Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58434.t1 | XP_013441833.1 | 97.2 | 218 | 6 | 0 | 1 | 218 | 117 | 334 | 1.10E-112 | 416 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58434.t1 | P81392 | 38.2 | 191 | 77 | 9 | 23 | 197 | 142 | 307 | 2.3e-10 | 67.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58434.t1 | A0A072TFY2 | 97.2 | 218 | 6 | 0 | 1 | 218 | 117 | 334 | 7.8e-113 | 416.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene58434 | 0.816044 | 6.77E-52 | -1.69E-46 |
| MS.gene049369 | MS.gene58434 | 0.816045 | 6.77E-52 | -1.69E-46 |
| MS.gene049396 | MS.gene58434 | 0.836043 | 1.18E-56 | -1.69E-46 |
| MS.gene050499 | MS.gene58434 | 0.818559 | 1.84E-52 | -1.69E-46 |
| MS.gene050502 | MS.gene58434 | 0.817343 | 3.46E-52 | -1.69E-46 |
| MS.gene050593 | MS.gene58434 | 0.809838 | 1.55E-50 | -1.69E-46 |
| MS.gene050670 | MS.gene58434 | 0.815449 | 9.19E-52 | -1.69E-46 |
| MS.gene050686 | MS.gene58434 | 0.813542 | 2.43E-51 | -1.69E-46 |
| MS.gene050971 | MS.gene58434 | 0.825784 | 3.88E-54 | -1.69E-46 |
| MS.gene051387 | MS.gene58434 | 0.823005 | 1.75E-53 | -1.69E-46 |
| MS.gene052346 | MS.gene58434 | 0.820155 | 7.97E-53 | -1.69E-46 |
| MS.gene052414 | MS.gene58434 | 0.810112 | 1.36E-50 | -1.69E-46 |
| MS.gene052999 | MS.gene58434 | 0.811146 | 8.10E-51 | -1.69E-46 |
| MS.gene053169 | MS.gene58434 | 0.823606 | 1.27E-53 | -1.69E-46 |
| MS.gene053449 | MS.gene58434 | 0.850994 | 1.18E-60 | -1.69E-46 |
| MS.gene053505 | MS.gene58434 | 0.837483 | 5.06E-57 | -1.69E-46 |
| MS.gene053758 | MS.gene58434 | 0.821522 | 3.86E-53 | -1.69E-46 |
| MS.gene053813 | MS.gene58434 | 0.845286 | 4.46E-59 | -1.69E-46 |
| MS.gene053912 | MS.gene58434 | 0.809663 | 1.69E-50 | -1.69E-46 |
| MS.gene054486 | MS.gene58434 | 0.853579 | 2.18E-61 | -1.69E-46 |
| MS.gene054556 | MS.gene58434 | 0.80232 | 5.93E-49 | -1.69E-46 |
| MS.gene05505 | MS.gene58434 | 0.804998 | 1.65E-49 | -1.69E-46 |
| MS.gene055909 | MS.gene58434 | 0.861009 | 1.39E-63 | -1.69E-46 |
| MS.gene055911 | MS.gene58434 | 0.864657 | 1.04E-64 | -1.69E-46 |
| MS.gene05601 | MS.gene58434 | 0.800571 | 1.35E-48 | -1.69E-46 |
| MS.gene056422 | MS.gene58434 | 0.851304 | 9.68E-61 | -1.69E-46 |
| MS.gene056448 | MS.gene58434 | 0.808285 | 3.34E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene58434 | 0.856879 | 2.39E-62 | -1.69E-46 |
| MS.gene057019 | MS.gene58434 | 0.804951 | 1.69E-49 | -1.69E-46 |
| MS.gene057086 | MS.gene58434 | 0.816144 | 6.43E-52 | -1.69E-46 |
| MS.gene057734 | MS.gene58434 | 0.816959 | 4.23E-52 | -1.69E-46 |
| MS.gene057823 | MS.gene58434 | 0.814462 | 1.52E-51 | -1.69E-46 |
| MS.gene058007 | MS.gene58434 | 0.810214 | 1.29E-50 | -1.69E-46 |
| MS.gene058075 | MS.gene58434 | 0.81182 | 5.78E-51 | -1.69E-46 |
