Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05447.t1 | XP_013453000.1 | 95.2 | 557 | 27 | 0 | 3 | 559 | 47 | 603 | 9.20E-233 | 816.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05447.t1 | Q6Q151 | 75.4 | 374 | 82 | 5 | 1 | 371 | 45 | 411 | 3.0e-147 | 523.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05447.t1 | G7KN14 | 95.2 | 557 | 27 | 0 | 3 | 559 | 64 | 620 | 6.6e-233 | 816.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049404 | MS.gene05447 | 0.806271 | 8.92E-50 | -1.69E-46 |
| MS.gene049435 | MS.gene05447 | 0.803477 | 3.42E-49 | -1.69E-46 |
| MS.gene05157 | MS.gene05447 | 0.803261 | 3.79E-49 | -1.69E-46 |
| MS.gene052611 | MS.gene05447 | 0.800199 | 1.61E-48 | -1.69E-46 |
| MS.gene052863 | MS.gene05447 | 0.803654 | 3.14E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene054651 | 0.806752 | 7.06E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene056173 | 0.814131 | 1.80E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene057869 | 0.811636 | 6.34E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene063060 | 0.803215 | 3.88E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene063407 | 0.802929 | 4.44E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene065825 | 0.817545 | 3.12E-52 | -1.69E-46 |
| MS.gene05447 | MS.gene066320 | 0.815901 | 7.29E-52 | -1.69E-46 |
| MS.gene05447 | MS.gene067163 | 0.814865 | 1.24E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene06723 | 0.815076 | 1.11E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene06940 | 0.805626 | 1.22E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene070943 | 0.800289 | 1.55E-48 | -1.69E-46 |
| MS.gene05447 | MS.gene071457 | 0.813578 | 2.38E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene07530 | 0.81672 | 4.78E-52 | -1.69E-46 |
| MS.gene05447 | MS.gene08577 | 0.812842 | 3.46E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene21927 | 0.825238 | 5.23E-54 | -1.69E-46 |
| MS.gene05447 | MS.gene21929 | 0.81992 | 9.01E-53 | -1.69E-46 |
| MS.gene05447 | MS.gene22271 | 0.806153 | 9.45E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene23030 | 0.85199 | 6.19E-61 | -1.69E-46 |
| MS.gene05447 | MS.gene23528 | 0.801978 | 6.98E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene24067 | 0.801776 | 7.68E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene27302 | 0.805715 | 1.17E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene27971 | 0.810472 | 1.13E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene27972 | 0.808246 | 3.40E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene28854 | 0.802838 | 4.64E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene29022 | 0.822302 | 2.55E-53 | -1.69E-46 |
| MS.gene05447 | MS.gene30739 | 0.807356 | 5.26E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene30930 | 0.817501 | 3.19E-52 | -1.69E-46 |
