AlfalfaGEDB | MS.gene59006

M. sativa cultivar XinJiangDaYe / MS.gene59006

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59006.t1	XP_013453855.1	97.6	82	2	0	1	82	269	350	8.50E-37	162.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59006.t1	D4N502	60.0	80	32	0	1	80	281	360	3.4e-18	92.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59006.t1	A0A072UER0	97.6	82	2	0	1	82	269	350	6.1e-37	162.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049267	MS.gene59006	0.809467	1.87E-50	-1.69E-46
MS.gene049611	MS.gene59006	0.807508	4.88E-50	-1.69E-46
MS.gene049634	MS.gene59006	0.803529	3.34E-49	-1.69E-46
MS.gene050096	MS.gene59006	0.80632	8.71E-50	-1.69E-46
MS.gene050380	MS.gene59006	0.847796	9.22E-60	-1.69E-46
MS.gene050381	MS.gene59006	0.820086	8.26E-53	-1.69E-46
MS.gene050809	MS.gene59006	0.858293	9.10E-63	-1.69E-46
MS.gene05104	MS.gene59006	0.823815	1.13E-53	-1.69E-46
MS.gene051632	MS.gene59006	0.828399	9.18E-55	-1.69E-46
MS.gene05240	MS.gene59006	0.812807	3.52E-51	-1.69E-46
MS.gene052805	MS.gene59006	0.834819	2.40E-56	-1.69E-46
MS.gene053420	MS.gene59006	0.818201	2.22E-52	-1.69E-46
MS.gene054290	MS.gene59006	0.873007	2.05E-67	-1.69E-46
MS.gene054742	MS.gene59006	0.819377	1.20E-52	-1.69E-46
MS.gene055124	MS.gene59006	0.849932	2.35E-60	-1.69E-46
MS.gene055959	MS.gene59006	0.903247	4.22E-79	-1.69E-46
MS.gene056330	MS.gene59006	0.819745	9.88E-53	-1.69E-46
MS.gene056422	MS.gene59006	-0.808387	3.18E-50	-1.69E-46
MS.gene056856	MS.gene59006	0.802814	4.69E-49	-1.69E-46
MS.gene056920	MS.gene59006	0.807214	5.64E-50	-1.69E-46
MS.gene056923	MS.gene59006	0.8016	8.34E-49	-1.69E-46
MS.gene057544	MS.gene59006	0.841803	3.81E-58	-1.69E-46
MS.gene058067	MS.gene59006	0.806687	7.29E-50	-1.69E-46
MS.gene058093	MS.gene59006	0.89561	8.14E-76	-1.69E-46
MS.gene058099	MS.gene59006	0.847918	8.53E-60	-1.69E-46
MS.gene058108	MS.gene59006	0.803931	2.75E-49	-1.69E-46
MS.gene058109	MS.gene59006	0.802687	4.98E-49	-1.69E-46
MS.gene058171	MS.gene59006	0.805637	1.21E-49	-1.69E-46
MS.gene058175	MS.gene59006	0.804308	2.30E-49	-1.69E-46
MS.gene058176	MS.gene59006	0.823259	1.52E-53	-1.69E-46
MS.gene058177	MS.gene59006	0.812195	4.79E-51	-1.69E-46
MS.gene058179	MS.gene59006	0.887914	9.38E-73	-1.69E-46
MS.gene058180	MS.gene59006	0.849901	2.40E-60	-1.69E-46
MS.gene058218	MS.gene59006	0.813651	2.30E-51	-1.69E-46
MS.gene058840	MS.gene59006	0.860378	2.15E-63	-1.69E-46
MS.gene059037	MS.gene59006	0.887877	9.70E-73	-1.69E-46
MS.gene059038	MS.gene59006	0.856979	2.23E-62	-1.69E-46
MS.gene059045	MS.gene59006	0.80943	1.90E-50	-1.69E-46
MS.gene059402	MS.gene59006	0.821676	3.56E-53	-1.69E-46
MS.gene059944	MS.gene59006	0.808402	3.15E-50	-1.69E-46