| MS.gene058700 | MS.gene58434 | 0.808854 | 2.52E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene58434 | 0.807385 | 5.19E-50 | -1.69E-46 |
| MS.gene058928 | MS.gene58434 | 0.811844 | 5.71E-51 | -1.69E-46 |
| MS.gene058995 | MS.gene58434 | 0.83292 | 7.18E-56 | -1.69E-46 |
| MS.gene059366 | MS.gene58434 | 0.816837 | 4.50E-52 | -1.69E-46 |
| MS.gene059368 | MS.gene58434 | 0.837577 | 4.79E-57 | -1.69E-46 |
| MS.gene059674 | MS.gene58434 | 0.841488 | 4.61E-58 | -1.69E-46 |
| MS.gene059711 | MS.gene58434 | 0.837416 | 5.26E-57 | -1.69E-46 |
| MS.gene059807 | MS.gene58434 | 0.853362 | 2.51E-61 | -1.69E-46 |
| MS.gene059933 | MS.gene58434 | 0.804932 | 1.70E-49 | -1.69E-46 |
| MS.gene060372 | MS.gene58434 | 0.814553 | 1.45E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene58434 | 0.82363 | 1.25E-53 | -1.69E-46 |
| MS.gene060482 | MS.gene58434 | 0.804409 | 2.19E-49 | -1.69E-46 |
| MS.gene060835 | MS.gene58434 | 0.816997 | 4.14E-52 | -1.69E-46 |
| MS.gene060837 | MS.gene58434 | 0.861626 | 8.99E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58434.t1 | MTR_0140s0030 | 97.248 | 218 | 6 | 0 | 1 | 218 | 117 | 334 | 8.20e-155 | 432 |
| MS.gene58434.t1 | MTR_0489s0020 | 97.248 | 218 | 6 | 0 | 1 | 218 | 117 | 334 | 8.20e-155 | 432 |
| MS.gene58434.t1 | MTR_3g039990 | 38.222 | 225 | 95 | 9 | 4 | 218 | 121 | 311 | 1.27e-29 | 112 |
| MS.gene58434.t1 | MTR_6g012180 | 35.533 | 197 | 108 | 7 | 11 | 205 | 130 | 309 | 2.71e-20 | 87.4 |
| MS.gene58434.t1 | MTR_7g010210 | 31.718 | 227 | 107 | 10 | 1 | 206 | 116 | 315 | 3.71e-16 | 76.3 |
| MS.gene58434.t1 | MTR_7g010210 | 40.678 | 118 | 43 | 4 | 1 | 105 | 116 | 219 | 9.07e-14 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58434.t1 | AT1G74650 | 33.503 | 197 | 98 | 7 | 23 | 211 | 75 | 246 | 3.22e-16 | 75.5 |
| MS.gene58434.t1 | AT1G74650 | 33.503 | 197 | 98 | 7 | 23 | 211 | 80 | 251 | 5.18e-16 | 74.7 |
| MS.gene58434.t1 | AT1G74650 | 33.503 | 197 | 98 | 7 | 23 | 211 | 156 | 327 | 3.27e-15 | 73.6 |
| MS.gene58434.t1 | AT3G47600 | 35.176 | 199 | 97 | 10 | 23 | 212 | 147 | 322 | 5.60e-15 | 72.8 |
| MS.gene58434.t1 | AT3G47600 | 35.176 | 199 | 97 | 10 | 23 | 212 | 153 | 328 | 6.95e-15 | 72.8 |
| MS.gene58434.t1 | AT3G28910 | 30.366 | 191 | 87 | 5 | 23 | 197 | 73 | 233 | 7.50e-14 | 68.9 |
| MS.gene58434.t1 | AT3G28910 | 30.366 | 191 | 87 | 5 | 23 | 197 | 149 | 309 | 2.13e-13 | 68.2 |
| MS.gene58434.t1 | AT5G62470 | 32.751 | 229 | 98 | 11 | 23 | 217 | 146 | 352 | 2.42e-13 | 68.2 |
| MS.gene58434.t1 | AT5G62470 | 32.751 | 229 | 98 | 11 | 23 | 217 | 145 | 351 | 3.09e-13 | 68.2 |
Find 45 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGATTATGCATCTCGATTT+AGG | 0.240478 | 8.3:+15969057 | MS.gene58434:CDS |
| GATTAATATTAAAGCAAATA+TGG | 0.292292 | 8.3:+15969151 | MS.gene58434:CDS |
| TGAAAACATTTCTAGATTGA+TGG | 0.297412 | 8.3:+15968899 | MS.gene58434:CDS |
