Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53476.t1 | XP_003613218.1 | 100 | 808 | 0 | 0 | 1 | 808 | 1 | 808 | 0.00E+00 | 1636.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53476.t1 | P42347 | 92.9 | 814 | 52 | 1 | 1 | 808 | 1 | 814 | 0.0e+00 | 1529.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53476.t1 | Q8GUA6 | 100.0 | 808 | 0 | 0 | 1 | 808 | 1 | 808 | 0.0e+00 | 1636.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene53476 | 0.812933 | 3.30E-51 | -1.69E-46 |
| MS.gene049404 | MS.gene53476 | 0.808807 | 2.58E-50 | -1.69E-46 |
| MS.gene049435 | MS.gene53476 | 0.81322 | 2.86E-51 | -1.69E-46 |
| MS.gene050073 | MS.gene53476 | 0.801358 | 9.35E-49 | -1.69E-46 |
| MS.gene050306 | MS.gene53476 | 0.804496 | 2.10E-49 | -1.69E-46 |
| MS.gene050559 | MS.gene53476 | 0.821182 | 4.62E-53 | -1.69E-46 |
| MS.gene050561 | MS.gene53476 | 0.822214 | 2.67E-53 | -1.69E-46 |
| MS.gene050562 | MS.gene53476 | 0.815517 | 8.88E-52 | -1.69E-46 |
| MS.gene051198 | MS.gene53476 | 0.843092 | 1.73E-58 | -1.69E-46 |
| MS.gene051279 | MS.gene53476 | 0.829313 | 5.52E-55 | -1.69E-46 |
| MS.gene051744 | MS.gene53476 | 0.811979 | 5.34E-51 | -1.69E-46 |
| MS.gene051800 | MS.gene53476 | 0.821947 | 3.08E-53 | -1.69E-46 |
| MS.gene051859 | MS.gene53476 | 0.802137 | 6.47E-49 | -1.69E-46 |
| MS.gene05191 | MS.gene53476 | 0.800066 | 1.72E-48 | -1.69E-46 |
| MS.gene052239 | MS.gene53476 | -0.804722 | 1.88E-49 | -1.69E-46 |
| MS.gene053079 | MS.gene53476 | 0.802293 | 6.01E-49 | -1.69E-46 |
| MS.gene05308 | MS.gene53476 | 0.819605 | 1.06E-52 | -1.69E-46 |
| MS.gene053166 | MS.gene53476 | 0.806924 | 6.49E-50 | -1.69E-46 |
| MS.gene053541 | MS.gene53476 | 0.823564 | 1.29E-53 | -1.69E-46 |
| MS.gene053542 | MS.gene53476 | 0.83348 | 5.21E-56 | -1.69E-46 |
| MS.gene05364 | MS.gene53476 | 0.804829 | 1.79E-49 | -1.69E-46 |
| MS.gene053931 | MS.gene53476 | 0.811068 | 8.42E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene53476 | 0.803818 | 2.91E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene53476 | 0.801804 | 7.58E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene53476 | 0.839779 | 1.29E-57 | -1.69E-46 |
| MS.gene055228 | MS.gene53476 | 0.805214 | 1.49E-49 | -1.69E-46 |
| MS.gene055586 | MS.gene53476 | 0.80599 | 1.02E-49 | -1.69E-46 |
| MS.gene056050 | MS.gene53476 | 0.800193 | 1.62E-48 | -1.69E-46 |
| MS.gene056252 | MS.gene53476 | 0.8001 | 1.69E-48 | -1.69E-46 |
| MS.gene056507 | MS.gene53476 | 0.802363 | 5.81E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene53476 | 0.854466 | 1.21E-61 | -1.69E-46 |
| MS.gene056973 | MS.gene53476 | 0.808472 | 3.05E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene53476 | 0.827316 | 1.67E-54 | -1.69E-46 |
| MS.gene057598 | MS.gene53476 | 0.831499 | 1.61E-55 | -1.69E-46 |
