Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76344.t1 | RHN45016.1 | 89.1 | 734 | 59 | 3 | 1 | 718 | 1 | 729 | 3.40E-179 | 638.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76344.t1 | Q9Y222 | 25.8 | 256 | 173 | 7 | 439 | 689 | 156 | 399 | 4.8e-17 | 91.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76344.t1 | A0A396GV57 | 89.1 | 734 | 59 | 3 | 1 | 718 | 1 | 729 | 2.4e-179 | 638.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene76344.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene76344 | 0.844136 | 9.11E-59 | -1.69E-46 |
| MS.gene049159 | MS.gene76344 | 0.840394 | 8.93E-58 | -1.69E-46 |
| MS.gene049368 | MS.gene76344 | 0.839006 | 2.05E-57 | -1.69E-46 |
| MS.gene049411 | MS.gene76344 | 0.801185 | 1.01E-48 | -1.69E-46 |
| MS.gene050384 | MS.gene76344 | 0.811545 | 6.64E-51 | -1.69E-46 |
| MS.gene050386 | MS.gene76344 | 0.81843 | 1.97E-52 | -1.69E-46 |
| MS.gene050551 | MS.gene76344 | 0.816807 | 4.57E-52 | -1.69E-46 |
| MS.gene050654 | MS.gene76344 | 0.804017 | 2.64E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene76344 | 0.869374 | 3.25E-66 | -1.69E-46 |
| MS.gene050737 | MS.gene76344 | 0.822006 | 2.98E-53 | -1.69E-46 |
| MS.gene050815 | MS.gene76344 | 0.80989 | 1.51E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene76344 | 0.814425 | 1.55E-51 | -1.69E-46 |
| MS.gene050941 | MS.gene76344 | 0.85666 | 2.77E-62 | -1.69E-46 |
| MS.gene051003 | MS.gene76344 | 0.827982 | 1.16E-54 | -1.69E-46 |
| MS.gene051328 | MS.gene76344 | 0.846565 | 2.00E-59 | -1.69E-46 |
| MS.gene051381 | MS.gene76344 | 0.800039 | 1.74E-48 | -1.69E-46 |
| MS.gene051452 | MS.gene76344 | 0.804642 | 1.96E-49 | -1.69E-46 |
| MS.gene051975 | MS.gene76344 | 0.808381 | 3.18E-50 | -1.69E-46 |
| MS.gene052307 | MS.gene76344 | 0.809449 | 1.88E-50 | -1.69E-46 |
| MS.gene052342 | MS.gene76344 | 0.814839 | 1.26E-51 | -1.69E-46 |
| MS.gene052434 | MS.gene76344 | 0.822789 | 1.96E-53 | -1.69E-46 |
| MS.gene052516 | MS.gene76344 | 0.83834 | 3.05E-57 | -1.69E-46 |
| MS.gene052518 | MS.gene76344 | 0.821299 | 4.35E-53 | -1.69E-46 |
| MS.gene052863 | MS.gene76344 | 0.855485 | 6.11E-62 | -1.69E-46 |
| MS.gene052896 | MS.gene76344 | 0.85015 | 2.05E-60 | -1.69E-46 |
| MS.gene053008 | MS.gene76344 | 0.865389 | 6.12E-65 | -1.69E-46 |
| MS.gene053381 | MS.gene76344 | 0.842315 | 2.79E-58 | -1.69E-46 |
| MS.gene054472 | MS.gene76344 | 0.802235 | 6.18E-49 | -1.69E-46 |
| MS.gene05458 | MS.gene76344 | 0.822286 | 2.57E-53 | -1.69E-46 |
| MS.gene054823 | MS.gene76344 | 0.824427 | 8.12E-54 | -1.69E-46 |
| MS.gene05517 | MS.gene76344 | 0.820003 | 8.63E-53 | -1.69E-46 |
| MS.gene055310 | MS.gene76344 | 0.807952 | 3.93E-50 | -1.69E-46 |
| MS.gene055311 | MS.gene76344 | 0.825131 | 5.54E-54 | -1.69E-46 |
| MS.gene055313 | MS.gene76344 | 0.812388 | 4.35E-51 | -1.69E-46 |
| MS.gene055466 | MS.gene76344 | 0.806189 | 9.28E-50 | -1.69E-46 |
| MS.gene055468 | MS.gene76344 | 0.812791 | 3.55E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene76344 | 0.841422 | 4.80E-58 | -1.69E-46 |
| MS.gene055979 | MS.gene76344 | 0.837467 | 5.11E-57 | -1.69E-46 |
| MS.gene056181 | MS.gene76344 | 0.828781 | 7.43E-55 | -1.69E-46 |
| MS.gene056308 | MS.gene76344 | 0.807129 | 5.88E-50 | -1.69E-46 |
| MS.gene056787 | MS.gene76344 | 0.807458 | 5.01E-50 | -1.69E-46 |
| MS.gene056831 | MS.gene76344 | 0.825968 | 3.51E-54 | -1.69E-46 |
| MS.gene057057 | MS.gene76344 | 0.805742 | 1.15E-49 | -1.69E-46 |
