TAAGAATGCTTACTCTACGC+CGG	0.674848	4.3:-21246751	MS.gene40087:CDS
GCATTGACATACAATCCCAA+GGG	0.679665	4.3:-21246690	MS.gene40087:CDS
AATTGGCTGCAACACAGAGG+CGG	0.689298	4.3:-21247317	MS.gene40087:CDS
ATGCAACCCCTCCTTCTGAG+GGG	0.696569	4.3:-21245904	MS.gene40087:CDS
AATCAGGTGAGTTTAAACCC+AGG	0.697049	4.3:-21247064	MS.gene40087:CDS
GCCTTCAAACAAAGGCAATG+GGG	0.698333	4.3:-21246058	MS.gene40087:intron
ACTGGTGACTGAGCTAATGG+GGG	0.699815	4.3:+21246411	None:intergenic
AGAGGTTATACCACTAGACG+AGG	0.704995	4.3:+21245715	None:intergenic
GAATGCTTACTCTACGCCGG+CGG	0.711544	4.3:-21246748	MS.gene40087:CDS
CCCAGGCTAAACCACAACGG+TGG	0.730798	4.3:-21247047	MS.gene40087:CDS
CCAAATTGGCTGCAACACAG+AGG	0.734243	4.3:-21247320	MS.gene40087:CDS
GTTGACGAGGATGTTGACAT+CGG	0.736538	4.3:-21245857	MS.gene40087:CDS
AAACCCAGGCTAAACCACAA+CGG	0.749218	4.3:-21247050	MS.gene40087:CDS
GGGCCATGATGTTAACACTG+CGG	0.770423	4.3:-21246670	MS.gene40087:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCTTTTTGTTACTAAATTTA+GGG	+	chr4.3:21245681-21245700	None:intergenic	15.0%
!!	ATTGAGAAAGATAAAGATAT+GGG	-	chr4.3:21247139-21247158	MS.gene40087:CDS	20.0%
!!!	CTCTTTTTGTTACTAAATTT+AGG	+	chr4.3:21245682-21245701	None:intergenic	20.0%
!!!	TTGTATTCTGAAATTATCAT+GGG	-	chr4.3:21247265-21247284	MS.gene40087:CDS	20.0%
!!!	TTTGTATTCTGAAATTATCA+TGG	-	chr4.3:21247264-21247283	MS.gene40087:CDS	20.0%
!	ATCACTCAACAGAATAAAAA+TGG	+	chr4.3:21246919-21246938	None:intergenic	25.0%
!	CAGAATAAAAATGGACAATA+AGG	+	chr4.3:21246910-21246929	None:intergenic	25.0%
!	GATTGAGAAAGATAAAGATA+TGG	-	chr4.3:21247138-21247157	MS.gene40087:CDS	25.0%
!	TGATATAATTAAGCATCCTA+TGG	-	chr4.3:21246134-21246153	MS.gene40087:CDS	25.0%
!!	TGTTACTAAATTTAGGGTTA+GGG	+	chr4.3:21245675-21245694	None:intergenic	25.0%
!!	TTGTTACTAAATTTAGGGTT+AGG	+	chr4.3:21245676-21245695	None:intergenic	25.0%
!!!	AAGTTTTGATGATGAATTAC+AGG	-	chr4.3:21246353-21246372	MS.gene40087:CDS	25.0%
	AACAAGTAGTACAGATCATA+AGG	-	chr4.3:21246697-21246716	MS.gene40087:CDS	30.0%
	AAGAAATCAAAGTCAGAGAT+TGG	-	chr4.3:21245985-21246004	MS.gene40087:intron	30.0%
	AAGCTCATGAAAAACAAAAG+TGG	-	chr4.3:21246042-21246061	MS.gene40087:intron	30.0%
	AATAATGTGCCTTCAAACAA+AGG	-	chr4.3:21246870-21246889	MS.gene40087:CDS	30.0%
	ACAAACCATAAAACATTCAG+TGG	+	chr4.3:21247249-21247268	None:intergenic	30.0%
