AlfalfaGEDB | MS.gene73016

M. sativa cultivar XinJiangDaYe / MS.gene73016

KEGG: K15333
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73016.t1	XP_013457586.1	94.9	99	5	0	1	99	1558	1656	6.10E-47	196.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73016.t1	A0A072UPJ3	94.9	99	5	0	1	99	1558	1656	4.4e-47	196.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene73016	0.837195	6.00E-57	-1.69E-46
MS.gene049711	MS.gene73016	0.812085	5.06E-51	-1.69E-46
MS.gene049821	MS.gene73016	0.805032	1.62E-49	-1.69E-46
MS.gene049824	MS.gene73016	0.85379	1.89E-61	-1.69E-46
MS.gene049825	MS.gene73016	0.851254	1.00E-60	-1.69E-46
MS.gene049850	MS.gene73016	0.824382	8.32E-54	-1.69E-46
MS.gene049873	MS.gene73016	0.837089	6.39E-57	-1.69E-46
MS.gene050559	MS.gene73016	0.827759	1.31E-54	-1.69E-46
MS.gene050561	MS.gene73016	0.819835	9.43E-53	-1.69E-46
MS.gene050562	MS.gene73016	0.814875	1.23E-51	-1.69E-46
MS.gene051021	MS.gene73016	0.802938	4.42E-49	-1.69E-46
MS.gene051247	MS.gene73016	0.821284	4.38E-53	-1.69E-46
MS.gene051279	MS.gene73016	0.837794	4.21E-57	-1.69E-46
MS.gene051594	MS.gene73016	0.819668	1.03E-52	-1.69E-46
MS.gene051700	MS.gene73016	0.807426	5.09E-50	-1.69E-46
MS.gene051719	MS.gene73016	0.846475	2.12E-59	-1.69E-46
MS.gene051720	MS.gene73016	0.851235	1.01E-60	-1.69E-46
MS.gene051744	MS.gene73016	0.860754	1.66E-63	-1.69E-46
MS.gene051957	MS.gene73016	0.808816	2.57E-50	-1.69E-46
MS.gene052463	MS.gene73016	0.808604	2.85E-50	-1.69E-46
MS.gene052519	MS.gene73016	0.829634	4.61E-55	-1.69E-46
MS.gene052582	MS.gene73016	0.829396	5.27E-55	-1.69E-46
MS.gene052584	MS.gene73016	0.843762	1.15E-58	-1.69E-46
MS.gene052773	MS.gene73016	0.809665	1.69E-50	-1.69E-46
MS.gene053079	MS.gene73016	0.809491	1.84E-50	-1.69E-46
MS.gene053122	MS.gene73016	0.803444	3.48E-49	-1.69E-46
MS.gene053144	MS.gene73016	0.800822	1.20E-48	-1.69E-46
MS.gene053226	MS.gene73016	0.859559	3.80E-63	-1.69E-46
MS.gene053296	MS.gene73016	0.826904	2.10E-54	-1.69E-46
MS.gene053581	MS.gene73016	0.809556	1.79E-50	-1.69E-46
MS.gene053597	MS.gene73016	0.800681	1.29E-48	-1.69E-46
MS.gene053599	MS.gene73016	0.832572	8.76E-56	-1.69E-46
MS.gene05364	MS.gene73016	0.800075	1.71E-48	-1.69E-46
MS.gene053678	MS.gene73016	0.80268	5.00E-49	-1.69E-46
MS.gene05423	MS.gene73016	0.828425	9.06E-55	-1.69E-46
MS.gene054576	MS.gene73016	0.810847	9.40E-51	-1.69E-46
MS.gene054584	MS.gene73016	0.803264	3.79E-49	-1.69E-46
MS.gene054651	MS.gene73016	0.84548	3.96E-59	-1.69E-46
MS.gene054775	MS.gene73016	0.824047	9.97E-54	-1.69E-46
MS.gene054967	MS.gene73016	0.81846	1.94E-52	-1.69E-46
MS.gene054991	MS.gene73016	0.812607	3.89E-51	-1.69E-46
MS.gene055465	MS.gene73016	0.829363	5.37E-55	-1.69E-46
MS.gene055466	MS.gene73016	0.848844	4.73E-60	-1.69E-46
MS.gene055468	MS.gene73016	0.819063	1.41E-52	-1.69E-46
MS.gene055513	MS.gene73016	0.807465	4.99E-50	-1.69E-46
MS.gene055580	MS.gene73016	-0.812077	5.08E-51	-1.69E-46
MS.gene055778	MS.gene73016	0.834916	2.27E-56	-1.69E-46
MS.gene055808	MS.gene73016	0.814419	1.56E-51	-1.69E-46
MS.gene055818	MS.gene73016	0.80604	9.98E-50	-1.69E-46
MS.gene056050	MS.gene73016	0.812236	4.69E-51	-1.69E-46
MS.gene056174	MS.gene73016	0.81911	1.38E-52	-1.69E-46
MS.gene056215	MS.gene73016	0.815908	7.27E-52	-1.69E-46
MS.gene056366	MS.gene73016	0.841367	4.96E-58	-1.69E-46
MS.gene05686	MS.gene73016	0.82306	1.70E-53	-1.69E-46
MS.gene057102	MS.gene73016	0.802568	5.27E-49	-1.69E-46
MS.gene057444	MS.gene73016	0.812622	3.87E-51	-1.69E-46
MS.gene058037	MS.gene73016	0.825171	5.42E-54	-1.69E-46
MS.gene058625	MS.gene73016	0.845883	3.08E-59	-1.69E-46
MS.gene059015	MS.gene73016	0.806256	8.98E-50	-1.69E-46
MS.gene059084	MS.gene73016	0.818524	1.87E-52	-1.69E-46
MS.gene05954	MS.gene73016	0.833541	5.03E-56	-1.69E-46
MS.gene059625	MS.gene73016	0.850115	2.09E-60	-1.69E-46
MS.gene060429	MS.gene73016	0.811575	6.54E-51	-1.69E-46
MS.gene060519	MS.gene73016	0.812104	5.01E-51	-1.69E-46
MS.gene060521	MS.gene73016	0.802419	5.66E-49	-1.69E-46
MS.gene060587	MS.gene73016	0.826743	2.29E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73016.t1	MTR_4g099300	94.949	99	5	0	1	99	1558	1656	1.06e-62	205