| MS.gene057140 | MS.gene76344 | 0.803283 | 3.75E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene76344 | 0.814374 | 1.59E-51 | -1.69E-46 |
| MS.gene058292 | MS.gene76344 | 0.801047 | 1.08E-48 | -1.69E-46 |
| MS.gene058646 | MS.gene76344 | 0.841546 | 4.45E-58 | -1.69E-46 |
| MS.gene058654 | MS.gene76344 | 0.817491 | 3.21E-52 | -1.69E-46 |
| MS.gene058783 | MS.gene76344 | 0.803158 | 3.98E-49 | -1.69E-46 |
| MS.gene058906 | MS.gene76344 | 0.801525 | 8.64E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene76344 | 0.804804 | 1.81E-49 | -1.69E-46 |
| MS.gene059283 | MS.gene76344 | 0.824619 | 7.32E-54 | -1.69E-46 |
| MS.gene059842 | MS.gene76344 | 0.854699 | 1.03E-61 | -1.69E-46 |
| MS.gene06010 | MS.gene76344 | 0.816489 | 5.39E-52 | -1.69E-46 |
| MS.gene060296 | MS.gene76344 | 0.807739 | 4.36E-50 | -1.69E-46 |
| MS.gene060637 | MS.gene76344 | 0.800032 | 1.74E-48 | -1.69E-46 |
| MS.gene060803 | MS.gene76344 | 0.811429 | 7.03E-51 | -1.69E-46 |
| MS.gene060870 | MS.gene76344 | 0.807288 | 5.44E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene76344 | 0.807812 | 4.21E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76344.t1 | MTR_8g077990 | 87.755 | 735 | 31 | 5 | 1 | 718 | 1 | 693 | 0.0 | 1115 |
| MS.gene76344.t1 | MTR_1g076600 | 95.050 | 101 | 5 | 0 | 618 | 718 | 55 | 155 | 1.86e-66 | 216 |
| MS.gene76344.t1 | MTR_7g029435 | 93.478 | 92 | 6 | 0 | 627 | 718 | 1 | 92 | 5.51e-57 | 189 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76344.t1 | AT5G41020 | 55.124 | 283 | 126 | 1 | 433 | 715 | 302 | 583 | 1.76e-112 | 352 |
Find 125 sgRNAs with CRISPR-Local
Find 142 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATAAATTCAAGCTCTTC+AGG | 0.254261 | 8.1:+29535893 | None:intergenic |
| ATCATCATCTTCACATTCAT+TGG | 0.267885 | 8.1:+29536624 | None:intergenic |
| AACAAATGATCCACCATATT+GGG | 0.286872 | 8.1:-29535409 | MS.gene76344:CDS |
| ACATTGCTTTCGGACATATA+TGG | 0.292466 | 8.1:+29537366 | None:intergenic |
| TTGATCTTCTCATCTTCTTC+GGG | 0.322099 | 8.1:+29536112 | None:intergenic |
| TTTGATCTTCTCATCTTCTT+CGG | 0.324153 | 8.1:+29536111 | None:intergenic |
| GAACAAATGATCCACCATAT+TGG | 0.329038 | 8.1:-29535410 | MS.gene76344:CDS |
| CTATATAGATTCTCATGGTT+TGG | 0.339430 | 8.1:-29536074 | MS.gene76344:CDS |
| TATATAGATTCTCATGGTTT+GGG | 0.352407 | 8.1:-29536073 | MS.gene76344:CDS |
| TCTCTATCTTTCAACAAGTT+GGG | 0.365789 | 8.1:+29535329 | None:intergenic |
| CTCTCTATCTTTCAACAAGT+TGG | 0.365952 | 8.1:+29535328 | None:intergenic |
| CAGATCAGTTACATTGCTTT+CGG | 0.367004 | 8.1:+29537356 | None:intergenic |
| TTATGTGTGCTTTCTTCACT+TGG | 0.367181 | 8.1:+29536859 | None:intergenic |
| TAGCCCTCTCAAGCAGATCT+AGG | 0.372193 | 8.1:+29535708 | None:intergenic |
| GATAGACGCTTTATATGCTT+TGG | 0.375237 | 8.1:-29535510 | MS.gene76344:CDS |
| ACTCCTAACTCTGCACATAA+TGG | 0.384709 | 8.1:-29536223 | MS.gene76344:CDS |
| GTTCCATTATGTGCAGAGTT+AGG | 0.391649 | 8.1:+29536220 | None:intergenic |
| CTGATCTGCCTTTGATCAAA+AGG | 0.395638 | 8.1:-29537341 | MS.gene76344:CDS |
| TGGCGGCATGCTCCCCAATA+TGG | 0.396320 | 8.1:+29535396 | None:intergenic |
| GGGAGACGAAGGTCTTGATA+TGG | 0.410993 | 8.1:-29536053 | MS.gene76344:CDS |
| TTCCAACAGCCGCTAACCTC+AGG | 0.416232 | 8.1:+29536010 | None:intergenic |
| AGAAGCAATGAAGGTGATAA+TGG | 0.417384 | 8.1:-29537105 | MS.gene76344:CDS |
| GTTTCATTATGTGCACAGTT+AGG | 0.421260 | 8.1:+29536244 | None:intergenic |
| GTTTCATTATGTGCACAGTT+AGG | 0.421260 | 8.1:+29536376 | None:intergenic |
| GAGAGCAGTAGCTGACGCTT+TGG | 0.427556 | 8.1:-29535840 | MS.gene76344:CDS |