	ACATTCATTTCCCTCTTATT+TGG	+	chr4.3:21246621-21246640	None:intergenic	30.0%
	AGAAATCAAAGTCAGAGATT+GGG	-	chr4.3:21245986-21246005	MS.gene40087:intron	30.0%
	AGATCATAAGGAAGAGAAAT+GGG	-	chr4.3:21246709-21246728	MS.gene40087:CDS	30.0%
	ATAAAACATTCAGTGGAAAG+AGG	+	chr4.3:21247242-21247261	None:intergenic	30.0%
	GTATTGTGAATGATTATCGT+TGG	+	chr4.3:21245749-21245768	None:intergenic	30.0%
	GTTGAGGAAAAACATAAGTT+GGG	-	chr4.3:21246639-21246658	MS.gene40087:CDS	30.0%
	TCATCAAATTTCTCGTGTAT+TGG	+	chr4.3:21246330-21246349	None:intergenic	30.0%
	TCATGAAAAACAAAAGTGGT+TGG	-	chr4.3:21246046-21246065	MS.gene40087:intron	30.0%
	TGTTGAGGAAAAACATAAGT+TGG	-	chr4.3:21246638-21246657	MS.gene40087:CDS	30.0%
!	AAAAACATAAGTTGGGACTA+GGG	-	chr4.3:21246646-21246665	MS.gene40087:CDS	30.0%
!	ATTTTCTTGTGTGCATTTAC+AGG	-	chr4.3:21246985-21247004	MS.gene40087:CDS	30.0%
!	TCTTTCCACTGAATGTTTTA+TGG	-	chr4.3:21247241-21247260	MS.gene40087:CDS	30.0%
!	TTTCGATACTTTTGCAGATT+CGG	-	chr4.3:21247300-21247319	MS.gene40087:CDS	30.0%
	AATTAAGCATCCTATGGATC+TGG	-	chr4.3:21246140-21246159	MS.gene40087:CDS	35.0%
	ATTAAGCATCCTATGGATCT+GGG	-	chr4.3:21246141-21246160	MS.gene40087:CDS	35.0%
	ATTTACAGGAATTGCCTGAT+AGG	-	chr4.3:21246999-21247018	MS.gene40087:CDS	35.0%
	CAGATCATAAGGAAGAGAAA+TGG	-	chr4.3:21246708-21246727	MS.gene40087:CDS	35.0%
	CTTAAGACTCGAATTGAATC+AGG	-	chr4.3:21245856-21245875	MS.gene40087:CDS	35.0%
	GAAATCAAAGTCAGAGATTG+GGG	-	chr4.3:21245987-21246006	MS.gene40087:intron	35.0%
	GGTTACCAATTGGAAAAAGA+TGG	-	chr4.3:21246812-21246831	MS.gene40087:CDS	35.0%
	TAACACTCCTGTAAATGCAA+CGG	-	chr4.3:21246089-21246108	MS.gene40087:CDS	35.0%
	TAAGAGGGAAATGAATGTTG+AGG	-	chr4.3:21246623-21246642	MS.gene40087:CDS	35.0%
	TTATCTTTCTCAATCTCCAC+AGG	+	chr4.3:21247133-21247152	None:intergenic	35.0%
	TTTACAGGAATTGCCTGATA+GGG	-	chr4.3:21247000-21247019	MS.gene40087:CDS	35.0%
!	CTAAATTTAGGGTTAGGGTT+AGG	+	chr4.3:21245670-21245689	None:intergenic	35.0%
!	GAAAAACATAAGTTGGGACT+AGG	-	chr4.3:21246645-21246664	MS.gene40087:CDS	35.0%
!	GTGGTTGGATTTTTAACACT+TGG	-	chr4.3:21246061-21246080	MS.gene40087:CDS	35.0%
!!	CTGCAAAAGTATCGAAATTG+AGG	+	chr4.3:21247297-21247316	None:intergenic	35.0%
	AAGGAAGAGAAATGGGCATT+TGG	-	chr4.3:21246716-21246735	MS.gene40087:CDS	40.0%
	AATCAGGTGAGTTTAAACCC+AGG	-	chr4.3:21245872-21245891	MS.gene40087:CDS	40.0%
	ACAACCGTAACGAACCAAAT+TGG	-	chr4.3:21245602-21245621	MS.gene40087:CDS	40.0%