| MS.gene057869 | MS.gene53476 | 0.845428 | 4.09E-59 | -1.69E-46 |
| MS.gene058009 | MS.gene53476 | 0.829708 | 4.43E-55 | -1.69E-46 |
| MS.gene058010 | MS.gene53476 | 0.82952 | 4.92E-55 | -1.69E-46 |
| MS.gene058781 | MS.gene53476 | 0.837272 | 5.73E-57 | -1.69E-46 |
| MS.gene058783 | MS.gene53476 | 0.820709 | 5.94E-53 | -1.69E-46 |
| MS.gene058919 | MS.gene53476 | 0.809859 | 1.54E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene53476 | 0.847159 | 1.38E-59 | -1.69E-46 |
| MS.gene059317 | MS.gene53476 | 0.805507 | 1.29E-49 | -1.69E-46 |
| MS.gene059842 | MS.gene53476 | 0.800646 | 1.31E-48 | -1.69E-46 |
| MS.gene060310 | MS.gene53476 | 0.806024 | 1.01E-49 | -1.69E-46 |
| MS.gene060918 | MS.gene53476 | 0.810852 | 9.38E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene53476 | 0.822889 | 1.86E-53 | -1.69E-46 |
| MS.gene060937 | MS.gene53476 | 0.814782 | 1.29E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene53476 | MS.gene059541 | PPI |
| MS.gene53476 | MS.gene66405 | PPI |
| MS.gene53476 | MS.gene66413 | PPI |
| MS.gene53476 | MS.gene063319 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53476.t1 | MTR_5g034120 | 100.000 | 808 | 0 | 0 | 1 | 808 | 1 | 808 | 0.0 | 1670 |
| MS.gene53476.t1 | MTR_3g116250 | 28.778 | 483 | 293 | 19 | 334 | 788 | 1555 | 2014 | 2.81e-34 | 142 |
| MS.gene53476.t1 | MTR_8g093570 | 33.906 | 233 | 141 | 6 | 555 | 777 | 829 | 1058 | 2.90e-27 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53476.t1 | AT1G60490 | 84.049 | 815 | 122 | 4 | 1 | 808 | 1 | 814 | 0.0 | 1415 |
| MS.gene53476.t1 | AT1G49340 | 27.477 | 535 | 336 | 19 | 274 | 781 | 1491 | 2000 | 3.01e-38 | 155 |
| MS.gene53476.t1 | AT1G49340 | 27.477 | 535 | 336 | 19 | 274 | 781 | 1491 | 2000 | 3.01e-38 | 155 |
| MS.gene53476.t1 | AT1G49340 | 27.477 | 535 | 336 | 19 | 274 | 781 | 1484 | 1993 | 3.04e-38 | 155 |
| MS.gene53476.t1 | AT1G49340 | 27.477 | 535 | 336 | 19 | 274 | 781 | 1484 | 1993 | 3.04e-38 | 155 |
| MS.gene53476.t1 | AT5G64070 | 33.617 | 235 | 139 | 7 | 555 | 777 | 861 | 1090 | 5.33e-28 | 122 |
| MS.gene53476.t1 | AT5G09350 | 33.906 | 233 | 141 | 6 | 555 | 777 | 856 | 1085 | 8.18e-28 | 121 |
| MS.gene53476.t1 | AT1G51040 | 28.956 | 297 | 178 | 11 | 511 | 788 | 222 | 504 | 1.95e-19 | 93.2 |
Find 182 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTAAAATAGATTTAAAATT+AGG | 0.176506 | 5.3:-23170630 | None:intergenic |
| AAATAAATATACCTGAATTA+AGG | 0.196555 | 5.3:-23160673 | None:intergenic |
| CCTGATGATCATGGGCCTTT+TGG | 0.206214 | 5.3:+23169080 | MS.gene53476:CDS |
| TAGATTTAAAATTAGGTTAC+TGG | 0.212396 | 5.3:-23170623 | None:intergenic |
| TGTTCAAATGGTATCGTTAA+TGG | 0.238019 | 5.3:+23168135 | MS.gene53476:CDS |
| AAGCTTAGACAGCTACTTTC+TGG | 0.244205 | 5.3:+23167545 | MS.gene53476:CDS |
| GCCAGTCCACTCGTTGAATT+TGG | 0.248295 | 5.3:-23163378 | None:intergenic |