| MS.gene05447 | MS.gene31652 | 0.807471 | 4.97E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene33624 | 0.801537 | 8.59E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene34369 | 0.805617 | 1.22E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene35631 | 0.813385 | 2.63E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene36132 | 0.802401 | 5.71E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene45918 | 0.809433 | 1.90E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene47603 | 0.814696 | 1.35E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene49423 | 0.808528 | 2.96E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene51459 | 0.810435 | 1.15E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene51511 | 0.803173 | 3.96E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene51818 | 0.809536 | 1.80E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene53064 | 0.806793 | 6.92E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene53467 | 0.812819 | 3.50E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene53476 | 0.801804 | 7.58E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene53586 | 0.832662 | 8.32E-56 | -1.69E-46 |
| MS.gene05447 | MS.gene56562 | 0.812778 | 3.57E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene58686 | 0.805012 | 1.64E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene59388 | 0.817688 | 2.90E-52 | -1.69E-46 |
| MS.gene05447 | MS.gene62214 | 0.808492 | 3.02E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene62250 | 0.805843 | 1.10E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene63434 | 0.811394 | 7.15E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene64249 | 0.845524 | 3.85E-59 | -1.69E-46 |
| MS.gene05447 | MS.gene65271 | 0.803796 | 2.94E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene67746 | 0.80937 | 1.96E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene70895 | 0.812934 | 3.30E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene72268 | 0.842716 | 2.18E-58 | -1.69E-46 |
| MS.gene05447 | MS.gene72555 | 0.807191 | 5.70E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene78775 | 0.800157 | 1.64E-48 | -1.69E-46 |
| MS.gene05447 | MS.gene82699 | 0.81465 | 1.38E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene86844 | 0.841368 | 4.96E-58 | -1.69E-46 |
| MS.gene05447 | MS.gene88243 | 0.810907 | 9.13E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene88304 | 0.800787 | 1.22E-48 | -1.69E-46 |
| MS.gene05447 | MS.gene92383 | 0.837816 | 4.16E-57 | -1.69E-46 |
| MS.gene05447 | MS.gene95698 | 0.826443 | 2.71E-54 | -1.69E-46 |
| MS.gene05447 | MS.gene95981 | 0.812354 | 4.42E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene98611 | 0.805647 | 1.21E-49 | -1.69E-46 |
| MS.gene05447 | MS.gene98828 | 0.835755 | 1.40E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene020412 | MS.gene05447 | PPI |
| MS.gene052174 | MS.gene05447 | PPI |
| MS.gene05447 | MS.gene031671 | PPI |
| MS.gene31419 | MS.gene05447 | PPI |
| MS.gene05447 | MS.gene006634 | PPI |