| TGCTCATACTTTGTTAGAAA+AGG | 0.429545 | 8.1:-29535936 | MS.gene76344:CDS |
| GTTGCGAGATAATATTTGTT+GGG | 0.434220 | 8.1:-29535654 | MS.gene76344:CDS |
| TCATCATCTTCACATTCATT+GGG | 0.442787 | 8.1:+29536625 | None:intergenic |
| AGAGCAGTAGCTGACGCTTT+GGG | 0.446113 | 8.1:-29535839 | MS.gene76344:CDS |
| GCTGCATGGAGGAGGTAGAC+TGG | 0.446516 | 8.1:-29535481 | MS.gene76344:CDS |
| GTCACTGATGTTAATGATCA+AGG | 0.447087 | 8.1:-29536316 | MS.gene76344:CDS |
| CTAAACGCAAACAAGTAACT+TGG | 0.447239 | 8.1:-29536195 | MS.gene76344:CDS |
| TATTCTTCTTCGTCTTGCCT+TGG | 0.449662 | 8.1:+29536548 | None:intergenic |
| CGCCAAGTCATTCATTGAGC+AGG | 0.451714 | 8.1:-29535378 | MS.gene76344:CDS |
| CATTGGTTGCTAGTGGTGAT+TGG | 0.452021 | 8.1:-29535556 | MS.gene76344:CDS |
| AATATTTGTTGGGAGGCAAT+TGG | 0.463198 | 8.1:-29535644 | MS.gene76344:CDS |
| GTCACTGAAGTTAATGATCA+AGG | 0.467385 | 8.1:-29536448 | MS.gene76344:CDS |
| CGTCGATACACACTCTCATA+AGG | 0.471062 | 8.1:+29535959 | None:intergenic |
| ACACTCTCATAAGGCCTCTC+AGG | 0.477449 | 8.1:+29535968 | None:intergenic |
| AAGAAGCACAAACTAATTGA+AGG | 0.478439 | 8.1:-29536778 | MS.gene76344:CDS |
| ACTAGCTCCATGTTGCTCAT+AGG | 0.479373 | 8.1:+29535865 | None:intergenic |
| TGCTTTGGATGCTTGCTGCA+TGG | 0.479442 | 8.1:-29535495 | MS.gene76344:CDS |
| AAAAGGAGGGAAGGTGTAAG+TGG | 0.481580 | 8.1:-29535919 | MS.gene76344:CDS |
| TAGAAGCAATGGAGGTGAAG+AGG | 0.481860 | 8.1:-29536728 | MS.gene76344:CDS |
| ACGGCTTATTGCGTGGGAAA+AGG | 0.484774 | 8.1:-29536141 | MS.gene76344:CDS |
| TGTTGCGAGATAATATTTGT+TGG | 0.489321 | 8.1:-29535655 | MS.gene76344:CDS |
| GGATGCTTGCTGCATGGAGG+AGG | 0.492182 | 8.1:-29535489 | MS.gene76344:CDS |
| ACCCTGAGGTTAGCGGCTGT+TGG | 0.493985 | 8.1:-29536012 | MS.gene76344:CDS |
| AAGTGAAGAAAGCACACATA+AGG | 0.495960 | 8.1:-29536857 | MS.gene76344:CDS |
| AAGTGAAGAAAGTAAACATA+AGG | 0.498172 | 8.1:-29536665 | MS.gene76344:CDS |
| TGAAGGTGATAATGGCGAGC+AGG | 0.499793 | 8.1:-29537097 | MS.gene76344:CDS |
| GAGTGACGAAAGCAAACATA+AGG | 0.503590 | 8.1:-29537046 | MS.gene76344:CDS |
| CTCTATCTTTCAACAAGTTG+GGG | 0.504361 | 8.1:+29535330 | None:intergenic |
| AGAAACTAATTGAGGAAGGT+GGG | 0.510841 | 8.1:-29536579 | MS.gene76344:CDS |
| TGTAATGACTTTAGAAGCAA+TGG | 0.511180 | 8.1:-29536739 | MS.gene76344:CDS |
| AAAAGCAATAAAAGTGAAGA+TGG | 0.511576 | 8.1:-29537297 | MS.gene76344:CDS |
| CTGCTCAATGAATGACTTGG+CGG | 0.516982 | 8.1:+29535379 | None:intergenic |
| AAGAAACTAATTGAGGAAGG+TGG | 0.519932 | 8.1:-29536580 | MS.gene76344:CDS |
| AATCTACTTGAGCATAGGTC+TGG | 0.523977 | 8.1:-29535455 | MS.gene76344:CDS |
| CTGCATGGAGGAGGTAGACT+GGG | 0.526890 | 8.1:-29535480 | MS.gene76344:CDS |
| TCAGCATCACCTTCATTGCT+AGG | 0.528214 | 8.1:+29536352 | None:intergenic |
| TCATACTTTGTTAGAAAAGG+AGG | 0.529507 | 8.1:-29535933 | MS.gene76344:CDS |
| AATGAAGAAGAAACTAATTG+AGG | 0.530342 | 8.1:-29536587 | MS.gene76344:CDS |
| TTTGACTATATAGATTCTCA+TGG | 0.532297 | 8.1:-29536079 | MS.gene76344:CDS |
| AAGAAGCGGAAGCTAATTGA+AGG | 0.534553 | 8.1:-29537156 | MS.gene76344:CDS |
| CATATATGGATCGCCTCCAT+TGG | 0.534788 | 8.1:+29537380 | None:intergenic |
| GGAGGCAATTGGTCACAAGT+TGG | 0.536501 | 8.1:-29535633 | MS.gene76344:CDS |
| AAAAGTGAAGATGGCGAGCA+AGG | 0.538269 | 8.1:-29537288 | MS.gene76344:CDS |
| ATAGGTCTGGTGATGTGTGC+AGG | 0.541652 | 8.1:-29535442 | MS.gene76344:CDS |
| ATGAGCAACATGGAGCTAGT+TGG | 0.543870 | 8.1:-29535862 | MS.gene76344:CDS |
| AATCACAGGTTTGTTGTCAA+AGG | 0.550299 | 8.1:+29535304 | None:intergenic |
