AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79202


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 0 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTTGTATTTACAGATTA+AGG - 24130:20818-20837 MS.gene79202:intron 20.0%
! ATAATCAACAACAATGATGT+TGG - 24130:21002-21021 MS.gene79202:CDS 25.0%
! GATCTTAGTTATGAAATTGT+AGG - 24130:20697-20716 MS.gene79202:intron 25.0%
! TATTTAGTGTATGTTTGGAT+TGG - 24130:20607-20626 MS.gene79202:intron 25.0%
! TGAATTTAGCAAAATCACTT+TGG - 24130:20633-20652 MS.gene79202:intron 25.0%
! TTATAGTTAAGTTACTACTG+TGG + 24130:20316-20335 None:intergenic 25.0%
!!! AAAAAGCACAATCAATGTTT+TGG - 24130:20869-20888 MS.gene79202:intron 25.0%
!!! TGTGTTATTTAGTGTATGTT+TGG - 24130:20602-20621 MS.gene79202:intron 25.0%
AACAACAATGATGTTGGTAT+TGG - 24130:21008-21027 MS.gene79202:CDS 30.0%
AAGGAATTGAAATCAAAGCT+TGG - 24130:20837-20856 MS.gene79202:intron 30.0%
ATTAAGAGTGGATCAAGTTA+AGG - 24130:20040-20059 MS.gene79202:CDS 30.0%
GAAATCTTATCATGTGAAGA+TGG - 24130:21178-21197 MS.gene79202:intron 30.0%
GTCATAATCCAATTGATCTT+TGG + 24130:20979-20998 None:intergenic 30.0%
TCTTCACATGATAAGATTTC+TGG + 24130:21178-21197 None:intergenic 30.0%
TTCAGAATCCAAAGATCAAT+TGG - 24130:20968-20987 MS.gene79202:CDS 30.0%
TTTCAATCAATGATGTTGGA+TGG - 24130:19961-19980 MS.gene79202:CDS 30.0%
! GAATTGATGTTTCTGAAGAT+GGG + 24130:20944-20963 None:intergenic 30.0%
!! GGCAAGTATTTAACTTTTCT+AGG - 24130:20718-20737 MS.gene79202:intron 30.0%
AAGATTCAGGAGACAACAAA+AGG + 24130:21133-21152 None:intergenic 35.0%
AAGCAGTTGGAAAGAGATTA+TGG - 24130:20194-20213 MS.gene79202:CDS 35.0%
AGAAGAACGTAGATTAAGAG+TGG - 24130:20028-20047 MS.gene79202:CDS 35.0%
AGATCAAGTACTTTCACAAG+AGG + 24130:20682-20701 None:intergenic 35.0%
ATCTTATCATGTGAAGATGG+AGG - 24130:21181-21200 MS.gene79202:intron 35.0%
GAACAAACATGTGTATGGTA+TGG - 24130:19936-19955 MS.gene79202:CDS 35.0%
GGAGTTTCAATCAATGATGT+TGG - 24130:19957-19976 MS.gene79202:CDS 35.0%
TGATCTTTGGATTCTGAACT+AGG + 24130:20966-20985 None:intergenic 35.0%
TTCATCACAAGATTCAAGCA+TGG + 24130:20910-20929 None:intergenic 35.0%
TTTCCAGTAGACTTGAAAGA+CGG - 24130:21047-21066 MS.gene79202:CDS 35.0%
! AGACAACAAAGCTTTTCACA+AGG - 24130:20271-20290 MS.gene79202:CDS 35.0%
! GGAATTGATGTTTCTGAAGA+TGG + 24130:20945-20964 None:intergenic 35.0%
! TGGATGGATTTGAAGAAGAA+GGG - 24130:19977-19996 MS.gene79202:CDS 35.0%
! TTGGATGGATTTGAAGAAGA+AGG - 24130:19976-19995 MS.gene79202:CDS 35.0%
!! CATGATAAGATTTCTGGTAC+TGG + 24130:21172-21191 None:intergenic 35.0%
!!! AATCAATGTTTTGGTGAAGG+AGG - 24130:20878-20897 MS.gene79202:intron 35.0%
!!! CACAATCAATGTTTTGGTGA+AGG - 24130:20875-20894 MS.gene79202:intron 35.0%
AACCAAACAGCTACTTGTCT+AGG + 24130:20146-20165 None:intergenic 40.0%
AAGATGGAAGAACAAGCAGT+TGG - 24130:20181-20200 MS.gene79202:CDS 40.0%
AGAAGCTTGCCATTGAACTT+GGG - 24130:20114-20133 MS.gene79202:CDS 40.0%