| MS.gene055771 | MS.gene05447 | PPI |
| MS.gene05447 | MS.gene013049 | PPI |
| MS.gene05447 | MS.gene48433 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05447.t1 | MTR_8g442420 | 96.078 | 561 | 22 | 0 | 1 | 561 | 45 | 605 | 0.0 | 1087 |
| MS.gene05447.t1 | MTR_6g084140 | 96.589 | 557 | 19 | 0 | 3 | 559 | 64 | 620 | 0.0 | 1083 |
| MS.gene05447.t1 | MTR_6g084140 | 96.589 | 557 | 19 | 0 | 3 | 559 | 47 | 603 | 0.0 | 1082 |
| MS.gene05447.t1 | MTR_7g105220 | 45.217 | 115 | 55 | 2 | 3 | 117 | 56 | 162 | 1.98e-23 | 97.4 |
| MS.gene05447.t1 | MTR_8g036015 | 39.167 | 120 | 65 | 2 | 3 | 122 | 47 | 158 | 1.93e-19 | 85.9 |
| MS.gene05447.t1 | MTR_5g015500 | 38.211 | 123 | 68 | 2 | 3 | 125 | 351 | 465 | 1.93e-18 | 89.0 |
| MS.gene05447.t1 | MTR_5g015500 | 38.211 | 123 | 68 | 2 | 3 | 125 | 391 | 505 | 2.00e-18 | 89.4 |
| MS.gene05447.t1 | MTR_2g085075 | 40.000 | 115 | 61 | 2 | 3 | 117 | 508 | 614 | 9.93e-18 | 87.0 |
| MS.gene05447.t1 | MTR_2g023120 | 45.161 | 124 | 58 | 5 | 3 | 124 | 59 | 174 | 3.92e-15 | 78.6 |
| MS.gene05447.t1 | MTR_7g095750 | 39.640 | 111 | 56 | 4 | 3 | 112 | 69 | 169 | 8.81e-15 | 77.8 |
| MS.gene05447.t1 | MTR_7g116340 | 37.838 | 111 | 58 | 4 | 4 | 113 | 66 | 166 | 5.32e-14 | 70.9 |
| MS.gene05447.t1 | MTR_3g117750 | 42.727 | 110 | 52 | 5 | 4 | 112 | 74 | 173 | 6.00e-14 | 70.9 |
| MS.gene05447.t1 | MTR_1g117010 | 41.441 | 111 | 55 | 4 | 3 | 112 | 134 | 235 | 2.82e-13 | 70.1 |
| MS.gene05447.t1 | MTR_1g117010 | 41.441 | 111 | 55 | 4 | 3 | 112 | 134 | 235 | 2.82e-13 | 70.1 |
| MS.gene05447.t1 | MTR_4g075290 | 42.202 | 109 | 52 | 6 | 5 | 112 | 67 | 165 | 5.12e-13 | 67.8 |
| MS.gene05447.t1 | MTR_1g085560 | 38.889 | 126 | 58 | 8 | 3 | 123 | 139 | 250 | 9.14e-12 | 65.9 |
| MS.gene05447.t1 | MTR_3g463900 | 40.000 | 110 | 55 | 6 | 4 | 112 | 96 | 195 | 1.52e-11 | 64.3 |
| MS.gene05447.t1 | MTR_4g086760 | 35.000 | 100 | 56 | 2 | 13 | 112 | 77 | 167 | 6.36e-11 | 64.3 |
| MS.gene05447.t1 | MTR_4g086760 | 35.000 | 100 | 56 | 2 | 13 | 112 | 77 | 167 | 6.81e-11 | 64.7 |
| MS.gene05447.t1 | MTR_4g086760 | 35.000 | 100 | 56 | 2 | 13 | 112 | 77 | 167 | 7.14e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05447.t1 | AT1G53720 | 77.961 | 363 | 68 | 4 | 1 | 360 | 45 | 398 | 0.0 | 589 |
| MS.gene05447.t1 | AT1G53720 | 83.618 | 293 | 48 | 0 | 1 | 293 | 45 | 337 | 0.0 | 521 |
| MS.gene05447.t1 | AT1G53780 | 50.222 | 225 | 92 | 3 | 33 | 257 | 3 | 207 | 4.06e-54 | 194 |
| MS.gene05447.t1 | AT1G53780 | 48.058 | 206 | 87 | 3 | 52 | 257 | 1 | 186 | 1.26e-43 | 164 |
| MS.gene05447.t1 | AT1G53780 | 48.058 | 206 | 87 | 3 | 52 | 257 | 1 | 186 | 1.26e-43 | 164 |
| MS.gene05447.t1 | AT1G53780 | 48.058 | 206 | 87 | 3 | 52 | 257 | 1 | 186 | 1.44e-43 | 164 |
| MS.gene05447.t1 | AT2G36130 | 44.915 | 118 | 57 | 3 | 1 | 118 | 54 | 163 | 2.67e-24 | 100 |
| MS.gene05447.t1 | AT1G01940 | 39.167 | 120 | 65 | 2 | 3 | 122 | 47 | 158 | 4.27e-19 | 85.1 |
