AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78129


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78129.t1 MTR_4g055420 96.410 195 7 0 4 198 2 196 3.33e-141 392
MS.gene78129.t1 MTR_2g084805 54.857 175 67 5 17 184 8 177 2.90e-57 179
MS.gene78129.t1 MTR_4g102040 43.077 130 58 2 71 198 57 172 6.37e-28 103
MS.gene78129.t1 MTR_5g011750 51.724 87 42 0 71 157 26 112 1.31e-24 93.6
MS.gene78129.t1 MTR_5g011770 40.741 81 48 0 74 154 117 197 1.01e-14 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78129.t1 AT2G27830 49.254 201 86 7 1 196 1 190 1.34e-51 164
MS.gene78129.t1 AT4G22758 38.462 91 54 1 70 160 119 207 8.23e-13 65.5
MS.gene78129.t1 AT1G70780 30.328 122 79 3 73 190 16 135 1.01e-11 60.5

Find 48 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACTTTCTTCAAGCTTCTT+TGG 0.205520 4.4:+47648393 None:intergenic
AATGAGAAGCTTAAGGAAAT+TGG 0.265022 4.4:-47648438 MS.gene78129:CDS
GCCGACGAAGTTACTCATTA+AGG 0.295975 4.4:-47648903 MS.gene78129:CDS
GAGTAACTTCGTCGGCTGTC+TGG 0.327050 4.4:+47648910 None:intergenic
GCTTCCTTCGACGGCAAGAT+TGG 0.327598 4.4:+47648770 None:intergenic
TTGCCGCACGCTTCCTGTCC+TGG 0.374159 4.4:+47648960 None:intergenic
TAAACGACGCCGTTACGTTA+TGG 0.389603 4.4:+47648358 None:intergenic
AGATAGAAATGAGAAGCTTA+AGG 0.392238 4.4:-47648445 MS.gene78129:CDS
ACAAGGTCCGGCATAGTCTT+TGG 0.414724 4.4:+47648983 None:intergenic
ACTCATTAAGGTTACGATGA+TGG 0.455220 4.4:-47648891 MS.gene78129:CDS
CTCATTAAGGTTACGATGAT+GGG 0.455912 4.4:-47648890 MS.gene78129:CDS
AGTAACTTCGTCGGCTGTCT+GGG 0.474432 4.4:+47648911 None:intergenic
CACGCTTCCTGTCCTGGACA+AGG 0.482528 4.4:+47648966 None:intergenic
TTCCTGTCCTGGACAAGGTC+CGG 0.485224 4.4:+47648971 None:intergenic
GAGTTTCCACATGGCTGTTA+CGG 0.501819 4.4:+47649089 None:intergenic
ATTTGATAGCGGAGGCTGTA+CGG 0.516148 4.4:-47648817 MS.gene78129:CDS
GTTACGATGATGGGCAGCCT+TGG 0.521614 4.4:-47648881 MS.gene78129:CDS
TGCCGGACCTTGTCCAGGAC+AGG 0.523940 4.4:-47648973 MS.gene78129:CDS
GACTATGCCGGACCTTGTCC+AGG 0.528186 4.4:-47648978 MS.gene78129:CDS
CTCGCCTGTGAGTTTCCACA+TGG 0.535722 4.4:+47649080 None:intergenic
CGTGCAAGTGTTAATGTCGA+TGG 0.538374 4.4:-47648855 MS.gene78129:CDS
CGCTATCAAATCTCCAACCG+TGG 0.541253 4.4:+47648829 None:intergenic
CCGGCATAGTCTTTGGCCGC+CGG 0.545096 4.4:+47648990 None:intergenic
AAGCAAGAAGCTTCCTTCGA+CGG 0.546345 4.4:+47648761 None:intergenic
TTTCCACATGGCTGTTACGG+TGG 0.552551 4.4:+47649092 None:intergenic
CCGTCCAATCTTGCCGTCGA+AGG 0.553306 4.4:-47648774 MS.gene78129:CDS
CATCGACATTAACACTTGCA+CGG 0.560991 4.4:+47648856 None:intergenic
ATCGACATTAACACTTGCAC+GGG 0.561462 4.4:+47648857 None:intergenic
GTACGGTTGTATGTTAAAGA+AGG 0.567445 4.4:-47648800 MS.gene78129:CDS
AGCTTAAGGAAATTGGATCA+AGG 0.569028 4.4:-47648431 MS.gene78129:CDS
CCTTCGACGGCAAGATTGGA+CGG 0.570300 4.4:+47648774 None:intergenic
GGCAATGACTACGTTGCCAC+CGG 0.575365 4.4:-47648942 MS.gene78129:CDS
TTAACACTTGCACGGGACCA+AGG 0.594751 4.4:+47648864 None:intergenic
ACAGCCATGTGGAAACTCAC+AGG 0.595709 4.4:-47649084 MS.gene78129:CDS
CCGGCGGCCAAAGACTATGC+CGG 0.600487 4.4:-47648990 MS.gene78129:CDS
ATGTCGATGGAGTCCACGGT+TGG 0.606238 4.4:-47648842 MS.gene78129:CDS