| AGCCTCAGATAAGGCTTGTT+TGG | 0.361155 | 8.3:-15968748 | None:intergenic |
| CTTTCCCATTGACCTTTATA+CGG | 0.370223 | 8.3:-15968699 | None:intergenic |
| TCGAGATGCATAATCAGATT+TGG | 0.373847 | 8.3:-15969051 | None:intergenic |
| CGTTCGCAGCTGCCGTATAA+AGG | 0.376269 | 8.3:+15968687 | MS.gene58434:CDS |
| TTTGTTTCAGCTGATGAGTT+AGG | 0.376364 | 8.3:-15968939 | None:intergenic |
| ACATGTTTATAAGCTCTTCA+TGG | 0.378049 | 8.3:-15969241 | None:intergenic |
| CATATGGAGATGGAATATTC+AGG | 0.382987 | 8.3:-15968866 | None:intergenic |
| GCTTATAAACATGTCATTAG+AGG | 0.404105 | 8.3:+15969250 | MS.gene58434:CDS |
| AATCAGATTTGGTTGAGTTA+AGG | 0.409124 | 8.3:-15969040 | None:intergenic |
| GATGAGGGTTTCATCTGATC+AGG | 0.419961 | 8.3:-15968810 | None:intergenic |
| TAATATTAATCCTTTCCTCT+TGG | 0.421047 | 8.3:-15969139 | None:intergenic |
| GGAGATGGAATATTCAGGTT+TGG | 0.447433 | 8.3:-15968861 | None:intergenic |
| TATTAATCCTTTCCTCTTGG+TGG | 0.453369 | 8.3:-15969136 | None:intergenic |
| AGCTGCCGTATAAAGGTCAA+TGG | 0.472504 | 8.3:+15968694 | MS.gene58434:CDS |
| ATCGATATTCAGCAACATGC+AGG | 0.473325 | 8.3:+15968968 | MS.gene58434:CDS |
| TTTCTAGATTGATGGAAACT+TGG | 0.473758 | 8.3:+15968907 | MS.gene58434:CDS |
| GGCAAAGTACCAAGGGTTGT+AGG | 0.474388 | 8.3:-15968789 | None:intergenic |
| GGGTTGTAGGGTTATGTTGA+AGG | 0.484469 | 8.3:-15968776 | None:intergenic |
| GATGGAATATTCAGGTTTGG+TGG | 0.513419 | 8.3:-15968858 | None:intergenic |
| TATTAAAGCAAATATGGAAA+CGG | 0.514444 | 8.3:+15969157 | MS.gene58434:CDS |
| GCTACAAACAGATATTCATA+TGG | 0.516194 | 8.3:+15968725 | MS.gene58434:CDS |
| CTTCAACTTGAACACAAAAG+AGG | 0.519329 | 8.3:+15969103 | MS.gene58434:CDS |
| CAACATAACCCTACAACCCT+TGG | 0.522290 | 8.3:+15968780 | MS.gene58434:CDS |
| GCAAAGTACCAAGGGTTGTA+GGG | 0.526670 | 8.3:-15968788 | None:intergenic |
| ATAAAGGTCAATGGGAAAGA+AGG | 0.529786 | 8.3:+15968703 | MS.gene58434:CDS |
| TCTTCTTCCACCAAGAGGAA+AGG | 0.547540 | 8.3:+15969129 | MS.gene58434:CDS |
| TAGCTTGATGCATATGGAGA+TGG | 0.555943 | 8.3:-15968876 | None:intergenic |
| GCTGCCGTATAAAGGTCAAT+GGG | 0.556660 | 8.3:+15968695 | MS.gene58434:CDS |
| GATCAAAGTAGTAACGATGA+AGG | 0.558829 | 8.3:+15968648 | MS.gene58434:CDS |
| CGATTTAGGTCAACTCATGA+AGG | 0.562437 | 8.3:+15969071 | MS.gene58434:CDS |
| TTCAACTTGAACACAAAAGA+GGG | 0.572702 | 8.3:+15969104 | MS.gene58434:CDS |
| GGTTGTAGGGTTATGTTGAA+GGG | 0.574998 | 8.3:-15968775 | None:intergenic |
| AAGGGACAAAGCCTCAGATA+AGG | 0.576279 | 8.3:-15968757 | None:intergenic |
| CTTATAAACATGTCATTAGA+GGG | 0.583066 | 8.3:+15969251 | MS.gene58434:CDS |
| CAGGGATCAAGTTGTAGTGA+AGG | 0.587215 | 8.3:+15968987 | MS.gene58434:CDS |
| TCGATATTCAGCAACATGCA+GGG | 0.617549 | 8.3:+15968969 | MS.gene58434:CDS |
| CTGATCAGGCAAAGTACCAA+GGG | 0.619842 | 8.3:-15968796 | None:intergenic |
| TCTGATCAGGCAAAGTACCA+AGG | 0.632255 | 8.3:-15968797 | None:intergenic |