| MS.gene05447.t1 | AT3G44600 | 40.708 | 113 | 59 | 2 | 5 | 117 | 524 | 628 | 1.13e-17 | 87.0 |
| MS.gene05447.t1 | AT4G33060 | 43.089 | 123 | 59 | 5 | 5 | 124 | 61 | 175 | 1.61e-17 | 86.3 |
| MS.gene05447.t1 | AT5G67530 | 37.500 | 120 | 67 | 2 | 3 | 122 | 390 | 501 | 2.09e-16 | 82.8 |
| MS.gene05447.t1 | AT2G21130 | 40.541 | 111 | 53 | 5 | 4 | 112 | 67 | 166 | 1.12e-14 | 72.8 |
| MS.gene05447.t1 | AT4G38740 | 41.441 | 111 | 52 | 6 | 4 | 112 | 66 | 165 | 2.46e-14 | 71.6 |
| MS.gene05447.t1 | AT5G13120 | 41.964 | 112 | 53 | 5 | 3 | 112 | 147 | 248 | 9.22e-14 | 72.0 |
| MS.gene05447.t1 | AT5G13120 | 41.964 | 112 | 53 | 5 | 3 | 112 | 143 | 244 | 1.04e-13 | 71.6 |
| MS.gene05447.t1 | AT3G63400 | 36.937 | 111 | 59 | 4 | 3 | 112 | 69 | 169 | 2.89e-13 | 72.8 |
| MS.gene05447.t1 | AT3G63400 | 36.937 | 111 | 59 | 4 | 3 | 112 | 69 | 169 | 2.89e-13 | 72.8 |
| MS.gene05447.t1 | AT3G63400 | 36.937 | 111 | 59 | 4 | 3 | 112 | 69 | 169 | 2.89e-13 | 72.8 |
| MS.gene05447.t1 | AT3G63400 | 36.937 | 111 | 59 | 4 | 3 | 112 | 69 | 169 | 3.13e-13 | 72.0 |
| MS.gene05447.t1 | AT3G56070 | 37.288 | 118 | 63 | 5 | 5 | 121 | 67 | 174 | 3.22e-13 | 68.6 |
| MS.gene05447.t1 | AT3G56070 | 37.288 | 118 | 63 | 5 | 5 | 121 | 67 | 174 | 3.22e-13 | 68.6 |
| MS.gene05447.t1 | AT2G16600 | 42.202 | 109 | 52 | 5 | 5 | 112 | 68 | 166 | 7.03e-13 | 67.4 |
| MS.gene05447.t1 | AT3G26420 | 27.917 | 240 | 125 | 6 | 197 | 404 | 5 | 228 | 3.60e-12 | 67.0 |
| MS.gene05447.t1 | AT2G16600 | 41.284 | 109 | 48 | 5 | 4 | 112 | 52 | 144 | 4.46e-12 | 64.7 |
| MS.gene05447.t1 | AT2G15790 | 35.135 | 111 | 62 | 3 | 3 | 112 | 66 | 167 | 1.78e-11 | 66.2 |
Find 134 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATACATGCGGAAAGATAATA+AGG | 0.255757 | 6.4:+58103657 | MS.gene05447:CDS |
| TGGTGTTTCGTTTCTCTCTT+TGG | 0.263367 | 6.4:-58104061 | None:intergenic |
| GAAAGACAGAGAAAGGAATA+TGG | 0.266494 | 6.4:+58104291 | MS.gene05447:CDS |
| TTTCCTTCTGGTGAAGCTTC+AGG | 0.292417 | 6.4:-58099922 | None:intergenic |
| CCTTTCTCTGTCTTTCTTGT+CGG | 0.295130 | 6.4:-58104284 | None:intergenic |
| CGTTTGTAATCATCGCCATC+TGG | 0.307386 | 6.4:-58104535 | None:intergenic |
| GATCAAAGGAAGCACATTCT+AGG | 0.318080 | 6.4:+58100979 | MS.gene05447:CDS |
| GAATCCTATTCCCATGAATA+AGG | 0.321604 | 6.4:+58095465 | None:intergenic |
| TGCTTTATCTCCACCCCGTT+GGG | 0.336670 | 6.4:-58103914 | None:intergenic |
| AGTTTATGCTATGCTTTCAT+AGG | 0.347181 | 6.4:+58101483 | MS.gene05447:CDS |
| CAAGATGTGAAGGTTGATTC+TGG | 0.347476 | 6.4:+58104496 | MS.gene05447:CDS |
| CATGTATTGTGACCATAGTT+TGG | 0.349002 | 6.4:-58103641 | None:intergenic |
| GGAGGGATGACCGAGATTAT+AGG | 0.351226 | 6.4:+58104449 | MS.gene05447:CDS |
| GTCAAAGTGTTGCCAAACTA+TGG | 0.365707 | 6.4:+58103629 | MS.gene05447:CDS |
| GCTCTCTGTTACCTTATTCA+TGG | 0.391179 | 6.4:-58095476 | None:intergenic |
| TTCTTGTCGGCATGAAAATC+CGG | 0.400986 | 6.4:-58104271 | None:intergenic |
| TGGGTTTAATTGTTCATCCA+TGG | 0.402075 | 6.4:-58100934 | None:intergenic |