	ACTACTTGTTCCATCTTCTC+TGG	+	chr4.3:21246687-21246706	None:intergenic	40.0%
	AGATGGTGAGCAAGATTAAG+CGG	-	chr4.3:21246829-21246848	MS.gene40087:CDS	40.0%
	CATCGAATCGTACACAAAGA+CGG	-	chr4.3:21245780-21245799	MS.gene40087:CDS	40.0%
	GAAGATAGATACTGTTGACG+AGG	-	chr4.3:21247066-21247085	MS.gene40087:CDS	40.0%
	GAGTCTTAAGCTGACGAATT+TGG	+	chr4.3:21245846-21245865	None:intergenic	40.0%
	GCAGCTTCTTGAAAAATTCG+AGG	-	chr4.3:21246293-21246312	MS.gene40087:CDS	40.0%
	GCTGTCTAATGTAGCATTGT+TGG	-	chr4.3:21246942-21246961	MS.gene40087:CDS	40.0%
	GGATGAGATTGAGCTTGATA+TGG	-	chr4.3:21246749-21246768	MS.gene40087:CDS	40.0%
	TAAGAAATCAGGGAGCAAGA+AGG	-	chr4.3:21245921-21245940	MS.gene40087:CDS	40.0%
	TAAGAATGCTTACTCTACGC+CGG	-	chr4.3:21246185-21246204	MS.gene40087:CDS	40.0%
	TGAGATTGAGCTTGATATGG+AGG	-	chr4.3:21246752-21246771	MS.gene40087:CDS	40.0%
	TGCCTTCAAACAAAGGCAAT+GGG	-	chr4.3:21246877-21246896	MS.gene40087:CDS	40.0%
	TTCAAAGCCGTTGCATTTAC+AGG	+	chr4.3:21246099-21246118	None:intergenic	40.0%
	TTGCTCCCTGATTTCTTAGT+GGG	+	chr4.3:21245919-21245938	None:intergenic	40.0%
!	ACTTTTGCAGATTCGGATTC+AGG	-	chr4.3:21247307-21247326	MS.gene40087:CDS	40.0%
!	CTTTTGCAGATTCGGATTCA+GGG	-	chr4.3:21247308-21247327	MS.gene40087:CDS	40.0%
!	GATAAAGATATGGGTGCTAC+TGG	-	chr4.3:21247148-21247167	MS.gene40087:CDS	40.0%
!	GCATTGACATACAATCCCAA+GGG	-	chr4.3:21246246-21246265	MS.gene40087:CDS	40.0%
!	GCTCACCATCTTTTTCCAAT+TGG	+	chr4.3:21246820-21246839	None:intergenic	40.0%
!	TCGATCGATTGGTTACCAAT+TGG	-	chr4.3:21246802-21246821	MS.gene40087:CDS	40.0%
!	TGCATTGACATACAATCCCA+AGG	-	chr4.3:21246245-21246264	MS.gene40087:CDS	40.0%
	AAACCCAGGCTAAACCACAA+CGG	-	chr4.3:21245886-21245905	MS.gene40087:CDS	45.0%
	AACTGGTGACTGAGCTAATG+GGG	+	chr4.3:21246529-21246548	None:intergenic	45.0%
	AATTCCTTCACCGCAGGAAA+AGG	+	chr4.3:21245964-21245983	None:intergenic	45.0%
	ACTTGTTCCATCTTCTCTGG+TGG	+	chr4.3:21246684-21246703	None:intergenic	45.0%
	AGAGGTTATACCACTAGACG+AGG	+	chr4.3:21247224-21247243	None:intergenic	45.0%
	AGTGTTAACATCATGGCCCT+TGG	+	chr4.3:21246265-21246284	None:intergenic	45.0%
	ATGTTGAACCAGAGAGGGAA+AGG	-	chr4.3:21246391-21246410	MS.gene40087:CDS	45.0%
	CTTGCTCCCTGATTTCTTAG+TGG	+	chr4.3:21245920-21245939	None:intergenic	45.0%
	CTTTCTCAATCTCCACAGGT+GGG	+	chr4.3:21247129-21247148	None:intergenic	45.0%
