AlfalfaGEDB | MS.gene64347

M. sativa cultivar XinJiangDaYe / MS.gene64347

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64347.t1	PNX75878.1	61.1	90	31	1	1	86	1	90	1.20E-14	89

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64347.t1	A0A2K3LBG3	61.1	90	31	1	1	86	1	90	8.6e-15	89.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049232	MS.gene64347	0.841393	4.88E-58	-1.69E-46
MS.gene049552	MS.gene64347	0.801813	7.54E-49	-1.69E-46
MS.gene050170	MS.gene64347	0.842344	2.74E-58	-1.69E-46
MS.gene050216	MS.gene64347	0.810654	1.04E-50	-1.69E-46
MS.gene050323	MS.gene64347	0.838308	3.11E-57	-1.69E-46
MS.gene050381	MS.gene64347	-0.809409	1.92E-50	-1.69E-46
MS.gene050496	MS.gene64347	0.857575	1.49E-62	-1.69E-46
MS.gene050502	MS.gene64347	0.805976	1.03E-49	-1.69E-46
MS.gene050504	MS.gene64347	0.819346	1.22E-52	-1.69E-46
MS.gene050577	MS.gene64347	0.805527	1.28E-49	-1.69E-46
MS.gene050612	MS.gene64347	0.80109	1.06E-48	-1.69E-46
MS.gene050675	MS.gene64347	0.825484	4.57E-54	-1.69E-46
MS.gene050697	MS.gene64347	0.824383	8.31E-54	-1.69E-46
MS.gene050868	MS.gene64347	0.85059	1.54E-60	-1.69E-46
MS.gene050871	MS.gene64347	0.85319	2.82E-61	-1.69E-46
MS.gene050986	MS.gene64347	0.812125	4.96E-51	-1.69E-46
MS.gene051268	MS.gene64347	0.813635	2.32E-51	-1.69E-46
MS.gene051509	MS.gene64347	0.816352	5.78E-52	-1.69E-46
MS.gene051560	MS.gene64347	0.811076	8.39E-51	-1.69E-46
MS.gene051874	MS.gene64347	0.801584	8.41E-49	-1.69E-46
MS.gene051941	MS.gene64347	0.817166	3.80E-52	-1.69E-46
MS.gene052164	MS.gene64347	0.814698	1.35E-51	-1.69E-46
MS.gene052482	MS.gene64347	0.823498	1.34E-53	-1.69E-46
MS.gene052486	MS.gene64347	0.824497	7.82E-54	-1.69E-46
MS.gene053203	MS.gene64347	0.810937	8.99E-51	-1.69E-46
MS.gene053420	MS.gene64347	-0.825977	3.49E-54	-1.69E-46
MS.gene053574	MS.gene64347	0.803733	3.03E-49	-1.69E-46
MS.gene053626	MS.gene64347	0.810717	1.00E-50	-1.69E-46
MS.gene053824	MS.gene64347	0.802697	4.96E-49	-1.69E-46
MS.gene054003	MS.gene64347	0.808526	2.97E-50	-1.69E-46
MS.gene054595	MS.gene64347	0.830722	2.50E-55	-1.69E-46
MS.gene054954	MS.gene64347	0.816314	5.89E-52	-1.69E-46
MS.gene055502	MS.gene64347	0.813464	2.53E-51	-1.69E-46
MS.gene05589	MS.gene64347	0.827457	1.55E-54	-1.69E-46
MS.gene056166	MS.gene64347	0.807824	4.19E-50	-1.69E-46
MS.gene056387	MS.gene64347	0.82531	5.03E-54	-1.69E-46
MS.gene056504	MS.gene64347	0.851919	6.49E-61	-1.69E-46
MS.gene056510	MS.gene64347	0.812104	5.01E-51	-1.69E-46
MS.gene057076	MS.gene64347	0.803122	4.05E-49	-1.69E-46
MS.gene057086	MS.gene64347	0.817241	3.65E-52	-1.69E-46
MS.gene05787	MS.gene64347	0.822641	2.12E-53	-1.69E-46
MS.gene058157	MS.gene64347	0.839006	2.05E-57	-1.69E-46
MS.gene058171	MS.gene64347	-0.826313	2.91E-54	-1.69E-46
MS.gene058173	MS.gene64347	-0.826479	2.65E-54	-1.69E-46
MS.gene058175	MS.gene64347	-0.825223	5.27E-54	-1.69E-46
MS.gene058269	MS.gene64347	-0.806438	8.22E-50	-1.69E-46
MS.gene05837	MS.gene64347	0.814702	1.35E-51	-1.69E-46
MS.gene059022	MS.gene64347	0.807469	4.98E-50	-1.69E-46
MS.gene059045	MS.gene64347	-0.810289	1.24E-50	-1.69E-46
MS.gene059223	MS.gene64347	-0.801833	7.47E-49	-1.69E-46
MS.gene059367	MS.gene64347	0.833914	4.06E-56	-1.69E-46
MS.gene059703	MS.gene64347	-0.803796	2.94E-49	-1.69E-46