GTTAATGTCGATGGAGTCCA+CGG 0.622963 4.4:-47648846 MS.gene78129:CDS
AAGCTTGAAGAAAGTGACAA+TGG 0.627431 4.4:-47648387 MS.gene78129:CDS
ACCTTAATGAGTAACTTCGT+CGG 0.631958 4.4:+47648902 None:intergenic
GGTTGGAGATTTGATAGCGG+AGG 0.634372 4.4:-47648825 MS.gene78129:CDS
CACTGTCTAGTGCAAAACTC+CGG 0.637523 4.4:-47649009 MS.gene78129:CDS
TGTCCAGGACAGGAAGCGTG+CGG 0.659036 4.4:-47648963 MS.gene78129:CDS
CTTGAAGAAAGTGACAATGG+TGG 0.660090 4.4:-47648384 MS.gene78129:CDS
CACGGTTGGAGATTTGATAG+CGG 0.675641 4.4:-47648828 MS.gene78129:CDS
TGGTGGAGACCATAACGTAA+CGG 0.684404 4.4:-47648367 MS.gene78129:CDS
AAACCACCGTAACAGCCATG+TGG 0.698169 4.4:-47649095 MS.gene78129:CDS
CATGTGGAAACTCACAGGCG+AGG 0.700520 4.4:-47649079 MS.gene78129:CDS
TGTCTAGTGCAAAACTCCGG+CGG 0.740470 4.4:-47649006 MS.gene78129:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAGTTATTATTTTTTGT+TGG - chr4.4:47648898-47648917 MS.gene78129:CDS 15.0%
!!! TATTAGGTCTTTATTTTTTT+TGG - chr4.4:47648828-47648847 MS.gene78129:CDS 15.0%
!! TGAACTATGAAAACTAAAAT+TGG + chr4.4:47648709-47648728 None:intergenic 20.0%
!!! AGTTATTATTTTTTGTTGGA+GGG - chr4.4:47648902-47648921 MS.gene78129:CDS 20.0%
!!! TAGTTATTATTTTTTGTTGG+AGG - chr4.4:47648901-47648920 MS.gene78129:CDS 20.0%
! ACTAGAACAACGTAAATATT+AGG - chr4.4:47648812-47648831 MS.gene78129:CDS 25.0%
AATGAGAAGCTTAAGGAAAT+TGG - chr4.4:47648961-47648980 MS.gene78129:CDS 30.0%
AGATAGAAATGAGAAGCTTA+AGG - chr4.4:47648954-47648973 MS.gene78129:CDS 30.0%
TATGACGTATCTAATTTCTC+TGG - chr4.4:47648773-47648792 MS.gene78129:CDS 30.0%
! GAGAAATCATCATCTTTTCA+TGG - chr4.4:47648353-47648372 MS.gene78129:CDS 30.0%
!!! TTGATTTGTTTTATGAGTGC+AGG - chr4.4:47648928-47648947 MS.gene78129:CDS 30.0%
AAGCTTGAAGAAAGTGACAA+TGG - chr4.4:47649012-47649031 MS.gene78129:CDS 35.0%
ACCTTAATGAGTAACTTCGT+CGG + chr4.4:47648500-47648519 None:intergenic 35.0%
ACTCATTAAGGTTACGATGA+TGG - chr4.4:47648508-47648527 MS.gene78129:intron 35.0%
AGCTTAAGGAAATTGGATCA+AGG - chr4.4:47648968-47648987 MS.gene78129:CDS 35.0%
CTCATTAAGGTTACGATGAT+GGG - chr4.4:47648509-47648528 MS.gene78129:intron 35.0%
GTACGGTTGTATGTTAAAGA+AGG - chr4.4:47648599-47648618 MS.gene78129:intron 35.0%
TCACTTTCTTCAAGCTTCTT+TGG + chr4.4:47649009-47649028 None:intergenic 35.0%
! CAAGTTGAAAAAGTGAGCAA+AGG - chr4.4:47649078-47649097 MS.gene78129:CDS 35.0%
! GAAAAAGTGAGCAAAGGTTT+TGG - chr4.4:47649084-47649103 MS.gene78129:CDS 35.0%
!! GCAAAGGTTTTGGTCTTTTA+AGG - chr4.4:47649094-47649113 MS.gene78129:CDS 35.0%
ATCGACATTAACACTTGCAC+GGG + chr4.4:47648545-47648564 None:intergenic 40.0%
CATCGACATTAACACTTGCA+CGG + chr4.4:47648546-47648565 None:intergenic 40.0%
CTTGAAGAAAGTGACAATGG+TGG - chr4.4:47649015-47649034 MS.gene78129:CDS 40.0%
GTACCATTGTAGATGCTCTT+AGG - chr4.4:47648853-47648872 MS.gene78129:CDS 40.0%
!! AGACCTAAGAGCATCTACAA+TGG + chr4.4:47648859-47648878 None:intergenic 40.0%
!!! GCTCACTTTTTCAACTTGTC+TGG + chr4.4:47649076-47649095 None:intergenic 40.0%
AAGCAAGAAGCTTCCTTCGA+CGG + chr4.4:47648641-47648660 None:intergenic 45.0%
CACGGTTGGAGATTTGATAG+CGG - chr4.4:47648571-47648590 MS.gene78129:intron 45.0%
CACTGTCTAGTGCAAAACTC+CGG - chr4.4:47648390-47648409 MS.gene78129:CDS 45.0%
