Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42372.t1 | GAU14324.1 | 85.2 | 88 | 11 | 2 | 4 | 90 | 1 | 87 | 5.90E-33 | 149.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42372.t1 | G7J4L6 | 85.1 | 87 | 7 | 1 | 4 | 90 | 1 | 81 | 2.8e-32 | 147.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene42372 | 0.815069 | 1.12E-51 | -1.69E-46 |
| MS.gene049356 | MS.gene42372 | 0.803665 | 3.13E-49 | -1.69E-46 |
| MS.gene049488 | MS.gene42372 | 0.807886 | 4.06E-50 | -1.69E-46 |
| MS.gene049609 | MS.gene42372 | 0.80732 | 5.35E-50 | -1.69E-46 |
| MS.gene049805 | MS.gene42372 | 0.842104 | 3.17E-58 | -1.69E-46 |
| MS.gene050593 | MS.gene42372 | 0.803608 | 3.21E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene42372 | 0.833914 | 4.05E-56 | -1.69E-46 |
| MS.gene050747 | MS.gene42372 | 0.802812 | 4.70E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene42372 | 0.809964 | 1.46E-50 | -1.69E-46 |
| MS.gene05105 | MS.gene42372 | 0.854517 | 1.17E-61 | -1.69E-46 |
| MS.gene052188 | MS.gene42372 | 0.80323 | 3.85E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene42372 | 0.809099 | 2.24E-50 | -1.69E-46 |
| MS.gene052421 | MS.gene42372 | 0.813161 | 2.95E-51 | -1.69E-46 |
| MS.gene052481 | MS.gene42372 | 0.814043 | 1.88E-51 | -1.69E-46 |
| MS.gene052484 | MS.gene42372 | 0.810746 | 9.89E-51 | -1.69E-46 |
| MS.gene052501 | MS.gene42372 | 0.803447 | 3.47E-49 | -1.69E-46 |
| MS.gene052505 | MS.gene42372 | 0.821393 | 4.14E-53 | -1.69E-46 |
| MS.gene052615 | MS.gene42372 | 0.825002 | 5.94E-54 | -1.69E-46 |
| MS.gene053103 | MS.gene42372 | 0.822836 | 1.91E-53 | -1.69E-46 |
| MS.gene053449 | MS.gene42372 | 0.800437 | 1.44E-48 | -1.69E-46 |
| MS.gene053579 | MS.gene42372 | 0.811713 | 6.10E-51 | -1.69E-46 |
| MS.gene053632 | MS.gene42372 | 0.80763 | 4.60E-50 | -1.69E-46 |
| MS.gene053912 | MS.gene42372 | 0.820359 | 7.15E-53 | -1.69E-46 |
| MS.gene053916 | MS.gene42372 | 0.807593 | 4.69E-50 | -1.69E-46 |
| MS.gene05505 | MS.gene42372 | 0.811128 | 8.17E-51 | -1.69E-46 |
| MS.gene055116 | MS.gene42372 | 0.815471 | 9.09E-52 | -1.69E-46 |
| MS.gene055206 | MS.gene42372 | 0.809786 | 1.59E-50 | -1.69E-46 |
| MS.gene055289 | MS.gene42372 | 0.809443 | 1.89E-50 | -1.69E-46 |
| MS.gene055941 | MS.gene42372 | 0.806583 | 7.67E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene42372 | 0.834012 | 3.83E-56 | -1.69E-46 |
| MS.gene057076 | MS.gene42372 | 0.849191 | 3.79E-60 | -1.69E-46 |
| MS.gene057124 | MS.gene42372 | 0.828923 | 6.86E-55 | -1.69E-46 |
| MS.gene057146 | MS.gene42372 | 0.824474 | 7.92E-54 | -1.69E-46 |
| MS.gene057416 | MS.gene42372 | 0.800158 | 1.64E-48 | -1.69E-46 |
