Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058790.t1 | XP_003598060.2 | 82.2 | 169 | 23 | 2 | 1 | 169 | 1 | 162 | 1.20E-74 | 289.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058790.t1 | F1MK05 | 40.4 | 114 | 58 | 4 | 14 | 126 | 84 | 188 | 9.9e-14 | 78.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058790.t1 | G7J068 | 82.2 | 169 | 23 | 2 | 1 | 169 | 1 | 162 | 8.5e-75 | 289.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049369 | MS.gene058790 | 0.814143 | 1.79E-51 | -1.69E-46 |
| MS.gene050686 | MS.gene058790 | 0.811155 | 8.06E-51 | -1.69E-46 |
| MS.gene051692 | MS.gene058790 | 0.815327 | 9.78E-52 | -1.69E-46 |
| MS.gene053449 | MS.gene058790 | 0.81204 | 5.18E-51 | -1.69E-46 |
| MS.gene053813 | MS.gene058790 | 0.829912 | 3.95E-55 | -1.69E-46 |
| MS.gene056422 | MS.gene058790 | 0.811809 | 5.82E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene058790 | 0.803221 | 3.87E-49 | -1.69E-46 |
| MS.gene057926 | MS.gene058790 | 0.835197 | 1.93E-56 | -1.69E-46 |
| MS.gene058790 | MS.gene059674 | 0.808591 | 2.87E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene060804 | 0.807377 | 5.21E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene060837 | 0.831282 | 1.82E-55 | -1.69E-46 |
| MS.gene058790 | MS.gene06142 | 0.805966 | 1.03E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene062897 | 0.828691 | 7.81E-55 | -1.69E-46 |
| MS.gene058790 | MS.gene064832 | 0.810401 | 1.17E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene06490 | 0.815271 | 1.01E-51 | -1.69E-46 |
| MS.gene058790 | MS.gene065204 | 0.833629 | 4.78E-56 | -1.69E-46 |
| MS.gene058790 | MS.gene065215 | 0.80731 | 5.38E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene065699 | 0.812744 | 3.63E-51 | -1.69E-46 |
| MS.gene058790 | MS.gene067332 | 0.81078 | 9.72E-51 | -1.69E-46 |
| MS.gene058790 | MS.gene068634 | 0.831312 | 1.79E-55 | -1.69E-46 |
| MS.gene058790 | MS.gene069435 | 0.840299 | 9.45E-58 | -1.69E-46 |
| MS.gene058790 | MS.gene069778 | 0.802209 | 6.25E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene071757 | 0.808253 | 3.39E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene071758 | 0.805826 | 1.11E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene08520 | 0.809054 | 2.29E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene08832 | 0.800232 | 1.59E-48 | -1.69E-46 |
| MS.gene058790 | MS.gene22737 | 0.821606 | 3.69E-53 | -1.69E-46 |
| MS.gene058790 | MS.gene25354 | 0.817301 | 3.54E-52 | -1.69E-46 |
| MS.gene058790 | MS.gene25651 | 0.815104 | 1.10E-51 | -1.69E-46 |
| MS.gene058790 | MS.gene25858 | 0.802497 | 5.45E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene28490 | 0.8347 | 2.58E-56 | -1.69E-46 |
| MS.gene058790 | MS.gene32666 | 0.801282 | 9.69E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene33504 | 0.81008 | 1.38E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene34376 | 0.802007 | 6.88E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene34496 | 0.807467 | 4.98E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene35966 | 0.823202 | 1.57E-53 | -1.69E-46 |
| MS.gene058790 | MS.gene36506 | 0.805157 | 1.53E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene37159 | 0.838138 | 3.44E-57 | -1.69E-46 |