MS.gene060205	MS.gene59006	0.824621	7.31E-54	-1.69E-46
MS.gene060578	MS.gene59006	0.8326	8.62E-56	-1.69E-46
MS.gene06074	MS.gene59006	0.808243	3.41E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59006.t1	MTR_5g048550	97.561	82	2	0	1	82	269	350	6.74e-53	168
MS.gene59006.t1	MTR_5g055680	73.171	82	22	0	1	82	269	350	1.58e-41	139
MS.gene59006.t1	MTR_0009s0050	71.951	82	23	0	1	82	89	170	7.23e-40	130
MS.gene59006.t1	MTR_0009s0050	71.951	82	23	0	1	82	171	252	5.22e-39	130
MS.gene59006.t1	MTR_0009s0050	71.951	82	23	0	1	82	171	252	1.13e-38	129
MS.gene59006.t1	MTR_0009s0050	71.951	82	23	0	1	82	269	350	1.93e-38	130
MS.gene59006.t1	MTR_0009s0050	71.951	82	23	0	1	82	269	350	2.92e-38	130
MS.gene59006.t1	MTR_5g055900	69.136	81	25	0	1	81	269	349	3.71e-37	127
MS.gene59006.t1	MTR_5g048570	69.512	82	25	0	1	82	267	348	6.08e-37	127
MS.gene59006.t1	MTR_0056s0160	70.732	82	24	0	1	82	154	235	1.99e-36	122
MS.gene59006.t1	MTR_0009s0400	63.095	84	31	0	1	84	65	148	7.27e-35	116
MS.gene59006.t1	MTR_8g064200	68.293	82	26	0	1	82	280	361	1.88e-34	120
MS.gene59006.t1	MTR_8g064200	68.293	82	26	0	1	82	275	356	2.05e-34	120
MS.gene59006.t1	MTR_5g048470	65.432	81	28	0	1	81	276	356	1.68e-32	115
MS.gene59006.t1	MTR_0009s0480	59.524	84	34	0	1	84	268	351	1.29e-31	113
MS.gene59006.t1	MTR_5g048850	60.714	84	33	0	1	84	269	352	1.41e-31	113
MS.gene59006.t1	MTR_5g055820	61.728	81	31	0	1	81	270	350	7.76e-31	111
MS.gene59006.t1	MTR_5g055800	63.415	82	29	1	1	82	269	349	4.20e-30	108
MS.gene59006.t1	MTR_5g048740	59.524	84	34	0	1	84	268	351	4.78e-30	108
MS.gene59006.t1	MTR_0328s0030	60.465	86	34	0	1	86	267	352	6.59e-30	108
MS.gene59006.t1	MTR_5g055690	61.728	81	31	0	1	81	270	350	1.88e-29	107
MS.gene59006.t1	MTR_2g072910	59.756	82	33	0	1	82	275	356	1.29e-27	102
MS.gene59006.t1	MTR_2g072890	54.878	82	37	0	1	82	276	357	8.00e-26	97.8
MS.gene59006.t1	MTR_1g033810	54.545	88	38	2	1	86	271	358	3.03e-21	85.5
MS.gene59006.t1	MTR_5g065010	44.444	81	44	1	1	80	262	342	7.64e-18	76.3
MS.gene59006.t1	MTR_3g063180	36.585	82	52	0	1	82	273	354	1.94e-16	72.4
MS.gene59006.t1	MTR_2g437380	40.741	81	48	0	1	81	183	263	3.39e-16	70.9
MS.gene59006.t1	MTR_2g437380	40.741	81	48	0	1	81	277	357	8.73e-16	70.5
MS.gene59006.t1	MTR_7g062600	35.714	84	54	0	1	84	274	357	3.62e-14	65.9
MS.gene59006.t1	MTR_7g063730	35.802	81	52	0	1	81	286	366	1.15e-13	64.7
MS.gene59006.t1	MTR_3g070860	36.709	79	50	0	1	79	278	356	1.64e-13	64.3
MS.gene59006.t1	MTR_7g062550	35.802	81	52	0	1	81	282	362	2.63e-13	63.5
MS.gene59006.t1	MTR_8g024120	33.750	80	53	0	1	80	264	343	3.19e-13	63.2
MS.gene59006.t1	MTR_7g062580	34.524	84	55	0	1	84	277	360	5.56e-13	62.8
MS.gene59006.t1	MTR_7g062570	35.802	81	52	0	1	81	277	357	1.02e-12	62.0