| ATAGCTTCTGATCCTGAAAA+AGG | 0.252329 | 5.3:+23170679 | MS.gene53476:CDS |
| TTCATCGTTGGGCTCAATAT+TGG | 0.265072 | 5.3:+23170897 | MS.gene53476:CDS |
| CACATTGATGGCGCGCCGTT+TGG | 0.265882 | 5.3:+23161077 | MS.gene53476:CDS |
| TATACCTGAATTAAGGGAAT+TGG | 0.270070 | 5.3:-23160666 | None:intergenic |
| ACTTGAATCTTGTATAATAT+TGG | 0.275932 | 5.3:-23170564 | None:intergenic |
| TTTCCCAATTCCCTTAATTC+AGG | 0.292418 | 5.3:+23160662 | MS.gene53476:CDS |
| TTACTGCACATATGAAATTC+TGG | 0.297436 | 5.3:+23166913 | MS.gene53476:CDS |
| AGAAGCTATGTCCGGAATAT+TGG | 0.311004 | 5.3:-23170665 | None:intergenic |
| ATGACCCTACATATGCAAAA+AGG | 0.322562 | 5.3:+23166888 | MS.gene53476:CDS |
| TGCACATTATTAACCTTTCC+AGG | 0.328164 | 5.3:-23163190 | None:intergenic |
| TCACGTTAACAGCTAAATAT+CGG | 0.339699 | 5.3:+23162763 | MS.gene53476:CDS |
| AGCTTCATTGGTGGCGGAAA+TGG | 0.339940 | 5.3:-23170399 | None:intergenic |
| AATAAATATACCTGAATTAA+GGG | 0.341109 | 5.3:-23160672 | None:intergenic |
| AATTTCTTCGTTGTGTTGAA+TGG | 0.348338 | 5.3:+23165372 | MS.gene53476:CDS |
| ATTTCCTTGCAGAGCTTCAT+TGG | 0.349240 | 5.3:-23170411 | None:intergenic |
| TTACTTGCTCCAATTGGTTC+AGG | 0.349986 | 5.3:+23166663 | MS.gene53476:CDS |
| AGCATTAAGCAATATTGAAT+TGG | 0.350124 | 5.3:+23166829 | MS.gene53476:intron |
| GACCAATACCTCATCAAAAT+AGG | 0.353005 | 5.3:-23167585 | None:intergenic |
| AAAGACTTCCATCGTCTCTT+AGG | 0.355332 | 5.3:-23170335 | None:intergenic |
| CCATACAAGGTTCTAGCAAC+TGG | 0.356646 | 5.3:+23168199 | MS.gene53476:CDS |
| CCAATGATATTGTTATTGTC+TGG | 0.358804 | 5.3:+23164428 | MS.gene53476:CDS |
| ATTTAAACGGTGGCAGAGTT+TGG | 0.367837 | 5.3:+23167436 | MS.gene53476:CDS |
| GGAAGCAAAAGCTTAGGCTT+TGG | 0.380644 | 5.3:+23163128 | MS.gene53476:CDS |
| GGAAAAGTTTCGGTTGGACT+TGG | 0.385873 | 5.3:+23170793 | MS.gene53476:CDS |
| ATCACATATATACTTGGTAT+TGG | 0.386539 | 5.3:+23169158 | MS.gene53476:CDS |
| TCTCATGGTGAAGGATTGAT+TGG | 0.388746 | 5.3:+23163058 | MS.gene53476:CDS |
| GAAGCAAAAGCTTAGGCTTT+GGG | 0.388788 | 5.3:+23163129 | MS.gene53476:CDS |
| TCGCCTCACGTTCAAAACTA+TGG | 0.389595 | 5.3:+23163405 | MS.gene53476:CDS |
| TTAGGTCCCTAGGCATGAAC+GGG | 0.395425 | 5.3:+23163309 | MS.gene53476:intron |
| TCCAATTGGTTCAGGCTCTT+CGG | 0.397784 | 5.3:+23166671 | MS.gene53476:CDS |
| ATCCCAGTGATGAGCACATC+TGG | 0.403063 | 5.3:-23167791 | None:intergenic |
| TACTTTCTCTCATTTGAGCT+TGG | 0.405483 | 5.3:-23164544 | None:intergenic |
| CTTGTTGCAGAAAATGATGA+TGG | 0.410431 | 5.3:+23161023 | MS.gene53476:intron |
| GAAAGGGCAAATTGCCTTCA+AGG | 0.411531 | 5.3:-23160643 | None:intergenic |
| GGCTCTTCGGTTCGAGAGAT+CGG | 0.414772 | 5.3:+23166684 | MS.gene53476:CDS |
| CCCTACCTGTGCTAATGAAC+GGG | 0.416972 | 5.3:-23168254 | None:intergenic |
| TTCTTTGATGTCTGAGAAGA+GGG | 0.418191 | 5.3:+23165340 | MS.gene53476:CDS |