	GAACCATGTTGAACCAGAGA+GGG	-	chr4.3:21246386-21246405	MS.gene40087:CDS	45.0%
	GAACTGGTGACTGAGCTAAT+GGG	+	chr4.3:21246530-21246549	None:intergenic	45.0%
	GCCTTCAAACAAAGGCAATG+GGG	-	chr4.3:21246878-21246897	MS.gene40087:CDS	45.0%
	GTGCCTTCAAACAAAGGCAA+TGG	-	chr4.3:21246876-21246895	MS.gene40087:CDS	45.0%
	GTGGTCCCACTAAGAAATCA+GGG	-	chr4.3:21245911-21245930	MS.gene40087:CDS	45.0%
	GTGTTAACATCATGGCCCTT+GGG	+	chr4.3:21246264-21246283	None:intergenic	45.0%
	GTTGACGAGGATGTTGACAT+CGG	-	chr4.3:21247079-21247098	MS.gene40087:CDS	45.0%
	TCTTTCTCAATCTCCACAGG+TGG	+	chr4.3:21247130-21247149	None:intergenic	45.0%
	TGATATGGAGGCTGTTGACA+CGG	-	chr4.3:21246764-21246783	MS.gene40087:CDS	45.0%
	TGTTGAACCAGAGAGGGAAA+GGG	-	chr4.3:21246392-21246411	MS.gene40087:CDS	45.0%
	TTCTTCAATTCCTTCACCGC+AGG	+	chr4.3:21245970-21245989	None:intergenic	45.0%
	TTGACAGTACCCAGATCCAT+AGG	+	chr4.3:21246153-21246172	None:intergenic	45.0%
!	AATGGGCATTTGGAGCAAGA+TGG	-	chr4.3:21246726-21246745	MS.gene40087:CDS	45.0%
!	ACCCCATTGCCTTTGTTTGA+AGG	+	chr4.3:21246882-21246901	None:intergenic	45.0%
!	AGGAAAAGGTCGTTTGTTGC+CGG	+	chr4.3:21245950-21245969	None:intergenic	45.0%
!	ATGAATTACAGGCTAGCTCG+TGG	-	chr4.3:21246364-21246383	MS.gene40087:CDS	45.0%
!	ATGGGCATTTGGAGCAAGAT+GGG	-	chr4.3:21246727-21246746	MS.gene40087:CDS	45.0%
!	CAACAAACGACCTTTTCCTG+CGG	-	chr4.3:21245951-21245970	MS.gene40087:intron	45.0%
!	GATTTTGCCACCAGAGAAGA+TGG	-	chr4.3:21246674-21246693	MS.gene40087:CDS	45.0%
!	GCAGCCAATTTGGTTCGTTA+CGG	+	chr4.3:21245609-21245628	None:intergenic	45.0%
!	GTTGGATCGTCGATTGATTG+AGG	+	chr4.3:21245731-21245750	None:intergenic	45.0%
!	TTATTTGGATCCCTGGCCTT+TGG	+	chr4.3:21246606-21246625	None:intergenic	45.0%
!	TTCAGGAAGTGACTCTGAAG+CGG	-	chr4.3:21247336-21247355	MS.gene40087:CDS	45.0%
!	TTCCCTCTTATTTGGATCCC+TGG	+	chr4.3:21246613-21246632	None:intergenic	45.0%
!	TTTCCCTCTCTGGTTCAACA+TGG	+	chr4.3:21246392-21246411	None:intergenic	45.0%
!!	AAAGATATGGGTGCTACTGG+TGG	-	chr4.3:21247151-21247170	MS.gene40087:CDS	45.0%
!!	ATTGAGGAAGCGTCATCTGA+TGG	+	chr4.3:21245715-21245734	None:intergenic	45.0%
!!	TTAGAAGTACTAGCAGGCTC+AGG	+	chr4.3:21246486-21246505	None:intergenic	45.0%
!!	TTGAGGAAGCGTCATCTGAT+GGG	+	chr4.3:21245714-21245733	None:intergenic	45.0%
	AATTGGCTGCAACACAGAGG+CGG	-	chr4.3:21245619-21245638	MS.gene40087:CDS	50.0%