| MS.gene058790 | MS.gene40212 | 0.805731 | 1.16E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene41435 | 0.805646 | 1.21E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene43839 | 0.826589 | 2.50E-54 | -1.69E-46 |
| MS.gene058790 | MS.gene46942 | 0.803781 | 2.96E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene47828 | 0.81169 | 6.17E-51 | -1.69E-46 |
| MS.gene058790 | MS.gene48593 | 0.801161 | 1.03E-48 | -1.69E-46 |
| MS.gene058790 | MS.gene49365 | 0.811483 | 6.85E-51 | -1.69E-46 |
| MS.gene058790 | MS.gene49500 | 0.832355 | 9.91E-56 | -1.69E-46 |
| MS.gene058790 | MS.gene49717 | 0.802731 | 4.88E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene51807 | 0.801968 | 7.01E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene51808 | 0.808208 | 3.47E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene51922 | 0.809234 | 2.09E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene52101 | 0.818478 | 1.92E-52 | -1.69E-46 |
| MS.gene058790 | MS.gene52283 | 0.803306 | 3.71E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene52774 | 0.810245 | 1.27E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene58120 | 0.824579 | 7.48E-54 | -1.69E-46 |
| MS.gene058790 | MS.gene58434 | 0.807385 | 5.19E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene58817 | 0.809092 | 2.24E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene59950 | 0.804457 | 2.14E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene61160 | 0.830218 | 3.32E-55 | -1.69E-46 |
| MS.gene058790 | MS.gene66434 | 0.808994 | 2.36E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene68423 | 0.803259 | 3.80E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene68677 | 0.807084 | 6.01E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene68856 | 0.817679 | 2.91E-52 | -1.69E-46 |
| MS.gene058790 | MS.gene68964 | 0.816749 | 4.71E-52 | -1.69E-46 |
| MS.gene058790 | MS.gene69489 | 0.802005 | 6.89E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene70170 | 0.806953 | 6.40E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene71667 | 0.82933 | 5.47E-55 | -1.69E-46 |
| MS.gene058790 | MS.gene73334 | 0.821623 | 3.66E-53 | -1.69E-46 |
| MS.gene058790 | MS.gene76346 | 0.824247 | 8.95E-54 | -1.69E-46 |
| MS.gene058790 | MS.gene76907 | 0.808401 | 3.15E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene79726 | 0.818043 | 2.41E-52 | -1.69E-46 |
| MS.gene058790 | MS.gene82008 | 0.820381 | 7.07E-53 | -1.69E-46 |
| MS.gene058790 | MS.gene84575 | 0.801859 | 7.38E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene86216 | 0.811082 | 8.36E-51 | -1.69E-46 |
| MS.gene058790 | MS.gene87404 | 0.839191 | 1.84E-57 | -1.69E-46 |
| MS.gene058790 | MS.gene87890 | 0.803229 | 3.85E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene90135 | 0.811264 | 7.64E-51 | -1.69E-46 |
| MS.gene058790 | MS.gene90788 | 0.810821 | 9.52E-51 | -1.69E-46 |
| MS.gene058790 | MS.gene93035 | 0.821759 | 3.40E-53 | -1.69E-46 |
| MS.gene058790 | MS.gene95702 | 0.809735 | 1.63E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058790.t1 | MTR_3g005790 | 82.840 | 169 | 22 | 2 | 1 | 169 | 1 | 162 | 2.82e-99 | 283 |
| MS.gene058790.t1 | MTR_3g005800 | 75.000 | 148 | 31 | 1 | 4 | 151 | 3 | 144 | 5.60e-77 | 228 |