| MS.gene057749 | MS.gene42372 | 0.802573 | 5.26E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene42372 | 0.814839 | 1.26E-51 | -1.69E-46 |
| MS.gene058019 | MS.gene42372 | 0.803131 | 4.03E-49 | -1.69E-46 |
| MS.gene058130 | MS.gene42372 | 0.804644 | 1.96E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene42372 | 0.825046 | 5.80E-54 | -1.69E-46 |
| MS.gene059366 | MS.gene42372 | 0.825532 | 4.45E-54 | -1.69E-46 |
| MS.gene059420 | MS.gene42372 | 0.809745 | 1.63E-50 | -1.69E-46 |
| MS.gene059455 | MS.gene42372 | 0.831102 | 2.02E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene42372 | 0.824972 | 6.04E-54 | -1.69E-46 |
| MS.gene059692 | MS.gene42372 | 0.812056 | 5.14E-51 | -1.69E-46 |
| MS.gene059695 | MS.gene42372 | 0.810311 | 1.23E-50 | -1.69E-46 |
| MS.gene060403 | MS.gene42372 | 0.803915 | 2.78E-49 | -1.69E-46 |
| MS.gene060818 | MS.gene42372 | 0.804657 | 1.94E-49 | -1.69E-46 |
| MS.gene060835 | MS.gene42372 | 0.808829 | 2.56E-50 | -1.69E-46 |
| MS.gene061041 | MS.gene42372 | 0.806698 | 7.25E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42372.t1 | MTR_3g111360 | 85.057 | 87 | 7 | 1 | 4 | 90 | 1 | 81 | 5.34e-47 | 145 |
| MS.gene42372.t1 | MTR_5g067050 | 65.714 | 70 | 20 | 3 | 22 | 89 | 13 | 80 | 1.47e-23 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAGGGTTGCTTTCATGCTT+TGG | 0.283837 | 3.1:-89006510 | MS.gene42372:CDS |
| TTAGCTAGCAATGGAAAGTT+TGG | 0.296785 | 3.1:-89006574 | None:intergenic |
| GAGGGTTGCTTTCATGCTTT+GGG | 0.333399 | 3.1:-89006509 | MS.gene42372:CDS |
| TTGTTTGTTCTCCTCTTCCA+TGG | 0.436490 | 3.1:+89006468 | None:intergenic |
| GAGGACGACGAAGAATCTTT+AGG | 0.441691 | 3.1:-89006369 | MS.gene42372:CDS |
| TCTCCTATAAGAAGCAAAAC+AGG | 0.462208 | 3.1:-89006537 | MS.gene42372:CDS |
| AGAAGCAAAACAGGTGGAGG+AGG | 0.521376 | 3.1:-89006528 | MS.gene42372:CDS |
| TGATGATGGTAATTTGGAGG+AGG | 0.522189 | 3.1:-89006388 | MS.gene42372:CDS |
| AACACTTATAGAACCGTTGG+TGG | 0.530511 | 3.1:-89006447 | MS.gene42372:CDS |
| AATTGAAGCTTCCATGGAAG+AGG | 0.536567 | 3.1:-89006479 | MS.gene42372:CDS |
| AACAACACTTATAGAACCGT+TGG | 0.561342 | 3.1:-89006450 | MS.gene42372:CDS |
| GTCTCAAATTGAAGCTTCCA+TGG | 0.569556 | 3.1:-89006485 | MS.gene42372:CDS |
| ATAAGAAGCAAAACAGGTGG+AGG | 0.576069 | 3.1:-89006531 | MS.gene42372:CDS |
| GACGAAGAATCTTTAGGTCG+CGG | 0.579606 | 3.1:-89006363 | MS.gene42372:CDS |
| ATCATACCTAAAACCACCAA+CGG | 0.621720 | 3.1:+89006434 | None:intergenic |
| TTGCTTCTTATAGGAGACGA+TGG | 0.630393 | 3.1:+89006543 | None:intergenic |
| GAAGCAAAACAGGTGGAGGA+GGG | 0.645020 | 3.1:-89006527 | MS.gene42372:CDS |