CGTGCAAGTGTTAATGTCGA+TGG - chr4.4:47648544-47648563 MS.gene78129:intron 45.0%
GAGTTTCCACATGGCTGTTA+CGG + chr4.4:47648313-47648332 None:intergenic 45.0%
GCCGACGAAGTTACTCATTA+AGG - chr4.4:47648496-47648515 MS.gene78129:intron 45.0%
GTTAATGTCGATGGAGTCCA+CGG - chr4.4:47648553-47648572 MS.gene78129:intron 45.0%
TAAACGACGCCGTTACGTTA+TGG + chr4.4:47649044-47649063 None:intergenic 45.0%
TGGTGGAGACCATAACGTAA+CGG - chr4.4:47649032-47649051 MS.gene78129:CDS 45.0%
!! ATTTGATAGCGGAGGCTGTA+CGG - chr4.4:47648582-47648601 MS.gene78129:intron 45.0%
AAACCACCGTAACAGCCATG+TGG - chr4.4:47648304-47648323 MS.gene78129:CDS 50.0%
ACAAGGTCCGGCATAGTCTT+TGG + chr4.4:47648419-47648438 None:intergenic 50.0%
ACAGCCATGTGGAAACTCAC+AGG - chr4.4:47648315-47648334 MS.gene78129:CDS 50.0%
CGCTATCAAATCTCCAACCG+TGG + chr4.4:47648573-47648592 None:intergenic 50.0%
GGCTGTCTGGGAAAAAACTC+CGG + chr4.4:47648479-47648498 None:intergenic 50.0%
TGTCTAGTGCAAAACTCCGG+CGG - chr4.4:47648393-47648412 MS.gene78129:CDS 50.0%
TGTCTGGGAAAAAACTCCGG+TGG + chr4.4:47648476-47648495 None:intergenic 50.0%
TTAACACTTGCACGGGACCA+AGG + chr4.4:47648538-47648557 None:intergenic 50.0%
TTTCCACATGGCTGTTACGG+TGG + chr4.4:47648310-47648329 None:intergenic 50.0%
! AGTAACTTCGTCGGCTGTCT+GGG + chr4.4:47648491-47648510 None:intergenic 50.0%
!! GGTTGGAGATTTGATAGCGG+AGG - chr4.4:47648574-47648593 MS.gene78129:intron 50.0%
ATGTCGATGGAGTCCACGGT+TGG - chr4.4:47648557-47648576 MS.gene78129:intron 55.0%
CATGTGGAAACTCACAGGCG+AGG - chr4.4:47648320-47648339 MS.gene78129:CDS 55.0%
CCTTCGACGGCAAGATTGGA+CGG + chr4.4:47648628-47648647 None:intergenic 55.0%
CTCGCCTGTGAGTTTCCACA+TGG + chr4.4:47648322-47648341 None:intergenic 55.0%
GCTTCCTTCGACGGCAAGAT+TGG + chr4.4:47648632-47648651 None:intergenic 55.0%
GGCAATGACTACGTTGCCAC+CGG - chr4.4:47648457-47648476 MS.gene78129:intron 55.0%
TTCCTGTCCTGGACAAGGTC+CGG + chr4.4:47648431-47648450 None:intergenic 55.0%
! GAGTAACTTCGTCGGCTGTC+TGG + chr4.4:47648492-47648511 None:intergenic 55.0%
!! GTTACGATGATGGGCAGCCT+TGG - chr4.4:47648518-47648537 MS.gene78129:intron 55.0%
CACGCTTCCTGTCCTGGACA+AGG + chr4.4:47648436-47648455 None:intergenic 60.0%
CCGTCCAATCTTGCCGTCGA+AGG - chr4.4:47648625-47648644 MS.gene78129:intron 60.0%
GACTATGCCGGACCTTGTCC+AGG - chr4.4:47648421-47648440 MS.gene78129:CDS 60.0%
! TGTCCAGGACAGGAAGCGTG+CGG - chr4.4:47648436-47648455 MS.gene78129:CDS 60.0%
CCGGCATAGTCTTTGGCCGC+CGG + chr4.4:47648412-47648431 None:intergenic 65.0%
CCGGCGGCCAAAGACTATGC+CGG - chr4.4:47648409-47648428 MS.gene78129:CDS 65.0%
TGCCGGACCTTGTCCAGGAC+AGG - chr4.4:47648426-47648445 MS.gene78129:CDS 65.0%
TTGCCGCACGCTTCCTGTCC+TGG + chr4.4:47648442-47648461 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 47648284 47649137 47648284 ID=MS.gene78129
chr4.4 mRNA 47648284 47649137 47648284 ID=MS.gene78129.t1;Parent=MS.gene78129
chr4.4 exon 47648729 47649137 47648729 ID=MS.gene78129.t1.exon1;Parent=MS.gene78129.t1
chr4.4 CDS 47648729 47649137 47648729 ID=cds.MS.gene78129.t1;Parent=MS.gene78129.t1
chr4.4 exon 47648284 47648471 47648284 ID=MS.gene78129.t1.exon2;Parent=MS.gene78129.t1
chr4.4 CDS 47648284 47648471 47648284 ID=cds.MS.gene78129.t1;Parent=MS.gene78129.t1
Gene Sequence