| MS.gene058790.t1 | MTR_2g094040 | 51.908 | 131 | 57 | 3 | 24 | 148 | 69 | 199 | 1.71e-39 | 133 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058790.t1 | AT1G22510 | 54.688 | 128 | 54 | 3 | 24 | 149 | 10 | 135 | 2.54e-40 | 134 |
| MS.gene058790.t1 | AT1G72175 | 51.748 | 143 | 59 | 4 | 11 | 151 | 3 | 137 | 6.74e-40 | 133 |
| MS.gene058790.t1 | AT4G33940 | 32.857 | 140 | 87 | 2 | 4 | 143 | 60 | 192 | 1.20e-24 | 95.1 |
| MS.gene058790.t1 | AT4G33940 | 36.667 | 120 | 69 | 2 | 4 | 123 | 60 | 172 | 2.77e-24 | 95.5 |
| MS.gene058790.t1 | AT1G22510 | 49.533 | 107 | 50 | 3 | 45 | 149 | 49 | 153 | 4.28e-24 | 93.6 |
| MS.gene058790.t1 | AT1G72175 | 48.544 | 103 | 49 | 3 | 51 | 151 | 3 | 103 | 1.80e-21 | 85.5 |
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAGAGGAAAAGACATATT+AGG | 0.217850 | 3.3:+1366407 | None:intergenic |
| CAAACTGTATTATCCAAGTT+TGG | 0.336066 | 3.3:-1366661 | MS.gene058790:CDS |
| GTGAAAACAAGAAGATTAAT+AGG | 0.362944 | 3.3:+1366590 | None:intergenic |
| GAACATCGATCGTTTGTTGC+TGG | 0.371600 | 3.3:+1366761 | None:intergenic |
| CAGAGCTCGTGTCTTCATTA+CGG | 0.376209 | 3.3:-1366381 | MS.gene058790:intron |
| CAAGAGGAAAAGACATATTA+GGG | 0.384189 | 3.3:+1366408 | None:intergenic |
| CATCTCCAACACTCCGACTA+AGG | 0.436334 | 3.3:+1366245 | None:intergenic |
| GTCAGGCAAATTGCTCTCAC+TGG | 0.470875 | 3.3:-1366691 | MS.gene058790:CDS |
| TGAGCAATACTAGCATTAGA+TGG | 0.474337 | 3.3:+1366485 | None:intergenic |
| AAAAGCCTTCTTAAAAGAAA+CGG | 0.484810 | 3.3:+1366446 | None:intergenic |
| AAACTTCCAACTCCCATGTC+AGG | 0.507648 | 3.3:-1366708 | MS.gene058790:CDS |
| TGAGAGCAATTTGCCTGACA+TGG | 0.514810 | 3.3:+1366695 | None:intergenic |
| GAGAAGAATATTGCCAAACT+TGG | 0.518118 | 3.3:+1366648 | None:intergenic |
| AATTTGCCTGACATGGGAGT+TGG | 0.522076 | 3.3:+1366702 | None:intergenic |
| GGGAGGGAGAGAGATGGATG+TGG | 0.522212 | 3.3:-1366807 | None:intergenic |
| ATCGTTGTTGTCGTCAACAT+CGG | 0.562374 | 3.3:+1366568 | None:intergenic |
| GAGATCCTTAGTCGGAGTGT+TGG | 0.564250 | 3.3:-1366250 | MS.gene058790:CDS |
| GGACATTTGCAAGGATGAAG+AGG | 0.567032 | 3.3:+1366626 | None:intergenic |
| CGACAACAACGATTATGAGC+AGG | 0.579317 | 3.3:-1366558 | MS.gene058790:CDS |
| TGGAGATGACTTCGAACCGT+TGG | 0.579560 | 3.3:-1366230 | MS.gene058790:CDS |
| ACACGAGCTCTGATAACAAG+AGG | 0.581391 | 3.3:+1366392 | None:intergenic |
| CAGGTAGTTGTGAATCGTGT+CGG | 0.591095 | 3.3:-1366277 | MS.gene058790:CDS |
| GAGAGCAATTTGCCTGACAT+GGG | 0.611294 | 3.3:+1366696 | None:intergenic |
| CATCGATGTTCAATCTGTCA+CGG | 0.615902 | 3.3:-1366731 | MS.gene058790:CDS |
| TTAATAGGACGACGACATAG+AGG | 0.635128 | 3.3:+1366605 | None:intergenic |
| TGAGCAGGATCGTCTGATTG+TGG | 0.642021 | 3.3:-1366543 | MS.gene058790:CDS |
| CGACATAGAGGACATTTGCA+AGG | 0.645789 | 3.3:+1366617 | None:intergenic |
| GCTACTGATCATAGGACCAA+CGG | 0.665436 | 3.3:+1366214 | None:intergenic |
| TCGGATTTGAGATCCTTAGT+CGG | 0.681101 | 3.3:-1366258 | MS.gene058790:CDS |
| GATGTGGAGCATCATAGCAG+CGG | 0.708662 | 3.3:-1366791 | MS.gene058790:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATATTCAAATTATCATC+AGG | - | chr3.3:1366720-1366739 | MS.gene058790:CDS | 15.0% |
| ! | AAAAGCCTTCTTAAAAGAAA+CGG | + | chr3.3:1366573-1366592 | None:intergenic | 25.0% |