| TTTGGGTAAAAGTCGAGCTC+AGG | 0.550583 | 8.1:-29535822 | MS.gene76344:CDS |
| AGTAACTTGGGCTGATGAAG+TGG | 0.552578 | 8.1:-29536182 | MS.gene76344:CDS |
| AAGAAGAAACTAATTGAGGA+AGG | 0.552685 | 8.1:-29536583 | MS.gene76344:CDS |
| AATGAAACTCCTAGCAATGA+AGG | 0.554019 | 8.1:-29536361 | MS.gene76344:CDS |
| AAGAGAACTGAAGGCGAAGA+GGG | 0.556027 | 8.1:-29537408 | MS.gene76344:CDS |
| GACGGCTTTAGAAGCAATGA+AGG | 0.559488 | 8.1:-29537114 | MS.gene76344:CDS |
| TGTTGTGACGGCTTATTGCG+TGG | 0.561335 | 8.1:-29536148 | MS.gene76344:CDS |
| TGAGCAATTAACATCAACAT+TGG | 0.568371 | 8.1:-29535573 | MS.gene76344:CDS |
| TAAACCTAGATCTGCTTGAG+AGG | 0.570723 | 8.1:-29535712 | MS.gene76344:CDS |
| AGAAGCAATGGAGGTGAAGA+GGG | 0.572295 | 8.1:-29536727 | MS.gene76344:CDS |
| TAGAAACCTCAACAAATCAC+AGG | 0.579568 | 8.1:+29535290 | None:intergenic |
| GAAGGTGATAATGGCGAGCA+GGG | 0.580967 | 8.1:-29537096 | MS.gene76344:CDS |
| CATACTTTGTTAGAAAAGGA+GGG | 0.581182 | 8.1:-29535932 | MS.gene76344:CDS |
| ACATCAACATTGGTTGCTAG+TGG | 0.583463 | 8.1:-29535563 | MS.gene76344:CDS |
| AACAAACCTGTGATTTGTTG+AGG | 0.586181 | 8.1:-29535296 | None:intergenic |
| GCGAGATAATATTTGTTGGG+AGG | 0.587211 | 8.1:-29535651 | MS.gene76344:CDS |
| CATATAAAGCGTCTATCAAG+CGG | 0.589919 | 8.1:+29535516 | None:intergenic |
| GGCGAAGAGGGTGAAACCAA+TGG | 0.590103 | 8.1:-29537396 | MS.gene76344:CDS |
| GAGGAGAAGTTATGTTGTGA+CGG | 0.591020 | 8.1:-29536160 | MS.gene76344:CDS |
| AAATTACACCCTGAGGTTAG+CGG | 0.591381 | 8.1:-29536019 | MS.gene76344:CDS |
| CTTTGTTAGAAAAGGAGGGA+AGG | 0.592917 | 8.1:-29535928 | MS.gene76344:CDS |
| CGGCATGCTCCCCAATATGG+TGG | 0.593209 | 8.1:+29535399 | None:intergenic |
| AGAAGCAATGATGATGAAGA+TGG | 0.594055 | 8.1:-29536919 | MS.gene76344:CDS |
| AAACCTAGATCTGCTTGAGA+GGG | 0.602034 | 8.1:-29535711 | MS.gene76344:CDS |
| TCCAACAGCCGCTAACCTCA+GGG | 0.602654 | 8.1:+29536011 | None:intergenic |
| TAAACGCAAACAAGTAACTT+GGG | 0.603412 | 8.1:-29536194 | MS.gene76344:CDS |
| CAACTTGTTGAAAGATAGAG+AGG | 0.613256 | 8.1:-29535327 | MS.gene76344:CDS |
| AACTTGGGCTGATGAAGTGG+AGG | 0.617904 | 8.1:-29536179 | MS.gene76344:CDS |
| GGGACAATCTACTTGAGCAT+AGG | 0.618965 | 8.1:-29535460 | MS.gene76344:CDS |
| CAAGAGAACTGAAGGCGAAG+AGG | 0.619279 | 8.1:-29537409 | MS.gene76344:CDS |
| GAAGGAAACACACTTGAGGA+AGG | 0.622295 | 8.1:-29537138 | MS.gene76344:CDS |
| ACAAATGATCCACCATATTG+GGG | 0.622830 | 8.1:-29535408 | MS.gene76344:CDS |
| TCTCATGGTTTGGGAGACGA+AGG | 0.623440 | 8.1:-29536064 | MS.gene76344:CDS |
| AACCTGCTCAATGAATGACT+TGG | 0.625939 | 8.1:+29535376 | None:intergenic |
| GTGATGTGTGCAGGAAACGA+TGG | 0.634053 | 8.1:-29535433 | MS.gene76344:CDS |
| GAAGGTGGGAATCGTGACCA+AGG | 0.636683 | 8.1:-29536565 | MS.gene76344:CDS |
| CTGCCTTTGATCAAAAGGAA+TGG | 0.637174 | 8.1:-29537336 | MS.gene76344:CDS |
| GAAAAGACCTATGAGCAACA+TGG | 0.638568 | 8.1:-29535872 | MS.gene76344:CDS |
| GTTGTGACGGCTTATTGCGT+GGG | 0.642851 | 8.1:-29536147 | MS.gene76344:CDS |
| TTTGGATGCTTGCTGCATGG+AGG | 0.643384 | 8.1:-29535492 | MS.gene76344:CDS |
| TGATGTGTGCAGGAAACGAT+GGG | 0.644218 | 8.1:-29535432 | MS.gene76344:CDS |
| AATGAATGTGAAGATGATGA+TGG | 0.645642 | 8.1:-29536622 | MS.gene76344:CDS |
| AATTGAAGGAAACACACTGA+AGG | 0.653637 | 8.1:-29536956 | MS.gene76344:CDS |
| AATGACTTTAGAAGCAATGG+AGG | 0.658645 | 8.1:-29536736 | MS.gene76344:CDS |
| AACACACTTGAGGAAGGTGA+CGG | 0.661228 | 8.1:-29537132 | MS.gene76344:CDS |
| AAATGTCAAATTACACCCTG+AGG | 0.666420 | 8.1:-29536026 | MS.gene76344:CDS |