| GATGGCGGGTTCCAATATTC+CGG | 0.423699 | 5.3:+23170654 | MS.gene53476:CDS |
| TACAGCTCCAGGAAAAGTTT+CGG | 0.427922 | 5.3:+23170783 | MS.gene53476:intron |
| TTCCGCATCGAACGCCTTGA+AGG | 0.429980 | 5.3:+23160629 | MS.gene53476:CDS |
| CCAGTTGCTAGAACCTTGTA+TGG | 0.430909 | 5.3:-23168199 | None:intergenic |
| ACACCAGATGTGCTCATCAC+TGG | 0.432555 | 5.3:+23167788 | MS.gene53476:CDS |
| ATACCTGAATTAAGGGAATT+GGG | 0.434366 | 5.3:-23160665 | None:intergenic |
| ATTGGTGGCGGAAATGGCTT+AGG | 0.435687 | 5.3:-23170393 | None:intergenic |
| GTTGTATGCTTCACAACAAT+AGG | 0.437263 | 5.3:-23170587 | None:intergenic |
| GAAACAAGCACTAGAATTGA+TGG | 0.437691 | 5.3:+23165924 | MS.gene53476:CDS |
| ATATCATTGGTCGAAGCTAT+AGG | 0.441914 | 5.3:-23164415 | None:intergenic |
| CAAGGAAATGGTTGAGGCTA+TGG | 0.443471 | 5.3:+23170425 | MS.gene53476:CDS |
| AGCCTGTATACATTTCTTCC+AGG | 0.443637 | 5.3:+23170823 | MS.gene53476:CDS |
| AATTGGCAAGCTTTCTTCGC+TGG | 0.446366 | 5.3:+23166846 | MS.gene53476:CDS |
| AATGAAGCTCTGCAAGGAAA+TGG | 0.448445 | 5.3:+23170413 | MS.gene53476:CDS |
| CCCTAACAATTTCACATGAC+TGG | 0.450078 | 5.3:+23160557 | None:intergenic |
| GAGTGTGCGTTGCACATTGA+TGG | 0.450351 | 5.3:+23161065 | MS.gene53476:CDS |
| TCAATGTTACTTGCTCCAAT+TGG | 0.451060 | 5.3:+23166657 | MS.gene53476:CDS |
| CCAAAAGGCCCATGATCATC+AGG | 0.453310 | 5.3:-23169080 | None:intergenic |
| CGCTCTCGTTGATAAGATCC+TGG | 0.454719 | 5.3:-23170841 | None:intergenic |
| GTGAAGCATACAACATTCTT+CGG | 0.454783 | 5.3:+23170596 | MS.gene53476:CDS |
| GGTTGAGACCATTCATCGTT+GGG | 0.455522 | 5.3:+23170886 | MS.gene53476:CDS |
| CCACAGGACCCTCGTATTGT+TGG | 0.459987 | 5.3:+23162730 | MS.gene53476:CDS |
| CCAGACAATAACAATATCAT+TGG | 0.466559 | 5.3:-23164428 | None:intergenic |
| ACCGAAGAGCCTGAACCAAT+TGG | 0.466842 | 5.3:-23166672 | None:intergenic |
| CAATGATATTGTTATTGTCT+GGG | 0.467655 | 5.3:+23164429 | MS.gene53476:CDS |
| GCAGGAATACCAACTGGTCC+TGG | 0.471798 | 5.3:-23167928 | None:intergenic |
| TATAAACGTTTCAAGACAGT+TGG | 0.477924 | 5.3:-23169111 | None:intergenic |
| GGACTTTGAGTGGAGATGAT+AGG | 0.478943 | 5.3:+23165294 | MS.gene53476:CDS |
| CCTGCATTTAACTCCATACA+AGG | 0.479213 | 5.3:+23168186 | MS.gene53476:CDS |
| TGGGATCTTTCTCATGGTGA+AGG | 0.482025 | 5.3:+23163049 | MS.gene53476:CDS |
| TCTCCACTCAAAGTCCTTGT+TGG | 0.482283 | 5.3:-23165287 | None:intergenic |
| GCCAAATTCAACGAGTGGAC+TGG | 0.484095 | 5.3:+23163377 | MS.gene53476:CDS |
| CACGTTAACAGCTAAATATC+GGG | 0.491737 | 5.3:+23162764 | MS.gene53476:CDS |
| TCAGGATCAGAAGCTATGTC+CGG | 0.491939 | 5.3:-23170673 | None:intergenic |
| CAGGTTTGGGATCTTTCTCA+TGG | 0.498015 | 5.3:+23163043 | MS.gene53476:intron |
| CACCAGATGTGCTCATCACT+GGG | 0.498800 | 5.3:+23167789 | MS.gene53476:CDS |
| ATCCTGGAAGAAATGTATAC+AGG | 0.501151 | 5.3:-23170825 | None:intergenic |