| GATGACCTTGATTACCTTGA+CGG | 0.406436 | 6.4:+58096474 | MS.gene05447:CDS |
| GAAGATGTGAAGATTGATTC+CGG | 0.425382 | 6.4:+58104583 | MS.gene05447:CDS |
| GAAGGACAATAATGCCCAAC+GGG | 0.426794 | 6.4:+58103900 | MS.gene05447:CDS |
| GACAGAGTTCGAAGACAAAC+TGG | 0.432726 | 6.4:+58101841 | MS.gene05447:intron |
| TTCATGGTGTAGATACGAAA+TGG | 0.436440 | 6.4:+58104012 | MS.gene05447:intron |
| GGCGGTTTAACCTCAGCATC+TGG | 0.439744 | 6.4:-58101122 | None:intergenic |
| TCATAGAGGTCGTAGGGATC+AGG | 0.444751 | 6.4:+58104147 | MS.gene05447:CDS |
| GGGAAGGTCGTGAACATAGA+AGG | 0.446283 | 6.4:+58104677 | MS.gene05447:CDS |
| GGGTTTAATTGTTCATCCAT+GGG | 0.447168 | 6.4:-58100933 | None:intergenic |
| ATAACCTCTTCAAGTTCTCC+TGG | 0.449225 | 6.4:-58100954 | None:intergenic |
| GAAAACGAAGCCTGGATGAT+GGG | 0.454580 | 6.4:+58104611 | MS.gene05447:CDS |
| CCTACTGGAACTGGCACTGC+TGG | 0.458406 | 6.4:+58095784 | MS.gene05447:CDS |
| GATCATCAAAAGGATCTTCC+AGG | 0.462705 | 6.4:-58099876 | None:intergenic |
| AGATTACCCACCTCAGTAAC+CGG | 0.469687 | 6.4:-58101176 | None:intergenic |
| ACTGGTGATCCTACTGGAAC+TGG | 0.474821 | 6.4:+58095775 | MS.gene05447:CDS |
| CAGTTCTCTTCCTATAATCT+CGG | 0.479487 | 6.4:-58104459 | None:intergenic |
| AAAGACAGAGAAAGGAATAT+GGG | 0.483710 | 6.4:+58104292 | MS.gene05447:CDS |
| AGCAGAGGGCTTGAAGGAAA+CGG | 0.483995 | 6.4:+58104211 | MS.gene05447:CDS |
| GGAAAGAGGTGCAAGAGATC+CGG | 0.484681 | 6.4:+58104252 | MS.gene05447:CDS |
| GATCTAAAGCATTCTAAGAC+TGG | 0.487196 | 6.4:+58096239 | MS.gene05447:CDS |
| AGAGAACTGAAGACAGTGTA+AGG | 0.488015 | 6.4:+58104473 | MS.gene05447:CDS |
| AGAAAACGAAGCCTGGATGA+TGG | 0.488745 | 6.4:+58104610 | MS.gene05447:CDS |
| GAGGCTTATGCCGATGGAAA+GGG | 0.489014 | 6.4:+58099708 | MS.gene05447:CDS |
| CTCTCTGTTACCTTATTCAT+GGG | 0.489784 | 6.4:-58095475 | None:intergenic |
| GCGATGATTACAAACGTAGA+AGG | 0.490035 | 6.4:+58104542 | MS.gene05447:CDS |
| CGGAGATGATAAAGCGGGAC+TGG | 0.492772 | 6.4:+58104231 | MS.gene05447:CDS |
| TTGCTTTATCTCCACCCCGT+TGG | 0.493840 | 6.4:-58103915 | None:intergenic |
| GACGGGATAGTCGAGATGAT+AGG | 0.494353 | 6.4:+58104398 | MS.gene05447:CDS |
| TCCTATGTCTGTAATCCTCA+TGG | 0.495780 | 6.4:-58104654 | None:intergenic |
| GACTTTGACTAGAATTAATG+AGG | 0.499789 | 6.4:+58099689 | MS.gene05447:CDS |
| GATAGAAGCAGAGGGCTTGA+AGG | 0.500538 | 6.4:+58104205 | MS.gene05447:CDS |
| GGTCATCCCTTTCATATCGC+TGG | 0.507902 | 6.4:-58104081 | None:intergenic |
| AGAGAATTTGAATGCTTCAC+AGG | 0.509447 | 6.4:+58096286 | MS.gene05447:CDS |
| CGGACGAAGAAAACGAAGCC+TGG | 0.509886 | 6.4:+58104603 | MS.gene05447:CDS |
| TGAAGAGGTTATTAGATCAA+AGG | 0.514059 | 6.4:+58100965 | MS.gene05447:CDS |
| AAGTCCGACTTGAAGATGAT+TGG | 0.517976 | 6.4:+58100907 | MS.gene05447:CDS |
| GGTCATCCCTCCTATCCAAA+TGG | 0.522011 | 6.4:-58104438 | None:intergenic |
| TGAGGCTTATGCCGATGGAA+AGG | 0.522232 | 6.4:+58099707 | MS.gene05447:CDS |
| CCGACAAGAAAGACAGAGAA+AGG | 0.522500 | 6.4:+58104284 | MS.gene05447:CDS |
| AAATGGTCTTCCCTTTCCAT+CGG | 0.529485 | 6.4:-58099718 | None:intergenic |