AGACTTGAAAGACGGTTCTT+CGG - 24130:21055-21074 MS.gene79202:CDS 40.0%
AGTGGATCAAGTTAAGGCTT+TGG - 24130:20046-20065 MS.gene79202:CDS 40.0%
CAATTCATGGAGGAAGATTC+AGG + 24130:21146-21165 None:intergenic 40.0%
CCAAGGAACAAACATGTGTA+TGG - 24130:19931-19950 MS.gene79202:CDS 40.0%
CCATACACATGTTTGTTCCT+TGG + 24130:19934-19953 None:intergenic 40.0%
GTACTGGAAACAATTCATGG+AGG + 24130:21156-21175 None:intergenic 40.0%
! CAACAAAGCTTTTCACAAGG+AGG - 24130:20274-20293 MS.gene79202:CDS 40.0%
! CTGGTACTGGAAACAATTCA+TGG + 24130:21159-21178 None:intergenic 40.0%
! GCAAGCTTCTCTTTTCTTTC+AGG + 24130:20104-20123 None:intergenic 40.0%
! TAGCTGTTTGGTTCCAAAAC+AGG - 24130:20153-20172 MS.gene79202:CDS 40.0%
!! CAATTTCTTAAGTGCCGATG+AGG - 24130:21208-21227 MS.gene79202:CDS 40.0%
!! GGCTTTGGAGAAAAACTTTG+AGG - 24130:20061-20080 MS.gene79202:CDS 40.0%
AGCCTAGACAAGTAGCTGTT+TGG - 24130:20141-20160 MS.gene79202:CDS 45.0%
GAAAAAGTTGCACGCCTCAT+CGG + 24130:21225-21244 None:intergenic 45.0%
GAGAAGCTTGCCATTGAACT+TGG - 24130:20113-20132 MS.gene79202:CDS 45.0%
GTCAGGACAGTACCATTGTA+GGG + 24130:21267-21286 None:intergenic 45.0%
TCCAAAACAGGAGAGCAAGA+TGG - 24130:20165-20184 MS.gene79202:CDS 45.0%
TCCAGCGCAATTTCTTCATC+CGG - 24130:21086-21105 MS.gene79202:CDS 45.0%
! GAACCGTCTTTCAAGTCTAC+TGG + 24130:21053-21072 None:intergenic 45.0%
! GAAGGGTGTGTTGAAGAAAC+TGG - 24130:19994-20013 MS.gene79202:CDS 45.0%
! TCCATCTTGCTCTCCTGTTT+TGG + 24130:20169-20188 None:intergenic 45.0%
!! TGAGGCGTGCAACTTTTTCT+CGG - 24130:21226-21245 MS.gene79202:CDS 45.0%
AATGGTACTGTCCTGACCAG+TGG - 24130:21270-21289 MS.gene79202:CDS 50.0%
AGCAAGCACCAACCCTACAA+TGG - 24130:21252-21271 MS.gene79202:CDS 50.0%
AGGCTGCAACCCAAGTTCAA+TGG + 24130:20126-20145 None:intergenic 50.0%
CAGCGCAATTTCTTCATCCG+GGG - 24130:21088-21107 MS.gene79202:CDS 50.0%
CCAGCGCAATTTCTTCATCC+GGG - 24130:21087-21106 MS.gene79202:CDS 50.0%
GGACAGTACCATTGTAGGGT+TGG + 24130:21263-21282 None:intergenic 50.0%
GGTCAGGACAGTACCATTGT+AGG + 24130:21268-21287 None:intergenic 50.0%
GTCCTGACCAGTGGAGTTAA+AGG - 24130:21279-21298 MS.gene79202:CDS 50.0%
CCCGGATGAAGAAATTGCGC+TGG + 24130:21090-21109 None:intergenic 55.0%
!! CTGCTGACTCTGAAGAACCC+CGG + 24130:21108-21127 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
24130 gene 19920 21298 19920 ID=MS.gene79202
24130 mRNA 19920 21298 19920 ID=MS.gene79202.t1;Parent=MS.gene79202
24130 exon 21183 21298 21183 ID=MS.gene79202.t1.exon1;Parent=MS.gene79202.t1
24130 CDS 21183 21298 21183 ID=cds.MS.gene79202.t1;Parent=MS.gene79202.t1
24130 exon 20923 21147 20923 ID=MS.gene79202.t1.exon2;Parent=MS.gene79202.t1
24130 CDS 20923 21147 20923 ID=cds.MS.gene79202.t1;Parent=MS.gene79202.t1
24130 exon 19920 20384 19920 ID=MS.gene79202.t1.exon3;Parent=MS.gene79202.t1
24130 CDS 19920 20384 19920 ID=cds.MS.gene79202.t1;Parent=MS.gene79202.t1
Gene Sequence