| ACCAAGTATATATGTGATAA+CGG | 0.501900 | 5.3:-23169153 | None:intergenic |
| GACACCACGGGTTAAACTCC+TGG | 0.502225 | 5.3:-23164503 | None:intergenic |
| TGTGTCCTCATTAGGTCCCT+AGG | 0.503314 | 5.3:+23163299 | MS.gene53476:intron |
| TGGTGAAGGTTGGAGTCCAC+AGG | 0.504014 | 5.3:+23162714 | MS.gene53476:intron |
| ACCTTTCCAGGTGTAGAAGT+AGG | 0.504443 | 5.3:-23163178 | None:intergenic |
| AAGATGATATAACCTTGTTC+TGG | 0.504774 | 5.3:-23161108 | None:intergenic |
| ATATTGAGCCCAACGATGAA+TGG | 0.507424 | 5.3:-23170894 | None:intergenic |
| TTTAACTGTTTATGCTCAGA+GGG | 0.508499 | 5.3:-23164475 | None:intergenic |
| CCTTTCCAGGTGTAGAAGTA+GGG | 0.509565 | 5.3:-23163177 | None:intergenic |
| ATACTTCAATATTCTCTGAA+TGG | 0.509593 | 5.3:-23165261 | None:intergenic |
| TCCATCCCGTTCATTAGCAC+AGG | 0.513341 | 5.3:+23168249 | MS.gene53476:CDS |
| ATAAACAGTTAAAGCTGGCC+AGG | 0.513701 | 5.3:+23164485 | MS.gene53476:CDS |
| CGTTCGATGCGGAAAGTGAC+GGG | 0.517013 | 5.3:-23160620 | None:intergenic |
| AAGCTTTCTTCGCTGGTATG+TGG | 0.517061 | 5.3:+23166853 | MS.gene53476:CDS |
| GGTGCAAACGGAGAGGAAGA+TGG | 0.517256 | 5.3:+23167413 | MS.gene53476:CDS |
| CTAGCAACTGGGCAAGATGA+AGG | 0.518120 | 5.3:+23168211 | MS.gene53476:CDS |
| CAAAACTATGGAGAAAATCA+AGG | 0.519164 | 5.3:+23163417 | MS.gene53476:CDS |
| CCACTCAAAGTCCTTGTTGG+TGG | 0.523782 | 5.3:-23165284 | None:intergenic |
| CAAAAGCTTAGGCTTTGGGC+AGG | 0.524104 | 5.3:+23163133 | MS.gene53476:CDS |
| TTAAATATTGATGACTCTGA+GGG | 0.524865 | 5.3:-23167818 | None:intergenic |
| CAGATGGGAGCTGGTGCAAA+CGG | 0.525919 | 5.3:+23167401 | MS.gene53476:intron |
| TCCGTTATCACATATATACT+TGG | 0.530140 | 5.3:+23169152 | MS.gene53476:CDS |
| TCCTTGCAGAGCTTCATTGG+TGG | 0.531959 | 5.3:-23170408 | None:intergenic |
| TGTTGAATGGAGTGATGTAC+AGG | 0.532745 | 5.3:+23165385 | MS.gene53476:CDS |
| CCCTACTTCTACACCTGGAA+AGG | 0.534797 | 5.3:+23163177 | MS.gene53476:CDS |
| CAGTTGGCTGTAATTCCAAA+AGG | 0.534886 | 5.3:-23169095 | None:intergenic |
| TTCCATCGTCTCTTAGGAGG+AGG | 0.535524 | 5.3:-23170329 | None:intergenic |
| TTTCTTTGATGTCTGAGAAG+AGG | 0.535885 | 5.3:+23165339 | MS.gene53476:CDS |
| TTGCAGAGCTTCATTGGTGG+CGG | 0.538044 | 5.3:-23170405 | None:intergenic |
| GAGTGCATTGTTCCCTCAGA+TGG | 0.538202 | 5.3:+23170865 | MS.gene53476:CDS |
| GATTGACGTTTGTGATGCAC+TGG | 0.539342 | 5.3:+23165960 | MS.gene53476:CDS |
| CCAAAATCTCAGATGGGAGC+TGG | 0.541918 | 5.3:+23167392 | MS.gene53476:intron |
| CACTAGAATTGATGGGCAAG+TGG | 0.544415 | 5.3:+23165932 | MS.gene53476:CDS |
| GCTCCAGGAAAAGTTTCGGT+TGG | 0.551085 | 5.3:+23170787 | MS.gene53476:intron |
| GTTCAATCACTAGAGATGTC+AGG | 0.551302 | 5.3:+23167489 | MS.gene53476:CDS |
| ATGATATAACCTTGTTCTGG+TGG | 0.552076 | 5.3:-23161105 | None:intergenic |
| ATTAGGTCCCTAGGCATGAA+CGG | 0.552317 | 5.3:+23163308 | MS.gene53476:intron |