MS.gene059837	MS.gene64347	0.802087	6.63E-49	-1.69E-46
MS.gene059865	MS.gene64347	0.841621	4.25E-58	-1.69E-46
MS.gene06000	MS.gene64347	0.860343	2.21E-63	-1.69E-46
MS.gene060187	MS.gene64347	-0.804182	2.44E-49	-1.69E-46
MS.gene060593	MS.gene64347	-0.815371	9.57E-52	-1.69E-46
MS.gene060595	MS.gene64347	-0.841389	4.90E-58	-1.69E-46
MS.gene060778	MS.gene64347	0.895855	6.45E-76	-1.69E-46
MS.gene06077	MS.gene64347	0.820209	7.74E-53	-1.69E-46
MS.gene060982	MS.gene64347	0.824819	6.56E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64347.t1	MTR_3g113890	88.372	43	5	0	1	43	1	43	5.60e-20	77.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATAGCAGGGACACATTTCT+TGG	0.264407	3.2:+69434121	None:intergenic
TCTCCATCTTGTTCATGCTT+TGG	0.293177	3.2:-69434818	MS.gene64347:CDS
GGTGGTTTGGTTGGCATAGC+AGG	0.303830	3.2:+69434107	None:intergenic
GGCACTTGCGTCTGCCTCCA+TGG	0.330551	3.2:+69434085	None:intergenic
TGCGTGCCACCAGGAACTTC+TGG	0.331803	3.2:-69434147	MS.gene64347:CDS
TCTTGGTTACCAGAAGTTCC+TGG	0.400394	3.2:+69434138	None:intergenic
GTCTGCCTCCATGGGTGGTT+TGG	0.427775	3.2:+69434094	None:intergenic
GCCTCCATGGGTGGTTTGGT+TGG	0.435015	3.2:+69434098	None:intergenic
CGCCTCTGCCACAGGGCATG+TGG	0.480960	3.2:-69434192	MS.gene64347:intron
GCACTTGCGTCTGCCTCCAT+GGG	0.498020	3.2:+69434086	None:intergenic
CTTGCGTCTGCCTCCATGGG+TGG	0.501890	3.2:+69434089	None:intergenic
TGATGATTCAACTCAATTCA+AGG	0.508747	3.2:+69434063	None:intergenic
CGGTGCAACTGCGTGCCACC+AGG	0.530335	3.2:-69434156	MS.gene64347:CDS
AGCATGAACAAGATGGAGAA+TGG	0.540714	3.2:+69434822	None:intergenic
GTGGTTTGGTTGGCATAGCA+GGG	0.546195	3.2:+69434108	None:intergenic
TGTTCATGCTTTGGACCAGC+CGG	0.555470	3.2:-69434809	MS.gene64347:intron
GATGATTCAACTCAATTCAA+GGG	0.567646	3.2:+69434064	None:intergenic
GGTCCAAAGCATGAACAAGA+TGG	0.576917	3.2:+69434815	None:intergenic
TGAGTTTCTGCGTGTACATG+CGG	0.582718	3.2:+69434673	None:intergenic
CATGTGGAACTTGCTGCAGT+CGG	0.613537	3.2:-69434176	MS.gene64347:CDS
TATGCCAACCAAACCACCCA+TGG	0.640986	3.2:-69434102	MS.gene64347:CDS
TGGTTACCAGAAGTTCCTGG+TGG	0.663688	3.2:+69434141	None:intergenic
GCCAACCAAACCACCCATGG+AGG	0.699460	3.2:-69434099	MS.gene64347:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATATATGAACTAAACTA+AGG	-	chr3.2:69434412-69434431	MS.gene64347:intron	15.0%
!!	TGGAAAAAATATAAAAAAAC+TGG	-	chr3.2:69434366-69434385	MS.gene64347:intron	15.0%
!!!	TTTTAATTATTTGCTTAAAG+TGG	-	chr3.2:69434346-69434365	MS.gene64347:intron	15.0%
!!	ATATAAATTTCAGATTGCAA+TGG	-	chr3.2:69434686-69434705	MS.gene64347:intron	20.0%
!!	GGATAAAAGATTAGTAAAAA+AGG	-	chr3.2:69434526-69434545	MS.gene64347:intron	20.0%
!!!	TATTTTATAAAGAAAACGCT+TGG	+	chr3.2:69434204-69434223	None:intergenic	20.0%
!	TAACGAATATGTATGCTAAT+GGG	-	chr3.2:69434505-69434524	MS.gene64347:intron	25.0%
!	TTAACGAATATGTATGCTAA+TGG	-	chr3.2:69434504-69434523	MS.gene64347:intron	25.0%
!!	TTAATTACATGTGTTTTCGT+AGG	-	chr3.2:69434240-69434259	MS.gene64347:intron	25.0%
!	CTACTTCACACACATATTTT+TGG	+	chr3.2:69434654-69434673	None:intergenic	30.0%
!!	CAACAATAGCTTTGAATTAC+CGG	+	chr3.2:69434151-69434170	None:intergenic	30.0%
	AGCATGAACAAGATGGAGAA+TGG	+	chr3.2:69434119-69434138	None:intergenic	40.0%
	TAAAGAAAACGCTTGGAGCT+TGG	+	chr3.2:69434197-69434216	None:intergenic	40.0%