| AAGGATTTCACTGCTCAGAC+TGG | 0.532033 | 6.4:+58095757 | MS.gene05447:intron |
| AGACAAACTGGCATGTGAGC+AGG | 0.532699 | 6.4:+58101853 | MS.gene05447:CDS |
| GTTCCACTGATCATATTGCT+AGG | 0.534319 | 6.4:+58103830 | MS.gene05447:CDS |
| ATCAAAGGAAGCACATTCTA+GGG | 0.534373 | 6.4:+58100980 | MS.gene05447:CDS |
| TAACCTCTTCAAGTTCTCCT+GGG | 0.534396 | 6.4:-58100953 | None:intergenic |
| GTTTGTAATCCCCATCATCC+AGG | 0.535057 | 6.4:-58104621 | None:intergenic |
| ATGACAGCCATTTGGATAGG+AGG | 0.535172 | 6.4:+58104431 | MS.gene05447:CDS |
| AGTTGAGGAGGATCATCAAA+AGG | 0.535842 | 6.4:-58099886 | None:intergenic |
| ATGATAGTCATAGAGGTCGT+AGG | 0.538687 | 6.4:+58104140 | MS.gene05447:CDS |
| GCTTTCCGTCAAGGTAATCA+AGG | 0.538704 | 6.4:-58096479 | None:intergenic |
| GCTCAGACTGGTGATCCTAC+TGG | 0.539336 | 6.4:+58095769 | MS.gene05447:CDS |
| ATCATTCGTGATCACAAGAC+TGG | 0.543603 | 6.4:+58101456 | MS.gene05447:CDS |
| ACCCACAAAATTTATCATGA+AGG | 0.545661 | 6.4:+58103882 | MS.gene05447:CDS |
| TTACTGTATGCTTTCCGTCA+AGG | 0.548412 | 6.4:-58096488 | None:intergenic |
| TTTAAAGATGATAGTCATAG+AGG | 0.548618 | 6.4:+58104133 | MS.gene05447:CDS |
| AGGTCGTAGGGATCAGGAGA+TGG | 0.548677 | 6.4:+58104153 | MS.gene05447:CDS |
| ATGCTTTGATTGATGATCGA+AGG | 0.550568 | 6.4:+58103590 | MS.gene05447:CDS |
| ATTCCTGAAGCTTCACCAGA+AGG | 0.553060 | 6.4:+58099919 | MS.gene05447:CDS |
| ATTAATGAGGCTTATGCCGA+TGG | 0.553811 | 6.4:+58099702 | MS.gene05447:CDS |
| GACAATAATGCCCAACGGGG+TGG | 0.555935 | 6.4:+58103904 | MS.gene05447:CDS |
| TGAAGATGATTGGGTGCCCA+TGG | 0.556542 | 6.4:+58100917 | MS.gene05447:CDS |
| AGAAGAAAACGAAGCCCAGA+TGG | 0.556598 | 6.4:+58104520 | MS.gene05447:CDS |
| CGAAACACCAGCGATATGAA+AGG | 0.557586 | 6.4:+58104074 | MS.gene05447:CDS |
| GCAGGCTCGAATAACAGAGA+TGG | 0.559927 | 6.4:+58104175 | MS.gene05447:CDS |
| AATCAAGCACACTTACATCC+TGG | 0.562265 | 6.4:+58099858 | MS.gene05447:CDS |
| TTCCACTGATCATATTGCTA+GGG | 0.563783 | 6.4:+58103831 | MS.gene05447:CDS |
| CAGTGTAAGGCAAGATGTGA+AGG | 0.563971 | 6.4:+58104486 | MS.gene05447:CDS |
| TGACAGCCATTTGGATAGGA+GGG | 0.564950 | 6.4:+58104432 | MS.gene05447:CDS |
| GAAACACCAGCGATATGAAA+GGG | 0.564974 | 6.4:+58104075 | MS.gene05447:CDS |
| CGTAGGGATCAGGAGATGGC+AGG | 0.568227 | 6.4:+58104157 | MS.gene05447:CDS |
| TGATAGTCATAGAGGTCGTA+GGG | 0.569876 | 6.4:+58104141 | MS.gene05447:CDS |
| ATTCTCTCCTGCACTAGCCA+TGG | 0.569975 | 6.4:-58096270 | None:intergenic |
| AGTCCGACTTGAAGATGATT+GGG | 0.570247 | 6.4:+58100908 | MS.gene05447:CDS |
| CATATTGCTAGGGATTGCAC+TGG | 0.570802 | 6.4:+58103841 | MS.gene05447:CDS |
| GTTTGTAATCATCGCCATCT+GGG | 0.573839 | 6.4:-58104534 | None:intergenic |
| GATAAAGCGGGACTGGAAAG+AGG | 0.576342 | 6.4:+58104238 | MS.gene05447:CDS |
| TACATGCGGAAAGATAATAA+GGG | 0.576638 | 6.4:+58103658 | MS.gene05447:CDS |
| ATGATAAATACACAGAAAGA+CGG | 0.577626 | 6.4:+58104380 | MS.gene05447:CDS |
| AGATGCTGAGGTTAAACCGC+CGG | 0.579812 | 6.4:+58101124 | MS.gene05447:CDS |