	ACAGGTGGGAAGTTGTTTGC+TGG	+	chr4.3:21247115-21247134	None:intergenic	50.0%
	ACATCAGCAGCAAAATCCGC+CGG	+	chr4.3:21246207-21246226	None:intergenic	50.0%
	ACTGGTGACTGAGCTAATGG+GGG	+	chr4.3:21246528-21246547	None:intergenic	50.0%
	AGAGATTGGGGATGCGATGA+AGG	-	chr4.3:21245999-21246018	MS.gene40087:intron	50.0%
	AGCAAACAACTTCCCACCTG+TGG	-	chr4.3:21247114-21247133	MS.gene40087:CDS	50.0%
	AGGCCAGGGATCCAAATAAG+AGG	-	chr4.3:21246607-21246626	MS.gene40087:CDS	50.0%
	CCAAATTGGCTGCAACACAG+AGG	-	chr4.3:21245616-21245635	MS.gene40087:CDS	50.0%
	CCTCTGTGTTGCAGCCAATT+TGG	+	chr4.3:21245619-21245638	None:intergenic	50.0%
	CGAACTGGTGACTGAGCTAA+TGG	+	chr4.3:21246531-21246550	None:intergenic	50.0%
	GCATCAACCTTCTCCCTATC+AGG	+	chr4.3:21247016-21247035	None:intergenic	50.0%
	GGAACCATGTTGAACCAGAG+AGG	-	chr4.3:21246385-21246404	MS.gene40087:CDS	50.0%
	GGAATCACTACCAGAACTGC+TGG	+	chr4.3:21247203-21247222	None:intergenic	50.0%
	GGAATTGCCTGATAGGGAGA+AGG	-	chr4.3:21247006-21247025	MS.gene40087:CDS	50.0%
	GGCCAGGGATCCAAATAAGA+GGG	-	chr4.3:21246608-21246627	MS.gene40087:CDS	50.0%
	GGGCCATGATGTTAACACTG+CGG	-	chr4.3:21246266-21246285	MS.gene40087:CDS	50.0%
	GGTAGTGATTCCTCGTCTAG+TGG	-	chr4.3:21247211-21247230	MS.gene40087:CDS	50.0%
	GGTGGTCCCACTAAGAAATC+AGG	-	chr4.3:21245910-21245929	MS.gene40087:CDS	50.0%
	TCAGGGAGCAAGAAGGTTTC+CGG	-	chr4.3:21245928-21245947	MS.gene40087:CDS	50.0%
	TCCAATTCCAATCCCTCCAC+CGG	-	chr4.3:21246437-21246456	MS.gene40087:CDS	50.0%
	TCGCCGCAGTGTTAACATCA+TGG	+	chr4.3:21246272-21246291	None:intergenic	50.0%
	TCGGATTCAGGGAGTTCTTC+AGG	-	chr4.3:21247319-21247338	MS.gene40087:CDS	50.0%
	TGTTGACACGGAGACTCTCT+GGG	-	chr4.3:21246776-21246795	MS.gene40087:CDS	50.0%
	TTCTGCTTCTTCCCCTCAGA+AGG	+	chr4.3:21247046-21247065	None:intergenic	50.0%
!	AATGGGGGATTCGAAGTGCT+TGG	+	chr4.3:21246513-21246532	None:intergenic	50.0%
!	TCTCTGGGAACTCGATCGAT+TGG	-	chr4.3:21246791-21246810	MS.gene40087:CDS	50.0%
!	TGGGCATTTGGAGCAAGATG+GGG	-	chr4.3:21246728-21246747	MS.gene40087:CDS	50.0%
!!	GGCTGGTTAGAAGTACTAGC+AGG	+	chr4.3:21246492-21246511	None:intergenic	50.0%
!!	TCACTGGAAGTGCTCGAGTT+GGG	+	chr4.3:21246563-21246582	None:intergenic	50.0%
!!	TCAGGAAGTGACTCTGAAGC+GGG	-	chr4.3:21247337-21247356	MS.gene40087:CDS	50.0%
!!	TGAGGAAGCGTCATCTGATG+GGG	+	chr4.3:21245713-21245732	None:intergenic	50.0%
!!	TTCACTGGAAGTGCTCGAGT+TGG	+	chr4.3:21246564-21246583	None:intergenic	50.0%