>MS.gene78129

ATGTCAAACTCAAACGTAAAAAACCACCGTAACAGCCATGTGGAAACTCACAGGCGAGGAAGATTAACAGAGAAATCATCATCTTTTCATGGTCATACTTCTGCAACACTGTCTAGTGCAAAACTCCGGCGGCCAAAGACTATGCCGGACCTTGTCCAGGACAGGAAGCGTGCGGCAATGACTACGTTGCCACCGGAGTTTTTTCCCAGACAGCCGACGAAGTTACTCATTAAGGTTACGATGATGGGCAGCCTTGGTCCCGTGCAAGTGTTAATGTCGATGGAGTCCACGGTTGGAGATTTGATAGCGGAGGCTGTACGGTTGTATGTTAAAGAAGGTCGCCGTCCAATCTTGCCGTCGAAGGAAGCTTCTTGCTTTGATCTTCATTACTCTCAGTTCAGCTTAGAAAGTAAGTTATATTTCCAATTTTAGTTTTCATAGTTCATTTATTAACAACTGAATTTTCACTTATAGTTCATATAATAGCACTATGACGTATCTAATTTCTCTGGCAACTAAGTTATGCTAACTAGAACAACGTAAATATTAGGTCTTTATTTTTTTTGGTAGTACCATTGTAGATGCTCTTAGGTCTTTATTTAACATATCTTCTAATGTAGTTATTATTTTTTGTTGGAGGGAAATTGATTTGTTTTATGAGTGCAGGTTTAGATAGAAATGAGAAGCTTAAGGAAATTGGATCAAGGAACTTTTTTATGTGTCCAAAGAAGCTTGAAGAAAGTGACAATGGTGGAGACCATAACGTAACGGCGTCGTTTAGTTCATGTTCCAGACAAGTTGAAAAAGTGAGCAAAGGTTTTGGTCTTTTAAGGTGTATAGATTTCTTGATATGA

Protein sequence

>MS.gene78129.t1

MSNSNVKNHRNSHVETHRRGRLTEKSSSFHGHTSATLSSAKLRRPKTMPDLVQDRKRAAMTTLPPEFFPRQPTKLLIKVTMMGSLGPVQVLMSMESTVGDLIAEAVRLYVKEGRRPILPSKEASCFDLHYSQFSLESLDRNEKLKEIGSRNFFMCPKKLEESDNGGDHNVTASFSSCSRQVEKVSKGFGLLRCIDFLI