| GGTGTCAGAGGTGATCGTAT+TGG | 0.554165 | 5.3:-23167770 | None:intergenic |
| TGAATGGTCTCAACCATCTG+AGG | 0.555119 | 5.3:-23170878 | None:intergenic |
| TGAATTAAGGGAATTGGGAA+AGG | 0.555390 | 5.3:-23160660 | None:intergenic |
| GTTCTGGTGGGAAGTCCAAA+CGG | 0.556275 | 5.3:-23161092 | None:intergenic |
| AAGGAAATGGTTGAGGCTAT+GGG | 0.556476 | 5.3:+23170426 | MS.gene53476:CDS |
| GCGTTCGATGCGGAAAGTGA+CGG | 0.556749 | 5.3:-23160621 | None:intergenic |
| CATGGTGAAGGATTGATTGG+AGG | 0.557450 | 5.3:+23163061 | MS.gene53476:CDS |
| TTTAAATATTGATGACTCTG+AGG | 0.558343 | 5.3:-23167819 | None:intergenic |
| GAGAGGAAGATGGATTTAAA+CGG | 0.558804 | 5.3:+23167423 | MS.gene53476:CDS |
| AGGCTATGGGTGGAGCTGAA+AGG | 0.561462 | 5.3:+23170439 | MS.gene53476:CDS |
| ATATACTTGGTATTGGAGAC+AGG | 0.562402 | 5.3:+23169165 | MS.gene53476:CDS |
| AGACTGGGAAGCAAAAGCTT+AGG | 0.563123 | 5.3:+23163122 | MS.gene53476:CDS |
| ACCCTACCTGTGCTAATGAA+CGG | 0.563767 | 5.3:-23168255 | None:intergenic |
| TGAGCATAAACAGTTAAAGC+TGG | 0.564717 | 5.3:+23164480 | MS.gene53476:CDS |
| CCTTGTATGGAGTTAAATGC+AGG | 0.566490 | 5.3:-23168186 | None:intergenic |
| GAATTAAGGGAATTGGGAAA+GGG | 0.568755 | 5.3:-23160659 | None:intergenic |
| TGGTTGAGACCATTCATCGT+TGG | 0.569134 | 5.3:+23170885 | MS.gene53476:CDS |
| TCTGGAGGAGTATATGATGA+AGG | 0.572902 | 5.3:+23166931 | MS.gene53476:CDS |
| TGGGCAAGATGAAGGCATGT+TGG | 0.574569 | 5.3:+23168219 | MS.gene53476:CDS |
| GCTCTGCAAGGAAATGGTTG+AGG | 0.577513 | 5.3:+23170419 | MS.gene53476:CDS |
| GACTTCCATCGTCTCTTAGG+AGG | 0.577553 | 5.3:-23170332 | None:intergenic |
| GTTGTTAAAGAGAAGAATTG+TGG | 0.583980 | 5.3:-23163086 | None:intergenic |
| TGCCTTCAAGGCGTTCGATG+CGG | 0.584785 | 5.3:-23160631 | None:intergenic |
| GGTCCCTAGGCATGAACGGG+GGG | 0.585456 | 5.3:+23163312 | MS.gene53476:intron |
| TATTGTCTGGGACCCAGAAG+TGG | 0.588125 | 5.3:+23164441 | MS.gene53476:CDS |
| TCTTCTTCTGTGTATTACCT+CGG | 0.588947 | 5.3:-23167517 | None:intergenic |
| CTTTAACTGTTTATGCTCAG+AGG | 0.591757 | 5.3:-23164476 | None:intergenic |
| ATCTCTGTCTATGACACCAC+GGG | 0.595270 | 5.3:-23164515 | None:intergenic |
| TGGAAGTGCTCATTTGTATG+TGG | 0.595283 | 5.3:+23163459 | MS.gene53476:CDS |
| TGATCTTCGCCAGGACCAGT+TGG | 0.597803 | 5.3:+23167919 | MS.gene53476:CDS |
| ACTAGAATTGATGGGCAAGT+GGG | 0.598933 | 5.3:+23165933 | MS.gene53476:CDS |
| TTGGTCGAAGCTATAGGAGA+TGG | 0.600468 | 5.3:-23164409 | None:intergenic |
| TTGGACTTCCCACCAGAACA+AGG | 0.604060 | 5.3:+23161096 | MS.gene53476:CDS |
| CCACCAACAAGGACTTTGAG+TGG | 0.604075 | 5.3:+23165284 | MS.gene53476:CDS |
| GAGATGATAGGCAACTCTTG+TGG | 0.604236 | 5.3:+23165306 | MS.gene53476:CDS |
| TGATATAACCTTGTTCTGGT+GGG | 0.608995 | 5.3:-23161104 | None:intergenic |
| ACATTCCCTACTTCTACACC+TGG | 0.610568 | 5.3:+23163172 | MS.gene53476:CDS |