	TCTCCATCTTGTTCATGCTT+TGG	-	chr3.2:69434120-69434139	MS.gene64347:CDS	40.0%
!	GTTTCTTTTCAGCAGCAGAT+AGG	-	chr3.2:69434289-69434308	MS.gene64347:intron	40.0%
!!	AATAGCTTTGAATTACCGGC+TGG	+	chr3.2:69434147-69434166	None:intergenic	40.0%
	AACCAGACAGAAGCAGTTGA+TGG	+	chr3.2:69434328-69434347	None:intergenic	45.0%
	CATAGCAGGGACACATTTCT+TGG	+	chr3.2:69434820-69434839	None:intergenic	45.0%
	GGTCCAAAGCATGAACAAGA+TGG	+	chr3.2:69434126-69434145	None:intergenic	45.0%
	TCTTGGTTACCAGAAGTTCC+TGG	+	chr3.2:69434803-69434822	None:intergenic	45.0%
	TGAGTTTCTGCGTGTACATG+CGG	+	chr3.2:69434268-69434287	None:intergenic	45.0%
	TTCCATCAACTGCTTCTGTC+TGG	-	chr3.2:69434323-69434342	MS.gene64347:intron	45.0%
!!	CTAGGTGCCGTTTATCATCA+CGG	-	chr3.2:69434720-69434739	MS.gene64347:intron	45.0%
!!	GTAATTAAATTAAATTAAAT+TGG	+	chr3.2:69434228-69434247	None:intergenic	5.0%
	TATGCCAACCAAACCACCCA+TGG	-	chr3.2:69434836-69434855	MS.gene64347:CDS	50.0%
	TGGTTACCAGAAGTTCCTGG+TGG	+	chr3.2:69434800-69434819	None:intergenic	50.0%
!	TGTTCATGCTTTGGACCAGC+CGG	-	chr3.2:69434129-69434148	MS.gene64347:CDS	50.0%
!!	CATGTGGAACTTGCTGCAGT+CGG	-	chr3.2:69434762-69434781	MS.gene64347:intron	50.0%
!!	GTGGTTTGGTTGGCATAGCA+GGG	+	chr3.2:69434833-69434852	None:intergenic	50.0%
	AGCAAGTTCCACATGCCCTG+TGG	+	chr3.2:69434757-69434776	None:intergenic	55.0%
!	GCAATGGAGCATGTGCTGCT+AGG	-	chr3.2:69434702-69434721	MS.gene64347:intron	55.0%
!!	GGTGGTTTGGTTGGCATAGC+AGG	+	chr3.2:69434834-69434853	None:intergenic	55.0%
	GCACTTGCGTCTGCCTCCAT+GGG	+	chr3.2:69434855-69434874	None:intergenic	60.0%
	GCCAACCAAACCACCCATGG+AGG	-	chr3.2:69434839-69434858	MS.gene64347:CDS	60.0%
	GGCGCGGCCGTGATGATAAA+CGG	+	chr3.2:69434730-69434749	None:intergenic	60.0%
	GTCTGCCTCCATGGGTGGTT+TGG	+	chr3.2:69434847-69434866	None:intergenic	60.0%
	TGCGTGCCACCAGGAACTTC+TGG	-	chr3.2:69434791-69434810	MS.gene64347:intron	60.0%
	TTCCACATGCCCTGTGGCAG+AGG	+	chr3.2:69434751-69434770	None:intergenic	60.0%
!!	GCCTCCATGGGTGGTTTGGT+TGG	+	chr3.2:69434843-69434862	None:intergenic	60.0%
	CTTGCGTCTGCCTCCATGGG+TGG	+	chr3.2:69434852-69434871	None:intergenic	65.0%
	GGCACTTGCGTCTGCCTCCA+TGG	+	chr3.2:69434856-69434875	None:intergenic	65.0%
	CATGCCCTGTGGCAGAGGCG+CGG	+	chr3.2:69434746-69434765	None:intergenic	70.0%
	CGCCTCTGCCACAGGGCATG+TGG	-	chr3.2:69434746-69434765	MS.gene64347:intron	70.0%
	CGGTGCAACTGCGTGCCACC+AGG	-	chr3.2:69434782-69434801	MS.gene64347:intron	70.0%
	ACGGCCGCGCCTCTGCCACA+GGG	-	chr3.2:69434739-69434758	MS.gene64347:intron	75.0%
!	CACGGCCGCGCCTCTGCCAC+AGG	-	chr3.2:69434738-69434757	MS.gene64347:intron	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	69434070	69434890	69434070	ID=MS.gene64347
chr3.2	mRNA	69434070	69434890	69434070	ID=MS.gene64347.t1;Parent=MS.gene64347
chr3.2	exon	69434810	69434890	69434810	ID=MS.gene64347.t1.exon1;Parent=MS.gene64347.t1
chr3.2	CDS	69434810	69434890	69434810	ID=cds.MS.gene64347.t1;Parent=MS.gene64347.t1
chr3.2	exon	69434650	69434698	69434650	ID=MS.gene64347.t1.exon2;Parent=MS.gene64347.t1
chr3.2	CDS	69434650	69434698	69434650	ID=cds.MS.gene64347.t1;Parent=MS.gene64347.t1
chr3.2	exon	69434070	69434200	69434070	ID=MS.gene64347.t1.exon3;Parent=MS.gene64347.t1
chr3.2	CDS	69434070	69434200	69434070	ID=cds.MS.gene64347.t1;Parent=MS.gene64347.t1