!!	TTTGGCTTGGGCTGCTTCAA+GGG	+	chr4.3:21246588-21246607	None:intergenic	50.0%
	AACCAGAGAGGGAAAGGGAG+AGG	-	chr4.3:21246397-21246416	MS.gene40087:CDS	55.0%
	ACCACCGTTGTGGTTTAGCC+TGG	+	chr4.3:21245893-21245912	None:intergenic	55.0%
	ACCAGAGAGGGAAAGGGAGA+GGG	-	chr4.3:21246398-21246417	MS.gene40087:CDS	55.0%
	ACCGGTGGAGGGATTGGAAT+TGG	+	chr4.3:21246441-21246460	None:intergenic	55.0%
	AGGCGGTGGAGCTGAATTCA+TGG	-	chr4.3:21245636-21245655	MS.gene40087:intron	55.0%
	AGGCTAAACCACAACGGTGG+TGG	-	chr4.3:21245892-21245911	MS.gene40087:CDS	55.0%
	ATGCAACCCCTCCTTCTGAG+GGG	-	chr4.3:21247032-21247051	MS.gene40087:CDS	55.0%
	CCACCGTTGTGGTTTAGCCT+GGG	+	chr4.3:21245892-21245911	None:intergenic	55.0%
	CTGTTGACACGGAGACTCTC+TGG	-	chr4.3:21246775-21246794	MS.gene40087:CDS	55.0%
	CTTCTTCCCCTCAGAAGGAG+GGG	+	chr4.3:21247041-21247060	None:intergenic	55.0%
	CTTGAAGCAGCCCAAGCCAA+AGG	-	chr4.3:21246587-21246606	MS.gene40087:CDS	55.0%
	GAATGCTTACTCTACGCCGG+CGG	-	chr4.3:21246188-21246207	MS.gene40087:CDS	55.0%
	GATGCAACCCCTCCTTCTGA+GGG	-	chr4.3:21247031-21247050	MS.gene40087:CDS	55.0%
	GATTAAGCGGCAGGCGCTAA+TGG	-	chr4.3:21246842-21246861	MS.gene40087:CDS	55.0%
	GCTTCTTCCCCTCAGAAGGA+GGG	+	chr4.3:21247042-21247061	None:intergenic	55.0%
	GGAGCCACTGGAACTACTAC+TGG	+	chr4.3:21247182-21247201	None:intergenic	55.0%
	GGCGGTGGAGCTGAATTCAT+GGG	-	chr4.3:21245637-21245656	MS.gene40087:intron	55.0%
	GGTGAGCAAGATTAAGCGGC+AGG	-	chr4.3:21246833-21246852	MS.gene40087:CDS	55.0%
	GGTGCCAGTAGTAGTTCCAG+TGG	-	chr4.3:21247175-21247194	MS.gene40087:CDS	55.0%
	TGATGCAACCCCTCCTTCTG+AGG	-	chr4.3:21247030-21247049	MS.gene40087:CDS	55.0%
	TGCTTCTTCCCCTCAGAAGG+AGG	+	chr4.3:21247043-21247062	None:intergenic	55.0%
	TTTGCTACCGGTGGAGGGAT+TGG	+	chr4.3:21246447-21246466	None:intergenic	55.0%
!	ACGACCTTTTCCTGCGGTGA+AGG	-	chr4.3:21245957-21245976	MS.gene40087:intron	55.0%
!	GGCTCTTGGCGTTTTGCTAC+CGG	+	chr4.3:21246459-21246478	None:intergenic	55.0%
!	TCTTGGCGTTTTGCTACCGG+TGG	+	chr4.3:21246456-21246475	None:intergenic	55.0%
!!	AGTGACTCTGAAGCGGGCAA+TGG	-	chr4.3:21247343-21247362	MS.gene40087:CDS	55.0%
!!	CACTGGAAGTGCTCGAGTTG+GGG	+	chr4.3:21246562-21246581	None:intergenic	55.0%
!!	CTTTGGCTTGGGCTGCTTCA+AGG	+	chr4.3:21246589-21246608	None:intergenic	55.0%
!!	GATATGGGTGCTACTGGTGG+CGG	-	chr4.3:21247154-21247173	MS.gene40087:CDS	55.0%