MS.gene59006.t1	MTR_7g068650	41.667	60	35	0	1	60	283	342	1.51e-12	61.6
MS.gene59006.t1	MTR_4g123020	40.506	79	46	1	1	79	242	319	2.73e-12	60.5
MS.gene59006.t1	MTR_3g092890	34.615	78	51	0	2	79	259	336	2.98e-12	60.5
MS.gene59006.t1	MTR_5g082750	35.443	79	51	0	1	79	277	355	3.76e-12	60.5
MS.gene59006.t1	MTR_4g015790	40.000	60	36	0	1	60	291	350	4.85e-12	60.1
MS.gene59006.t1	MTR_8g074050	37.500	80	48	1	1	80	277	354	6.62e-12	59.7
MS.gene59006.t1	MTR_3g026150	36.250	80	50	1	1	80	282	360	7.81e-12	59.3
MS.gene59006.t1	MTR_3g026150	36.250	80	50	1	1	80	179	257	8.96e-12	58.9
MS.gene59006.t1	MTR_1g032140	33.333	81	52	2	1	79	284	364	1.16e-11	58.9
MS.gene59006.t1	MTR_8g074070	38.750	80	47	1	1	80	277	354	1.99e-11	58.2
MS.gene59006.t1	MTR_8g074070	38.750	80	47	1	1	80	258	335	2.48e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59006.t1	AT1G78550	55.000	80	36	0	1	80	199	278	1.53e-25	95.9
MS.gene59006.t1	AT1G78550	55.000	80	36	0	1	80	277	356	5.91e-25	95.5
MS.gene59006.t1	AT4G25300	53.750	80	37	0	1	80	183	262	1.68e-23	90.1
MS.gene59006.t1	AT1G17010	53.086	81	38	0	1	81	279	359	3.54e-23	90.9
MS.gene59006.t1	AT4G25300	53.750	80	37	0	1	80	277	356	1.28e-22	89.4
MS.gene59006.t1	AT4G25310	51.250	80	39	0	1	80	246	325	7.58e-22	86.7
MS.gene59006.t1	AT4G25310	51.250	80	39	0	1	80	274	353	1.77e-21	86.3
MS.gene59006.t1	AT1G17020	50.000	80	40	0	1	80	279	358	5.28e-20	82.0
MS.gene59006.t1	AT3G21420	39.759	83	49	1	1	82	282	364	9.47e-15	67.8
MS.gene59006.t1	AT4G16330	37.179	78	45	1	1	78	183	256	3.92e-13	62.8
MS.gene59006.t1	AT4G16330	37.179	78	45	1	1	78	289	362	4.56e-13	63.2
MS.gene59006.t1	AT4G10490	32.099	81	53	1	1	79	264	344	1.69e-12	61.2
MS.gene59006.t1	AT2G38240	38.750	80	49	0	1	80	272	351	9.04e-12	59.3
MS.gene59006.t1	AT3G13610	28.750	80	57	0	1	80	282	361	4.43e-11	57.4
MS.gene59006.t1	AT3G60290	34.940	83	52	2	1	82	275	356	5.54e-11	57.0

Find 19 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAATGATGCAGGTCTTTC+AGG	0.141622	5.1:-37536130	None:intergenic
TTTCCATTGCTGCATTTCAT+AGG	0.363746	5.1:+37536062	MS.gene59006:CDS
TATTTCTCACGCAAGCTAGA+AGG	0.431799	5.1:+37536184	MS.gene59006:CDS
TTGCTCGATGTTCAATGCTT+CGG	0.438986	5.1:-37536015	None:intergenic
CAGTTAATTCAGAGAAAGAA+AGG	0.449951	5.1:+37536038	MS.gene59006:CDS
TGAATGATGCAGGTCTTTCA+GGG	0.467010	5.1:-37536129	None:intergenic
CTTTCAGGGGTAACAAGACT+TGG	0.468383	5.1:-37536115	None:intergenic
AGTCTTGTCTTGACGTAATG+AGG	0.481535	5.1:+37536209	MS.gene59006:CDS
TCATTCAAAGGTATTGCTGT+TGG	0.492628	5.1:+37536145	MS.gene59006:CDS
GACGTAATGAGGTTGGAAAA+TGG	0.509343	5.1:+37536220	MS.gene59006:CDS
GGGGTAACAAGACTTGGTCT+TGG	0.516646	5.1:-37536109	None:intergenic
