Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62926.t1 | AFK33681.1 | 95 | 119 | 6 | 0 | 1 | 119 | 103 | 221 | 7.60E-20 | 106.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62926.t1 | I3S089 | 95.0 | 119 | 6 | 0 | 1 | 119 | 103 | 221 | 5.5e-20 | 106.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene62926 | 0.851717 | 7.40E-61 | -1.69E-46 |
| MS.gene04919 | MS.gene62926 | 0.828384 | 9.26E-55 | -1.69E-46 |
| MS.gene04920 | MS.gene62926 | 0.850246 | 1.92E-60 | -1.69E-46 |
| MS.gene049283 | MS.gene62926 | 0.81955 | 1.10E-52 | -1.69E-46 |
| MS.gene049499 | MS.gene62926 | 0.842712 | 2.19E-58 | -1.69E-46 |
| MS.gene049680 | MS.gene62926 | 0.847856 | 8.87E-60 | -1.69E-46 |
| MS.gene05011 | MS.gene62926 | 0.800943 | 1.14E-48 | -1.69E-46 |
| MS.gene050752 | MS.gene62926 | 0.800386 | 1.48E-48 | -1.69E-46 |
| MS.gene050945 | MS.gene62926 | 0.83771 | 4.43E-57 | -1.69E-46 |
| MS.gene050955 | MS.gene62926 | 0.813467 | 2.52E-51 | -1.69E-46 |
| MS.gene051003 | MS.gene62926 | 0.830005 | 3.75E-55 | -1.69E-46 |
| MS.gene051043 | MS.gene62926 | 0.807467 | 4.98E-50 | -1.69E-46 |
| MS.gene051234 | MS.gene62926 | 0.818121 | 2.31E-52 | -1.69E-46 |
| MS.gene051343 | MS.gene62926 | 0.803125 | 4.05E-49 | -1.69E-46 |
| MS.gene051597 | MS.gene62926 | 0.809418 | 1.91E-50 | -1.69E-46 |
| MS.gene051699 | MS.gene62926 | 0.809629 | 1.72E-50 | -1.69E-46 |
| MS.gene05188 | MS.gene62926 | 0.809141 | 2.19E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene62926 | 0.817768 | 2.78E-52 | -1.69E-46 |
| MS.gene052072 | MS.gene62926 | 0.841409 | 4.84E-58 | -1.69E-46 |
| MS.gene052518 | MS.gene62926 | 0.800094 | 1.69E-48 | -1.69E-46 |
| MS.gene052533 | MS.gene62926 | 0.80967 | 1.69E-50 | -1.69E-46 |
| MS.gene052793 | MS.gene62926 | 0.816904 | 4.35E-52 | -1.69E-46 |
| MS.gene053272 | MS.gene62926 | -0.801613 | 8.29E-49 | -1.69E-46 |
| MS.gene053931 | MS.gene62926 | 0.80437 | 2.23E-49 | -1.69E-46 |
| MS.gene054004 | MS.gene62926 | 0.810633 | 1.05E-50 | -1.69E-46 |
| MS.gene054403 | MS.gene62926 | 0.809107 | 2.23E-50 | -1.69E-46 |
| MS.gene054413 | MS.gene62926 | 0.820633 | 6.19E-53 | -1.69E-46 |
| MS.gene054957 | MS.gene62926 | 0.8066 | 7.60E-50 | -1.69E-46 |
| MS.gene055150 | MS.gene62926 | 0.829339 | 5.44E-55 | -1.69E-46 |
| MS.gene055765 | MS.gene62926 | 0.80735 | 5.28E-50 | -1.69E-46 |
| MS.gene05689 | MS.gene62926 | 0.823292 | 1.50E-53 | -1.69E-46 |
| MS.gene056986 | MS.gene62926 | 0.83364 | 4.75E-56 | -1.69E-46 |
| MS.gene057102 | MS.gene62926 | 0.842536 | 2.44E-58 | -1.69E-46 |
| MS.gene057622 | MS.gene62926 | 0.800937 | 1.14E-48 | -1.69E-46 |
| MS.gene058159 | MS.gene62926 | 0.836331 | 9.96E-57 | -1.69E-46 |
| MS.gene058781 | MS.gene62926 | 0.826776 | 2.25E-54 | -1.69E-46 |
| MS.gene058783 | MS.gene62926 | 0.804406 | 2.19E-49 | -1.69E-46 |
| MS.gene05878 | MS.gene62926 | 0.804093 | 2.55E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene62926 | 0.877723 | 5.00E-69 | -1.69E-46 |
| MS.gene05952 | MS.gene62926 | 0.827583 | 1.44E-54 | -1.69E-46 |
| MS.gene059802 | MS.gene62926 | 0.803962 | 2.71E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene62926 | -0.870841 | 1.08E-66 | -1.69E-46 |