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73016.t1	AT4G17610	48.485	99	50	1	1	98	1541	1639	2.46e-23	93.2
MS.gene73016.t1	AT4G17610	48.485	99	50	1	1	98	1542	1640	2.46e-23	93.2
MS.gene73016.t1	AT4G17610	48.485	99	50	1	1	98	1549	1647	2.48e-23	93.2

Find 16 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGTGAAGCAACCTTGTTTA+AGG	0.203858	8.1:-22994642	MS.gene73016:CDS
GGGGACAATGATGCAGGATT+AGG	0.408382	8.1:-22994569	MS.gene73016:CDS
ATTGTCCCCTGCATCATGTT+TGG	0.408627	8.1:+22994583	None:intergenic
CAAAGGACGTGGTAACTATA+AGG	0.410377	8.1:-22994718	MS.gene73016:CDS
AGTGAGACTCTAAAATTCAA+TGG	0.491970	8.1:-22994695	MS.gene73016:CDS
ATGTTCGATGGCAAAGGACG+TGG	0.495024	8.1:-22994729	MS.gene73016:CDS
ACATTTACCAAACATGATGC+AGG	0.525324	8.1:-22994590	MS.gene73016:CDS
GATGCAGGGGACAATGATGC+AGG	0.572775	8.1:-22994575	MS.gene73016:CDS
ATGTGTTCCAACGTGTCTCA+TGG	0.575870	8.1:-22994882	MS.gene73016:CDS
CATTTACCAAACATGATGCA+GGG	0.596237	8.1:-22994589	MS.gene73016:CDS
ATTCAATGGAGATAATTGCA+TGG	0.609541	8.1:-22994681	MS.gene73016:CDS
CTTACGATGTTCGATGGCAA+AGG	0.614421	8.1:-22994735	MS.gene73016:CDS
AGAAGACTTACGATGTTCGA+TGG	0.618099	8.1:-22994741	MS.gene73016:CDS
ACATGCTCCATGAGACACGT+TGG	0.655498	8.1:+22994875	None:intergenic
TGAGGACATATCCTTAAACA+AGG	0.667721	8.1:+22994631	None:intergenic
ATTTACCAAACATGATGCAG+GGG	0.675007	8.1:-22994588	MS.gene73016:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAGCATAATTCTTTAAATA+GGG	+	chr8.1:22994617-22994636	None:intergenic	15.0%
!!	TTTAGCATAATTCTTTAAAT+AGG	+	chr8.1:22994618-22994637	None:intergenic	15.0%
!	AATTGCATGGAAAAATTATC+TGG	-	chr8.1:22994762-22994781	MS.gene73016:intron	25.0%
!!	TGGTAAATGTTATCTTTTTC+TGG	+	chr8.1:22994830-22994849	None:intergenic	25.0%
	ATTCAATGGAGATAATTGCA+TGG	-	chr8.1:22994749-22994768	MS.gene73016:CDS	30.0%
	TGATGAAACTTACCGAAAAA+TGG	-	chr8.1:22994893-22994912	MS.gene73016:CDS	30.0%
!	TTAAATAGGGAAGTATGACA+AGG	+	chr8.1:22994604-22994623	None:intergenic	30.0%
!!	AGTGAGACTCTAAAATTCAA+TGG	-	chr8.1:22994735-22994754	MS.gene73016:CDS	30.0%
!!	TATCTTTTTCTGGAAATCTG+AGG	+	chr8.1:22994820-22994839	None:intergenic	30.0%
	ACATTTACCAAACATGATGC+AGG	-	chr8.1:22994840-22994859	MS.gene73016:intron	35.0%
	ATTTACCAAACATGATGCAG+GGG	-	chr8.1:22994842-22994861	MS.gene73016:intron	35.0%
	CATTTACCAAACATGATGCA+GGG	-	chr8.1:22994841-22994860	MS.gene73016:intron	35.0%