| AGCACAACACAAGATCATCA+TGG | 0.642061 | 8.3:+15969017 | MS.gene58434:CDS |
| GGGTCTCTTCTTCCACCAAG+AGG | 0.658408 | 8.3:+15969124 | MS.gene58434:CDS |
| GGCCAAACAAGCCTTATCTG+AGG | 0.663946 | 8.3:+15968746 | MS.gene58434:CDS |
| CATGTTGCTGAATATCGATG+AGG | 0.741627 | 8.3:-15968964 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTAATATTAAAGCAAATA+TGG | + | chr8.3:15969151-15969170 | MS.gene58434:CDS | 15.0% |
| !! | TATTAAAGCAAATATGGAAA+CGG | + | chr8.3:15969157-15969176 | MS.gene58434:CDS | 20.0% |
| ! | CTTATAAACATGTCATTAGA+GGG | + | chr8.3:15969251-15969270 | MS.gene58434:CDS | 25.0% |
| ! | TAATATTAATCCTTTCCTCT+TGG | - | chr8.3:15969142-15969161 | None:intergenic | 25.0% |
| !! | TGAAAACATTTCTAGATTGA+TGG | + | chr8.3:15968899-15968918 | MS.gene58434:CDS | 25.0% |
| !!! | AAAAATGGCTTTTTGAAGAT+GGG | + | chr8.3:15969201-15969220 | MS.gene58434:CDS | 25.0% |
| ACATGTTTATAAGCTCTTCA+TGG | - | chr8.3:15969244-15969263 | None:intergenic | 30.0% | |
| AGAAAGAGAAAAACTTGATG+AGG | - | chr8.3:15968829-15968848 | None:intergenic | 30.0% | |
| CTCTTACTTTGCTTGAAAAA+TGG | + | chr8.3:15969186-15969205 | MS.gene58434:CDS | 30.0% | |
| GAAAGAGAAAAACTTGATGA+GGG | - | chr8.3:15968828-15968847 | None:intergenic | 30.0% | |
| GCTACAAACAGATATTCATA+TGG | + | chr8.3:15968725-15968744 | MS.gene58434:CDS | 30.0% | |
| GCTTATAAACATGTCATTAG+AGG | + | chr8.3:15969250-15969269 | MS.gene58434:CDS | 30.0% | |
| TTCAACTTGAACACAAAAGA+GGG | + | chr8.3:15969104-15969123 | MS.gene58434:CDS | 30.0% | |
| TTTCTAGATTGATGGAAACT+TGG | + | chr8.3:15968907-15968926 | MS.gene58434:CDS | 30.0% | |
| ! | AATCAGATTTGGTTGAGTTA+AGG | - | chr8.3:15969043-15969062 | None:intergenic | 30.0% |
| ! | TTTTCATAGCTTGATGCATA+TGG | - | chr8.3:15968885-15968904 | None:intergenic | 30.0% |
| !! | AAAATGGCTTTTTGAAGATG+GGG | + | chr8.3:15969202-15969221 | MS.gene58434:CDS | 30.0% |
| !! | GAAAAATGGCTTTTTGAAGA+TGG | + | chr8.3:15969200-15969219 | MS.gene58434:CDS | 30.0% |
| !!! | CATTTTTCAAGCAAAGTAAG+AGG | - | chr8.3:15969188-15969207 | None:intergenic | 30.0% |
| ATAAAGGTCAATGGGAAAGA+AGG | + | chr8.3:15968703-15968722 | MS.gene58434:CDS | 35.0% | |
| CATATGGAGATGGAATATTC+AGG | - | chr8.3:15968869-15968888 | None:intergenic | 35.0% | |
| CTGATTATGCATCTCGATTT+AGG | + | chr8.3:15969057-15969076 | MS.gene58434:CDS | 35.0% | |
| CTTCAACTTGAACACAAAAG+AGG | + | chr8.3:15969103-15969122 | MS.gene58434:CDS | 35.0% | |
| CTTTCCCATTGACCTTTATA+CGG | - | chr8.3:15968702-15968721 | None:intergenic | 35.0% | |
| GATCAAAGTAGTAACGATGA+AGG | + | chr8.3:15968648-15968667 | MS.gene58434:CDS | 35.0% | |
| TATTAATCCTTTCCTCTTGG+TGG | - | chr8.3:15969139-15969158 | None:intergenic | 35.0% | |
| TCGAGATGCATAATCAGATT+TGG | - | chr8.3:15969054-15969073 | None:intergenic | 35.0% | |