Gene Sequence

>MS.gene64347

ATGGCTCTCTTTAAGCTTTTAGTTACATCTCTTCTTGTAACTTTTGCCATTCTCCATCTTGTTCATGCTTTGGACCAGCCGGTAATTCAAAGCTATTGTTGCTCTCTTCATTCTTTTATGATTTCCAAGCTCCAAGCGTTTTCTTTATAAAATAACCAATTTAATTTAATTTAATTACATGTGTTTTCGTAGGAACCGCATGTACACGCAGAAACTCAAGTTTCTTTTCAGCAGCAGATAGGTAAAATTACTATTCCATCAACTGCTTCTGTCTGGTTTTAATTATTTGCTTAAAGTGGAAAAAATATAAAAAAACTGGACAGTGAATCAATTAATGAATAAAAAATATATGAACTAAACTAAGGAAGAACACATTCTTAATTATACAATACGAGAATGCAATTATAGCTTATAAAAAAAAAAAATCTAGTTTTTTAACGAATATGTATGCTAATGGGATAAAAGATTAGTAAAAAAGGTTAAATATTATAAAGAAACACAAATCTGTTTCTACTTCACATAAAAATCTTCAAACAAATTAATCTCTAATCAATTTTATCTAACTATCAAATTTATTTTAACCAAAAATATGTGTGTGAAGTAGATGATAAGTTTGATATAAATTTCAGATTGCAATGGAGCATGTGCTGCTAGGTGCCGTTTATCATCACGGCCGCGCCTCTGCCACAGGGCATGTGGAACTTGCTGCAGTCGGTGCAACTGCGTGCCACCAGGAACTTCTGGTAACCAAGAAATGTGTCCCTGCTATGCCAACCAAACCACCCATGGAGGCAGACGCAAGTGCCCTTGAATTGAGTTGA

Protein sequence

>MS.gene64347.t1

MALFKLLVTSLLVTFAILHLVHALDQPEPHVHAETQVSFQQQIGHVELAAVGATACHQELLVTKKCVPAMPTKPPMEADASALELS