| MS.gene059901 | MS.gene62926 | -0.888174 | 7.46E-73 | -1.69E-46 |
| MS.gene059935 | MS.gene62926 | 0.806916 | 6.52E-50 | -1.69E-46 |
| MS.gene06011 | MS.gene62926 | 0.810846 | 9.41E-51 | -1.69E-46 |
| MS.gene060512 | MS.gene62926 | 0.804176 | 2.45E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene62926 | 0.849229 | 3.70E-60 | -1.69E-46 |
| MS.gene060933 | MS.gene62926 | 0.803947 | 2.73E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene62926 | 0.852859 | 3.50E-61 | -1.69E-46 |
| MS.gene060937 | MS.gene62926 | 0.855671 | 5.39E-62 | -1.69E-46 |
| MS.gene061100 | MS.gene62926 | 0.808051 | 3.74E-50 | -1.69E-46 |
| MS.gene061243 | MS.gene62926 | 0.805855 | 1.09E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62926.t1 | MTR_4g049540 | 99.160 | 119 | 1 | 0 | 1 | 119 | 103 | 221 | 1.64e-81 | 238 |
| MS.gene62926.t1 | MTR_2g088060 | 63.559 | 118 | 43 | 0 | 1 | 118 | 104 | 221 | 1.69e-46 | 149 |
| MS.gene62926.t1 | MTR_2g023880 | 55.932 | 118 | 52 | 0 | 1 | 118 | 104 | 221 | 4.03e-41 | 135 |
| MS.gene62926.t1 | MTR_7g056133 | 38.532 | 109 | 60 | 1 | 1 | 102 | 104 | 212 | 6.09e-15 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62926.t1 | AT5G42570 | 71.818 | 110 | 31 | 0 | 1 | 110 | 103 | 212 | 3.29e-49 | 156 |
| MS.gene62926.t1 | AT1G11905 | 52.336 | 107 | 51 | 0 | 1 | 107 | 103 | 209 | 1.05e-29 | 106 |
| MS.gene62926.t1 | AT1G11905 | 50.000 | 102 | 51 | 0 | 6 | 107 | 107 | 208 | 7.61e-26 | 96.7 |
Find 21 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAATCTGAGGGGTTCCTTA+TGG | 0.339577 | 4.2:-52091147 | MS.gene62926:CDS |
| TTTACCGTCCTCAAAACTTC+GGG | 0.391593 | 4.2:+52091310 | None:intergenic |
| TGGAAGACAATCAGAATCTT+CGG | 0.449833 | 4.2:-52091109 | MS.gene62926:CDS |
| TTGTTTCTTAACAGCTTCCA+TGG | 0.457783 | 4.2:+52091334 | None:intergenic |
| TGGAAGCACAGAGGAGCAAA+AGG | 0.480927 | 4.2:-52091285 | MS.gene62926:CDS |
| GAATCTGAATGCGAGGCAAA+AGG | 0.494465 | 4.2:-52091215 | MS.gene62926:CDS |
| TATGGAATATGATCGCCTCT+TGG | 0.494710 | 4.2:-52091129 | MS.gene62926:CDS |
| AGAGGCGATCATATTCCATA+AGG | 0.520476 | 4.2:+52091132 | None:intergenic |
| TATCAAGATGTGAAGAGCTC+TGG | 0.537183 | 4.2:+52091066 | None:intergenic |
| TAAGAGAGCTTCGTTTACTC+AGG | 0.542022 | 4.2:-52091361 | MS.gene62926:CDS |
| GACATTGGAAACTGAAGTAG+AGG | 0.553403 | 4.2:-52091180 | MS.gene62926:CDS |
| TCAGAATCTTCGGAGTCAGT+TGG | 0.558726 | 4.2:-52091099 | MS.gene62926:CDS |
| AGGAACCAAGGAAAAGACAT+TGG | 0.565077 | 4.2:-52091195 | MS.gene62926:CDS |
| ATGTGAAGAGCTCTGGTCGA+TGG | 0.573741 | 4.2:+52091073 | None:intergenic |
| GAATCTTCGGAGTCAGTTGG+AGG | 0.578067 | 4.2:-52091096 | MS.gene62926:CDS |
| AACGAAGCTCTCTTATGTAG+TGG | 0.615932 | 4.2:+52091369 | None:intergenic |
| TCGTTTACTCAGGAAGACCA+TGG | 0.634666 | 4.2:-52091351 | MS.gene62926:CDS |
| GAAGCTCGAATCTGAATGCG+AGG | 0.639033 | 4.2:-52091222 | MS.gene62926:CDS |
| GATTCTGATTGTCTTCCAAG+AGG | 0.650535 | 4.2:+52091114 | None:intergenic |
| ATGCGAGGCAAAAGGAACCA+AGG | 0.675638 | 4.2:-52091207 | MS.gene62926:CDS |
| CTTAATCTTAGACTTCAATG+TGG | 0.693873 | 4.2:+52091244 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTTAATCTTAGACTTCAATG+TGG | + | chr4.2:52091194-52091213 | None:intergenic | 30.0% | |