| GGAAACGGAGATGATAAAGC+GGG | 0.583781 | 6.4:+58104226 | MS.gene05447:CDS |
| TGATAAATACACAGAAAGAC+GGG | 0.584231 | 6.4:+58104381 | MS.gene05447:CDS |
| CAGGCTCGAATAACAGAGAT+GGG | 0.587573 | 6.4:+58104176 | MS.gene05447:CDS |
| CAGATGACAGCCATTTGGAT+AGG | 0.587905 | 6.4:+58104428 | MS.gene05447:CDS |
| AGCGATATGAAAGGGATGAC+CGG | 0.593576 | 6.4:+58104083 | MS.gene05447:CDS |
| ATCCCTAGCAATATGATCAG+TGG | 0.596232 | 6.4:-58103833 | None:intergenic |
| AGGAAACGGAGATGATAAAG+CGG | 0.596287 | 6.4:+58104225 | MS.gene05447:CDS |
| ATGGGGATTACAAACGTGGA+AGG | 0.597862 | 6.4:+58104629 | MS.gene05447:CDS |
| AGTCTAGCTCATCTTTCCGT+CGG | 0.602521 | 6.4:-58104339 | None:intergenic |
| AAAACGAAGCCTGGATGATG+GGG | 0.602978 | 6.4:+58104612 | MS.gene05447:CDS |
| ATGAGGATTACAGACATAGG+AGG | 0.605188 | 6.4:+58104656 | MS.gene05447:CDS |
| ACTGTTGCCATGGCTAGTGC+AGG | 0.608593 | 6.4:+58096263 | MS.gene05447:CDS |
| TGGGGATATTCCAGATGCTG+AGG | 0.610469 | 6.4:+58101112 | MS.gene05447:CDS |
| TAAGACTGGAACTGTTGCCA+TGG | 0.613040 | 6.4:+58096253 | MS.gene05447:CDS |
| AACTATGGTCACAATACATG+CGG | 0.620215 | 6.4:+58103644 | MS.gene05447:CDS |
| GATTACAGACATAGGAGGGA+AGG | 0.622385 | 6.4:+58104661 | MS.gene05447:CDS |
| GGTGGTGGTTGCTTCAAATG+TGG | 0.622769 | 6.4:+58103808 | MS.gene05447:intron |
| ATGAACTCAGATAGTTGAGG+AGG | 0.626962 | 6.4:-58099898 | None:intergenic |
| AAACCCAGGAGAACTTGAAG+AGG | 0.634019 | 6.4:+58100950 | MS.gene05447:CDS |
| TGGGGATTACAAACGTGGAA+GGG | 0.636249 | 6.4:+58104630 | MS.gene05447:CDS |
| AACGGGGTGGAGATAAAGCA+AGG | 0.636526 | 6.4:+58103917 | MS.gene05447:CDS |
| GGGTATCGCGATAGAAGCAG+AGG | 0.641203 | 6.4:+58104196 | MS.gene05447:CDS |
| CCAGCAGTGCCAGTTCCAGT+AGG | 0.645974 | 6.4:-58095784 | None:intergenic |
| TAAATTGAATCCGGTTACTG+AGG | 0.648422 | 6.4:+58101166 | MS.gene05447:CDS |
| TGAAGGACAATAATGCCCAA+CGG | 0.661625 | 6.4:+58103899 | MS.gene05447:CDS |
| TGAGGATTACAGACATAGGA+GGG | 0.662466 | 6.4:+58104657 | MS.gene05447:CDS |
| ACCATGAGGATTACAGACAT+AGG | 0.668184 | 6.4:+58104653 | MS.gene05447:CDS |
| GCACCCAATCATCTTCAAGT+CGG | 0.669949 | 6.4:-58100911 | None:intergenic |
| CAAACGTAGAAGGAATGATG+AGG | 0.682353 | 6.4:+58104552 | MS.gene05447:CDS |
| ATGGATGAACAATTAAACCC+AGG | 0.685321 | 6.4:+58100936 | MS.gene05447:CDS |
| GATGATGGGGATTACAAACG+TGG | 0.687670 | 6.4:+58104625 | MS.gene05447:CDS |
| TGATGATCGAAGGATACATG+TGG | 0.693355 | 6.4:+58103600 | MS.gene05447:CDS |
| GGAATGAACTCAGATAGTTG+AGG | 0.698164 | 6.4:-58099901 | None:intergenic |
| GGTATCGCGATAGAAGCAGA+GGG | 0.701621 | 6.4:+58104197 | MS.gene05447:CDS |
| ATAGAGACGATGACTACCGA+CGG | 0.712917 | 6.4:+58104323 | MS.gene05447:CDS |
| AGAAGGAAAACCTAAAGACG+AGG | 0.723882 | 6.4:+58099936 | MS.gene05447:CDS |
| CAAACGTGGAAGGGACCATG+AGG | 0.747318 | 6.4:+58104639 | MS.gene05447:CDS |
| AAGGACAATAATGCCCAACG+GGG | 0.775217 | 6.4:+58103901 | MS.gene05447:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 58095478 | 58104705 | 58095478 | ID=MS.gene05447 |