| TTTGTTTCAGCTGATGAGTT+AGG | - | chr8.3:15968942-15968961 | None:intergenic | 35.0% | |
| !! | AAATGGCTTTTTGAAGATGG+GGG | + | chr8.3:15969203-15969222 | MS.gene58434:CDS | 35.0% |
| AGCACAACACAAGATCATCA+TGG | + | chr8.3:15969017-15969036 | MS.gene58434:CDS | 40.0% | |
| ATCGATATTCAGCAACATGC+AGG | + | chr8.3:15968968-15968987 | MS.gene58434:CDS | 40.0% | |
| CATGTTGCTGAATATCGATG+AGG | - | chr8.3:15968967-15968986 | None:intergenic | 40.0% | |
| CGATTTAGGTCAACTCATGA+AGG | + | chr8.3:15969071-15969090 | MS.gene58434:CDS | 40.0% | |
| GGTTGTAGGGTTATGTTGAA+GGG | - | chr8.3:15968778-15968797 | None:intergenic | 40.0% | |
| TAGCTTGATGCATATGGAGA+TGG | - | chr8.3:15968879-15968898 | None:intergenic | 40.0% | |
| TCGATATTCAGCAACATGCA+GGG | + | chr8.3:15968969-15968988 | MS.gene58434:CDS | 40.0% | |
| ! | GATGGAATATTCAGGTTTGG+TGG | - | chr8.3:15968861-15968880 | None:intergenic | 40.0% |
| ! | GGAGATGGAATATTCAGGTT+TGG | - | chr8.3:15968864-15968883 | None:intergenic | 40.0% |
| AAGGGACAAAGCCTCAGATA+AGG | - | chr8.3:15968760-15968779 | None:intergenic | 45.0% | |
| AGCTGCCGTATAAAGGTCAA+TGG | + | chr8.3:15968694-15968713 | MS.gene58434:CDS | 45.0% | |
| CAACATAACCCTACAACCCT+TGG | + | chr8.3:15968780-15968799 | MS.gene58434:CDS | 45.0% | |
| GATGAGGGTTTCATCTGATC+AGG | - | chr8.3:15968813-15968832 | None:intergenic | 45.0% | |
| GCTGCCGTATAAAGGTCAAT+GGG | + | chr8.3:15968695-15968714 | MS.gene58434:CDS | 45.0% | |
| GGGTTGTAGGGTTATGTTGA+AGG | - | chr8.3:15968779-15968798 | None:intergenic | 45.0% | |
| TCTTCTTCCACCAAGAGGAA+AGG | + | chr8.3:15969129-15969148 | MS.gene58434:CDS | 45.0% | |
| ! | AGCCTCAGATAAGGCTTGTT+TGG | - | chr8.3:15968751-15968770 | None:intergenic | 45.0% |
| ! | CAGGGATCAAGTTGTAGTGA+AGG | + | chr8.3:15968987-15969006 | MS.gene58434:CDS | 45.0% |
| ! | CTGATCAGGCAAAGTACCAA+GGG | - | chr8.3:15968799-15968818 | None:intergenic | 45.0% |
| ! | GCAAAGTACCAAGGGTTGTA+GGG | - | chr8.3:15968791-15968810 | None:intergenic | 45.0% |
| ! | TCTGATCAGGCAAAGTACCA+AGG | - | chr8.3:15968800-15968819 | None:intergenic | 45.0% |
| ! | GGCAAAGTACCAAGGGTTGT+AGG | - | chr8.3:15968792-15968811 | None:intergenic | 50.0% |
| ! | GGCCAAACAAGCCTTATCTG+AGG | + | chr8.3:15968746-15968765 | MS.gene58434:CDS | 50.0% |
| CGTTCGCAGCTGCCGTATAA+AGG | + | chr8.3:15968687-15968706 | MS.gene58434:CDS | 55.0% | |
| GGGTCTCTTCTTCCACCAAG+AGG | + | chr8.3:15969124-15969143 | MS.gene58434:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 15968639 | 15969295 | 15968639 | ID=MS.gene58434 |
| chr8.3 | mRNA | 15968639 | 15969295 | 15968639 | ID=MS.gene58434.t1;Parent=MS.gene58434 |
| chr8.3 | exon | 15968639 | 15969295 | 15968639 | ID=MS.gene58434.t1.exon1;Parent=MS.gene58434.t1 |
| chr8.3 | CDS | 15968639 | 15969295 | 15968639 | ID=cds.MS.gene58434.t1;Parent=MS.gene58434.t1 |
| Gene Sequence |
| Protein sequence |