| AAAAGGACATTGGTCCCAAG+AGG | 0.666914 | 8.1:-29535759 | MS.gene76344:CDS |
| AATTGAAGGAAACACACTTG+AGG | 0.669341 | 8.1:-29537142 | MS.gene76344:CDS |
| TGTGAAGATGATGATGGCCA+AGG | 0.683186 | 8.1:-29536616 | MS.gene76344:CDS |
| GAGCTCAGGTAAAAGATGCG+TGG | 0.687675 | 8.1:-29535808 | MS.gene76344:CDS |
| GAAGAGGGTGAAACCAATGG+AGG | 0.700449 | 8.1:-29537393 | MS.gene76344:CDS |
| GAATGTGAAGCTGATGACCA+AGG | 0.711673 | 8.1:-29537000 | MS.gene76344:CDS |
| AATGAATGTGAAGATGACCA+AGG | 0.719006 | 8.1:-29536814 | MS.gene76344:CDS |
| GAATGTGAAGATGATGACCA+AGG | 0.731540 | 8.1:-29537192 | MS.gene76344:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATACCAAAGCAAAAAA+AGG | - | chr8.1:29536954-29536973 | MS.gene76344:CDS | 20.0% |
| !!! | AATGATTTTAATAGCAAAGA+AGG | - | chr8.1:29536237-29536256 | MS.gene76344:CDS | 20.0% |
| ! | AAAAGCAATAAAAGTGAAGA+TGG | - | chr8.1:29535433-29535452 | MS.gene76344:CDS | 25.0% |
| ! | AAGTGAAGAAAGTAAACATA+AGG | - | chr8.1:29536065-29536084 | MS.gene76344:CDS | 25.0% |
| ! | AATGAAGAAGAAACTAATTG+AGG | - | chr8.1:29536143-29536162 | MS.gene76344:CDS | 25.0% |
| ! | ATGAAAAAGAAGCTAATTGA+AGG | - | chr8.1:29535760-29535779 | MS.gene76344:CDS | 25.0% |
| ! | CCTTATAAAAAATCACAACA+TGG | - | chr8.1:29537050-29537069 | MS.gene76344:CDS | 25.0% |
| ! | TATATAGATTCTCATGGTTT+GGG | - | chr8.1:29536657-29536676 | MS.gene76344:CDS | 25.0% |
| ! | TTTGACTATATAGATTCTCA+TGG | - | chr8.1:29536651-29536670 | MS.gene76344:CDS | 25.0% |
| !!! | ATAAATTTTGATACTCCTCT+TGG | + | chr8.1:29536989-29537008 | None:intergenic | 25.0% |
| !!! | CCATGTTGTGATTTTTTATA+AGG | + | chr8.1:29537053-29537072 | None:intergenic | 25.0% |
| !!! | TAAATTTTGATACTCCTCTT+GGG | + | chr8.1:29536988-29537007 | None:intergenic | 25.0% |
| !!! | TTACTTTTCTTTTTCTGACA+CGG | + | chr8.1:29536210-29536229 | None:intergenic | 25.0% |
| AACAAATGATCCACCATATT+GGG | - | chr8.1:29537321-29537340 | MS.gene76344:CDS | 30.0% | |
| AAGAAAAACAAGAGAACTGA+AGG | - | chr8.1:29535313-29535332 | MS.gene76344:CDS | 30.0% | |
| AAGAAGAAACTAATTGAGGA+AGG | - | chr8.1:29536147-29536166 | MS.gene76344:CDS | 30.0% | |
| AATGAATGTGAAGATGATGA+TGG | - | chr8.1:29535535-29535554 | MS.gene76344:CDS | 30.0% | |
| ATCATCATCTTCACATTCAT+TGG | + | chr8.1:29536109-29536128 | None:intergenic | 30.0% | |
| CATACTTTGTTAGAAAAGGA+GGG | - | chr8.1:29536798-29536817 | MS.gene76344:CDS | 30.0% | |
| CTATATAGATTCTCATGGTT+TGG | - | chr8.1:29536656-29536675 | MS.gene76344:CDS | 30.0% | |
| GAAAGAAGATGAAAAAGAAG+CGG | - | chr8.1:29535560-29535579 | MS.gene76344:CDS | 30.0% | |
| GTGTCAGAAAAAGAAAAGTA+AGG | - | chr8.1:29536209-29536228 | MS.gene76344:CDS | 30.0% | |
| GTTGCGAGATAATATTTGTT+GGG | - | chr8.1:29537076-29537095 | MS.gene76344:CDS | 30.0% | |
| TAAACGCAAACAAGTAACTT+GGG | - | chr8.1:29536536-29536555 | MS.gene76344:CDS | 30.0% | |
| TCAATAAATTCAAGCTCTTC+AGG | + | chr8.1:29536840-29536859 | None:intergenic | 30.0% | |
| TCATACTTTGTTAGAAAAGG+AGG | - | chr8.1:29536797-29536816 | MS.gene76344:CDS | 30.0% | |
| TCATCATCTTCACATTCATT+GGG | + | chr8.1:29535535-29535554 | None:intergenic | 30.0% | |
| TCTCTATCTTTCAACAAGTT+GGG | + | chr8.1:29537404-29537423 | None:intergenic | 30.0% | |
| TGAGCAATTAACATCAACAT+TGG | - | chr8.1:29537157-29537176 | MS.gene76344:CDS | 30.0% | |
| TGCTCATACTTTGTTAGAAA+AGG | - | chr8.1:29536794-29536813 | MS.gene76344:CDS | 30.0% | |
| TGTTGCGAGATAATATTTGT+TGG | - | chr8.1:29537075-29537094 | MS.gene76344:CDS | 30.0% | |