| CATACAAGGTTCTAGCAACT+GGG | 0.611041 | 5.3:+23168200 | MS.gene53476:CDS |
| AAACAAGCACTAGAATTGAT+GGG | 0.614052 | 5.3:+23165925 | MS.gene53476:CDS |
| TGGGCAGGAAAAGAAGCAGA+TGG | 0.617410 | 5.3:+23163148 | MS.gene53476:CDS |
| ACCTGTGCTAATGAACGGGA+TGG | 0.618260 | 5.3:-23168250 | None:intergenic |
| AACCTCCTCCTAAGAGACGA+TGG | 0.624022 | 5.3:+23170327 | MS.gene53476:CDS |
| ATTGTCTGGGACCCAGAAGT+GGG | 0.631740 | 5.3:+23164442 | MS.gene53476:CDS |
| GCCACCAATGAAGCTCTGCA+AGG | 0.636272 | 5.3:+23170407 | MS.gene53476:CDS |
| TGGAACTGCAGAGTTATATG+TGG | 0.636425 | 5.3:+23161043 | MS.gene53476:CDS |
| AGGTCCCTAGGCATGAACGG+GGG | 0.637589 | 5.3:+23163311 | MS.gene53476:intron |
| GAATAAAACCAAAATCAACA+TGG | 0.639157 | 5.3:-23170359 | None:intergenic |
| GAGCTCATTCCAACAATACG+AGG | 0.645146 | 5.3:-23162739 | None:intergenic |
| GCGAGGCCAAATTCAACGAG+TGG | 0.647883 | 5.3:+23163372 | MS.gene53476:CDS |
| GAACACTCACTGCATAAGCA+CGG | 0.655020 | 5.3:-23166609 | None:intergenic |
| GAAATGGTTGAGGCTATGGG+TGG | 0.659442 | 5.3:+23170429 | MS.gene53476:CDS |
| TAGGTCCCTAGGCATGAACG+GGG | 0.661850 | 5.3:+23163310 | MS.gene53476:intron |
| ATGAGCACATCTGGTGTCAG+AGG | 0.665902 | 5.3:-23167782 | None:intergenic |
| GAATGGTCTCAACCATCTGA+GGG | 0.666217 | 5.3:-23170877 | None:intergenic |
| CTGCACATATGAAATTCTGG+AGG | 0.666533 | 5.3:+23166916 | MS.gene53476:CDS |
| ACCCCATGCACATACCACGC+TGG | 0.667482 | 5.3:-23166735 | None:intergenic |
| GGGAGCTGGTGCAAACGGAG+AGG | 0.679178 | 5.3:+23167406 | MS.gene53476:CDS |
| CCAACAATACGAGGGTCCTG+TGG | 0.680529 | 5.3:-23162730 | None:intergenic |
| AGGAAGATGGATTTAAACGG+TGG | 0.680685 | 5.3:+23167426 | MS.gene53476:CDS |
| GATCTCTGTCTATGACACCA+CGG | 0.681065 | 5.3:-23164516 | None:intergenic |
| TATTGAAGTATCCACCAACA+AGG | 0.699350 | 5.3:+23165273 | MS.gene53476:CDS |
| AGAGATGTCAGGAATGTCCG+AGG | 0.705449 | 5.3:+23167500 | MS.gene53476:CDS |
| AGCTCATTCCAACAATACGA+GGG | 0.708520 | 5.3:-23162738 | None:intergenic |
| CTGGCCAGGAGTTTAACCCG+TGG | 0.725449 | 5.3:+23164499 | MS.gene53476:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 23160572 | 23170925 | 23160572 | ID=MS.gene53476 |
| chr5.3 | mRNA | 23160572 | 23170925 | 23160572 | ID=MS.gene53476.t1;Parent=MS.gene53476 |
| chr5.3 | exon | 23160572 | 23160683 | 23160572 | ID=MS.gene53476.t1.exon1;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23160572 | 23160683 | 23160572 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23161033 | 23161117 | 23161033 | ID=MS.gene53476.t1.exon2;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23161033 | 23161117 | 23161033 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23162722 | 23162818 | 23162722 | ID=MS.gene53476.t1.exon3;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23162722 | 23162818 | 23162722 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23163046 | 23163198 | 23163046 | ID=MS.gene53476.t1.exon4;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23163046 | 23163198 | 23163046 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23163313 | 23163522 | 23163313 | ID=MS.gene53476.t1.exon5;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23163313 | 23163522 | 23163313 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23164385 | 23164563 | 23164385 | ID=MS.gene53476.t1.exon6;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23164385 | 23164563 | 23164385 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23165259 | 23165406 | 23165259 | ID=MS.gene53476.t1.exon7;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23165259 | 23165406 | 23165259 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23165919 | 23166014 | 23165919 | ID=MS.gene53476.t1.exon8;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23165919 | 23166014 | 23165919 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23166607 | 23166742 | 23166607 | ID=MS.gene53476.t1.exon9;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23166607 | 23166742 | 23166607 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23166831 | 23166952 | 23166831 | ID=MS.gene53476.t1.exon10;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23166831 | 23166952 | 23166831 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23167404 | 23167598 | 23167404 | ID=MS.gene53476.t1.exon11;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23167404 | 23167598 | 23167404 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23167770 | 23167940 | 23167770 | ID=MS.gene53476.t1.exon12;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23167770 | 23167940 | 23167770 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23168133 | 23168270 | 23168133 | ID=MS.gene53476.t1.exon13;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23168133 | 23168270 | 23168133 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23169032 | 23169186 | 23169032 | ID=MS.gene53476.t1.exon14;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23169032 | 23169186 | 23169032 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23170317 | 23170460 | 23170317 | ID=MS.gene53476.t1.exon15;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23170317 | 23170460 | 23170317 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23170564 | 23170711 | 23170564 | ID=MS.gene53476.t1.exon16;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23170564 | 23170711 | 23170564 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| chr5.3 | exon | 23170788 | 23170925 | 23170788 | ID=MS.gene53476.t1.exon17;Parent=MS.gene53476.t1 |
| chr5.3 | CDS | 23170788 | 23170925 | 23170788 | ID=cds.MS.gene53476.t1;Parent=MS.gene53476.t1 |
| Gene Sequence |
| Protein sequence |