| ! | GTGAAAACAAGAAGATTAAT+AGG | + | chr3.3:1366429-1366448 | None:intergenic | 25.0% |
| ACAAGAGGAAAAGACATATT+AGG | + | chr3.3:1366612-1366631 | None:intergenic | 30.0% | |
| CAAACTGTATTATCCAAGTT+TGG | - | chr3.3:1366355-1366374 | MS.gene058790:intron | 30.0% | |
| CAAGAGGAAAAGACATATTA+GGG | + | chr3.3:1366611-1366630 | None:intergenic | 30.0% | |
| !! | ATCTTCCGTTTCTTTTAAGA+AGG | - | chr3.3:1366565-1366584 | MS.gene058790:CDS | 30.0% |
| ATAAACAACAACAACGACGA+CGG | + | chr3.3:1366705-1366724 | None:intergenic | 35.0% | |
| GAGAAGAATATTGCCAAACT+TGG | + | chr3.3:1366371-1366390 | None:intergenic | 35.0% | |
| TGAGCAATACTAGCATTAGA+TGG | + | chr3.3:1366534-1366553 | None:intergenic | 35.0% | |
| ATCGTTGTTGTCGTCAACAT+CGG | + | chr3.3:1366451-1366470 | None:intergenic | 40.0% | |
| CATCGATGTTCAATCTGTCA+CGG | - | chr3.3:1366285-1366304 | MS.gene058790:intron | 40.0% | |
| TTAATAGGACGACGACATAG+AGG | + | chr3.3:1366414-1366433 | None:intergenic | 40.0% | |
| ! | TCGGATTTGAGATCCTTAGT+CGG | - | chr3.3:1366758-1366777 | MS.gene058790:CDS | 40.0% |
| AAACTTCCAACTCCCATGTC+AGG | - | chr3.3:1366308-1366327 | MS.gene058790:intron | 45.0% | |
| AATTTGCCTGACATGGGAGT+TGG | + | chr3.3:1366317-1366336 | None:intergenic | 45.0% | |
| CAGAGCTCGTGTCTTCATTA+CGG | - | chr3.3:1366635-1366654 | MS.gene058790:CDS | 45.0% | |
| CAGGTAGTTGTGAATCGTGT+CGG | - | chr3.3:1366739-1366758 | MS.gene058790:CDS | 45.0% | |
| CGACAACAACGATTATGAGC+AGG | - | chr3.3:1366458-1366477 | MS.gene058790:CDS | 45.0% | |
| CGACATAGAGGACATTTGCA+AGG | + | chr3.3:1366402-1366421 | None:intergenic | 45.0% | |
| GAGAGCAATTTGCCTGACAT+GGG | + | chr3.3:1366323-1366342 | None:intergenic | 45.0% | |
| GGACATTTGCAAGGATGAAG+AGG | + | chr3.3:1366393-1366412 | None:intergenic | 45.0% | |
| TGAGAGCAATTTGCCTGACA+TGG | + | chr3.3:1366324-1366343 | None:intergenic | 45.0% | |
| ! | ACACGAGCTCTGATAACAAG+AGG | + | chr3.3:1366627-1366646 | None:intergenic | 45.0% |
| ! | GAACATCGATCGTTTGTTGC+TGG | + | chr3.3:1366258-1366277 | None:intergenic | 45.0% |
| CATCTCCAACACTCCGACTA+AGG | + | chr3.3:1366774-1366793 | None:intergenic | 50.0% | |
| GAGATCCTTAGTCGGAGTGT+TGG | - | chr3.3:1366766-1366785 | MS.gene058790:CDS | 50.0% | |
| GTCAGGCAAATTGCTCTCAC+TGG | - | chr3.3:1366325-1366344 | MS.gene058790:intron | 50.0% | |
| TGAGCAGGATCGTCTGATTG+TGG | - | chr3.3:1366473-1366492 | MS.gene058790:CDS | 50.0% | |
| TGGAGATGACTTCGAACCGT+TGG | - | chr3.3:1366786-1366805 | MS.gene058790:CDS | 50.0% | |
| ! | GATGTGGAGCATCATAGCAG+CGG | - | chr3.3:1366225-1366244 | MS.gene058790:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 1366222 | 1366816 | 1366222 | ID=MS.gene058790 |
| chr3.3 | mRNA | 1366222 | 1366816 | 1366222 | ID=MS.gene058790.t1;Parent=MS.gene058790 |
| chr3.3 | exon | 1366382 | 1366816 | 1366382 | ID=MS.gene058790.t1.exon1;Parent=MS.gene058790.t1 |
| chr3.3 | CDS | 1366382 | 1366816 | 1366382 | ID=cds.MS.gene058790.t1;Parent=MS.gene058790.t1 |
| chr3.3 | exon | 1366222 | 1366296 | 1366222 | ID=MS.gene058790.t1.exon2;Parent=MS.gene058790.t1 |
| chr3.3 | CDS | 1366222 | 1366296 | 1366222 | ID=cds.MS.gene058790.t1;Parent=MS.gene058790.t1 |
| Gene Sequence |
| Protein sequence |