| TTTGATCTTCTCATCTTCTT+CGG | + | chr8.1:29536622-29536641 | None:intergenic | 30.0% | |
| ! | AAGAAGCACAAACTAATTGA+AGG | - | chr8.1:29535952-29535971 | MS.gene76344:CDS | 30.0% |
| ! | TCTTCTTCATTTTCTTTCCT+TGG | + | chr8.1:29536134-29536153 | None:intergenic | 30.0% |
| ! | TGTAATGACTTTAGAAGCAA+TGG | - | chr8.1:29535991-29536010 | MS.gene76344:CDS | 30.0% |
| !! | TCTTTTTCATCTTCTTTCCT+TGG | + | chr8.1:29535558-29535577 | None:intergenic | 30.0% |
| !! | TCTTTTTCGTCTTTTTTCCT+TGG | + | chr8.1:29535936-29535955 | None:intergenic | 30.0% |
| AAATGTCAAATTACACCCTG+AGG | - | chr8.1:29536704-29536723 | MS.gene76344:CDS | 35.0% | |
| AAGAAACTAATTGAGGAAGG+TGG | - | chr8.1:29536150-29536169 | MS.gene76344:CDS | 35.0% | |
| AATGAAACTCCTAGCAATGA+AGG | - | chr8.1:29536369-29536388 | MS.gene76344:CDS | 35.0% | |
| AATGAATGTGAAGATGACCA+AGG | - | chr8.1:29535916-29535935 | MS.gene76344:CDS | 35.0% | |
| AATTGAAGGAAACACACTGA+AGG | - | chr8.1:29535774-29535793 | MS.gene76344:CDS | 35.0% | |
| AATTGAAGGAAACACACTTG+AGG | - | chr8.1:29535588-29535607 | MS.gene76344:CDS | 35.0% | |
| ACAAATGATCCACCATATTG+GGG | - | chr8.1:29537322-29537341 | MS.gene76344:CDS | 35.0% | |
| ACATTGCTTTCGGACATATA+TGG | + | chr8.1:29535367-29535386 | None:intergenic | 35.0% | |
| AGAAACTAATTGAGGAAGGT+GGG | - | chr8.1:29536151-29536170 | MS.gene76344:CDS | 35.0% | |
| AGAAGCAATGAAGGTGATAA+TGG | - | chr8.1:29535625-29535644 | MS.gene76344:CDS | 35.0% | |
| CAGATCAGTTACATTGCTTT+CGG | + | chr8.1:29535377-29535396 | None:intergenic | 35.0% | |
| CCAAAGCAAAAAAAGGACAT+TGG | - | chr8.1:29536961-29536980 | MS.gene76344:CDS | 35.0% | |
| CTAAACGCAAACAAGTAACT+TGG | - | chr8.1:29536535-29536554 | MS.gene76344:CDS | 35.0% | |
| CTCTATCTTTCAACAAGTTG+GGG | + | chr8.1:29537403-29537422 | None:intergenic | 35.0% | |
| CTCTCTATCTTTCAACAAGT+TGG | + | chr8.1:29537405-29537424 | None:intergenic | 35.0% | |
| GAACAAATGATCCACCATAT+TGG | - | chr8.1:29537320-29537339 | MS.gene76344:CDS | 35.0% | |
| GATAGACGCTTTATATGCTT+TGG | - | chr8.1:29537220-29537239 | MS.gene76344:CDS | 35.0% | |
| GTCACTGAAGTTAATGATCA+AGG | - | chr8.1:29536282-29536301 | MS.gene76344:CDS | 35.0% | |
| GTCACTGATGTTAATGATCA+AGG | - | chr8.1:29536414-29536433 | MS.gene76344:CDS | 35.0% | |
| GTTTCATTATGTGCACAGTT+AGG | + | chr8.1:29536357-29536376 | None:intergenic | 35.0% | |
| TTATGTGTGCTTTCTTCACT+TGG | + | chr8.1:29535874-29535893 | None:intergenic | 35.0% | |
| TTGATCTTCTCATCTTCTTC+GGG | + | chr8.1:29536621-29536640 | None:intergenic | 35.0% | |
| ! | AAGTGAAGAAAGCACACATA+AGG | - | chr8.1:29535873-29535892 | MS.gene76344:CDS | 35.0% |
| ! | AATATTTGTTGGGAGGCAAT+TGG | - | chr8.1:29537086-29537105 | MS.gene76344:CDS | 35.0% |
| ! | AATGACTTTAGAAGCAATGG+AGG | - | chr8.1:29535994-29536013 | MS.gene76344:CDS | 35.0% |
| ! | AGAAGCAATGATGATGAAGA+TGG | - | chr8.1:29535811-29535830 | MS.gene76344:CDS | 35.0% |
| ! | CATATAAAGCGTCTATCAAG+CGG | + | chr8.1:29537217-29537236 | None:intergenic | 35.0% |
| ! | TCACCATTCCTTTTGATCAA+AGG | + | chr8.1:29535400-29535419 | None:intergenic | 35.0% |
| !! | AATCACAGGTTTGTTGTCAA+AGG | + | chr8.1:29537429-29537448 | None:intergenic | 35.0% |
| !! | CAACTTGTTGAAAGATAGAG+AGG | - | chr8.1:29537403-29537422 | MS.gene76344:CDS | 35.0% |
| !!! | CCAATGTCCTTTTTTTGCTT+TGG | + | chr8.1:29536964-29536983 | None:intergenic | 35.0% |
| !!! | GTTCTCTTGTTTTTCTTGCT+CGG | + | chr8.1:29535310-29535329 | None:intergenic | 35.0% |
| AAACCTAGATCTGCTTGAGA+GGG | - | chr8.1:29537019-29537038 | MS.gene76344:CDS | 40.0% | |