| CCTATAAGAAGCAAAACAGG+TGG | 0.657762 | 3.1:-89006534 | MS.gene42372:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTTGATGATGGTAATT+TGG | - | chr3.1:89006484-89006503 | MS.gene42372:CDS | 20.0% |
| !! | AAATTTTGAGCATAGCTTAA+AGG | + | chr3.1:89006470-89006489 | None:intergenic | 25.0% |
| !!! | TATGCTCAAAATTTTGATGA+TGG | - | chr3.1:89006476-89006495 | MS.gene42372:CDS | 25.0% |
| !!! | TTTTGATGATGGTAATTTGG+AGG | - | chr3.1:89006487-89006506 | MS.gene42372:CDS | 30.0% |
| AACAACACTTATAGAACCGT+TGG | - | chr3.1:89006428-89006447 | MS.gene42372:CDS | 35.0% | |
| ATCATACCTAAAACCACCAA+CGG | + | chr3.1:89006447-89006466 | None:intergenic | 35.0% | |
| TCTCCTATAAGAAGCAAAAC+AGG | - | chr3.1:89006341-89006360 | MS.gene42372:CDS | 35.0% | |
| AACACTTATAGAACCGTTGG+TGG | - | chr3.1:89006431-89006450 | MS.gene42372:CDS | 40.0% | |
| AATTGAAGCTTCCATGGAAG+AGG | - | chr3.1:89006399-89006418 | MS.gene42372:CDS | 40.0% | |
| ATAAGAAGCAAAACAGGTGG+AGG | - | chr3.1:89006347-89006366 | MS.gene42372:CDS | 40.0% | |
| CCTATAAGAAGCAAAACAGG+TGG | - | chr3.1:89006344-89006363 | MS.gene42372:CDS | 40.0% | |
| GTCTCAAATTGAAGCTTCCA+TGG | - | chr3.1:89006393-89006412 | MS.gene42372:CDS | 40.0% | |
| TTGCTTCTTATAGGAGACGA+TGG | + | chr3.1:89006338-89006357 | None:intergenic | 40.0% | |
| TTGTTTGTTCTCCTCTTCCA+TGG | + | chr3.1:89006413-89006432 | None:intergenic | 40.0% | |
| ! | CCACCTGTTTTGCTTCTTAT+AGG | + | chr3.1:89006347-89006366 | None:intergenic | 40.0% |
| !! | TGATGATGGTAATTTGGAGG+AGG | - | chr3.1:89006490-89006509 | MS.gene42372:CDS | 40.0% |
| !!! | ATAGAACCGTTGGTGGTTTT+AGG | - | chr3.1:89006438-89006457 | MS.gene42372:CDS | 40.0% |
| !!! | TGCTTGAGTGTTTTAGCTGA+AGG | + | chr3.1:89006563-89006582 | None:intergenic | 40.0% |
| GACGAAGAATCTTTAGGTCG+CGG | - | chr3.1:89006515-89006534 | MS.gene42372:CDS | 45.0% | |
| GAGGACGACGAAGAATCTTT+AGG | - | chr3.1:89006509-89006528 | MS.gene42372:CDS | 45.0% | |
| ! | GAGGGTTGCTTTCATGCTTT+GGG | - | chr3.1:89006369-89006388 | MS.gene42372:CDS | 45.0% |
| AGAAGCAAAACAGGTGGAGG+AGG | - | chr3.1:89006350-89006369 | MS.gene42372:CDS | 50.0% | |
| GAAGCAAAACAGGTGGAGGA+GGG | - | chr3.1:89006351-89006370 | MS.gene42372:CDS | 50.0% | |
| ! | GGAGGGTTGCTTTCATGCTT+TGG | - | chr3.1:89006368-89006387 | MS.gene42372:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 89006314 | 89006586 | 89006314 | ID=MS.gene42372 |
| chr3.1 | mRNA | 89006314 | 89006586 | 89006314 | ID=MS.gene42372.t1;Parent=MS.gene42372 |
| chr3.1 | exon | 89006314 | 89006586 | 89006314 | ID=MS.gene42372.t1.exon1;Parent=MS.gene42372.t1 |
| chr3.1 | CDS | 89006314 | 89006586 | 89006314 | ID=cds.MS.gene42372.t1;Parent=MS.gene42372.t1 |
| Gene Sequence |
| Protein sequence |