	TGAGGACATATCCTTAAACA+AGG	+	chr8.1:22994802-22994821	None:intergenic	35.0%
	TGCATGGAAAAATTATCTGG+TGG	-	chr8.1:22994765-22994784	MS.gene73016:intron	35.0%
!	TTTTTATCCCCAATCATTGC+AGG	-	chr8.1:22994659-22994678	MS.gene73016:CDS	35.0%
	AGAAGACTTACGATGTTCGA+TGG	-	chr8.1:22994689-22994708	MS.gene73016:CDS	40.0%
	CAAAGGACGTGGTAACTATA+AGG	-	chr8.1:22994712-22994731	MS.gene73016:CDS	40.0%
	GAAACTTACCGAAAAATGGC+TGG	-	chr8.1:22994897-22994916	MS.gene73016:CDS	40.0%
	TCTAGCATCCTGCAATGATT+GGG	+	chr8.1:22994670-22994689	None:intergenic	40.0%
!	CAGTGAAGCAACCTTGTTTA+AGG	-	chr8.1:22994788-22994807	MS.gene73016:intron	40.0%
	ATGTGTTCCAACGTGTCTCA+TGG	-	chr8.1:22994548-22994567	MS.gene73016:CDS	45.0%
	ATTGTCCCCTGCATCATGTT+TGG	+	chr8.1:22994850-22994869	None:intergenic	45.0%
	CTAGCATCCTGCAATGATTG+GGG	+	chr8.1:22994669-22994688	None:intergenic	45.0%
	CTCTAGCATCCTGCAATGAT+TGG	+	chr8.1:22994671-22994690	None:intergenic	45.0%
	CTTACGATGTTCGATGGCAA+AGG	-	chr8.1:22994695-22994714	MS.gene73016:CDS	45.0%
	ACATGCTCCATGAGACACGT+TGG	+	chr8.1:22994558-22994577	None:intergenic	50.0%
	ATGTTCGATGGCAAAGGACG+TGG	-	chr8.1:22994701-22994720	MS.gene73016:CDS	50.0%
	GGGGACAATGATGCAGGATT+AGG	-	chr8.1:22994861-22994880	MS.gene73016:CDS	50.0%
	GATGCAGGGGACAATGATGC+AGG	-	chr8.1:22994855-22994874	MS.gene73016:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	22994534	22994918	22994534	ID=MS.gene73016
chr8.1	mRNA	22994534	22994918	22994534	ID=MS.gene73016.t1;Parent=MS.gene73016
chr8.1	exon	22994859	22994918	22994859	ID=MS.gene73016.t1.exon1;Parent=MS.gene73016.t1
chr8.1	CDS	22994859	22994918	22994859	ID=cds.MS.gene73016.t1;Parent=MS.gene73016.t1
chr8.1	exon	22994534	22994771	22994534	ID=MS.gene73016.t1.exon2;Parent=MS.gene73016.t1
chr8.1	CDS	22994534	22994771	22994534	ID=cds.MS.gene73016.t1;Parent=MS.gene73016.t1

Gene Sequence

>MS.gene73016

GAAAACAATTTTGAATGTGTTCCAACGTGTCTCATGGAGCATGTTCTCAAATTTTTAAATGTAAGTTCCTTGTCATACTTCCCTATTTAAAGAATTATGCTAAATTTTTTGTTTCATTCTGTGAGTTTTTATCCCCAATCATTGCAGGATGCTAGAGAAGACTTACGATGTTCGATGGCAAAGGACGTGGTAACTATAAGGAGTGAGACTCTAAAATTCAATGGAGATAATTGCATGGAAAAATTATCTGGTGGCAGTGAAGCAACCTTGTTTAAGGATATGTCCTCAGATTTCCAGAAAAAGATAACATTTACCAAACATGATGCAGGGGACAATGATGCAGGATTAGGTTGCGACGATGATGAAACTTACCGAAAAATGGCTG

Protein sequence

>MS.gene73016.t1

ENNFECVPTCLMEHVLKFLNDAREDLRCSMAKDVVTIRSETLKFNGDNCMEKLSGGSEATLFKDMSSDFQKKITFTKHDAGDNDAGLGCDDDETYRKMA