	ACCAGAACTGCTGGAGCCAC+TGG	+	chr4.3:21247194-21247213	None:intergenic	60.0%
	ACCCTCTCCCTTTCCCTCTC+TGG	+	chr4.3:21246402-21246421	None:intergenic	60.0%
	CCCAGGCTAAACCACAACGG+TGG	-	chr4.3:21245889-21245908	MS.gene40087:CDS	60.0%
	GGCTGCTTCAAGGGCTTCAC+TGG	+	chr4.3:21246579-21246598	None:intergenic	60.0%
	GGGGATGCGATGAAGGCTTG+TGG	-	chr4.3:21246006-21246025	MS.gene40087:intron	60.0%
	GTACTAGCAGGCTCAGGCAG+TGG	+	chr4.3:21246480-21246499	None:intergenic	60.0%
	TAGTGGGACCACCACCGTTG+TGG	+	chr4.3:21245903-21245922	None:intergenic	60.0%
	TCCAGTGGCTCCAGCAGTTC+TGG	-	chr4.3:21247190-21247209	MS.gene40087:CDS	60.0%
!	GGATCCCTGGCCTTTGGCTT+GGG	+	chr4.3:21246600-21246619	None:intergenic	60.0%
!	GGCGTTTTGCTACCGGTGGA+GGG	+	chr4.3:21246452-21246471	None:intergenic	60.0%
!	GGGGATTCGAAGTGCTTGGC+TGG	+	chr4.3:21246509-21246528	None:intergenic	60.0%
!	GTTGGGGAGGGTATGCGAAC+TGG	+	chr4.3:21246546-21246565	None:intergenic	60.0%
!	TGGATCCCTGGCCTTTGGCT+TGG	+	chr4.3:21246601-21246620	None:intergenic	60.0%
!	TGGCGTTTTGCTACCGGTGG+AGG	+	chr4.3:21246453-21246472	None:intergenic	60.0%
!!	GGAAGTGCTCGAGTTGGGGA+GGG	+	chr4.3:21246558-21246577	None:intergenic	60.0%
!!	TGGAAGTGCTCGAGTTGGGG+AGG	+	chr4.3:21246559-21246578	None:intergenic	60.0%
	AGCAGCCCAAGCCAAAGGCC+AGG	-	chr4.3:21246592-21246611	MS.gene40087:CDS	65.0%
	CAGGCTCAGGCAGTGGCTCT+TGG	+	chr4.3:21246473-21246492	None:intergenic	65.0%
	GCAGCCCAAGCCAAAGGCCA+GGG	-	chr4.3:21246593-21246612	MS.gene40087:CDS	65.0%
	TGGCTGCAACACAGAGGCGG+TGG	-	chr4.3:21245622-21245641	MS.gene40087:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	21245583	21247375	21245583	ID=MS.gene40087
chr4.3	mRNA	21245583	21247375	21245583	ID=MS.gene40087.t1;Parent=MS.gene40087
chr4.3	exon	21246059	21247375	21246059	ID=MS.gene40087.t1.exon1;Parent=MS.gene40087.t1
chr4.3	CDS	21246059	21247375	21246059	ID=cds.MS.gene40087.t1;Parent=MS.gene40087.t1
chr4.3	exon	21245726	21245951	21245726	ID=MS.gene40087.t1.exon2;Parent=MS.gene40087.t1
chr4.3	CDS	21245726	21245951	21245726	ID=cds.MS.gene40087.t1;Parent=MS.gene40087.t1
chr4.3	exon	21245583	21245641	21245583	ID=MS.gene40087.t1.exon3;Parent=MS.gene40087.t1
chr4.3	CDS	21245583	21245641	21245583	ID=cds.MS.gene40087.t1;Parent=MS.gene40087.t1

Gene Sequence

>MS.gene40087