| AAATTACACCCTGAGGTTAG+CGG | - | chr8.1:29536711-29536730 | MS.gene76344:CDS | 40.0% | |
| AACCTGCTCAATGAATGACT+TGG | + | chr8.1:29537357-29537376 | None:intergenic | 40.0% | |
| AAGAAGCGGAAGCTAATTGA+AGG | - | chr8.1:29535574-29535593 | MS.gene76344:CDS | 40.0% | |
| AATCTACTTGAGCATAGGTC+TGG | - | chr8.1:29537275-29537294 | MS.gene76344:CDS | 40.0% | |
| ACTCCTAACTCTGCACATAA+TGG | - | chr8.1:29536507-29536526 | MS.gene76344:CDS | 40.0% | |
| CTGATCTGCCTTTGATCAAA+AGG | - | chr8.1:29535389-29535408 | MS.gene76344:CDS | 40.0% | |
| CTGCCTTTGATCAAAAGGAA+TGG | - | chr8.1:29535394-29535413 | MS.gene76344:CDS | 40.0% | |
| CTTTGTTAGAAAAGGAGGGA+AGG | - | chr8.1:29536802-29536821 | MS.gene76344:CDS | 40.0% | |
| GAAAAGACCTATGAGCAACA+TGG | - | chr8.1:29536858-29536877 | MS.gene76344:CDS | 40.0% | |
| GAATGTGAAGATGATGACCA+AGG | - | chr8.1:29535538-29535557 | MS.gene76344:CDS | 40.0% | |
| GAGGAGAAGTTATGTTGTGA+CGG | - | chr8.1:29536570-29536589 | MS.gene76344:CDS | 40.0% | |
| GAGTGACGAAAGCAAACATA+AGG | - | chr8.1:29535684-29535703 | MS.gene76344:CDS | 40.0% | |
| GTTCCATTATGTGCAGAGTT+AGG | + | chr8.1:29536513-29536532 | None:intergenic | 40.0% | |
| TAAACCTAGATCTGCTTGAG+AGG | - | chr8.1:29537018-29537037 | MS.gene76344:CDS | 40.0% | |
| TATTCTTCTTCGTCTTGCCT+TGG | + | chr8.1:29536185-29536204 | None:intergenic | 40.0% | |
| ! | ACATCAACATTGGTTGCTAG+TGG | - | chr8.1:29537167-29537186 | MS.gene76344:CDS | 40.0% |
| ! | GCGAGATAATATTTGTTGGG+AGG | - | chr8.1:29537079-29537098 | MS.gene76344:CDS | 40.0% |
| ! | TTGCAAAAAGCATACCTGAG+AGG | - | chr8.1:29536748-29536767 | MS.gene76344:CDS | 40.0% |
| AAAAGGACATTGGTCCCAAG+AGG | - | chr8.1:29536971-29536990 | MS.gene76344:CDS | 45.0% | |
| AAAAGTGAAGATGGCGAGCA+AGG | - | chr8.1:29535442-29535461 | MS.gene76344:CDS | 45.0% | |
| AACACACTTGAGGAAGGTGA+CGG | - | chr8.1:29535598-29535617 | MS.gene76344:CDS | 45.0% | |
| AAGAGAACTGAAGGCGAAGA+GGG | - | chr8.1:29535322-29535341 | MS.gene76344:CDS | 45.0% | |
| ACTAGCTCCATGTTGCTCAT+AGG | + | chr8.1:29536868-29536887 | None:intergenic | 45.0% | |
| AGAAGCAATGGAGGTGAAGA+GGG | - | chr8.1:29536003-29536022 | MS.gene76344:CDS | 45.0% | |
| AGTAACTTGGGCTGATGAAG+TGG | - | chr8.1:29536548-29536567 | MS.gene76344:CDS | 45.0% | |
| ATGAGCAACATGGAGCTAGT+TGG | - | chr8.1:29536868-29536887 | MS.gene76344:CDS | 45.0% | |
| CATATATGGATCGCCTCCAT+TGG | + | chr8.1:29535353-29535372 | None:intergenic | 45.0% | |
| CGTCGATACACACTCTCATA+AGG | + | chr8.1:29536774-29536793 | None:intergenic | 45.0% | |
| GAAGGAAACACACTTGAGGA+AGG | - | chr8.1:29535592-29535611 | MS.gene76344:CDS | 45.0% | |
| GAATGTGAAGCTGATGACCA+AGG | - | chr8.1:29535730-29535749 | MS.gene76344:CDS | 45.0% | |
| GGGACAATCTACTTGAGCAT+AGG | - | chr8.1:29537270-29537289 | MS.gene76344:CDS | 45.0% | |
| TAGAAGCAATGGAGGTGAAG+AGG | - | chr8.1:29536002-29536021 | MS.gene76344:CDS | 45.0% | |
| TCAGCATCACCTTCATTGCT+AGG | + | chr8.1:29536381-29536400 | None:intergenic | 45.0% | |
| TGATGTGTGCAGGAAACGAT+GGG | - | chr8.1:29537298-29537317 | MS.gene76344:CDS | 45.0% | |
| TTTGGGTAAAAGTCGAGCTC+AGG | - | chr8.1:29536908-29536927 | MS.gene76344:CDS | 45.0% | |
| ! | AAAAGGAGGGAAGGTGTAAG+TGG | - | chr8.1:29536811-29536830 | MS.gene76344:CDS | 45.0% |
| ! | CATTGGTTGCTAGTGGTGAT+TGG | - | chr8.1:29537174-29537193 | MS.gene76344:CDS | 45.0% |
| ! | CTGCTCAATGAATGACTTGG+CGG | + | chr8.1:29537354-29537373 | None:intergenic | 45.0% |