| chr6.4 | mRNA | 58095478 | 58104705 | 58095478 | ID=MS.gene05447.t1;Parent=MS.gene05447 |
| chr6.4 | exon | 58095478 | 58095486 | 58095478 | ID=MS.gene05447.t1.exon1;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58095478 | 58095486 | 58095478 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58095760 | 58095821 | 58095760 | ID=MS.gene05447.t1.exon2;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58095760 | 58095821 | 58095760 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58096190 | 58096307 | 58096190 | ID=MS.gene05447.t1.exon3;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58096190 | 58096307 | 58096190 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58096456 | 58096506 | 58096456 | ID=MS.gene05447.t1.exon4;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58096456 | 58096506 | 58096456 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58099660 | 58099751 | 58099660 | ID=MS.gene05447.t1.exon5;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58099660 | 58099751 | 58099660 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58099858 | 58099957 | 58099858 | ID=MS.gene05447.t1.exon6;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58099858 | 58099957 | 58099858 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58100897 | 58101019 | 58100897 | ID=MS.gene05447.t1.exon7;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58100897 | 58101019 | 58100897 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58101110 | 58101187 | 58101110 | ID=MS.gene05447.t1.exon8;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58101110 | 58101187 | 58101110 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58101308 | 58101354 | 58101308 | ID=MS.gene05447.t1.exon9;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58101308 | 58101354 | 58101308 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58101449 | 58101504 | 58101449 | ID=MS.gene05447.t1.exon10;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58101449 | 58101504 | 58101449 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58101846 | 58101886 | 58101846 | ID=MS.gene05447.t1.exon11;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58101846 | 58101886 | 58101846 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58103583 | 58103679 | 58103583 | ID=MS.gene05447.t1.exon12;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58103583 | 58103679 | 58103583 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58103809 | 58103938 | 58103809 | ID=MS.gene05447.t1.exon13;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58103809 | 58103938 | 58103809 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| chr6.4 | exon | 58104024 | 58104705 | 58104024 | ID=MS.gene05447.t1.exon14;Parent=MS.gene05447.t1 |
| chr6.4 | CDS | 58104024 | 58104705 | 58104024 | ID=cds.MS.gene05447.t1;Parent=MS.gene05447.t1 |
| Gene Sequence |
| Protein sequence |