ATGGCATCGGCTTTATTAGACAACCGTAACGAACCAAATTGGCTGCAACACAGAGGCGGTGGAGCTGAATTCATGGGTAAAGCACCTAACCCTAACCCTAAATTTAGTAACAAAAAGAGAACTCAATCCCCATCAGATGACGCTTCCTCAATCAATCGACGATCCAACGATAATCATTCACAATACGTTACTTTCAACATCGAATCGTACACAAAGACGGAGCTATACGAGCTCAAAAATCGACTCGTATCAGAACTCGACCAAATTCGTCAGCTTAAGACTCGAATTGAATCAGGTGAGTTTAAACCCAGGCTAAACCACAACGGTGGTGGTCCCACTAAGAAATCAGGGAGCAAGAAGGTTTCCGGCAACAAACGACCTTTTCCTGCGGTGAAGGAATTGAAGAAATCAAAGTCAGAGATTGGGGATGCGATGAAGGCTTGTGGTCAGATTTTGACGAAGCTCATGAAAAACAAAAGTGGTTGGATTTTTAACACTTGGATCTTTAACACTCCTGTAAATGCAACGGCTTTGAATCTTCATGATTACTTTGATATAATTAAGCATCCTATGGATCTGGGTACTGTCAAGTCAAAACTTGCTAAGAATGCTTACTCTACGCCGGCGGATTTTGCTGCTGATGTGAAGTTAACGTTCAACAATGCATTGACATACAATCCCAAGGGCCATGATGTTAACACTGCGGCGAAGCAGCTTCTTGAAAAATTCGAGGAGCTTTATCGACCAATACACGAGAAATTTGATGAAAAAAGTTTTGATGATGAATTACAGGCTAGCTCGTGGAACCATGTTGAACCAGAGAGGGAAAGGGAGAGGGTGAAGAAGAAAGATAATCCAATTCCAATCCCTCCACCGGTAGCAAAACGCCAAGAGCCACTGCCTGAGCCTGCTAGTACTTCTAACCAGCCAAGCACTTCGAATCCCCCATTAGCTCAGTCACCAGTTCGCATACCCTCCCCAACTCGAGCACTTCCAGTGAAGCCCTTGAAGCAGCCCAAGCCAAAGGCCAGGGATCCAAATAAGAGGGAAATGAATGTTGAGGAAAAACATAAGTTGGGACTAGGGTTGCAGATTTTGCCACCAGAGAAGATGGAACAAGTAGTACAGATCATAAGGAAGAGAAATGGGCATTTGGAGCAAGATGGGGATGAGATTGAGCTTGATATGGAGGCTGTTGACACGGAGACTCTCTGGGAACTCGATCGATTGGTTACCAATTGGAAAAAGATGGTGAGCAAGATTAAGCGGCAGGCGCTAATGGATAATAATAATGTGCCTTCAAACAAAGGCAATGGGGTAAAAACCTTATTGTCCATTTTTATTCTGTTGAGTGATGTTGCTGTCTAATGTAGCATTGTTGGCAGTGTTTTATCACATTCAAATTTTCTTGTGTGCATTTACAGGAATTGCCTGATAGGGAGAAGGTTGATGCAACCCCTCCTTCTGAGGGGAAGAAGCAGAAGAAGATAGATACTGTTGACGAGGATGTTGACATCGGCGATGATATGCCAGCAAACAACTTCCCACCTGTGGAGATTGAGAAAGATAAAGATATGGGTGCTACTGGTGGCGGTGCCAGTAGTAGTTCCAGTGGCTCCAGCAGTTCTGGTAGTGATTCCTCGTCTAGTGGTATAACCTCTTTCCACTGAATGTTTTATGGTTTGTATTCTGAAATTATCATGGGTAGTAACCTCAATTTCGATACTTTTGCAGATTCGGATTCAGGGAGTTCTTCAGGAAGTGACTCTGAAGCGGGCAATGGTCATTTGTAG

Protein sequence

>MS.gene40087.t1

MASALLDNRNEPNWLQHRGGGAEFMGKAPNPNPKFSNKKRTQSPSDDASSINRRSNDNHSQYVTFNIESYTKTELYELKNRLVSELDQIRQLKTRIESGEFKPRLNHNGGGPTKKSGSKKVSGNKRPFPAVKELKKSKSEIGDAMKACGQILTKLMKNKSGWIFNTWIFNTPVNATALNLHDYFDIIKHPMDLGTVKSKLAKNAYSTPADFAADVKLTFNNALTYNPKGHDVNTAAKQLLEKFEELYRPIHEKFDEKSFDDELQASSWNHVEPERERERVKKKDNPIPIPPPVAKRQEPLPEPASTSNQPSTSNPPLAQSPVRIPSPTRALPVKPLKQPKPKARDPNKREMNVEEKHKLGLGLQILPPEKMEQVVQIIRKRNGHLEQDGDEIELDMEAVDTETLWELDRLVTNWKKMVSKIKRQALMDNNNVPSNKGNGELPDREKVDATPPSEGKKQKKIDTVDEDVDIGDDMPANNFPPVEIEKDKDMGATGGGASSSSSGSSSSGSDSSSSDSDSGSSSGSDSEAGNGHL