| ! | GTGCATTTTGCTGCAAGAAG+TGG | - | chr8.1:29537132-29537151 | MS.gene76344:CDS | 45.0% |
| !! | GACGGCTTTAGAAGCAATGA+AGG | - | chr8.1:29535616-29535635 | MS.gene76344:CDS | 45.0% |
| !! | TGTGAAGATGATGATGGCCA+AGG | - | chr8.1:29536114-29536133 | MS.gene76344:CDS | 45.0% |
| AACTTGGGCTGATGAAGTGG+AGG | - | chr8.1:29536551-29536570 | MS.gene76344:CDS | 50.0% | |
| ACACTCTCATAAGGCCTCTC+AGG | + | chr8.1:29536765-29536784 | None:intergenic | 50.0% | |
| ACGGCTTATTGCGTGGGAAA+AGG | - | chr8.1:29536589-29536608 | MS.gene76344:CDS | 50.0% | |
| AGAGCAGTAGCTGACGCTTT+GGG | - | chr8.1:29536891-29536910 | MS.gene76344:CDS | 50.0% | |
| CAAGAGAACTGAAGGCGAAG+AGG | - | chr8.1:29535321-29535340 | MS.gene76344:CDS | 50.0% | |
| CGCCAAGTCATTCATTGAGC+AGG | - | chr8.1:29537352-29537371 | MS.gene76344:CDS | 50.0% | |
| GAAGAGGGTGAAACCAATGG+AGG | - | chr8.1:29535337-29535356 | MS.gene76344:CDS | 50.0% | |
| GAGCTCAGGTAAAAGATGCG+TGG | - | chr8.1:29536922-29536941 | MS.gene76344:CDS | 50.0% | |
| GGAGGCAATTGGTCACAAGT+TGG | - | chr8.1:29537097-29537116 | MS.gene76344:CDS | 50.0% | |
| GGGAGACGAAGGTCTTGATA+TGG | - | chr8.1:29536677-29536696 | MS.gene76344:CDS | 50.0% | |
| GTGATGTGTGCAGGAAACGA+TGG | - | chr8.1:29537297-29537316 | MS.gene76344:CDS | 50.0% | |
| GTTGTGACGGCTTATTGCGT+GGG | - | chr8.1:29536583-29536602 | MS.gene76344:CDS | 50.0% | |
| TAGCCCTCTCAAGCAGATCT+AGG | + | chr8.1:29537025-29537044 | None:intergenic | 50.0% | |
| TGTTGTGACGGCTTATTGCG+TGG | - | chr8.1:29536582-29536601 | MS.gene76344:CDS | 50.0% | |
| ! | ATAGGTCTGGTGATGTGTGC+AGG | - | chr8.1:29537288-29537307 | MS.gene76344:CDS | 50.0% |
| ! | TCTCATGGTTTGGGAGACGA+AGG | - | chr8.1:29536666-29536685 | MS.gene76344:CDS | 50.0% |
| ! | TGCTTTGGATGCTTGCTGCA+TGG | - | chr8.1:29537235-29537254 | MS.gene76344:CDS | 50.0% |
| ! | TTTGGATGCTTGCTGCATGG+AGG | - | chr8.1:29537238-29537257 | MS.gene76344:CDS | 50.0% |
| !! | GAAGGTGATAATGGCGAGCA+GGG | - | chr8.1:29535634-29535653 | MS.gene76344:CDS | 50.0% |
| !! | TGAAGGTGATAATGGCGAGC+AGG | - | chr8.1:29535633-29535652 | MS.gene76344:CDS | 50.0% |
| CTGCATGGAGGAGGTAGACT+GGG | - | chr8.1:29537250-29537269 | MS.gene76344:CDS | 55.0% | |
| GAAGGTGGGAATCGTGACCA+AGG | - | chr8.1:29536165-29536184 | MS.gene76344:CDS | 55.0% | |
| GAGAGCAGTAGCTGACGCTT+TGG | - | chr8.1:29536890-29536909 | MS.gene76344:CDS | 55.0% | |
| GGCGAAGAGGGTGAAACCAA+TGG | - | chr8.1:29535334-29535353 | MS.gene76344:CDS | 55.0% | |
| TCCAACAGCCGCTAACCTCA+GGG | + | chr8.1:29536722-29536741 | None:intergenic | 55.0% | |
| TTCCAACAGCCGCTAACCTC+AGG | + | chr8.1:29536723-29536742 | None:intergenic | 55.0% | |
| ACCCTGAGGTTAGCGGCTGT+TGG | - | chr8.1:29536718-29536737 | MS.gene76344:CDS | 60.0% | |
| CGGCATGCTCCCCAATATGG+TGG | + | chr8.1:29537334-29537353 | None:intergenic | 60.0% | |
| GCTGCATGGAGGAGGTAGAC+TGG | - | chr8.1:29537249-29537268 | MS.gene76344:CDS | 60.0% | |
| TGGCGGCATGCTCCCCAATA+TGG | + | chr8.1:29537337-29537356 | None:intergenic | 60.0% | |
| ! | GGATGCTTGCTGCATGGAGG+AGG | - | chr8.1:29537241-29537260 | MS.gene76344:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 29535298 | 29537454 | 29535298 | ID=MS.gene76344 |
| chr8.1 | mRNA | 29535298 | 29537454 | 29535298 | ID=MS.gene76344.t1;Parent=MS.gene76344 |
| chr8.1 | exon | 29535298 | 29537454 | 29535298 | ID=MS.gene76344.t1.exon1;Parent=MS.gene76344.t1 |
| chr8.1 | CDS | 29535298 | 29537454 | 29535298 | ID=cds.MS.gene76344.t1;Parent=MS.gene76344.t1 |
| Gene Sequence |
| Protein sequence |