GCAATACCTTTGAATGATGC+AGG	0.526918	5.1:-37536139	None:intergenic
TGCGTGAGAAATATGCTTTG+AGG	0.529244	5.1:-37536174	None:intergenic
TTGTCTTGACGTAATGAGGT+TGG	0.531648	5.1:+37536213	MS.gene59006:CDS
GTATTACAGATAGTGACAAA+TGG	0.550244	5.1:+37535986	None:intergenic
CTTAAAATTGTATTCACATG+AGG	0.572204	5.1:-37536085	None:intergenic
GAAAGACCTGCATCATTCAA+AGG	0.591981	5.1:+37536133	MS.gene59006:CDS
AGGCCTATGAAATGCAGCAA+TGG	0.610164	5.1:-37536065	None:intergenic
GAATGATGCAGGTCTTTCAG+GGG	0.634371	5.1:-37536128	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTTAAAATTGTATTCACATG+AGG	-	chr5.1:37536088-37536107	None:intergenic	25.0%
	CAGTTAATTCAGAGAAAGAA+AGG	+	chr5.1:37536038-37536057	MS.gene59006:CDS	30.0%
	TTTCCATTGCTGCATTTCAT+AGG	+	chr5.1:37536062-37536081	MS.gene59006:CDS	35.0%
!!	TCATTCAAAGGTATTGCTGT+TGG	+	chr5.1:37536145-37536164	MS.gene59006:CDS	35.0%
	GAAAGACCTGCATCATTCAA+AGG	+	chr5.1:37536133-37536152	MS.gene59006:CDS	40.0%
	GACGTAATGAGGTTGGAAAA+TGG	+	chr5.1:37536220-37536239	MS.gene59006:CDS	40.0%
	GCAATACCTTTGAATGATGC+AGG	-	chr5.1:37536142-37536161	None:intergenic	40.0%
	TATTTCTCACGCAAGCTAGA+AGG	+	chr5.1:37536184-37536203	MS.gene59006:CDS	40.0%
	TGAATGATGCAGGTCTTTCA+GGG	-	chr5.1:37536132-37536151	None:intergenic	40.0%
	TGCGTGAGAAATATGCTTTG+AGG	-	chr5.1:37536177-37536196	None:intergenic	40.0%
	TTGAATGATGCAGGTCTTTC+AGG	-	chr5.1:37536133-37536152	None:intergenic	40.0%
	TTGCTCGATGTTCAATGCTT+CGG	-	chr5.1:37536018-37536037	None:intergenic	40.0%
	TTGTCTTGACGTAATGAGGT+TGG	+	chr5.1:37536213-37536232	MS.gene59006:CDS	40.0%
!	AGTCTTGTCTTGACGTAATG+AGG	+	chr5.1:37536209-37536228	MS.gene59006:CDS	40.0%
	AGGCCTATGAAATGCAGCAA+TGG	-	chr5.1:37536068-37536087	None:intergenic	45.0%
	CTTTCAGGGGTAACAAGACT+TGG	-	chr5.1:37536118-37536137	None:intergenic	45.0%
	GAATGATGCAGGTCTTTCAG+GGG	-	chr5.1:37536131-37536150	None:intergenic	45.0%
!	GGGGTAACAAGACTTGGTCT+TGG	-	chr5.1:37536112-37536131	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	37535995	37536255	37535995	ID=MS.gene59006
chr5.1	mRNA	37535995	37536255	37535995	ID=MS.gene59006.t1;Parent=MS.gene59006
chr5.1	exon	37535995	37536255	37535995	ID=MS.gene59006.t1.exon1;Parent=MS.gene59006.t1
chr5.1	CDS	37535995	37536255	37535995	ID=cds.MS.gene59006.t1;Parent=MS.gene59006.t1

Gene Sequence	>MS.gene59006 ATAGTGACAAATGGAATATACCGAAGCATTGAACATCGAGCAACAGTTAATTCAGAGAAAGAAAGGATTTCCATTGCTGCATTTCATAGGCCTCATGTGAATACAATTTTAAGTCCAAGACCAAGTCTTGTTACCCCTGAAAGACCTGCATCATTCAAAGGTATTGCTGTTGGAGATTACCTCAAAGCATATTTCTCACGCAAGCTAGAAGGAAAGTCTTGTCTTGACGTAATGAGGTTGGAAAATGGTGTTAAAAACTGA
Protein sequence	>MS.gene59006.t1 IVTNGIYRSIEHRATVNSEKERISIAAFHRPHVNTILSPRPSLVTPERPASFKGIAVGDYLKAYFSRKLEGKSCLDVMRLENGVKN