| CTCTTAAAAAGCAATCTGAG+GGG | - | chr4.2:52091278-52091297 | MS.gene62926:CDS | 35.0% | |
| GCTCTTAAAAAGCAATCTGA+GGG | - | chr4.2:52091277-52091296 | MS.gene62926:CDS | 35.0% | |
| TGGAAGACAATCAGAATCTT+CGG | - | chr4.2:52091326-52091345 | MS.gene62926:CDS | 35.0% | |
| ! | AGTTTCCAATGTCTTTTCCT+TGG | + | chr4.2:52091248-52091267 | None:intergenic | 35.0% |
| ! | TTGTTTCTTAACAGCTTCCA+TGG | + | chr4.2:52091104-52091123 | None:intergenic | 35.0% |
| ! | TTTTACCGTCCTCAAAACTT+CGG | + | chr4.2:52091129-52091148 | None:intergenic | 35.0% |
| !!! | AGTTTTGAGGACGGTAAAAA+TGG | - | chr4.2:52091130-52091149 | MS.gene62926:CDS | 35.0% |
| AACGAAGCTCTCTTATGTAG+TGG | + | chr4.2:52091069-52091088 | None:intergenic | 40.0% | |
| AGAGGCGATCATATTCCATA+AGG | + | chr4.2:52091306-52091325 | None:intergenic | 40.0% | |
| AGGAACCAAGGAAAAGACAT+TGG | - | chr4.2:52091240-52091259 | MS.gene62926:CDS | 40.0% | |
| GACATTGGAAACTGAAGTAG+AGG | - | chr4.2:52091255-52091274 | MS.gene62926:CDS | 40.0% | |
| GATTCTGATTGTCTTCCAAG+AGG | + | chr4.2:52091324-52091343 | None:intergenic | 40.0% | |
| GGCTCTTAAAAAGCAATCTG+AGG | - | chr4.2:52091276-52091295 | MS.gene62926:CDS | 40.0% | |
| TAAGAGAGCTTCGTTTACTC+AGG | - | chr4.2:52091074-52091093 | MS.gene62926:CDS | 40.0% | |
| TATCAAGATGTGAAGAGCTC+TGG | + | chr4.2:52091372-52091391 | None:intergenic | 40.0% | |
| TATGGAATATGATCGCCTCT+TGG | - | chr4.2:52091306-52091325 | MS.gene62926:CDS | 40.0% | |
| TTTACCGTCCTCAAAACTTC+GGG | + | chr4.2:52091128-52091147 | None:intergenic | 40.0% | |
| ! | GAAACAAACCCGAAGTTTTG+AGG | - | chr4.2:52091117-52091136 | MS.gene62926:CDS | 40.0% |
| GAATCTGAATGCGAGGCAAA+AGG | - | chr4.2:52091220-52091239 | MS.gene62926:CDS | 45.0% | |
| TCAGAATCTTCGGAGTCAGT+TGG | - | chr4.2:52091336-52091355 | MS.gene62926:CDS | 45.0% | |
| TCGTTTACTCAGGAAGACCA+TGG | - | chr4.2:52091084-52091103 | MS.gene62926:CDS | 45.0% | |
| ! | CGGTAAAAATGGAAGCACAG+AGG | - | chr4.2:52091141-52091160 | MS.gene62926:CDS | 45.0% |
| !!! | CAAACCCGAAGTTTTGAGGA+CGG | - | chr4.2:52091121-52091140 | MS.gene62926:CDS | 45.0% |
| ATGCGAGGCAAAAGGAACCA+AGG | - | chr4.2:52091228-52091247 | MS.gene62926:CDS | 50.0% | |
| GAAGCTCGAATCTGAATGCG+AGG | - | chr4.2:52091213-52091232 | MS.gene62926:CDS | 50.0% | |
| GAATCTTCGGAGTCAGTTGG+AGG | - | chr4.2:52091339-52091358 | MS.gene62926:CDS | 50.0% | |
| GCAATCTGAGGGGTTCCTTA+TGG | - | chr4.2:52091288-52091307 | MS.gene62926:CDS | 50.0% | |
| ! | ATGTGAAGAGCTCTGGTCGA+TGG | + | chr4.2:52091365-52091384 | None:intergenic | 50.0% |
| ! | TGGAAGCACAGAGGAGCAAA+AGG | - | chr4.2:52091150-52091169 | MS.gene62926:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 52091049 | 52091408 | 52091049 | ID=MS.gene62926 |
| chr4.2 | mRNA | 52091049 | 52091408 | 52091049 | ID=MS.gene62926.t1;Parent=MS.gene62926 |
| chr4.2 | exon | 52091049 | 52091408 | 52091049 | ID=MS.gene62926.t1.exon1;Parent=MS.gene62926.t1 |
| chr4.2 | CDS | 52091049 | 52091408 | 52091049 | ID=cds.MS.gene62926.t1;Parent=MS.gene62926.t1 |
| Gene Sequence |
| Protein sequence |