>MS.gene79202

AGGAACATAGTCCAAGGAACAAACATGTGTATGGTATGGAGTTTCAATCAATGATGTTGGATGGATTTGAAGAAGAAGGGTGTGTTGAAGAAACTGGTCATCATTCTGAGAAGAACGTAGATTAAGAGTGGATCAAGTTAAGGCTTTGGAGAAAAACTTTGAGGTAGAAAACAAGCTTGAACCTGAAAGAAAAGAGAAGCTTGCCATTGAACTTGGGTTGCAGCCTAGACAAGTAGCTGTTTGGTTCCAAAACAGGAGAGCAAGATGGAAGAACAAGCAGTTGGAAAGAGATTATGGTGTTCTTAAAGCTAATTATGATGCTCTTAAGCTCAAGTTTGATGCAATTGCTCAAGACAACAAAGCTTTTCACAAGGAGGTATAAACATCTATAATCCACAGTAGTAACTTAACTATAATTTAGTTTCTGAAGTTATAAGTGCAATAACAACTTAATTAAGTGTTTATAGCATAATCACTTATCATTTAAGTACTTTCGAATAAGCTACTTCATAATATAAGATAGAATAAAGTCACTAGTCAAACTATTTTCATGTAAGTTATTAGTTGTTTTCATAAATTATTCTAAAGCGCTTGTGAAAATAAGCTAAAAACAACTTATTTATAAGTTGTTTCTATAAAAAACAACTTATTTGATATATCAATTCACAGATTTTTAAGCTGTTGTGTTATTTAGTGTATGTTTGGATTGGCAGTGAATTTAGCAAAATCACTTTGGCACTTGAATTTGTAAAGCTATGTCCTCTTGTGAAAGTACTTGATCTTAGTTATGAAATTGTAGGCAAGTATTTAACTTTTCTAGGAACAAATGTATCATAAATTTGAAGTTATTATTGAAAGTGTGTGATTAATTTTCTCTCAAAAACAGAAAGTTGATTGATTTGTTGTATTTACAGATTAAGGAATTGAAATCAAAGCTTGGAGAAGAAGAAAAAAGCACAATCAATGTTTTGGTGAAGGAGGAGTTAACCATGCTTGAATCTTGTGATGAAGACAAGCATAACCCATCTTCAGAAACATCAATTCCTAGTTCAGAATCCAAAGATCAATTGGATTATGACTGCATAATCAACAACAATGATGTTGGTATTGGTGAAACTTCTTCACTCTTTCCAGTAGACTTGAAAGACGGTTCTTCGGACAGCGACTCCAGCGCAATTTCTTCATCCGGGGTTCTTCAGAGTCAGCAGCACCTTTTGTTGTCTCCTGAATCTTCCTCCATGAATTGTTTCCAGTACCAGAAATCTTATCATGTGAAGATGGAGGAGCACAATTTCTTAAGTGCCGATGAGGCGTGCAACTTTTTCTCGGACGAGCAAGCACCAACCCTACAATGGTACTGTCCTGACCAGTGGAGTTAA

Protein sequence

>MS.gene79202.t1

RNIVQGTNMCMVWSFNQUCWMDLKKKGVLKKLVIILRRTKTLRUKTSLNLKEKRSLPLNLGCSLDKULFGSKTGEQDGRTSSWKEIMVFLKLIMMLLSSSLMQLLKTTKLFTRRLRNUNQSLEKKKKAQSMFWURRSUPCLNLVMKTSITHLQKHQFLVQNPKINWIMTAUSTTMMLVLVKLLHSFQUTUKTVLRTATPAQFLHPGFFRVSSTFCCLLNLPPUIVSSTRNLIMURWRSTISUVPMRRATFSRTSKHQPYNGTVLTSGV